Evaluation of the Ovsynch and Co-synch protocols on estrus intensity and pregnancy rate in Anatolian buffalo heifers

Evaluation of the Ovsynch and Co-synch protocols on estrus intensity and pregnancy rate in Anatolian buffalo heifers | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Evaluation of the Ovsynch and Co-synch protocols on estrus intensity and pregnancy rate in Anatolian buffalo heifers ilktan Baştan, Fırat Korkmaz, Derya Şahin, Seher Şimşek, Mehmet Ali Yılmaz This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-3925985/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract This study aimed to evaluate the Ovsynch and Co-synch synchronization protocols in terms of estrus intensity and pregnancy rate in Anatolian buffalo heifers. To this end, 27 Anatolian buffalo heifers were used. For the Ovsynch synchronization protocol, GnRH (buserelin acetate, 12 μg) was administered on day 0, PGF2α (cloprostenol, 500 μg) on day 7, and GnRH (buserelin acetate, 12 μg) on day 9, and artificial insemination was performed 16-20 hours after the second GnRH application. For the Co-synch protocol, GnRH (buserelin acetate, 12 μg) was administered on day 0, PGF2α (cloprostenol, 500 μg) on day 7, and GnRH (buserelin acetate, 12 μg) along with artificial insemination on day 10. At the time of artificial insemination, estrus intensity was classified as intense, moderate, weak, or negative. Pregnancies were within 40 to 70 days after artificial insemination using pregnancy-related glycoprotein or ultrasonography examinations. Intense, moderate, weak, and negative estrus intensities were determined at rates of 20%, 20%, 26.6%, and 33.3%, respectively, in the Ovsynch group and 41.6%, 58.3%, 0%, and 0%, respectively, in the Co-synch group (P<0.05). Pregnancy rates were 30% and 75% in the Ovsynch and Cosynch groups, respectively (P<0.05). In conclusion, the Co-synch protocol was found to be more successful than the Ovsynch protocol for synchronization in Anatolian buffalo heifers. In addition, to increase the success of artificial insemination, it is recommended to perform cervix uteri monitoring and perform artificial insemination only on animals showing estrous behavior. artificial insemination Bubalus bubalis estrus response monitoring of cervix uteri synchronization Full Text Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-3925985","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":271843892,"identity":"91b82674-70e1-40bd-bd69-99d0eee8b0d9","order_by":0,"name":"ilktan Baştan","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAABE0lEQVRIiWNgGAWjYBACNgkILcfAwANmGLAxH4Cx8WgBqjGGKEsAamFLALMlcOqBaklsgGlhIKSFT7r58ecPNXfS17b3Ht3w8YedMR8bA+ODt20MdeYNOBwmc8xM4sCxZ7nbzpxLuzkjIdmMjY2B2XBuG4OEzAFcfkkwYzjAdjh3240cs9s8Ccw2bPINbNK8QC24XMYmkf75w4F/h9PN7r8xu/0nod4GaAv7b/xacgwkDrYdTjC7wWN2myHhMMhhbMx4tcicKZM423fYcNuZHLObPWnHjdnYGJsl55yTkJyBQ4v87PbNHyq+HZY3O37G7MYPm2rD+W3MBz+8KbPhxx0xmICxgQFfTI6CUTAKRsEoIAwA5QRWy1FVSUkAAAAASUVORK5CYII=","orcid":"https://orcid.org/0000-0001-8155-1960","institution":"Mehmet Akif Ersoy University: Burdur Mehmet Akif Ersoy Universitesi","correspondingAuthor":true,"prefix":"","firstName":"ilktan","middleName":"","lastName":"Baştan","suffix":""},{"id":271843893,"identity":"61c19cc1-842a-4a0e-80a7-8576a1a1e2a1","order_by":1,"name":"Fırat Korkmaz","email":"","orcid":"","institution":"Mehmet Akif Ersoy University: Burdur Mehmet Akif Ersoy Universitesi","correspondingAuthor":false,"prefix":"","firstName":"Fırat","middleName":"","lastName":"Korkmaz","suffix":""},{"id":271843894,"identity":"a81a7f0b-4b2f-457f-9146-adc5b19eb4b2","order_by":2,"name":"Derya Şahin","email":"","orcid":"","institution":"Republic of Turkey Ministry of Agriculture and Forestry: Turkiye Cumhuriyeti Tarim ve Orman Bakanligi","correspondingAuthor":false,"prefix":"","firstName":"Derya","middleName":"","lastName":"Şahin","suffix":""},{"id":271843895,"identity":"b5b6fdcf-917b-4e99-8d57-615172954739","order_by":3,"name":"Seher Şimşek","email":"","orcid":"","institution":"Republic of Turkey Ministry of Agriculture and Forestry: Turkiye Cumhuriyeti Tarim ve Orman Bakanligi","correspondingAuthor":false,"prefix":"","firstName":"Seher","middleName":"","lastName":"Şimşek","suffix":""},{"id":271843896,"identity":"eb33cea6-97ac-4b9d-a99d-c1d9203a16a6","order_by":4,"name":"Mehmet Ali Yılmaz","email":"","orcid":"","institution":"Republic of Turkey Ministry of Agriculture and Forestry: Turkiye Cumhuriyeti Tarim ve Orman Bakanligi","correspondingAuthor":false,"prefix":"","firstName":"Mehmet","middleName":"Ali","lastName":"Yılmaz","suffix":""}],"badges":[],"createdAt":"2024-02-04 03:03:26","currentVersionCode":1,"declarations":"","doi":"10.21203/rs.3.rs-3925985/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-3925985/v1","draftVersion":[],"editorialEvents":[],"editorialNote":"","failedWorkflow":false,"files":[{"id":53975531,"identity":"5eb0415d-c42d-4987-b6d3-db33e0f2b6b8","added_by":"auto","created_at":"2024-04-03 00:21:22","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":418648,"visible":true,"origin":"","legend":"","description":"","filename":"ilktanBastanetal..pdf","url":"https://assets-eu.researchsquare.com/files/rs-3925985/v1_covered_f924fec3-0dfc-4b0b-8721-9b0c518df751.pdf"}],"financialInterests":"","formattedTitle":"Evaluation of the Ovsynch and Co-synch protocols on estrus intensity and pregnancy rate in Anatolian buffalo heifers","fulltext":[],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":true,"highlight":"","institution":"","isAcceptedByJournal":false,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":true,"isPdf":true,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true},"keywords":"artificial insemination, Bubalus bubalis, estrus response, monitoring of cervix uteri, synchronization","lastPublishedDoi":"10.21203/rs.3.rs-3925985/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-3925985/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"This study aimed to evaluate the Ovsynch and Co-synch synchronization protocols in terms of estrus intensity and pregnancy rate in Anatolian buffalo heifers. To this end, 27 Anatolian buffalo heifers were used. For the Ovsynch synchronization protocol, GnRH (buserelin acetate, 12 μg) was administered on day 0, PGF2α (cloprostenol, 500 μg) on day 7, and GnRH (buserelin acetate, 12 μg) on day 9, and artificial insemination was performed 16-20 hours after the second GnRH application. For the Co-synch protocol, GnRH (buserelin acetate, 12 μg) was administered on day 0, PGF2α (cloprostenol, 500 μg) on day 7, and GnRH (buserelin acetate, 12 μg) along with artificial insemination on day 10. At the time of artificial insemination, estrus intensity was classified as intense, moderate, weak, or negative. Pregnancies were within 40 to 70 days after artificial insemination using pregnancy-related glycoprotein or ultrasonography examinations. Intense, moderate, weak, and negative estrus intensities were determined at rates of 20%, 20%, 26.6%, and 33.3%, respectively, in the Ovsynch group and 41.6%, 58.3%, 0%, and 0%, respectively, in the Co-synch group (P\u0026lt;0.05). Pregnancy rates were 30% and 75% in the Ovsynch and Cosynch groups, respectively (P\u0026lt;0.05). In conclusion, the Co-synch protocol was found to be more successful than the Ovsynch protocol for synchronization in Anatolian buffalo heifers. 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