Preliminary study on the construction of animal model for non-puerperal mastitis

Abstract Objection: This qualitative study explored the key factors affecting the occurrence of non-puerperal mast (NPM） and the construction of an animal model which is closer to the characteristics of the disease.Background: The etiological factors and pathogenesis of NPM are unknown, and the incidence, misdiagnosis rate and recurrence rate are high. Previously reported modeling methods vary, reproducibility is poor, and disease characteristics cannot be well characterized, so new animal models are urgently needed for research.Methods: In this study, using female Wistar rats to construct a non-puerperal mastitis animal model by intraperitoneal injection of metoclopramide to simulate hyperprolactinemia + autologous milk injected into the mammary gland to simulate ductal secretions. Results: Pathological results showed that 2 weeks after injection in group C (intraperitoneal injection of metoclopramide for 2 weeks + breast injection of maternal milk mixture twice) and group D (intraperitoneal injection of metoclopramide for 2 weeks + breast injection of maternal milk mixture three times), lipid stasis, periductal inflammation, and massive plasma cell and lymphocyte infiltration were observed,similar to the lesions in patients with human plasma cell mastitis. Conclusions: We believe that we can can establish a non-puerperal mastitis model by altering prolactin levels and ductal secretion stasis in rats.