Establishment of Agrobacterium-mediated genetic transformation in Salix brachista.

Establishment of Agrobacterium-mediated genetic transformation in Salix brachista. | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Establishment of Agrobacterium-mediated genetic transformation in Salix brachista. Dan Tang, Yumeng Li, García-Caparrós Pedro, Jiahui Chen, Xudong Sun This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-6446817/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Salix brachista, commonly known as the Cushion willow, is a plant species that is indigenous to the alpine ice edge zones. This remarkable plant is predominantly found in the Hengduan Mountains. As a quintessential example of an alpine plant, the development of a genetic transformation system for Salix brachista is of paramount importance for unraveling the origins and the intricate mechanisms that underpin the diversity of alpine plant species. In the course of our research, we initially focused on creating a robust regeneration system for this species, utilizing stem segments of Salix brachista. Through meticulous optimization of various factors, we achieved a commendable average regeneration rate of 52%. Building upon this foundational work, we then established a straightforward and highly effective genetic transformation protocol, employing the Agrobacterium-mediated method. The successful establishment of this genetic transformation method not only represents a significant advancement in itself but also opens up new avenues for further gene editing and functional research endeavors in Salix brachista. Salix brachista Genetic transformation FT Flowering Full Text Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-6446817","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":449116608,"identity":"27ec2cad-2b2e-4b71-9de9-2e95d9d30760","order_by":0,"name":"Dan Tang","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAAAzklEQVRIie3RsQrCMBCA4SuBuBy6BpT6BEKkIBSkfZWIax+gY6XQTXyBgg8hdD6XThXXjnZx6mA3N42bTo2bYP45X3JHAGy2H4wPdjdS8QPDc0pmZIg0p0vFJlCXyoy4QnnHJmNLoEgaDgZKkao4OknV1S0E7izpJUR6F0TGtgc/h7W3oD7ibBL9ikAOp2KMQKuilzCmj2USEaKrIeH8RRQKiLgh0dfrwQilKD0/lwa7TPct6+4xhXKUNnUbB24v+Uig4de8k2+FzWaz/UVPjgVDgpfDhycAAAAASUVORK5CYII=","orcid":"https://orcid.org/0009-0009-6908-5982","institution":"Kunming Institute of Botany Chinese Academy of Sciences","correspondingAuthor":true,"prefix":"","firstName":"Dan","middleName":"","lastName":"Tang","suffix":""},{"id":449116609,"identity":"bb467ec3-f920-423b-9f64-dcd7421f1e74","order_by":1,"name":"Yumeng Li","email":"","orcid":"","institution":"Kunming Institute of Botany Chinese Academy of Sciences","correspondingAuthor":false,"prefix":"","firstName":"Yumeng","middleName":"","lastName":"Li","suffix":""},{"id":449116610,"identity":"f53f651f-4137-4757-b45c-e945fef4376d","order_by":2,"name":"García-Caparrós Pedro","email":"","orcid":"","institution":"University of Almeria: Universidad de Almeria","correspondingAuthor":false,"prefix":"","firstName":"García-Caparrós","middleName":"","lastName":"Pedro","suffix":""},{"id":449116611,"identity":"5156b4d2-506d-46a0-a2f8-2127ed2f6a24","order_by":3,"name":"Jiahui Chen","email":"","orcid":"","institution":"Kunming Institute of Botany Chinese Academy of Sciences","correspondingAuthor":false,"prefix":"","firstName":"Jiahui","middleName":"","lastName":"Chen","suffix":""},{"id":449116612,"identity":"3cd08e34-208a-4813-a955-4faa77c84269","order_by":4,"name":"Xudong Sun","email":"","orcid":"https://orcid.org/0000-0002-2434-8031","institution":"Kunming Institute of Botany Chinese Academy of Sciences","correspondingAuthor":false,"prefix":"","firstName":"Xudong","middleName":"","lastName":"Sun","suffix":""}],"badges":[],"createdAt":"2025-04-14 14:09:08","currentVersionCode":1,"declarations":"","doi":"10.21203/rs.3.rs-6446817/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-6446817/v1","draftVersion":[],"editorialEvents":[],"editorialNote":"","failedWorkflow":false,"files":[{"id":82512071,"identity":"c666b605-946e-4ff4-ad60-3f09501c89d7","added_by":"auto","created_at":"2025-05-12 10:53:49","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":514106,"visible":true,"origin":"","legend":"","description":"","filename":"ManuscriptRevised20250416.pdf","url":"https://assets-eu.researchsquare.com/files/rs-6446817/v1_covered_3573b719-a309-4646-b2e1-874792b9f527.pdf"}],"financialInterests":"","formattedTitle":"Establishment of Agrobacterium-mediated genetic transformation in Salix brachista.","fulltext":[],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":true,"highlight":"","institution":"","isAcceptedByJournal":false,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":true,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true},"keywords":"Salix brachista, Genetic transformation, FT, Flowering","lastPublishedDoi":"10.21203/rs.3.rs-6446817/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-6446817/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"Salix brachista, commonly known as the Cushion willow, is a plant species that is indigenous to the alpine ice edge zones. This remarkable plant is predominantly found in the Hengduan Mountains. As a quintessential example of an alpine plant, the development of a genetic transformation system for Salix brachista is of paramount importance for unraveling the origins and the intricate mechanisms that underpin the diversity of alpine plant species. In the course of our research, we initially focused on creating a robust regeneration system for this species, utilizing stem segments of Salix brachista. Through meticulous optimization of various factors, we achieved a commendable average regeneration rate of 52%. Building upon this foundational work, we then established a straightforward and highly effective genetic transformation protocol, employing the Agrobacterium-mediated method. 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