Synergic effects of biochar modifier and iron nanoparticles on bioremediation of gasoil

Synergic effects of biochar modifier and iron nanoparticles on bioremediation of gasoil | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Synergic effects of biochar modifier and iron nanoparticles on bioremediation of gasoil Fatemeh Shamloo, Soheila Ebrahimi, Faramarz Rostami Charati This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-4587718/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Bioremediation of petroleum-polluted soils is deemed nowadays extremely to remedy the soils polluted by petroleum hydrocarbons. In the study synergic impacts of two amendments included biochar and iron nanoparticles were assessed to monitor the natural polluted soil by gasoil. Contaminated soil samples were collected in a polluted site in southern Iran by gasoil about 3 mg kg − 1 of soil. Soil samples were treated with 0, 1, 5 and 10% by weight of biochar and 0, 2 and 10 g kg − 1 synthetized iron nanoparticles under the incubation at 28 ± 2°C and 70% field capacity moisture for 35 days. The samples were analyzed to measure total petroleum hydrocarbon (TPH) and microbial respiration rate. According to the results, the first order kinetic model fitted well with an R 2 value of 0.934–0.98 for the soils treated with different levels of biochar and nanoparticles. A significant and positive correlation (r = 0.774, P < 0.01) derived from a polynomial equation was observed between cumulative respiration rate and change percentage of gasoil during biodegradation (ΔTPH). Increasing of biodegradation because of higher biochar is mainly related to improvement of circumstance for higher microbial activity, while inhibition effects of iron nanoparticles on decreasing microbial activities in 10 g kg − 1 is related to toxicity of nanoparticles on microbes. After 35 days of incubation, the highest ΔTPH was observed for 10% biochar and 2 g kg − 1 iron nanoparticles, as well this treatment showed that the greatest constant of degradation ( K = 0.0628) and lowest half-life (t 1/2 =11.3 days). In overall, the results showed that combined remediation strategies profoundly improve the bioremediation rate by indigenous microorganisms and further studies needs to evaluate different level of iron nanoparticles or even in combined with other remediation technologies. Total petroleum hydrocarbons microbial activities biodegradation respiration toxicity Full Text Additional Declarations No competing interests reported. Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-4587718","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":318636954,"identity":"6621f32e-ac6a-49ac-980e-32db6a2e87eb","order_by":0,"name":"Fatemeh Shamloo","email":"","orcid":"","institution":"Gorgan University of Agricultural Sciences and Natural Resources","correspondingAuthor":false,"prefix":"","firstName":"Fatemeh","middleName":"","lastName":"Shamloo","suffix":""},{"id":318636955,"identity":"11ae9824-4159-45e3-b3de-4c4ef98c48bb","order_by":1,"name":"Soheila Ebrahimi","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAABCklEQVRIie3PsWrDMBCA4QNDJkFWFxXrCQoXDF0KzltkVgjYiwMeAx3qKVmS3eBCX6EhkFnGkCwms8EdmjcQdCk0QyXNtptuherHgzD3cRKAzfYXcwEcQCCgPgEJePqneL+eIPiG8B+JSSlFpqk+95Eh3Zw/kmR/y1abQkgMopdJeVZbAu8ubSc3z0efZtgQrE68yHA23zYhKjLz70U7wToESjRxYywJOvNtzjUR030HGdeh86UJyxS54FM0yiPZS9ANB2YL1IoAlpzRuH+L+3YYPJiLVRUWazyOXmmcCI7dbxnmS6chl2bMVmtffi4eGcujnZSLwOsiLVc1k3jtuI6lv5m22Wy2/9A3erlf5x0TfNUAAAAASUVORK5CYII=","orcid":"","institution":"Gorgan University of Agricultural Sciences and Natural Resources","correspondingAuthor":true,"prefix":"","firstName":"Soheila","middleName":"","lastName":"Ebrahimi","suffix":""},{"id":318636956,"identity":"a22979c5-22c5-4622-8c35-a039bfc6c43c","order_by":2,"name":"Faramarz Rostami Charati","email":"","orcid":"","institution":"Gornbad Kavous University","correspondingAuthor":false,"prefix":"","firstName":"Faramarz","middleName":"Rostami","lastName":"Charati","suffix":""}],"badges":[],"createdAt":"2024-06-15 20:08:17","currentVersionCode":1,"declarations":"","doi":"10.21203/rs.3.rs-4587718/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-4587718/v1","draftVersion":[],"editorialEvents":[],"editorialNote":"","failedWorkflow":false,"files":[{"id":60798404,"identity":"a476bdbc-ab99-45fc-8ef5-376eaf4fa1f9","added_by":"auto","created_at":"2024-07-22 08:33:42","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":730834,"visible":true,"origin":"","legend":"","description":"","filename":"Manuscript2.pdf","url":"https://assets-eu.researchsquare.com/files/rs-4587718/v1_covered_67e3d631-007a-4e50-a28c-1e2e72f63662.pdf"}],"financialInterests":"No competing interests reported.","formattedTitle":"Synergic effects of biochar modifier and iron nanoparticles on bioremediation of gasoil","fulltext":[],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":true,"highlight":"","institution":"","isAcceptedByJournal":false,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":true,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true},"keywords":"Total petroleum hydrocarbons, microbial activities, biodegradation, respiration, toxicity","lastPublishedDoi":"10.21203/rs.3.rs-4587718/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-4587718/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003eBioremediation of petroleum-polluted soils is deemed nowadays extremely to remedy the soils polluted by petroleum hydrocarbons. In the study synergic impacts of two amendments included biochar and iron nanoparticles were assessed to monitor the natural polluted soil by gasoil. Contaminated soil samples were collected in a polluted site in southern Iran by gasoil about 3 mg kg\u003csup\u003e\u0026minus;\u0026thinsp;1\u003c/sup\u003e of soil. Soil samples were treated with 0, 1, 5 and 10% by weight of biochar and 0, 2 and 10 g kg\u003csup\u003e\u0026minus;\u0026thinsp;1\u003c/sup\u003e synthetized iron nanoparticles under the incubation at 28\u0026thinsp;\u0026plusmn;\u0026thinsp;2\u0026deg;C and 70% field capacity moisture for 35 days. The samples were analyzed to measure total petroleum hydrocarbon (TPH) and microbial respiration rate. According to the results, the first order kinetic model fitted well with an R\u003csup\u003e2\u003c/sup\u003e value of 0.934\u0026ndash;0.98 for the soils treated with different levels of biochar and nanoparticles. A significant and positive correlation (r\u0026thinsp;=\u0026thinsp;0.774, P\u0026thinsp;\u0026lt;\u0026thinsp;0.01) derived from a polynomial equation was observed between cumulative respiration rate and change percentage of gasoil during biodegradation (ΔTPH). Increasing of biodegradation because of higher biochar is mainly related to improvement of circumstance for higher microbial activity, while inhibition effects of iron nanoparticles on decreasing microbial activities in 10 g kg\u003csup\u003e\u0026minus;\u0026thinsp;1\u003c/sup\u003e is related to toxicity of nanoparticles on microbes. After 35 days of incubation, the highest ΔTPH was observed for 10% biochar and 2 g kg\u003csup\u003e\u0026minus;\u0026thinsp;1\u003c/sup\u003e iron nanoparticles, as well this treatment showed that the greatest constant of degradation (\u003cem\u003eK\u0026thinsp;=\u003c/em\u003e\u0026thinsp;0.0628) and lowest half-life (t\u003csub\u003e1/2\u003c/sub\u003e=11.3 days). In overall, the results showed that combined remediation strategies profoundly improve the bioremediation rate by indigenous microorganisms and further studies needs to evaluate different level of iron nanoparticles or even in combined with other remediation technologies.\u003c/p\u003e","manuscriptTitle":"Synergic effects of biochar modifier and iron nanoparticles on bioremediation of gasoil","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2024-07-12 04:22:14","doi":"10.21203/rs.3.rs-4587718/v1","editorialEvents":[{"type":"communityComments","content":0}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"e92b3589-cb18-40de-b97f-bbb73fc7602a","owner":[],"postedDate":"July 12th, 2024","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"posted","subjectAreas":[],"tags":[],"updatedAt":"2024-07-22T08:25:35+00:00","versionOfRecord":[],"versionCreatedAt":"2024-07-12 04:22:14","video":"","vorDoi":"","vorDoiUrl":"","workflowStages":[]},"version":"v1","identity":"rs-4587718","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-4587718","identity":"rs-4587718","version":["v1"]},"buildId":"qtupq5eGEP_6zYnWcrvyt","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}