Sero epidemiology of Cattle Brucellosis and Associated Risk Factors in Amibara District of Afar Region, Ethiopia

Sero epidemiology of Cattle Brucellosis and Associated Risk Factors in Amibara District of Afar Region, Ethiopia | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Sero epidemiology of Cattle Brucellosis and Associated Risk Factors in Amibara District of Afar Region, Ethiopia Fekadu Gutema Wegi, Juhar Tesfaye Bekele This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-984061/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Bovine brucellosis is the most common but under reported bacterial diseases known to create a serious socio-economic problem in both intensive and extensive livestock production systems. A cross-sectional study was conducted to determine seroprevalence and associated risk factors of cattle brucellosis in Amibara district of Afar region, Ethiopia from October 2019 to May 2020. A total of 181 cattle sera were collected and screened using Rose Bengal Plate Test (RBPT) and reactive samples were further confirmed by complement fixation test (CFT). Risk factors associated with cattle brucellosis were assessed during serum sample collection using data collection format. As a result, the overall seroprevalence of cattle brucellosis was 10.5% and 2.2% by RBPT and CFT respectively. Assessment of potential risk factors showed that, Age (χ2=6.77, p=0.021), number of parity (χ2=9.433, p=0.004), abortion history (χ2=16, p=0.002) and history of placental retention (χ2=19.1, p=0.003) showed statistically significant association with brucellosis seropositivity in cattle. Based on firth’s bias reduced logistic regression analysis, only multiparous animal (OR=10.68, P=0.0042, 95% CI=-1.19-7.595) and animals with placental retention (OR=72.72, P=0.0026, 95% CI=1.46-9.272) showed statistically significantly association with brucella infection in cattle. In conclusion, the results of the current study indicate the presence of brucellosis in cattle in Amibara district of Afar region, Ethiopia. Hence, implementing preventive measures such as developing vaccination strategy, regular screening and culling of the reactive animal is important to create diseases free herd. Large Animal Medicine Amibara Afar Brucellosis Cattle Ethiopia Seroprevalence Figures Figure 1 Introduction Ethiopia stands first in Africa based on cattle populations despite gaining minimum return from this resource because of managements, policy and different infectious diseases ( Edao et al., 2018 ). Bovine brucellosis is the most common but under reported bacterial diseases which is known to creates a serious socio-economic problem in both intensive and extensive livestock production systems(Deka et al., 2019 ). It is caused by Brucella spp and manifests itself as abortion and infertility in domestic and wild animal species and reduced milk production (Mathew et al., 2015 ). In cattle the disease is mainly caused by B. abortus and characterized by inflammation of the genitals and foetal membranes, abortions, sterility and lesions in the lymphatic system and joints(Corbel, 2006 ). Developing countries with limited resources have not yet fully launched programs featuring any aspects of brucellosis intervention, since they are facing other priority diseases that are more spectacular (Lakew et al .,2019). Risk factors associated with brucellosis can be categorized into management, animal, and environmental factors(AR et al., 2014 ). Sources of infection for the transmission of the bovine brucellosis are aborted fetuses, retained fetal membranes, and vaginal discharges and milk from infected animals(Edao et al., 2018 ). In human, brucellosis is one of the most common bacterial zoonotic infections, but remains under reported disease in Ethiopia due to the absence of diagnostic facility in public hospitals. B . melitensis , B . abortus , and B . suis are known to induce significant public health problems (Massis et al., 2019 ). The risks of zoonotic transmission of this disease from animals to human are associated to climate change, unprotective husbandry practices, eating habits and social behavior of the concerned population. To control and eradicated this disease, different measures have to be taken including vaccination, maintaining farm hygiene, public education and environmental protection. The elimination of sero-reactors, development of control strategies, and education programs regarding the prevention and control of this zoonotic disease are highly needed in developing countries(AR et al. 2014 ). There is no documented information on how and when bovine brucellosis was introduced into Ethiopia. However, several serological study have been conducted in the last two decades and showed that it is endemic and widespread(Degefu et al., 2011 ). Pastoralists with high livestock population are known for their seasonal migration habit to search for feed and water during the dry period which results in intermingling of different herd groups and sometimes with wild animals at watering point and on the field. This results in transmission of disease like brucellosis from one herd to another and from domestic to wild animals. Because of this, periodic investigations needs to be implemented to enhance understanding about Brucella epidemiology which in turn is very important to refine control strategy (Muradrasoli et al., 2015 ). Therefore, this study was conducted to determine seroprevalence and to investigate potential risk factors of cattle brucellosis in Amibara district of Afar region. Materials And Methods Description of the study area This study was conducted in Amibara district of Gabirasu zone (Zone 3 of Afar region) located in the Middle Awash Valley of Ethiopia (Figure 1). Amibara district is about 270km to the North East of Addis Ababa and has 19 kebeles with total population of ~63,378, of which 35,374 were men and 28,004 women. The altitude of Amibara district is 740m above sea level. Fourteen years climatic data on monthly basis showed that the average maximum and minimum temperature of the area is 34°C and 19°C, respectively, and its annual total rainfall is about 571 mm (Chekol and Mnalku 2012). The livestock population of the Amibara district is composed of 103, 959 cattle, 122, 526 goats, 48,043 sheep, 3,888 donkeys and 39,995 camels (CSA 2007). Study Population In the present study the target study population were cattle owned by pastoralists. Only indigenous local breed of cattle with no history of vaccination and older than six months of age were recruited into the study. During sampling, cattle’s were classified into three age groups (5 years) as young ,adult and old respectively (Ibrahim et al . 2011). Study Design and sampl ing techniques A cross-sectional study design was employed from October 2019 to May 2020 in order to determine seroprevalence and associated risk factors of cattle brucellosis. Study kebeles were selected by simple random sampling technique. To select cattle herds in the proposed kebeles, purposive sampling technique was employed base accessibility and willingness of the herd owners to cooperate. Then each herd were stratified into subgroup based on age and sex to ensure equal representation of all subgroup. From each subgroup, individual animals were selected by systematic sampling technique and information related to environmental and study animals were also accessed. The sample size for serological study was calculated by Thrusfield formula using previous study result by(Asgedom et al . 2016) who reported 2.4% in Alage district . However, in order to increase precision and reduce standard error, the minimum sample size obtained by calculation was increased by four-fold and 181 cattle were sampled. Blood sample collection After disinfecting the site of jugular vein, about 8ml of blood sample was collected into sterile plain vacutainer tube and labeled with code describing each study animal. Then; the samples were taken to Werer Agricultural Research center, animal health research laboratory and kept overnight at room temperature to separate the serum and the clotted red blood cells according to (OIE 2018). Then the serum was gently decanted into sterile cryovials (1.8ml), labeled and stored at -20 0 c Laboratory Diagnosis A) Rose Bengal Plate Test (RBPT) All serum samples were screened using RBPT at National Veterinary Institute according to the procedure described by the World Organization for Animal Health (OIE 2018). In the laboratory, serum samples were kept at room temperature for 30 minutes and then, screened for anti-Brucella antibodies using commercial kits of the standard Rose Bengal Plate Test (RBPT). B. abortus antigens (Lillidale Diagnostics, Holt wimborne, Dorset, BH21 7DG, United Kingdom) and their positive and negative control sera were used. The detailed procedures for the RBPT were that, 30μl of cattle serum and 30μl of antigen was mixed on a test plate and rocked for 4 minutes. After four minutes of rocking, visible agglutination was considered as positive. Agglutinations were recorded as 0, +, ++ and +++, according to the degree of agglutination (Nielsen 2002). A score of 0 indicates the absence of agglutination; + indicates barely visible agglutination; ++ indicates fine agglutination, and +++ indicates coarse clumping. The presence of agglutination at any degree was considered as positive reaction while the absence of agglutination was considered as negative. B) Complement Fixation Test (CFT) Serum samples that reacted positively to RBPT were further retested by CFT for confirmation using standard B. abortus antigen S99 (New Haw, Addlestone, Surrey, KTI5, 3NB-UK). Preparation of the reagent is evaluated by titration and was performed according to protocols recommended by World Organization for Animal Health(OIE 2018). Sera with strong reaction, more than 75% fixation of complement (3+) at a dilution of 1:5 or at least with 50% fixation of complement (2+) at a dilution of 1:10 and above was considered as positive and lack of fixation/complete hemolysis was considered as negative result. Only samples that gave signals for both RBPT and CFT were considered positive since no single test is appropriate in all epidemiological situations due to problems of sensitivity and or specificity of the tests as recommended by OIE and other reports(Tumwine et al . 2015). Data Analysis Risk factors and serological results were recorded into microsoft Excel® Spread Sheet and analysis was done using R-Software version 4.0.3. Prevalence was calculated by dividing the number of positive animals to the total number of animals tested. Fisher’s exact test was used to calculate associations of risk factors with brucella seropositivity. Since the number of outcomes of interest is less than 10%, firth’s bias reduced logistic regression model was used to measure the association of potential risk factors with brucella seropositivity (Puhr et al . 2017). Odds ratio (OR) was used to indicate the relationship between risk factors with animal level seroprevalence of brucellosis. P-value less than 0.05 were considered statistically significant in all analysis. Ethical consideration Ethical approval for collection and analysis of animal materials was offered by animal research ethical review committee of the College of Veterinary Medicine and Agriculture (CVMA) with certificate ref. number of VM/ERC/03/01/12/2020. Results Result of brucellosis diagnosis in cattle and associated risk factors In this study, the sero-prevalence of brucella infection at individual animal level was computed by RBPT and CFT. Among 181 serum samples tested, 10.5% (95% CI:0.06-0.15) were found reactive for brucella infection by RBPT whereas 2.2% (95% CI:0.00-0.04) of them were confirmed to be brucella seropositive by CFT. Table 1 : Joint animal and herd level prevalence of cattle brucellosis in Amibara district of Afar Region Herd size Individual animals Herd Level No tested RBPT Positive (%) CFT positive (%) No tested RBPT Positive (%) CFT Positive (%) Small 45 3(6.67) 1(2.22) 8 3(37.5) 1(12.5) Medium 46 2(4.35) 0(0.00) 4 2(50) 0(0.00) Large 90 14(15.56) 3(3.33) 7 6(85.7) 2(28.57) In the present study, abortion was considered as an important risk factor and showed statistically significant association with brucella seropositivity in cattle p≤0.002 * (Table 2) Table 2: Seroprevalence and potential risk factors of brucellosis in cattle Variables N o tested Seropositive Prevalence (%) Fisher exact Test value P-value Sex 1.948 a 0.259 Male 13 1 7.7 Female 168 3 1.78 Age 6.77 b 0.021 * Young 30 0 0 Adult 123 2 1.62 Old 28 2 7.14 Body condition 1.462 b 0.481 Poor 46 0 0 Medium 107 3 2.8 Good 28 1 3.57 Herd size 0.364 b 1 50 47 1 2.13 Number of Parity ** 9.433 b 0.004 * 0 25 0 0% 1-5 117 1 0.85 >5 26 2 7.69 Abortion History ** 16.02 b 0.002 * Recent Abortion 21 3 14.3 No Abortion 147 0 0% P. retention ** 19.1 b 0.003 Yes 4 2 50 No 162 1 0.167 N o of test: Total number of animals tested; b -Fishers exact test value, ꭓ 2 : Chi-square, a- Chi- square value, * Significant; ** Male animals were excluded from the total number of cattle Based on fisher exact test, different host risk factors were considered and age of the animals (2 b =6.77, P≤0.02), number of parity (ꭓ 2b =9.433, P≤0.004), abortion history (ꭓ 2b =16.02, P≤0.002) and placental retention (ꭓ 2b =19.1, P≤0.01) were found significantly associated with brucellosis seropositivity in cattle (Table 2). However, sex and body condition of the study animals did not show statistically significant association brucella seropositivity(P>0.05). Firth bias reduced logistic regression analysis of factors associated with Brucella Seropositivity cattle After computing firth’s bias reduced logistic regression, multiparous animals (>5) and placental retention showed statistically significant association with brucella infection in cattle (Table 3). It was also indicated that, adult animals were 16.78 times more at risk of contracting brucellosis when compared with young one even though it doesn’t stand statistically significant. In addition, multiparous animals were 10.68 times and animals with history of placental retention were 72.72 times more due brucella infection when compared with bovine having zero parity and those with no history of placental retention respectively (Table 3). Table 3 : Firth bias reduced logistic regression analysis of factors associated with Brucellosis Seropositivity in cattle by CFT. Variables Number tested Seropositive (N, %) OR (95%CI) P-value Age Young 25 0 1 1 - Adult 117 1 16.78 (-0.235,7.86) 0.0695 Old 26 2 0.122 (-8.586,3.93) 0.446 Parity number Null 25 0 1 1 - 1-5 116 0 0.007 (-10.8, -1.65) 0.181 Greater than 5 27 3 10.68 (-1.19,7.595) 0.0042 Placental retention Yes 6 2 72.72 (1.46,9.272) 0.0026 No 173 2 1 1 - Discussion In the present study, 10.5% of tested cattle were found reactive for brucella infection by RBPT among which 2.2% were confirmed to be brucella seropositive by CFT. The present study finding is in close agreement with the earlier finding of (Yimer 2017 ) in Amibara, (Asmare et al. 2007 ) in Sidama, (Alemu et al. 2014 ) in East shoa zone, (Kassahun 2004 ) in Sidama and (Jergefa et al. 2009 ) in central Oromia who reported 2.4%, 2.46%, 2.0%, 2.5% and 2.9% respectively. However, it is lower when compared to the findings of (Megersa et al. 2011b ) who reported 10.6% in Borana zone, (Mekonnen et al. 2010 ) who reported 4.9% in Western Tigray, (Tschopp et al. 2015 ) who reported 4.8% in pastoral community of Afar and Oromia region adjacent to Awash national park and (Dinka and Chala 2009 ) who reported 15.2% in East Showa zone of Oromia region . The seroprevalence result of the current study is also lower than some reports in other African countries. For instance, a prevalence of 3.4% in Cameroon by (Awah-Ndukum et al. 2018 ), 24.5% in Sudan by (Angara et al. 2004 ), 12–15.8% in Uganda by ( Miller et al. 2015) and 26.50 % in India by (Chand and Chhabra 2013 ) were relatively higher than the present finding. The difference in brucellosis seroprevalence of the current and previous study results might be due to variation in sample size, husbandry and management practices, virulence of the organisms and diagnostic test employed. In addition to estimation of seroprevalence, this study was also carried out aiming to assess the risk factors associated with disease occurrence in cattle. Therefore; sex, age, body condition, number of parity and herd size of the cattle and history of placental retention were accessed. As a result, some of the considered risk factors were not significantly associated with brucellosis seropositivity in cattle except age, number of parities, abortion history and placental retention (Table 2 ). The insignificant association of sex with brucellosis seropositivity disagree with findings of (Dinka and Chala 2009 ; Kebede et al. 2008 ) who reported higher cases of brucellosis in female animals than in males. This might be due to the incomparable number of male cattle with female animals in the present study. Concerned with body condition score, 3.57% of animals with good body condition were found positive whereas no reactor animals encountered from those with poor body condition. This finding is in agreement with the report of (Awah-Ndukum et al. 2018 ), who stated insignificant association of body condition score with susceptibility to brucellosis. Herd size of the cattle was also considered as risk factors since brucellosis is a disease of herd importance. Accordingly, the herd level seroprevalence was 18.18% which is in close agreement with the finding of(Ibrahim et al. 2010 ). Even though large herd sizes are mor prone to brucella infection, no significant association of herd size with brucellosis seropositivity was observed in the current study(P>0.05). Consequently, this finding disagrees with findings of(Tolosa 2004 ). After computing firth bias reduced logistic regression analysis, only multiparous and animals with history of placental retention showed statistically significant association with brucella infection(p<0.05). This finding is in close agreement with (Alemu et al., 2014 )and (Isloor et al., 1998 ) who reported significant association of parity and placental retention with brucella infection respectively. It was also appreciated that; the owners usually keep their cattle in national park to look for water and pasture which allow close contact of domestic animals with jungle beast. Due to this, brucellosis can spread in either direction from contaminated pasture and water point or by direct contact of aborted fetal materials. Study conducted in Tanzania indicated, the interactions between wildlife and livestock as potential risk for brucellosis transmission to humans and livestock. In human, brucellosis is transmitted through consumption of unpasteurized dairy products or through direct contact with infected animals, placentas or aborted fetuses (Haileselassie et al., 2011 ). Evidences shown that, the social habit of raw milk and meat consumption, unsafe handling of aborted fetuses and placenta, assisting parturition, and occupations related to animal contacts have been reported to be some important epidemiological factors for human brucellosis(Dinka and Chala 2009 ; Haileselassie et al. 2011 ). Study conduct by (Eshetu et al. 2018 ) indicated, brucellosis were 5.11 times more in those who had assist animals during parturition compared to those who did not. This stands true for pastoral society whom believed to lead mobile lifestyle with few accesses to veterinary service. So, this condition creates a favorable condition for widespread and permanent occurrence of brucellosis in the area. Conclusion Brucellosis is a chronic bacterial disease of domestic and wild mammals having worldwide distribution. Cattle contract brucellosis through direct and indirect contact with infected animals and their excreta, ingestion of infected materials and sometimes aerosol transmission is expected. Based on the nature of the disease and ease of transmission, brucellosis is common in the pastoral society of Afar region due to their habit of mobile life style which allows intermingling of livestock and consumption of raw milk in human. Generally, the current study provides baseline data on cattle’s brucellosis in the current study district of Afar region, Ethiopia. Hence, working to enhance the awareness level of the pastoral society about the public health and economic significance of brucellosis through training and subsequent isolation and characterization of circulating strain and biotype is very important. References Alemu F, Admasu P, Feyera T, Niguse A (2014) "Seroprevalence of bovine brucellosis in eastern Showa, Ethiopia". Acad J Anim Dis 3:27–32 Angara TEE, Ism ail AA, Agab H, Saeed NS (2004) "Sero-prevalence of bovine brucellosis in Kuku Dairy Scheme. Khartoum North, Sudan", pp 0 0 AR B, Adehossi SCE, Matthys F, Vias GF, Yenikoye a E, Thys (2014) " La brucellose en Afrique Subsaharienne. La Brucell. en Afrique Subsaharienne". Ann Méd Vét 158:139–159 Asgedom H, Damena D, Duguma R, (2016), "Seroprevalence of bovine brucellosis and associated risk factors in and around Alage district, Ethiopia", Springerplus. 5,851 Asmare K, Prasad S, Asfaw Y, Gelaye E, Ayelet G, Zeleke A (2007) "Seroprevalence of brucellosis in cattle and in high risk animal health professionals in Sidama Zone, Southern Ethiopia. Ethiopia" Vet J 11:59–68 Awah-Ndukum J, Mouiche MMM, Kouonmo-Ngnoyum L, Bayang HN, Manchang TK, Poueme RSN, Kouamo J, Ngu-Ngwa V, Assana E, Feussom KJM (2018) Seroprevalence and risk factors of brucellosis among slaughtered indigenous cattle, abattoir personnel and pregnant women in Ngaoundéré, Cameroon". BMC Infect Dis 18:611 Chand P, Chhabra R (2013) "Herd and individual animal prevalence of bovine brucellosis with associated risk factors on dairy farms in Haryana and Punjab in India". Trop Anim Health Prod 45:1313–1319 Chekol W, Mnalku A (2012) "Selected physical and chemical characteristics of soils of the middle awash irrigated farm lands, Ethiopia". Ethiop J Agric Sci 22:127–142 Corbel J (2006) "Brucellosis in humans and animals". WHO Press, Switz CSA J (2007) "Agricultural Sample Survey 2006-07". Vol. I, Addis Ababa Degefu H, Mohamud M, Hailemelekot M, Yohannes M, (2011);"Seroprevalence of bovine brucellosis in agro pastoral areas of Jijjiga zone of Somali National Regional State, Eastern Ethiopia", Ethiop. Vet. J 15 Deka RP, Magnusson U, Grace D, Lindahl J, Pratim R, Magnusson U, Grace D, Lindahl J,(2019), " Bovine brucellosis: prevalence, risk factors, economic cost and control options with particular reference to India- a review", Infect. Ecol. Epidemiol 9 Dinka H, Chala R (2009) "Seroprevalence Study of Bovine Brucellosis in Pastoral and Agro-Pastoral Areas of East Showa Zone, Oromia Regional State, Ethiopia", 6,508-512 Edao BM, Hailegebreal G, Berg S, Zewude A, Zeleke Y, Sori T, Almaw G, Whatmore AM, Ameni G, Wood JLN (2018) ),"Brucellosis in the Addis Ababa dairy cattle: the myths and the realities". BMC Vet Res 14:396 Eshetu A, Belina D, Jafer M, Mengistu S (2018) Sero-Prevalence Of Brucellosis In Camels And Febrile Human. Patients Attending Health Facilities In Selected Districts Of Eastern Ethiopia Haileselassie M, Kalayou S, Kyule M, Asfaha M, Belihu K, (2011), "Effect of Brucella infection on reproduction conditions of female breeding cattle and its public health significance in Western Tigray, northern Ethiopia", Vet. Med. Int. Ibrahim K, Thomas R, Peter K, Omer RA (2011) "A molecular survey on cystic echinococcosis in Sinnar area, Blue Nile state (Sudan)". Chin Med J (Engl 124:2829–2833 Ibrahim N, Belihu K, Lobago F, And MB-TAH, 2010, U., (2010), " Sero-prevalence of bovine brucellosis and its risk factors in Jimma zone of Oromia Region, South-western Ethiopia", Tropical Animal Health and Production 42,35-40 Isloor S, Renukaradhya GJ, Rajasekhar M (1998) A serological survey of bovine brucellosis in India. Rev Sci Tech Int des épizooties 17:781–785 Jergefa T, Kelay B, Bekana M, Teshale S, Gustafson H, Kindahl H, (2009), " Epidemiological study of bovine brucellosis in three agro-ecological areas of central Oromiya, Ethiopia", Rev. Sci. Tech 28–933 Kassahun A, (2004), " Epidemiology of bovine brucellosis in cattle and its seroprevalence in animal health professionals in Sidama Zone, Southern Ethiopia" Master Sci. thesis. Addis Ababa Univ. Fac. Vet. Med. Debre Zeit, Ethiop. Kebede T, Ejeta G, Ameni G (2008) " Seroprevalence of bovine brucellosis in smallholder farms in central Ethiopia (Wuchale-Jida district)". Rev Med Vet (Toulouse) 159:3 Lakew A, Hiko A, Abraha A, Hailu SM, (2019), " Sero-prevalence and community awareness on the risks associated with Livestock and Human brucellosis in selected districts of Fafan Zone of Ethiopian-Somali National Regional State", Vet. Anim. Sci. Massis F, De, Zilli K, Di G, Id D, Nuvoloni R, Pelini S, Sacchini L, Alterio ND, Giannatale E, Di, (2019), " Distribution of Brucella field strains isolated from livestock, wildlife populations, and humans in Italy from 2007 to 2015", Plose one 1–16 Mathew C, Stokstad M, Johansen TB, Klevar S, Mdegela RH, Mwamengele G, Michel P, Escobar L, Fretin D, Godfroid J (2015) "First isolation, identification, phenotypic and genotypic characterization of Brucella abortus biovar 3 from dairy cattle in Tanzania". BMC Vet Res 11:1–7 Megersa B, Biffa D, Niguse F, Rufael T, Asmare K, Skjerve E (2011b) "Cattle brucellosis in traditional livestock husbandry practice in Southern and Eastern Ethiopia, and its zoonotic implication". Acta Vet Scand 53:24 Mekonnen H, Kalayou S, Kyule M (2010) " Serological survey of bovine brucellosis in barka and arado breeds (Bos indicus) of Western Tigray, Ethiopia". Prev Vet Med 94:28–35 Muradrasoli S, Mugizi DR, Waiswa C, Erume J, Boqvist S, Mboowa G, Magnusson U, Nasinyama GW, Klint M,(2015), "Isolation and Molecular Characterization of Brucella Isolates in Cattle Milk in Uganda", Biomed Res. Int 2015, 1-9 Nielsen K (2002) " Diagnosis of brucellosis by serology". Vet Microbiol 90:447–459 OIE, (2018), " Brucellosis(B.abortus, B. melitensis, and B. suis)"358 OIE A, (2008), " Manual of diagnostic tests and vaccines for terrestrial animals", Off. Int. des Epizoot. paris, Fr 1092–1106 Puhr R et al (2017) "Firth's logistic regression with rare events. accurate effect estimates and predictions", Statistics in medicine 36,2302-2317 Tibesso G, Ibrahim N, Tolosa T (2014) " Sero-prevalence of bovine and human brucellosis in Adami Tulu, central Ethiopia". World Appl Sci J 31:776–780 Tolosa T (2004) Seroprevalence study of bovine brucellosis and its public health significance in selected sites of Jimma Zone, Western Ethiopia. Ethiop. Msc Thesis, Addis Ababa Univ. Fac. Vet. Med. Debre Zeit Tschopp R, Bekele S, Moti T, Young D, Aseffa A (2015) " Brucellosis and bovine tuberculosis prevalence in livestock from pastoralist communities adjacent to Awash National Park, Ethiopia". Prev Vet Med 120:187–194 Tumwine G, Matovu E, Kabasa JD, Owiny DO, Majalija S (2015) "Human brucellosis: sero-prevalence and associated risk factors in agro-pastoral communities of Kiboga District, Central Uganda". BMC Public Health 15:900 Yimer G, (2017), " Seroprevalence and isolation of brucella species from camel and cattle with history af recent abortion in Amibara District, Afar Regional state", Msc Thesis. Declarations The authors declare that there is no competing interest. 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Bovine brucellosis is the most common but under reported bacterial diseases which is known to creates a serious socio-economic problem in both intensive and extensive livestock production systems(Deka et al., \u003cspan citationid=\"CR12\" class=\"CitationRef\"\u003e2019\u003c/span\u003e). It is caused by Brucella \u003cem\u003espp\u003c/em\u003e and manifests itself as abortion and infertility in domestic and wild animal species and reduced milk production (Mathew et al., \u003cspan citationid=\"CR25\" class=\"CitationRef\"\u003e2015\u003c/span\u003e). In cattle the disease is mainly caused by \u003cem\u003eB. abortus\u003c/em\u003e and characterized by inflammation of the genitals and foetal membranes, abortions, sterility and lesions in the lymphatic system and joints(Corbel, \u003cspan citationid=\"CR9\" class=\"CitationRef\"\u003e2006\u003c/span\u003e).\u003c/p\u003e \u003cp\u003eDeveloping countries with limited resources have not yet fully launched programs featuring any aspects of brucellosis intervention, since they are facing other priority diseases that are more spectacular (Lakew \u003cem\u003eet al\u003c/em\u003e.,2019). Risk factors associated with brucellosis can be categorized into management, animal, and environmental factors(AR et al., \u003cspan citationid=\"CR3\" class=\"CitationRef\"\u003e2014\u003c/span\u003e). Sources of infection for the transmission of the bovine brucellosis are aborted fetuses, retained fetal membranes, and vaginal discharges and milk from infected animals(Edao et al., \u003cspan citationid=\"CR14\" class=\"CitationRef\"\u003e2018\u003c/span\u003e). In human, brucellosis is one of the most common bacterial zoonotic infections, but remains under reported disease in Ethiopia due to the absence of diagnostic facility in public hospitals. \u003cem\u003eB\u003c/em\u003e. \u003cem\u003emelitensis\u003c/em\u003e, \u003cem\u003eB\u003c/em\u003e. \u003cem\u003eabortus\u003c/em\u003e, and \u003cem\u003eB\u003c/em\u003e. \u003cem\u003esuis\u003c/em\u003e are known to induce significant public health problems (Massis et al., \u003cspan citationid=\"CR24\" class=\"CitationRef\"\u003e2019\u003c/span\u003e).\u003c/p\u003e \u003cp\u003eThe risks of zoonotic transmission of this disease from animals to human are associated to climate change, unprotective husbandry practices, eating habits and social behavior of the concerned population. To control and eradicated this disease, different measures have to be taken including vaccination, maintaining farm hygiene, public education and environmental protection. The elimination of sero-reactors, development of control strategies, and education programs regarding the prevention and control of this zoonotic disease are highly needed in developing countries(AR et al. \u003cspan citationid=\"CR3\" class=\"CitationRef\"\u003e2014\u003c/span\u003e). There is no documented information on how and when bovine brucellosis was introduced into Ethiopia. However, several serological study have been conducted in the last two decades and showed that it is endemic and widespread(Degefu et al., \u003cspan citationid=\"CR11\" class=\"CitationRef\"\u003e2011\u003c/span\u003e). Pastoralists with high livestock population are known for their seasonal migration habit to search for feed and water during the dry period which results in intermingling of different herd groups and sometimes with wild animals at watering point and on the field. This results in transmission of disease like brucellosis from one herd to another and from domestic to wild animals. Because of this, periodic investigations needs to be implemented to enhance understanding about \u003cem\u003eBrucella\u003c/em\u003e epidemiology which in turn is very important to refine control strategy (Muradrasoli et al., \u003cspan citationid=\"CR28\" class=\"CitationRef\"\u003e2015\u003c/span\u003e). Therefore, this study was conducted to determine seroprevalence and to investigate potential risk factors of cattle brucellosis in Amibara district of Afar region.\u003c/p\u003e"},{"header":"Materials And Methods","content":"\u003cp\u003e\u003cstrong\u003eDescription of the study area\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003e\u0026nbsp;\u003c/p\u003e\n\u003cp\u003eThis study was conducted in Amibara district of Gabirasu zone (Zone 3 of Afar region) located in the Middle Awash Valley of Ethiopia (Figure 1). Amibara district is about 270km to the North East of Addis Ababa and has 19 kebeles with total population of ~63,378, of which 35,374 were men and 28,004 women. The altitude of Amibara district is 740m above sea level. Fourteen years climatic data on monthly basis showed that the average maximum and minimum temperature of the area is 34\u0026deg;C and 19\u0026deg;C, respectively, and its annual total rainfall is about 571 mm (Chekol and Mnalku 2012). The livestock population of the Amibara district is composed of 103, 959 cattle, 122, 526 goats, 48,043 sheep, 3,888 donkeys and 39,995 camels (CSA 2007).\u003c/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c/p\u003e\n\u003cp id=\"isPasted\"\u003e\u003cstrong\u003eStudy Population\u0026nbsp;\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003e\u0026nbsp;\u003c/p\u003e\n\u003cp\u003eIn the present study the target study population were cattle owned by pastoralists. Only indigenous local breed of cattle with no history of vaccination and older than six months of age were recruited into the study. During sampling, cattle\u0026rsquo;s were classified into three age groups (\u0026lt;2 years, 2-5years and \u0026gt;5 years) as young ,adult and old respectively\u0026nbsp;(Ibrahim\u003cem\u003e\u0026nbsp;et al\u003c/em\u003e. 2011).\u0026nbsp;\u003c/p\u003e\n\u003cp\u003e\u0026nbsp;\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eStudy Design and sampl\u003c/strong\u003e\u003cstrong\u003eing techniques\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u0026nbsp;\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eA cross-sectional study design was employed\u0026nbsp;from October 2019 to May 2020\u0026nbsp;in order to determine seroprevalence and associated risk factors of cattle brucellosis.\u0026nbsp;Study kebeles were selected by simple random sampling technique. To select cattle herds in the proposed kebeles, purposive sampling technique was employed base accessibility and willingness of the herd owners to cooperate. Then each herd were stratified into subgroup based on age and sex to ensure equal representation of all subgroup. From each subgroup, individual animals were selected by systematic sampling technique and\u0026nbsp;information related to environmental and study animals were also accessed. The sample size for serological study was calculated by Thrusfield formula using previous study result by(Asgedom \u003cem\u003eet al\u003c/em\u003e. 2016)\u0026nbsp;who reported 2.4% in Alage district .\u0026nbsp;However, in order to increase precision and reduce standard error, the minimum sample size obtained by calculation was increased by four-fold and 181 cattle were sampled.\u0026nbsp;\u003c/p\u003e\n\u003cp\u003e\u0026nbsp;\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eBlood sample collection\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003e\u003cem\u003e\u0026nbsp;\u003c/em\u003e\u003c/p\u003e\n\u003cp\u003eAfter disinfecting the site of jugular vein, about 8ml of blood sample was collected into sterile plain vacutainer tube and labeled with code describing each study animal. Then; the samples were taken to Werer Agricultural Research center, animal health research laboratory and\u0026nbsp;kept overnight at room temperature to separate the serum and the clotted red blood cells according to\u0026nbsp;(OIE 2018). Then the serum was gently decanted into sterile cryovials (1.8ml),\u0026nbsp;labeled and stored at -20\u003csup\u003e0\u003c/sup\u003ec\u0026nbsp;\u003c/p\u003e\n\u003cp\u003e\u0026nbsp;\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eLaboratory Diagnosis\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003e\u0026nbsp;\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eA) Rose Bengal Plate Test (RBPT)\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003e\u0026nbsp;\u003c/p\u003e\n\u003cp\u003eAll serum samples were screened using RBPT at National Veterinary Institute according to the procedure described by the World Organization for Animal Health\u0026nbsp;(OIE \u0026nbsp;2018). In the laboratory, serum samples were kept at room temperature for 30 minutes and then, screened for anti-Brucella antibodies using commercial kits of the standard Rose Bengal Plate Test (RBPT). \u003cem\u003eB. abortus\u003c/em\u003e antigens (Lillidale Diagnostics, Holt wimborne, Dorset, BH21 7DG, United Kingdom) and their positive and negative control sera were used. The detailed procedures for the RBPT were that, 30\u0026mu;l of cattle serum and 30\u0026mu;l of antigen was mixed on a test plate and rocked for 4 minutes. After four minutes of rocking, visible agglutination was considered as positive. Agglutinations were recorded as 0, +, ++ and +++, according to the degree of agglutination\u0026nbsp;(Nielsen \u0026nbsp;2002). A score of 0 indicates the absence of agglutination; + indicates barely visible agglutination; ++ indicates fine agglutination, and +++ indicates coarse clumping. The presence of agglutination at any degree was considered as positive reaction while the absence of agglutination was considered as negative.\u0026nbsp;\u003c/p\u003e\n\u003cp\u003e\u0026nbsp;\u003c/p\u003e\n\u003ch4\u003e\u003cstrong\u003eB) Complement Fixation Test (CFT)\u003c/strong\u003e\u003c/h4\u003e\n\u003cp\u003e\u0026nbsp;\u003c/p\u003e\n\u003cp\u003eSerum samples that reacted positively to RBPT were further retested by CFT for confirmation using standard \u003cem\u003eB. abortus\u003c/em\u003e antigen S99 (New Haw, Addlestone, Surrey, KTI5, 3NB-UK).\u0026nbsp;Preparation of the reagent is evaluated by titration and was performed according to protocols recommended by World Organization for Animal Health(OIE 2018). Sera with strong reaction, more than 75% fixation of complement (3+) at a dilution of 1:5 or at least with 50% fixation of complement (2+) at a dilution of 1:10 and above was considered as positive and lack of fixation/complete hemolysis was considered as negative result. Only samples that gave signals for both RBPT and CFT were considered positive since no single test is appropriate in all epidemiological situations due to problems of sensitivity and or specificity of the tests as recommended by OIE and other reports(Tumwine \u003cem\u003eet al\u003c/em\u003e. 2015).\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u0026nbsp;\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eData Analysis\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003e\u0026nbsp;\u003c/p\u003e\n\u003cp\u003eRisk factors and serological results were recorded into microsoft Excel\u0026reg; Spread Sheet and analysis was done using R-Software version 4.0.3. Prevalence was calculated by dividing the number of positive animals to the total number of animals tested. Fisher\u0026rsquo;s exact test was used to calculate associations of risk factors with brucella seropositivity. Since the number of outcomes of interest is less than 10%, firth\u0026rsquo;s bias reduced logistic regression model was used to measure the association of potential risk factors with brucella seropositivity (Puhr \u003cem\u003eet al\u003c/em\u003e. 2017). Odds ratio (OR) was used to indicate the relationship between risk factors with animal level seroprevalence of brucellosis. P-value less than 0.05 were considered statistically significant in all analysis.\u0026nbsp;\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eEthical consideration\u003c/strong\u003e\u003cstrong\u003e\u0026nbsp;\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003e\u0026nbsp;\u003c/p\u003e\n\u003cp\u003eEthical approval for collection and analysis of animal materials was offered by animal research ethical review committee of the College of Veterinary Medicine and Agriculture (CVMA) with certificate ref. number of VM/ERC/03/01/12/2020.\u0026nbsp;\u003c/p\u003e"},{"header":"Results","content":"\u003cp\u003e\u003cstrong\u003eResult of brucellosis diagnosis in cattle and associated risk factors\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u0026nbsp;\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eIn this study, the sero-prevalence of \u003cem\u003ebrucella\u0026nbsp;\u003c/em\u003einfection at individual animal level was computed by RBPT and CFT. Among 181 serum samples tested, 10.5% (95% CI:0.06-0.15) were found reactive for brucella infection by RBPT whereas 2.2% (95% CI:0.00-0.04) of them were confirmed to be brucella seropositive by CFT.\u003c/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c/p\u003e\n\u003cp id=\"isPasted\" style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:150%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cstrong\u003e\u003cspan style='font-size:16px;line-height:150%;font-family:\"Times New Roman\",serif;'\u003eTable 1\u003c/span\u003e\u003c/strong\u003e\u003cspan style='font-size:16px;line-height:150%;font-family:\"Times New Roman\",serif;'\u003e: Joint animal and herd level prevalence of cattle brucellosis in Amibara district of Afar Region\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n\u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:150%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cspan style='font-size:16px;line-height:150%;font-family:\"Times New Roman\",serif;'\u003e\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n\u003ctable style=\"width:467.75pt;border-collapse:collapse;border:none;\"\u003e\n \u003ctbody\u003e\n \u003ctr\u003e\n \u003ctd style=\"width: 63pt;border-color: black currentcolor;border-style: solid none;border-width: 1pt medium;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height: 150%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cstrong\u003e\u003cspan style='font-size:16px;line-height:150%;font-family:\"Times New Roman\",serif;color:black;'\u003eHerd size\u0026nbsp;\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd colspan=\"3\" style=\"width: 193.25pt;border-color: black currentcolor;border-style: solid none;border-width: 1pt medium;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:150%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:center;'\u003e\u003cstrong\u003e\u003cspan style='font-size:16px;line-height:150%;font-family:\"Times New Roman\",serif;color:black;'\u003eIndividual animals\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd colspan=\"3\" style=\"width: 211.5pt;border-color: black currentcolor;border-style: solid none;border-width: 1pt medium;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:150%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:center;'\u003e\u003cstrong\u003e\u003cspan style='font-size:16px;line-height:150%;font-family:\"Times New Roman\",serif;color:black;'\u003eHerd Level\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width: 63pt;border: medium none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height: 150%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cstrong\u003e\u003cspan style='font-size:16px;line-height:150%;font-family:\"Times New Roman\",serif;color:black;'\u003e\u0026nbsp;\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 40.25pt;border: medium none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height: 150%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cspan style='font-size:16px;line-height:150%;font-family:\"Times New Roman\",serif;color:black;'\u003eNo tested\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 76.5pt;border: medium none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height: 150%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cspan style='font-size:16px;line-height:150%;font-family:\"Times New Roman\",serif;color:black;'\u003eRBPT\u003c/span\u003e\u003c/p\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height: 150%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cspan style='font-size:16px;line-height:150%;font-family:\"Times New Roman\",serif;color:black;'\u003ePositive (%)\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 76.5pt;border: medium none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height: 150%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cspan style='font-size:16px;line-height:150%;font-family:\"Times New Roman\",serif;color:black;'\u003eCFT\u003c/span\u003e\u003c/p\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height: 150%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cspan style='font-size:16px;line-height:150%;font-family:\"Times New Roman\",serif;color:black;'\u003epositive (%)\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 0.75in;border: medium none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height: 150%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cspan style='font-size:16px;line-height:150%;font-family:\"Times New Roman\",serif;color:black;'\u003eNo tested\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 76.5pt;border: medium none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height: 150%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cspan style='font-size:16px;line-height:150%;font-family:\"Times New Roman\",serif;color:black;'\u003eRBPT\u003c/span\u003e\u003c/p\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height: 150%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cspan style='font-size:16px;line-height:150%;font-family:\"Times New Roman\",serif;color:black;'\u003ePositive (%)\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 81pt;border: medium none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height: 150%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cspan style='font-size:16px;line-height:150%;font-family:\"Times New Roman\",serif;color:black;'\u003eCFT\u003c/span\u003e\u003c/p\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height: 150%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cspan style='font-size:16px;line-height:150%;font-family:\"Times New Roman\",serif;color:black;'\u003ePositive (%)\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width: 63pt;border: medium none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height: 150%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cstrong\u003e\u003cspan style='font-size:16px;line-height:150%;font-family:\"Times New Roman\",serif;color:black;'\u003eSmall\u0026nbsp;\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 40.25pt;border: medium none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height: 150%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cspan style='font-size:16px;line-height:150%;font-family:\"Times New Roman\",serif;color:black;'\u003e45\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 76.5pt;border: medium none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height: 150%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cspan style='font-size:16px;line-height:150%;font-family:\"Times New Roman\",serif;color:black;'\u003e3(6.67)\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 76.5pt;border: medium none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height: 150%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cspan style='font-size:16px;line-height:150%;font-family:\"Times New Roman\",serif;color:black;'\u003e1(2.22)\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 0.75in;border: medium none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height: 150%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cspan style='font-size:16px;line-height:150%;font-family:\"Times New Roman\",serif;color:black;'\u003e8\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 76.5pt;border: medium none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height: 150%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cspan style='font-size:16px;line-height:150%;font-family:\"Times New Roman\",serif;color:black;'\u003e3(37.5)\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 81pt;border: medium none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height: 150%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cspan style='font-size:16px;line-height:150%;font-family:\"Times New Roman\",serif;color:black;'\u003e1(12.5)\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width: 63pt;border: medium none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height: 150%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cstrong\u003e\u003cspan style='font-size:16px;line-height:150%;font-family:\"Times New Roman\",serif;color:black;'\u003eMedium\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 40.25pt;border: medium none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height: 150%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cspan style='font-size:16px;line-height:150%;font-family:\"Times New Roman\",serif;color:black;'\u003e46\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 76.5pt;border: medium none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height: 150%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cspan style='font-size:16px;line-height:150%;font-family:\"Times New Roman\",serif;color:black;'\u003e2(4.35)\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 76.5pt;border: medium none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height: 150%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cspan style='font-size:16px;line-height:150%;font-family:\"Times New Roman\",serif;color:black;'\u003e0(0.00)\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 0.75in;border: medium none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height: 150%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cspan style='font-size:16px;line-height:150%;font-family:\"Times New Roman\",serif;color:black;'\u003e4\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 76.5pt;border: medium none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height: 150%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cspan style='font-size:16px;line-height:150%;font-family:\"Times New Roman\",serif;color:black;'\u003e2(50)\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 81pt;border: medium none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height: 150%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cspan style='font-size:16px;line-height:150%;font-family:\"Times New Roman\",serif;color:black;'\u003e0(0.00)\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width: 63pt;border-color: currentcolor currentcolor black;border-style: none none solid;border-width: medium medium 1pt;border-image: none 100% / 1 / 0 stretch;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height: 150%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cstrong\u003e\u003cspan style='font-size:16px;line-height:150%;font-family:\"Times New Roman\",serif;color:black;'\u003eLarge\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 40.25pt;border-color: currentcolor currentcolor black;border-style: none none solid;border-width: medium medium 1pt;border-image: none 100% / 1 / 0 stretch;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height: 150%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cspan style='font-size:16px;line-height:150%;font-family:\"Times New Roman\",serif;color:black;'\u003e90\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 76.5pt;border-color: currentcolor currentcolor black;border-style: none none solid;border-width: medium medium 1pt;border-image: none 100% / 1 / 0 stretch;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height: 150%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cspan style='font-size:16px;line-height:150%;font-family:\"Times New Roman\",serif;color:black;'\u003e14(15.56)\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 76.5pt;border-color: currentcolor currentcolor black;border-style: none none solid;border-width: medium medium 1pt;border-image: none 100% / 1 / 0 stretch;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height: 150%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cspan style='font-size:16px;line-height:150%;font-family:\"Times New Roman\",serif;color:black;'\u003e3(3.33)\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 0.75in;border-color: currentcolor currentcolor black;border-style: none none solid;border-width: medium medium 1pt;border-image: none 100% / 1 / 0 stretch;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height: 150%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cspan style='font-size:16px;line-height:150%;font-family:\"Times New Roman\",serif;color:black;'\u003e7\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 76.5pt;border-color: currentcolor currentcolor black;border-style: none none solid;border-width: medium medium 1pt;border-image: none 100% / 1 / 0 stretch;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height: 150%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cspan style='font-size:16px;line-height:150%;font-family:\"Times New Roman\",serif;color:black;'\u003e6(85.7)\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 81pt;border-color: currentcolor currentcolor black;border-style: none none solid;border-width: medium medium 1pt;border-image: none 100% / 1 / 0 stretch;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height: 150%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cspan style='font-size:16px;line-height:150%;font-family:\"Times New Roman\",serif;color:black;'\u003e2(28.57)\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003c/tbody\u003e\n\u003c/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c/p\u003e\n\u003cp\u003eIn the present study, abortion was considered as an important risk factor and showed statistically significant association with brucella seropositivity in cattle p\u0026le;0.002\u003csup id=\"isPasted\"\u003e*\u003c/sup\u003e(Table 2)\u003c/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c/p\u003e\n\u003cp id=\"isPasted\" style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:150%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cspan style='font-size:16px;line-height:150%;font-family:\"Times New Roman\",serif;color:black;'\u003eTable 2: Seroprevalence and potential risk factors of brucellosis in cattle\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n\u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:150%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cspan style='font-size:16px;line-height:150%;font-family:\"Times New Roman\",serif;color:black;'\u003e\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n\u003ctable style=\"width:472.25pt;border-collapse:collapse;border:none;\"\u003e\n \u003ctbody\u003e\n \u003ctr\u003e\n \u003ctd style=\"width: 112.25pt;border-color: black currentcolor;border-style: solid none;border-width: 1pt medium;padding: 0in 5.4pt;height: 27.85pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:150%;font-size:15px;font-family:\"Calibri\",sans-serif;'\u003e\u003cstrong\u003e\u003cspan style='font-size:16px;line-height:150%;font-family:\"Times New Roman\",serif;color:black;'\u003eVariables\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 63pt;border-color: black currentcolor;border-style: solid none;border-width: 1pt medium;padding: 0in 5.4pt;height: 27.85pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:150%;font-size:15px;font-family:\"Calibri\",sans-serif;'\u003e\u003cstrong\u003e\u003cspan style='font-size:16px;line-height:150%;font-family:\"Times New Roman\",serif;color:black;'\u003eN\u003cu\u003eo\u003c/u\u003e tested\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 58.5pt;border-color: black currentcolor;border-style: solid none;border-width: 1pt medium;padding: 0in 5.4pt;height: 27.85pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:150%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:center;'\u003e\u003cstrong\u003e\u003cspan style='font-size:16px;line-height:150%;font-family:\"Times New Roman\",serif;color:black;'\u003eSeropositive\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 85.5pt;border-color: black currentcolor;border-style: solid none;border-width: 1pt medium;padding: 0in 5.4pt;height: 27.85pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:150%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:center;'\u003e\u003cstrong\u003e\u003cspan style='font-size:16px;line-height:150%;font-family:\"Times New Roman\",serif;color:black;'\u003ePrevalence (%)\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 76.5pt;border-color: black currentcolor;border-style: solid none;border-width: 1pt medium;padding: 0in 5.4pt;height: 27.85pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:150%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:center;'\u003e\u003cstrong\u003e\u003cspan style='font-size:16px;line-height:150%;font-family:\"Times New Roman\",serif;color:black;'\u003eFisher exact\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:150%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:center;'\u003e\u003cstrong\u003e\u003cspan style='font-size:16px;line-height:150%;font-family:\"Times New Roman\",serif;color:black;'\u003eTest value\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 76.5pt;border-color: black currentcolor;border-style: solid none;border-width: 1pt medium;padding: 0in 5.4pt;height: 27.85pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:150%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:center;'\u003e\u003cstrong\u003e\u003cspan style='font-size:16px;line-height:150%;font-family:\"Times New Roman\",serif;color:black;'\u003eP-value\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width: 112.25pt;border: medium none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:150%;font-size:15px;font-family:\"Calibri\",sans-serif;'\u003e\u003cstrong\u003e\u003cem\u003e\u003cspan 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style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:150%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:center;'\u003e\u003cspan style='font-size:16px;line-height: 150%;font-family:\"Times New Roman\",serif;color:black;'\u003e\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width: 112.25pt;border: medium none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:150%;font-size:15px;font-family:\"Calibri\",sans-serif;'\u003e\u003cspan style='font-size:16px;line-height:150%;font-family:\"Times New Roman\",serif;color:black;'\u003eFemale\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 63pt;border: medium none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp 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style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:150%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:center;'\u003e\u003cspan style='font-size:16px;line-height:150%;font-family:\"Times New Roman\",serif;color:black;'\u003e\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width: 112.25pt;border: medium none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:150%;font-size:15px;font-family:\"Calibri\",sans-serif;'\u003e\u003cstrong\u003e\u003cem\u003e\u003cspan style='font-size:16px;line-height:150%;font-family:\"Times New Roman\",serif;color:black;'\u003eAge\u003c/span\u003e\u003c/em\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 63pt;border: medium none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:150%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:center;'\u003e\u003cspan style='font-size:16px;line-height: 150%;font-family:\"Times New Roman\",serif;color:black;'\u003e\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 58.5pt;border: medium none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:150%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:center;'\u003e\u003cspan style='font-size:16px;line-height: 150%;font-family:\"Times New Roman\",serif;color:black;'\u003e\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 85.5pt;border: medium none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:150%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:center;'\u003e\u003cspan style='font-size:16px;line-height: 150%;font-family:\"Times New Roman\",serif;color:black;'\u003e\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 76.5pt;border: medium none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:150%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:center;background:white;'\u003e\u003cspan style='font-size:16px;line-height:150%;font-family:\"Times New Roman\",serif;color:black;'\u003e6.77\u003csup\u003eb\u003c/sup\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 76.5pt;border: medium none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:150%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:center;background:white;'\u003e\u003cspan style='font-size:16px;line-height:150%;font-family:\"Times New Roman\",serif;color:black;'\u003e0.021\u003csup\u003e*\u003c/sup\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width: 112.25pt;border: medium none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:150%;font-size:15px;font-family:\"Calibri\",sans-serif;'\u003e\u003cspan style='font-size:16px;line-height:150%;font-family:\"Times New Roman\",serif;color:black;'\u003e\u0026nbsp;Young\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 63pt;border: medium none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp 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style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:150%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:center;'\u003e\u003cspan style='font-size:16px;line-height:150%;font-family:\"Times New Roman\",serif;color:black;'\u003e0\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 76.5pt;border: medium none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:150%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:center;'\u003e\u003cspan style='font-size:16px;line-height:150%;font-family:\"Times New Roman\",serif;color:black;'\u003e\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 76.5pt;border: medium none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:150%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:center;'\u003e\u003cspan style='font-size:16px;line-height:150%;font-family:\"Times New Roman\",serif;color:black;'\u003e\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width: 112.25pt;border: medium none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:150%;font-size:15px;font-family:\"Calibri\",sans-serif;'\u003e\u003cspan style='font-size:16px;line-height:150%;font-family:\"Times New Roman\",serif;color:black;'\u003e\u0026nbsp;Adult\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 63pt;border: medium none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp 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style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:150%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:center;'\u003e\u003cspan style='font-size:16px;line-height:150%;font-family:\"Times New Roman\",serif;color:black;'\u003e\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 76.5pt;border: medium none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:150%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:center;'\u003e\u003cspan style='font-size:16px;line-height:150%;font-family:\"Times New Roman\",serif;color:black;'\u003e\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width: 112.25pt;border: medium none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:150%;font-size:15px;font-family:\"Calibri\",sans-serif;'\u003e\u003cstrong\u003e\u003cem\u003e\u003cspan style='font-size:16px;line-height:150%;font-family:\"Times New Roman\",serif;color:black;'\u003eAbortion History\u003csup\u003e**\u003c/sup\u003e\u003c/span\u003e\u003c/em\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 63pt;border: medium none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:150%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:center;'\u003e\u003cspan style='font-size:16px;line-height: 150%;font-family:\"Times New Roman\",serif;color:black;'\u003e\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 58.5pt;border: medium none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:150%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:center;'\u003e\u003cspan style='font-size:16px;line-height: 150%;font-family:\"Times New Roman\",serif;color:black;'\u003e\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 85.5pt;border: medium none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:150%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:center;'\u003e\u003cspan style='font-size:16px;line-height: 150%;font-family:\"Times New Roman\",serif;color:black;'\u003e\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 76.5pt;border: medium none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:150%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:center;background:white;'\u003e\u003cspan style='font-size:16px;line-height:150%;font-family:\"Times New Roman\",serif;color:black;'\u003e16.02\u003csup\u003eb\u003c/sup\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 76.5pt;border: medium none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:150%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:center;background:white;'\u003e\u003cspan style='font-size:16px;line-height:150%;font-family:\"Times New Roman\",serif;color:black;border:none windowtext 1.0pt;padding:0in;'\u003e0.002\u003csup\u003e*\u003c/sup\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width: 112.25pt;border: medium none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:150%;font-size:15px;font-family:\"Calibri\",sans-serif;'\u003e\u003cspan style='font-size:16px;line-height:150%;font-family:\"Times New Roman\",serif;color:black;'\u003eRecent Abortion\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 63pt;border: medium none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:150%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:center;'\u003e\u003cspan style='font-size:16px;line-height:150%;font-family:\"Times New Roman\",serif;color:black;'\u003e21\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 58.5pt;border: medium none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:150%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:center;'\u003e\u003cspan style='font-size:16px;line-height:150%;font-family:\"Times New Roman\",serif;color:black;'\u003e3\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 85.5pt;border: medium none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:150%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:center;'\u003e\u003cspan style='font-size:16px;line-height:150%;font-family:\"Times New Roman\",serif;color:black;'\u003e14.3\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 76.5pt;border: medium none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:150%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:center;'\u003e\u003cspan style='font-size:16px;line-height:150%;font-family:\"Times New Roman\",serif;color:black;'\u003e\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 76.5pt;border: medium none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:150%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:center;'\u003e\u003cspan style='font-size:16px;line-height:150%;font-family:\"Times New Roman\",serif;color:black;'\u003e\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width: 112.25pt;border: medium none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:150%;font-size:15px;font-family:\"Calibri\",sans-serif;'\u003e\u003cspan style='font-size:16px;line-height:150%;font-family:\"Times New Roman\",serif;color:black;'\u003eNo Abortion\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 63pt;border: medium none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:150%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:center;'\u003e\u003cspan style='font-size:16px;line-height: 150%;font-family:\"Times New Roman\",serif;color:black;'\u003e147\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 58.5pt;border: medium none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:150%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:center;'\u003e\u003cspan style='font-size:16px;line-height: 150%;font-family:\"Times New Roman\",serif;color:black;'\u003e0\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 85.5pt;border: medium none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:150%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:center;'\u003e\u003cspan style='font-size:16px;line-height: 150%;font-family:\"Times New Roman\",serif;color:black;'\u003e0%\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 76.5pt;border: medium none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:150%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:center;'\u003e\u003cspan style='font-size:16px;line-height: 150%;font-family:\"Times New Roman\",serif;color:black;'\u003e\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 76.5pt;border: medium none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:150%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:center;'\u003e\u003cspan style='font-size:16px;line-height: 150%;font-family:\"Times New Roman\",serif;color:black;'\u003e\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width: 112.25pt;border: medium none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:150%;font-size:15px;font-family:\"Calibri\",sans-serif;'\u003e\u003cstrong\u003e\u003cem\u003e\u003cspan style='font-size:16px;line-height:150%;font-family:\"Times New Roman\",serif;color:black;'\u003eP. retention\u003csup\u003e**\u003c/sup\u003e\u003c/span\u003e\u003c/em\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 63pt;border: medium none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:150%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:center;'\u003e\u003cspan style='font-size:16px;line-height:150%;font-family:\"Times New Roman\",serif;color:black;'\u003e\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 58.5pt;border: medium none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:150%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:center;'\u003e\u003cspan style='font-size:16px;line-height:150%;font-family:\"Times New Roman\",serif;color:black;'\u003e\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 85.5pt;border: medium none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:150%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:center;'\u003e\u003cspan style='font-size:16px;line-height:150%;font-family:\"Times New Roman\",serif;color:black;'\u003e\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 76.5pt;border: medium none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:150%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:center;background:white;'\u003e\u003cspan style='font-size:16px;line-height: 150%;font-family:\"Times New Roman\",serif;color:black;'\u003e19.1\u003csup\u003eb\u003c/sup\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 76.5pt;border: medium none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:150%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:center;background:white;'\u003e\u003cspan style='font-size:16px;line-height: 150%;font-family:\"Times New Roman\",serif;color:black;'\u003e0.003\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width: 112.25pt;border: medium none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:150%;font-size:15px;font-family:\"Calibri\",sans-serif;'\u003e\u003cspan style='font-size:16px;line-height:150%;font-family:\"Times New Roman\",serif;color:black;'\u003eYes\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 63pt;border: medium none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:150%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:center;'\u003e\u003cspan style='font-size:16px;line-height: 150%;font-family:\"Times New Roman\",serif;color:black;'\u003e4\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 58.5pt;border: medium none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:150%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:center;'\u003e\u003cspan style='font-size:16px;line-height: 150%;font-family:\"Times New Roman\",serif;color:black;'\u003e2\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 85.5pt;border: medium none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:150%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:center;'\u003e\u003cspan style='font-size:16px;line-height: 150%;font-family:\"Times New Roman\",serif;color:black;'\u003e50\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 76.5pt;border: medium none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:150%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:center;'\u003e\u003cspan style='font-size:16px;line-height: 150%;font-family:\"Times New Roman\",serif;color:black;'\u003e\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 76.5pt;border: medium none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:150%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:center;'\u003e\u003cspan style='font-size:16px;line-height: 150%;font-family:\"Times New Roman\",serif;color:black;'\u003e\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width: 112.25pt;border-color: currentcolor currentcolor black;border-style: none none solid;border-width: medium medium 1pt;border-image: none 100% / 1 / 0 stretch;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:150%;font-size:15px;font-family:\"Calibri\",sans-serif;'\u003e\u003cspan style='font-size:16px;line-height:150%;font-family:\"Times New Roman\",serif;color:black;'\u003eNo\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 63pt;border-color: currentcolor currentcolor black;border-style: none none solid;border-width: medium medium 1pt;border-image: none 100% / 1 / 0 stretch;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:150%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:center;'\u003e\u003cspan style='font-size:16px;line-height:150%;font-family:\"Times New Roman\",serif;color:black;'\u003e162\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 58.5pt;border-color: currentcolor currentcolor black;border-style: none none solid;border-width: medium medium 1pt;border-image: none 100% / 1 / 0 stretch;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:150%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:center;'\u003e\u003cspan style='font-size:16px;line-height:150%;font-family:\"Times New Roman\",serif;color:black;'\u003e1\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 85.5pt;border-color: currentcolor currentcolor black;border-style: none none solid;border-width: medium medium 1pt;border-image: none 100% / 1 / 0 stretch;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:150%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:center;'\u003e\u003cspan style='font-size:16px;line-height:150%;font-family:\"Times New Roman\",serif;color:black;'\u003e0.167\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 76.5pt;border-color: currentcolor currentcolor black;border-style: none none solid;border-width: medium medium 1pt;border-image: none 100% / 1 / 0 stretch;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:150%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:center;'\u003e\u003cspan style='font-size:16px;line-height:150%;font-family:\"Times New Roman\",serif;color:black;'\u003e\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 76.5pt;border-color: currentcolor currentcolor black;border-style: none none solid;border-width: medium medium 1pt;border-image: none 100% / 1 / 0 stretch;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:150%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:center;'\u003e\u003cspan style='font-size:16px;line-height:150%;font-family:\"Times New Roman\",serif;color:black;'\u003e\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003c/tbody\u003e\n\u003c/table\u003e\n\u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:150%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cspan style='font-size:16px;line-height:150%;font-family:\"Times New Roman\",serif;'\u003eN\u003cu\u003eo\u003c/u\u003e of test: Total number of animals tested; \u003csup\u003eb\u003c/sup\u003e-Fishers exact test value, ꭓ\u003csup\u003e2\u003c/sup\u003e: Chi-square, \u003csup\u003ea-\u0026nbsp;\u003c/sup\u003eChi- square value, \u003csup\u003e*\u003c/sup\u003eSignificant; \u003csup\u003e**\u003c/sup\u003eMale animals were excluded from the total number of cattle\u003c/span\u003e\u003c/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c/p\u003e\n\u003cp id=\"isPasted\"\u003eBased on fisher exact test, different host risk factors were considered and age of the animals (2\u003csup\u003eb\u003c/sup\u003e=6.77, P\u0026le;0.02), number of parity (ꭓ\u003csup\u003e2b\u003c/sup\u003e=9.433, P\u0026le;0.004), abortion history (ꭓ\u003csup\u003e2b\u003c/sup\u003e=16.02, P\u0026le;0.002) and placental retention (ꭓ\u003csup\u003e2b\u003c/sup\u003e=19.1, P\u0026le;0.01) were found significantly associated with brucellosis seropositivity in cattle (Table 2). However, sex and body condition of the study animals did not show statistically significant association brucella seropositivity(P\u0026gt;0.05).\u0026nbsp;\u003c/p\u003e\n\u003cp\u003e\u0026nbsp;\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eFirth bias reduced logistic regression analysis of factors associated with Brucella Seropositivity\u003c/strong\u003e\u003cstrong\u003e\u0026nbsp;cattle\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c/p\u003e\n\u003cp\u003eAfter computing firth\u0026rsquo;s bias reduced logistic regression, multiparous animals (\u0026gt;5) and placental retention showed statistically significant association with brucella infection in cattle (Table 3). It was also indicated that, adult animals were 16.78 times more at risk of contracting brucellosis when compared with young one even though it doesn\u0026rsquo;t stand statistically significant. In addition, multiparous animals were 10.68 times and animals with history of placental retention were 72.72 times more due brucella infection when compared with bovine having zero parity and those with no history of placental retention respectively (Table 3).\u003c/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c/p\u003e\n\u003cp id=\"isPasted\" style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:150%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cspan style='font-size:16px;line-height:150%;font-family:\"Times New Roman\",serif;color:black;'\u003eTable 3\u003c/span\u003e\u003cspan style='font-size:16px;line-height:150%;font-family:\"Times New Roman\",serif;color:black;border:none windowtext 1.0pt;padding:0in;'\u003e: Firth bias reduced logistic regression analysis of factors associated with Brucellosis Seropositivity in cattle by CFT.\u003c/span\u003e\u003c/p\u003e\n\u003ctable id=\"isPasted\" style=\"border-collapse:collapse;border:none;\"\u003e\n \u003ctbody\u003e\n \u003ctr\u003e\n \u003ctd style=\"width: 121.5pt;border-color: black currentcolor;border-style: solid none;border-width: 1pt medium;padding: 0in 5.4pt;height: 35.5pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height: 115%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cstrong\u003e\u003cspan style='font-size:16px;line-height:115%;font-family:\"Times New Roman\",serif;color:black;'\u003eVariables\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 67.5pt;border-color: black currentcolor;border-style: solid none;border-width: 1pt medium;padding: 0in 5.4pt;height: 35.5pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height: 115%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cstrong\u003e\u003cspan style='font-size:16px;line-height:115%;font-family:\"Times New Roman\",serif;color:black;'\u003eNumber\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height: 115%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cstrong\u003e\u003cspan style='font-size:16px;line-height:115%;font-family:\"Times New Roman\",serif;color:black;'\u003etested\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 76.5pt;border-color: black currentcolor;border-style: solid none;border-width: 1pt medium;padding: 0in 5.4pt;height: 35.5pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height: 115%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cstrong\u003e\u003cspan style='font-size:16px;line-height:115%;font-family:\"Times New Roman\",serif;color:black;'\u003eSeropositive (N, %)\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 59.4pt;border-color: black currentcolor;border-style: solid none;border-width: 1pt medium;padding: 0in 5.4pt;height: 35.5pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height: 115%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cstrong\u003e\u003cspan style='font-size:16px;line-height:115%;font-family:\"Times New Roman\",serif;color:black;'\u003eOR\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 82pt;border-color: black currentcolor;border-style: solid none;border-width: 1pt medium;padding: 0in 5.4pt;height: 35.5pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height: 115%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cstrong\u003e\u003cspan style='font-size:16px;line-height:115%;font-family:\"Times New Roman\",serif;color:black;'\u003e(95%CI)\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 61.1pt;border-color: black currentcolor;border-style: solid none;border-width: 1pt medium;padding: 0in 5.4pt;height: 35.5pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height: 115%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cstrong\u003e\u003cspan style='font-size:16px;line-height:115%;font-family:\"Times New Roman\",serif;color:black;'\u003eP-value\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width: 121.5pt;border: medium none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height: 115%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cstrong\u003e\u003cspan style='font-size:16px;line-height:115%;font-family:\"Times New Roman\",serif;color:black;'\u003eAge\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 67.5pt;border: medium none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height: 115%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cspan style='font-size:16px;line-height:115%;font-family:\"Times New Roman\",serif;color:black;'\u003e\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 76.5pt;border: medium none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height: 115%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cspan style='font-size:16px;line-height:115%;font-family:\"Times New Roman\",serif;color:black;'\u003e\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 59.4pt;border: medium none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height: 115%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cspan style='font-size:16px;line-height:115%;font-family:\"Times New Roman\",serif;color:black;'\u003e\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 82pt;border: medium none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp 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style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height: 115%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cspan style='font-size:16px;line-height:115%;font-family:\"Times New Roman\",serif;color:black;'\u003eYoung\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 67.5pt;border: medium none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height: 115%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cspan style='font-size:16px;line-height:115%;font-family:\"Times New Roman\",serif;color:black;'\u003e25\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 76.5pt;border: medium none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height: 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style='font-size:16px;line-height:115%;font-family:\"Times New Roman\",serif;color:black;'\u003e1\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 61.1pt;border: medium none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height: 115%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cspan style='font-size:16px;line-height:115%;font-family:\"Times New Roman\",serif;color:black;'\u003e-\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width: 121.5pt;border: medium none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height: 115%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cspan style='font-size:16px;line-height:115%;font-family:\"Times New 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style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height: 115%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cspan style='font-size:16px;line-height:115%;font-family:\"Times New Roman\",serif;color:black;'\u003e0.0695\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width: 121.5pt;border: medium none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height: 115%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cspan style='font-size:16px;line-height:115%;font-family:\"Times New Roman\",serif;color:black;'\u003eOld\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 67.5pt;border: medium none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height: 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style='font-size:16px;line-height:115%;font-family:\"Times New Roman\",serif;color:black;'\u003e0.122\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 82pt;border: medium none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height: 115%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cspan style='font-size:16px;line-height:115%;font-family:\"Times New Roman\",serif;color:black;'\u003e(-8.586,3.93)\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 61.1pt;border: medium none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height: 115%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cspan style='font-size:16px;line-height:115%;font-family:\"Times New 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style=\"width: 82pt;border: medium none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height: 115%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cspan style='font-size:16px;line-height:115%;font-family:\"Times New Roman\",serif;color:black;'\u003e\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 61.1pt;border: medium none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height: 115%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cspan style='font-size:16px;line-height:115%;font-family:\"Times New Roman\",serif;color:black;'\u003e\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width: 121.5pt;border: medium none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height: 115%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cspan style='font-size:16px;line-height:115%;font-family:\"Times New Roman\",serif;color:black;'\u003eNull\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 67.5pt;border: medium none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height: 115%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cspan style='font-size:16px;line-height:115%;font-family:\"Times New Roman\",serif;color:black;'\u003e25\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 76.5pt;border: medium none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height: 115%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cspan style='font-size:16px;line-height:115%;font-family:\"Times New Roman\",serif;color:black;'\u003e0\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 59.4pt;border: medium none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height: 115%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cspan style='font-size:16px;line-height:115%;font-family:\"Times New Roman\",serif;color:black;'\u003e1\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 82pt;border: medium none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height: 115%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cspan style='font-size:16px;line-height:115%;font-family:\"Times New Roman\",serif;color:black;'\u003e\u0026nbsp;1\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 61.1pt;border: medium none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height: 115%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cspan style='font-size:16px;line-height:115%;font-family:\"Times New Roman\",serif;color:black;'\u003e-\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width: 121.5pt;border: medium none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height: 115%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cspan style='font-size:16px;line-height:115%;font-family:\"Times New Roman\",serif;color:black;'\u003e1-5\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 67.5pt;border: medium none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height: 115%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cspan style='font-size:16px;line-height:115%;font-family:\"Times New Roman\",serif;color:black;'\u003e116\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 76.5pt;border: medium none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height: 115%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cspan style='font-size:16px;line-height:115%;font-family:\"Times New Roman\",serif;color:black;'\u003e0\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 59.4pt;border: medium none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height: 115%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cspan style='font-size:16px;line-height:115%;font-family:\"Times New Roman\",serif;color:black;'\u003e0.007\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 82pt;border: medium none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height: 115%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cspan style='font-size:16px;line-height:115%;font-family:\"Times New Roman\",serif;color:black;'\u003e(-10.8, 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style=\"width: 67.5pt;border: medium none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height: 115%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cspan style='font-size:16px;line-height:115%;font-family:\"Times New Roman\",serif;color:black;'\u003e27\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 76.5pt;border: medium none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height: 115%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cspan style='font-size:16px;line-height:115%;font-family:\"Times New Roman\",serif;color:black;'\u003e3\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 59.4pt;border: medium none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height: 115%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cspan style='font-size:16px;line-height:115%;font-family:\"Times New Roman\",serif;color:black;'\u003e10.68\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 82pt;border: medium none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height: 115%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cspan style='font-size:16px;line-height:115%;font-family:\"Times New Roman\",serif;color:black;'\u003e(-1.19,7.595)\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 61.1pt;border: medium none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height: 115%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cspan style='font-size:16px;line-height:115%;font-family:\"Times New Roman\",serif;color:black;'\u003e0.0042\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width: 121.5pt;border: medium none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height: 115%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cstrong\u003e\u003cspan style='font-size:16px;line-height:115%;font-family:\"Times New Roman\",serif;color:black;'\u003ePlacental retention\u0026nbsp;\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 67.5pt;border: medium none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height: 115%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cspan style='font-size:16px;line-height:115%;font-family:\"Times New Roman\",serif;color:black;'\u003e\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 76.5pt;border: medium none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height: 115%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cspan style='font-size:16px;line-height:115%;font-family:\"Times New Roman\",serif;color:black;'\u003e\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 59.4pt;border: medium none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height: 115%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cspan 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Roman\",serif;color:black;'\u003e\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width: 121.5pt;border: medium none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height: 115%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cspan style='font-size:16px;line-height:115%;font-family:\"Times New Roman\",serif;color:black;'\u003eYes\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 67.5pt;border: medium none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height: 115%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cspan style='font-size:16px;line-height:115%;font-family:\"Times New Roman\",serif;color:black;'\u003e6\u003c/span\u003e\u003c/p\u003e\n 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stretch;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height: 115%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cspan style='font-size:16px;line-height:115%;font-family:\"Times New Roman\",serif;color:black;'\u003eNo\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 67.5pt;border-color: currentcolor currentcolor black;border-style: none none solid;border-width: medium medium 1pt;border-image: none 100% / 1 / 0 stretch;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height: 115%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cspan style='font-size:16px;line-height:115%;font-family:\"Times New Roman\",serif;color:black;'\u003e173\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 76.5pt;border-color: currentcolor currentcolor black;border-style: none none solid;border-width: medium medium 1pt;border-image: none 100% / 1 / 0 stretch;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height: 115%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cspan style='font-size:16px;line-height:115%;font-family:\"Times New Roman\",serif;color:black;'\u003e2\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 59.4pt;border-color: currentcolor currentcolor black;border-style: none none solid;border-width: medium medium 1pt;border-image: none 100% / 1 / 0 stretch;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height: 115%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cspan style='font-size:16px;line-height:115%;font-family:\"Times New Roman\",serif;color:black;'\u003e1\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 82pt;border-color: currentcolor currentcolor black;border-style: none none solid;border-width: medium medium 1pt;border-image: none 100% / 1 / 0 stretch;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height: 115%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cspan style='font-size:16px;line-height:115%;font-family:\"Times New Roman\",serif;color:black;'\u003e1\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 61.1pt;border-color: currentcolor currentcolor black;border-style: none none solid;border-width: medium medium 1pt;border-image: none 100% / 1 / 0 stretch;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height: 115%;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cspan style='font-size:16px;line-height:115%;font-family:\"Times New Roman\",serif;color:black;'\u003e-\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003c/tbody\u003e\n\u003c/table\u003e"},{"header":"Discussion","content":"\u003cp\u003eIn the present study, 10.5% of tested cattle were found reactive for brucella infection by RBPT among which 2.2% were confirmed to be brucella seropositive by CFT. The present study finding is in close agreement with the earlier finding of (Yimer \u003cspan citationid=\"CR38\" class=\"CitationRef\"\u003e2017\u003c/span\u003e) in Amibara, (Asmare et al. \u003cspan citationid=\"CR5\" class=\"CitationRef\"\u003e2007\u003c/span\u003e) in Sidama, (Alemu et al. \u003cspan citationid=\"CR1\" class=\"CitationRef\"\u003e2014\u003c/span\u003e) in East shoa zone, (Kassahun \u003cspan citationid=\"CR21\" class=\"CitationRef\"\u003e2004\u003c/span\u003e) in Sidama and (Jergefa et al. \u003cspan citationid=\"CR20\" class=\"CitationRef\"\u003e2009\u003c/span\u003e) in central Oromia who reported 2.4%, 2.46%, 2.0%, 2.5% and 2.9% respectively. However, it is lower when compared to the findings of (Megersa et al. \u003cspan citationid=\"CR26\" class=\"CitationRef\"\u003e2011b\u003c/span\u003e) who reported 10.6% in Borana zone, (Mekonnen et al. \u003cspan citationid=\"CR27\" class=\"CitationRef\"\u003e2010\u003c/span\u003e) who reported 4.9% in Western Tigray, (Tschopp et al. \u003cspan citationid=\"CR36\" class=\"CitationRef\"\u003e2015\u003c/span\u003e) who reported 4.8% in pastoral community of Afar and Oromia region adjacent to Awash national park and (Dinka and Chala \u003cspan citationid=\"CR13\" class=\"CitationRef\"\u003e2009\u003c/span\u003e) who reported 15.2% in East Showa zone of Oromia region .\u003c/p\u003e \u003cp\u003eThe seroprevalence result of the current study is also lower than some reports in other African countries. For instance, a prevalence of 3.4% in Cameroon by (Awah-Ndukum et al. \u003cspan citationid=\"CR6\" class=\"CitationRef\"\u003e2018\u003c/span\u003e), 24.5% in Sudan by (Angara et al. \u003cspan citationid=\"CR2\" class=\"CitationRef\"\u003e2004\u003c/span\u003e), 12\u0026ndash;15.8% in Uganda by \u003cem\u003e(\u003c/em\u003eMiller \u003cem\u003eet al.\u003c/em\u003e 2015) and 26.50 % in India by (Chand and Chhabra \u003cspan citationid=\"CR7\" class=\"CitationRef\"\u003e2013\u003c/span\u003e) were relatively higher than the present finding. The difference in brucellosis seroprevalence of the current and previous study results might be due to variation in sample size, husbandry and management practices, virulence of the organisms and diagnostic test employed. In addition to estimation of seroprevalence, this study was also carried out aiming to assess the risk factors associated with disease occurrence in cattle. Therefore; sex, age, body condition, number of parity and herd size of the cattle and history of placental retention were accessed. As a result, some of the considered risk factors were not significantly associated with brucellosis seropositivity in cattle except age, number of parities, abortion history and placental retention (Table \u003cspan refid=\"Tab2\" class=\"InternalRef\"\u003e2\u003c/span\u003e).\u003c/p\u003e \u003cp\u003eThe insignificant association of sex with brucellosis seropositivity disagree with findings of (Dinka and Chala \u003cspan citationid=\"CR13\" class=\"CitationRef\"\u003e2009\u003c/span\u003e ; Kebede et al. \u003cspan citationid=\"CR22\" class=\"CitationRef\"\u003e2008\u003c/span\u003e) who reported higher cases of brucellosis in female animals than in males. This might be due to the incomparable number of male cattle with female animals in the present study. Concerned with body condition score, 3.57% of animals with good body condition were found positive whereas no reactor animals encountered from those with poor body condition.\u003c/p\u003e \u003cp\u003eThis finding is in agreement with the report of (Awah-Ndukum et al. \u003cspan citationid=\"CR6\" class=\"CitationRef\"\u003e2018\u003c/span\u003e), who stated insignificant association of body condition score with susceptibility to brucellosis. Herd size of the cattle was also considered as risk factors since brucellosis is a disease of herd importance. Accordingly, the herd level seroprevalence was 18.18% which is in close agreement with the finding of(Ibrahim et al. \u003cspan citationid=\"CR18\" class=\"CitationRef\"\u003e2010\u003c/span\u003e). Even though large herd sizes are mor prone to brucella infection, no significant association of herd size with brucellosis seropositivity was observed in the current study(P\u0026gt;0.05). Consequently, this finding disagrees with findings of(Tolosa \u003cspan citationid=\"CR35\" class=\"CitationRef\"\u003e2004\u003c/span\u003e). After computing firth bias reduced logistic regression analysis, only multiparous and animals with history of placental retention showed statistically significant association with brucella infection(p\u0026lt;0.05).\u003c/p\u003e \u003cp\u003eThis finding is in close agreement with (Alemu et al., \u003cspan citationid=\"CR1\" class=\"CitationRef\"\u003e2014\u003c/span\u003e)and (Isloor et al., \u003cspan citationid=\"CR19\" class=\"CitationRef\"\u003e1998\u003c/span\u003e) who reported significant association of parity and placental retention with brucella infection respectively. It was also appreciated that; the owners usually keep their cattle in national park to look for water and pasture which allow close contact of domestic animals with jungle beast. Due to this, brucellosis can spread in either direction from contaminated pasture and water point or by direct contact of aborted fetal materials. Study conducted in Tanzania indicated, the interactions between wildlife and livestock as potential risk for brucellosis transmission to humans and livestock.\u003c/p\u003e \u003cp\u003eIn human, brucellosis is transmitted through consumption of unpasteurized dairy products or through direct contact with infected animals, placentas or aborted fetuses (Haileselassie et al., \u003cspan citationid=\"CR16\" class=\"CitationRef\"\u003e2011\u003c/span\u003e). Evidences shown that, the social habit of raw milk and meat consumption, unsafe handling of aborted fetuses and placenta, assisting parturition, and occupations related to animal contacts have been reported to be some important epidemiological factors for human brucellosis(Dinka and Chala \u003cspan citationid=\"CR13\" class=\"CitationRef\"\u003e2009\u003c/span\u003e; Haileselassie et al. \u003cspan citationid=\"CR16\" class=\"CitationRef\"\u003e2011\u003c/span\u003e). Study conduct by (Eshetu et al. \u003cspan citationid=\"CR15\" class=\"CitationRef\"\u003e2018\u003c/span\u003e) indicated, brucellosis were 5.11 times more in those who had assist animals during parturition compared to those who did not. This stands true for pastoral society whom believed to lead mobile lifestyle with few accesses to veterinary service. So, this condition creates a favorable condition for widespread and permanent occurrence of brucellosis in the area.\u003c/p\u003e"},{"header":"Conclusion","content":"\u003cp\u003eBrucellosis is a chronic bacterial disease of domestic and wild mammals having worldwide distribution. Cattle contract brucellosis through direct and indirect contact with infected animals and their excreta, ingestion of infected materials and sometimes aerosol transmission is expected. Based on the nature of the disease and ease of transmission, brucellosis is common in the pastoral society of Afar region due to their habit of mobile life style which allows intermingling of livestock and consumption of raw milk in human. Generally, the current study provides baseline data on cattle\u0026rsquo;s brucellosis in the current study district of Afar region, Ethiopia. Hence, working to enhance the awareness level of the pastoral society about the public health and economic significance of brucellosis through training and subsequent isolation and characterization of circulating strain and biotype is very important.\u003c/p\u003e"},{"header":"References","content":"\u003col\u003e\u003cli\u003e\u003cspan\u003eAlemu F, Admasu P, Feyera T, Niguse A (2014) \"Seroprevalence of bovine brucellosis in eastern Showa, Ethiopia\". Acad J Anim Dis 3:27\u0026ndash;32\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eAngara TEE, Ism ail AA, Agab H, Saeed NS (2004) \"Sero-prevalence of bovine brucellosis in Kuku Dairy Scheme. Khartoum North, Sudan\", pp\u0026nbsp;0 0\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eAR B, Adehossi SCE, Matthys F, Vias GF, Yenikoye a E, Thys (2014) \" La brucellose en Afrique Subsaharienne. La Brucell. en Afrique Subsaharienne\". Ann M\u0026eacute;d V\u0026eacute;t 158:139\u0026ndash;159\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eAsgedom H, Damena D, Duguma R, (2016), \"Seroprevalence of bovine brucellosis and associated risk factors in and around Alage district, Ethiopia\", \u003cem\u003eSpringerplus.\u003c/em\u003e5,851\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eAsmare K, Prasad S, Asfaw Y, Gelaye E, Ayelet G, Zeleke A (2007) \"Seroprevalence of brucellosis in cattle and in high risk animal health professionals in Sidama Zone, Southern Ethiopia. Ethiopia\"\u003cem\u003eVet J\u003c/em\u003e 11:59\u0026ndash;68\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eAwah-Ndukum J, Mouiche MMM, Kouonmo-Ngnoyum L, Bayang HN, Manchang TK, Poueme RSN, Kouamo J, Ngu-Ngwa V, Assana E, Feussom KJM (2018) Seroprevalence and risk factors of brucellosis among slaughtered indigenous cattle, abattoir personnel and pregnant women in Ngaound\u0026eacute;r\u0026eacute;, Cameroon\". BMC Infect Dis 18:611\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eChand P, Chhabra R (2013) \"Herd and individual animal prevalence of bovine brucellosis with associated risk factors on dairy farms in Haryana and Punjab in India\". Trop Anim Health Prod 45:1313\u0026ndash;1319\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eChekol W, Mnalku A (2012) \"Selected physical and chemical characteristics of soils of the middle awash irrigated farm lands, Ethiopia\". Ethiop J Agric Sci 22:127\u0026ndash;142\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eCorbel J (2006) \"Brucellosis in humans and animals\". WHO Press, Switz\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eCSA J (2007) \"Agricultural Sample Survey 2006-07\". Vol. I, Addis Ababa\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eDegefu H, Mohamud M, Hailemelekot M, Yohannes M, (2011);\"Seroprevalence of bovine brucellosis in agro pastoral areas of Jijjiga zone of Somali National Regional State, Eastern Ethiopia\", \u003cem\u003eEthiop. Vet. J\u003c/em\u003e 15\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eDeka RP, Magnusson U, Grace D, Lindahl J, Pratim R, Magnusson U, Grace D, Lindahl J,(2019), \" Bovine brucellosis: prevalence, risk factors, economic cost and control options with particular reference to India- a review\",\u003cem\u003eInfect. Ecol. Epidemiol\u003c/em\u003e 9\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eDinka H, Chala R (2009) \"Seroprevalence Study of Bovine Brucellosis in Pastoral and Agro-Pastoral Areas of East Showa Zone, Oromia Regional State, Ethiopia\", 6,508-512\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eEdao BM, Hailegebreal G, Berg S, Zewude A, Zeleke Y, Sori T, Almaw G, Whatmore AM, Ameni G, Wood JLN (2018) ),\"Brucellosis in the Addis Ababa dairy cattle: the myths and the realities\". BMC Vet Res 14:396\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eEshetu A, Belina D, Jafer M, Mengistu S (2018) Sero-Prevalence Of Brucellosis In Camels And Febrile Human. Patients Attending Health Facilities In Selected Districts Of Eastern Ethiopia\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eHaileselassie M, Kalayou S, Kyule M, Asfaha M, Belihu K, (2011), \"Effect of Brucella infection on reproduction conditions of female breeding cattle and its public health significance in Western Tigray, northern Ethiopia\",\u003cem\u003eVet. Med. Int.\u003c/em\u003e\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eIbrahim K, Thomas R, Peter K, Omer RA (2011) \"A molecular survey on cystic echinococcosis in Sinnar area, Blue Nile state (Sudan)\". Chin Med J (Engl 124:2829\u0026ndash;2833\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eIbrahim N, Belihu K, Lobago F, And MB-TAH, 2010, U., (2010), \" Sero-prevalence of bovine brucellosis and its risk factors in Jimma zone of Oromia Region, South-western Ethiopia\",\u003cem\u003eTropical Animal Health and Production\u003c/em\u003e 42,35-40\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eIsloor S, Renukaradhya GJ, Rajasekhar M (1998) A serological survey of bovine brucellosis in India. Rev Sci Tech Int des \u0026eacute;pizooties 17:781\u0026ndash;785\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eJergefa T, Kelay B, Bekana M, Teshale S, Gustafson H, Kindahl H, (2009), \" Epidemiological study of bovine brucellosis in three agro-ecological areas of central Oromiya, Ethiopia\", \u003cem\u003eRev. Sci. Tech\u003c/em\u003e 28\u0026ndash;933\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eKassahun A, (2004), \" Epidemiology of bovine brucellosis in cattle and its seroprevalence in animal health professionals in Sidama Zone, Southern Ethiopia\" \u003cem\u003eMaster Sci. thesis. Addis Ababa Univ. Fac. Vet. Med. Debre Zeit, Ethiop.\u003c/em\u003e\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eKebede T, Ejeta G, Ameni G (2008) \" Seroprevalence of bovine brucellosis in smallholder farms in central Ethiopia (Wuchale-Jida district)\". Rev Med Vet (Toulouse) 159:3\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eLakew A, Hiko A, Abraha A, Hailu SM, (2019), \" Sero-prevalence and community awareness on the risks associated with Livestock and Human brucellosis in selected districts of Fafan Zone of Ethiopian-Somali National Regional State\", \u003cem\u003eVet. Anim. Sci.\u003c/em\u003e\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eMassis F, De, Zilli K, Di G, Id D, Nuvoloni R, Pelini S, Sacchini L, Alterio ND, Giannatale E, Di, (2019), \" Distribution of Brucella field strains isolated from livestock, wildlife populations, and humans in Italy from 2007 to 2015\",\u003cem\u003ePlose one\u003c/em\u003e 1\u0026ndash;16\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eMathew C, Stokstad M, Johansen TB, Klevar S, Mdegela RH, Mwamengele G, Michel P, Escobar L, Fretin D, Godfroid J (2015) \"First isolation, identification, phenotypic and genotypic characterization of Brucella abortus biovar 3 from dairy cattle in Tanzania\". BMC Vet Res 11:1\u0026ndash;7\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eMegersa B, Biffa D, Niguse F, Rufael T, Asmare K, Skjerve E (2011b) \"Cattle brucellosis in traditional livestock husbandry practice in Southern and Eastern Ethiopia, and its zoonotic implication\". Acta Vet Scand 53:24\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eMekonnen H, Kalayou S, Kyule M (2010) \" Serological survey of bovine brucellosis in barka and arado breeds (Bos indicus) of Western Tigray, Ethiopia\". Prev Vet Med 94:28\u0026ndash;35\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eMuradrasoli S, Mugizi DR, Waiswa C, Erume J, Boqvist S, Mboowa G, Magnusson U, Nasinyama GW, Klint M,(2015), \"Isolation and Molecular Characterization of Brucella Isolates in Cattle Milk in Uganda\", \u003cem\u003eBiomed Res. Int\u003c/em\u003e 2015, 1-9\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eNielsen K (2002) \" Diagnosis of brucellosis by serology\". Vet Microbiol 90:447\u0026ndash;459\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eOIE, (2018), \" Brucellosis(B.abortus, B. melitensis, and B. suis)\"358\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eOIE A, (2008), \" Manual of diagnostic tests and vaccines for terrestrial animals\", \u003cem\u003eOff. Int. des Epizoot. paris, Fr\u003c/em\u003e 1092\u0026ndash;1106\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003ePuhr R et al (2017) \"Firth's logistic regression with rare events. accurate effect estimates\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eand predictions\",\u003cem\u003eStatistics in medicine\u003c/em\u003e 36,2302-2317\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eTibesso G, Ibrahim N, Tolosa T (2014) \" Sero-prevalence of bovine and human brucellosis in Adami Tulu, central Ethiopia\". World Appl Sci J 31:776\u0026ndash;780\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eTolosa T (2004) Seroprevalence study of bovine brucellosis and its public health significance in selected sites of Jimma Zone, Western Ethiopia. Ethiop. Msc Thesis, Addis Ababa Univ. Fac. Vet. Med. Debre Zeit\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eTschopp R, Bekele S, Moti T, Young D, Aseffa A (2015) \" Brucellosis and bovine tuberculosis prevalence in livestock from pastoralist communities adjacent to Awash National Park, Ethiopia\". Prev Vet Med 120:187\u0026ndash;194\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eTumwine G, Matovu E, Kabasa JD, Owiny DO, Majalija S (2015) \"Human brucellosis: sero-prevalence and associated risk factors in agro-pastoral communities of Kiboga District, Central Uganda\". BMC Public Health 15:900\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eYimer G, (2017), \" Seroprevalence and isolation of brucella species from camel and cattle with history af recent abortion in Amibara District, Afar Regional state\", \u003cem\u003eMsc Thesis.\u003c/em\u003e\u003c/span\u003e\u003c/li\u003e\u003c/ol\u003e"},{"header":"Declarations","content":"\u003cp\u003eThe authors declare that there is no competing interest."}],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":true,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":true,"hideJournal":true,"highlight":"","institution":"","isAcceptedByJournal":false,"isAuthorSuppliedPdf":false,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":false,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true},"keywords":"Amibara, Afar, Brucellosis, Cattle, Ethiopia, Seroprevalence","lastPublishedDoi":"10.21203/rs.3.rs-984061/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-984061/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003eBovine brucellosis is the most common but under reported bacterial diseases known to create a serious socio-economic problem in both intensive and extensive livestock production systems. A cross-sectional study was conducted to determine seroprevalence and associated risk factors of cattle brucellosis in Amibara district of Afar region, Ethiopia from October 2019 to May 2020. A total of 181 cattle sera were collected and screened using Rose Bengal Plate Test (RBPT) and reactive samples were further confirmed by complement fixation test (CFT). Risk factors associated with cattle brucellosis were assessed during serum sample collection using data collection format. As a result, the overall seroprevalence of cattle brucellosis was 10.5% and 2.2% by RBPT and CFT respectively. Assessment of potential risk factors showed that, Age (χ2=6.77, p=0.021), number of parity (χ2=9.433, p=0.004), abortion history (χ2=16, p=0.002) and history of placental retention (χ2=19.1, p=0.003) showed statistically significant association with brucellosis seropositivity in cattle. Based on firth\u0026rsquo;s bias reduced logistic regression analysis, only multiparous animal (OR=10.68, P=0.0042, 95% CI=-1.19-7.595) and animals with placental retention (OR=72.72, P=0.0026, 95% CI=1.46-9.272) showed statistically significantly association with brucella infection in cattle. In conclusion, the results of the current study indicate the presence of brucellosis in cattle in Amibara district of Afar region, Ethiopia. Hence, implementing preventive measures such as developing vaccination strategy, regular screening and culling of the reactive animal is important to create diseases free herd.\u003c/p\u003e","manuscriptTitle":"Sero epidemiology of Cattle Brucellosis and Associated Risk Factors in Amibara District of Afar Region, Ethiopia","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2021-11-12 18:14:22","doi":"10.21203/rs.3.rs-984061/v1","editorialEvents":[{"type":"communityComments","content":0}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"b957dc2d-ff2b-4b5d-b16c-5d40ae5ca10d","owner":[],"postedDate":"November 12th, 2021","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"posted","subjectAreas":[{"id":8483971,"name":"Large Animal Medicine"}],"tags":[],"updatedAt":"2021-11-12T18:14:22+00:00","versionOfRecord":[],"versionCreatedAt":"2021-11-12 18:14:22","video":"","vorDoi":"","vorDoiUrl":"","workflowStages":[]},"version":"v1","identity":"rs-984061","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-984061","identity":"rs-984061","version":["v1"]},"buildId":"GqpaHPwrfC8PjnIFayRh5","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}