Simple, Rapid, and High Efficiency Purification of M13 Phages: The Faj-elek Method

Simple, Rapid, and High Efficiency Purification of M13 Phages: The Faj-elek Method | Authorea try { document.documentElement.classList.add('js'); } catch (e) { } var _gaq = _gaq || []; _gaq.push(['_setAccount', 'G-8VDV14Y67G']); _gaq.push(['_trackPageview']); (function() { var ga = document.createElement('script'); ga.type = 'text/javascript'; ga.async = true; ga.src = ('https:' == document.location.protocol ? 'https://ssl' : 'http://www') + '.google-analytics.com/ga.js'; var s = document.getElementsByTagName('script')[0]; s.parentNode.insertBefore(ga, s); })(); Skip to main content Preprints Collections Wiley Open Research IET Open Research Ecological Society of Japan All Collections About About Authorea FAQs Contact Us Quick Search anywhere Search for preprint articles, keywords, etc. Search Search ADVANCED SEARCH SCROLL This is a preprint and has not been peer reviewed. Data may be preliminary. 15 January 2025 V1 Latest version Share on Simple, Rapid, and High Efficiency Purification of M13 Phages: The Faj-elek Method Authors : Erkan Mozioğlu 0000-0002-3027-5166 [email protected] , Gizem Kılıç , Esma Aybakan , Neda Tatlıoğlu , Gökçen Özgün , and Tanil Kocagoz Authors Info & Affiliations https://doi.org/10.22541/au.173696421.19122812/v1 324 views 118 downloads Contents Abstract Supplementary Material Information & Authors Metrics & Citations View Options References Figures Tables Media Share Abstract M13 bacteriophages are used as very important tools in molecular biology, biotechnology and, nanotechnology. Many methods have been developed so far for the purification of these phages. However, it is important that phages retain their infecting properties, especially in biotechnological applications such as phage display technology. The most widely used is the PEG precipitation method, but it has some limitations such as impurities and reduced infectivity. To overcome them, we developed a new method for purification of M13 bacteriophages using syringe filters made of cellulose acetate membranes with a pore diameter of 0.22 µm. Phages were aggregated so that they could remain on the filters and for this purpose, the pH of the phage cultures was reduced to 3. The phage cultures were filtered and then the phages were recovered in TBS buffer (pH 10.5) by reversing the filter. The recovery rate was 250% higher than the standard PEG method. This new Faj-elek method offers an alternative to existing methods, allowing cheaper, easier and faster purification of M13 phages using syringe filters available in every research laboratory. Dear Editor, I submit our study titled “ Simple, Rapid, and High Efficiency Purification of M13 Phages: The Faj-elek Method ” for your consideration for publication in your journal. M13 bacteriophages are used as very important tools used as novel therapeutic bionanomaterials. There are limitations to the existing methods for the purification of M13 phages. The most commonly used PEG precipitation method is easy but time-consuming in some cases considering the differences in application times (such as 1 hour to overnight in cold) and results in impurities due to co-purification of PEG molecules with phages. To address this, in this study we have developed a method by which M13 phages can be easily purified with syringe filters, and our results show that 250% more phages can be recovered compared to the most commonly used PEG method. We would like to announce this valuable study to scientists and researchers by your journal. As an editor, we would like to ask you to evaluate our work from this point of view: We care about the simple, and we imagine that the simpler we can make something, the more everyone can use it at low cost and with what is available in every lab. So we believe that while very complex or applications are already important, but the techniques or methods which simplify them are just as important and sometimes even more important. Best regards, Erkan Mozioğlu, PhD. Supplementary Material File (manuscript_thefajelekmethod.docx) Download 3.79 MB Information & Authors Information Version history V1 Version 1 15 January 2025 Copyright This work is licensed under a Non Exclusive No Reuse License. Keywords antimicrobial peptides aptamers bioprocess engineering biosensors biotherapeutics medical biotechnology nanobiotechnology viruses Authors Affiliations Erkan Mozioğlu 0000-0002-3027-5166 [email protected] Acibadem Universitesi View all articles by this author Gizem Kılıç View all articles by this author Esma Aybakan View all articles by this author Neda Tatlıoğlu View all articles by this author Gökçen Özgün View all articles by this author Tanil Kocagoz Acibadem University View all articles by this author Metrics & Citations Metrics Article Usage 324 views 118 downloads .FvxKWukQNSOunydq8rnd { width: 100px; } Citations Download citation Erkan Mozioğlu, Gizem Kılıç, Esma Aybakan, et al. Simple, Rapid, and High Efficiency Purification of M13 Phages: The Faj-elek Method. Authorea . 15 January 2025. DOI: https://doi.org/10.22541/au.173696421.19122812/v1 If you have the appropriate software installed, you can download article citation data to the citation manager of your choice. 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