Genotype-specific responses to drought stress in Siberian wildrye (Elymus sibiricus): Insights from comparative physiological and transcriptomic analyses

Genotype-specific responses to drought stress in Siberian wildrye (Elymus sibiricus): Insights from comparative physiological and transcriptomic analyses | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Article Genotype-specific responses to drought stress in Siberian wildrye (Elymus sibiricus): Insights from comparative physiological and transcriptomic analyses Yongping An, Qian Wang, Yannong Cui, Xin Liu, Ping Wang, Yue Zhou, and 5 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-4251832/v1 This work is licensed under a CC BY 4.0 License Status: Published Journal Publication published 10 Sep, 2024 Read the published version in Scientific Reports → Version 1 posted 11 You are reading this latest preprint version Abstract Siberian wildrye (Elymus sibiricus) is a xero-mesophytic forage grass with high nutritional quality and stress tolerance. Among its numerous germplasm resources, some possess superior drought resistance. In this study, we investigated the physiological differences between drought-tolerant genotype (DT) and drought-sensitive genotype (DS) leaves under drought stress. The results showed that under drought stress, DT maintained a lower leaf water potential for water absorption, sustained higher photosynthetic efficiency, and reduced oxidative damage in leaves by efficiently maintaining the ascorbic acid-glutathione (ASA-GSH) cycle to scavenge reactive oxygen species (ROS) compared to DS. Unigene0047636 (CER1) may positively regulates the synthesis of very-long-chain (VLC) alkanes in cuticular wax biosynthesis, influencing plant responses to abiotic stresses. Correspondingly, wax monomers content showed significant induction by osmotic stress in DT but not in DS. It is suggested that limiting stomatal and cuticle transpiration under drought stress to maintain higher photosynthetic efficiency and water use efficiency (WUE) is one of the critical mechanisms that confer stronger drought resistance to DT. This study provides new insights into the molecular mechanisms underlying drought tolerance in E. sibiricus. The identified genes may provide a foundation for the selection and breeding of drought-tolerant crops. Biological sciences/Plant sciences Biological sciences/Plant sciences/Plant molecular biology Biological sciences/Plant sciences/Plant physiology Biological sciences/Plant sciences/Plant stress responses Siberian wildrye osmotic resistance differentially expressed genes (DEGs) stress tolerance ROS ABA cuticular wax Full Text Additional Declarations No competing interests reported. Supplementary Files Supplementary.zip Cite Share Download PDF Status: Published Journal Publication published 10 Sep, 2024 Read the published version in Scientific Reports → Version 1 posted Editorial decision: Revision requested 20 Jun, 2024 Reviews received at journal 21 May, 2024 Reviewers agreed at journal 20 May, 2024 Reviews received at journal 17 May, 2024 Reviewers agreed at journal 09 May, 2024 Reviewers agreed at journal 09 May, 2024 Reviewers invited by journal 04 May, 2024 Editor assigned by journal 28 Apr, 2024 Editor invited by journal 18 Apr, 2024 Submission checks completed at journal 18 Apr, 2024 First submitted to journal 11 Apr, 2024 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-4251832","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Article","associatedPublications":[],"authors":[{"id":293721246,"identity":"58388cbb-ce48-4b14-bffb-fc6405f2201b","order_by":0,"name":"Yongping An","email":"","orcid":"","institution":"Sichuan Zoige Alpine Wetland Ecosystem National Observation and Research Station, Southwest Minzu University","correspondingAuthor":false,"prefix":"","firstName":"Yongping","middleName":"","lastName":"An","suffix":""},{"id":293721247,"identity":"2ff0bf39-7fac-44fe-950d-bd7395d9b14a","order_by":1,"name":"Qian 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