Dataset related to "AntiCD44-gold nanoparticles for endometriosis photothermal therapy"

Dataset related to "AntiCD44-gold nanoparticles for endometriosis photothermal therapy" Authors/Creators Description Endometriosis is an inflammatory gynecological disorder that significantly impairs the quality of life of many women, presenting with debilitating symptoms. To date, conventional therapeutic approaches to manage the disease, although effective in reducing endometriotic lesions, are not entirely successful. Current pharmacological treatments often induce various side effects, leading to the elimination of both diseased and healthy cells, and recurrences are frequent. The application of nanotechnology holds significant promise for revolutionizing the diagnosis and treatment of endometriosis. Nanomedicine offers the potential to overcome these limitations, providing numerous advantages such as low toxicity and the capability to functionalize gold nanoparticles for targeted delivery to specific cell types. The objective of this study was to employ a nanotechnological approach to synthesize gold nanoparticles that are biocompatible with biological systems and selective for endometriotic cells. The ultimate goal is to enhance the efficacy and specificity of treatments for endometriosis. The nanoparticles were decorated with an antiCD44 antibody to specifically target the endometriotriotic cells of interest that overexpress the CD44 receptor. Taking advantage of these characteristics, it was possible to implement a so-called photothermal therapy, this involves the use of a laser light capable of reaching and damaging endometriotic cells while preserving the surrounding ones. The nanosystem was produced in two different shapes, sphere and star, and was characterized chemically and physically. After that, the nanoparticles were tested biologically using an endometriosis cell line that overexpresses the CD44 receptor, a healthy endometrium line with low expression of the receptor, and a line negative for the CD44 receptor. Photothermal nanosystem therapy was studied in a two-dimensional system and a 3D system. The 3D model was tuned to recreate the endometriosis environment and mimic the in vivo model. Finally, the nanosystem showed safety and efficacy in the mouse model in which endometriosis was induced. In conclusion, the proposed study demonstrated safety and efficacy of the nanosystem, particularly of star shape, in implementing photothermal therapy that can act on endometriosis cells and not affect the surrounding healthy cells. Additional details Funding - Ministero della Salute - AntiCD44-gold nanoparticles for endometriosis photothermal therapy ENDO-2021-12371975