Integrative In Silico Analysis of Hypothetical Proteins from Stenotrophomonas maltophilia NCTC10958: Insights into Environmental Adaptation and Virulence Factors

Integrative In Silico Analysis of Hypothetical Proteins from Stenotrophomonas maltophilia NCTC10958: Insights into Environmental Adaptation and Virulence Factors | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Integrative In Silico Analysis of Hypothetical Proteins from Stenotrophomonas maltophilia NCTC10958: Insights into Environmental Adaptation and Virulence Factors Iman Hussein Fadhiladeen Alnaqshabandy This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-7217113/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Antimicrobial resistance has become one of the most significant challenges for the public health domain. The escalation of multidrug resistance worldwide, particularly among Gram-negative bacteria, is a global concern. Among these, Stenotrophomonas maltophilia (S. maltophilia) is the most prevalent pathogen that affects immunocompromised patients, specifically those in intensive care units (ICUs). This pathogen exhibits resistance to a broad spectrum of antibiotics through mechanisms such as enzymatic degradation, efflux pumps, and biofilm formation. Although S. maltophilia is associated with few virulence factors, its pathogenicity is enhanced by a small number of potent determinants including hydrolytic enzymes and quorum sensing systems. The S. maltophilia pathogen has become a central focus study due to its increasing incidence in hospital environments, particularly during the COVID-19 pandemic, in addition to its resistance to treatment strategies. Understanding the involvement of virulence factors in bacterial pathogenicity in depth is essential for therapeutic developments. This study aimed to characterize 61 hypothetical proteins (HPs) from S. maltophilia NCTC10958. Functional annotations suggested potential involvement of these proteins in virulence mechanisms, environmental adaptation, and antibiotic resistance. The results predict several crucial virulence determinants and proteins that are essential in bacterial resistance and adaptation. These findings may aid the development of novel therapeutic strategies and the discovery of alternative drugs to combat infections caused by this multidrug-resistant pathogen. Stenotrophomonas maltophilia hypothetical proteins antimicrobial resistance virulence factors Full Text Supplementary Files SupplementaryData.xlsx Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-7217113","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":519351241,"identity":"701bdff8-9c79-4c2f-b378-dceebcd27db7","order_by":0,"name":"Iman Hussein Fadhiladeen 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The escalation of multidrug resistance worldwide, particularly among Gram-negative bacteria, is a global concern. Among these, Stenotrophomonas maltophilia (S. maltophilia) is the most prevalent pathogen that affects immunocompromised patients, specifically those in intensive care units (ICUs). This pathogen exhibits resistance to a broad spectrum of antibiotics through mechanisms such as enzymatic degradation, efflux pumps, and biofilm formation. Although S. maltophilia is associated with few virulence factors, its pathogenicity is enhanced by a small number of potent determinants including hydrolytic enzymes and quorum sensing systems. The S. maltophilia pathogen has become a central focus study due to its increasing incidence in hospital environments, particularly during the COVID-19 pandemic, in addition to its resistance to treatment strategies. Understanding the involvement of virulence factors in bacterial pathogenicity in depth is essential for therapeutic developments. 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