Sinomonas jingmaiensis sp. nov., a potentially plant growth-promoting actinobacterium isolated from the rhizosphere soil of Pu-erh tea in Jingmai Mountain

Sinomonas jingmaiensis sp. nov., a potentially plant growth-promoting actinobacterium isolated from the rhizosphere soil of Pu-erh tea in Jingmai Mountain | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Sinomonas jingmaiensis sp. nov., a potentially plant growth-promoting actinobacterium isolated from the rhizosphere soil of Pu-erh tea in Jingmai Mountain Huiqing Luo, Yan Shen, Jianyang Fu, Yuanfeng Chen, Siyu Zhou, and 7 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-7592626/v1 This work is licensed under a CC BY 4.0 License Status: Under Revision Version 1 posted 4 You are reading this latest preprint version Abstract Strain P47F4, a potentially plant growth-promoting actinobacterium isolated from the rhizosphere soil of Pu-erh tea plants in the Jingmai Pu-erh Tea District, Yunnan Province, China. The bacterium is Gram-positive, rod-shaped, and non-motile. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain P47F4 belongs to the genus Sinomonas , with its closest relative being Sinomonas humi MUSC 117 T (98.49%). The major fatty acids(> 10.0% of the total) were anteiso-C 15:0 (39.99%) and C 20:0 (10.26%). The predominant respiratory quinones detected were MK-9(H 2 ) and MK-8(H 2 ). The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, and glycolipids. Peptidoglycan analysis revealed the presence of glutamic acid, lysine, aspartic acid, serine, alanine, and glycine. The genome of strain P47F4 is 4.44 Mbp in size, with a G + C content of 66.82%. The average nucleotide identity (ANI) value of 79.70% to the most related reference strains, which is lower than the species delineation threshold of 95%. The digital DNA-DNA hybridization (dDDH) of the isolate resulted in a relatedness value of 22.90% with its closest neighbour.Based on these results, strain P47F4 represents a novel species within genus Sinomonas , for which the name Sinomonas jingmaiensis sp. nov. is proposed. The type strain is P47F4 T (= CCTCC AB 2025067 T = MCCC 1K09929 T = KCTC 59560 T ). Genome Novel species Polyphasic taxonomy Rhizosphere soil Sinomonas Full Text Additional Declarations No competing interests reported. Supplementary Files Supplementarymaterial.docx Cite Share Download PDF Status: Under Revision Version 1 posted Editorial decision: Revision requested 15 Sep, 2025 Editor assigned by journal 12 Sep, 2025 Submission checks completed at journal 12 Sep, 2025 First submitted to journal 11 Sep, 2025 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-7592626","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":515296250,"identity":"f2f48459-9f0b-493c-b8e5-0a7572794278","order_by":0,"name":"Huiqing Luo","email":"","orcid":"","institution":"Yunnan Agricultural University","correspondingAuthor":false,"prefix":"","firstName":"Huiqing","middleName":"","lastName":"Luo","suffix":""},{"id":515296251,"identity":"23d23b4c-3c45-4531-987c-854b4fd00b44","order_by":1,"name":"Yan Shen","email":"","orcid":"","institution":"Yunnan Agricultural 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Mountain","fulltext":[],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":false,"highlight":"","institution":"","isAcceptedByJournal":false,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":true,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":false,"email":"","identity":"current-microbiology","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":false,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"","title":"Current Microbiology","twitterHandle":"","acdcEnabled":false,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"VoR Journals","inReviewEnabled":false,"inReviewRevisionsEnabled":false},"keywords":"Genome, Novel species, Polyphasic taxonomy, Rhizosphere soil, Sinomonas","lastPublishedDoi":"10.21203/rs.3.rs-7592626/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-7592626/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003eStrain P47F4, a potentially plant growth-promoting actinobacterium isolated from the rhizosphere soil of Pu-erh tea plants in the Jingmai Pu-erh Tea District, Yunnan Province, China. The bacterium is Gram-positive, rod-shaped, and non-motile. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain P47F4 belongs to the genus \u003cem\u003eSinomonas\u003c/em\u003e, with its closest relative being \u003cem\u003eSinomonas humi\u003c/em\u003e MUSC 117\u003csup\u003eT\u003c/sup\u003e (98.49%). The major fatty acids(\u0026gt;\u0026thinsp;10.0% of the total) were anteiso-C\u003csub\u003e15:0\u003c/sub\u003e (39.99%) and C\u003csub\u003e20:0\u003c/sub\u003e (10.26%). The predominant respiratory quinones detected were MK-9(H\u003csub\u003e2\u003c/sub\u003e) and MK-8(H\u003csub\u003e2\u003c/sub\u003e). The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, and glycolipids. Peptidoglycan analysis revealed the presence of glutamic acid, lysine, aspartic acid, serine, alanine, and glycine. The genome of strain P47F4 is 4.44 Mbp in size, with a G\u0026thinsp;+\u0026thinsp;C content of 66.82%. The average nucleotide identity (ANI) value of 79.70% to the most related reference strains, which is lower than the species delineation threshold of 95%. The digital DNA-DNA hybridization (dDDH) of the isolate resulted in a relatedness value of 22.90% with its closest neighbour.Based on these results, strain P47F4 represents a novel species within genus \u003cem\u003eSinomonas\u003c/em\u003e, for which the name \u003cem\u003eSinomonas jingmaiensis\u003c/em\u003e sp. nov. is proposed. The type strain is P47F4\u003csup\u003eT\u003c/sup\u003e (=\u0026thinsp;CCTCC AB 2025067\u003csup\u003eT\u003c/sup\u003e\u0026thinsp;=\u0026thinsp;MCCC 1K09929\u003csup\u003eT\u003c/sup\u003e\u0026thinsp;=\u0026thinsp;KCTC 59560\u003csup\u003eT\u003c/sup\u003e).\u003c/p\u003e","manuscriptTitle":"Sinomonas jingmaiensis sp. nov., a potentially plant growth-promoting actinobacterium isolated from the rhizosphere soil of Pu-erh tea in Jingmai Mountain","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2025-10-17 14:07:34","doi":"10.21203/rs.3.rs-7592626/v1","editorialEvents":[{"type":"communityComments","content":0},{"type":"decision","content":"Revision requested","date":"2025-09-15T11:55:31+00:00","index":"","fulltext":""},{"type":"editorAssigned","content":"","date":"2025-09-12T19:20:33+00:00","index":"","fulltext":""},{"type":"checksComplete","content":"","date":"2025-09-12T13:37:32+00:00","index":"","fulltext":""},{"type":"submitted","content":"Current Microbiology","date":"2025-09-11T13:51:07+00:00","index":"","fulltext":""}],"status":"published","journal":{"display":false,"email":"","identity":"current-microbiology","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":false,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"","title":"Current Microbiology","twitterHandle":"","acdcEnabled":false,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"VoR Journals","inReviewEnabled":false,"inReviewRevisionsEnabled":false}}],"origin":"","ownerIdentity":"6adea11f-c56e-4a8e-b5a8-67ca202bd2b9","owner":[],"postedDate":"October 17th, 2025","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"in-revision","subjectAreas":[],"tags":[],"updatedAt":"2026-04-29T10:40:08+00:00","versionOfRecord":[],"versionCreatedAt":"2025-10-17 14:07:34","video":"","vorDoi":"","vorDoiUrl":"","workflowStages":[]},"version":"v1","identity":"rs-7592626","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-7592626","identity":"rs-7592626","version":["v1"]},"buildId":"8U1c8b4HqxoKbykW_rLl7","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}