Bacteriological and Physicochemical Parameters of Borehole Water in University of Abuja Main Campus and University of Abuja Teaching Hospital

Bacteriological and Physicochemical Parameters of Borehole Water in University of Abuja Main Campus and University of Abuja Teaching Hospital | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Bacteriological and Physicochemical Parameters of Borehole Water in University of Abuja Main Campus and University of Abuja Teaching Hospital Muhammad Aisha Lawan, Joy Chinyere Ogbu This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-7640761/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract This study assessed the bacteriological and physicochemical properties of borehole water samples collected from the University of Abuja Main Campus and the University of Abuja Teaching Hospital. Serial dilution was conducted before the samples were cultured on Nutrient Agar, MacConkey Agar and sub-cultured on Eosin Methylene Blue (EMB) Agar, and Salmonella-Shigella Agar (SSA) to reduce the bacterial load and determine the growth characteristics. Some growths were observed mainly lactose fermenting organism. Physicochemical parameters, including pH, temperature, turbidity, dissolved oxygen (DO), and heavy metals such as iron and manganese, were analyzed. Sample P exhibited higher microbial loads, with a mean colony forming unit count of 27.83, significantly higher than Sample U, which had a mean colony forming unit count of 3.17. Biochemical tests confirmed the isolates as Gram-negative, rod-shaped, bacilli, citrate-positive, oxidase negative, indole negative likely of the citrobacter genus. Oxidase negative, methyl-red negative, catalase-positive likely of the klebsiella pneumoniae genus. Antibiotic susceptibility tests revealed that all the gram negative antibiotics were effective. Statistical analysis using one-way ANOVA showed significant differences (p = 0.00025) in bacterial growth and physicochemical properties between the two sites. The pH ranged from 6.8 to 6.9, and turbidity values were higher in Sample P, indicating potential health risks. The findings underscore the importance of regular water quality monitoring, effective antibiotic use, and robust infection control strategies to mitigate public health risks associated with waterborne pathogens. The higher bacterial burden and contamination risk observed in Sample P highlight the urgent need for targeted interventions to ensure safe and sustainable water sources. Applied & Industrial Microbiology General Microbiology Applied Biochemistry Bacteriological Physicochemical University of Abuja Teaching Hospital University of Abuja Main Campus INTRODUCTION Water is a natural chemical substance which consists of the elements; hydrogen and oxygen in the ratio of two is to one (2:1). It is indispensable for man's existence on earth as about two-thirds of the human body consists of water and requires between one to seven (1–7) liters of water per day for its appropriate functioning to avoid dehydration (Mbaenyi and Egbuche, 2012). Broadly, the demand for water by man can be divided into three (3) major categories; domestic, industrial and agriculture (Jidauna et al. , 2014). A guaranteed supply of water for these purposes greatly improves the health, social and economic facets of human life (Ogunnowo, 2014). The quality of water depends on its physical, chemical, and biological characteristics, which determines its utility for different purposes (Adhikary et al. , 2020). Water fit for human consumption is referred to as potable or drinking water and should be of safe quality, which entails that it does not present any significant health risk over life time consumption (WHO, 2016). Drinking water schemes conventionally employed include surface water (rivers, streams, lakes, etc.) and groundwater (boreholes and wells). However, Agwu et al . (2013) reported that there is a rising dependence on groundwater due to increasing contamination of surface water and as it is believed to be purified as water moves down the bedrock. Ground water is an increasingly important resource all over the world. It is the subsurface water that occurs beneath the water table in soils and geological formation that are usually saturated. It supports drinking water supplies, livestock needs, irrigation, industrial and many commercial activities. Ground water is generally less susceptible to contamination and pollution when compared to surface water bodies (Zeman et al ., 2012). Ground water pollution occurs widely from diverse sources such as water disposal facilities, industrial pollution, agricultural practices, atmosphere fallout, clearing of vegetation, over abstraction of ground water and excavation below the water table (Tredoux et al. , 2017). It not only affects water quality but also threatens human health, economic development and social prosperity (Milovanovic, 2017). Microbiological health risks are associated with many aspects of water use, including drinking water in developing countries, irrigation, reuse of treated wastewater and recreational water use (Grabow, 2020). It has been reported that drinking water supplies have a long history of association with a wide spectrum of microbial infections. Some microorganisms are native or adapted to saturated sediment and rock and are present in significant numbers in most water supply aquifers and even deep geoclinal formations. Biofilm formation sometimes encourages the growth of bacteria in wells and groundwater (borehole water). Events which occur between and within bacteria and plankton populations also affect water quality (Nishiguchi, 2020). Although water is essential for life, it also remains an important source of disease transmission and a major cause of mortality in developing countries because of limitations in access and quality (Lehloesa et al. , 2000). Drinking water can become contaminated with foreign matter such as pathogenic bacteria ( Salmonella typhi, Shigella dysentariae, Escherichia coli, Klebsiella pneumonia etc.), chemical substances (fertilizer, pesticides, metals etc.) and industrial effluents or other wastes, which deteriorates its quality; rendering it unfit for its intended use (Onuorah et al. , 2018). Several new bacterial pathogens such as Legionella spp, Pseudomonas aeruginosa and Mycobacterium spp are found in drinking water usually in low numbers and grow within the distribution system biofilms.The evaluation of potable water supplies for coliform bacteria is important in determining the sanitary quality of drinking water. High coliform counts indicate a contaminated source, inadequate treatment or post treatment deficiencies. Many developing countries suffer from either chronic shortages of fresh water or the readily accessible water resources are heavily polluted. According to the WHO drinking water fact sheets, contaminated water can transmit diseases such diarrhea, cholera, dysentery, typhoid, and polio. Also, contaminated drinking-water is estimated to cause 502,000 diarrheal deaths each year (Cheesbrough, 2016). This makes it expedient to ascertain the safety of drinking water by determining the extent to which it conforms to the standards set by regulatory bodies. In Nigeria, access to safe drinking water and hygienic sanitation facilities still pose serious challenge to its rapidly growing population of more than 174 million people (Mustafa et al. , 2013). Dwellers either go to purchase water in plastic gallons or tanker delivery to homes is employed. The assessment of the health risk from naturally occurring microbes in drinking water continues to be of a high interest to microbiologists, public health practitioners and water supply regulators. High water evaporation rates increase the concentration of dissolved ions in the water, which favor high microbial growth. Ground water is invariably cleaner than surface water sources in rural areas. However, the later may need treatment to reduce the load of suspended solids and to kill microorganisms. Removal of suspended solids presents the greatest treatment challenge, and there is a need to develop and choose technologies that will be sustainable in the medium to long term. In general, complex solutions should be avoided. Most drinking waters, even with residual disinfectants, have a natural bacterial population. This is because they use naturally available carbon and nitrogen sources in water to multiply (Manaia et al. , 2020). Addition of phosphates to water bodies influences growth of bacteria even at concentrations of less than 20 jtg L−1. Animal and human wastes are major sources of nitrogen, phosphates and pathogenic bacteria contamination of aquatic environments. These nutrients can promote growth of pathogenic bacteria implicated in causing disease in humans, wildlife and domestic animals. The type of treatment technology or combination of technologies to be used depends on the quality of water to be treated. Hence, this study which is aimed at analyzing the physicochemical and bacteriological quality of borehole water from different boreholes in University of Abuja, Main Campus and University of Abuja Teaching Hospital, comparing them with set regulatory standards. MATERIALS AND METHODS Study Area The study area was the University of Abuja Teaching Hospital (UATH) and the University of Abuja Main Campus, both located in Gwagwalada, Federal Capital Territory (FCT), Abuja, Nigeria. The UATH served as a major healthcare provider, relying on borehole water for its daily operations. This study aimed to assess the bacteriological and physicochemical quality of its borehole water to ensure it met health standards for consumption and medical use. Similarly, the University of Abuja Main Campus catered to a large community of students, staff, and faculty, with borehole water being the primary source for drinking and other activities. The study evaluated the quality of borehole water on the campus to ensure its safety for the university community. Sample Collection Water samples were collected from boreholes within University of Abuja Teaching Hospital (UATH) and the University of Abuja Main Campus. Four borehole samples were obtained during the dry season to account for seasonal variations in water quality. The water samples were collected using sterilized 1-liter glass bottles to prevent contamination. Before sampling, taps and other access points were cleaned thoroughly and flushed for five minutes to ensure the water was representative of the borehole supply. The bottles were tightly sealed and labeled with the sample number, location, and date of collection. To preserve the integrity of the samples, they were transported to the laboratory in ice-packed containers. All samples were analyzed within 24 hours of collection following standard protocols (Cheesbrough, 2010; Sloat & Ziel, 2011; WHO, 2014). Physicochemical Parameter Measurement pH Measurement The pH of the water samples was measured using a portable pH meter, which determined hydrogen ion concentration through direct potentiometry. The probe was lowered directly into the water, and the readings were allowed to stabilize for approximately three minutes before recording the values to one decimal place (APHA, 2017). Temperature (°C) Water temperature was measured in the field using a portable glass thermometer. The probe was immersed directly into the water, and the readings were allowed to stabilize for about three minutes before recording the temperature to one decimal place (APHA, 2017). Turbidity (NTU) Turbidity was measured using a nephelometer, which detected the intensity of light scattered by suspended particles in the water. The results were recorded in nephelometric turbidity units (NTU) (APHA, 2017). Electrical Conductivity (S/m) Electrical conductivity was measured using a portable conductivity meter. The probe was immersed directly into the water, and readings were recorded after stabilization. Results were expressed in Siemens per meter (APHA, 2017). Total Alkalinity (mg/L) Total alkalinity was determined by titrating the water samples with a standard acid solution. A pH indicator was used to detect the endpoint, and results were recorded in milligrams per liter (mg/L) as calcium carbonate (APHA, 2017). Dissolved Oxygen (mg/L) Dissolved oxygen (DO) levels were determined using the Winkler titration method. Manganese sulfate and an alkaline potassium iodide solution were added to the water samples, followed by titration with sodium thiosulfate. Results were expressed in milligrams per liter (mg/L) (APHA, 2017). Total Hardness (mg/L) The total hardness of water samples was determined using the EDTA titration method. Results were recorded as milligrams per liter (mg/L) of calcium carbonate (APHA, 2017). Iron (mg/L) Iron concentration was measured spectrophotometrically by reacting the water samples with a phenanthroline reagent. Absorbance values were recorded, and iron levels were calculated in milligrams per liter (mg/L) (APHA, 2017). Manganese (mg/L) Manganese concentration was determined spectrophotometrically after treatment with an alkaline potassium periodate solution. Results were expressed in milligrams per liter (mg/L) (APHA, 2017). Sterilization and Preparation of Media The media used for bacteriological analysis were prepared following standard microbiological procedures to ensure reliability and accuracy in isolating and identifying bacterial contaminants. Nutrient agar, MacConkey agar, Eosin Methylene Blue (EMB) agar, and Salmonella-Shigella agar (SSA) were utilized in this study due to their specific roles in supporting bacterial growth and differentiation. Nutrient agar was prepared by dissolving the required quantity of nutrient agar powder in distilled water as per the manufacturer’s instructions. The solution was sterilized by autoclaving at 121°C for 15 minutes. Once cooled to approximately 45–50°C, the medium was poured into sterile Petri dishes and allowed to solidify. Nutrient agar served as a general-purpose medium to support the growth of a wide variety of non-fastidious bacteria and provided a baseline for bacterial enumeration and isolation. MacConkey agar was prepared similarly, using the prescribed quantity of powder dissolved in distilled water, followed by sterilization. This medium is selective and differential, designed to isolate gram-negative enteric bacteria and differentiate lactose fermenters (which produce pink colonies) from non-lactose fermenters (which form colorless colonies). Eosin Methylene Blue (EMB) agar was used to isolate and identify coliform bacteria, particularly Escherichia coli, which produces metallic green colonies due to vigorous lactose fermentation. The medium was prepared by dissolving the EMB powder in distilled water, sterilizing by autoclaving, and pouring it into sterile plates. Salmonella-Shigella agar (SSA) was employed to selectively isolate Salmonella and Shigella species. The medium contains bile salts and dyes that inhibit the growth of gram-positive bacteria, while Salmonella typically produces colorless colonies with black centers due to hydrogen sulfide production. SSA was prepared by following the manufacturer's instructions, ensuring proper sterilization and plating. All media were inspected for sterility and contamination before use. Plates were stored at room temperature and used within 48 hours to ensure the media's effectiveness. Proper preparation of these media was crucial for the accurate isolation and differentiation of bacterial contaminants in borehole water samples. Isolation of Bacteria Tenfold serial dilution of the water samples was performed. One milliliter of sterile distilled water was dispensed into a series of test tubes, and 1 mL of the water sample, serving as the stock solution, was pipetted into the first tube and mixed thoroughly. Subsequent dilutions were prepared up to 10⁻⁹. A loopful of the dilutions from 10⁻¹, 10⁻³, and 10⁻⁵ was streaked onto nutrient agar and MacConkey agar plates. The plates were incubated at 37°C for 24 hours in an inverted position. After incubation, the plates were observed for growth, and visible colonies were counted. Distinct colonies were identified based on their colonial and cultural characteristics. Further streaking was performed on eosin methylene blue (EMB) and Salmonella-Shigella agar (SSA) plates, which were incubated at 37°C for 24 hours. Colonial and cultural characteristics observed on these plates were recorded. The contamination cut-off point was taken as > 10² CFU/mL (Cheesbrough, 2010). Identification of Bacterial Isolates Bacterial isolates were identified using Gram staining, the bacteria appeared to be g-ve rod, lactose fermenting orgarnisms. Biochemical tests (e.g., catalase, oxidase, indole test, methyl-red test, voges-proskauer test and citrate test. Identification protocols adhered to Cheesbrough (2010) guidelines for bacterial species differentiation. Antibacterial Susceptibility Testing The antibacterial susceptibility test was performed using the Kirby-Bauer disk diffusion method to determine the sensitivity of bacterial isolates to commonly used antibiotics. The antibiotic disk was a gram negative disk which includ amoxicillin (30 µg), ciprofloxacin (5 µg), tetracycline (30 µg), chloramphenicol (30 µg), gentamicin (10 µg), and erythromycin (15 µg). These antibiotics were selected based on their relevance in clinical treatments and guidelines for bacterial infections (Cheesbrough, 2010). A standardized suspension of each bacterial isolate was prepared by adjusting its turbidity to match 0.5 McFarland standard. Mueller-Hinton agar plates were prepared according to the manufacturer’s instructions and were allowed to solidify. Using a sterile swab, the bacterial suspension was evenly spread across the agar surface to create a uniform lawn. Antibiotic disks were then aseptically placed on the agar surface using sterile forceps, ensuring even spacing between disks. The plates were incubated at 37°C for 24 hours. After incubation, the diameter of the zone of inhibition around each antibiotic disk was measured in millimeters using a ruler. Results were interpreted according to the Clinical and Laboratory Standards Institute (CLSI) guidelines (CLSI, 2019), which classify bacterial isolates as susceptible, intermediate, or resistant to each antibiotic. RESULTS Physicochemical Parameters The physicochemical parameters were measured to evaluate water quality. These included pH, temperature, turbidity, electrical conductivity, dissolved oxygen, and levels of heavy metals like iron and manganese. The pH was similar at both sites (6.8 at UATH and 6.9 at Psite), indicating neutral water. Temperature was slightly higher at UATH (30.0°C) than Psite (29.0°C). Turbidity was higher at UATH (2.41 NTU) than Psite (0.88 NTU), suggesting more suspended particles at UATH. Electrical conductivity and total alkalinity were almost identical at both sites, showing similar ion concentrations and buffering capacities. Dissolved oxygen levels were nearly the same (UATH: 6.53 mg/L, Psite: 6.52 mg/L), indicating healthy water quality. Total hardness was lower at UATH (52.0 mg/L) compared to Psite (62.0 mg/L). Iron and manganese levels were slightly higher at Psite but within acceptable limits. Physicochemical Parameter of boreholes water samples Parameter UATH Psite pH 6.8 6.9 Temperature (°C) 30.0 29.0 Turbidity (NTU) 2.41 0.88 Electrical Conductivity (s/m) 9.01 8.90 Total Alkalinity (mg/L) 38.0 44.0 Dissolved Oxygen (mg/L) 6.53 6.52 Total Hardness (mg/L) 52.0 62.0 Iron (mg/L) 0.25 0.28 Manganese (mg/L) 0.32 0.36 Total Viable Counts The growth and colony characteristics of Sample U and Sample P were assessed using various media to evaluate their microbiological profiles. The analysis was performed on nutrient agar, MacConkey agar, Eosin Methelene Blue (EMB) agar, Salmonella Shigella agar (SSA) and the results showed distinct differences in growth between the two samples. Growth on Nutrient Agar The table below summarizes the growth characteristics of Sample U and Sample P on nutrient agar. Sample U showed minimal growth, with off-white colonies observed at dilution 10¹ (8 colonies), and small growth at dilution 10³ (3 colonies), while no growth was recorded at dilution 10 5 . In contrast, Sample P exhibited more abundant growth, with much larger colonies observed at all dilutions: 39 colonies at dilution 10¹, 22 colonies at dilution 10³, and 17 colonies at dilution 10 5 . Cultural Characteristics/ Morphology of Bacterial Isolates on Nutrient Agar Growth on MacConkey Agar MacConkey agar is used to differentiate bacteria based on their ability to ferment lactose, and the results of the growth on these medium revealed distinct variations in lactose fermentation between the samples. Sample U exhibited moderate lactose fermentation at the highest dilution (10¹), with a pinkish mucoid appearance, which became less pronounced at lower dilutions. At dilution 10³, lactose fermentation was minimal, and by dilution 10 5 , only slight fermentation was observed, indicating a lower capacity for lactose fermentation. In contrast, Sample P displayed much stronger lactose fermentation at all dilutions. At dilution 10¹, it showed a very pinkish color, indicating strong fermentation. The pinkish color remained prominent at dilution 10³, though less intense, and at 10 5 , there was still noticeable fermentation, though reduced. These results suggest that Sample P has a more robust ability to ferment lactose compared to Sample U, which displayed weaker fermentation capabilities as dilution increased. This difference in lactose fermentation between the two samples could suggest variations in bacterial composition or metabolic activity. Cultural Characteristics/ Morphology of Bacterial Isolates on MacConkey Agar Cultural Characteristics/ Morphology of Bacterial Isolates on Eosin Methylene Blue (EMB) Agar and Salmonella Shigella Agar (SSA) Subculturing on Eosin Methylene Blue (EMB) Agar and Salmonella Shigella Agar (SSA) media provided additional insights into the microbial characteristics of Samples U and P. The results confirmed the presence of specific bacterial traits in both samples. On EMB agar, Sample U exhibited consistent purple mucoid colonies across all dilutions (10¹, 10³, and 10 5 ), which is indicative of Klebsiella species and citrobacter species, known for their ability to ferment lactose and produce mucoid colonies. Similarly, Sample P also showed purple mucoid colonies at all dilutions, further suggesting the presence of lactose-fermenting bacteria, though the specific species was not identified. Both samples showed no growth on SSA agar at any dilution (10¹, 10³, and 10 5 ), which is indicative of the absence of Salmonella or Shigella species, as these pathogens typically grow on SSA. The lack of growth on SSA could also imply that these samples do not harbor these pathogens under the conditions tested. These subculturing results reinforce the initial findings and provide more clarity on the microbial composition of the samples, highlighting the presence of Klebsiella and citrobacter in both samples, but no indication of Salmonella or Shigella contamination. Cultural Characteristics/Morphology of Bacterial Isolates on Eosin Methylene Blue (EMB) Agar Cultural Characteristics/ Morphology of Bacterial Isolates on Salmonella Shigella (SSA) Biochemical Tests Biochemical tests were conducted to identify and characterize bacterial isolates from the samples. These tests evaluated oxidase activity, catalase production, and Gram staining results to help classify the microbes. For the oxidase test , both UATH and Psite samples (from EMB and MacConkey media) showed negative results, indicating that the bacteria do not produce oxidase. In the catalase test , UATH isolates on EMB and Psite isolates on EMB were positive, forming bubbles, suggesting the presence of catalase. However, Psite isolates on MacConkey and UATH isolates on MacConkey were negative, showing no bubble formation. Lastly, Gram staining revealed that both UATH and Psite isolates were Gram-negative, rod-shaped bacteria. These tests help confirm the bacterial characteristics of the isolates, providing insights into their classification. Biochemical Identification of Bacterial Isolate Key : Isolates, GR(Gram Reaction), Oxidase test, Catalase test, I(Indole test), VP(Voges-Proskauer), Citrate test, MR (Methyl-red test), PO (Probable Organism). + = positive - = negative Antibiotic Susceptibility Test The antibiotic susceptibility test was conducted using negative (-ve) discs to assess the resistance and susceptibility profiles of the bacterial isolates from UATH and Psite. The results are summarized in the table below Both UATH and Psite displayed uniform susceptibility across all antibiotics tested, including PEF(pefloxacin), CN(gentamicin), OFX(tarivid), SXT(septrin), AM(amoxacilin), CH(chloramphenicol), CPX(ciprofloxacin), S(streptomycin), SP(sparfloxacin), and AU(augmentin). This suggests that the isolates from both locations were susceptible to some of the antibiotics, indicating a broader effectiveness of these drugs against the bacterial strains present at both sites. These results highlight the consistent susceptibility and some resistance on negative discs using the CLSI guideline, however, indicates that there are antibiotics that may still be effective for treatment. Antibiotic Susceptibility profile of Gram Negative Bacterial Isolate Key: Susceptible (S): The antibiotic is effective at inhibiting bacterial growth under standard testing conditions. Intermediate (I): The organism shows partial susceptibility. This might indicate that the antibiotic could work at higher doses or under specific conditions. Resistant (R): The antibiotic is not effective in inhibiting the growth of the organism. Antibiotic Susceptibility Profile of Gram Negative Bacterial Isolates Key : Susceptible (S): The antibiotic is effective against the organism. Intermediate (I): The organism has partial susceptibility, and higher doses of the antibiotic or different conditions might be required for efficacy. Resistant (R): The antibiotic is ineffective at inhibiting bacterial growth for this organism. Discussion This study assessed the microbiological profiles and antibiotic susceptibility of bacterial isolates from borehole water samples collected from the University of Abuja Main Campus (Sample P) and the Teaching Hospital (Sample U). Sample P showed significantly higher bacterial loads across all media compared to Sample U, suggesting higher contamination levels. Variations in colony morphology and lactose fermentation further indicated differences in bacterial composition. Biochemical tests identified catalase-positive, Gram-negative, rod-shaped bacteria likely of the Klebsiella pnuemoniae and citrobacter genus, which are common in contaminated water and linked to various infections. This finding aligns with previous studies highlighting the health risks posed by Klebsiella pnuemoniae and citrobacter genus contamination in water sources (Bassey et al ., 2017). The detection of antibiotic-resistant bacteria in borehole water is concerning and reflects global trends in antimicrobial resistance, as reported by Founou et al . (2018). Although pH levels for both samples were within the acceptable range (6.8–6.9), the elevated bacterial load in Sample P highlights the need for improved water management practices, including routine monitoring, sanitation, and disinfection. Similar recommendations have been made in previous studies on waterborne pathogens in Nigeria (Onuoha et al ., 2021; WHO, 2017). This study emphasizes the importance of monitoring borehole water quality and implementing effective treatment strategies to reduce public health risks, especially regarding bacterial contamination and antibiotic resistance (Ojo et al ., 2019). Conclusion The study highlights critical differences in bacterial load and antibiotic susceptibility between the analyzed samples. Sample P demonstrated a higher microbial burden compared to Sample U, indicating a potentially greater risk of contamination in environments represented by Sample P. The pinkish mucoid colonies on MacConkey and purple mucoid colonies on EMB agar suggest the presence of lactose-fermenting bacteria such as Klebsiella spp. These bacteria are known to be opportunistic pathogens. The study highlights the need for judicious antibiotic use and the adoption of robust infection control measures. Recommendations Regular microbiological screening of environmental and clinical samples should be carried out to detect emerging bacterial contaminants and antibiotic resistance trends. Strict hygiene protocols should be enforced in environments associated with Sample P to reduce bacterial loads and prevent contamination. Initiatives to regulate the use of antibiotics should be strengthened to prevent the escalation of resistant strains. 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Zeman, C., Kross, B., & Vlad, M. (2012). A nested case-control study of methemoglobinemia factors in children in Transylvania, Romania. Environmental Health Perspectives, 110 (8), 81-822. Additional Declarations The authors declare no competing interests. Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-7640761","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":516572058,"identity":"d7746d23-ba12-4295-bf22-0feb6f24fb8c","order_by":0,"name":"Muhammad Aisha Lawan","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAAA/0lEQVRIiWNgGAWjYDACCcYGKIuHgSGhAkgzMzfgVo6h5cEZkBa4CC4tcBYPA+PDNhCDgBb+2c1tEh/32DDw8689JpE4rzaavx2o5UfFNtyW3DnYJjnjWRqD5Ix3aRKJ247nzjjM2MDYc+Y2Ti0GEolt0jwHDjMY3DhjBtRyLLcBqIWZsY2Alj8H/kO1zDmWO58oLQwHDjAYnO8Bammoyd1ASIvEjcRmy54DyTySM/iSLRKOHcjdCNRyEJ9f+GekP7zx44CdHD//2YM3f9TU5c47f/jggx8VuLUAAQsoangYJBJAnMNgoQP41AMB8weIfWB1dQQUj4JRMApGwUgEANHRXnbkEC8JAAAAAElFTkSuQmCC","orcid":"","institution":"University of Abuja","correspondingAuthor":true,"prefix":"","firstName":"Muhammad","middleName":"Aisha","lastName":"Lawan","suffix":""},{"id":516572059,"identity":"0061821f-45f0-4a6a-be73-8063a4eea5eb","order_by":1,"name":"Joy Chinyere Ogbu","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAABAUlEQVRIiWNgGAWjYDACCcYGKIuHgfnnHxsgg7HxAPFaGBvSQFoaCGiBs8BaDoOZeLXwz25ue8xTcU+On3/tMenCHeft1rYfBtpSYxON05I7B9uNec4UG0vOeJcmPfPM7eRtZxKBWo6l5Tbg0GIgkdgmzduWkLjhxhkzCR6228lmB4BagC4koOVfQj1Uy7lks/MPidHSkJBgcL7HDGjdATuzGwRskbiR2CY551iC4cwZfMmWM84kJ5jdANqSgMcv/DPSn0m8qUmQ5+c/e/DGhwo7e7Pz6Q8ffKixwakFBJh4wPYlgDmJYJUJeJSDAOMPsH0HwBx7AopHwSgYBaNgBAIAHB1lakcFiPYAAAAASUVORK5CYII=","orcid":"","institution":"University of Abuja","correspondingAuthor":true,"prefix":"","firstName":"Joy","middleName":"Chinyere","lastName":"Ogbu","suffix":""}],"badges":[],"createdAt":"2025-09-17 13:32:46","currentVersionCode":1,"declarations":{"humanSubjects":false,"vertebrateSubjects":true,"conflictsOfInterestStatement":false,"humanSubjectEthicalGuidelines":false,"humanSubjectConsent":false,"humanSubjectClinicalTrial":false,"humanSubjectCaseReport":false,"vertebrateSubjectEthicalGuidelines":true},"doi":"10.21203/rs.3.rs-7640761/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-7640761/v1","draftVersion":[],"editorialEvents":[],"editorialNote":"","failedWorkflow":false,"files":[{"id":91596691,"identity":"c7871211-d6ea-4a4b-a555-95a96a693787","added_by":"auto","created_at":"2025-09-18 07:50:31","extension":"pdf","order_by":0,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":843673,"visible":true,"origin":"","legend":"","description":"","filename":"manuscript.pdf","url":"https://assets-eu.researchsquare.com/files/rs-7640761/v1/fcbf8b85-6ea7-44d1-bf2a-891fb8c31b2a.pdf"}],"financialInterests":"The authors declare no competing interests.","formattedTitle":"\u003cp\u003eBacteriological and Physicochemical Parameters of Borehole Water in University of Abuja Main Campus and University of Abuja Teaching Hospital","fulltext":[{"header":"INTRODUCTION","content":"\u003cp\u003eWater is a natural chemical substance which consists of the elements; hydrogen and oxygen in the ratio of two is to one (2:1). It is indispensable for man's existence on earth as about two-thirds of the human body consists of water and requires between one to seven (1–7) liters of water per day for its appropriate functioning to avoid dehydration (Mbaenyi and Egbuche, 2012). Broadly, the demand for water by man can be divided into three (3) major categories; domestic, industrial and agriculture (Jidauna \u003cem\u003eet al.\u003c/em\u003e, 2014). A guaranteed supply of water for these purposes greatly improves the health, social and economic facets of human life (Ogunnowo, 2014). The quality of water depends on its physical, chemical, and biological characteristics, which determines its utility for different purposes (Adhikary \u003cem\u003eet al.\u003c/em\u003e, 2020). Water fit for human consumption is referred to as potable or drinking water and should be of safe quality, which entails that it does not present any significant health risk over life time consumption (WHO, 2016). Drinking water schemes conventionally employed include surface water (rivers, streams, lakes, etc.) and groundwater (boreholes and wells). However, Agwu \u003cem\u003eet al\u003c/em\u003e. (2013) reported that there is a rising dependence on groundwater due to increasing contamination of surface water and as it is believed to be purified as water moves down the bedrock. Ground water is an increasingly important resource all over the world. It is the subsurface water that occurs beneath the water table in soils and geological formation that are usually saturated. It supports drinking water supplies, livestock needs, irrigation, industrial and many commercial activities. Ground water is generally less susceptible to contamination and pollution when compared to surface water bodies (Zeman \u003cem\u003eet al\u003c/em\u003e., 2012). Ground water pollution occurs widely from diverse sources such as water disposal facilities, industrial pollution, agricultural practices, atmosphere fallout, clearing of vegetation, over abstraction of ground water and excavation below the water table (Tredoux \u003cem\u003eet al.\u003c/em\u003e, 2017). It not only affects water quality but also threatens human health, economic development and social prosperity (Milovanovic, 2017). Microbiological health risks are associated with many aspects of water use, including drinking water in developing countries, irrigation, reuse of treated wastewater and recreational water use (Grabow, 2020). It has been reported that drinking water supplies have a long history of association with a wide spectrum of microbial infections. Some microorganisms are native or adapted to saturated sediment and rock and are present in significant numbers in most water supply aquifers and even deep geoclinal formations. Biofilm formation sometimes encourages the growth of bacteria in wells and groundwater (borehole water). Events which occur between and within bacteria and plankton populations also affect water quality (Nishiguchi, 2020). Although water is essential for life, it also remains an important source of disease transmission and a major cause of mortality in developing countries because of limitations in access and quality (Lehloesa \u003cem\u003eet al.\u003c/em\u003e, 2000). Drinking water can become contaminated with foreign matter such as pathogenic bacteria (\u003cem\u003eSalmonella typhi, Shigella dysentariae, Escherichia coli, Klebsiella pneumonia\u003c/em\u003e etc.), chemical substances (fertilizer, pesticides, metals etc.) and industrial effluents or other wastes, which deteriorates its quality; rendering it unfit for its intended use (Onuorah \u003cem\u003eet al.\u003c/em\u003e, 2018). Several new bacterial pathogens such as \u003cem\u003eLegionella spp, Pseudomonas aeruginosa and Mycobacterium spp\u003c/em\u003e are found in drinking water usually in low numbers and grow within the distribution system biofilms.The evaluation of potable water supplies for coliform bacteria is important in determining the sanitary quality of drinking water. High coliform counts indicate a contaminated source, inadequate treatment or post treatment deficiencies. Many developing countries suffer from either chronic shortages of fresh water or the readily accessible water resources are heavily polluted. According to the WHO drinking water fact sheets, contaminated water can transmit diseases such diarrhea, cholera, dysentery, typhoid, and polio. Also, contaminated drinking-water is estimated to cause 502,000 diarrheal deaths each year (Cheesbrough, 2016). This makes it expedient to ascertain the safety of drinking water by determining the extent to which it conforms to the standards set by regulatory bodies. In Nigeria, access to safe drinking water and hygienic sanitation facilities still pose serious challenge to its rapidly growing population of more than 174\u0026nbsp;million people (Mustafa \u003cem\u003eet al.\u003c/em\u003e, 2013). Dwellers either go to purchase water in plastic gallons or tanker delivery to homes is employed. The assessment of the health risk from naturally occurring microbes in drinking water continues to be of a high interest to microbiologists, public health practitioners and water supply regulators. High water evaporation rates increase the concentration of dissolved ions in the water, which favor high microbial growth. Ground water is invariably cleaner than surface water sources in rural areas. However, the later may need treatment to reduce the load of suspended solids and to kill microorganisms. Removal of suspended solids presents the greatest treatment challenge, and there is a need to develop and choose technologies that will be sustainable in the medium to long term. In general, complex solutions should be avoided. Most drinking waters, even with residual disinfectants, have a natural bacterial population. This is because they use naturally available carbon and nitrogen sources in water to multiply (Manaia \u003cem\u003eet al.\u003c/em\u003e, 2020). Addition of phosphates to water bodies influences growth of bacteria even at concentrations of less than 20 jtg L−1. Animal and human wastes are major sources of nitrogen, phosphates and pathogenic bacteria contamination of aquatic environments. These nutrients can promote growth of pathogenic bacteria implicated in causing disease in humans, wildlife and domestic animals. The type of treatment technology or combination of technologies to be used depends on the quality of water to be treated. Hence, this study which is aimed at analyzing the physicochemical and bacteriological quality of borehole water from different boreholes in University of Abuja, Main Campus and University of Abuja Teaching Hospital, comparing them with set regulatory standards.\u003c/p\u003e"},{"header":"MATERIALS AND METHODS","content":"\u003cp\u003eStudy Area\u003c/p\u003e\u003cp\u003eThe study area was the University of Abuja Teaching Hospital (UATH) and the University of Abuja Main Campus, both located in Gwagwalada, Federal Capital Territory (FCT), Abuja, Nigeria. The UATH served as a major healthcare provider, relying on borehole water for its daily operations. This study aimed to assess the bacteriological and physicochemical quality of its borehole water to ensure it met health standards for consumption and medical use. Similarly, the University of Abuja Main Campus catered to a large community of students, staff, and faculty, with borehole water being the primary source for drinking and other activities. The study evaluated the quality of borehole water on the campus to ensure its safety for the university community.\u003c/p\u003e\u003cp\u003eSample Collection\u003c/p\u003e\u003cp\u003eWater samples were collected from boreholes within University of Abuja Teaching Hospital (UATH) and the University of Abuja Main Campus. Four borehole samples were obtained during the dry season to account for seasonal variations in water quality. The water samples were collected using sterilized 1-liter glass bottles to prevent contamination. Before sampling, taps and other access points were cleaned thoroughly and flushed for five minutes to ensure the water was representative of the borehole supply. The bottles were tightly sealed and labeled with the sample number, location, and date of collection. To preserve the integrity of the samples, they were transported to the laboratory in ice-packed containers. All samples were analyzed within 24 hours of collection following standard protocols (Cheesbrough, 2010; Sloat \u0026amp; Ziel, 2011; WHO, 2014).\u003c/p\u003e\u003cp\u003ePhysicochemical Parameter Measurement\u003c/p\u003e\u003cp\u003epH Measurement\u003c/p\u003e\u003cp\u003eThe pH of the water samples was measured using a portable pH meter, which determined hydrogen ion concentration through direct potentiometry. The probe was lowered directly into the water, and the readings were allowed to stabilize for approximately three minutes before recording the values to one decimal place (APHA, 2017).\u003c/p\u003e\u003cp\u003eTemperature (°C)\u003c/p\u003e\u003cp\u003eWater temperature was measured in the field using a portable glass thermometer. The probe was immersed directly into the water, and the readings were allowed to stabilize for about three minutes before recording the temperature to one decimal place (APHA, 2017).\u003c/p\u003e\u003cp\u003eTurbidity (NTU)\u003c/p\u003e\u003cp\u003eTurbidity was measured using a nephelometer, which detected the intensity of light scattered by suspended particles in the water. The results were recorded in nephelometric turbidity units (NTU) (APHA, 2017).\u003c/p\u003e\u003cp\u003eElectrical Conductivity (S/m)\u003c/p\u003e\u003cp\u003eElectrical conductivity was measured using a portable conductivity meter. The probe was immersed directly into the water, and readings were recorded after stabilization. Results were expressed in Siemens per meter (APHA, 2017).\u003c/p\u003e\u003cp\u003eTotal Alkalinity (mg/L)\u003c/p\u003e\u003cp\u003eTotal alkalinity was determined by titrating the water samples with a standard acid solution. A pH indicator was used to detect the endpoint, and results were recorded in milligrams per liter (mg/L) as calcium carbonate (APHA, 2017).\u003c/p\u003e\u003cp\u003eDissolved Oxygen (mg/L)\u003c/p\u003e\u003cp\u003eDissolved oxygen (DO) levels were determined using the Winkler titration method. Manganese sulfate and an alkaline potassium iodide solution were added to the water samples, followed by titration with sodium thiosulfate. Results were expressed in milligrams per liter (mg/L) (APHA, 2017).\u003c/p\u003e\u003cp\u003eTotal Hardness (mg/L)\u003c/p\u003e\u003cp\u003eThe total hardness of water samples was determined using the EDTA titration method. Results were recorded as milligrams per liter (mg/L) of calcium carbonate (APHA, 2017).\u003c/p\u003e\u003cp\u003eIron (mg/L)\u003c/p\u003e\u003cp\u003eIron concentration was measured spectrophotometrically by reacting the water samples with a phenanthroline reagent. Absorbance values were recorded, and iron levels were calculated in milligrams per liter (mg/L) (APHA, 2017).\u003c/p\u003e\u003cp\u003eManganese (mg/L)\u003c/p\u003e\u003cp\u003eManganese concentration was determined spectrophotometrically after treatment with an alkaline potassium periodate solution. Results were expressed in milligrams per liter (mg/L) (APHA, 2017).\u003c/p\u003e\u003cp\u003eSterilization and Preparation of Media\u003c/p\u003e\u003cp\u003eThe media used for bacteriological analysis were prepared following standard microbiological procedures to ensure reliability and accuracy in isolating and identifying bacterial contaminants. Nutrient agar, MacConkey agar, Eosin Methylene Blue (EMB) agar, and Salmonella-Shigella agar (SSA) were utilized in this study due to their specific roles in supporting bacterial growth and differentiation.\u003c/p\u003e\u003cp\u003eNutrient agar was prepared by dissolving the required quantity of nutrient agar powder in distilled water as per the manufacturer’s instructions. The solution was sterilized by autoclaving at 121°C for 15 minutes. Once cooled to approximately 45–50°C, the medium was poured into sterile Petri dishes and allowed to solidify. Nutrient agar served as a general-purpose medium to support the growth of a wide variety of non-fastidious bacteria and provided a baseline for bacterial enumeration and isolation.\u003c/p\u003e\u003cp\u003eMacConkey agar was prepared similarly, using the prescribed quantity of powder dissolved in distilled water, followed by sterilization. This medium is selective and differential, designed to isolate gram-negative enteric bacteria and differentiate lactose fermenters (which produce pink colonies) from non-lactose fermenters (which form colorless colonies).\u003c/p\u003e\u003cp\u003eEosin Methylene Blue (EMB) agar was used to isolate and identify coliform bacteria, particularly Escherichia coli, which produces metallic green colonies due to vigorous lactose fermentation. The medium was prepared by dissolving the EMB powder in distilled water, sterilizing by autoclaving, and pouring it into sterile plates.\u003c/p\u003e\u003cp\u003eSalmonella-Shigella agar (SSA) was employed to selectively isolate Salmonella and Shigella species. The medium contains bile salts and dyes that inhibit the growth of gram-positive bacteria, while Salmonella typically produces colorless colonies with black centers due to hydrogen sulfide production. SSA was prepared by following the manufacturer's instructions, ensuring proper sterilization and plating.\u003c/p\u003e\u003cp\u003eAll media were inspected for sterility and contamination before use. Plates were stored at room temperature and used within 48 hours to ensure the media's effectiveness. Proper preparation of these media was crucial for the accurate isolation and differentiation of bacterial contaminants in borehole water samples.\u003c/p\u003e\u003cp\u003eIsolation of Bacteria\u003c/p\u003e\u003cp\u003eTenfold serial dilution of the water samples was performed. One milliliter of sterile distilled water was dispensed into a series of test tubes, and 1 mL of the water sample, serving as the stock solution, was pipetted into the first tube and mixed thoroughly. Subsequent dilutions were prepared up to 10⁻⁹. A loopful of the dilutions from 10⁻¹, 10⁻³, and 10⁻⁵ was streaked onto nutrient agar and MacConkey agar plates. The plates were incubated at 37°C for 24 hours in an inverted position. After incubation, the plates were observed for growth, and visible colonies were counted. Distinct colonies were identified based on their colonial and cultural characteristics. Further streaking was performed on eosin methylene blue (EMB) and Salmonella-Shigella agar (SSA) plates, which were incubated at 37°C for 24 hours. Colonial and cultural characteristics observed on these plates were recorded. The contamination cut-off point was taken as \u0026gt; 10² CFU/mL (Cheesbrough, 2010).\u003c/p\u003e\u003cp\u003eIdentification of Bacterial Isolates\u003c/p\u003e\u003cp\u003eBacterial isolates were identified using Gram staining, the bacteria appeared to be g-ve rod, lactose fermenting orgarnisms. Biochemical tests (e.g., catalase, oxidase, indole test, methyl-red test, voges-proskauer test and citrate test. Identification protocols adhered to Cheesbrough (2010) guidelines for bacterial species differentiation.\u003c/p\u003e\u003cp\u003eAntibacterial Susceptibility Testing\u003c/p\u003e\u003cp\u003eThe antibacterial susceptibility test was performed using the Kirby-Bauer disk diffusion method to determine the sensitivity of bacterial isolates to commonly used antibiotics. The antibiotic disk was a gram negative disk which includ amoxicillin (30 µg), ciprofloxacin (5 µg), tetracycline (30 µg), chloramphenicol (30 µg), gentamicin (10 µg), and erythromycin (15 µg). These antibiotics were selected based on their relevance in clinical treatments and guidelines for bacterial infections (Cheesbrough, 2010).\u003c/p\u003e\u003cp\u003eA standardized suspension of each bacterial isolate was prepared by adjusting its turbidity to match 0.5 McFarland standard. Mueller-Hinton agar plates were prepared according to the manufacturer’s instructions and were allowed to solidify. Using a sterile swab, the bacterial suspension was evenly spread across the agar surface to create a uniform lawn. Antibiotic disks were then aseptically placed on the agar surface using sterile forceps, ensuring even spacing between disks. The plates were incubated at 37°C for 24 hours. After incubation, the diameter of the zone of inhibition around each antibiotic disk was measured in millimeters using a ruler. Results were interpreted according to the Clinical and Laboratory Standards Institute (CLSI) guidelines (CLSI, 2019), which classify bacterial isolates as susceptible, intermediate, or resistant to each antibiotic.\u003c/p\u003e"},{"header":"RESULTS","content":"\u003cp\u003e\u003cstrong\u003ePhysicochemical Parameters\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eThe physicochemical parameters were measured to evaluate water quality. These included pH, temperature, turbidity, electrical conductivity, dissolved oxygen, and levels of heavy metals like iron and manganese.\u003c/p\u003e\n\u003cp\u003eThe pH was similar at both sites (6.8 at UATH and 6.9 at Psite), indicating neutral water. Temperature was slightly higher at UATH (30.0\u0026deg;C) than Psite (29.0\u0026deg;C). Turbidity was higher at UATH (2.41 NTU) than Psite (0.88 NTU), suggesting more suspended particles at UATH.\u003c/p\u003e\n\u003cp\u003eElectrical conductivity and total alkalinity were almost identical at both sites, showing similar ion concentrations and buffering capacities. Dissolved oxygen levels were nearly the same (UATH: 6.53 mg/L, Psite: 6.52 mg/L), indicating healthy water quality. Total hardness was lower at UATH (52.0 mg/L) compared to Psite (62.0 mg/L). Iron and manganese levels were slightly higher at Psite but within acceptable limits.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003ePhysicochemical Parameter of boreholes water samples\u003c/strong\u003e\u003c/p\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\" width=\"575\"\u003e\n \u003ctbody\u003e\n \u003ctr\u003e\n \u003ctd\u003e\n \u003cp\u003e\u003cstrong\u003eParameter\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e\u003cstrong\u003eUATH\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e\u003cstrong\u003ePsite\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003e\n \u003cp\u003e\u003cstrong\u003epH\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e6.8\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e6.9\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003e\n \u003cp\u003e\u003cstrong\u003eTemperature (\u0026deg;C)\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e30.0\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e29.0\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003e\n \u003cp\u003e\u003cstrong\u003eTurbidity (NTU)\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e2.41\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e0.88\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003e\n \u003cp\u003e\u003cstrong\u003eElectrical Conductivity (s/m)\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e9.01\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e8.90\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003e\n \u003cp\u003e\u003cstrong\u003eTotal Alkalinity (mg/L)\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e38.0\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e44.0\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003e\n \u003cp\u003e\u003cstrong\u003eDissolved Oxygen (mg/L)\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e6.53\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e6.52\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003e\n \u003cp\u003e\u003cstrong\u003eTotal Hardness (mg/L)\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e52.0\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e62.0\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003e\n \u003cp\u003e\u003cstrong\u003eIron (mg/L)\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e0.25\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e0.28\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003e\n \u003cp\u003e\u003cstrong\u003eManganese (mg/L)\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e0.32\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e0.36\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003c/tbody\u003e\n\u003c/table\u003e\n\u003cp\u003e\u003cstrong\u003eTotal Viable Counts\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eThe growth and colony characteristics of Sample U and Sample P were assessed using various media to evaluate their microbiological profiles. The analysis was performed on nutrient agar, MacConkey agar, Eosin Methelene Blue (EMB) agar, \u003cem\u003eSalmonella Shigella\u003c/em\u003e agar (SSA) and the results showed distinct differences in growth between the two samples.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eGrowth on Nutrient Agar\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eThe table below summarizes the growth characteristics of Sample U and Sample P on nutrient agar. Sample U showed minimal growth, with off-white colonies observed at dilution 10\u0026sup1; (8 colonies), and small growth at dilution 10\u0026sup3; (3 colonies), while no growth was recorded at dilution 10\u003csup\u003e5\u003c/sup\u003e. In contrast, Sample P exhibited more abundant growth, with much larger colonies observed at all dilutions: 39 colonies at dilution 10\u0026sup1;, 22 colonies at dilution 10\u0026sup3;, and 17 colonies at dilution 10\u003csup\u003e5\u003c/sup\u003e.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eCultural Characteristics/ Morphology of Bacterial Isolates on Nutrient Agar\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cimg 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\"\u003e\u003c/strong\u003e\u003cbr\u003e\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eGrowth on MacConkey Agar\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eMacConkey agar is used to differentiate bacteria based on their ability to ferment lactose, and the results of the growth on these medium revealed distinct variations in lactose fermentation between the samples. Sample U exhibited moderate lactose fermentation at the highest dilution (10\u0026sup1;), with a pinkish mucoid appearance, which became less pronounced at lower dilutions. At dilution 10\u0026sup3;, lactose fermentation was minimal, and by dilution 10\u003csup\u003e5\u003c/sup\u003e, only slight fermentation was observed, indicating a lower capacity for lactose fermentation.\u003c/p\u003e\n\u003cp\u003eIn contrast, Sample P displayed much stronger lactose fermentation at all dilutions. At dilution 10\u0026sup1;, it showed a very pinkish color, indicating strong fermentation. The pinkish color remained prominent at dilution 10\u0026sup3;, though less intense, and at 10\u003csup\u003e5\u003c/sup\u003e, there was still noticeable fermentation, though reduced. These results suggest that Sample P has a more robust ability to ferment lactose compared to Sample U, which displayed weaker fermentation capabilities as dilution increased. This difference in lactose fermentation between the two samples could suggest variations in bacterial composition or metabolic activity.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eCultural Characteristics/ Morphology of Bacterial Isolates on MacConkey Agar\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cimg 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Characteristics/ Morphology of Bacterial Isolates on Eosin Methylene Blue (EMB) Agar and Salmonella Shigella Agar (SSA)\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eSubculturing on Eosin Methylene Blue (EMB) Agar and \u003cem\u003eSalmonella Shigella\u003c/em\u003e Agar (SSA) media provided additional insights into the microbial characteristics of Samples U and P. The results confirmed the presence of specific bacterial traits in both samples.\u003c/p\u003e\n\u003cp\u003eOn EMB agar, Sample U exhibited consistent purple mucoid colonies across all dilutions (10\u0026sup1;, 10\u0026sup3;, and 10\u003csup\u003e5\u003c/sup\u003e), which is indicative of \u003cem\u003eKlebsiella\u003c/em\u003e species and \u003cem\u003ecitrobacter\u003c/em\u003e species, known for their ability to ferment lactose and produce mucoid colonies. Similarly, Sample P also showed purple mucoid colonies at all dilutions, further suggesting the presence of lactose-fermenting bacteria, though the specific species was not identified.\u003c/p\u003e\n\u003cp\u003eBoth samples showed no growth on SSA agar at any dilution (10\u0026sup1;, 10\u0026sup3;, and 10\u003csup\u003e5\u003c/sup\u003e), which is indicative of the absence of \u003cem\u003eSalmonella\u003c/em\u003e or \u003cem\u003eShigella\u003c/em\u003e species, as these pathogens typically grow on SSA. The lack of growth on SSA could also imply that these samples do not harbor these pathogens under the conditions tested.\u003c/p\u003e\n\u003cp\u003eThese subculturing results reinforce the initial findings and provide more clarity on the microbial composition of the samples, highlighting the presence of \u003cem\u003eKlebsiella\u003c/em\u003e and \u003cem\u003ecitrobacter\u003c/em\u003e in both samples, but no indication of \u003cem\u003eSalmonella\u003c/em\u003e or \u003cem\u003eShigella\u003c/em\u003e contamination.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eCultural Characteristics/Morphology of Bacterial Isolates on Eosin Methylene Blue (EMB) Agar\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cimg 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\"\u003e\u003c/strong\u003e\u003cbr\u003e\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eBiochemical Tests\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eBiochemical tests were conducted to identify and characterize bacterial isolates from the samples. These tests evaluated oxidase activity, catalase production, and Gram staining results to help classify the microbes.\u003c/p\u003e\n\u003cp\u003eFor the \u003cstrong\u003eoxidase test\u003c/strong\u003e, both UATH and Psite samples (from EMB and MacConkey media) showed negative results, indicating that the bacteria do not produce oxidase. In the \u003cstrong\u003ecatalase test\u003c/strong\u003e, UATH isolates on EMB and Psite isolates on EMB were positive, forming bubbles, suggesting the presence of catalase. However, Psite isolates on MacConkey and UATH isolates on MacConkey were negative, showing no bubble formation. Lastly, \u003cstrong\u003eGram staining\u003c/strong\u003e revealed that both UATH and Psite isolates were Gram-negative, rod-shaped bacteria.\u003c/p\u003e\n\u003cp\u003eThese tests help confirm the bacterial characteristics of the isolates, providing insights into their classification.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eBiochemical Identification of Bacterial Isolate\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cimg src=\"data:image/png;base64,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\"\u003e\u003c/strong\u003e\u003cbr\u003e\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eKey\u003c/strong\u003e: Isolates, GR(Gram Reaction), Oxidase test, Catalase test, I(Indole test), VP(Voges-Proskauer), Citrate test, MR (Methyl-red test), PO (Probable Organism).\u003c/p\u003e\n\u003cp\u003e+ = positive\u003c/p\u003e\n\u003cp\u003e- = negative\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eAntibiotic Susceptibility Test\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eThe antibiotic susceptibility test was conducted using negative (-ve) discs to assess the resistance and susceptibility profiles of the bacterial isolates from UATH and Psite. The results are summarized in the table below\u003c/p\u003e\n\u003cp\u003eBoth UATH and Psite displayed uniform susceptibility across all antibiotics tested, including PEF(pefloxacin), CN(gentamicin), OFX(tarivid), SXT(septrin), AM(amoxacilin), CH(chloramphenicol), CPX(ciprofloxacin), S(streptomycin), SP(sparfloxacin), and AU(augmentin). This suggests that the isolates from both locations were susceptible to some of the antibiotics, indicating a broader effectiveness of these drugs against the bacterial strains present at both sites.\u003c/p\u003e\n\u003cp\u003eThese results highlight the consistent susceptibility and some resistance on negative discs using the CLSI guideline, however, indicates that there are antibiotics that may still be effective for treatment.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eAntibiotic Susceptibility profile of Gram Negative Bacterial Isolate\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cimg 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\"\u003e\u003c/strong\u003e\u003cbr\u003e\u003c/p\u003e\n\u003cp\u003eKey: Susceptible (S): The antibiotic is effective at inhibiting bacterial growth under standard testing conditions.\u003c/p\u003e\n\u003cp\u003eIntermediate (I): The organism shows partial susceptibility. This might indicate that the antibiotic could work at higher doses or under specific conditions.\u003c/p\u003e\n\u003cp\u003eResistant (R): The antibiotic is not effective in inhibiting the growth of the organism.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eAntibiotic Susceptibility Profile of Gram Negative Bacterial Isolates\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cimg 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\"\u003e\u003c/strong\u003e\u003cbr\u003e\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eKey\u003c/strong\u003e: Susceptible (S): The antibiotic is effective against the organism.\u003c/p\u003e\n\u003cp\u003eIntermediate (I): The organism has partial susceptibility, and higher doses of the antibiotic or different conditions might be required for efficacy.\u003c/p\u003e\n\u003cp\u003eResistant (R): The antibiotic is ineffective at inhibiting bacterial growth for this organism.\u003c/p\u003e"},{"header":"Discussion","content":"\u003cp\u003eThis study assessed the microbiological profiles and antibiotic susceptibility of bacterial isolates from borehole water samples collected from the University of Abuja Main Campus (Sample P) and the Teaching Hospital (Sample U). Sample P showed significantly higher bacterial loads across all media compared to Sample U, suggesting higher contamination levels. Variations in colony morphology and lactose fermentation further indicated differences in bacterial composition.\u003c/p\u003e\u003cp\u003eBiochemical tests identified catalase-positive, Gram-negative, rod-shaped bacteria likely of the \u003cem\u003eKlebsiella pnuemoniae\u003c/em\u003e and \u003cem\u003ecitrobacter\u003c/em\u003e genus, which are common in contaminated water and linked to various infections. This finding aligns with previous studies highlighting the health risks posed by \u003cem\u003eKlebsiella pnuemoniae\u003c/em\u003e and \u003cem\u003ecitrobacter genus\u003c/em\u003e contamination in water sources (Bassey \u003cem\u003eet al\u003c/em\u003e., 2017). The detection of antibiotic-resistant bacteria in borehole water is concerning and reflects global trends in antimicrobial resistance, as reported by Founou \u003cem\u003eet al\u003c/em\u003e. (2018).\u003c/p\u003e\u003cp\u003eAlthough pH levels for both samples were within the acceptable range (6.8–6.9), the elevated bacterial load in Sample P highlights the need for improved water management practices, including routine monitoring, sanitation, and disinfection. Similar recommendations have been made in previous studies on waterborne pathogens in Nigeria (Onuoha \u003cem\u003eet al\u003c/em\u003e., 2021; WHO, 2017).\u003c/p\u003e\u003cp\u003eThis study emphasizes the importance of monitoring borehole water quality and implementing effective treatment strategies to reduce public health risks, especially regarding bacterial contamination and antibiotic resistance (Ojo \u003cem\u003eet al\u003c/em\u003e., 2019).\u003c/p\u003e"},{"header":"Conclusion","content":"\u003cp\u003eThe study highlights critical differences in bacterial load and antibiotic susceptibility between the analyzed samples. Sample P demonstrated a higher microbial burden compared to Sample U, indicating a potentially greater risk of contamination in environments represented by Sample P. The pinkish mucoid colonies on MacConkey and purple mucoid colonies on EMB agar suggest the presence of lactose-fermenting bacteria such as \u003cem\u003eKlebsiella\u003c/em\u003e spp. These bacteria are known to be opportunistic pathogens. The study highlights the need for judicious antibiotic use and the adoption of robust infection control measures.\u003c/p\u003e"},{"header":"Recommendations","content":"\u003cp\u003eRegular microbiological screening of environmental and clinical samples should be carried out to detect emerging bacterial contaminants and antibiotic resistance trends.\u003c/p\u003e\u003cp\u003eStrict hygiene protocols should be enforced in environments associated with Sample P to reduce bacterial loads and prevent contamination.\u003c/p\u003e\u003cp\u003eInitiatives to regulate the use of antibiotics should be strengthened to prevent the escalation of resistant strains.\u003c/p\u003e\u003cp\u003eResearch into alternative antimicrobial agents, such as probiotics and natural antimicrobials, should be prioritized to combat resistant bacterial strains effectively.\u003c/p\u003e\u003cp\u003eGovernmental and non-governmental organizations should collaborate to improve water sanitation and hygiene in communities to minimize bacterial contamination risks\u003c/p\u003e"},{"header":"References","content":"\u003col\u003e\n\u003cli\u003eAdhikary, P. 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A nested case-control study of methemoglobinemia factors in children in Transylvania, Romania. \u003cem\u003eEnvironmental Health Perspectives, 110\u003c/em\u003e(8), 81-822.\u003c/li\u003e\n\u003c/ol\u003e"}],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":true,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":true,"hideJournal":true,"highlight":"","institution":"University of Abuja","isAcceptedByJournal":false,"isAuthorSuppliedPdf":false,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":false,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true},"keywords":"Bacteriological, Physicochemical, University of Abuja Teaching Hospital, University of Abuja Main Campus ","lastPublishedDoi":"10.21203/rs.3.rs-7640761/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-7640761/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003eThis study assessed the bacteriological and physicochemical properties of borehole water samples collected from the University of Abuja Main Campus and the University of Abuja Teaching Hospital. Serial dilution was conducted before the samples were cultured on Nutrient Agar, MacConkey Agar and sub-cultured on Eosin Methylene Blue (EMB) Agar, and \u003cem\u003eSalmonella-Shigella\u003c/em\u003e Agar (SSA) to reduce the bacterial load and determine the growth characteristics. Some growths were observed mainly lactose fermenting organism. Physicochemical parameters, including pH, temperature, turbidity, dissolved oxygen (DO), and heavy metals such as iron and manganese, were analyzed. Sample P exhibited higher microbial loads, with a mean colony forming unit count of 27.83, significantly higher than Sample U, which had a mean colony forming unit count of 3.17. Biochemical tests confirmed the isolates as Gram-negative, rod-shaped, bacilli, citrate-positive, oxidase negative, indole negative likely of the \u003cem\u003ecitrobacter\u003c/em\u003e genus. Oxidase negative, methyl-red negative, catalase-positive likely of the \u003cem\u003eklebsiella pneumoniae\u003c/em\u003e genus. Antibiotic susceptibility tests revealed that all the gram negative antibiotics were effective. Statistical analysis using one-way ANOVA showed significant differences (p\u0026thinsp;=\u0026thinsp;0.00025) in bacterial growth and physicochemical properties between the two sites. The pH ranged from 6.8 to 6.9, and turbidity values were higher in Sample P, indicating potential health risks. The findings underscore the importance of regular water quality monitoring, effective antibiotic use, and robust infection control strategies to mitigate public health risks associated with waterborne pathogens. The higher bacterial burden and contamination risk observed in Sample P highlight the urgent need for targeted interventions to ensure safe and sustainable water sources.\u003c/p\u003e","manuscriptTitle":"Bacteriological and Physicochemical Parameters of Borehole Water in University of Abuja Main Campus and University of Abuja Teaching Hospital","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2025-09-18 07:34:26","doi":"10.21203/rs.3.rs-7640761/v1","editorialEvents":[{"type":"communityComments","content":0}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"9a78343d-9657-4cc7-bb03-65828eb9f6ed","owner":[],"postedDate":"September 18th, 2025","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"posted","subjectAreas":[{"id":54886276,"name":"Applied \u0026 Industrial Microbiology"},{"id":54886277,"name":"General Microbiology"},{"id":54886278,"name":"Applied Biochemistry"}],"tags":[],"updatedAt":"2025-09-18T07:34:27+00:00","versionOfRecord":[],"versionCreatedAt":"2025-09-18 07:34:26","video":"","vorDoi":"","vorDoiUrl":"","workflowStages":[]},"version":"v1","identity":"rs-7640761","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-7640761","identity":"rs-7640761","version":["v1"]},"buildId":"8U1c8b4HqxoKbykW_rLl7","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}