Comparative membrane proteomic analysis of Tritrichomonas foetus isolates

Comparative membrane proteomic analysis of Tritrichomonas foetus isolates | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Article Comparative membrane proteomic analysis of Tritrichomonas foetus isolates Maria B. Rivero, Andrés M. Alonso, Maria E. Abdala, Melchor E. Luque, and 3 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-4118738/v1 This work is licensed under a CC BY 4.0 License Status: Published Journal Publication published 24 Jul, 2024 Read the published version in Scientific Reports → Version 1 posted 10 You are reading this latest preprint version Abstract Tritrichomonas foetus is a flagellated and anaerobic parasite able to infect cattle and felines. Despite its prevalence, there is no effective standardized or legal treatment for T. foetus -infected cattle; the vaccination still has limited success in mitigating infections and reducing abortion risk; and nowadays, the diagnosis of T. foetus presents important limitations in terms of sensitivity and specificity in bovines. Here, we characterize the plasma membrane proteome of T. foetus and identify proteins that are represented in different isolates of this protozoan. Additionally, we performed a bioinformatic analysis that revealed the antigenicity potential of some of those proteins. This analysis is the first study to identify common proteins at the plasma membrane of different T. foetus isolates that could be targets for alternative diagnostic or vaccine techniques in the future. Biological sciences/Biotechnology Biological sciences/Microbiology Tritrichomonas foetus isolates membrane proteins diagnosis Figures Figure 1 Figure 2 Figure 3 Figure 4 Figure 5 Full Text Additional Declarations No competing interests reported. Supplementary Files TableS1.xls Cite Share Download PDF Status: Published Journal Publication published 24 Jul, 2024 Read the published version in Scientific Reports → Version 1 posted Editorial decision: Revision requested 04 May, 2024 Reviews received at journal 29 Apr, 2024 Reviews received at journal 19 Apr, 2024 Reviewers agreed at journal 09 Apr, 2024 Reviewers agreed at journal 09 Apr, 2024 Reviewers invited by journal 09 Apr, 2024 Editor assigned by journal 09 Apr, 2024 Editor invited by journal 05 Apr, 2024 Submission checks completed at journal 05 Apr, 2024 First submitted to journal 17 Mar, 2024 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-4118738","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Article","associatedPublications":[],"authors":[{"id":289382189,"identity":"ddfcf69e-541b-4ada-9182-7327d6a75868","order_by":0,"name":"Maria B. Rivero","email":"","orcid":"","institution":"Instituto Multidisciplinario de Salud, Tecnología y Desarrollo (IMSaTeD)","correspondingAuthor":false,"prefix":"","firstName":"Maria","middleName":"B.","lastName":"Rivero","suffix":""},{"id":289382190,"identity":"498bbaa8-d3c7-41b0-b6c6-073cfabf7fc7","order_by":1,"name":"Andrés M. Alonso","email":"","orcid":"","institution":"Instituto Tecnológico de Chascomús","correspondingAuthor":false,"prefix":"","firstName":"Andrés","middleName":"M.","lastName":"Alonso","suffix":""},{"id":289382191,"identity":"9b21d08e-2242-4c28-a964-a1c6a1395018","order_by":2,"name":"Maria E. Abdala","email":"","orcid":"","institution":"Instituto Multidisciplinario de Salud, Tecnología y Desarrollo (IMSaTeD)","correspondingAuthor":false,"prefix":"","firstName":"Maria","middleName":"E.","lastName":"Abdala","suffix":""},{"id":289382192,"identity":"f042d699-1666-4b45-9fac-1c4eef636363","order_by":3,"name":"Melchor E. 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Coceres","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAAA3klEQVRIiWNgGAWjYBADOTYQydhAghZjNgZmErUkNhCthb+9+eHjioq69D7p/oMPfu5gsOuXSGD8XIFHi8SZY8aGZ84czm2TOcxs2HuGIXnmjARmyTN4tBhI5LBJNrYdyG2TSGaTZmxjSDY4c4BBEp8DgVrYfzb+q0tnk0hm/w3VwvyTgBY2xsYG5gSgFjZmoBY7g+MNbHhtAflFsuHYYUOgw4wle9skEiTbG9ss8WkBhdjHhpo6efkZiQ8//GyzsednZj58E58WDFuBEURShAKBPWnKR8EoGAWjYCQAAG27RrsCYJqFAAAAAElFTkSuQmCC","orcid":"","institution":"Instituto Tecnológico de Chascomús","correspondingAuthor":true,"prefix":"","firstName":"Veronica","middleName":"M.","lastName":"Coceres","suffix":""},{"id":289382195,"identity":"3cf02c85-6758-4b6e-8cd4-91ee98a03739","order_by":6,"name":"Fernando D. Rivero","email":"","orcid":"","institution":"Instituto Multidisciplinario de Salud, Tecnología y Desarrollo (IMSaTeD)","correspondingAuthor":false,"prefix":"","firstName":"Fernando","middleName":"D.","lastName":"Rivero","suffix":""}],"badges":[],"createdAt":"2024-03-17 21:18:57","currentVersionCode":1,"declarations":"","doi":"10.21203/rs.3.rs-4118738/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-4118738/v1","draftVersion":[],"editorialEvents":[{"content":"https://doi.org/10.1038/s41598-024-67827-8","type":"published","date":"2024-07-24T16:16:03+00:00"}],"editorialNote":"","failedWorkflow":false,"files":[{"id":54451016,"identity":"bfcc794c-4a07-4969-8b1c-889cbd7e8e2a","added_by":"auto","created_at":"2024-04-10 17:55:48","extension":"jpg","order_by":1,"title":"Figure 1","display":"","copyAsset":false,"role":"figure","size":96227,"visible":true,"origin":"","legend":"\u003cp\u003eSubcellular fractionation of T. foetus. 1: Pellets of T. foetus isolates (Tf0, Tf1, Tf2, Tf3, Tf4, and Tf5) were disrupted mechanically. 2: PNS (post-nuclear supernatant) obtained was centrifuged, yielding pellet 1 (f1) and supernatant 1 (SN1). 3: Successive centrifugations at 10,000 g, at 30,000 g at 60,000 g, and 100,000 g. Pellets 1-5= f1-f5. Supernants 2-5= SN1-5\u003c/p\u003e","description":"","filename":"1.jpg","url":"https://assets-eu.researchsquare.com/files/rs-4118738/v1/892f22cf540261c1560a2544.jpg"},{"id":54451015,"identity":"209e6ed1-7fe0-433c-be5d-91030ef28975","added_by":"auto","created_at":"2024-04-10 17:55:48","extension":"jpg","order_by":2,"title":"Figure 2","display":"","copyAsset":false,"role":"figure","size":222067,"visible":true,"origin":"","legend":"\u003cp\u003eRelative abundance of transmembrane proteins (TMP) along membrane fractions (f1-f5) from T. foetus isolate 2 (Tf2). A Kruskal-Wallis test was implemented for comparing means; ***: p-value \u0026lt;= 0.001; ****: p-value \u0026lt;= 0.0001. rAb = relative abundance.\u003c/p\u003e","description":"","filename":"2.jpg","url":"https://assets-eu.researchsquare.com/files/rs-4118738/v1/2fbf2c758c13b765b664c59b.jpg"},{"id":54451018,"identity":"203a1300-6e39-4cf2-9e47-fe7e7e25e2d4","added_by":"auto","created_at":"2024-04-10 17:55:49","extension":"jpg","order_by":3,"title":"Figure 3","display":"","copyAsset":false,"role":"figure","size":863895,"visible":true,"origin":"","legend":"\u003cp\u003eIndirect immunofluorescence of T. foetus isolates (Tf0-Tf5) was performed with antibodies obtained with f2 and f3 from isolate Tf2 (at a dilution of 1:100) and incubated with the secondary antibody Anti-Mouse Polyvalent Immunoglobulins-FITC at a dilution of 1:1000 (green). PC: phase contrast. DAPI (nuclei).\u003c/p\u003e","description":"","filename":"3.jpg","url":"https://assets-eu.researchsquare.com/files/rs-4118738/v1/d3c6a40a88856e34331aa78f.jpg"},{"id":54451019,"identity":"17696b9e-26b0-4650-9ca5-0543d15b32eb","added_by":"auto","created_at":"2024-04-10 17:55:49","extension":"jpg","order_by":4,"title":"Figure 4","display":"","copyAsset":false,"role":"figure","size":584035,"visible":true,"origin":"","legend":"\u003cp\u003eA. Principal component analysis (PCA) for fractions enriched in proteins with at least one transmembrane domain of T. foetus isolates; B. Heat map of proteins with at least one transmembrane domain present in T. foetus isolates. The heat map was sectioned into five groups or clusters of proteins based on their abundance (log2(rAb))\u003c/p\u003e","description":"","filename":"4.jpg","url":"https://assets-eu.researchsquare.com/files/rs-4118738/v1/6f6ee143e3bd16c89a4a71ff.jpg"},{"id":54451020,"identity":"f076b6ee-bf18-486b-847c-603c2376e004","added_by":"auto","created_at":"2024-04-10 17:55:49","extension":"jpg","order_by":5,"title":"Figure 5","display":"","copyAsset":false,"role":"figure","size":328320,"visible":true,"origin":"","legend":"\u003cp\u003eA. Molecular function enrichment analysis for cluster 1*, cluster 2**, and cluster 4****. B. Trend graph for each protein with at least one transmembrane domain integrating cluster 1. Those proteins with similar abundance (log2(rAb)) in each isolate are highlighted.\u003c/p\u003e","description":"","filename":"5.jpg","url":"https://assets-eu.researchsquare.com/files/rs-4118738/v1/c163cff90c8817dcbe2cdb3e.jpg"},{"id":61596223,"identity":"be044b66-8090-4d7e-b914-5037926f169b","added_by":"auto","created_at":"2024-08-01 17:25:49","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":2480413,"visible":true,"origin":"","legend":"","description":"","filename":"MS.pdf","url":"https://assets-eu.researchsquare.com/files/rs-4118738/v1_covered_4fc9dc86-8655-4bc6-83cf-51f375de6fbf.pdf"},{"id":54451017,"identity":"e67c06d7-8151-477e-8b50-b97a76957acd","added_by":"auto","created_at":"2024-04-10 17:55:48","extension":"xls","order_by":0,"title":"","display":"","copyAsset":false,"role":"supplement","size":111104,"visible":true,"origin":"","legend":"","description":"","filename":"TableS1.xls","url":"https://assets-eu.researchsquare.com/files/rs-4118738/v1/b5af250223315f2dc8993d1a.xls"}],"financialInterests":"No competing interests reported.","formattedTitle":"Comparative membrane proteomic analysis of Tritrichomonas foetus isolates","fulltext":[],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":false,"highlight":"","institution":"","isAcceptedByJournal":true,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":true,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"scientific-reports","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":false,"externalIdentity":"scirep","sideBox":"Learn more about [Scientific Reports](http://www.nature.com/srep/)","snPcode":"","submissionUrl":"","title":"Scientific Reports","twitterHandle":"","acdcEnabled":true,"dfaEnabled":true,"editorialSystem":"stoa","reportingPortfolio":"Scientific Reports","inReviewEnabled":true,"inReviewRevisionsEnabled":true},"keywords":"Tritrichomonas foetus isolates, membrane proteins, diagnosis","lastPublishedDoi":"10.21203/rs.3.rs-4118738/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-4118738/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003e\u003cem\u003eTritrichomonas foetus\u003c/em\u003e is a flagellated and anaerobic parasite able to infect cattle and felines. Despite its prevalence, there is no effective standardized or legal treatment for \u003cem\u003eT. foetus\u003c/em\u003e-infected cattle; the vaccination still has limited success in mitigating infections and reducing abortion risk; and nowadays, the diagnosis of \u003cem\u003eT. foetus\u003c/em\u003e presents important limitations in terms of sensitivity and specificity in bovines. Here, we characterize the plasma membrane proteome of \u003cem\u003eT. foetus\u003c/em\u003e and identify proteins that are represented in different isolates of this protozoan. Additionally, we performed a bioinformatic analysis that revealed the antigenicity potential of some of those proteins. 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