Exogenous plant growth regulator and foliar fertilizers for phytoextraction of cadmium with Boehmeria nivea [L.] Gaudich from contaminated field soil.

Exogenous plant growth regulator and foliar fertilizers for phytoextraction of cadmium with Boehmeria nivea [L.] Gaudich from contaminated field soil. | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Exogenous plant growth regulator and foliar fertilizers for phytoextraction of cadmium with Boehmeria nivea [L.] Gaudich from contaminated field soil. Wenxian peng, Yejun He, Si He, Jingfeng Luo, Yi Zeng, Xiaoyang Zhang, and 3 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-1197456/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract (1) Background: As a enrichment plant, ramie can be used for the phytoremediation of cadmium (Cd)-contaminated soil. However, it is worth exploring the role of plant growth regulators and foliar fertilizers in the process of plant growth and development and Cd adsorption. (2) Methods: By measuring the agronomic traits, Cd content of aboveground and underground ramie, calculating the Cd transfer coefficient (TF) and Cd bioconcentration factors (BCF), and the correlation between various indicators. This study examined the effects of plant growth regulators and foliar fertilizers on ramie’s capacity for Cd accumulation and transportation, (3) Results: Plant growth regulators and foliar fertilizers increased the Cd content of the aboveground ramie, reduced the Cd content of the underground ramie, and increased the TF. Among them, GA-1 increased the Cd content of the aboveground ramie to 3 times more than that of the control and reduced the Cd content of the underground ramie by 54.76%. Salicylic acid (SA) increased the Cd content of the aboveground ramie to 3 times more than that of the control. The combination of GA and foliar fertilizer reduced the Cd content of the aboveground and underground ramie and the TF and BCF of the underground ramie. After the hormones were sprayed, the TF of ramie had a significant positive correlation with the Cd content of the aboveground ramie; the BCF of the aboveground ramie had a significant positive correlation with the Cd content and TF of the aboveground ramie. (4) Conclusions: The results indicate that Brassinolide (BR), gibberellin (GA), ethephon (ETH), polyamines (PAs), and salicylic acid (SA) have different effects on the enrichment and transport of Cd in ramie. This study provided an effective method to improve the capacity for ramie to adsorb heavy metals during cultivation. Ramie plant growth regulator Fertilizer Cadmium Gibberellin Phytoextraction Figures Figure 1 Figure 2 Figure 3 Figure 4 Figure 5 Figure 6 Figure 7 Figure 8 1. Introduction With the advances in urbanization and industrialization, heavy metal pollution in China has become a serious problem. (Cai et al. 2019 , Huang et al. 2017b , Zeng et al. 2017 ) According to a 2014 survey of soil pollution in China, 16.1% of the country’s soil contamination exceeded the legal limit, with heavy metal pollution contributing the most (82%). Cadmium (Cd) is a nonbiological essential heavy metal and one of the most toxic components of heavy metal pollution.(Koleli et al. 2004 ) Approximately 20% of total cultivated lands were contaminated with Cd in China.(Xue et al. 2014 ) Because of its similar chemical structure to zinc, Cadmium is easily absorbed by plants and can cause serious toxicity to plants, animals and humans even at low concentrations (Malandrino et al. 2011 ) Cadmium in soil can reduce plant growth, biomass, crop yield, and quality in plant(Ramzani et al. 2016 ) The ecotoxicity of Cadmium is relatively large, and the impact on the environment and the population health is more worthy of attention and the increasing population and decreasing farmland area seriously threaten the security of food, fiber, and so on. Hence, remediate and reuse the Cd-polluted lands is becoming more and more necessary and urgent. And there is a critical need to develop efficient techniques to remediate soil contaminated with Cd. Phytoremediation is a method of remediation that considers both ecological and economic effects and is a green technology developed for its strong potential to remove environmental pollution. (Huang et al. 2017a , Yang &Shen 2020 , Zhu et al. 2020 ) It is often used in large-scale decontamination projects and has attracted considerable attention in recent years(Liang et al. 2021 ) Phytoremediation can mainly be categorized into phytostabilization, photoevaporation, and phytoextraction according to the uptake mechanisms. Phytoextraction is considered more effective because it can permanently remove metals from contaminated sites. The repair ability of this plant depends not only on its biomass, but also on its absorptive capacity(Zhao et al. 2021 ). Ramie ( Boehmeria nivea [L.] Gaudich.) is a highly adaptable herbaceous perennial root that has been cultivated for more than 2000 years in China.(Tang et al. 2015b ) Its fast growth, high fecundity, and high biological yield(Ali &Hadi 2015 ) make up for the deficiencies of other hyperaccumulators, such as Sedum alfredii (Tao et al. 2020 ) and sunflowers(Zamani et al. 2020 ). Research has demonstrated that ramie can remove a fair amount of metal from the soils due to its stronger root system, faster growth rate, and higher biomass(Yang et al. 2010 ). Ramie is commonly used for its fiber, its products do not enter the food chain, and it is not associated with any health risk. Researchers have also modified varieties of ramie at the genetic level to improve its tolerance and ability to accumulate heavy metals.(Zhu et al. 2020 ) Moreover, ramie is a permanent crop that provides ecological and economic benefits to cultivation measures, and the cost of restoration can be recovered by ending continuous cropping. Therefore, ramie, the ideal phytoremediation material for Cd-contaminated soil, has great potential for use in the control of Cd pollution. To obtain high removal efficiencies, lots of regulators including chelating agents and plant growth regulators have been used to improve the bioavailability of metals in soil and shoot biomass, respectively.(Hasan et al. 2019 ) (Rostami &Azhdarpoor 2019 ) Plant growth regulators play a crucial role in the regulation of plant growth and development and in the response to external stresses.(Santner &Estelle 2009 ) The main plant growth regulator are auxin, gibberellin (GA), cytokinin, abscisic acid, ethylene (ETH), and Brassinolide (BR) as well as some recently identified plant regulator, including polyamines (PAs) and salicylic acid (SA). GA has been proven to enhance the resistance of plants to heavy metal stress and to promote the accumulation of heavy metals. Masood found that 10 mol L −1 GA can reverse the adverse effects of Cd on brassica.(Masood et al. 2016 ) The 10 −6 mol L −1 GA 3 treatment increased Cd accumulation by 289% and the bioaccumulation coefficient by 128% in parthenium.(Ali &Hadi 2015 ) ETH is mainly used as a ripening agent in practical applications, but several studies have demonstrated that ETH plays a vital role in Cd stress. The tolerance of drupe to Cd can be increased by maintaining an appropriate level of ETH and a low ETH sensitivity through an antioxidant defense mechanism.(Wang et al. 2020 ) SA can reduce the accumulation of Cd in the aboveground part of rice.(Wang et al. 2021a ) SA can enable plants to resist abiotic stresses, such as ultraviolet radiation, low temperatures, heat shock, water deficit, salt injury, and heavy metals, and plays a role in the cross-protection response of plants to abiotic stresses.(Madany et al. 2020 , Yadav et al. 2020 ) SA can also increase mineral nutrition in plant organs and regulate the photosynthesis system, improving overall crop quality.(Kou et al. 2021 , Sharma et al. 2020 , Yalpani &Raskin 1993 ) BR is a new plant hormone involved in plant growth and stress response and has been reported to promote plant growth, improve photosynthesis, and reduce heavy metal toxicity in plants.(Guo et al. 2018 , Li et al. 2012 ) PAs are compounds containing two or more amino groups. The raw materials used in its synthesis are ornithine and arginine. PAs play a crucial role in promoting the absorption of inorganic ions by roots, which improves resistance to stress and osmotic stress. (Pal et al. 2019 ) Binding PAs may play an essential role in resistance to Cu 2+ stress. (Zhao et al. 2008 ) Therefore, plant growth regulators can improve shoot biomass and enhance their accumulation capacity for heavy metals in aboveground plant parts. However, no effect of plant growth regulators has been observed on the enrichment and transport of heavy metals in ramie. Foliar fertilizer is a key source of nutrient elements for plant growth and development. Foliar fertilizer can also improve plants’ resistance to stress, promote plant growth and development, and increase yield. Potassium and phosphorous are the key elements for plant growth. Potassium activates many types of enzymes, which can enhance photosynthesis and the synthesis and metabolism of carbohydrates. (Zhao et al. 2020 ) The use of potassium fertilizer during production can increase the yield and stress resistance of crops. Phosphorus is a key component of nucleic acid, nucleoproteins, phospholipids, and enzymes. The use of phosphorus fertilizer during production can enhance the crops’ resistance against drought and cold.(Atafar et al. 2010 ) However, few studies have investigated the effects of fertilizers on the growth and ability of ramie to accumulate and transport Cd. The above studies have shown that GA can improve the biomass of plants and increase plant cadmium accumulation. The foliar fertilizer can provide the nutrient elements N, P, K, which are necessary for plant growth, so as to improve stress resistance of plant. However, their activity depends on the concentration of their use, the environmental factors that affect their absorption, and the physiological state of the plant(Rostami &Azhdarpoor 2019 ). It is unknown whether supplementation of nutrient elements N, P, K can better improve the biomass and enrichment capacity of Cd in ramie when GA is applied. And the effects of GA alone or in combination with KH 2 PO 4 or KNO 3 on the phytoextraction efficiency of ramie were unclear. In this study, two field experiment were conducted, one was using GA, ETH, SA, PAs, and BR foliar spray of ramie and another was using GA with KH 2 PO 4 or KNO 3 addition to Cd contaminated soils aimed to (1) investigate the treatment influence on Cd contents, translocation and accumulation in plant; and (2) estimate the treatment effects on the agronomic traits of ramie. These results will be helpful to compare the effects of different plant growth regulators and GA in combination with KH 2 PO 4 or KNO 3 as potential amendments for enhancing Cd phytoextraction by ramie. 2. Materials And Methods 2.1 Plant materials and soil sample Ramie is an asexual perennial plant propagated by using cuttings of lateral branches of approximately 15 cm in length. Ramie for Experiment A and Experiment B were arranged in two completely randomized plots with three replicates. Each plot contained six plants, planted in rows spaced 0.5 m apart, with a distance of 0.4 m between plants within rows. Experiment A: Ramie was planted in a Cd-polluted farmland in Hunan Agricultural University’s training base, Changsha City, Hunan Province, China. Experiment B: Ramie was planted in a Cd-polluted farmland in Liu yang City, Hunan Province, China. The lateral branches of ramie variety 171 were cut and propagated in June 2017. The plants were planted in the field in April 2017 and were mowed in December 2017. The ramie variety 171 was provided by the Ramie Research Institute of Hunan Agricultural University (Changsha, China). Surface soil samples (0-20 cm) were taken from the test site, Then, the soil samples were air-dried and sieved through a 2-mm nylon screen to remove any debris before testing. Six air-dried soil samples were randomly taken to determine the physical-chemical properties. (GB15618-2018) The soil type was red soil, pH= 5.73, the average Cd content in soil was 3.27 mg kg -1 , soil organic matter= 29.25 g kg -1 , total nitrogen=1.56 g kg -1 , total phosphorus= 0.51 g kg -1 , total potassium=14.71 g kg -1 。 2.2 Field experiment Experiment A and Experiment B were conducted at vigorous growing period. In experiment A, five plant growth regulators in different concentrations were sprayed into the positive and negative sides of ramie leaves in the corresponding plots. (Table 1) In experiment B, GA, KNO 3 and KH 2 PO 4 are compounded in different concentrations was sprayed onto the positive and negative sides of ramie leaves in the corresponding plots. (Table 2) This procedure was repeated five times, once every 15 days, from April 19, 2018. To reduce the effect of direct sunlight and to prevent the agents from being washed away by the rain, the agents were administered on sunny mornings when the sun is not too strong. The ramie grew in the soil for 125 days after transplantation and then harvested and divided into various parts for further processing. Before ramie was harvested, the agronomic traits of the ramie under each treatment were examined during the mature stage. A total of 10 plants were selected for measurement. A meter scale was used to measure the height of the plants. The diameter and thickness of the stems were measured using a micrometer. Ramet number was measured through manual calculation. The area of the leaves was calculated by measuring their length and width with a straightedge. The tissues were carefully washed with tap water and double-distilled water to ensure no dust or other undesirable materials remained on the surface of the samples. The tissues were dried in an oven at 60 ± 5 °C for 4 days to ensure the constant weight. The weight of the samples was then measured, and the samples were crushed into powder for the Cd analysis. Take the soil in the rhizosphere of ramie，and then the soil samples were air dried, crushed gently, and passed through a 2-mm sieve prior to the Cd analysis for calculation of index. 2.3 Determination of Cd concentration The dried plant materials and soil samples were ground into powder and sieved, and 0.5 g samples were digested in mixed acid (HNO 3 + HClO 4 [3:1, v/v]).(Tang et al. 2015a ) Cd content was determined using an atomic absorption spectrometer (SOLAAR M6). The linear fitting of the results of the samples measurements was 0.998 and the fitting degree of the equation was tested by chi square. 2.4 Statistical analysis 2.4.1 Comparison of Ramie Field Performance with different treatments Field performance among different treatments were compared using ANOVA (analysis of variance) in SAS 9.4 software (SAS Institute, Cary, NC, United States). Plant data with different treatments were considered independent variables. The mean of each trait was tested at the p < 0.05 level and p < 0.01level using Duncan’s multiple range test. (The following is the same) Evaluation of the overall field performance is a multi-criteria decision-making process that involves many factors. In this study, a Membership function (MF) value and synthetic membership function (SMF) value were used to comprehensively express overall field performance. (Jin et al. 2020 )The MF value of each field performance trait was calculated based on the following formula: $$yi \left(k\right) = \left[xi \right(k)-\text{m}\text{i}\text{n} x (k\left)\right] / \left[ \text{m}\text{a}\text{x} x \right(k) - \text{m}\text{i}\text{n} x (k\left)\right] \left(1\right)$$ Where yi (k) represents the MF value of the k th field performance trait, xi ( k ) denotes the field-recorded value of the kth field performance, and max x ( k ) and min x ( k ) represent the largest and smallest value of xi ( k ), respectively. The SMF value of each treatment was calculated based on the following formula: $$\sum _{\text{i}=1}^{\text{i}=\text{n}}yi\left(k\right)=\left[xi \right(k) - \text{m}\text{i}\text{n} x (k\left)\right] / \left[\text{m}\text{a}\text{x} x \right(k) - \text{m}\text{i}\text{n} x (k\left) \right(2)$$ 2.4.2 Comparison of cadmium related indexes of Ramie with different treatments The accumulation and absorption of cadmium in Ramie with different treatments can be shown by many indexes. (Wei et al. 2012 ) The (BCF) value of each treatment was calculated based on the following formula: $$yBCF \left(k\right)= x \text{p}\text{a}\text{r}\text{t} \text{o}\text{f} \text{p}\text{l}\text{a}\text{n}\text{t} \left(k\right) / x \text{s}\text{o}\text{i}\text{l} \left(k\right) \left(3\right)$$ Where yBCF ( k ) the Cd bioconcentration factor value (BCF) of the k th treatments, x part of plant ( k ) denotes the cadmium concentration value of the k th, and x soil ( k ) represent the cadmium concentration value of the k th soil. The Cd transfer coefficient (TF) value of each treatment was calculated based on the following formula: $$yTF \left(k\right)= x \text{a}\text{b}\text{o}\text{v}\text{e}\text{g}\text{r}\text{o}\text{u}\text{n}\text{d}\left(k\right) / x \text{u}\text{n}\text{d}\text{e}\text{r}\text{g}\text{r}\text{o}\text{u}\text{n}\text{d}\left(k\right) \left(4\right)$$ Where yTF ( k ) represents the Cd transfer coefficient value (TF) of the k th treatments, x aboveground denotes the cadmium concentration value of the k th aboveground, and x underground ( k ) represent the cadmium concentration value of the k th soil. The Enrichment quantity value of each treatment was calculated based on the following formula: $$yEQ \left(k\right)=x \text{b}\text{i}\text{o}\text{m}\text{a}\text{s}\text{s}\left(k\right) \text{*} x \text{C}\text{d} \text{c}\text{o}\text{n}\text{t}\text{e}\text{n}\text{t} (\text{a}\text{b}\text{o}\text{v}\text{e}\text{g}\text{r}\text{o}\text{u}\text{n}\text{d}+ \text{u}\text{n}\text{d}\text{e}\text{r}\text{g}\text{r}\text{o}\text{u}\text{n}\text{d}) \left(k\right) \left(5\right)$$ Where yEQ ( k ) represents the enrichment quantity value of the k th treatments, x biomass( k ) denotes the biomass of the k th treatments, and x Cd content (aboveground +underground) ( k ) represent the k th Cd content of the sum of aboveground and underground. 2.4.3 Correlation between cadmium related indexes and agronomic traits of ramie in different treatments Correlation analysis (CA analysis) was used to evaluate the relationship between the growth and development of ramie and the accumulation and absorption of Cd. Correlations between the ramie’s overall agronomic traits and the cadmium related indexes were performed using the CORR procedure in SAS 9.4 software. Pearson’s correlation coefficients and their significance were used to assess the strength of the correlations. All assays were made in triplicate. Graphs were drawn using GraphPad Prism 7.0 (GraphPad Software, San Diego, CA, USA). 3. Results 3.1. Analysis of agronomic traits and enrichment quantity after plant growth regulator treatment and mixture of GA and foliar fertilizers 3.1.1. Effects of plant growth regulators on agronomic traits and Cd enrichment The effects of plant growth regulator on the agronomic traits were evident. Plant growth varied in accordance with variety and concentration of plant growth regulators. Plant height, stem diameter, skin thickness, and leaf area were the main parameters influencing biomass. As shown in Table 3 , GA-3 and PAs-3 treatments significantly increased plant height. For all treatments, plant height significantly decreased after plants were sprayed with ETH. All hormone treatments except the ETH treatments caused significant increases in biomass accumulation compared with the control 1(CK-1) treatment. The effect of plant growth regulators treatments on the leaf area, stem diameter, and skin thickness of ramie was negligible; the ETH-3 treatment was the only treatment to significantly reduce these measures in comparison with control. Cd enrichment is the overall capacity of ramie to adsorb Cd. Treatment with PAs achieved the most noticeable effect on Cd enrichment; Cd enrichment after treatment with all three concentrations of PAs was significantly higher than after CK-1 treatment. Cd enrichment after treatment with SA-1 and SA-3 was significantly higher than that after CK-1 treatment. The BR-1 and GA-2 treatments also increased Cd enrichment. However, Cd enrichment after the ETH-1, ETH-2, ETH-3 treatments was lower than that after CK-1 treatment. 3.1.2. Effects of GA and foliar fertilizer mixture on agronomic traits and Cd enrichment The GP and GN treatments positively affected the agronomic traits and Cd enrichment of ramie (Table 4 ). Plant height and biomass of ramie under GP-2, GP-3 and GN-2, GN-3 treatments were generally significantly higher than those under CK-2, but GP-1 and GN-1 treatments were significantly lower. Among the GW treatments, Cd enrichment after the GP-3 and GN-3 treatments was significantly higher than that after CK-2 treatment. The GP-1, GP-2, GP-3 and GN-1, GN-2, GN-3 reduced the biomass and Cd enrichment of ramie more than GW-1, GW-2, GW-3. 3.2 Effects of plant growth regulators on Cd content, Cd TF, and Cd BCF of ramie 3.2.1 Effects of plant growth regulators on Cd content of ramie Compared with the control, plant growth regulator treatments significantly increased the Cd content in the aboveground ramie. The Cd content of the aboveground ramie changed in accordance with type and concentration of plant growth regulators (Figure 1.a). The BR, GA, SA, ETH, and PAs plant growth regulator treatments increased the Cd content of the aboveground ramie. According to the results, the Cd content after treatments with various concentrations of plant growth regulators, except the BR-1, GA-3, SA-2, and SA-3 treatments, was considerably higher than that of the control group. The GA-1 and SA-1 treatments exerted the strongest effect; Cd content after these treatments was 3 times higher than that after CK-1 treatment. The Cd content of the aboveground ramie in the GA-1, GA-2, GA-3 group and PAs-1, PAs-2, PAs-3 group decreased as the concentration of GA and PAs increased. The Cd content of the aboveground ramie in the ETH-1, ETH-2, ETH-3 group exhibited the opposite effect. The Cd content of the aboveground ramie in the SA-1, SA-2, SA-3 group was similar to that of the BR-1, BR-2, BR-3 group; as the concentration of SA and BR increased, the Cd content of the aboveground ramie decreased at first and then increased. The plant growth regulators affected the Cd content of both the aboveground and the underground parts of ramie. The Cd content of the underground ramie after all treatments was generally lower than that of the control group (Figure 1.b), especially the Cd content of the groups treated with BR-2 and GA-1, which was 59.45% and 54.76% lower than that of the control, respectively. Similarly, the Cd content of the groups treated with GA-3, PAs-1, and PAs-2 was significantly lower than that of the control group. The Cd content of the underground ramie treated with BR decreased when the concentration of BR increased, which was contrary to the trend for the GA and SA treatments. The Cd content of the underground ramie after the ETH and PAs treatments did not change significantly. 3.2.2 Effects of plant growth regulators on Cd TF of ramie Cd TF refers to the ratio of Cd content of the aboveground part of ramie to that of the underground part. TF is an index used to evaluate the transportation of Cd from underground to aboveground parts of plants. Figure 2 shows that the Cd TF of ramie treated with lant growth regulators significantly increased. The TFs after the ETH-1, ETH-2, ETH-3 group treatment increased with an increasing concentration of ETH. By contrast, the TFs after the GA-1, GA-2, GA-3 group and PA-1, PA-2, PA-3 group treatment decreased as the concentration of the plant growth regulators increased. The TFs after the SA-1, SA-2, SA-3 group treatment decreased at first and then increased with the concentration of SA. The GA-1 treatment was the most effective and significantly stronger than CK-1, which yielded a TF greater than 2. 3.2.3 Effects of plant growth regulators on Cd BCF of ramie A mount of heavy metals a plant absorbs and enriches from soil can be used as an indicator of the plant’s enrichment ability. The BCF of Cd is the ratio of the element content in a certain part of the plant to the corresponding element content in the soil. To a certain extent, the BCF of Cd reflects the degree of difficulty for an element to migrate through the soil–plant system and indicates Cd enrichment in plants. As shown in Figure 3.a, the Cd BCF of the aboveground ramie after the GA-1, GA-2, GA-3 group, PA-1, PA-2, PA-3 group and SA-1, SA-2, SA-3 group treatments increased with an increase in the concentration of GA, PAs and SA. The Cd BCF of the aboveground ramie after the GA-1, PAs-1, SA-1 treatments and the GA-2 treatment was significantly higher than that of the control. However, the Cd BCF of the aboveground ramie after the SA-3 treatment was significantly lower than that of the control. The Cd BCF of the aboveground ramie after the ETH-1, ETH-2, ETH-3 group treatment increased at first and then decreased, and the Cd BCF of the aboveground ramie after the ETH-2 treatment was significantly higher than that of the CK-1. The Cd BCF of the aboveground ramie after the BR-1, BR-2, BR-3 group treatment exhibited the opposite behavior to that after the ETH-1, ETH-2, ETH-3 group treatment; the Cd BCF of the aboveground ramie after the BR-2 treatment was significantly lower than that of the CK-1. The Cd BCF of the underground ramie after all plant growth regulator treatments was generally significantly lower than that after CK-1 treatment. The Cd BCF of the underground ramie after the GA-1 and SA-1, GA-3 and SA-3, PAs-2, and PAs-3 treatments were significantly lower than that after CK-1 treatment and did not markedly change after the GA-2, SA-3 and PAs-1 treatments. The changes in the Cd BCF of the underground ramie upon increasing concentrations of BR and ETH were similar to those in the aboveground Cd BCF (Figure 3.b). 3.3 Effects of GA and foliar fertilizer mixture on Cd content, Cd TF, and Cd BCF of ramie 3.3.1 Effects of GA and foliar fertilizer mixture on Cd content of ramie GA significantly increased the Cd content, TF, and BCF of the aboveground ramie (Figure 1-3). Because fertilizers composed of nitrogen and potassium are known to promote the growth and development of ramie, this study examined whether a mixture of nitrogen foliar fertilizer, potassium fertilizer, and GA could enhance the ability of ramie to absorb and enrich Cd. Figure 4.a shows that treatment with a mixture of GA mixed and foliar fertilizers did not significantly affect the Cd content of the aboveground ramie compared with CK-2 treatment, except the GN-1 treatment, which reduced the Cd content by 59.88%. The combination of GA and foliar fertilizer significantly reduced the Cd content of the aboveground ramie in comparison with GA alone. The combination of GA and foliar fertilizer did not significantly affect the Cd content of the underground ramie. The Cd content of the underground ramie after the GP-3 and GN-3 treatments was slightly higher than that after CK-2 treatment (Figure 4.b). 3.3.2 Effects of GA and foliar fertilizer mixture on Cd TF of ramie We discovered that unlike GA alone, the mixture of GA and fertilizers reduced the Cd TF of ramie. However, most compound treatments did not significantly affect the Cd TF in comparison with CK-2 treatment; only the GN-1 treatment significantly affected the Cd TF. The Cd TF after the GN-1 treatment was half that after CK-2 treatment (Figure 5 ). 3.3.3 Effects of GA and foliar fertilizer mixture on Cd BCF of ramie The Cd BCF of the aboveground ramie after the GP-1, GP-2, GP-3 group treatment and GN-1, GN-2, GN-3 group treatment were generally lower than that after the CK-2 and GW-1, GW-2, GW-3 group treatment, and the interaction between GP-1, GP-2, GP-3 group treatment and GN-1, GN-2, GN-3 group treatment made the Cd BCF of the aboveground ramie significantly lower than did the GW-1, GW-2, GW-3 group treatment with the same concentration of GA (Figure 6.a). The Cd BCF of the underground ramie after the GP-1, GP-2, GP-3 group treatment and GN-1, GN-2, GN-3 group treatment were generally higher than that after the GW-1, GW-2, GW-3 group treatment, but were not significantly different from that after CK-2 treatment, except for the GP-3 treatment (Figure 6.b). The Cd BCF of the underground ramie after the GP-2 treatment was not significantly different from that after the GW-2 treatment. Therefore, treatment with GA significantly reduces the Cd BCF of the underground part of ramie, but the mixture of GA of foliar fertilizer can negate this effect. 3.4 Correlation analysis of traits 3.4.1 Effects of plant growth regulators on correlation Figure 7 presents a significant correlation among various indicators after plant growth regulators treatment. For example, plant height was significantly correlated with leaf area and biomass. Leaf area and biomass were positively correlated with plant height, with correlation coefficients of 0.74 and 0.85 respectively. A significant positive correlation was observed between leaf area and biomass, with a correlation coefficient of 0.77. Aboveground Cd content was significantly correlated with Cd TF and aboveground Cd BCF. Cd TF and aboveground Cd BCF were positively correlated, with correlation coefficients of 0.82 and 0.84. A significant negative correlation was observed between soil Cd content, aboveground Cd BCF, and underground Cd BCF, with correlation coefficients of −0.74 and −0.79, respectively. The correlation coefficient of the significant positive correlation between Cd TF and aboveground Cd BCF was 0.73. The correlation coefficient of the significant positive correlation between aboveground Cd BCF and underground Cd BCF was 0.63. Cd enrichment had a significant positive correlation with plant height(0.72),leaf area (0.68), and biomass (0.88). 3.4.2 Effects of GA and foliar fertilizers mixture on correlation Biomass and Cd enrichments had a significantly positive correlation after treatment with GA and foliar fertilizer mixtures. (Figure 8 ) Aboveground Cd content was positively correlated with Cd TF (0.86) and aboveground Cd BCF (0.92) and negatively correlated with underground Cd BCF (−0.85) and underground Cd content (−0.71). A significant negative correlation was observed between soil Cd content and underground Cd content, with a correlation coefficient of −0.68. Cd TF was significantly and positively correlated with aboveground Cd BCF and negatively correlated with underground Cd BCF, with correlation coefficients of 0.95 and −0.86, respectively. Cd enrichment had a significant positive correlation with stem diameter (0.66), leaf area (0.71),andbiomass(0.90). 4. Discussion Treating Cd soil pollution is an urgent task, and the phytoremediation technology-based approach can achieve superior results both economically and ecologically.(Wang et al. 2021b ) Ramie is a strong candidate and can promote the green revolution. Planting ramie can not only promote the development of the textile industry but also prevent soil pollution from entering the food chain, which would positively affect human health.(Yaseen et al. 2016 ) Investigation of the physiological and molecular mechanisms of Cd in ramie is crucial to regulating the amount of Cd moving from soil to plants and repairing soil. For the short-distance transport of Cd through the roots, phytochelatin secretion and vacuolar partition via ion channels and transporters are the key elements in the absorption, transport, and accumulation of Cd; for long-distance transport, the loading and unloading of phloem is a crucial element in the transport and accumulation of Cd to ramie plants. The transport and accumulation of Cd plants also causes physiological responses to Cd stress in ramie plants, which can manifest as changes in plant growth regulators levels, photosynthesis, water absorption, and mineral element absorption.(Wang et al. 2021c , Yoneyama et al. 2015 ) This study examined the effects of several plant growth regulators and the combination of fertilizers and GA on ramie. The increases in the Cd TF of ramie after the hormone treatments may be explained by the following: plant growth regulators caused the increases in the number of physiological and biochemical molecules absorbing and carrying Cd and enhanced the Cd resistance of ramie, resulting in the Cd enrichment of the aboveground part of ramie. GA, ETH, SA, BR, and PAs play a crucial role in alleviating abiotic stress and regulating the growth and development of plants.(Alcazar et al. 2020 , Bajguz 2011 ) According to results of this study, SA, BR, and PA played an essential role in the transportation of Cd in both the aboveground and underground parts of ramie, and the effects of BR and PA were dependent on concentration and ramie part. GA is a plant growth stimulation hormone that regulates several physiological and biochemical processes, promotes growth and development, affects morphogenesis, and plays an essential role in the response to both biotic and abiotic stresses in plants.(Shu et al. 2018 , Spence &Bais 2015 ) Studies have demonstrated that plant growth regulators such as SA, GA, and indole-3-acetic acid (IAA) can alleviate abiotic stresses.(Hussain et al. 2020 , Jia et al. 2021 , Saleem et al. 2015 , Zhang et al. 2015 ) For example, the use of GA on leaves can reduce the uptake of nickel by mung bean plants, increase biomass, and promote growth. The importance of GA under abiotic stress has been well documented. This study confirmed that GA can promote the growth of ramie and improve its ability to absorb and enrich Cd. ETH can inhibit the growth of plants, and the results in subsection 3.1 indicated that ETH caused a decrease in biomass and Cd enrichment. Studies on other plants have demonstrated that ETH can promote leaf abscission and plant maturation and inhibit apical dominance but that spraying at certain stages may produce the opposite effects.(Schubert et al. 2019 ) The height of ramie decreased after ETH was applied, but the tillering and leaf area of ramie did not change significantly. ETH reduces the main components of biomass, thereby leading to a decrease of biomass. The enrichment of Cd in the plants decreased in accordance with changes in biomass. N, P, and K are vital nutrients for plant growth. The addition of fertilizer to the cultivation process benefits the growth and development of crops. (Xia et al. 2020 ) The results of this study demonstrated that GA alone or in combination with KNO 3 or KH 2 PO 4 positively influenced the agronomic traits of ramie, indicating that the mixture of GA and fertilizer with N or P exhibited the same effects on the growth and development of ramie as in previous studies. In addition, treatment with GP (including GP-1, GP-2, GP-3) and GN (including GN-1, GN-2, GN-3) promoted the enrichment of Cd in ramie. This may be related to the physiological process ramie undergoes during heavy metal stress. A key regulator of plant growth and development, the functional site of GA at the cellular, tissue, and organ level of ramie is unknown.(Ubeda-Tomas et al. 2008 ) The site of application for GA on ramie can be a subject for future research. P +, K +, and Cd+ share the same transport pathway in plants. Although treatment with fertilizer promoted the growth and development of the plants, their Cd+ absorption and transport capacity decreased. Phosphates can increase the ionic strength of Cd adsorption.(Yan et al. 2015 ) Therefore, the decrease caused by GN (including GN-1, GN-2, GN-3)was more apparent than that caused by GP, especially in aboveground Cd content and aboveground BCF. The BCF after the GP (including GP-1, GP-2, GP-3) and GN (including GN-1, GN-2, GN-3) treatments was higher than that after the GW-1, GW-2, GW-3 group treatment, which may be related to the chelation of root exudates. Plants’ response to Cd stress is a complex physiological process involving ion transporters.(Lu et al. 2020 ) Studies on yeast(Mesquita et al. 2016 ), Arabidopsis(Zheng et al. 2018 ), and rice(Pan et al. 2021 ) have demonstrated that adenosine triphosphate–binding cassette transporters, heavy metal–associated transporters, and natural resistance–associated macrophage protein transporters are involved in the response to Cd stress. The results of the study revealed common phenomena among the hormone treatments: the aboveground Cd content and Cd BCF increased; the underground Cd content and Cd BCF decreased; and the TF increased significantly after all hormone treatments. Traditionally a textile crop, ramie has high cellulose, hemicellulose, and lignin content in the phloem, which establishes the conditions for Cd accumulation. The pulp inside ramie contains a large number of vessels that can transport Cd + . Hormones promote the growth of the aboveground part of ramie, the transport of Cd, and the accumulation of Cd in the underground part of the plant. Liu (Liu et al. 2021 ) discovered that high concentrations of endogenous ETH delayed the formation of an ectoblast barrier and promoted the accumulation of Cd in the root ectoblasts. Studies by Neumann(Neumann 2015 ) have demonstrated that ETH-mediated responses usually have high genotypic variability and may partially share common pathways under certain nutritional constraints. Although the biomass and Cd enrichment of ramie decreased after the ETH treatment, the Cd content and BCF of the aboveground ramie increased under high TFs. 5. Conclusions Plant growth regulators and foliar fertilizers increased the Cd content of the aboveground ramie, reduced the Cd content of the underground ramie, and increased the TF. Among them, GA-1 increased the Cd content of the aboveground ramie to 3 times more than that of the control and reduced the Cd content of the underground ramie by 54.76%. Salicylic acid (SA) increased the Cd content of the aboveground ramie to 3 times more than that of the control. The combination of GA and foliar fertilizer reduced the Cd content of the aboveground and underground ramie and the TF and BCF of the underground ramie. After the hormones were sprayed, the TF of ramie had a significant positive correlation with the Cd content of the aboveground ramie; the BCF of the aboveground ramie had a significant positive correlation with the Cd content and TF of the aboveground ramie. In actual production, the proper concentration of PAs, GA, SA and BR can be sprayed during the prosperous period of ramie, which will increase the biomass of ramie and increase the efficiency of cadmium enrichment of ramie. It is generally not recommended to mix plant regulators and foliar fertilizers. Declarations Availability of data and materials: All data generated or analyzed during this study are included in this published article. Funding : This research was funded by Key R & D Program of Hunan Province, grant number 2022NK2017. Author information Affiliation s Ramie Research Institute (Hunan Agricultural University), Changsha, 410128, China Key Laboratory of germplasm resources innovation and utilization, Changsha, 410128, China Wenxian Peng, Yejun He, Si He, Jinfeng Luo, Yi Zeng, Xiaoyang Zhang, Yingyi Huo, Yucheng Jie, Hucheng Xing Authors Contributions : Conceptualization, Wenxian.Peng. and Yejun.He.; methodology, Hucheng. Xing.; software, Wenxian. Peng. and Yejun.He.; validation, Wenxian.Peng.; investigation, Xiaoyang. Zhang.; resources, Yucheng. Jie.; data curation, Jinfeng. Luo. and Yi. Zeng.; writing-original draft preparation, Wenxian.Peng.; writing-review and editing, Wenxian.Peng., Si.He. and Yingyi.Huo. ; visualization, Wenxian.Peng.; supervision, Hucheng. Xing.; project administration, Hucheng. Xing.; funding acquisition, Hucheng. Xing. All authors have read and agreed to the published version of the manuscript. Corresponding author Correspondence to Hucheng Xing Ethics declarations Ethics approval : The manuscripts reporting studies are not applicable for human participants, human data, or human tissue. The manuscript does not contain any individual person’s data in any form. Consent to participate : Not applicable. Consent to Publish ：All authors agree to publish. 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Experiment Treatments concentration A CK-1 0 mg L −1 GA-1 50mg L −1 GA-2 100mg L −1 GA-3 200mg L −1 ETH-1 50mg L −1 ETH-2 100mg L −1 ETH-3 200mg L −1 SA-1 50mg L −1 SA-2 100mg L −1 SA-3 200mg L −1 PAs-1 0.1mmol PAs-2 1mmol PAs-3 10mmol BR-1 0.1mg L −1 BR-2 1mg L −1 BR-3 10mg L −1 Note: GA represents gibberellin, ETH represents ethylene, SA represents salicylic acid, PAs represents polyamine, BR represents Brassinolide, the number 1,2 and 3 indicates the increase of different treatment concentrations, respectively. Table 2. Concentrations of different plant growth regulator and fertilizers. Experiment Treatments concentration B CK-2 0 mg L −1 +0% GW-1 50mg L −1 +0% GW-2 100mg L −1 +0% GW-3 200mg L −1 +0% GP-1 50mg L −1 +0.2% GP-2 100mg L −1 +0.4% GP-3 200mg L −1 +0.6% GN-1 50mg L −1 +1% GN-2 100mg L −1 +1.5% GN-3 200mg L −1 +2% Note: GW: GA+ Water; GP: GA+ KH 2 PO 4 ; GN: GA+KNO 3 ; The number 1,2 and 3 indicates the increase of different treatment concentrations, respectively. Table 3 Agronomic traits and Cd enrichment of ramie under different hormones Treatments Plant height (cm) Stem diameter (mm) Skin thickness (mm) Leaf area (cm 2 ) Biomass (kg ha −1 ) Cd enrichment (mg ha −1 ) CK-1 212.07±5.6DE 11.45±0.58A 0.68±0.01AB 228.93±4.94AB 1659.24±19.09I 22709.46±70.71I BR-1 215.03±2.87DE 11.02±1.26A 0.68±0.06AB 230.73±1.37AB 2379.69±72.11E 43048.59±173.46E BR-2 217.30±5.28CDE 10.87±0.70A 0.71±0.10AB 222.52±5.11AB 2378.18±79.00E 23480.56±74.83E BR-3 221.70±5.86BCDE 11.64±0.91A 0.74±0.06AB 220.67±13.82B 2339.15±43.59EF 34640.09±71.18EF ETH-1 145.30±3.82F 12.94±0.64A 0.68±0.04AB 183.24±8.37C 1025.15±8.89J 15824.90±142.77J ETH-2 132.05±2.60G 12.39±1.32A 0.75±0.12AB 159.02±5.07D 965.19±25.98J 14297.68±72.57J ETH-3 132.41±3.58G 11.90±1.06A 0.72±0.08AB 128.65±3.93E 1060.80±88.88J 17588.06±367.99J GA-1 212.00±3.00DE 12.10±0.69A 0.73±0.11AB 161.74±3.78D 1859.60±46.16H 28856.40±132.63H GA-2 228.00±4.35BC 12.02±0.75A 0.84±0.12A 195.03±7.10C 2019.60±20.52G 38068.73±500.58G GA-3 249.33±4.62A 12.80±0.60A 0.67±0.04AB 200.62±9.68C 2645.83±109.34D 32349.68±146.97D PAs-1 219.73±1.99BCDE 11.82±1.19A 0.69±0.06AB 224.50±7.87AB 2568.07±90.14D 40748.15±297.18D PAs-2 223.27±1.16BCD 12.35±1.26A 0.70±0.02AB 218.02±5.06B 3304.81±86.69B 49685.24±362.86B PAs-3 241.87±0.29A 11.67±1.17A 0.83±0.19A 227.30±12.26AB 3980.73±22.68A 58875.00±711.80A SA-1 209.80±2.33E 11.43±1.11A 0.69±0.07AB 220.34±3.94B 2209.42±69.28F 36772.11±432.05F SA-2 220.27±0.93BCDE 11.83±1.50A 0.58±0.09B 196.79±6.54C 2254.69±20.00EF 21772.79±285.77EF SA-3 230.03±13.83B 11.91±1.71A 0.80±0.17AB 236.02±5.82A 2827.55±54.44C 35542.30±216.07FG Treatment F P F P F P F P F P F P 164. 90 *** 0.82 0.65 1.35 0.23 56.18 *** 515.02 *** 3284.93 *** Note: The same letters within a column indicate no significant differences (P > 0.01) among the treatments and CK-1. Values are means ± SD (n = 3). ***, P < 0.001; **, P <0.01; *, P < 0.05., Duncan’s multiple range test. Table 4 Agronomic traits and Cd enrichment of ramie under different hormones and fertilizers Treatments Plant height (cm) Stem diameter (mm) Skin thickness (mm) Leaf area (cm 2 ) Biomass (kg ha −1 ) Cd enrichment (mg ha −1 ) CK-2 183.8±4.44D 10.25±0.40AB 0.62±0.03A 236.04±3.72D 752.62±12.30G 6894.00±149.52E GP-1 171.2±1.97E 8.73±0.67B 0.59±0.07A 201.55±5.73E 1005.32±6.65F 7144.47±100.33E GP-2 219.3±3.52B 11.48±0.59A 0.74±0.10A 258.05±4.41C 1336.27±6.81D 11505.28±818.62C GP-3 225.57±4.55AB 12.28±0.48A 0.66±0.09A 272.58±3.84AB 1449.54±49.46C 15198.43±694.29B GN-1 182.68±3.83D 10.38±0.44AB 0.68±0.10A 224.24±6.61D 1153.65±45.72E 8540.86±224.84D GN-2 226.20±5.00AB 12.37±1.49A 0.73±0.01A 265.71±5.69BC 1398.06±4.06CD 10308.36±617.67C GN-3 231.43±3.19A 11.92±0.39A 0.75±0.09A 281.76±7.61A 1456.82±42.55C 15689.95±34.72B GW-1 206.67±4.38C 12.01±0.53A 0.7±0.09A 275.8±6.57AB 1629.20±81.64B 15444.82±867.03B GW-2 189.03±6.83D 11.92±1.47A 0.7±0.04A 256.23±3.79C 2325.11±49.97A 19608.43±48.11A GW-3 218.00±2.62B 10.72±0.68AB 0.56±0.08A 275.13±7.71AB 1626.20±31.61B 14240.09±876.31B Treatment F P F P F P F P F P F P 78.27 *** 6.12 *** 2.26 0.06 61.76 *** 318.68 *** 153.03 *** Note: The same letters within a column indicate no significant differences (P > 0.01) among the treatments and CK. Values are means ± SD (n = 3). ***, P < 0.001; **, P <0.01; *, P < 0.05., Duncan’s multiple range test. Supplementary Files GraphicalAbstract.docx Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-1197456","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":85098614,"identity":"5588620e-4143-44f3-a06d-8b9907798a7d","order_by":0,"name":"Wenxian peng","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAAAzElEQVRIiWNgGAWjYBACNvaGxMc/ftjIsbE3HyBOCx/PgcfGjD1pxnw8xxKI0yIn4fhMmoHtcOI8CR8DIh0mwZwgXcCTZswmwfPxxhsGOzndBkJapNsSjGdYAP0i3bvZcg5DsrHZAUJaZM4kJPCAbJE5u02ah+FA4jaCWiTyPxzgAfqlTSLnGbFaEhKboVrYiNTCcyCZcSYwkNl4jhlbzjEgwi/y7Q3pPz4Ao1K+vfnhjTcVdnIEtaAACR4iowZZC6k6RsEoGAWjYEQAABNtQAWOsxPfAAAAAElFTkSuQmCC","orcid":"https://orcid.org/0000-0002-4903-7045","institution":"Hunan Agricultural University","correspondingAuthor":true,"submittingAuthor":false,"prefix":"","firstName":"Wenxian","middleName":"","lastName":"peng","suffix":""},{"id":85098615,"identity":"9372c73a-b448-4a3a-8ec7-bfe636798dc8","order_by":1,"name":"Yejun He","email":"","orcid":"","institution":"Hunan Agricultural University","correspondingAuthor":false,"submittingAuthor":false,"prefix":"","firstName":"Yejun","middleName":"","lastName":"He","suffix":""},{"id":85098616,"identity":"a75affb1-3791-4955-bd37-ed1e07938bc4","order_by":2,"name":"Si He","email":"","orcid":"","institution":"Hunan Agricultural University","correspondingAuthor":false,"submittingAuthor":false,"prefix":"","firstName":"Si","middleName":"","lastName":"He","suffix":""},{"id":85098617,"identity":"c56c8f14-ba07-46cd-ac56-6243449ff2a6","order_by":3,"name":"Jingfeng Luo","email":"","orcid":"","institution":"Hunan Agricultural University","correspondingAuthor":false,"submittingAuthor":false,"prefix":"","firstName":"Jingfeng","middleName":"","lastName":"Luo","suffix":""},{"id":85098618,"identity":"2c3bbf1f-75cc-4b94-9fc8-9ccc1f0bbaff","order_by":4,"name":"Yi Zeng","email":"","orcid":"","institution":"Hunan Agricultural University","correspondingAuthor":false,"submittingAuthor":false,"prefix":"","firstName":"Yi","middleName":"","lastName":"Zeng","suffix":""},{"id":85098619,"identity":"3a6cc7c9-1d10-4287-9ab5-498d159eeff9","order_by":5,"name":"Xiaoyang Zhang","email":"","orcid":"","institution":"Hunan Agricultural University","correspondingAuthor":false,"submittingAuthor":false,"prefix":"","firstName":"Xiaoyang","middleName":"","lastName":"Zhang","suffix":""},{"id":85098620,"identity":"d9e06e63-02b1-4ae4-86e6-21cf7cecf997","order_by":6,"name":"Yingyi Huo","email":"","orcid":"","institution":"Hunan Agricultural University","correspondingAuthor":false,"submittingAuthor":false,"prefix":"","firstName":"Yingyi","middleName":"","lastName":"Huo","suffix":""},{"id":85098621,"identity":"3f0de928-b11c-487c-a6c5-2282de54d03f","order_by":7,"name":"Yucheng Jie","email":"","orcid":"","institution":"Hunan Agricultural University","correspondingAuthor":false,"submittingAuthor":false,"prefix":"","firstName":"Yucheng","middleName":"","lastName":"Jie","suffix":""},{"id":85098622,"identity":"85810470-fc9d-424a-845e-a6702bfa25af","order_by":8,"name":"Hucheng Xing","email":"","orcid":"","institution":"Hunan Agricultural University","correspondingAuthor":false,"submittingAuthor":false,"prefix":"","firstName":"Hucheng","middleName":"","lastName":"Xing","suffix":""}],"badges":[],"createdAt":"2021-12-23 01:09:27","currentVersionCode":1,"declarations":"","doi":"10.21203/rs.3.rs-1197456/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-1197456/v1","draftVersion":[],"editorialEvents":[],"editorialNote":"","failedWorkflow":false,"files":[{"id":18614620,"identity":"0dcb8963-899c-4431-a70d-5d12207278a2","added_by":"auto","created_at":"2022-02-25 14:49:14","extension":"png","order_by":1,"title":"Figure 1","display":"","copyAsset":false,"role":"figure","size":165616,"visible":true,"origin":"","legend":"\u003cp\u003e(a) Cd content in aboveground ramie sprayed by plant growth regulators; (b)Cd content in underground ramie sprayed by plant growth regulators; Bars marked with different letters are significantly different among treatments (P \u0026lt; 0.01). Values are means ± SD (n = 3). Duncan’s multiple range test.\u003c/p\u003e","description":"","filename":"fig1.png","url":"https://assets-eu.researchsquare.com/files/rs-1197456/v1/43ad43a2e16e6fc3c17ee4a4.png"},{"id":18614403,"identity":"4568816c-93fe-4f22-861b-735fb95ba61a","added_by":"auto","created_at":"2022-02-25 14:46:14","extension":"png","order_by":2,"title":"Figure 2","display":"","copyAsset":false,"role":"figure","size":90448,"visible":true,"origin":"","legend":"\u003cp\u003eCd translocation factors in aboveground ramie sprayed by plant growth regulators; Bars marked with different letters are significantly different among treatments (P \u0026lt; 0.01). Values are means ± SD (n = 3). Duncan’s multiple range test.\u003c/p\u003e","description":"","filename":"fig2.png","url":"https://assets-eu.researchsquare.com/files/rs-1197456/v1/c73e65263964b7bdc208d47a.png"},{"id":18614404,"identity":"0cd60ef4-cdd3-46a5-b80f-0153c228b597","added_by":"auto","created_at":"2022-02-25 14:46:14","extension":"png","order_by":3,"title":"Figure 3","display":"","copyAsset":false,"role":"figure","size":197160,"visible":true,"origin":"","legend":"\u003cp\u003e(a) Cd bioconcentration factors in aboveground ramie sprayed by plant growth regulators; (b)Cd bioconcentration factors in underground ramie sprayed by plant growth regulators; Bars marked with different letters are significantly different among treatments (P \u0026lt; 0.01). Values are means ± SD (n = 3). Duncan’s multiple range test.\u003c/p\u003e","description":"","filename":"fig3.png","url":"https://assets-eu.researchsquare.com/files/rs-1197456/v1/c6470866aa71df0aaa27aece.png"},{"id":18614621,"identity":"853e866f-6969-4ca1-93a4-09c2fab879a3","added_by":"auto","created_at":"2022-02-25 14:49:14","extension":"png","order_by":4,"title":"Figure 4","display":"","copyAsset":false,"role":"figure","size":106779,"visible":true,"origin":"","legend":"\u003cp\u003e(a) Cd content in aboveground ramie by spraying mixed plant growth regulator and fertilizers;(b). Cd content in underground ramie by spraying mixed plant growth regulators and fertilizers; Bars marked with different letters are significantly different among treatments (P \u0026lt; 0.01). Values are means ± SD (n = 3). Duncan’s multiple range test.\u003c/p\u003e","description":"","filename":"fig4.png","url":"https://assets-eu.researchsquare.com/files/rs-1197456/v1/d570aec79af2e2f3eef22d09.png"},{"id":18614772,"identity":"fd79c947-45e1-4523-bb96-9fffe6d3b70c","added_by":"auto","created_at":"2022-02-25 14:52:14","extension":"png","order_by":5,"title":"Figure 5","display":"","copyAsset":false,"role":"figure","size":66761,"visible":true,"origin":"","legend":"\u003cp\u003eTranslocation factors (TFs) from underground to aboveground treated by mixed plant growth regulator and fertilizers; Bars marked with different letters are significantly different among treatments (P \u0026lt; 0.01). Values are means ± SD (n = 3). Duncan’s multiple range test.\u003c/p\u003e","description":"","filename":"fig5.png","url":"https://assets-eu.researchsquare.com/files/rs-1197456/v1/01ade2ef6cba0e44ba362a80.png"},{"id":18614409,"identity":"0ef78dcf-4675-4c38-9ee6-49a290dc9392","added_by":"auto","created_at":"2022-02-25 14:46:14","extension":"png","order_by":6,"title":"Figure 6","display":"","copyAsset":false,"role":"figure","size":100124,"visible":true,"origin":"","legend":"\u003cp\u003e(a) Cd bioconcentration factors in aboveground ramie by mixed plant growth regulator and fertilizers;(b). Cd bioconcentration factors in underground ramie by spraying mixed plant growth regulator and fertilizers; Bars marked with different letters are significantly different among treatments (P \u0026lt; 0.01). Values are means ± SD (n = 3). Duncan’s multiple range test.\u003c/p\u003e\u003cp\u003e\u003cbr\u003e\u003c/p\u003e","description":"","filename":"fig6.png","url":"https://assets-eu.researchsquare.com/files/rs-1197456/v1/614e823b1e7140d1965c1637.png"},{"id":18614411,"identity":"1676d658-ae56-4195-9728-bc9c3d2a4010","added_by":"auto","created_at":"2022-02-25 14:46:14","extension":"png","order_by":7,"title":"Figure 7","display":"","copyAsset":false,"role":"figure","size":80419,"visible":true,"origin":"","legend":"\u003cp\u003e\tCorrelation index of relevant indicators by plant growth regulators treatments. The shades of the colors and the corresponding numbers represent correlations. The darker the blue, the greater the negative correlation, and the darker the gray, the greater the positive correlation. Pearson’s correlation coefficients were used.\u003c/p\u003e","description":"","filename":"fig7.png","url":"https://assets-eu.researchsquare.com/files/rs-1197456/v1/d21e09fd7b5dd6daa54af4bf.png"},{"id":18614406,"identity":"011d1f43-f5fb-4a42-a8c7-88e8ee40845e","added_by":"auto","created_at":"2022-02-25 14:46:14","extension":"png","order_by":8,"title":"Figure 8","display":"","copyAsset":false,"role":"figure","size":71995,"visible":true,"origin":"","legend":"\u003cp\u003eCorrelation index of relevant indicators by mixed plant growth regulator and fertilizers. The shades of the colors and the corresponding numbers represent correlations. The darker the blue, the greater the negative correlation, and the darker the gray, the greater the positive correlation. Pearson’s correlation coefficients were used.\u003c/p\u003e","description":"","filename":"fig8.png","url":"https://assets-eu.researchsquare.com/files/rs-1197456/v1/3dc5eba3218822f1e80fbfa6.png"},{"id":19897531,"identity":"c35dd5e6-7328-4862-96d3-6aab2a4f35d6","added_by":"auto","created_at":"2022-04-02 19:53:54","extension":"pdf","order_by":0,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":1289195,"visible":true,"origin":"","legend":"","description":"","filename":"manuscript.pdf","url":"https://assets-eu.researchsquare.com/files/rs-1197456/v1/fdfbb95c-a360-4995-a81c-f46e4166fc9f.pdf"},{"id":18614405,"identity":"217a9030-d89b-467a-9b4a-5db2b5ecd145","added_by":"auto","created_at":"2022-02-25 14:46:14","extension":"docx","order_by":1,"title":"","display":"","copyAsset":false,"role":"supplement","size":11846,"visible":true,"origin":"","legend":"","description":"","filename":"GraphicalAbstract.docx","url":"https://assets-eu.researchsquare.com/files/rs-1197456/v1/2b54d9ecd45b146a910a92d8.docx"}],"financialInterests":"","formattedTitle":"\u003cp\u003eExogenous plant growth regulator and foliar fertilizers for phytoextraction of cadmium with \u003cem\u003eBoehmeria nivea \u003c/em\u003e[L.] Gaudich from contaminated field soil.\u003c/p\u003e","fulltext":[{"header":"1. Introduction","content":"\u003cp\u003eWith the advances in urbanization and industrialization, heavy metal pollution in China has become a serious problem. (Cai et al. \u003cspan class=\"CitationRef\"\u003e2019\u003c/span\u003e, Huang et al. \u003cspan class=\"CitationRef\"\u003e2017b\u003c/span\u003e, Zeng et al. \u003cspan class=\"CitationRef\"\u003e2017\u003c/span\u003e) According to a 2014 survey of soil pollution in China, 16.1% of the country\u0026rsquo;s soil contamination exceeded the legal limit, with heavy metal pollution contributing the most (82%). Cadmium (Cd) is a nonbiological essential heavy metal and one of the most toxic components of heavy metal pollution.(Koleli et al. \u003cspan class=\"CitationRef\"\u003e2004\u003c/span\u003e) Approximately 20% of total cultivated lands were contaminated with Cd in China.(Xue et al. \u003cspan class=\"CitationRef\"\u003e2014\u003c/span\u003e) Because of its similar chemical structure to zinc, Cadmium is easily absorbed by plants and can cause serious toxicity to plants, animals and humans even at low concentrations (Malandrino et al. \u003cspan class=\"CitationRef\"\u003e2011\u003c/span\u003e) Cadmium in soil can reduce plant growth, biomass, crop yield, and quality in plant(Ramzani et al. \u003cspan class=\"CitationRef\"\u003e2016\u003c/span\u003e) The ecotoxicity of Cadmium is relatively large, and the impact on the environment and the population health is more worthy of attention and the increasing population and decreasing farmland area seriously threaten the security of food, fiber, and so on. Hence, remediate and reuse the Cd-polluted lands is becoming more and more necessary and urgent. And there is a critical need to develop efficient techniques to remediate soil contaminated with Cd.\u003c/p\u003e\n\u003cp\u003ePhytoremediation is a method of remediation that considers both ecological and economic effects and is a green technology developed for its strong potential to remove environmental pollution. (Huang et al. \u003cspan class=\"CitationRef\"\u003e2017a\u003c/span\u003e, Yang \u0026amp;Shen \u003cspan class=\"CitationRef\"\u003e2020\u003c/span\u003e, Zhu et al. \u003cspan class=\"CitationRef\"\u003e2020\u003c/span\u003e) It is often used in large-scale decontamination projects and has attracted considerable attention in recent years(Liang et al. \u003cspan class=\"CitationRef\"\u003e2021\u003c/span\u003e) Phytoremediation can mainly be categorized into phytostabilization, photoevaporation, and phytoextraction according to the uptake mechanisms. Phytoextraction is considered more effective because it can permanently remove metals from contaminated sites. The repair ability of this plant depends not only on its biomass, but also on its absorptive capacity(Zhao et al. \u003cspan class=\"CitationRef\"\u003e2021\u003c/span\u003e). Ramie (\u003cem\u003eBoehmeria nivea\u003c/em\u003e [L.] Gaudich.) is a highly adaptable herbaceous perennial root that has been cultivated for more than 2000 years in China.(Tang et al. \u003cspan class=\"CitationRef\"\u003e2015b\u003c/span\u003e) Its fast growth, high fecundity, and high biological yield(Ali \u0026amp;Hadi \u003cspan class=\"CitationRef\"\u003e2015\u003c/span\u003e) make up for the deficiencies of other hyperaccumulators, such as Sedum alfredii (Tao et al. \u003cspan class=\"CitationRef\"\u003e2020\u003c/span\u003e) and sunflowers(Zamani et al. \u003cspan class=\"CitationRef\"\u003e2020\u003c/span\u003e). Research has demonstrated that ramie can remove a fair amount of metal from the soils due to its stronger root system, faster growth rate, and higher biomass(Yang et al. \u003cspan class=\"CitationRef\"\u003e2010\u003c/span\u003e). Ramie is commonly used for its fiber, its products do not enter the food chain, and it is not associated with any health risk. Researchers have also modified varieties of ramie at the genetic level to improve its tolerance and ability to accumulate heavy metals.(Zhu et al. \u003cspan class=\"CitationRef\"\u003e2020\u003c/span\u003e) Moreover, ramie is a permanent crop that provides ecological and economic benefits to cultivation measures, and the cost of restoration can be recovered by ending continuous cropping. Therefore, ramie, the ideal phytoremediation material for Cd-contaminated soil, has great potential for use in the control of Cd pollution.\u003c/p\u003e\n\u003cp\u003eTo obtain high removal efficiencies, lots of regulators including chelating agents and plant growth regulators have been used to improve the bioavailability of metals in soil and shoot biomass, respectively.(Hasan et al. \u003cspan class=\"CitationRef\"\u003e2019\u003c/span\u003e) (Rostami \u0026amp;Azhdarpoor \u003cspan class=\"CitationRef\"\u003e2019\u003c/span\u003e) Plant growth regulators play a crucial role in the regulation of plant growth and development and in the response to external stresses.(Santner \u0026amp;Estelle \u003cspan class=\"CitationRef\"\u003e2009\u003c/span\u003e) The main plant growth regulator are auxin, gibberellin (GA), cytokinin, abscisic acid, ethylene (ETH), and Brassinolide (BR) as well as some recently identified plant regulator, including polyamines (PAs) and salicylic acid (SA). GA has been proven to enhance the resistance of plants to heavy metal stress and to promote the accumulation of heavy metals. Masood found that 10 mol L\u003csup\u003e\u0026minus;1\u003c/sup\u003e GA can reverse the adverse effects of Cd on brassica.(Masood et al. \u003cspan class=\"CitationRef\"\u003e2016\u003c/span\u003e) The 10\u003csup\u003e\u0026minus;6\u003c/sup\u003e mol L\u003csup\u003e\u0026minus;1\u003c/sup\u003e GA\u003csub\u003e3\u003c/sub\u003e treatment increased Cd accumulation by 289% and the bioaccumulation coefficient by 128% in parthenium.(Ali \u0026amp;Hadi \u003cspan class=\"CitationRef\"\u003e2015\u003c/span\u003e) ETH is mainly used as a ripening agent in practical applications, but several studies have demonstrated that ETH plays a vital role in Cd stress. The tolerance of drupe to Cd can be increased by maintaining an appropriate level of ETH and a low ETH sensitivity through an antioxidant defense mechanism.(Wang et al. \u003cspan class=\"CitationRef\"\u003e2020\u003c/span\u003e) SA can reduce the accumulation of Cd in the aboveground part of rice.(Wang et al. \u003cspan class=\"CitationRef\"\u003e2021a\u003c/span\u003e) SA can enable plants to resist abiotic stresses, such as ultraviolet radiation, low temperatures, heat shock, water deficit, salt injury, and heavy metals, and plays a role in the cross-protection response of plants to abiotic stresses.(Madany et al. \u003cspan class=\"CitationRef\"\u003e2020\u003c/span\u003e, Yadav et al. \u003cspan class=\"CitationRef\"\u003e2020\u003c/span\u003e) SA can also increase mineral nutrition in plant organs and regulate the photosynthesis system, improving overall crop quality.(Kou et al. \u003cspan class=\"CitationRef\"\u003e2021\u003c/span\u003e, Sharma et al. \u003cspan class=\"CitationRef\"\u003e2020\u003c/span\u003e, Yalpani \u0026amp;Raskin \u003cspan class=\"CitationRef\"\u003e1993\u003c/span\u003e) BR is a new plant hormone involved in plant growth and stress response and has been reported to promote plant growth, improve photosynthesis, and reduce heavy metal toxicity in plants.(Guo et al. \u003cspan class=\"CitationRef\"\u003e2018\u003c/span\u003e, Li et al. \u003cspan class=\"CitationRef\"\u003e2012\u003c/span\u003e) PAs are compounds containing two or more amino groups. The raw materials used in its synthesis are ornithine and arginine. PAs play a crucial role in promoting the absorption of inorganic ions by roots, which improves resistance to stress and osmotic stress. (Pal et al. \u003cspan class=\"CitationRef\"\u003e2019\u003c/span\u003e) Binding PAs may play an essential role in resistance to Cu\u003csup\u003e2+\u003c/sup\u003e stress. (Zhao et al. \u003cspan class=\"CitationRef\"\u003e2008\u003c/span\u003e) Therefore, plant growth regulators can improve shoot biomass and enhance their accumulation capacity for heavy metals in aboveground plant parts. However, no effect of plant growth regulators has been observed on the enrichment and transport of heavy metals in ramie.\u003c/p\u003e\n\u003cp\u003eFoliar fertilizer is a key source of nutrient elements for plant growth and development. Foliar fertilizer can also improve plants\u0026rsquo; resistance to stress, promote plant growth and development, and increase yield. Potassium and phosphorous are the key elements for plant growth. Potassium activates many types of enzymes, which can enhance photosynthesis and the synthesis and metabolism of carbohydrates. (Zhao et al. \u003cspan class=\"CitationRef\"\u003e2020\u003c/span\u003e) The use of potassium fertilizer during production can increase the yield and stress resistance of crops. Phosphorus is a key component of nucleic acid, nucleoproteins, phospholipids, and enzymes. The use of phosphorus fertilizer during production can enhance the crops\u0026rsquo; resistance against drought and cold.(Atafar et al. \u003cspan class=\"CitationRef\"\u003e2010\u003c/span\u003e) However, few studies have investigated the effects of fertilizers on the growth and ability of ramie to accumulate and transport Cd.\u003c/p\u003e\n\u003cp\u003eThe above studies have shown that GA can improve the biomass of plants and increase plant cadmium accumulation. The foliar fertilizer can provide the nutrient elements N, P, K, which are necessary for plant growth, so as to improve stress resistance of plant. However, their activity depends on the concentration of their use, the environmental factors that affect their absorption, and the physiological state of the plant(Rostami \u0026amp;Azhdarpoor \u003cspan class=\"CitationRef\"\u003e2019\u003c/span\u003e). It is unknown whether supplementation of nutrient elements N, P, K can better improve the biomass and enrichment capacity of Cd in ramie when GA is applied. And the effects of GA alone or in combination with KH\u003csub\u003e2\u003c/sub\u003ePO\u003csub\u003e4\u003c/sub\u003e or KNO\u003csub\u003e3\u003c/sub\u003e on the phytoextraction efficiency of ramie were unclear.\u003c/p\u003e\n\u003cp\u003eIn this study, two field experiment were conducted, one was using GA, ETH, SA, PAs, and BR foliar spray of ramie and another was using GA with KH\u003csub\u003e2\u003c/sub\u003ePO\u003csub\u003e4\u003c/sub\u003e or KNO\u003csub\u003e3\u003c/sub\u003e addition to Cd contaminated soils aimed to (1) investigate the treatment influence on Cd contents, translocation and accumulation in plant; and (2) estimate the treatment effects on the agronomic traits of ramie. These results will be helpful to compare the effects of different plant growth regulators and GA in combination with KH\u003csub\u003e2\u003c/sub\u003ePO\u003csub\u003e4\u003c/sub\u003e or KNO\u003csub\u003e3\u003c/sub\u003e as potential amendments for enhancing Cd phytoextraction by ramie.\u003c/p\u003e"},{"header":"2. Materials And Methods","content":"\u003cp\u003e\u003cstrong\u003e2.1 Plant materials and soil sample\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eRamie is an asexual perennial plant propagated by using cuttings of lateral branches of approximately 15 cm in length. Ramie for Experiment A and Experiment B were arranged in two completely randomized plots with three replicates. Each plot contained six plants, planted in rows spaced 0.5 m apart, with a distance of 0.4 m between plants within rows. Experiment A: Ramie was planted in a Cd-polluted farmland in Hunan Agricultural University\u0026rsquo;s training base, Changsha City, Hunan Province, China. Experiment B: Ramie was planted in a Cd-polluted farmland in Liu yang City, Hunan Province, China. The lateral branches of ramie variety 171 were cut and propagated in June 2017. The plants were planted in the field in April 2017 and were mowed in December 2017. The ramie variety 171 was provided by the Ramie Research Institute of Hunan Agricultural University (Changsha, China).\u003c/p\u003e\n\u003cp\u003eSurface soil samples (0-20 cm) were taken from the test site, Then, the soil samples were air-dried and sieved through a 2-mm nylon screen to remove any debris before testing. Six air-dried soil samples were randomly taken to determine the physical-chemical properties. (GB15618-2018) The soil type was red soil, pH= 5.73, the average Cd content in soil was 3.27 mg kg\u003csup\u003e-1\u003c/sup\u003e, soil organic matter= 29.25 g kg\u003csup\u003e-1\u003c/sup\u003e, total nitrogen=1.56 g kg\u003csup\u003e-1\u003c/sup\u003e, total phosphorus= 0.51 g kg\u003csup\u003e-1\u003c/sup\u003e, total potassium=14.71 g kg\u003csup\u003e-1\u003c/sup\u003e。\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003e2.2 Field experiment\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eExperiment A and Experiment B were conducted at vigorous growing period. In experiment A, five plant growth regulators in different concentrations were sprayed into the positive and negative sides of ramie leaves in the corresponding plots. (Table 1) In experiment B, GA, KNO\u003csub\u003e3\u003c/sub\u003e and KH\u003csub\u003e2\u003c/sub\u003ePO\u003csub\u003e4\u0026nbsp;\u003c/sub\u003eare compounded in different concentrations was sprayed onto the positive and negative sides of ramie leaves in the corresponding plots. (Table 2) This procedure was repeated five times, once every 15 days, from April 19, 2018. To reduce the effect of direct sunlight and to prevent the agents from being washed away by the rain, the agents were administered on sunny mornings when the sun is not too strong.\u0026nbsp;\u003c/p\u003e\n\u003cp\u003eThe ramie grew in the soil for 125 days after transplantation and then harvested and divided into various parts for further processing. Before ramie was harvested, the agronomic traits of the ramie under each treatment were examined during the mature stage. A total of 10 plants were selected for measurement. A meter scale was used to measure the height of the plants. The diameter and thickness of the stems were measured using a micrometer. Ramet number was measured through manual calculation. The area of the leaves was calculated by measuring their length and width with a straightedge. The tissues were carefully washed with tap water and double-distilled water to ensure no dust or other undesirable materials remained on the surface of the samples. The tissues were dried in an oven at 60 \u0026plusmn; 5 \u0026deg;C for 4 days to ensure the constant weight. The weight of the samples was then measured, and the samples were crushed into powder for the Cd analysis.\u0026nbsp;\u003c/p\u003e\n\u003cp\u003eTake the soil in the rhizosphere of ramie，and then the soil samples were air dried, crushed gently, and passed through a 2-mm sieve prior to the Cd analysis for calculation of index.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003e2.3 Determination of Cd concentration\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eThe dried plant materials and soil samples were ground into powder and sieved, and 0.5 g samples were digested in mixed acid (HNO\u003csub\u003e3\u003c/sub\u003e + HClO\u003csub\u003e4\u003c/sub\u003e [3:1, v/v]).(Tang et al. \u003cspan class=\"CitationRef\"\u003e2015a\u003c/span\u003e) Cd content was determined using an atomic absorption spectrometer (SOLAAR M6). The linear fitting of the results of the samples measurements was 0.998 and the fitting degree of the equation was tested by chi square.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003e2.4 Statistical analysis\u003c/strong\u003e\u003c/p\u003e\u003cspan\u003e\n \u003cp\u003e2.4.1 Comparison of Ramie Field Performance with different treatments\u003c/p\u003e\n\u003c/span\u003e\n\u003cp\u003eField performance among different treatments were compared using ANOVA (analysis of variance) in SAS 9.4 software (SAS Institute, Cary, NC, United States). Plant data with different treatments were considered independent variables. The mean of each trait was tested at the p \u0026lt; 0.05 level and p \u0026lt; 0.01level using Duncan\u0026rsquo;s multiple range test. (The following is the same) Evaluation of the overall field performance is a multi-criteria decision-making process that involves many factors.\u003c/p\u003e\n\u003cp\u003eIn this study, a Membership function (MF) value and synthetic membership function (SMF) value were used to comprehensively express overall field performance. (Jin et al. \u003cspan class=\"CitationRef\"\u003e2020\u003c/span\u003e)The MF value of each field performance trait was calculated based on the following formula:\u003c/p\u003e\n\u003cdiv class=\"Equation\" id=\"Equa\"\u003e\n \u003cdiv class=\"mathdisplay\" id=\"FileID_Equa\" name=\"EquationSource\"\u003e$$yi \\left(k\\right) = \\left[xi \\right(k)-\\text{m}\\text{i}\\text{n} x (k\\left)\\right] / \\left[ \\text{m}\\text{a}\\text{x} x \\right(k) - \\text{m}\\text{i}\\text{n} x (k\\left)\\right] \\left(1\\right)$$\u003c/div\u003e\n\u003c/div\u003e\n\u003cp\u003eWhere \u003cem\u003eyi\u003c/em\u003e(k) represents the MF value of the k th field performance trait, \u003cem\u003exi\u003c/em\u003e(\u003cem\u003ek\u003c/em\u003e) denotes the field-recorded value of the kth field performance, and max \u003cem\u003ex\u003c/em\u003e(\u003cem\u003ek\u003c/em\u003e) and min \u003cem\u003ex\u003c/em\u003e(\u003cem\u003ek\u003c/em\u003e) represent the largest and smallest value of \u003cem\u003exi\u003c/em\u003e(\u003cem\u003ek\u003c/em\u003e), respectively.\u003c/p\u003e\n\u003cp\u003eThe SMF value of each treatment was calculated based on the following formula:\u003c/p\u003e\n\u003cdiv class=\"Equation\" id=\"Equb\"\u003e\n \u003cdiv class=\"mathdisplay\" id=\"FileID_Equb\" name=\"EquationSource\"\u003e$$\\sum _{\\text{i}=1}^{\\text{i}=\\text{n}}yi\\left(k\\right)=\\left[xi \\right(k) - \\text{m}\\text{i}\\text{n} x (k\\left)\\right] / \\left[\\text{m}\\text{a}\\text{x} x \\right(k) - \\text{m}\\text{i}\\text{n} x (k\\left) \\right(2)$$\u003c/div\u003e\n\u003c/div\u003e\n\u003cp\u003e2.4.2 Comparison of cadmium related indexes of Ramie with different treatments\u003c/p\u003e\n\u003cp\u003eThe accumulation and absorption of cadmium in Ramie with different treatments can be shown by many indexes. (Wei et al. \u003cspan class=\"CitationRef\"\u003e2012\u003c/span\u003e) The (BCF) value of each treatment was calculated based on the following formula:\u003c/p\u003e\n\u003cdiv class=\"Equation\" id=\"Equc\"\u003e\n \u003cdiv class=\"mathdisplay\" id=\"FileID_Equc\" name=\"EquationSource\"\u003e$$yBCF \\left(k\\right)= x \\text{p}\\text{a}\\text{r}\\text{t} \\text{o}\\text{f} \\text{p}\\text{l}\\text{a}\\text{n}\\text{t} \\left(k\\right) / x \\text{s}\\text{o}\\text{i}\\text{l} \\left(k\\right) \\left(3\\right)$$\u003c/div\u003e\n\u003c/div\u003e\n\u003cp\u003eWhere \u003cem\u003eyBCF\u003c/em\u003e (\u003cem\u003ek\u003c/em\u003e) the Cd bioconcentration factor value (BCF) of the k th treatments, \u003cem\u003ex\u003c/em\u003e part of plant (\u003cem\u003ek\u003c/em\u003e) denotes the cadmium concentration value of the \u003cem\u003ek\u003c/em\u003e th, and \u003cem\u003ex\u003c/em\u003e soil (\u003cem\u003ek\u003c/em\u003e) represent the cadmium concentration value of the \u003cem\u003ek\u003c/em\u003e th soil.\u003c/p\u003e\n\u003cp\u003eThe Cd transfer coefficient (TF) value of each treatment was calculated based on the following formula:\u003c/p\u003e\n\u003cdiv class=\"Equation\" id=\"Equd\"\u003e\n \u003cdiv class=\"mathdisplay\" id=\"FileID_Equd\" name=\"EquationSource\"\u003e$$yTF \\left(k\\right)= x \\text{a}\\text{b}\\text{o}\\text{v}\\text{e}\\text{g}\\text{r}\\text{o}\\text{u}\\text{n}\\text{d}\\left(k\\right) / x \\text{u}\\text{n}\\text{d}\\text{e}\\text{r}\\text{g}\\text{r}\\text{o}\\text{u}\\text{n}\\text{d}\\left(k\\right) \\left(4\\right)$$\u003c/div\u003e\n\u003c/div\u003e\n\u003cp\u003eWhere \u003cem\u003eyTF\u003c/em\u003e(\u003cem\u003ek\u003c/em\u003e) represents the Cd transfer coefficient value (TF) of the k th treatments, \u003cem\u003ex\u003c/em\u003e aboveground denotes the cadmium concentration value of the \u003cem\u003ek\u003c/em\u003e th aboveground, and \u003cem\u003ex\u003c/em\u003e underground (\u003cem\u003ek\u003c/em\u003e) represent the cadmium concentration value of the \u003cem\u003ek\u003c/em\u003e th soil.\u003c/p\u003e\n\u003cp\u003eThe Enrichment quantity value of each treatment was calculated based on the following formula:\u003c/p\u003e\n\u003cdiv class=\"Equation\" id=\"Eque\"\u003e\n \u003cdiv class=\"mathdisplay\" id=\"FileID_Eque\" name=\"EquationSource\"\u003e$$yEQ \\left(k\\right)=x \\text{b}\\text{i}\\text{o}\\text{m}\\text{a}\\text{s}\\text{s}\\left(k\\right) \\text{*} x \\text{C}\\text{d} \\text{c}\\text{o}\\text{n}\\text{t}\\text{e}\\text{n}\\text{t} (\\text{a}\\text{b}\\text{o}\\text{v}\\text{e}\\text{g}\\text{r}\\text{o}\\text{u}\\text{n}\\text{d}+ \\text{u}\\text{n}\\text{d}\\text{e}\\text{r}\\text{g}\\text{r}\\text{o}\\text{u}\\text{n}\\text{d}) \\left(k\\right) \\left(5\\right)$$\u003c/div\u003e\n\u003c/div\u003e\n\u003cp\u003eWhere \u003cem\u003eyEQ\u003c/em\u003e (\u003cem\u003ek\u003c/em\u003e) represents the enrichment quantity value of the \u003cem\u003ek\u003c/em\u003e th treatments, \u003cem\u003ex\u003c/em\u003e biomass(\u003cem\u003ek\u003c/em\u003e) denotes the biomass of the k th treatments, and \u003cem\u003ex\u003c/em\u003e Cd content (aboveground +underground) (\u003cem\u003ek\u003c/em\u003e) represent the \u003cem\u003ek\u003c/em\u003e th Cd content of the sum of aboveground and underground.\u003c/p\u003e\n\u003cp\u003e2.4.3 Correlation between cadmium related indexes and agronomic traits of ramie in different treatments\u003c/p\u003e\n\u003cp\u003eCorrelation analysis (CA analysis) was used to evaluate the relationship between the growth and development of ramie and the accumulation and absorption of Cd. Correlations between the ramie\u0026rsquo;s overall agronomic traits and the cadmium related indexes were performed using the CORR procedure in SAS 9.4 software. Pearson\u0026rsquo;s correlation coefficients and their significance were used to assess the strength of the correlations.\u003c/p\u003e\n\u003cp\u003eAll assays were made in triplicate. Graphs were drawn using GraphPad Prism 7.0 (GraphPad Software, San Diego, CA, USA).\u003c/p\u003e"},{"header":"3. Results","content":"\u003cp\u003e\u003cstrong\u003e3.1. Analysis of agronomic traits and enrichment quantity after plant growth regulator treatment and mixture of GA and foliar fertilizers\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003e3.1.1. Effects of plant growth regulators on agronomic traits and Cd enrichment\u003c/p\u003e\n\u003cp\u003eThe effects of plant growth regulator on the agronomic traits were evident. Plant growth varied in accordance with variety and concentration of plant growth regulators. Plant height, stem diameter, skin thickness, and leaf area were the main parameters influencing biomass. As shown in Table \u003cspan class=\"InternalRef\"\u003e3\u003c/span\u003e, GA-3 and PAs-3 treatments significantly increased plant height. For all treatments, plant height significantly decreased after plants were sprayed with ETH. All hormone treatments except the ETH treatments caused significant increases in biomass accumulation compared with the control 1(CK-1) treatment. The effect of plant growth regulators treatments on the leaf area, stem diameter, and skin thickness of ramie was negligible; the ETH-3 treatment was the only treatment to significantly reduce these measures in comparison with control.\u003c/p\u003e\n\u003cp\u003eCd enrichment is the overall capacity of ramie to adsorb Cd. Treatment with PAs achieved the most noticeable effect on Cd enrichment; Cd enrichment after treatment with all three concentrations of PAs was significantly higher than after CK-1 treatment. Cd enrichment after treatment with SA-1 and SA-3 was significantly higher than that after CK-1 treatment. The BR-1 and GA-2 treatments also increased Cd enrichment. However, Cd enrichment after the ETH-1, ETH-2, ETH-3 treatments was lower than that after CK-1 treatment.\u003c/p\u003e\n\u003cp\u003e3.1.2. Effects of GA and foliar fertilizer mixture on agronomic traits and Cd enrichment\u003c/p\u003e\n\u003cp\u003eThe GP and GN treatments positively affected the agronomic traits and Cd enrichment of ramie (Table \u003cspan class=\"InternalRef\"\u003e4\u003c/span\u003e). Plant height and biomass of ramie under GP-2, GP-3 and GN-2, GN-3 treatments were generally significantly higher than those under CK-2, but GP-1 and GN-1 treatments were significantly lower. Among the GW treatments, Cd enrichment after the GP-3 and GN-3 treatments was significantly higher than that after CK-2 treatment. The GP-1, GP-2, GP-3 and GN-1, GN-2, GN-3 reduced the biomass and Cd enrichment of ramie more than GW-1, GW-2, GW-3.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003e3.2 Effects of plant growth regulators on Cd content, Cd TF, and Cd BCF of ramie\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003e3.2.1 Effects of plant growth regulators on Cd content of ramie\u003c/p\u003e\n\u003cp\u003eCompared with the control, plant growth regulator treatments significantly increased the Cd content in the aboveground ramie. The Cd content of the aboveground ramie changed in accordance with type and concentration of plant growth regulators (Figure 1.a). The BR, GA, SA, ETH, and PAs plant growth regulator treatments increased the Cd content of the aboveground ramie. According to the results, the Cd content after treatments with various concentrations of plant growth regulators, except the BR-1, GA-3, SA-2, and SA-3 treatments, was considerably higher than that of the control group. The GA-1 and SA-1 treatments exerted the strongest effect; Cd content after these treatments was 3 times higher than that after CK-1 treatment. The Cd content of the aboveground ramie in the GA-1, GA-2, GA-3 group and PAs-1, PAs-2, PAs-3 group decreased as the concentration of GA and PAs increased. The Cd content of the aboveground ramie in the ETH-1, ETH-2, ETH-3 group exhibited the opposite effect. The Cd content of the aboveground ramie in the SA-1, SA-2, SA-3 group was similar to that of the BR-1, BR-2, BR-3 group; as the concentration of SA and BR increased, the Cd content of the aboveground ramie decreased at first and then increased.\u003c/p\u003e\n\u003cp\u003eThe plant growth regulators affected the Cd content of both the aboveground and the underground parts of ramie. The Cd content of the underground ramie after all treatments was generally lower than that of the control group (Figure 1.b), especially the Cd content of the groups treated with BR-2 and GA-1, which was 59.45% and 54.76% lower than that of the control, respectively. Similarly, the Cd content of the groups treated with GA-3, PAs-1, and PAs-2 was significantly lower than that of the control group. The Cd content of the underground ramie treated with BR decreased when the concentration of BR increased, which was contrary to the trend for the GA and SA treatments. The Cd content of the underground ramie after the ETH and PAs treatments did not change significantly.\u003c/p\u003e\n\u003cp\u003e3.2.2 Effects of plant growth regulators on Cd TF of ramie\u003c/p\u003e\n\u003cp\u003eCd TF refers to the ratio of Cd content of the aboveground part of ramie to that of the underground part. TF is an index used to evaluate the transportation of Cd from underground to aboveground parts of plants. Figure 2 shows that the Cd TF of ramie treated with lant growth regulators significantly increased. The TFs after the ETH-1, ETH-2, ETH-3 group treatment increased with an increasing concentration of ETH. By contrast, the TFs after the GA-1, GA-2, GA-3 group and PA-1, PA-2, PA-3 group treatment decreased as the concentration of the plant growth regulators increased. The TFs after the SA-1, SA-2, SA-3 group treatment decreased at first and then increased with the concentration of SA. The GA-1 treatment was the most effective and significantly stronger than CK-1, which yielded a TF greater than 2.\u003c/p\u003e\n\u003cp\u003e3.2.3 Effects of plant growth regulators on Cd BCF of ramie\u003c/p\u003e\n\u003cp\u003eA mount of heavy metals a plant absorbs and enriches from soil can be used as an indicator of the plant\u0026rsquo;s enrichment ability. The BCF of Cd is the ratio of the element content in a certain part of the plant to the corresponding element content in the soil. To a certain extent, the BCF of Cd reflects the degree of difficulty for an element to migrate through the soil\u0026ndash;plant system and indicates Cd enrichment in plants. As shown in Figure 3.a, the Cd BCF of the aboveground ramie after the GA-1, GA-2, GA-3 group, PA-1, PA-2, PA-3 group and SA-1, SA-2, SA-3 group treatments increased with an increase in the concentration of GA, PAs and SA. The Cd BCF of the aboveground ramie after the GA-1, PAs-1, SA-1 treatments and the GA-2 treatment was significantly higher than that of the control. However, the Cd BCF of the aboveground ramie after the SA-3 treatment was significantly lower than that of the control. The Cd BCF of the aboveground ramie after the ETH-1, ETH-2, ETH-3 group treatment increased at first and then decreased, and the Cd BCF of the aboveground ramie after the ETH-2 treatment was significantly higher than that of the CK-1. The Cd BCF of the aboveground ramie after the BR-1, BR-2, BR-3 group treatment exhibited the opposite behavior to that after the ETH-1, ETH-2, ETH-3 group treatment; the Cd BCF of the aboveground ramie after the BR-2 treatment was significantly lower than that of the CK-1.\u003c/p\u003e\n\u003cp\u003eThe Cd BCF of the underground ramie after all plant growth regulator treatments was generally significantly lower than that after CK-1 treatment. The Cd BCF of the underground ramie after the GA-1 and SA-1, GA-3 and SA-3, PAs-2, and PAs-3 treatments were significantly lower than that after CK-1 treatment and did not markedly change after the GA-2, SA-3 and PAs-1 treatments. The changes in the Cd BCF of the underground ramie upon increasing concentrations of BR and ETH were similar to those in the aboveground Cd BCF (Figure 3.b).\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003e3.3 Effects of GA and foliar fertilizer mixture on Cd content, Cd TF, and Cd BCF of ramie\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003e3.3.1 Effects of GA and foliar fertilizer mixture on Cd content of ramie\u003c/p\u003e\n\u003cp\u003eGA significantly increased the Cd content, TF, and BCF of the aboveground ramie (Figure 1-3). Because fertilizers composed of nitrogen and potassium are known to promote the growth and development of ramie, this study examined whether a mixture of nitrogen foliar fertilizer, potassium fertilizer, and GA could enhance the ability of ramie to absorb and enrich Cd. Figure 4.a shows that treatment with a mixture of GA mixed and foliar fertilizers did not significantly affect the Cd content of the aboveground ramie compared with CK-2 treatment, except the GN-1 treatment, which reduced the Cd content by 59.88%. The combination of GA and foliar fertilizer significantly reduced the Cd content of the aboveground ramie in comparison with GA alone.\u003c/p\u003e\n\u003cp\u003eThe combination of GA and foliar fertilizer did not significantly affect the Cd content of the underground ramie. The Cd content of the underground ramie after the GP-3 and GN-3 treatments was slightly higher than that after CK-2 treatment (Figure 4.b).\u003c/p\u003e\n\u003cp\u003e3.3.2 Effects of GA and foliar fertilizer mixture on Cd TF of ramie\u003c/p\u003e\n\u003cp\u003eWe discovered that unlike GA alone, the mixture of GA and fertilizers reduced the Cd TF of ramie. However, most compound treatments did not significantly affect the Cd TF in comparison with CK-2 treatment; only the GN-1 treatment significantly affected the Cd TF. The Cd TF after the GN-1 treatment was half that after CK-2 treatment (Figure \u003cspan class=\"InternalRef\"\u003e5\u003c/span\u003e).\u003c/p\u003e\n\u003cp\u003e3.3.3 Effects of GA and foliar fertilizer mixture on Cd BCF of ramie\u003c/p\u003e\n\u003cp\u003eThe Cd BCF of the aboveground ramie after the GP-1, GP-2, GP-3 group treatment and GN-1, GN-2, GN-3 group treatment were generally lower than that after the CK-2 and GW-1, GW-2, GW-3 group treatment, and the interaction between GP-1, GP-2, GP-3 group treatment and GN-1, GN-2, GN-3 group treatment made the Cd BCF of the aboveground ramie significantly lower than did the GW-1, GW-2, GW-3 group treatment with the same concentration of GA (Figure 6.a).\u003c/p\u003e\n\u003cp\u003eThe Cd BCF of the underground ramie after the GP-1, GP-2, GP-3 group treatment and GN-1, GN-2, GN-3 group treatment were generally higher than that after the GW-1, GW-2, GW-3 group treatment, but were not significantly different from that after CK-2 treatment, except for the GP-3 treatment (Figure 6.b). The Cd BCF of the underground ramie after the GP-2 treatment was not significantly different from that after the GW-2 treatment. Therefore, treatment with GA significantly reduces the Cd BCF of the underground part of ramie, but the mixture of GA of foliar fertilizer can negate this effect.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003e3.4 Correlation analysis of traits\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003e3.4.1 Effects of plant growth regulators on correlation\u003c/p\u003e\n\u003cp\u003eFigure 7 presents a significant correlation among various indicators after plant growth regulators treatment. For example, plant height was significantly correlated with leaf area and biomass. Leaf area and biomass were positively correlated with plant height, with correlation coefficients of 0.74 and 0.85 respectively. A significant positive correlation was observed between leaf area and biomass, with a correlation coefficient of 0.77. Aboveground Cd content was significantly correlated with Cd TF and aboveground Cd BCF. Cd TF and aboveground Cd BCF were positively correlated, with correlation coefficients of 0.82 and 0.84. A significant negative correlation was observed between soil Cd content, aboveground Cd BCF, and underground Cd BCF, with correlation coefficients of \u0026minus;0.74 and \u0026minus;0.79, respectively. The correlation coefficient of the significant positive correlation between Cd TF and aboveground Cd BCF was 0.73. The correlation coefficient of the significant positive correlation between aboveground Cd BCF and underground Cd BCF was 0.63. Cd enrichment had a significant positive correlation with plant height(0.72),leaf area (0.68), and biomass (0.88).\u0026nbsp;\u003c/p\u003e\n\u003cp\u003e3.4.2 Effects of GA and foliar fertilizers mixture on correlation\u003c/p\u003e\n\u003cp\u003eBiomass and Cd enrichments had a significantly positive correlation after treatment with GA and foliar fertilizer mixtures. (Figure \u003cspan class=\"InternalRef\"\u003e8\u003c/span\u003e) Aboveground Cd content was positively correlated with Cd TF (0.86) and aboveground Cd BCF (0.92) and negatively correlated with underground Cd BCF (\u0026minus;0.85) and underground Cd content (\u0026minus;0.71). A significant negative correlation was observed between soil Cd content and underground Cd content, with a correlation coefficient of \u0026minus;0.68. Cd TF was significantly and positively correlated with aboveground Cd BCF and negatively correlated with underground Cd BCF, with correlation coefficients of 0.95 and \u0026minus;0.86, respectively. Cd enrichment had a significant positive correlation with stem diameter (0.66), leaf area (0.71),andbiomass(0.90).\u003c/p\u003e"},{"header":"4. Discussion","content":"\u003cp\u003eTreating Cd soil pollution is an urgent task, and the phytoremediation technology-based approach can achieve superior results both economically and ecologically.(Wang et al. \u003cspan class=\"CitationRef\"\u003e2021b\u003c/span\u003e) Ramie is a strong candidate and can promote the green revolution. Planting ramie can not only promote the development of the textile industry but also prevent soil pollution from entering the food chain, which would positively affect human health.(Yaseen et al. \u003cspan class=\"CitationRef\"\u003e2016\u003c/span\u003e) Investigation of the physiological and molecular mechanisms of Cd in ramie is crucial to regulating the amount of Cd moving from soil to plants and repairing soil. For the short-distance transport of Cd through the roots, phytochelatin secretion and vacuolar partition via ion channels and transporters are the key elements in the absorption, transport, and accumulation of Cd; for long-distance transport, the loading and unloading of phloem is a crucial element in the transport and accumulation of Cd to ramie plants. The transport and accumulation of Cd plants also causes physiological responses to Cd stress in ramie plants, which can manifest as changes in plant growth regulators levels, photosynthesis, water absorption, and mineral element absorption.(Wang et al. \u003cspan class=\"CitationRef\"\u003e2021c\u003c/span\u003e, Yoneyama et al. \u003cspan class=\"CitationRef\"\u003e2015\u003c/span\u003e) This study examined the effects of several plant growth regulators and the combination of fertilizers and GA on ramie. The increases in the Cd TF of ramie after the hormone treatments may be explained by the following: plant growth regulators caused the increases in the number of physiological and biochemical molecules absorbing and carrying Cd and enhanced the Cd resistance of ramie, resulting in the Cd enrichment of the aboveground part of ramie.\u003c/p\u003e\n\u003cp\u003eGA, ETH, SA, BR, and PAs play a crucial role in alleviating abiotic stress and regulating the growth and development of plants.(Alcazar et al. \u003cspan class=\"CitationRef\"\u003e2020\u003c/span\u003e, Bajguz \u003cspan class=\"CitationRef\"\u003e2011\u003c/span\u003e) According to results of this study, SA, BR, and PA played an essential role in the transportation of Cd in both the aboveground and underground parts of ramie, and the effects of BR and PA were dependent on concentration and ramie part. GA is a plant growth stimulation hormone that regulates several physiological and biochemical processes, promotes growth and development, affects morphogenesis, and plays an essential role in the response to both biotic and abiotic stresses in plants.(Shu et al. \u003cspan class=\"CitationRef\"\u003e2018\u003c/span\u003e, Spence \u0026amp;Bais \u003cspan class=\"CitationRef\"\u003e2015\u003c/span\u003e) Studies have demonstrated that plant growth regulators such as SA, GA, and indole-3-acetic acid (IAA) can alleviate abiotic stresses.(Hussain et al. \u003cspan class=\"CitationRef\"\u003e2020\u003c/span\u003e, Jia et al. \u003cspan class=\"CitationRef\"\u003e2021\u003c/span\u003e, Saleem et al. \u003cspan class=\"CitationRef\"\u003e2015\u003c/span\u003e, Zhang et al. \u003cspan class=\"CitationRef\"\u003e2015\u003c/span\u003e) For example, the use of GA on leaves can reduce the uptake of nickel by mung bean plants, increase biomass, and promote growth. The importance of GA under abiotic stress has been well documented. This study confirmed that GA can promote the growth of ramie and improve its ability to absorb and enrich Cd. ETH can inhibit the growth of plants, and the results in subsection 3.1 indicated that ETH caused a decrease in biomass and Cd enrichment. Studies on other plants have demonstrated that ETH can promote leaf abscission and plant maturation and inhibit apical dominance but that spraying at certain stages may produce the opposite effects.(Schubert et al. \u003cspan class=\"CitationRef\"\u003e2019\u003c/span\u003e) The height of ramie decreased after ETH was applied, but the tillering and leaf area of ramie did not change significantly. ETH reduces the main components of biomass, thereby leading to a decrease of biomass. The enrichment of Cd in the plants decreased in accordance with changes in biomass.\u003c/p\u003e\n\u003cp\u003eN, P, and K are vital nutrients for plant growth. The addition of fertilizer to the cultivation process benefits the growth and development of crops. (Xia et al. \u003cspan class=\"CitationRef\"\u003e2020\u003c/span\u003e) The results of this study demonstrated that GA alone or in combination with KNO\u003csub\u003e3\u003c/sub\u003e or KH\u003csub\u003e2\u003c/sub\u003ePO\u003csub\u003e4\u003c/sub\u003e positively influenced the agronomic traits of ramie, indicating that the mixture of GA and fertilizer with N or P exhibited the same effects on the growth and development of ramie as in previous studies. In addition, treatment with GP (including GP-1, GP-2, GP-3) and GN (including GN-1, GN-2, GN-3) promoted the enrichment of Cd in ramie. This may be related to the physiological process ramie undergoes during heavy metal stress. A key regulator of plant growth and development, the functional site of GA at the cellular, tissue, and organ level of ramie is unknown.(Ubeda-Tomas et al. \u003cspan class=\"CitationRef\"\u003e2008\u003c/span\u003e) The site of application for GA on ramie can be a subject for future research. P +, K +, and Cd+ share the same transport pathway in plants. Although treatment with fertilizer promoted the growth and development of the plants, their Cd+ absorption and transport capacity decreased. Phosphates can increase the ionic strength of Cd adsorption.(Yan et al. \u003cspan class=\"CitationRef\"\u003e2015\u003c/span\u003e) Therefore, the decrease caused by GN (including GN-1, GN-2, GN-3)was more apparent than that caused by GP, especially in aboveground Cd content and aboveground BCF. The BCF after the GP (including GP-1, GP-2, GP-3) and GN (including GN-1, GN-2, GN-3) treatments was higher than that after the GW-1, GW-2, GW-3 group treatment, which may be related to the chelation of root exudates.\u003c/p\u003e\n\u003cp\u003ePlants\u0026rsquo; response to Cd stress is a complex physiological process involving ion transporters.(Lu et al. \u003cspan class=\"CitationRef\"\u003e2020\u003c/span\u003e) Studies on yeast(Mesquita et al. \u003cspan class=\"CitationRef\"\u003e2016\u003c/span\u003e), Arabidopsis(Zheng et al. \u003cspan class=\"CitationRef\"\u003e2018\u003c/span\u003e), and rice(Pan et al. \u003cspan class=\"CitationRef\"\u003e2021\u003c/span\u003e) have demonstrated that adenosine triphosphate\u0026ndash;binding cassette transporters, heavy metal\u0026ndash;associated transporters, and natural resistance\u0026ndash;associated macrophage protein transporters are involved in the response to Cd stress. The results of the study revealed common phenomena among the hormone treatments: the aboveground Cd content and Cd BCF increased; the underground Cd content and Cd BCF decreased; and the TF increased significantly after all hormone treatments. Traditionally a textile crop, ramie has high cellulose, hemicellulose, and lignin content in the phloem, which establishes the conditions for Cd accumulation. The pulp inside ramie contains a large number of vessels that can transport Cd \u003csup\u003e+\u003c/sup\u003e. Hormones promote the growth of the aboveground part of ramie, the transport of Cd, and the accumulation of Cd in the underground part of the plant. Liu (Liu et al. \u003cspan class=\"CitationRef\"\u003e2021\u003c/span\u003e) discovered that high concentrations of endogenous ETH delayed the formation of an ectoblast barrier and promoted the accumulation of Cd in the root ectoblasts. Studies by Neumann(Neumann \u003cspan class=\"CitationRef\"\u003e2015\u003c/span\u003e) have demonstrated that ETH-mediated responses usually have high genotypic variability and may partially share common pathways under certain nutritional constraints. Although the biomass and Cd enrichment of ramie decreased after the ETH treatment, the Cd content and BCF of the aboveground ramie increased under high TFs.\u003c/p\u003e"},{"header":"5. Conclusions","content":"\u003cp\u003ePlant growth regulators and foliar fertilizers increased the Cd content of the aboveground ramie, reduced the Cd content of the underground ramie, and increased the TF. Among them, GA-1 increased the Cd content of the aboveground ramie to 3 times more than that of the control and reduced the Cd content of the underground ramie by 54.76%. Salicylic acid (SA) increased the Cd content of the aboveground ramie to 3 times more than that of the control. The combination of GA and foliar fertilizer reduced the Cd content of the aboveground and underground ramie and the TF and BCF of the underground ramie. After the hormones were sprayed, the TF of ramie had a significant positive correlation with the Cd content of the aboveground ramie; the BCF of the aboveground ramie had a significant positive correlation with the Cd content and TF of the aboveground ramie. In actual production, the proper concentration of PAs, GA, SA and BR can be sprayed during the prosperous period of ramie, which will increase the biomass of ramie and increase the efficiency of cadmium enrichment of ramie. It is generally not recommended to mix plant regulators and foliar fertilizers.\u003c/p\u003e"},{"header":"Declarations","content":"\u003cp\u003e\u003cstrong\u003eAvailability of data and materials: \u003c/strong\u003eAll data generated or analyzed during this study are included in this published article.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eFunding\u003c/strong\u003e\u003cstrong\u003e:\u003c/strong\u003e This research was funded by Key R \u0026amp; D Program of Hunan Province, grant number 2022NK2017. \u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eAuthor information\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eAffiliation\u003c/strong\u003e\u003cstrong\u003es\u003c/strong\u003e \u003c/p\u003e\n\u003cp\u003eRamie Research Institute (Hunan Agricultural University), Changsha, 410128, China\u003c/p\u003e\n\u003cp\u003eKey Laboratory of germplasm resources innovation and utilization, Changsha, 410128, China\u003c/p\u003e\n\u003cp\u003eWenxian Peng, Yejun He, Si He, Jinfeng Luo, Yi Zeng, Xiaoyang Zhang, Yingyi Huo, Yucheng Jie, Hucheng Xing\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eAuthors Contributions\u003c/strong\u003e\u003cstrong\u003e:\u003c/strong\u003e Conceptualization, Wenxian.Peng. and Yejun.He.; methodology, Hucheng. Xing.; software, Wenxian. Peng. and Yejun.He.; validation, Wenxian.Peng.; investigation, Xiaoyang. Zhang.; resources, Yucheng. Jie.; data curation, Jinfeng. Luo. and Yi. Zeng.; writing-original draft preparation, Wenxian.Peng.; writing-review and editing, Wenxian.Peng., Si.He. and Yingyi.Huo. ; visualization, Wenxian.Peng.; supervision, Hucheng. Xing.; project administration, Hucheng. Xing.; funding acquisition, Hucheng. Xing. All authors have read and agreed to the published version of the manuscript.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eCorresponding author\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eCorrespondence to Hucheng Xing \u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eEthics declarations\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eEthics approval\u003c/strong\u003e\u003cstrong\u003e: \u003c/strong\u003eThe manuscripts reporting studies are not applicable for human participants, human data, or human tissue. The manuscript does not contain any individual person\u0026rsquo;s data in any form.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eConsent to participate\u003c/strong\u003e: Not applicable.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eConsent to Publish\u003c/strong\u003e：All authors agree to publish.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eCompeting interests\u003c/strong\u003e：The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. \u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eAcknowledgements\u003c/strong\u003e: Many thanks to the editor and reviewers for telling us how to make the manuscript more completed and better. Thanks to Hunan Agricultural University for providing the environment for the successful completion of this work.\u003c/p\u003e"},{"header":"References","content":"\u003col\u003e\u003cli\u003e\u003cspan\u003eAlcazar R, Bueno M, Tiburcio AF (2020) : Polyamines: Small Amines with Large Effects on Plant Abiotic Stress Tolerance. Cells 9\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eAli N, Hadi F (2015) Phytoremediation of cadmium improved with the high production of endogenous phenolics and free proline contents in Parthenium hysterophorus plant treated exogenously with plant growth regulator and chelating agent. Environ Sci Pollut Res Int 22:13305\u0026ndash;13318\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eAtafar Z, Mesdaghinia A, Nouri J, Homaee M, Yunesian M, Ahmadimoghaddam M, Mahvi AH (2010) Effect of fertilizer application on soil heavy metal concentration. 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Environ Pollut 264:114751\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eXue D, Jiang H, Deng X, Zhang X, Wang H, Xu X, Hu J, Zeng D, Guo L, Qian Q (2014) Comparative proteomic analysis provides new insights into cadmium accumulation in rice grain under cadmium stress. J Hazard Mater 280:269\u0026ndash;278\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eYadav T, Kumar A, Yadav RK, Yadav G, Kumar R, Kushwaha M (2020) Salicylic acid and thiourea mitigate the salinity and drought stress on physiological traits governing yield in pearl millet- wheat. Saudi J Biol Sci 27:2010\u0026ndash;2017\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eYalpani N, Raskin I (1993) Salicylic acid: a systemic signal in induced plant disease resistance. Trends Microbiol 1:88\u0026ndash;92\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eYan Y, Zhou YQ, Liang CH (2015) : Evaluation of phosphate fertilizers for the immobilization of Cd in contaminated soils.PloS one10, e0124022\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eYang B, Zhou M, Shu WS, Lan CY, Ye ZH, Qiu RL, Jie YC, Cui GX, Wong MH (2010) Constitutional tolerance to heavy metals of a fiber crop, ramie (Boehmeria nivea), and its potential usage. Environ Pollut 158:551\u0026ndash;558\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eYang Y, Shen Q (2020) Phytoremediation of cadmium-contaminated wetland soil with Typha latifolia L. and the underlying mechanisms involved in the heavy-metal uptake and removal. Environ Sci Pollut Res Int 27:4905\u0026ndash;4916\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eYaseen M, Aziz MZ, Jafar AA, Naveed M, Saleem M (2016) Use of textile waste water along with liquid NPK fertilizer for production of wheat on saline sodic soils. Int J Phytoremediation 18:502\u0026ndash;508\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eYoneyama T, Ishikawa S, Fujimaki S (2015) Route and Regulation of Zinc, Cadmium, and Iron Transport in Rice Plants (Oryza sativa L.) during Vegetative Growth and Grain Filling: Metal Transporters, Metal Speciation, Grain Cd Reduction and Zn and Fe Biofortification. Int J Mol Sci 16:19111\u0026ndash;19129\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eZamani S, Naderi MR, Soleymani A, Nasiri BM (2020) : Sunflower (Helianthus annuus L.) biochemical properties and seed components affected by potassium fertilization under drought conditions. Ecotoxicology and environmental safety 190\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eZeng GM, Wan J, Huang DL, Hu L, Huang C, Cheng M, Xue WJ, Gong XM, Wang RZ, Jiang DN (2017) Precipitation, adsorption and rhizosphere effect: The mechanisms for Phosphate-induced Pb immobilization in soils-A review. J Hazard Mater 339:354\u0026ndash;367\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eZhang P, Ma YH, Zhang ZY, He X, Li YY, Zhang J, Zheng LR, Zhao YL (2015) Species-specific toxicity of ceria nanoparticles to Lactuca plants. Nanotoxicology 9:1\u0026ndash;8\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eZhao J, Shi GX, Yuan QH (2008) : Polyamines content and physiological and biochemical responses to ladder concentration of nickel stress in Hydrocharis dubia (Bl.) Backer leaves. Biometals: an international journal on the role of metal ions in biology, biochemistry, and medicine 21, 665-674\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eZhao WQ, Dong HR, Zhou ZG, Wang YH, Hu W (2020) Potassium (K) application alleviates the negative effect of drought on cotton fiber strength by sustaining higher sucrose content and carbohydrates conversion rate. Plant Physiol Bioch 157:105\u0026ndash;113\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eZhao XL, Luan MB, Qiu CS, Guo Y, Long SH, Wang YF, Qiu HJ (2021) : Analysis of the potential of 165 ramie germplasms to be used for cadmium-contamination remediation.Ind Crop Prod171\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eZheng X, Chen L, Li XF (2018) : Arabidopsis and rice showed a distinct pattern in ZIPs genes expression profile in response to Cd stress.Botanical Studies59\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eZhu HH, Chen L, Xing W, Ran SM, Wei ZH, Amee M, Wassie M, Niu H, Tang DY, Sun J, Du DY, Yao J, Hou HB, Chen K (2020) : Phytohormones-induced senescence efficiently promotes the transport of cadmium from roots into shoots of plants: A novel strategy for strengthening of phytoremediation.Journal of hazardous materials388\u003c/span\u003e\u003c/li\u003e\u003c/ol\u003e"},{"header":"Tables","content":"\u003cp\u003eTable 1. Concentrations of different plant growth regulators.\u003c/p\u003e\n\u003cdiv class=\"gridtable\"\u003e\n \u003ctable border=\"1\" id=\"Tabb\"\u003e\n \u003ctbody\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003eExperiment\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003eTreatments\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003econcentration\u003c/div\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\" rowspan=\"14\"\u003e\n \u003cdiv class=\"SimplePara\"\u003eA\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003eCK-1\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e0 mg L\u003csup\u003e\u0026minus;1\u003c/sup\u003e\u003c/div\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003eGA-1\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e50mg L\u003csup\u003e\u0026minus;1\u003c/sup\u003e\u003c/div\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003eGA-2\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e100mg L\u003csup\u003e\u0026minus;1\u003c/sup\u003e\u003c/div\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003eGA-3\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e200mg L\u003csup\u003e\u0026minus;1\u003c/sup\u003e\u003c/div\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003eETH-1\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e50mg L\u003csup\u003e\u0026minus;1\u003c/sup\u003e\u003c/div\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003eETH-2\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e100mg L\u003csup\u003e\u0026minus;1\u003c/sup\u003e\u003c/div\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003eETH-3\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e200mg L\u003csup\u003e\u0026minus;1\u003c/sup\u003e\u003c/div\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003eSA-1\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e50mg L\u003csup\u003e\u0026minus;1\u003c/sup\u003e\u003c/div\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003eSA-2\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e100mg L\u003csup\u003e\u0026minus;1\u003c/sup\u003e\u003c/div\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003eSA-3\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e200mg L\u003csup\u003e\u0026minus;1\u003c/sup\u003e\u003c/div\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003ePAs-1\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e0.1mmol\u003c/div\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003ePAs-2\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e1mmol\u003c/div\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003ePAs-3\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e10mmol\u003c/div\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003eBR-1\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e0.1mg L\u003csup\u003e\u0026minus;1\u003c/sup\u003e\u003c/div\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003eBR-2\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e1mg L\u003csup\u003e\u0026minus;1\u003c/sup\u003e\u003c/div\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003eBR-3\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e10mg L\u003csup\u003e\u0026minus;1\u003c/sup\u003e\u003c/div\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003c/tbody\u003e\n \u003c/table\u003e\n\u003c/div\u003e\n\u003cp\u003eNote: GA represents gibberellin, ETH represents ethylene, SA represents salicylic acid, PAs represents polyamine, BR represents Brassinolide, the number 1,2 and 3 indicates the increase of different treatment concentrations, respectively.\u003c/p\u003e\n\u003cp\u003eTable 2. Concentrations of different plant growth regulator and fertilizers.\u003c/p\u003e\n\u003cdiv class=\"SimplePara\"\u003e\n \u003cdiv class=\"gridtable\"\u003e\n \u003ctable border=\"1\" id=\"Tabc\"\u003e\n \u003ctbody\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003eExperiment\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003eTreatments\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003econcentration\u003c/div\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\" rowspan=\"10\"\u003e\n \u003cdiv class=\"SimplePara\"\u003eB\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003eCK-2\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e0 mg L\u003csup\u003e\u0026minus;1\u003c/sup\u003e+0%\u003c/div\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003eGW-1\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e50mg L\u003csup\u003e\u0026minus;1\u003c/sup\u003e+0%\u003c/div\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003eGW-2\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e100mg L\u003csup\u003e\u0026minus;1\u003c/sup\u003e+0%\u003c/div\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003eGW-3\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e200mg L\u003csup\u003e\u0026minus;1\u003c/sup\u003e+0%\u003c/div\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003eGP-1\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e50mg L\u003csup\u003e\u0026minus;1\u003c/sup\u003e+0.2%\u003c/div\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003eGP-2\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e100mg L\u003csup\u003e\u0026minus;1\u003c/sup\u003e+0.4%\u003c/div\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003eGP-3\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e200mg L\u003csup\u003e\u0026minus;1\u003c/sup\u003e+0.6%\u003c/div\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003eGN-1\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e50mg L\u003csup\u003e\u0026minus;1\u003c/sup\u003e+1%\u003c/div\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003eGN-2\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e100mg L\u003csup\u003e\u0026minus;1\u003c/sup\u003e+1.5%\u003c/div\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003eGN-3\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e200mg L\u003csup\u003e\u0026minus;1\u003c/sup\u003e+2%\u003c/div\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003c/tbody\u003e\n \u003c/table\u003e\n \u003c/div\u003e\n\u003c/div\u003e\n\u003cp\u003eNote: GW: GA+ Water; GP: GA+ KH\u003csub\u003e2\u003c/sub\u003ePO\u003csub\u003e4\u003c/sub\u003e; GN: GA+KNO\u003csub\u003e3\u003c/sub\u003e; The number 1,2 and 3 indicates the increase of different treatment concentrations, respectively.\u003c/p\u003e\n\u003cdiv class=\"gridtable\"\u003e\n \u003ctable border=\"1\" id=\"Tab1\"\u003e\n \u003ccaption language=\"En\"\u003e\n \u003cdiv class=\"CaptionNumber\"\u003eTable 3\u003c/div\u003e\n \u003cdiv class=\"CaptionContent\"\u003e\n \u003cdiv class=\"SimplePara\"\u003eAgronomic traits and Cd enrichment of ramie under different hormones\u003c/div\u003e\n \u003c/div\u003e\n \u003c/caption\u003e\n \u003cthead\u003e\n \u003ctr\u003e\n \u003cth align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003eTreatments\u003c/div\u003e\n \u003c/th\u003e\n \u003cth align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003ePlant height\u003c/div\u003e\n \u003cdiv class=\"SimplePara\"\u003e(cm)\u003c/div\u003e\n \u003c/th\u003e\n \u003cth align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003eStem diameter\u003c/div\u003e\n \u003cdiv class=\"SimplePara\"\u003e(mm)\u003c/div\u003e\n \u003c/th\u003e\n \u003cth align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003eSkin thickness\u003c/div\u003e\n \u003cdiv class=\"SimplePara\"\u003e(mm)\u003c/div\u003e\n \u003c/th\u003e\n \u003cth align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003eLeaf area\u003c/div\u003e\n \u003cdiv class=\"SimplePara\"\u003e(cm\u003csup\u003e2\u003c/sup\u003e)\u003c/div\u003e\n \u003c/th\u003e\n \u003cth align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003eBiomass\u003c/div\u003e\n \u003cdiv class=\"SimplePara\"\u003e(kg ha\u003csup\u003e\u0026minus;1\u003c/sup\u003e)\u003c/div\u003e\n \u003c/th\u003e\n \u003cth align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003eCd enrichment\u003c/div\u003e\n \u003cdiv class=\"SimplePara\"\u003e(mg ha\u003csup\u003e\u0026minus;1\u003c/sup\u003e)\u003c/div\u003e\n \u003c/th\u003e\n \u003c/tr\u003e\n \u003c/thead\u003e\n \u003ctbody\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003eCK-1\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e212.07\u0026plusmn;5.6DE\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e11.45\u0026plusmn;0.58A\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e0.68\u0026plusmn;0.01AB\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e228.93\u0026plusmn;4.94AB\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e1659.24\u0026plusmn;19.09I\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e22709.46\u0026plusmn;70.71I\u003c/div\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003eBR-1\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e215.03\u0026plusmn;2.87DE\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e11.02\u0026plusmn;1.26A\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e0.68\u0026plusmn;0.06AB\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e230.73\u0026plusmn;1.37AB\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e2379.69\u0026plusmn;72.11E\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e43048.59\u0026plusmn;173.46E\u003c/div\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003eBR-2\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e217.30\u0026plusmn;5.28CDE\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e10.87\u0026plusmn;0.70A\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e0.71\u0026plusmn;0.10AB\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e222.52\u0026plusmn;5.11AB\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e2378.18\u0026plusmn;79.00E\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e23480.56\u0026plusmn;74.83E\u003c/div\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003eBR-3\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e221.70\u0026plusmn;5.86BCDE\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e11.64\u0026plusmn;0.91A\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e0.74\u0026plusmn;0.06AB\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e220.67\u0026plusmn;13.82B\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e2339.15\u0026plusmn;43.59EF\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e34640.09\u0026plusmn;71.18EF\u003c/div\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003eETH-1\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e145.30\u0026plusmn;3.82F\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e12.94\u0026plusmn;0.64A\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e0.68\u0026plusmn;0.04AB\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e183.24\u0026plusmn;8.37C\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e1025.15\u0026plusmn;8.89J\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e15824.90\u0026plusmn;142.77J\u003c/div\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003eETH-2\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e132.05\u0026plusmn;2.60G\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e12.39\u0026plusmn;1.32A\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e0.75\u0026plusmn;0.12AB\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e159.02\u0026plusmn;5.07D\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e965.19\u0026plusmn;25.98J\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e14297.68\u0026plusmn;72.57J\u003c/div\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003eETH-3\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e132.41\u0026plusmn;3.58G\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e11.90\u0026plusmn;1.06A\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e0.72\u0026plusmn;0.08AB\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e128.65\u0026plusmn;3.93E\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e1060.80\u0026plusmn;88.88J\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e17588.06\u0026plusmn;367.99J\u003c/div\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003eGA-1\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e212.00\u0026plusmn;3.00DE\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e12.10\u0026plusmn;0.69A\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e0.73\u0026plusmn;0.11AB\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e161.74\u0026plusmn;3.78D\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e1859.60\u0026plusmn;46.16H\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e28856.40\u0026plusmn;132.63H\u003c/div\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003eGA-2\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e228.00\u0026plusmn;4.35BC\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e12.02\u0026plusmn;0.75A\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e0.84\u0026plusmn;0.12A\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e195.03\u0026plusmn;7.10C\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e2019.60\u0026plusmn;20.52G\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e38068.73\u0026plusmn;500.58G\u003c/div\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003eGA-3\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e249.33\u0026plusmn;4.62A\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e12.80\u0026plusmn;0.60A\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e0.67\u0026plusmn;0.04AB\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e200.62\u0026plusmn;9.68C\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e2645.83\u0026plusmn;109.34D\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e32349.68\u0026plusmn;146.97D\u003c/div\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003ePAs-1\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e219.73\u0026plusmn;1.99BCDE\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e11.82\u0026plusmn;1.19A\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e0.69\u0026plusmn;0.06AB\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e224.50\u0026plusmn;7.87AB\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e2568.07\u0026plusmn;90.14D\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e40748.15\u0026plusmn;297.18D\u003c/div\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003ePAs-2\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e223.27\u0026plusmn;1.16BCD\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e12.35\u0026plusmn;1.26A\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e0.70\u0026plusmn;0.02AB\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e218.02\u0026plusmn;5.06B\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e3304.81\u0026plusmn;86.69B\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e49685.24\u0026plusmn;362.86B\u003c/div\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003ePAs-3\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e241.87\u0026plusmn;0.29A\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e11.67\u0026plusmn;1.17A\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e0.83\u0026plusmn;0.19A\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e227.30\u0026plusmn;12.26AB\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e3980.73\u0026plusmn;22.68A\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e58875.00\u0026plusmn;711.80A\u003c/div\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003eSA-1\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e209.80\u0026plusmn;2.33E\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e11.43\u0026plusmn;1.11A\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e0.69\u0026plusmn;0.07AB\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e220.34\u0026plusmn;3.94B\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e2209.42\u0026plusmn;69.28F\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e36772.11\u0026plusmn;432.05F\u003c/div\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003eSA-2\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e220.27\u0026plusmn;0.93BCDE\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e11.83\u0026plusmn;1.50A\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e0.58\u0026plusmn;0.09B\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e196.79\u0026plusmn;6.54C\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e2254.69\u0026plusmn;20.00EF\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e21772.79\u0026plusmn;285.77EF\u003c/div\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003eSA-3\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e230.03\u0026plusmn;13.83B\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e11.91\u0026plusmn;1.71A\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e0.80\u0026plusmn;0.17AB\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e236.02\u0026plusmn;5.82A\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e2827.55\u0026plusmn;54.44C\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e35542.30\u0026plusmn;216.07FG\u003c/div\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\" rowspan=\"2\"\u003e\n \u003cdiv class=\"SimplePara\"\u003eTreatment\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003eF\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; P\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003eF\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; P\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003eF\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;P\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003eF\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; P\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003eF\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; P\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003eF\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; P\u003c/div\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e164. 90\u0026nbsp; \u0026nbsp; \u0026nbsp; ***\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e0.82\u0026nbsp; \u0026nbsp; \u0026nbsp;0.65\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e1.35\u0026nbsp; \u0026nbsp; 0.23\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e56.18\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;***\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e515.02\u0026nbsp; \u0026nbsp; \u0026nbsp; ***\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e3284.93\u0026nbsp; \u0026nbsp; \u0026nbsp; ***\u003c/div\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003c/tbody\u003e\n \u003ctfoot\u003e\n \u003ctr\u003e\n \u003ctd colspan=\"7\"\u003eNote: The same letters within a column indicate no significant differences (P \u0026gt; 0.01) among the treatments and CK-1. Values are means \u0026plusmn; SD (n = 3). ***, P \u0026lt; 0.001; **, P \u0026lt;0.01; *, P \u0026lt; 0.05., Duncan\u0026rsquo;s multiple range test.\u003c/td\u003e\n \u003c/tr\u003e\n \u003c/tfoot\u003e\n \u003c/table\u003e\n\u003c/div\u003e\n\u003cdiv class=\"gridtable\"\u003e\n \u003ctable border=\"1\" id=\"Tab2\"\u003e\n \u003ccaption language=\"En\"\u003e\n \u003cdiv class=\"CaptionNumber\"\u003eTable 4\u003c/div\u003e\n \u003cdiv class=\"CaptionContent\"\u003e\n \u003cdiv class=\"SimplePara\"\u003eAgronomic traits and Cd enrichment of ramie under different hormones and fertilizers\u003c/div\u003e\n \u003c/div\u003e\n \u003c/caption\u003e\n \u003cthead\u003e\n \u003ctr\u003e\n \u003cth align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003eTreatments\u003c/div\u003e\n \u003c/th\u003e\n \u003cth align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003ePlant height\u003c/div\u003e\n \u003cdiv class=\"SimplePara\"\u003e(cm)\u003c/div\u003e\n \u003c/th\u003e\n \u003cth align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003eStem diameter\u003c/div\u003e\n \u003cdiv class=\"SimplePara\"\u003e(mm)\u003c/div\u003e\n \u003c/th\u003e\n \u003cth align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003eSkin thickness\u003c/div\u003e\n \u003cdiv class=\"SimplePara\"\u003e(mm)\u003c/div\u003e\n \u003c/th\u003e\n \u003cth align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003eLeaf area\u003c/div\u003e\n \u003cdiv class=\"SimplePara\"\u003e(cm\u003csup\u003e2\u003c/sup\u003e)\u003c/div\u003e\n \u003c/th\u003e\n \u003cth align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003eBiomass\u003c/div\u003e\n \u003cdiv class=\"SimplePara\"\u003e(kg ha\u003csup\u003e\u0026minus;1\u003c/sup\u003e)\u003c/div\u003e\n \u003c/th\u003e\n \u003cth align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003eCd enrichment\u003c/div\u003e\n \u003cdiv class=\"SimplePara\"\u003e(mg ha\u003csup\u003e\u0026minus;1\u003c/sup\u003e)\u003c/div\u003e\n \u003c/th\u003e\n \u003c/tr\u003e\n \u003c/thead\u003e\n \u003ctbody\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003eCK-2\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e183.8\u0026plusmn;4.44D\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e10.25\u0026plusmn;0.40AB\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e0.62\u0026plusmn;0.03A\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e236.04\u0026plusmn;3.72D\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e752.62\u0026plusmn;12.30G\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e6894.00\u0026plusmn;149.52E\u003c/div\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003eGP-1\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e171.2\u0026plusmn;1.97E\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e8.73\u0026plusmn;0.67B\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e0.59\u0026plusmn;0.07A\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e201.55\u0026plusmn;5.73E\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e1005.32\u0026plusmn;6.65F\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e7144.47\u0026plusmn;100.33E\u003c/div\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003eGP-2\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e219.3\u0026plusmn;3.52B\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e11.48\u0026plusmn;0.59A\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e0.74\u0026plusmn;0.10A\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e258.05\u0026plusmn;4.41C\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e1336.27\u0026plusmn;6.81D\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e11505.28\u0026plusmn;818.62C\u003c/div\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003eGP-3\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e225.57\u0026plusmn;4.55AB\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e12.28\u0026plusmn;0.48A\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e0.66\u0026plusmn;0.09A\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e272.58\u0026plusmn;3.84AB\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e1449.54\u0026plusmn;49.46C\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e15198.43\u0026plusmn;694.29B\u003c/div\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003eGN-1\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e182.68\u0026plusmn;3.83D\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e10.38\u0026plusmn;0.44AB\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e0.68\u0026plusmn;0.10A\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e224.24\u0026plusmn;6.61D\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e1153.65\u0026plusmn;45.72E\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e8540.86\u0026plusmn;224.84D\u003c/div\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003eGN-2\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e226.20\u0026plusmn;5.00AB\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e12.37\u0026plusmn;1.49A\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e0.73\u0026plusmn;0.01A\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e265.71\u0026plusmn;5.69BC\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e1398.06\u0026plusmn;4.06CD\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e10308.36\u0026plusmn;617.67C\u003c/div\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003eGN-3\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e231.43\u0026plusmn;3.19A\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e11.92\u0026plusmn;0.39A\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e0.75\u0026plusmn;0.09A\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e281.76\u0026plusmn;7.61A\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e1456.82\u0026plusmn;42.55C\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e15689.95\u0026plusmn;34.72B\u003c/div\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003eGW-1\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e206.67\u0026plusmn;4.38C\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e12.01\u0026plusmn;0.53A\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e0.7\u0026plusmn;0.09A\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e275.8\u0026plusmn;6.57AB\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e1629.20\u0026plusmn;81.64B\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e15444.82\u0026plusmn;867.03B\u003c/div\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003eGW-2\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e189.03\u0026plusmn;6.83D\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e11.92\u0026plusmn;1.47A\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e0.7\u0026plusmn;0.04A\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e256.23\u0026plusmn;3.79C\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e2325.11\u0026plusmn;49.97A\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e19608.43\u0026plusmn;48.11A\u003c/div\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003eGW-3\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e218.00\u0026plusmn;2.62B\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e10.72\u0026plusmn;0.68AB\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e0.56\u0026plusmn;0.08A\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e275.13\u0026plusmn;7.71AB\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e1626.20\u0026plusmn;31.61B\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e14240.09\u0026plusmn;876.31B\u003c/div\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\" rowspan=\"2\"\u003e\n \u003cdiv class=\"SimplePara\"\u003eTreatment\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003eF\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; P\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003eF\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;P\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003eF\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; P\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003eF\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; P\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003eF\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; P\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003eF\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;P\u003c/div\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e78.27\u0026nbsp; \u0026nbsp; \u0026nbsp;***\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e6.12\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;***\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e2.26\u0026nbsp; \u0026nbsp;0.06\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e61.76\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;***\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e318.68\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;***\u003c/div\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cdiv class=\"SimplePara\"\u003e153.03\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;***\u003c/div\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003c/tbody\u003e\n \u003ctfoot\u003e\n \u003ctr\u003e\n \u003ctd colspan=\"7\"\u003eNote: The same letters within a column indicate no significant differences (P \u0026gt; 0.01) among the treatments and CK. Values are means \u0026plusmn; SD (n = 3). ***, P \u0026lt; 0.001; **, P \u0026lt;0.01; *, P \u0026lt; 0.05., Duncan\u0026rsquo;s multiple range test.\u003c/td\u003e\n \u003c/tr\u003e\n \u003c/tfoot\u003e\n \u003c/table\u003e\n\u003c/div\u003e"}],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":true,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":true,"highlight":"","institution":"","isAcceptedByJournal":false,"isAuthorSuppliedPdf":false,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":false,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true},"keywords":"Ramie, plant growth regulator, Fertilizer, Cadmium, Gibberellin, Phytoextraction","lastPublishedDoi":"10.21203/rs.3.rs-1197456/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-1197456/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003e(1) Background: As a enrichment plant, ramie can be used for the phytoremediation of cadmium (Cd)-contaminated soil. However, it is worth exploring the role of plant growth regulators and foliar fertilizers in the process of plant growth and development and Cd adsorption. \u003c/p\u003e\u003cp\u003e(2) Methods: By measuring the agronomic traits, Cd content of aboveground and underground ramie, calculating the Cd transfer coefficient (TF) and Cd bioconcentration factors (BCF), and the correlation between various indicators. This study examined the effects of plant growth regulators and foliar fertilizers on ramie’s capacity for Cd accumulation and transportation, \u003c/p\u003e\u003cp\u003e(3) Results: Plant growth regulators and foliar fertilizers increased the Cd content of the aboveground ramie, reduced the Cd content of the underground ramie, and increased the TF. Among them, GA-1 increased the Cd content of the aboveground ramie to 3 times more than that of the control and reduced the Cd content of the underground ramie by 54.76%. Salicylic acid (SA) increased the Cd content of the aboveground ramie to 3 times more than that of the control. The combination of GA and foliar fertilizer reduced the Cd content of the aboveground and underground ramie and the TF and BCF of the underground ramie. After the hormones were sprayed, the TF of ramie had a significant positive correlation with the Cd content of the aboveground ramie; the BCF of the aboveground ramie had a significant positive correlation with the Cd content and TF of the aboveground ramie. \u003c/p\u003e\u003cp\u003e(4) Conclusions: The results indicate that Brassinolide (BR), gibberellin (GA), ethephon (ETH), polyamines (PAs), and salicylic acid (SA) have different effects on the enrichment and transport of Cd in ramie. This study provided an effective method to improve the capacity for ramie to adsorb heavy metals during cultivation.\u003c/p\u003e","manuscriptTitle":"Exogenous plant growth regulator and foliar fertilizers for phytoextraction of cadmium with Boehmeria nivea [L.] Gaudich from contaminated field soil.","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2022-02-25 14:46:10","doi":"10.21203/rs.3.rs-1197456/v1","editorialEvents":[{"type":"communityComments","content":0}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"b3d1e1e6-e14a-4dd6-9c84-6bc93e99cc91","owner":[],"postedDate":"February 25th, 2022","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"posted","subjectAreas":[],"tags":[],"updatedAt":"2023-01-16T09:14:23+00:00","versionOfRecord":[],"versionCreatedAt":"2022-02-25 14:46:10","video":"","vorDoi":"","vorDoiUrl":"","workflowStages":[]},"version":"v1","identity":"rs-1197456","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-1197456","identity":"rs-1197456","version":["v1"]},"buildId":"GqpaHPwrfC8PjnIFayRh5","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}