Genomics Reveal Staphylococcus aureus Persists during Long-term Urinary Catheterization Despite Antimicrobial Therapy and Catheter Exchanges

Genomics Reveal Staphylococcus aureus Persists during Long-term Urinary Catheterization Despite Antimicrobial Therapy and Catheter Exchanges | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Article Genomics Reveal Staphylococcus aureus Persists during Long-term Urinary Catheterization Despite Antimicrobial Therapy and Catheter Exchanges Jennifer Walker, Jesus Duran Ramirez, Chelsie Armbruster, Blake Hanson This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-6702271/v1 This work is licensed under a CC BY 4.0 License Status: Published Journal Publication published 13 Jan, 2026 Read the published version in Nature Communications → Version 1 posted You are reading this latest preprint version Abstract Urinary catheters, the most frequently placed medical devices in the US, increase the risk of developing symptomatic catheter-associated urinary tract infection (CAUTI) and asymptomatic bacteriuria (ASB) – the presence of bacteria in the urine – with those requiring long-term urinary catheters (LTUCs) at highest risk. While ASB and CAUTI are caused by a broad range of uropathogens, most remain understudied. We used whole-genome sequencing to investigate the understudied uropathogen, Staphylococcus aureus . Analysis of 153 longitudinal S. aureus isolates previously collected from urinary catheter or urine samples from 20 individuals with LTUCs (average of 8 longitudinal isolates/person) demonstrated that most strains were multidrug resistant (MDR), including methicillin-resistant S. aureus (MRSA), and sequence type 5. Importantly, the same S. aureus strain persisted as ASB for an average of 13 weeks, despite antibiotic exposures or urinary catheter exchanges, and in one case transitioned to symptomatic CAUTI. The longitudinal strains were highly genetically related with few genomic changes and stable antimicrobial resistance and virulence gene carriage. This work demonstrates that MDR S. aureus can persist as ASB long-term and that common strategies to reduce or eliminate microbes from LTUCs were ineffective at eradicating the uropathogen in most cases, despite phenotypic susceptibility to the administered antibiotic. Health sciences/Urology/Urogenital diseases/Urinary tract infection Biological sciences/Microbiology/Bacteriology CAUTI asymptomatic bacteriuria Staphylococcus aureus persistence long-term human urinary catheterization Figures Figure 1 Figure 2 Figure 3 Figure 4 Figure 5 Figure 6 Introduction Urinary catheters are the most frequently placed medical devices in the United States, with > 30 million used annually 1 , 2 . Their widespread use is due to their efficacy in the clinical management of hospitalized and critically ill patients, of which 25% and 60% receive a urinary catheter, respectively 3 . Urinary catheters are also extremely effective at improving the quality of life for millions of individuals, including those with neurogenic bladder and the elderly 4 , 5 . However, urinary catheters increase the risk of developing symptomatic catheter-associated urinary tract infections (CAUTIs) – with individuals requiring long-term (> 28 days) urinary catheters (LTUCs) at highest risk 6 , 7 . Additionally, urinary catheters increase the incidence of asymptomatic bacteriuria (ASB) – the presence of bacteria in the urine without symptoms 3 . Persistent ASB, particularly among those with LTUCs, also increases the risk of developing symptomatic CAUTI 8 , 9 . Challengingly, studies demonstrate that prophylactic antibiotic treatment of individuals with urinary catheters does not eliminate ASB nor does it prevent catheter colonization, even during short-term (< 28 days) catheterization 3 , 10 – 12 . Prophylactic antibiotic treatment is, however, associated with the development of antimicrobial resistance (AMR), as well as other serious illnesses, including Clostridioides difficile colitis 13 , 14 . Additional studies indicate empiric antibiotic treatment of CAUTI also does not improve patient outcomes 15 , 16 . Thus, management of ASB and prevention and treatment of CAUTI remain difficult. Due to the high infection rates of urinary catheters (10–30%) combined with a lack of prevention strategies, CAUTIs have become the most common hospital-associated infections in the U.S., accounting for > 1 million cases and resulting in as much as $ 1.83 billion/year in associated preventable costs 17 – 19 . CAUTIs can also progress to severe disease, including bacteremia and sepsis, which increases healthcare costs, length of hospital stay, morbidity, and mortality 20 – 22 . Both CAUTI and ASB are caused by a broad range of uropathogens, including Gram-negatives such as Escherichia coli, Klebsiella pneumoniae , and Pseudomonas aeruginosa , as well as Gram-positives like Enterococcus faecalis and Staphylococcus aureus 23 , 24 . Increasing AMR among these uropathogens not only limits treatment options, but it further complicates empiric treatment 25 , 26 . Additionally, CAUTI and ASB in individuals with LTUCs are typically polymicrobial, making it difficult for physicians to differentiate uropathogens that cause symptoms from those that are asymptomatically colonizing 27 , 28 . Despite the significant challenges associated with identification and treatment of these diverse uropathogens, the majority remain understudied. Understanding how these uropathogens persist as ASB despite prophylactic antibiotic administration is critical for developing and implementing treatment strategies that reduce the disease burden associated with these common and costly infections. In this study, we focus on the understudied uropathogen S. aureus . Previous studies demonstrate that i) S. aureus within the urinary tract is primarily associated with urinary catheterization, ii) for those with persistent S. aureus ASB, one third will go on to develop a symptomatic UTI, and iii) UTI symptoms caused by S. aureus are typically more severe, with higher rates of dissemination to secondary bacteremia, compared to other uropathogens 9 , 29 – 33 . Additionally, recent studies indicate S. aureus is a common asymptomatic colonizer of LTUCs; yet there is a paucity of studies investigating the lifecycle of this pathogen in the urinary tract, particularly within LTUCs 3 , 11 . We used whole-genome sequencing to analyze a collection of S. aureus strains previously isolated from 20 individuals with LTUCs 3 , 11 . A total of 171 strains, with an average of 8 longitudinal isolates collected per person over an average collection duration of 9 months, were included in these analyses. We discovered that most individuals were colonized with methicillin resistant S. aureus (MRSA) belonging to multi-locus sequence type (ST) 5. Notably, most participants received multiple antibiotics over the course of the collection period, with nitrofurantoin and ciprofloxacin the two most commonly prescribed 3 , 11 . Antimicrobial susceptibility testing revealed all S. aureus isolates tested were susceptible to nitrofurantoin, but > 60% were resistant to ciprofloxacin. Despite anti-staphylococcal antibiotic exposure and frequent catheter exchanges, longitudinal isolates from a given participant – termed within-participant – were highly genetically related, with a median of four changes (gene gain/loss) to the accessory genomes and a median pairwise SNP distance of seven single nucleotide polymorphisms (SNPs). Virulence factor and AMR determinant carriage was also stable among within-participant isolates. Furthermore, two participants developed symptomatic S. aureus CAUTI during the collection period, of which one of the S. aureus isolates was detected as ASB for months prior to and after the symptomatic events. These results suggest that the two strategies used most frequently to try to reduce or eliminate ASB (antimicrobial administration 34 and catheter exchange 7 ) are ineffective at preventing S. aureus from persisting in individuals with LTUCs. Collectively, this study demonstrates S. aureus persistently colonizes the catheterized urinary tract, serving as a reservoir for AMR makers and subsequent symptomatic infection. Results Epidemiology of S. aureus isolates from individuals with LTUCs S. aureus strains were longitudinally isolated from individuals with LTUCs from two separate, previously published cohorts 3 , 11 , 12 . The first cohort consists of urology outpatients (UPs) from a single outpatient clinic 11 , 12 . For this cohort, microbes, including S. aureus , were cultured monthly from urine and LTUCs for approximately 12 sampling time points. The second cohort consists of nursing home residents (NHRs) from two long-term care facilities 3 . For this cohort, microbes, including S. aureus , were cultured weekly from urine samples for up to 27 sampling time points. While the sample collection type and sites of each cohort differed, the average participant age was similar, 63.4 (± 15.5 years) for the UP and 65 (±15 years) for the NHR cohorts 3 , 11 . Additionally, each cohort consisted of 10 participants that were colonized with S. aureus during at least one sampling time point (Table 1 ). For the 10 UPs, they received an average of 2.9 antibiotic treatments per participant, 50% were female, and S. aureus strains were collected on average every 27.4 days at the time of the scheduled catheter exchange. For the 10 NHRs, they received an average of 1.5 antibiotic treatments per participant, 20% were female, and S. aureus strains were collected on average every seven days – with catheter exchanges occurring every 38.12 days on average. There were no significant differences observed for the gender, antibiotic exposures, or catheter duration between the UPs and NHRs colonized with S. aureus . Overall, the demographics and epidemiology of individuals with S. aureus colonization within both cohorts were similar. Table 1 Participant demographics and epidemiology of clinical isolates from participants with S. aureus colonization of long-term urinary catheters. Statistical analyses were performed using the Fisher’s exact test denoted with * or the Mann-Whitney test denoted with # . Urology Outpatients (UP) Nursing Home Resident (NHR) p-value Participants with S. aureus 10 10 NA Female Participants 50% 20% 0.3498* Percent of Participants with MRSA Colonization 40% 60% 0.6563* Average Antibiotic Treatments per participant 2.9 1.5 0.2867# Average Catheterization Duration 27.40 days 38.12 days 0.6698# Number of S. aureus Isolates 38 133 NA Average Number of S. aureus isolates per participant 3.8 13.3 NA S. aureus LTUC isolate collection A total of 171 S. aureus isolates were collected from 273 sampling time points across these 20 participants ( Table S1 ). Most participants had a single isolate per species collected at each time point. However, several individuals had multiple S. aureus strains collected within a single time point – defined as co-isolated. These co-isolated strains were collected based on phenotypic differences, including size, color, rugosity, and antibiotic resistance, and were more frequent in the UP cohort due to culturing of both urine and catheter samples 3 , 11 . Whole-genome sequencing confirmed all 171 strains were S. aureus ( Table S1 -S3 ). Within the UPs, 38 S. aureus isolates were sequenced, averaging 3.8 strains per participant (Fig. 1 , Tables 1 and S1-S3). Within the NHRs, 133 strains were sequenced, averaging 13.3 isolates per participant (Fig. 1 , Tables 1 and S1-S3). Notably, two participants within the UP cohort experienced symptomatic S. aureus CAUTI 11 . None of the participants in the NHR cohort were diagnosed with CAUTI attributed to S. aureus 3 . While the majority of samples collected from these 20 participants were polymicrobial, S. aureus was observed to be monomicrobial at four separate time points (Fig. 1 ). Despite multiple antibiotic exposures and catheter exchanges over the course of sample collection, only two of the 273 samples collected had no detectable uropathogens across both cohorts. These results indicate that S. aureus is a common, asymptomatic colonizer that can be mono-microbial within individuals with LTUCs. Genomic analyses of S. aureus LTUC co-isolated strains To determine the population heterogeneity of co-isolated strains and investigate whether strains isolated from the urine and LTUC were genetically related, we analyzed 29 co-isolated genomes from four patients across 10 time points (Fig. 1 and Table S1 ): NHR 201 at collection 9 (n = 2; both urine), 12 (n = 2; both urine), and 14 (n = 2; both urine); UP 86 at collection 7 (n = 2; 1 catheter, 1 urine) and 10 (n = 2; both catheter); UP 111 at collection 0 (n = 2; 1 catheter, 1 urine); and UP 119 at collection 0 (n = 2; 1 catheter, 1 urine), 7 (n = 9; 6 catheter, 3 urine), 8 (n = 4; 1 catheter, 3 urine), and 10 (n = 2; 1 catheter, 1 urine). Strains isolated from the same collection time point were the same ST ( Table S2 ). Additionally, analyses of the accessory genome and SNPs ( Table S4-5 ) revealed that co-isolates displayed a median of 2.5 (IQR 1-12.5) accessory genome changes and a median pairwise SNP distance of five (IQR 3–7), regardless of culture site or colony phenotype ( Figure S1 A and S1B ). Notably, both MRSA and methicillin-susceptible S. aureus (MSSA) were co-isolated from NHR 201. In-depth analyses of genomic differences between the MRSA and MSSA co-isolates (n = 2; two collection times) revealed that despite both lineages sharing the same ST (ST5), there was a median of 72 (IQR 69–75) accessory genomes changes and a median pairwise SNP distance of 50 ( Figure S1 C and S1D ). These results suggest these co-isolates are from different lineages, despite isolation at the same collection time and sharing the same ST. Together, these findings indicate that most participants had a single, homogenous population of S. aureus with few genomic differences that predominated at each collection time, and that the strain colonizing the catheter is highly genetically related to the strain colonizing the urine. Phylogenetic analyses of longitudinal S. aureus LTUC isolates To determine whether longitudinally collected S. aureus LTUC isolates among both cohorts undergo frequent strain replacement – due to antibiotic exposure and catheter exchanges – or if they are the same strain persisting over time, a total of 153 longitudinal strains were selected for further analyses, representing a single strain from each participant at each collection time. Approximately 40% of UP and 60% of NHR strains carried mecA , classifying them as MRSA (Table 1 and Fig. 2 ). The most common lineage was ST5, which colonized 55% (11/20) of participants (Figs. 2 , S2, and S3). The second most common lineage was ST45, which colonized 15% (3/20) of participants, followed by ST8 found in 10% (2/20) of participants. Impactfully, most participants (90%; 18/20) were colonized with the same ST over time (Figs. 1 and 2 ). There were only two instances (a single participant from each group) where the initial ST detected changed to a different ST at a later collection time: NHR 101 and UP 119. Phylogenetic analyses, using the well-characterized S. aureus strain MRSA-1369 to root the tree, demonstrated that within-participant longitudinal isolates of the same ST clustered together (Figs. 2 , S2, and S3). The longitudinal isolates with different STs – from the participants that experienced the ST changes – clustered separately. Additionally, participant isolates were observed to form sub-clusters based upon mecA carriage (Figs. 2 and S3). Specifically, the longitudinal MSSA and MRSA isolates colonizing NHR 201 at alternating time points across collection times each formed distinct clusters, separate from one another. Together, these clustering patterns suggest that within-participant, longitudinal S. aureus isolates are more closely related to each other than they are to isolates from other participants, even from the same ST. Importantly, this pattern was observed across geographically distinct cohorts, suggesting these results are broadly applicable to individuals with LTUCs colonized with S. aureus . Accessory genome analyses of longitudinal S. aureus LTUC isolates To determine whether there were changes in the gene repertoire of longitudinal S. aureus isolates over time, a pan-genome analysis was performed ( Table S4 and Figure S4 ). Changes to the accessory genome were determined by comparing the initial isolate to each subsequent longitudinal isolate. For within-participant longitudinal isolates with STs that differed from the index isolate (UP 119 and NHR 101), a median of 126 (IQR 118–129) accessory genome changes were observed (Fig. 3 ). In contrast, comparisons between the first strain isolated of the same ST as the subsequent longitudinal isolates from UP 119 and NHR 101 revealed a median of 3 (IQR 1–13) accessory genome changes. Thus, the first occurrence of a new ST was determined to be an index isolate for all subsequent longitudinal isolates that were the same ST. Importantly, comparisons of all longitudinal isolates to their respective index isolate demonstrated that a median of four (IQR 1–19) accessory genome changes were observed (Fig. 3 ). Additionally, the NHR 201 index (MRSA) isolate compared to the five longitudinal MSSA strains indicate a median of 69 (IQR 67.50–71.50) genes are absent from the MSSA genomes (Fig. 3 and S5A). Most absent genes were associated with the SCC mecA Type II (2A) cassette, which is present in the MRSA isolates. Notably, comparing the first MSSA strain isolated (NHR 201-6) to the longitudinal MSSA strains, a median of 3 (IQR 1-6.5) accessory genome changes were observed ( Figure S5B ). Together, these findings indicate that there are few changes to the accessory genomes of longitudinal isolates with the same ST and mecA carriage over time. SNP analyses of longitudinal S. aureus LTUC isolates To determine the genomic diversity of longitudinal S. aureus isolates over time, SNP analyses were performed (Fig. 4 and Table S5 ). A median pairwise SNP distance of 2,344 (IQR 1178–38777) was observed for all the index (first isolate of a unique ST) isolates between participants. For all isolates between participants with the same ST, the median pairwise SNP difference dropped to 1,120 (IQR 614–1767), demonstrating that isolates from the same ST are more closely related to each other than to other STs (p-values < 0.0001), as expected 35 . Notably, within-participant longitudinal isolates, including those with ST changes, displayed a median pairwise SNP difference of eight (IQR 3–25). Furthermore, among within-participant longitudinal isolates of the same ST, the median pairwise SNP difference dropped to seven (IQR 2–13). Additional analyses of the longitudinal MRSA and MSSA populations from NHR 201 revealed the MSSA strains isolated across all five collection times differed by a median of 25 (IQR 21.75–29.75) SNPs ( Figure S5C ), while the median pairwise SNP difference among the MRSA strains isolated across the other 10 collection times was 67 (IQR 25.5–75.5) ( Figure S5D ). Together, these data demonstrate that within-participant longitudinally collected isolates are a highly genetically related, single lineage that persists over time despite repeated catheter exchanges and anti-staphylococcal antibiotic exposures. Antimicrobial susceptibility profiles of S. aureus LTUC isolates Most participants (12/20) received at least one antibiotic during the collection period (Fig. 1 ), and all isolates carried genes conferring resistance to multiple antibiotics (Fig. 5 and Table S6 ). Specifically, all isolates carried determinants that confer resistance to tetracyclines and Fosfomycin, and most encoded AMR genes associated with resistance to quinolones, macrolides, beta-lactams, and aminoglycosides. Only a few strains encoded AMR genes that confer resistance to lincosamide, rifamycin, mupirocin, bleomycin, and streptothricin. Importantly, the AMR determinant profiles were mostly stable among within-participant longitudinally collected isolates. Among the few isolates where AMR determinant instability was observed, these genes are associated with aminoglycoside resistance, which are generally carried on mobile genetic elements that are known to be readily gained and lost due to selective pressures. Additionally, a single instance of the loss of mecA- associated genes was observed in an isolate from NHR 104 (104 − 21). SNP analysis revealed that the previously persisting MRSA strain and the subsequent MSSA strain differed by only one SNP (a missense mutation in a hypothetical protein), indicating this strain lost the mecA cassette ( Table S6 ). Next, to determine the antimicrobial susceptibilities to the most commonly prescribed antibiotics for UTI, as well as some of the anti-staphylococcal antibiotics these participants received, minimum inhibitory concentration (MIC) assays were determined for index and final longitudinal isolates (Tables 2 and S7). Additionally, NHR 104 − 21 was included due to notable changes in its AMR determinant profile, specifically the loss of mecA when compared to the index and other longitudinal isolates from NHR 104 (Fig. 5 ). NHR 201 − 11 was also selected based on similar changes; mecA was absent in the final isolate (NHR 201 − 17) compared to the index isolate (NHR 201-0), and NHR 201 − 11 was the last longitudinal isolate encoding mecA . Antibiotics assessed included nitrofurantoin, trimethoprim-sulfamethoxazole, ciprofloxacin, oxacillin, penicillin, and vancomycin. All isolates assessed were susceptible to nitrofurantoin, trimethoprim-sulfamethoxazole, and vancomycin. Most index and final isolates (10/13) retained the same antibiotic susceptibility pattern. Phenotypic non-susceptibility to oxacillin was generally concordant with genotype, with strains encoding mecA displaying resistance to oxacillin, and those that did not, including those that lost mecA (e.g. NHR 104 − 21), displaying susceptibility. However, both strains isolated from UP 129 encoded mecA , yet the strains were phenotypically susceptible to oxacillin and are therefore classified as MSSA. A missense mutation in mecA (T547C) was observed in both isolates, potentially explaining the susceptibility. Furthermore, in contrast to the index isolate, UP 129’s final isolate was susceptible to penicillin, which may be due to an additional missense mutation (C299T) in blaZ . Together, these results demonstrate that there was concordance between genotypic and phenotypic antimicrobial susceptibility patterns of persistent S. aureus LTUC isolates, which remained largely stable despite exposures to antimicrobials. Carriage of AMR determinants and virulence factors is negatively correlated To determine the virulence factor carriage profile of S. aureus LTUC isolates, the virulence determinants encoded by all isolates were identified ( Figure S6 and Table S8 ). Similar to the AMR determinants, virulence gene profiles remained stable for most longitudinal isolates. Additional correlation analyses of the index isolates were used to determine whether there is a relationship between the number AMR determinants and virulence genes encoded by uropathogenic S. aureus . A significant negative correlation was observed for the number of AMR determinants and virulence genes encoded (Fig. 6 ). Together, these data indicate that uropathogenic S. aureus strains that carry more AMR genes encoded fewer virulence factors. Discussion Urinary catheterization is a common procedure that improves the quality of life for individuals with urinary tract dysfunction 4 , 5 . While this procedure is beneficial, urinary catheter duration is correlated with the risk of developing ASB and CAUTI, with longer dwell times associated with the highest risk 3 , 6 – 8 , 36 . Current treatment and prevention strategies include antimicrobial therapy and catheter exchanges 7 , 34 . Yet, recent studies demonstrate that prophylactic antibiotic administration does not prevent catheter colonization – even during short catheter durations – and may lead to more severe diseases, include C. difficile colitis, as well as the selection of AMR 3 , 11 – 14 . Additionally, CAUTIs continue to be one of the most common hospital-associated infections in the US 28 , 37 , 38 . Previous studies indicate that the presence of S. aureus in the urinary tract i) is predominately associated with a urinary catheter, ii) is mainly MRSA, iii) can increase reflux to the kidney by blocking catheters via urease-produced encrustations, and iv) has a higher risk of disseminating to bacteremia compared to other uropathogens 9 , 12 , 29 – 33 , 39 . S. aureus is also a frequent cause of ASB, and persistent S. aureus ASB is associated with development of symptomatic infection 3 , 11 , 30 . Yet, S. aureus remains largely uncharacterized during its lifecycle in the catheterized urinary tract. This study analyzed longitudinally collected S. aureus strains isolated from two distinct cohorts: UPs and NHRs 3 , 11 . The demographics of both cohorts, including age, urinary catheter duration, and exposure to antibiotics, were similar. Whole-genome sequencing of all S. aureus strains revealed ST5 as the most frequently isolated lineage among both cohorts. However, the second most common ST differed between cohorts: ST8 and ST45 among UPs and NHRs, respectively. ST5, ST8, and ST45 are among the most frequently circulating lineages causing infection within the US 40 – 43 . Additionally, recent studies indicate ST5 is a common lineage found within the urinary tract globally 44 , 45 . While most mechanistic studies of S. aureus in the urinary tract assess ST8 isolates 29 , 45 , 46 , ST5 remains largely uncharacterized during CAUTI or ASB. Notably, the UP cohort was colonized with a wider diversity of STs compared to NHRs (eight vs four STs, respectively). This ST diversity may be due to the environmental differences within which UPs and NHRs reside 43 , 47 , 48 . NHRs live together in nursing homes, which may serve as a common environmental source of pathogen sharing. In contrast, UPs primarily reside at home within the community, resulting in potentially more diverse and participant-specific environmental sources of pathogen sharing. S. aureus was present at the first collection time point for 65% (13/20) of participants across both cohorts. Importantly, among within-participant longitudinal isolates, the same ST was stably detected over time despite regular catheter changes (~ 27 days for UPs and ~ 38 days for NHRs). There were only two instances – one participant in each cohort (NHR 101 and UP 119) – where the index isolate ST changed to a different ST at subsequent collections. NHR 101 was initially colonized with ST5; however, after a catheter exchange and trimethoprim-sulfamethoxazole administration, S. aureus was no longer detected in the urine over the next 10 collections (10 weeks). At collection 19, an ST45 S. aureus was cultured from the urine. Similarly, the index isolate collected for UP 119 was ST59. S. aureus was not cultured from catheters over the next four collections (16 weeks), however, after ciprofloxacin treatment during collection four and five, an ST30 S. aureus was cultured from the catheter at collection six. This ST30 lineage persisted despite regular, monthly catheter exchanges until the end of the study period. Notably, among the 35% (7/20) of participants that acquired S. aureus during the collection period, all but one participant (NHR 204) received a catheter exchange or antibiotic exposure immediately prior to S. aureus isolation. Together, these data suggest that catheter exchanges and antibiotic exposures may disrupt communities, eliminating other uropathogens, leaving a niche open for S. aureus to rapidly recolonize. Additionally, while trimethoprim-sulfamethoxazole may have some efficacy at temporarily reducing S. aureus bacterial burdens in the catheterized urinary tract, this contrasts with other anti-staphylococcal antibiotics, such as nitrofurantoin 3 , 11 , 49 , which had no effect on the persistence of UP 86’s S. aureus isolates despite phenotypic susceptibility in vitro . Phylogenetic analyses of longitudinal S. aureus strains demonstrate that within-participant isolates cluster more closely together compared to isolates from between participants, even among ST groups. Further genomic analyses indicated that within-participant isolates with the same ST were highly genetically related, with a median of four accessory genome changes and a median pairwise SNP difference of seven. For example, the 16 isolates from NHR 207 had a median of two accessory genome changes and a median pairwise SNP difference of seven. Additionally, among the four participants with co-isolates, genomic analyses demonstrated that these strains displayed a median of 2.5 accessory genome changes and a median pairwise SNP difference of five. Notably, while the MRSA and MSSA co-isolates from NHR 201 were from different lineages (72 accessory genome changes and SNP distance of 50) and the longitudinal MRSA strains were more heterogeneous (five accessory genome changes and SNP distance of 67), the longitudinal MSSA isolates were highly related to each other (three accessory genome changes and SNP distance of 25). NHR 201 had multiple antibiotic exposures over the course of the collection time, which may have provided additional pressures enabling more diverse lineage colonization. Interestingly, the MRSA strain colonized first, with the MSSA strain subsequently invading and eventually replacing the MRSA lineage. Taken together, these results are consistent with a previous study indicating a difference of 20 or fewer SNPs between strains can be used to identify a single, persisting S. aureus strain 50 . Additionally, these data demonstrate that S. aureus stably and asymptomatically persists within the catheterized bladder for up to nine months, resulting in persistent ASB. Importantly, most participants are dominated by a single homogeneous population that is highly genetically related, indicating a single lineage persists over time. This pattern was observed across geographically distinct sites (Midwest vs Northeast), suggesting these results are broadly applicable to individuals with LTUCs colonized with S. aureus . Impactfully, the observed SNP increases are greater than the expected average accumulation of 2.72 SNPs per megabase per year observed when S. aureus stably colonizes the nares 51 , indicating S. aureus may be adapting to the urinary tract or exposure to antibiotics over time. Consistent with previous reports 9 , 30 , half of the S. aureus LTUC strains carried mecA and were resistant to oxacillin, making them MRSA. Additionally, most LTUC strains carry genes that confer resistance to a broad array of antibiotic classes, including fosfomycin and tetracyclines. Importantly, AMR gene carriage was stable across longitudinal isolates, despite most participants receiving at least one antibiotic during the study period and many receiving anti-staphylococcal antibiotics, including ciprofloxacin (5/20), trimethoprim-sulfamethoxazole (3/20), nitrofurantoin (3/20), and cephalexin (2/20) 3,11 . MIC assays using the antibiotics participants received or those that are commonly used to treat CAUTI demonstrated that antibiotic susceptibility patterns were stable among longitudinal isolates, with most strains (both index and final) displaying resistance to ciprofloxacin but susceptibility to nitrofurantoin. Despite treatment with antibiotics to which these isolates should be susceptible, the same strain was found to persist over the course of 12 months. For example, UP 86 received ciprofloxacin and nitrofurantoin over the course of the collection period 11 and the index and final isolates (collected 12 months apart) were intermediate resistant and susceptible, respectively. Of the few strains that exhibited discordant antimicrobial susceptibility patterns between the index and final isolates, the most notable were the NHR 201 MRSA and MSSA isolates. However, the NHR 201 index and final MRSA isolates and the final MSSA isolate exhibited concordant genotypic and phenotypic beta-lactam susceptibility, with the differences due to invasion and replacement by a different lineage. Additionally, MRSA stably colonized NHR 104 for 26 weeks, yet mecA was lost at a single collection time (21), making it a MSSA strain. Since loss of mecA is rare, additional analyses performed determined that the MSSA and MRSA strains were the same ST and the strains collected at time point 0 (MRSA) and 21 (MSSA) differed by only one SNP, which occurred in a hypothetical protein. These results demonstrate that the MSSA and MRSA are the same strain, and that, while rare, mecA , and therefore resistance to beta-lactams, was lost during persistent S. aureus ASB. In contrast to the index isolate, UP 129’s final isolate was also susceptible to penicillin likely due to a missense mutation (C299T) in blaZ , a gene that encodes a beta-lactamase. MIC assays with oxacillin confirmed the predicted genotypic susceptibility of longitudinal isolates was generally concordant with phenotypic susceptibility. The only exceptions to this concordance were isolates from UP 129, which encoded the Scc mec Type IVc(2B), yet were phenotypically susceptible to oxacillin and therefore MSSA. A missense mutation in mecA (T547C) was observed in both isolates, potentially explaining the susceptibility. Together, these results highlight that LTUC isolates are multidrug resistant, further strengthening the hypothesis that ASB serves as a reservoir for drug resistant S. aureus . In addition to AMR genes, virulence factor carriage was also stable among longitudinal isolates. Impactfully, carriage of AMR genes was inversely correlated with the presence of virulence factors: strains with increased AMR determinants encoded fewer virulence factors. Notably, the stable carriage of AMR genes across longitudinal S. aureus LTUC isolates may help guide the selection of empiric antibiotics for treatment of subsequent CAUTI and therefore improve patient outcomes. Challengingly, these data also suggest that phenotypic resistance does not always correlate with S. aureus persistence, as some strains that were susceptible to the antibiotics participants received during the collection period persisted as ASB. Thus, studies are needed to further dissect the S. aureus recalcitrance mechanism that promote persistence. This study was limited by the scope of both cohorts, which prioritized the isolation and sequencing of a single isolate per species for each participant at each collection. This collection strategy may limit the detection of heterogenous populations that may be present, as shown with NHR 201. Additionally, only the NHR cohort was designed to differentiate symptomatic CAUTI from ASB, which limited our ability to determine if strains persisting as ASB transitioned to cause symptomatic CAUTI in our UP cohort. Finally, while we used both long-and short-read sequencing for index isolates, only short-read sequencing was performed for longitudinal- and co-isolates. This approach allowed us to define the genomic synteny (long reads) and polish the genomes (short reads) of the index isolates to assess SNPs over time. However, without long-reads for the longitudinal and co-isolates, we may have missed large scale structural variations or gene gain/loss events that resulted from the movement or acquisition of mobile genetic elements. Notably, however, we were able to identify longitudinal and co-isolates with large accessory genome changes, limiting this concern. In depth analyses of these observations indicated two likely causes: 1) ST switches within participants suggest these changes were due to strain lineage differences, and 2) loss of large mobile genetic elements, including the mecA cassette, with few or no SNPs differences, indicate these are the same strain with gene loss. Nevertheless, most longitudinal and co-isolates were highly genetically related, and the pan-genome repertoire of each longitudinal set of isolates was stable, suggesting there are minimal longitudinal structural differences within most of the strain collection. Thus, most strains stably persist as ASB during long-term catheterization. Our study demonstrates that a single S. aureus strain persists over time despite antibiotic exposure or catheter exchanges, which are the two accepted practices for reducing microbial burdens during catheterization 7 , 34 . Importantly, these results are broadly applicable to LTUCs colonized with S. aureus , as this pattern was observed across geographically distinct sites. Furthermore, while all strains tested are susceptible to nitrofurantoin, most strains (> 61%) are resistant to ciprofloxacin, which are two commonly prescribed antibiotics for the treatment of symptomatic CAUTI 7 , 11 , 28 , 52 . The ability of S. aureus to persist despite susceptibility to the antibiotics participants received during the collection period warrants further investigation into the mechanisms driving this phenotypic recalcitrance. Importantly, the stable carriage of AMR determinants among longitudinal isolates suggests that monitoring AMR patterns of persistent S. aureus ASB isolates may help guide physician selection of antibiotics to treat subsequent symptomatic CAUTI, which may improve the efficacy of empiric therapy and therefore patient outcomes. Finally, the accumulation of SNPs within longitudinal isolates suggest uropathogenic S. aureus LTUC isolates are under selection. Future studies are needed to determine if and how S. aureus is adapting to selective pressures, such as the urinary tract or antibiotics. Methods Strains and growth conditions. All strains used in this study are from individuals with LTUCs from two separate, previously published cohorts and are listed in ( Table S1 ) 3 , 11 , 12 . The first cohort is comprised of individuals seen at the Division of Urology at Washington University School of Medicine in St. Louis in Missouri 11 , 12 . Isolates were collected from rich media based on unique colony morphology and 16S sequencing confirmed the species. A total of 38 S. aureus strains were collected from 2018–2021, deidentified, and used as a part of this study. The second cohort is comprised of individuals residing in two separate nursing home facilities in New York 3 . Isolates were collected from selective media and differentiated by colony morphology with additional testing performed to identify species. A total of 133 S. aureus strains were collected from 2019–2020, deidentified, and used in this study. Mannitol Salt Agar (BD, cat 211407) plates were used to confirm and isolate all S. aureus clinical isolates from both cohorts prior to whole-genome sequencing. Brain-heart infusion (BHI) broth (BD, cat. 237200) and agar (BD, cat. 214010) were used to maintain strains. Tryptic Soy Broth (TSB) (Sigma, cat. #22092-500G) was used to grow isolates for whole-genome sequencing. For MIC assays, bacterial isolates were grown in Mueller-Hinton broth (MHB) (Sigma; cat. 7019-100G) and MHB supplemented with 2% NaCl for oxacillin, according to the Clinical Laboratory Standard Institute (CLSI) 53 . Methicillin resistance using cefoxitin was previously reported for the NHR cohort 3 . Oxacillin resistance was confirmed for index and final isolates as described below. Whole-genome sequencing of the S. aureus isolates. Whole-genome sequencing of all isolates ( Table S1 ) was performed as previously described 54 . Briefly, S. aureus strains were grown in TSB for 2–4 h, shaking at 37°C. The cultures were pelleted and genomic DNA was extracted using the Qiagen QIAamp DNA minikit (Qiagen, cat. 51306) following the manufacturer's protocol, with the exception that 1 µg/mL of lysostaphin and 20 µg/mL of lysozyme was added to the initial buffer. Genomic DNA then underwent library preparation for short-read sequencing using the Illumina NextSeq 2000 sequencer. Genomes from short reads were assembled as previously described 54 . Briefly, read quality assessment was conducted with Raspberry v0.3, trimming was conducted with Trimmomatic v0.39, SPAdes v3.15.2 was used for assembly, and assembly quality was assessed using Quast v5.2.0 54 . Additional assembly quality control measures include BUSCO v5.7.0, for genome completeness, and CheckM v1.1.2, for genome quality control ( Tables S2-3 ) 55 , 56 . Isolates were re-sequenced if the genome had > 500 contigs, had a genome size greater or less than 2 standard deviations from the average of all S. aureus genomes within NCBI, or if the assembly was contaminated with another bacterial species as defined by the presence of another bacterial species identified at greater than 2% by StrainSeeker v1.5 57 . Additionally, a subset of isolates was selected for Oxford Nanopore long-read sequencing to accurately reconstruct genome structures, including the circularization of plasmids and resolution of mobile genetic element integration. Isolates were chosen based on the identified ST from short-read sequencing, with the first isolate with a unique ST within a participant’s longitudinal set selected for long-read sequencing, which we define as an index isolate ( Table S1 ). Briefly, the Oxford SQK-RBK004 library preparation kit was used to prepare bacterial DNA for sequencing on the Oxford Nanopore GridION X5 (Oxford, UK) using FLO-MIN106 flow cells. Genomes were assembled with Flye v2.9.1 and then underwent error correction and polishing steps using Racon v1.5.0 and Medaka to generate hybrid assemblies as previously described 58 . Molecular epidemiology of the bacterial assemblies MLST v2.19.0 was used to identify the ST for each isolate as previously described 54 , 59 . Gene annotations for each assembled genome were performed via Prokka v1.14.5 as previously described 54 , 60 . Pan-genome and phylogenetic analysis of the S. aureus isolates The core genome for all isolates in the dataset, including index, co-isolates, and each set of longitudinal isolates for a participant, was identified with Roary v3.13.0 on the annotated short-read assemblies 61 . Accessory gene lists obtained from the Roary output were defined as genes present in > 99% of isolates. Additionally, to quantify the gene gain/loss events for each set of longitudinal and co-isolates from a participant, the accessory genome changes were identified by comparing the gene content of the index isolate to the longitudinal or co-isolated strains. Participants with single isolates were not included as no changes in gene content could be quantified. Final analysis and visualization were made in GraphPad Prism version 10.1.1. The core-gene alignment file from Roary was used with RAxML v8.2.12 to produce maximum-likelihood phylogenetic trees that were visualized using iTOL and edited on Affinity Designer as previously described 62 – 64 . SNPs analyses of clinical isolates SNP analyses were conducted using Snippy v4.6.0, aligning the paired-end reads from the queried isolate to the index isolate ( Table S9 ) 65 . For co-isolated strains, short-read assembled genomes were used to compare the index sequence to the sequences of the other co-isolated strains. To reduce potential bias in our longitudinal analyses, co-isolated strains of the same ST and mecA carriage as the index isolate were excluded as they had an average of less than 5 SNPs. In instances where two or more isolates were collected during the index collection, catheter isolates were prioritized. To identify genomic differences in our longitudinal cohort, the hybrid assembly of the index isolate from each set, defined as the first isolate of an ST within a participant, was used as a reference for each longitudinal strain to enable comprehensive assessment of whole-genome SNPs, including those in intergenic regions, and resolve the genomic synteny of these mutations. Self-self comparisons (i.e. comparing the index isolate’s short reads against the hybrid assembly) were excluded from statistical assessments as short-reads were used to polish the long-read assemblies to remove errors. Final analysis and visualization were made in GraphPad Prism version 10.1.1. Antimicrobial resistance and virulence factor profiles The AMR profile of each strain was assessed using NCBI AMR Gene Finder Plus (AMRFinderPlus v3.11.8) for both mutational and acquired AMR mechanisms as well as species-specific virulence determinants ( Tables S5-7 ), as previously described 66 , 67 . Virulence factors were obtained using Abricate ( https://github.com/tseemann/abricate ) using the virulence factor database (VFDB), as previously described 58 , 68 . This data set was prioritized for analyses as it encompassed a larger repertoire of determinants compared to AMRFinder Plus virulence determinants ( Table S8 ). Additionally, the mecA cassette type was identified using the Scc mec Finder 1.2 webtool 69 . The number of AMR determinants identified within the index isolate was compared to the number of virulence genes and a nonparametric Spearman’s correlation analysis was used to determine statistical correlation. Longitudinal isolates were excluded as most AMR and virulence profiles remained consistent, thus including them would violate the assumption of independence required for the statistical test. Minimum inhibitory concentration assays MICs were determined based on the 2024 CLSI guidelines as previously described 58 . Briefly, isolates were grown to ~ 2.8E8 colony forming units (CFU)/mL in MHB or MHB supplemented with 2% NaCl for oxacillin per the CLSI guidelines, corresponding to an optical density at 600nm (OD 600 ) of 0.4 using a Synergy H1 Biotek microtiter plate reader 53 . Cultures were then diluted to ~ 1E6 CFU/mL and equal volumes of antibiotic and bacteria were transferred to a 96-well plate with final concentrations corresponding to the sensitive or resistant MIC breakpoints of each antibiotic tested and ~ 5E5 CFU/mL. The final antibiotic concentrations used were as follows: 1, 2, and 4 µg/ml ciprofloxacin, 32 and 128 µg/ml nitrofurantoin, 2 and 4 µg/ml oxacillin, 0.125 and 0.25 µg/ml penicillin, 2/38 and 4/76 µg/ml trimethoprim-sulfamethoxine, and 1 and 16 µg/ml vancomycin. Plates were incubated at 37° or 35°C overnight following CLSI incubation times. Resistance was determined based on an OD 600 of greater than 0.1 and each condition consisted of at least 4 replicates – 2 technical replicates and 2 biological replicates ( Table S9 ). Statistical Analysis Statistical significance between epidemiological descriptions was determined using the Fisher’s exact and the Mann-Whitney test. Significant differences for SNPs comparisons were determined using the Mann-Whitney U test. The Pearson’s correlation analysis was used to determine the correlation between number of antimicrobial resistance and virulence factors. All statistical tests were performed using GraphPad Prism version 10.1.1. Data Availability Annotated genomes were deposited into NCBI and are available under the BioProject ID: PRJNA1265249. Declarations Running Title S. aureus Persists during Urinary Catheterization Competing Interests The authors declare no competing interests. Author Contributions J.M.DR, C.E.A., B.M.H., and J.N.W. designed the research studies, J.M.DR, B.M.H., and J.N.W. performed the experiments, sequencing, data analyses, and drafted the manuscript, and all authors contributed to the editing of the manuscript. Acknowledgements This work was supported by an NIH K01 DK128381-01A1 (J.N.W.), the University of Texas STAR award (JNW), an NIH K01 AI148593-01 (BMH), and an American Heart Association Predoctoral Fellowship (JMDR). We also thank Dr. Shelby R. Simar for scientific advice and guidance. Finally, we thank the study participants for their sample donations. References Trautner BW, Darouiche RO (2004) Catheter-associated infections: pathogenesis affects prevention. Arch Intern Med 164:842–850. 10.1001/archinte.164.8.842 Flores-Mireles A, Hreha TN, Hunstad DA, Pathophysiology (2019) Treatment, and Prevention of Catheter-Associated Urinary Tract Infection. Top Spinal Cord Inj Rehabil 25:228–240. 10.1310/sci2503-228 Armbruster CE, Brauer AL, Humby MS, Shao J, Chakraborty S (2021) Prospective assessment of catheter-associated bacteriuria clinical presentation, epidemiology, and colonization dynamics in nursing home residents. JCI Insight 6. 10.1172/jci.insight.144775 Fumincelli L et al (2017) Quality of Life of Intermittent Urinary Catheterization Users and Their Caregivers: A Scoping Review. Worldviews Evid Based Nurs 14:324–333. 10.1111/wvn.12231 Pilloni S, Krhut J, Mair D, Madersbacher H, Kessler TM (2005) Intermittent catheterisation in older people: a valuable alternative to an indwelling catheter? Age Ageing 34:57–60. 10.1093/ageing/afh233 Nicolle LE (2014) Catheter associated urinary tract infections. Antimicrob Resist Infect Control 3:23. 10.1186/2047-2994-3-23 Werneburg GT (2022) Catheter-Associated Urinary Tract Infections: Current Challenges and Future Prospects. Res Rep Urol 14:109–133. 10.2147/rru.S273663 Chenoweth C, Saint S (2013) Preventing catheter-associated urinary tract infections in the intensive care unit. Crit Care Clin 29:19–32. 10.1016/j.ccc.2012.10.005 Shigemura K et al (2013) Clinical factors associated with shock in bacteremic UTI. Int Urol Nephrol 45:653–657. 10.1007/s11255-013-0449-4 Maffucci F, Chang C, Simhan J, Cohn JA (2023) Is There Any Benefit to the Use of Antibiotics with Indwelling Catheters after Urologic Surgery in Adults. Antibiot (Basel) 12. 10.3390/antibiotics12010156 Nye TM et al (2024) Microbial co-occurrences on catheters from long-term catheterized patients. Nat Commun 15:61. 10.1038/s41467-023-44095-0 Walker JN et al (2020) High-resolution imaging reveals microbial biofilms on patient urinary catheters despite antibiotic administration. World J Urol 38:2237–2245. 10.1007/s00345-019-03027-8 Cortes-Penfield NW, Trautner BW, Jump RLP (2017) Urinary Tract Infection and Asymptomatic Bacteriuria in Older Adults. Infect Dis Clin North Am 31:673–688. 10.1016/j.idc.2017.07.002 Cope M et al (2009) Inappropriate treatment of catheter-associated asymptomatic bacteriuria in a tertiary care hospital. Clin Infect Dis 48:1182–1188. 10.1086/597403 Liu F et al (2023) Prevalence and treatment of asymptomatic bacteriuria at academic and critical-access hospitals-Opportunities for stewardship efforts. Infect Control Hosp Epidemiol 44:979–981. 10.1017/ice.2022.143 Babich T et al (2017) Empirical Antibiotic Treatment Does Not Improve Outcomes in Catheter-Associated Urinary Tract Infection: Prospective Cohort Study. Clin Infect Dis 65:1799–1805. 10.1093/cid/cix680 Klein RD, Hultgren SJ (2020) Urinary tract infections: microbial pathogenesis, host-pathogen interactions and new treatment strategies. Nat Rev Microbiol 18:211–226. 10.1038/s41579-020-0324-0 Flores-Mireles AL, Walker JN, Caparon M, Hultgren SJ (2015) Urinary tract infections: epidemiology, mechanisms of infection and treatment options. Nat Rev Microbiol 13:269–284. 10.1038/nrmicro3432 Gupta P et al (2023) Reducing catheter-associated urinary tract infections in the cardiac intensive care unit with a coordinated strategy and nursing staff empowerment. BMJ Open Qual 12. 10.1136/bmjoq-2022-002214 Saint S (2000) Clinical and economic consequences of nosocomial catheter-related bacteriuria. Am J Infect Control 28:68–75. 10.1016/s0196-6553(00)90015-4 Papanikolopoulou A et al (2022) Catheter-Associated Urinary Tract Infections, Bacteremia, and Infection Control Interventions in a Hospital: A Six-Year Time-Series Study. J Clin Med 11. 10.3390/jcm11185418 Grillo S et al (2020) Characteristics and Outcomes of Staphylococcus aureus Bloodstream Infection Originating From the Urinary Tract: A Multicenter Cohort Study. Open Forum Infect Dis 7:ofaa216. 10.1093/ofid/ofaa216 Gaston JR, Johnson AO, Bair KL, White AN, Armbruster CE (2021) Polymicrobial interactions in the urinary tract: is the enemy of my enemy my friend? Infect Immun. 10.1128/iai.00652-20 Kline KA, Lewis AL, Gram-Positive Uropathogens (2016) Polymicrobial Urinary Tract Infection, and the Emerging Microbiota of the Urinary Tract. Microbiol Spectr 4. 10.1128/microbiolspec.UTI-0012-2012 Ku JH et al (2024) Antibiotic Resistance of Urinary Tract Infection Recurrences in a Large Integrated US Healthcare System. J Infect Dis 230:e1344–e1354. 10.1093/infdis/jiae233 Miller LG, Tang AW (2004) Treatment of uncomplicated urinary tract infections in an era of increasing antimicrobial resistance. Mayo Clin Proc 79:1048–1053 quiz 1053 – 1044. 10.4065/79.8.1048 Köves B, Magyar A, Tenke P (2017) Spectrum and antibiotic resistance of catheter-associated urinary tract infections. GMS Infect Dis 5(Doc06). 10.3205/id000032 Hooton TM et al (2010) Diagnosis, prevention, and treatment of catheter-associated urinary tract infection in adults: 2009 International Clinical Practice Guidelines from the Infectious Diseases Society of America. Clin Infect Dis 50:625–663. 10.1086/650482 Walker JN et al (2017) Catheterization alters bladder ecology to potentiate Staphylococcus aureus infection of the urinary tract. Proc Natl Acad Sci U S A 114:E8721–e8730. 10.1073/pnas.1707572114 Muder RR et al (2006) Isolation of Staphylococcus aureus from the urinary tract: association of isolation with symptomatic urinary tract infection and subsequent staphylococcal bacteremia. Clin Infect Dis 42:46–50. 10.1086/498518 Kulbay A, Joelsson-Alm E, Amilon K, Tammelin A (2024) Asymptomatic bacteriuria and urinary tract infection in geriatric inpatients after indwelling urinary catheter removal: a descriptive two-centre study. Infect Prev Pract 6:100411. 10.1016/j.infpip.2024.100411 Saidel-Odes L, Riesenberg K, Schlaeffer F, Borer A (2009) Epidemiological and clinical characteristics of methicillin sensitive Staphylococcus aureus (MSSA) bacteriuria. J Infect 58:119–122. 10.1016/j.jinf.2008.11.014 Huggan PJ, Murdoch DR, Gallagher K, Chambers ST (2008) Concomitant Staphylococcus aureus bacteriuria is associated with poor clinical outcome in adults with S. aureus bacteraemia. J Hosp Infect 69:345–349. 10.1016/j.jhin.2008.04.027 Westgeest AC et al (2024) Catheter replacement in catheter-associated urinary tract infection: current state of evidence. Eur J Clin Microbiol Infect Dis 43:1631–1637. 10.1007/s10096-024-04878-9 Raghuram V et al (2023) Comparison of genomic diversity between single and pooled Staphylococcus aureus colonies isolated from human colonisation cultures. bioRxiv. 10.1101/2023.06.14.544959 Moon C, Wi YM (2025) Impact of antibiotic treatment and predictors for subsequent infections in multidrug-resistant Pseudomonas aeruginosa catheter-associated asymptomatic bacteriuria. Am J Infect Control. 10.1016/j.ajic.2025.01.015 Chenoweth CE, Gould CV, Saint S (2014) Diagnosis, management, and prevention of catheter-associated urinary tract infections. Infect Dis Clin North Am 28:105–119. 10.1016/j.idc.2013.09.002 Trautner BW (2010) Management of catheter-associated urinary tract infection. Curr Opin Infect Dis 23:76–82. 10.1097/QCO.0b013e328334dda8 Stickler DJ (2014) Clinical complications of urinary catheters caused by crystalline biofilms: something needs to be done. J Intern Med 276:120–129. 10.1111/joim.12220 Loftus RW et al (2024) Characterizing the molecular epidemiology of anaesthesia work area transmission of Staphylococcus aureus sequence type 5. J Hosp Infect 143:186–194. 10.1016/j.jhin.2023.07.003 Hofstetter KS et al (2024) Strain Differences in Bloodstream and Skin Infection: Methicillin-Resistant Staphylococcus aureus Isolated in 2018–2021 in a Single Health System. Open Forum Infect Dis 11:ofae261. 10.1093/ofid/ofae261 Huang YC et al (2024) Dissemination of meticillin-resistant Staphylococcus aureus sequence type 8 (USA300) in Taiwan. J Hosp Infect 149:108–118. 10.1016/j.jhin.2024.04.024 Turner NA et al (2019) Methicillin-resistant Staphylococcus aureus: an overview of basic and clinical research. Nat Rev Microbiol 17:203–218. 10.1038/s41579-018-0147-4 Xu K et al (2023) Staphylococcus aureus ST1 promotes persistent urinary tract infection by highly expressing the urease. Front Microbiol 14:1101754. 10.3389/fmicb.2023.1101754 Saenkham-Huntsinger P et al (2021) Copper Resistance Promotes Fitness of Methicillin-Resistant Staphylococcus aureus during Urinary Tract Infection. mBio 12, e0203821. 10.1128/mBio.02038-21 Paudel S, Bagale K, Patel S, Kooyers NJ, Kulkarni R (2021) Human Urine Alters Methicillin-Resistant Staphylococcus aureus Virulence and Transcriptome. Appl Environ Microbiol 87:e0074421. 10.1128/aem.00744-21 Mustapha MM et al (2022) Genomic Diversity of Hospital-Acquired Infections Revealed through Prospective Whole-Genome Sequencing-Based Surveillance. mSystems 7, e0138421. 10.1128/msystems.01384-21 Cespedes C et al (2002) Differences between Staphylococcus aureus isolates from medical and nonmedical hospital personnel. J Clin Microbiol 40:2594–2597. 10.1128/jcm.40.7.2594-2597.2002 Alshomrani MK, Alharbi AA, Alshehri AA, Arshad M, Dolgum S (2023) Isolation of Staphylococcus aureus Urinary Tract Infections at a Community-Based Healthcare Center in Riyadh. Cureus 15:e35140. 10.7759/cureus.35140 Goyal M et al (2019) Genomic Evolution of Staphylococcus aureus During Artificial and Natural Colonization of the Human Nose. Front Microbiol 10:1525. 10.3389/fmicb.2019.01525 Young BC et al (2012) Evolutionary dynamics of Staphylococcus aureus during progression from carriage to disease. Proc Natl Acad Sci U S A 109:4550–4555. 10.1073/pnas.1113219109 Kaur K et al (2014) Evaluation of efficacy and tolerability of cefotaxime and sulbactam versus cefepime and tazobactam in patients of urinary tract infection-a prospective comparative study. J Clin Diagn Res 8:Hc05–08. 10.7860/jcdr/2014/9742.5090 Wayne P (2017) CLSI Performance Standards for Antimicrobial Susceptibility Testing. CLSI Document Clinical Laboratory Standards Institute (CLSI): Wayne, PA, USA van Duin D et al (2020) Molecular and clinical epidemiology of carbapenem-resistant Enterobacterales in the USA (CRACKLE-2): a prospective cohort study. Lancet Infect Dis 20:731–741. 10.1016/s1473-3099(19)30755-8 Manni M, Berkeley MR, Seppey M, Simão FA, Zdobnov EM (2021) BUSCO Update: Novel and Streamlined Workflows along with Broader and Deeper Phylogenetic Coverage for Scoring of Eukaryotic, Prokaryotic, and Viral Genomes. Mol Biol Evol 38:4647–4654. 10.1093/molbev/msab199 Parks DH, Imelfort M, Skennerton CT, Hugenholtz P, Tyson GW (2015) CheckM: assessing the quality of microbial genomes recovered from isolates, single cells, and metagenomes. Genome Res 25:1043–1055. 10.1101/gr.186072.114 Roosaare M et al (2017) StrainSeeker: fast identification of bacterial strains from raw sequencing reads using user-provided guide trees. PeerJ 5:e3353. 10.7717/peerj.3353 Duran Ramirez JM et al (2023) Staphylococcus aureus Breast Implant Infection Isolates Display Recalcitrance To Antibiotic Pocket Irrigants. Microbiol Spectr 11:e0288422. 10.1128/spectrum.02884-22 Jolley KA, Maiden MC, BIGSdb (2010) Scalable analysis of bacterial genome variation at the population level. BMC Bioinformatics 11:595. 10.1186/1471-2105-11-595 Seemann T (2014) Prokka: rapid prokaryotic genome annotation. Bioinformatics 30:2068–2069. 10.1093/bioinformatics/btu153 Page AJ et al (2015) Roary: rapid large-scale prokaryote pan genome analysis. Bioinformatics 31:3691–3693. 10.1093/bioinformatics/btv421 Contreras GA et al (2022) Contemporary Clinical and Molecular Epidemiology of Vancomycin-Resistant Enterococcal Bacteremia: A Prospective Multicenter Cohort Study (VENOUS I). Open Forum Infect Dis 9:ofab616. 10.1093/ofid/ofab616 Stamatakis A (2014) RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics 30:1312–1313. 10.1093/bioinformatics/btu033 Letunic I, Bork P (2019) Interactive Tree Of Life (iTOL) v4: recent updates and new developments. Nucleic Acids Res 47:W256–w259. 10.1093/nar/gkz239 Seemann T (2015) Snippy: rapid haploid variant calling and core SNP phylogeny. GitHub. Available at: github. com/tseemann/snippy Feldgarden M et al (2021) AMRFinderPlus and the Reference Gene Catalog facilitate examination of the genomic links among antimicrobial resistance, stress response, and virulence. Sci Rep 11:12728. 10.1038/s41598-021-91456-0 Sakurai A et al (2023) Evolving landscape of carbapenem-resistant Pseudomonas aeruginosa at a single centre in the USA. JAC Antimicrob Resist 5:dlad070. 10.1093/jacamr/dlad070 Seemann T (2016) ABRicate: mass screening of contigs for antibiotic resistance genes. San Francisco CA: GitHub Kaya H et al (2018) SCCmecFinder, a Web-Based Tool for Typing of Staphylococcal Cassette Chromosome mec in Staphylococcus aureus Using Whole-Genome Sequence Data. mSphere 3. 10.1128/mSphere.00612-17 Additional Declarations There is NO Competing Interest. Supplementary Files DuranRamirezSupplementalFigures.docx Supplemental Figures DuranRamirezSupplementaryFile.xlsx Supplementary file Cite Share Download PDF Status: Published Journal Publication published 13 Jan, 2026 Read the published version in Nature Communications → Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-6702271","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Article","associatedPublications":[],"authors":[{"id":462975956,"identity":"6a25e8be-05ce-4919-95c3-f29d83ddb562","order_by":0,"name":"Jennifer Walker","email":"data:image/png;base64,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","orcid":"https://orcid.org/0000-0002-0079-3261","institution":"The University of Texas Health Science Center","correspondingAuthor":true,"prefix":"","firstName":"Jennifer","middleName":"","lastName":"Walker","suffix":""},{"id":462975957,"identity":"a6db07cb-894a-4031-9b57-8edbdccab0ea","order_by":1,"name":"Jesus Duran Ramirez","email":"","orcid":"","institution":"The University of Texas Health Science Center","correspondingAuthor":false,"prefix":"","firstName":"Jesus","middleName":"Duran","lastName":"Ramirez","suffix":""},{"id":462975958,"identity":"21f350d8-874f-4b56-bb5b-404004a7bdc5","order_by":2,"name":"Chelsie Armbruster","email":"","orcid":"https://orcid.org/0000-0002-2165-2759","institution":"University at Buffalo, State University of New York","correspondingAuthor":false,"prefix":"","firstName":"Chelsie","middleName":"","lastName":"Armbruster","suffix":""},{"id":462975959,"identity":"886f37b1-b473-4623-b071-2cc6a20eab3e","order_by":3,"name":"Blake Hanson","email":"","orcid":"https://orcid.org/0000-0003-2949-7818","institution":"University of Texas Health Science Center at Houston","correspondingAuthor":false,"prefix":"","firstName":"Blake","middleName":"","lastName":"Hanson","suffix":""}],"badges":[],"createdAt":"2025-05-19 23:35:11","currentVersionCode":1,"declarations":"","doi":"10.21203/rs.3.rs-6702271/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-6702271/v1","draftVersion":[],"editorialEvents":[{"content":"https://doi.org/10.1038/s41467-025-68081-w","type":"published","date":"2026-01-13T05:00:00+00:00"}],"editorialNote":"","failedWorkflow":false,"files":[{"id":83612820,"identity":"1f026681-e5dd-476b-a87e-b2d17dd14a0f","added_by":"auto","created_at":"2025-05-29 12:47:16","extension":"jpeg","order_by":1,"title":"Figure 1","display":"","copyAsset":false,"role":"figure","size":628807,"visible":true,"origin":"","legend":"\u003cp\u003eSample collection period of \u003cem\u003eS. aureus\u003c/em\u003e mono- or poly-microbial urinary tract colonization, antimicrobial exposures, diagnosed \u003cem\u003eS. aureus \u003c/em\u003ecatheter-associated urinary tract infection (CAUTI), and catheter exchanges. \u0026nbsp;\u003cstrong\u003eA)\u003c/strong\u003e The urology outpatient (UP) isolates were collected from catheter and urine samples during regularly scheduled, monthly catheter exchanges. A total of 38 longitudinal \u003cem\u003eS. aureus \u003c/em\u003eisolates were collected and two individuals experienced symptomatic \u003cem\u003eS. aureus \u003c/em\u003eCAUTI. \u003cstrong\u003eB)\u003c/strong\u003e The nursing home resident (NHR) isolates were collected from weekly urine samples. A total of 133 \u003cem\u003eS. aureus \u003c/em\u003estrains were collected and no individual experienced a symptomatic \u003cem\u003eS. aureus \u003c/em\u003eCAUTI.\u003c/p\u003e","description":"","filename":"image1.jpeg","url":"https://assets-eu.researchsquare.com/files/rs-6702271/v1/a109bdf4efde7c0d67d6b583.jpeg"},{"id":83612600,"identity":"55147b79-342a-4501-ae8c-45c05c7a2dc5","added_by":"auto","created_at":"2025-05-29 12:39:16","extension":"jpeg","order_by":2,"title":"Figure 2","display":"","copyAsset":false,"role":"figure","size":551285,"visible":true,"origin":"","legend":"\u003cp\u003ePhylogenetic analysis of longitudinal \u003cem\u003eS. aureus\u003c/em\u003e isolates collected from individuals with long-term urinary catheters. The phylogenetic tree displays the participant ID (PT ID), the cohort ID and collection time point (CO ID), sample type (SID), and the presence or absence of \u003cem\u003emecA\u003c/em\u003e (\u003cem\u003emecA\u003c/em\u003e) moving from the inner to the outer rings, and the multi-locus sequence type (ST) within the branches of the tree. Isolates cluster first by ST type, and then within each ST by participant.\u003c/p\u003e","description":"","filename":"image2.jpeg","url":"https://assets-eu.researchsquare.com/files/rs-6702271/v1/afe821624f8d8233528eba4f.jpeg"},{"id":83612603,"identity":"99622271-ec9e-444e-a560-7c8ff1cc7d46","added_by":"auto","created_at":"2025-05-29 12:39:16","extension":"jpeg","order_by":3,"title":"Figure 3","display":"","copyAsset":false,"role":"figure","size":166447,"visible":true,"origin":"","legend":"\u003cp\u003eChanges in accessory genes over time. Changes in the accessory genomes are shown as changes from the index isolates: a positive number indicates a gain of genes, a negative number denotes a loss of genes, a value of 0 represents no change. Black bars represent comparisons of longitudinal isolates with the same ST while hatched bars represent strains with a new ST (strain switching). Most within-participant isolates maintained a similar accessory genome over time.\u003c/p\u003e","description":"","filename":"image3.jpeg","url":"https://assets-eu.researchsquare.com/files/rs-6702271/v1/bf08a073088cdc0e03399e5a.jpeg"},{"id":83612604,"identity":"513b38ba-7f6d-4170-8b3b-a7730beb7c2b","added_by":"auto","created_at":"2025-05-29 12:39:16","extension":"jpeg","order_by":4,"title":"Figure 4","display":"","copyAsset":false,"role":"figure","size":126699,"visible":true,"origin":"","legend":"\u003cp\u003eSingle nucleotide polymorphism (SNP) analysis. Comparisons include all isolates between participants (n=3344, median = 2344), all isolates of the same sequence type (ST) between participants (n=1302, median =1120), all isolates within a participant (n=148, median = 8), and isolates of the same ST within a participant (n=131, median = 7). Box and whisker plots display the median as a black bar within the interquartile range (IQR) box, with the whiskers representing the range. Isolates from the same participant and same ST have the fewest median SNPs. The Mann-Whitney U test was used to determine statistical significance, where **** indicates P \u0026lt; 0.0001, and ns indicates no significances and P\u0026gt;0.05.\u003c/p\u003e","description":"","filename":"image4.jpeg","url":"https://assets-eu.researchsquare.com/files/rs-6702271/v1/d82d9f570836a5377c81c6d6.jpeg"},{"id":83612606,"identity":"06c91ecb-6501-4360-ab36-f400b1dec237","added_by":"auto","created_at":"2025-05-29 12:39:16","extension":"jpeg","order_by":5,"title":"Figure 5","display":"","copyAsset":false,"role":"figure","size":682577,"visible":true,"origin":"","legend":"\u003cp\u003eAntimicrobial resistance (AMR) gene carriage profile of \u003cem\u003eS. aureus \u003c/em\u003estrains collected from individuals with long-term urinary catheters\u003cem\u003e. S. aureus\u003c/em\u003eisolates are organized horizontally in chronological order with each strain labeled. The AMR genes are shown on top and are organized based on their associated antibiotic class. Antibiotic class is labeled below the head map and are organized in alphabetical order in which Aminoglycosides (AMG), Beta-Lactams (BET), Bleomycin (BLE), Fosfomycin (FOS), Lincosamide (LIN), Macrolide (MAC), Mupirocin (MUP), Quinolone (QUI), Rifamycin (RIF), Streprothricin (STR), and Tetracycline as (TE) based on AMRFinderPlus classification. Bars to the right of the graph indicate phenotypic methicillin resistance (black bars) and susceptibility (white bars) based on CSLI guidelines against oxacillin or cefoxitin. The following abbreviations were used glptT1 (glpT_A400V), glptT2 (glpT_V243I), murA_1 (murA_D278E), murA_2 (murA_E294D), murA_3 (murA_G257D), murA_4 (murA_T396N), ileS1 (ileS_V588F), gyRA1 (gyrA_S84L), gyRA2 (gyrA_S85P), parC1 (parC_E84G), parC2 (parC_E84K), parC3 (parC_S80F), parC4 (parC_S80Y), parE1 (parE_D432N), and rpoB1 (rpoB_H489N).\u003c/p\u003e","description":"","filename":"image5.jpeg","url":"https://assets-eu.researchsquare.com/files/rs-6702271/v1/71fd09288e9ad6b8d444e110.jpeg"},{"id":83612607,"identity":"285ea618-b497-47e9-81b3-f2f6ca46e8bd","added_by":"auto","created_at":"2025-05-29 12:39:16","extension":"jpeg","order_by":6,"title":"Figure 6","display":"","copyAsset":false,"role":"figure","size":114370,"visible":true,"origin":"","legend":"\u003cp\u003eCorrelation analysis between antimicrobial resistance (AMR) determinants and virulence genes. The total number of “resistance” determinants identified by AMRFinderplus were plotted against the total number of “virulence” genes identified by VFDB. A nonparametric Spearman’s correlation analysis was performed, and a significant correlation was found with p = 0.0347 and a r value of -0.442.\u003c/p\u003e","description":"","filename":"image6.jpeg","url":"https://assets-eu.researchsquare.com/files/rs-6702271/v1/114110b31fde0813a4dc8f77.jpeg"},{"id":101934430,"identity":"14fe2b9b-8c89-4d5a-8193-c88b52f663ef","added_by":"auto","created_at":"2026-02-05 08:14:42","extension":"pdf","order_by":0,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":3456669,"visible":true,"origin":"","legend":"","description":"","filename":"manuscript.pdf","url":"https://assets-eu.researchsquare.com/files/rs-6702271/v1/538233f3-6ff8-4a12-abc3-2eea8cee1be7.pdf"},{"id":83612819,"identity":"27c893e7-582e-4905-b986-4cf5a2cc0df0","added_by":"auto","created_at":"2025-05-29 12:47:16","extension":"docx","order_by":1,"title":"","display":"","copyAsset":false,"role":"supplement","size":2209959,"visible":true,"origin":"","legend":"Supplemental Figures","description":"","filename":"DuranRamirezSupplementalFigures.docx","url":"https://assets-eu.researchsquare.com/files/rs-6702271/v1/9d644b4d2bc1d98ec3592d7f.docx"},{"id":83612609,"identity":"e80b5258-a3fd-4b8a-b5cc-f951327d557a","added_by":"auto","created_at":"2025-05-29 12:39:17","extension":"xlsx","order_by":2,"title":"","display":"","copyAsset":false,"role":"supplement","size":3647508,"visible":true,"origin":"","legend":"Supplementary file","description":"","filename":"DuranRamirezSupplementaryFile.xlsx","url":"https://assets-eu.researchsquare.com/files/rs-6702271/v1/e893696bedc8b4b4889c6d08.xlsx"}],"financialInterests":"There is \u003cb\u003eNO\u003c/b\u003e Competing Interest.","formattedTitle":"Genomics Reveal Staphylococcus aureus Persists during Long-term Urinary Catheterization Despite Antimicrobial Therapy and Catheter Exchanges","fulltext":[{"header":"Introduction","content":"\u003cp\u003eUrinary catheters are the most frequently placed medical devices in the United States, with \u0026gt;\u0026thinsp;30\u0026nbsp;million used annually\u003csup\u003e\u003cspan citationid=\"CR1\" class=\"CitationRef\"\u003e1\u003c/span\u003e,\u003cspan citationid=\"CR2\" class=\"CitationRef\"\u003e2\u003c/span\u003e\u003c/sup\u003e. Their widespread use is due to their efficacy in the clinical management of hospitalized and critically ill patients, of which 25% and 60% receive a urinary catheter, respectively\u003csup\u003e\u003cspan citationid=\"CR3\" class=\"CitationRef\"\u003e3\u003c/span\u003e\u003c/sup\u003e. Urinary catheters are also extremely effective at improving the quality of life for millions of individuals, including those with neurogenic bladder and the elderly\u003csup\u003e\u003cspan citationid=\"CR4\" class=\"CitationRef\"\u003e4\u003c/span\u003e,\u003cspan citationid=\"CR5\" class=\"CitationRef\"\u003e5\u003c/span\u003e\u003c/sup\u003e. However, urinary catheters increase the risk of developing symptomatic catheter-associated urinary tract infections (CAUTIs) \u0026ndash; with individuals requiring long-term (\u0026gt;\u0026thinsp;28 days) urinary catheters (LTUCs) at highest risk\u003csup\u003e\u003cspan citationid=\"CR6\" class=\"CitationRef\"\u003e6\u003c/span\u003e,\u003cspan citationid=\"CR7\" class=\"CitationRef\"\u003e7\u003c/span\u003e\u003c/sup\u003e. Additionally, urinary catheters increase the incidence of asymptomatic bacteriuria (ASB) \u0026ndash; the presence of bacteria in the urine without symptoms\u003csup\u003e\u003cspan citationid=\"CR3\" class=\"CitationRef\"\u003e3\u003c/span\u003e\u003c/sup\u003e. Persistent ASB, particularly among those with LTUCs, also increases the risk of developing symptomatic CAUTI\u003csup\u003e\u003cspan citationid=\"CR8\" class=\"CitationRef\"\u003e8\u003c/span\u003e,\u003cspan citationid=\"CR9\" class=\"CitationRef\"\u003e9\u003c/span\u003e\u003c/sup\u003e. Challengingly, studies demonstrate that prophylactic antibiotic treatment of individuals with urinary catheters does not eliminate ASB nor does it prevent catheter colonization, even during short-term (\u0026lt;\u0026thinsp;28 days) catheterization\u003csup\u003e\u003cspan citationid=\"CR3\" class=\"CitationRef\"\u003e3\u003c/span\u003e,\u003cspan additionalcitationids=\"CR11\" citationid=\"CR10\" class=\"CitationRef\"\u003e10\u003c/span\u003e\u0026ndash;\u003cspan citationid=\"CR12\" class=\"CitationRef\"\u003e12\u003c/span\u003e\u003c/sup\u003e. Prophylactic antibiotic treatment is, however, associated with the development of antimicrobial resistance (AMR), as well as other serious illnesses, including \u003cem\u003eClostridioides difficile\u003c/em\u003e colitis\u003csup\u003e\u003cspan citationid=\"CR13\" class=\"CitationRef\"\u003e13\u003c/span\u003e,\u003cspan citationid=\"CR14\" class=\"CitationRef\"\u003e14\u003c/span\u003e\u003c/sup\u003e. Additional studies indicate empiric antibiotic treatment of CAUTI also does not improve patient outcomes\u003csup\u003e\u003cspan citationid=\"CR15\" class=\"CitationRef\"\u003e15\u003c/span\u003e,\u003cspan citationid=\"CR16\" class=\"CitationRef\"\u003e16\u003c/span\u003e\u003c/sup\u003e. Thus, management of ASB and prevention and treatment of CAUTI remain difficult.\u003c/p\u003e \u003cp\u003eDue to the high infection rates of urinary catheters (10\u0026ndash;30%) combined with a lack of prevention strategies, CAUTIs have become the most common hospital-associated infections in the U.S., accounting for \u0026gt;\u0026thinsp;1\u0026nbsp;million cases and resulting in as much as \u003cspan\u003e$\u003c/span\u003e1.83\u0026nbsp;billion/year in associated preventable costs\u003csup\u003e \u003cspan additionalcitationids=\"CR18\" citationid=\"CR17\" class=\"CitationRef\"\u003e17\u003c/span\u003e\u0026ndash;\u003cspan citationid=\"CR19\" class=\"CitationRef\"\u003e19\u003c/span\u003e \u003c/sup\u003e. CAUTIs can also progress to severe disease, including bacteremia and sepsis, which increases healthcare costs, length of hospital stay, morbidity, and mortality\u003csup\u003e \u003cspan additionalcitationids=\"CR21\" citationid=\"CR20\" class=\"CitationRef\"\u003e20\u003c/span\u003e\u0026ndash;\u003cspan citationid=\"CR22\" class=\"CitationRef\"\u003e22\u003c/span\u003e \u003c/sup\u003e. Both CAUTI and ASB are caused by a broad range of uropathogens, including Gram-negatives such as \u003cem\u003eEscherichia coli, Klebsiella pneumoniae\u003c/em\u003e, and \u003cem\u003ePseudomonas aeruginosa\u003c/em\u003e, as well as Gram-positives like \u003cem\u003eEnterococcus faecalis\u003c/em\u003e and \u003cem\u003eStaphylococcus aureus\u003c/em\u003e \u003csup\u003e \u003cspan citationid=\"CR23\" class=\"CitationRef\"\u003e23\u003c/span\u003e,\u003cspan citationid=\"CR24\" class=\"CitationRef\"\u003e24\u003c/span\u003e \u003c/sup\u003e. Increasing AMR among these uropathogens not only limits treatment options, but it further complicates empiric treatment\u003csup\u003e \u003cspan citationid=\"CR25\" class=\"CitationRef\"\u003e25\u003c/span\u003e,\u003cspan citationid=\"CR26\" class=\"CitationRef\"\u003e26\u003c/span\u003e \u003c/sup\u003e. Additionally, CAUTI and ASB in individuals with LTUCs are typically polymicrobial, making it difficult for physicians to differentiate uropathogens that cause symptoms from those that are asymptomatically colonizing\u003csup\u003e \u003cspan citationid=\"CR27\" class=\"CitationRef\"\u003e27\u003c/span\u003e,\u003cspan citationid=\"CR28\" class=\"CitationRef\"\u003e28\u003c/span\u003e \u003c/sup\u003e. Despite the significant challenges associated with identification and treatment of these diverse uropathogens, the majority remain understudied. Understanding how these uropathogens persist as ASB despite prophylactic antibiotic administration is critical for developing and implementing treatment strategies that reduce the disease burden associated with these common and costly infections.\u003c/p\u003e \u003cp\u003eIn this study, we focus on the understudied uropathogen \u003cem\u003eS. aureus\u003c/em\u003e. Previous studies demonstrate that \u003cem\u003ei) S. aureus\u003c/em\u003e within the urinary tract is primarily associated with urinary catheterization, \u003cem\u003eii)\u003c/em\u003e for those with persistent \u003cem\u003eS. aureus\u003c/em\u003e ASB, one third will go on to develop a symptomatic UTI, and \u003cem\u003eiii)\u003c/em\u003e UTI symptoms caused by \u003cem\u003eS. aureus\u003c/em\u003e are typically more severe, with higher rates of dissemination to secondary bacteremia, compared to other uropathogens\u003csup\u003e\u003cspan citationid=\"CR9\" class=\"CitationRef\"\u003e9\u003c/span\u003e,\u003cspan additionalcitationids=\"CR30 CR31 CR32\" citationid=\"CR29\" class=\"CitationRef\"\u003e29\u003c/span\u003e\u0026ndash;\u003cspan citationid=\"CR33\" class=\"CitationRef\"\u003e33\u003c/span\u003e\u003c/sup\u003e. Additionally, recent studies indicate \u003cem\u003eS. aureus\u003c/em\u003e is a common asymptomatic colonizer of LTUCs; yet there is a paucity of studies investigating the lifecycle of this pathogen in the urinary tract, particularly within LTUCs\u003csup\u003e\u003cspan citationid=\"CR3\" class=\"CitationRef\"\u003e3\u003c/span\u003e,\u003cspan citationid=\"CR11\" class=\"CitationRef\"\u003e11\u003c/span\u003e\u003c/sup\u003e. We used whole-genome sequencing to analyze a collection of \u003cem\u003eS. aureus\u003c/em\u003e strains previously isolated from 20 individuals with LTUCs\u003csup\u003e\u003cspan citationid=\"CR3\" class=\"CitationRef\"\u003e3\u003c/span\u003e,\u003cspan citationid=\"CR11\" class=\"CitationRef\"\u003e11\u003c/span\u003e\u003c/sup\u003e. A total of 171 strains, with an average of 8 longitudinal isolates collected per person over an average collection duration of 9 months, were included in these analyses. We discovered that most individuals were colonized with methicillin resistant \u003cem\u003eS. aureus\u003c/em\u003e (MRSA) belonging to multi-locus sequence type (ST) 5. Notably, most participants received multiple antibiotics over the course of the collection period, with nitrofurantoin and ciprofloxacin the two most commonly prescribed\u003csup\u003e\u003cspan citationid=\"CR3\" class=\"CitationRef\"\u003e3\u003c/span\u003e,\u003cspan citationid=\"CR11\" class=\"CitationRef\"\u003e11\u003c/span\u003e\u003c/sup\u003e. Antimicrobial susceptibility testing revealed all \u003cem\u003eS. aureus\u003c/em\u003e isolates tested were susceptible to nitrofurantoin, but \u0026gt;\u0026thinsp;60% were resistant to ciprofloxacin. Despite anti-staphylococcal antibiotic exposure and frequent catheter exchanges, longitudinal isolates from a given participant \u0026ndash; termed within-participant \u0026ndash; were highly genetically related, with a median of four changes (gene gain/loss) to the accessory genomes and a median pairwise SNP distance of seven single nucleotide polymorphisms (SNPs). Virulence factor and AMR determinant carriage was also stable among within-participant isolates. Furthermore, two participants developed symptomatic \u003cem\u003eS. aureus\u003c/em\u003e CAUTI during the collection period, of which one of the \u003cem\u003eS. aureus\u003c/em\u003e isolates was detected as ASB for months prior to and after the symptomatic events. These results suggest that the two strategies used most frequently to try to reduce or eliminate ASB (antimicrobial administration\u003csup\u003e\u003cspan citationid=\"CR34\" class=\"CitationRef\"\u003e34\u003c/span\u003e\u003c/sup\u003e and catheter exchange\u003csup\u003e\u003cspan citationid=\"CR7\" class=\"CitationRef\"\u003e7\u003c/span\u003e\u003c/sup\u003e) are ineffective at preventing \u003cem\u003eS. aureus\u003c/em\u003e from persisting in individuals with LTUCs. Collectively, this study demonstrates \u003cem\u003eS. aureus\u003c/em\u003e persistently colonizes the catheterized urinary tract, serving as a reservoir for AMR makers and subsequent symptomatic infection.\u003c/p\u003e"},{"header":"Results","content":"\u003cp\u003e \u003cb\u003eEpidemiology of\u003c/b\u003e \u003cb\u003eS. aureus\u003c/b\u003e \u003cb\u003eisolates from individuals with LTUCs\u003c/b\u003e\u003c/p\u003e \u003cp\u003e \u003cem\u003eS. aureus\u003c/em\u003e strains were longitudinally isolated from individuals with LTUCs from two separate, previously published cohorts\u003csup\u003e\u003cspan citationid=\"CR3\" class=\"CitationRef\"\u003e3\u003c/span\u003e,\u003cspan citationid=\"CR11\" class=\"CitationRef\"\u003e11\u003c/span\u003e,\u003cspan citationid=\"CR12\" class=\"CitationRef\"\u003e12\u003c/span\u003e\u003c/sup\u003e. The first cohort consists of urology outpatients (UPs) from a single outpatient clinic\u003csup\u003e\u003cspan citationid=\"CR11\" class=\"CitationRef\"\u003e11\u003c/span\u003e,\u003cspan citationid=\"CR12\" class=\"CitationRef\"\u003e12\u003c/span\u003e\u003c/sup\u003e. For this cohort, microbes, including \u003cem\u003eS. aureus\u003c/em\u003e, were cultured monthly from urine and LTUCs for approximately 12 sampling time points. The second cohort consists of nursing home residents (NHRs) from two long-term care facilities\u003csup\u003e\u003cspan citationid=\"CR3\" class=\"CitationRef\"\u003e3\u003c/span\u003e\u003c/sup\u003e. For this cohort, microbes, including \u003cem\u003eS. aureus\u003c/em\u003e, were cultured weekly from urine samples for up to 27 sampling time points. While the sample collection type and sites of each cohort differed, the average participant age was similar, 63.4 (\u0026plusmn; 15.5 years) for the UP and 65 (\u0026plusmn;15 years) for the NHR cohorts\u003csup\u003e\u003cspan citationid=\"CR3\" class=\"CitationRef\"\u003e3\u003c/span\u003e,\u003cspan citationid=\"CR11\" class=\"CitationRef\"\u003e11\u003c/span\u003e\u003c/sup\u003e. Additionally, each cohort consisted of 10 participants that were colonized with \u003cem\u003eS. aureus\u003c/em\u003e during at least one sampling time point (Table\u0026nbsp;\u003cspan refid=\"Tab1\" class=\"InternalRef\"\u003e1\u003c/span\u003e). For the 10 UPs, they received an average of 2.9 antibiotic treatments per participant, 50% were female, and \u003cem\u003eS. aureus\u003c/em\u003e strains were collected on average every 27.4 days at the time of the scheduled catheter exchange. For the 10 NHRs, they received an average of 1.5 antibiotic treatments per participant, 20% were female, and \u003cem\u003eS. aureus\u003c/em\u003e strains were collected on average every seven days \u0026ndash; with catheter exchanges occurring every 38.12 days on average. There were no significant differences observed for the gender, antibiotic exposures, or catheter duration between the UPs and NHRs colonized with \u003cem\u003eS. aureus\u003c/em\u003e. Overall, the demographics and epidemiology of individuals with \u003cem\u003eS. aureus\u003c/em\u003e colonization within both cohorts were similar.\u003c/p\u003e \u003cp\u003e \u003cdiv class=\"gridtable\"\u003e\u003ctable float=\"Yes\" id=\"Tab1\" border=\"1\"\u003e \u003ccaption language=\"En\"\u003e \u003cdiv class=\"CaptionNumber\"\u003eTable 1\u003c/div\u003e \u003cdiv class=\"CaptionContent\"\u003e \u003cp\u003eParticipant demographics and epidemiology of clinical isolates from participants with \u003cem\u003eS. aureus\u003c/em\u003e colonization of long-term urinary catheters. Statistical analyses were performed using the Fisher\u0026rsquo;s exact test denoted with * or the Mann-Whitney test denoted with \u003csup\u003e#\u003c/sup\u003e.\u003c/p\u003e \u003c/div\u003e \u003c/caption\u003e \u003ccolgroup cols=\"4\"\u003e \u003cdiv align=\"left\" class=\"colspec\" colname=\"c1\" colnum=\"1\"\u003e\u003c/div\u003e \u003cdiv align=\"left\" class=\"colspec\" colname=\"c2\" colnum=\"2\"\u003e\u003c/div\u003e \u003cdiv align=\"left\" class=\"colspec\" colname=\"c3\" colnum=\"3\"\u003e\u003c/div\u003e \u003cdiv align=\"left\" class=\"colspec\" colname=\"c4\" colnum=\"4\"\u003e\u003c/div\u003e \u003cthead\u003e \u003ctr\u003e \u003cth align=\"left\" colname=\"c1\"\u003e\u0026nbsp;\u003c/th\u003e \u003cth align=\"left\" colname=\"c2\"\u003e \u003cp\u003eUrology Outpatients\u003c/p\u003e \u003cp\u003e(UP)\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c3\"\u003e \u003cp\u003eNursing Home Resident (NHR)\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c4\"\u003e \u003cp\u003ep-value\u003c/p\u003e \u003c/th\u003e \u003c/tr\u003e \u003c/thead\u003e \u003ctbody\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eParticipants with \u003cem\u003eS. aureus\u003c/em\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003e10\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003e10\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003eNA\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eFemale Participants\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003e50%\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003e20%\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003e0.3498*\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003ePercent of Participants with MRSA Colonization\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003e40%\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003e60%\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003e0.6563*\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eAverage Antibiotic Treatments per participant\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003e2.9\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003e1.5\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003e0.2867#\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eAverage Catheterization Duration\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003e27.40 days\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003e38.12 days\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003e0.6698#\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eNumber of \u003cem\u003eS. aureus\u003c/em\u003e Isolates\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003e38\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003e133\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003eNA\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eAverage Number of \u003cem\u003eS. aureus\u003c/em\u003e isolates per participant\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003e3.8\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003e13.3\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003eNA\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003c/tbody\u003e \u003c/colgroup\u003e \u003c/table\u003e\u003c/div\u003e \u003c/p\u003e \u003cp\u003e \u003cb\u003eS. aureus\u003c/b\u003e \u003cb\u003eLTUC isolate collection\u003c/b\u003e\u003c/p\u003e \u003cp\u003eA total of 171 \u003cem\u003eS. aureus\u003c/em\u003e isolates were collected from 273 sampling time points across these 20 participants (\u003cb\u003eTable \u003cspan refid=\"MOESM1\" class=\"InternalRef\"\u003eS1\u003c/span\u003e\u003c/b\u003e). Most participants had a single isolate per species collected at each time point. However, several individuals had multiple \u003cem\u003eS. aureus\u003c/em\u003e strains collected within a single time point \u0026ndash; defined as co-isolated. These co-isolated strains were collected based on phenotypic differences, including size, color, rugosity, and antibiotic resistance, and were more frequent in the UP cohort due to culturing of both urine and catheter samples\u003csup\u003e\u003cspan citationid=\"CR3\" class=\"CitationRef\"\u003e3\u003c/span\u003e,\u003cspan citationid=\"CR11\" class=\"CitationRef\"\u003e11\u003c/span\u003e\u003c/sup\u003e. Whole-genome sequencing confirmed all 171 strains were \u003cem\u003eS. aureus\u003c/em\u003e (\u003cb\u003eTable \u003cspan refid=\"MOESM1\" class=\"InternalRef\"\u003eS1\u003c/span\u003e-S3\u003c/b\u003e). Within the UPs, 38 \u003cem\u003eS. aureus\u003c/em\u003e isolates were sequenced, averaging 3.8 strains per participant (Fig.\u0026nbsp;\u003cspan refid=\"Fig1\" class=\"InternalRef\"\u003e1\u003c/span\u003e, Tables\u0026nbsp;\u003cspan refid=\"Tab1\" class=\"InternalRef\"\u003e1\u003c/span\u003e and S1-S3). Within the NHRs, 133 strains were sequenced, averaging 13.3 isolates per participant (Fig.\u0026nbsp;\u003cspan refid=\"Fig1\" class=\"InternalRef\"\u003e1\u003c/span\u003e, Tables\u0026nbsp;\u003cspan refid=\"Tab1\" class=\"InternalRef\"\u003e1\u003c/span\u003e and S1-S3). Notably, two participants within the UP cohort experienced symptomatic \u003cem\u003eS. aureus\u003c/em\u003e CAUTI\u003csup\u003e\u003cspan citationid=\"CR11\" class=\"CitationRef\"\u003e11\u003c/span\u003e\u003c/sup\u003e. None of the participants in the NHR cohort were diagnosed with CAUTI attributed to \u003cem\u003eS. aureus\u003c/em\u003e\u003csup\u003e\u003cspan citationid=\"CR3\" class=\"CitationRef\"\u003e3\u003c/span\u003e\u003c/sup\u003e. While the majority of samples collected from these 20 participants were polymicrobial, \u003cem\u003eS. aureus\u003c/em\u003e was observed to be monomicrobial at four separate time points (Fig.\u0026nbsp;\u003cspan refid=\"Fig1\" class=\"InternalRef\"\u003e1\u003c/span\u003e). Despite multiple antibiotic exposures and catheter exchanges over the course of sample collection, only two of the 273 samples collected had no detectable uropathogens across both cohorts. These results indicate that \u003cem\u003eS. aureus\u003c/em\u003e is a common, asymptomatic colonizer that can be mono-microbial within individuals with LTUCs.\u003c/p\u003e\u003cp\u003e \u003cb\u003eGenomic analyses of\u003c/b\u003e \u003cb\u003eS. aureus\u003c/b\u003e \u003cb\u003eLTUC co-isolated strains\u003c/b\u003e\u003c/p\u003e \u003cp\u003eTo determine the population heterogeneity of co-isolated strains and investigate whether strains isolated from the urine and LTUC were genetically related, we analyzed 29 co-isolated genomes from four patients across 10 time points (Fig.\u0026nbsp;\u003cspan refid=\"Fig1\" class=\"InternalRef\"\u003e1\u003c/span\u003e \u003cb\u003eand Table \u003cspan refid=\"MOESM1\" class=\"InternalRef\"\u003eS1\u003c/span\u003e\u003c/b\u003e): NHR 201 at collection 9 (n\u0026thinsp;=\u0026thinsp;2; both urine), 12 (n\u0026thinsp;=\u0026thinsp;2; both urine), and 14 (n\u0026thinsp;=\u0026thinsp;2; both urine); UP 86 at collection 7 (n\u0026thinsp;=\u0026thinsp;2; 1 catheter, 1 urine) and 10 (n\u0026thinsp;=\u0026thinsp;2; both catheter); UP 111 at collection 0 (n\u0026thinsp;=\u0026thinsp;2; 1 catheter, 1 urine); and UP 119 at collection 0 (n\u0026thinsp;=\u0026thinsp;2; 1 catheter, 1 urine), 7 (n\u0026thinsp;=\u0026thinsp;9; 6 catheter, 3 urine), 8 (n\u0026thinsp;=\u0026thinsp;4; 1 catheter, 3 urine), and 10 (n\u0026thinsp;=\u0026thinsp;2; 1 catheter, 1 urine). Strains isolated from the same collection time point were the same ST (\u003cb\u003eTable S2\u003c/b\u003e). Additionally, analyses of the accessory genome and SNPs (\u003cb\u003eTable S4-5\u003c/b\u003e) revealed that co-isolates displayed a median of 2.5 (IQR 1-12.5) accessory genome changes and a median pairwise SNP distance of five (IQR 3\u0026ndash;7), regardless of culture site or colony phenotype (\u003cb\u003eFigure \u003cspan refid=\"MOESM1\" class=\"InternalRef\"\u003eS1\u003c/span\u003eA\u003c/b\u003e and \u003cb\u003eS1B\u003c/b\u003e). Notably, both MRSA and methicillin-susceptible \u003cem\u003eS. aureus\u003c/em\u003e (MSSA) were co-isolated from NHR 201. In-depth analyses of genomic differences between the MRSA and MSSA co-isolates (n\u0026thinsp;=\u0026thinsp;2; two collection times) revealed that despite both lineages sharing the same ST (ST5), there was a median of 72 (IQR 69\u0026ndash;75) accessory genomes changes and a median pairwise SNP distance of 50 (\u003cb\u003eFigure \u003cspan refid=\"MOESM1\" class=\"InternalRef\"\u003eS1\u003c/span\u003eC\u003c/b\u003e and \u003cb\u003eS1D\u003c/b\u003e). These results suggest these co-isolates are from different lineages, despite isolation at the same collection time and sharing the same ST. Together, these findings indicate that most participants had a single, homogenous population of \u003cem\u003eS. aureus\u003c/em\u003e with few genomic differences that predominated at each collection time, and that the strain colonizing the catheter is highly genetically related to the strain colonizing the urine.\u003c/p\u003e \u003cp\u003e \u003cb\u003ePhylogenetic analyses of longitudinal\u003c/b\u003e \u003cb\u003eS. aureus\u003c/b\u003e \u003cb\u003eLTUC isolates\u003c/b\u003e\u003c/p\u003e \u003cp\u003eTo determine whether longitudinally collected \u003cem\u003eS. aureus\u003c/em\u003e LTUC isolates among both cohorts undergo frequent strain replacement \u0026ndash; due to antibiotic exposure and catheter exchanges \u0026ndash; or if they are the same strain persisting over time, a total of 153 longitudinal strains were selected for further analyses, representing a single strain from each participant at each collection time. Approximately 40% of UP and 60% of NHR strains carried \u003cem\u003emecA\u003c/em\u003e, classifying them as MRSA (Table\u0026nbsp;\u003cspan refid=\"Tab1\" class=\"InternalRef\"\u003e1\u003c/span\u003e \u003cb\u003eand\u003c/b\u003e Fig.\u0026nbsp;\u003cspan refid=\"Fig2\" class=\"InternalRef\"\u003e2\u003c/span\u003e). The most common lineage was ST5, which colonized 55% (11/20) of participants (Figs.\u0026nbsp;\u003cspan refid=\"Fig2\" class=\"InternalRef\"\u003e2\u003c/span\u003e, S2, and S3). The second most common lineage was ST45, which colonized 15% (3/20) of participants, followed by ST8 found in 10% (2/20) of participants. Impactfully, most participants (90%; 18/20) were colonized with the same ST over time (Figs.\u0026nbsp;\u003cspan refid=\"Fig1\" class=\"InternalRef\"\u003e1\u003c/span\u003e and \u003cspan refid=\"Fig2\" class=\"InternalRef\"\u003e2\u003c/span\u003e). There were only two instances (a single participant from each group) where the initial ST detected changed to a different ST at a later collection time: NHR 101 and UP 119. Phylogenetic analyses, using the well-characterized \u003cem\u003eS. aureus\u003c/em\u003e strain MRSA-1369 to root the tree, demonstrated that within-participant longitudinal isolates of the same ST clustered together (Figs.\u0026nbsp;\u003cspan refid=\"Fig2\" class=\"InternalRef\"\u003e2\u003c/span\u003e, S2, and S3). The longitudinal isolates with different STs \u0026ndash; from the participants that experienced the ST changes \u0026ndash; clustered separately. Additionally, participant isolates were observed to form sub-clusters based upon \u003cem\u003emecA\u003c/em\u003e carriage (Figs.\u0026nbsp;\u003cspan refid=\"Fig2\" class=\"InternalRef\"\u003e2\u003c/span\u003e and S3). Specifically, the longitudinal MSSA and MRSA isolates colonizing NHR 201 at alternating time points across collection times each formed distinct clusters, separate from one another. Together, these clustering patterns suggest that within-participant, longitudinal \u003cem\u003eS. aureus\u003c/em\u003e isolates are more closely related to each other than they are to isolates from other participants, even from the same ST. Importantly, this pattern was observed across geographically distinct cohorts, suggesting these results are broadly applicable to individuals with LTUCs colonized with \u003cem\u003eS. aureus\u003c/em\u003e.\u003c/p\u003e\u003cp\u003e \u003cb\u003eAccessory genome analyses of longitudinal\u003c/b\u003e \u003cb\u003eS. aureus\u003c/b\u003e \u003cb\u003eLTUC isolates\u003c/b\u003e\u003c/p\u003e \u003cp\u003eTo determine whether there were changes in the gene repertoire of longitudinal \u003cem\u003eS. aureus\u003c/em\u003e isolates over time, a pan-genome analysis was performed (\u003cb\u003eTable S4 and Figure S4\u003c/b\u003e). Changes to the accessory genome were determined by comparing the initial isolate to each subsequent longitudinal isolate. For within-participant longitudinal isolates with STs that differed from the index isolate (UP 119 and NHR 101), a median of 126 (IQR 118\u0026ndash;129) accessory genome changes were observed (Fig.\u0026nbsp;\u003cspan refid=\"Fig3\" class=\"InternalRef\"\u003e3\u003c/span\u003e). In contrast, comparisons between the first strain isolated of the same ST as the subsequent longitudinal isolates from UP 119 and NHR 101 revealed a median of 3 (IQR 1\u0026ndash;13) accessory genome changes. Thus, the first occurrence of a new ST was determined to be an index isolate for all subsequent longitudinal isolates that were the same ST. Importantly, comparisons of all longitudinal isolates to their respective index isolate demonstrated that a median of four (IQR 1\u0026ndash;19) accessory genome changes were observed (Fig.\u0026nbsp;\u003cspan refid=\"Fig3\" class=\"InternalRef\"\u003e3\u003c/span\u003e). Additionally, the NHR 201 index (MRSA) isolate compared to the five longitudinal MSSA strains indicate a median of 69 (IQR 67.50\u0026ndash;71.50) genes are absent from the MSSA genomes (Fig.\u0026nbsp;\u003cspan refid=\"Fig3\" class=\"InternalRef\"\u003e3\u003c/span\u003e and S5A). Most absent genes were associated with the SCC\u003cem\u003emecA\u003c/em\u003e Type II (2A) cassette, which is present in the MRSA isolates. Notably, comparing the first MSSA strain isolated (NHR 201-6) to the longitudinal MSSA strains, a median of 3 (IQR 1-6.5) accessory genome changes were observed (\u003cb\u003eFigure S5B\u003c/b\u003e). Together, these findings indicate that there are few changes to the accessory genomes of longitudinal isolates with the same ST and \u003cem\u003emecA\u003c/em\u003e carriage over time.\u003c/p\u003e \u003cp\u003e \u003cb\u003eSNP analyses of longitudinal\u003c/b\u003e \u003cb\u003eS. aureus\u003c/b\u003e \u003cb\u003eLTUC isolates\u003c/b\u003e\u003c/p\u003e \u003cp\u003eTo determine the genomic diversity of longitudinal \u003cem\u003eS. aureus\u003c/em\u003e isolates over time, SNP analyses were performed (Fig.\u0026nbsp;\u003cspan refid=\"Fig4\" class=\"InternalRef\"\u003e4\u003c/span\u003e \u003cb\u003eand Table S5\u003c/b\u003e). A median pairwise SNP distance of 2,344 (IQR 1178\u0026ndash;38777) was observed for all the index (first isolate of a unique ST) isolates between participants. For all isolates between participants with the same ST, the median pairwise SNP difference dropped to 1,120 (IQR 614\u0026ndash;1767), demonstrating that isolates from the same ST are more closely related to each other than to other STs (p-values\u0026thinsp;\u0026lt;\u0026thinsp;0.0001), as expected\u003csup\u003e\u003cspan citationid=\"CR35\" class=\"CitationRef\"\u003e35\u003c/span\u003e\u003c/sup\u003e. Notably, within-participant longitudinal isolates, including those with ST changes, displayed a median pairwise SNP difference of eight (IQR 3\u0026ndash;25). Furthermore, among within-participant longitudinal isolates of the same ST, the median pairwise SNP difference dropped to seven (IQR 2\u0026ndash;13). Additional analyses of the longitudinal MRSA and MSSA populations from NHR 201 revealed the MSSA strains isolated across all five collection times differed by a median of 25 (IQR 21.75\u0026ndash;29.75) SNPs (\u003cb\u003eFigure S5C\u003c/b\u003e), while the median pairwise SNP difference among the MRSA strains isolated across the other 10 collection times was 67 (IQR 25.5\u0026ndash;75.5) (\u003cb\u003eFigure S5D\u003c/b\u003e). Together, these data demonstrate that within-participant longitudinally collected isolates are a highly genetically related, single lineage that persists over time despite repeated catheter exchanges and anti-staphylococcal antibiotic exposures.\u003c/p\u003e\u003cp\u003e \u003cb\u003eAntimicrobial susceptibility profiles of\u003c/b\u003e \u003cb\u003eS. aureus\u003c/b\u003e \u003cb\u003eLTUC isolates\u003c/b\u003e\u003c/p\u003e \u003cp\u003eMost participants (12/20) received at least one antibiotic during the collection period (Fig.\u0026nbsp;\u003cspan refid=\"Fig1\" class=\"InternalRef\"\u003e1\u003c/span\u003e), and all isolates carried genes conferring resistance to multiple antibiotics (Fig.\u0026nbsp;\u003cspan refid=\"Fig5\" class=\"InternalRef\"\u003e5\u003c/span\u003e \u003cb\u003eand Table S6\u003c/b\u003e). Specifically, all isolates carried determinants that confer resistance to tetracyclines and Fosfomycin, and most encoded AMR genes associated with resistance to quinolones, macrolides, beta-lactams, and aminoglycosides. Only a few strains encoded AMR genes that confer resistance to lincosamide, rifamycin, mupirocin, bleomycin, and streptothricin. Importantly, the AMR determinant profiles were mostly stable among within-participant longitudinally collected isolates. Among the few isolates where AMR determinant instability was observed, these genes are associated with aminoglycoside resistance, which are generally carried on mobile genetic elements that are known to be readily gained and lost due to selective pressures. Additionally, a single instance of the loss of \u003cem\u003emecA-\u003c/em\u003eassociated genes was observed in an isolate from NHR 104 (104\u0026thinsp;\u0026minus;\u0026thinsp;21). SNP analysis revealed that the previously persisting MRSA strain and the subsequent MSSA strain differed by only one SNP (a missense mutation in a hypothetical protein), indicating this strain lost the \u003cem\u003emecA\u003c/em\u003e cassette (\u003cb\u003eTable S6\u003c/b\u003e).\u003c/p\u003e\u003cp\u003eNext, to determine the antimicrobial susceptibilities to the most commonly prescribed antibiotics for UTI, as well as some of the anti-staphylococcal antibiotics these participants received, minimum inhibitory concentration (MIC) assays were determined for index and final longitudinal isolates (Tables\u0026nbsp;\u003cspan refid=\"Tab2\" class=\"InternalRef\"\u003e2\u003c/span\u003e and S7). Additionally, NHR 104\u0026thinsp;\u0026minus;\u0026thinsp;21 was included due to notable changes in its AMR determinant profile, specifically the loss of \u003cem\u003emecA\u003c/em\u003e when compared to the index and other longitudinal isolates from NHR 104 (Fig.\u0026nbsp;\u003cspan refid=\"Fig5\" class=\"InternalRef\"\u003e5\u003c/span\u003e). NHR 201\u0026thinsp;\u0026minus;\u0026thinsp;11 was also selected based on similar changes; \u003cem\u003emecA\u003c/em\u003e was absent in the final isolate (NHR 201\u0026thinsp;\u0026minus;\u0026thinsp;17) compared to the index isolate (NHR 201-0), and NHR 201\u0026thinsp;\u0026minus;\u0026thinsp;11 was the last longitudinal isolate encoding \u003cem\u003emecA\u003c/em\u003e. Antibiotics assessed included nitrofurantoin, trimethoprim-sulfamethoxazole, ciprofloxacin, oxacillin, penicillin, and vancomycin. All isolates assessed were susceptible to nitrofurantoin, trimethoprim-sulfamethoxazole, and vancomycin. Most index and final isolates (10/13) retained the same antibiotic susceptibility pattern. Phenotypic non-susceptibility to oxacillin was generally concordant with genotype, with strains encoding \u003cem\u003emecA\u003c/em\u003e displaying resistance to oxacillin, and those that did not, including those that lost \u003cem\u003emecA\u003c/em\u003e (e.g. NHR 104\u0026thinsp;\u0026minus;\u0026thinsp;21), displaying susceptibility. However, both strains isolated from UP 129 encoded \u003cem\u003emecA\u003c/em\u003e, yet the strains were phenotypically susceptible to oxacillin and are therefore classified as MSSA. A missense mutation in \u003cem\u003emecA\u003c/em\u003e (T547C) was observed in both isolates, potentially explaining the susceptibility. Furthermore, in contrast to the index isolate, UP 129\u0026rsquo;s final isolate was susceptible to penicillin, which may be due to an additional missense mutation (C299T) in \u003cem\u003eblaZ\u003c/em\u003e. Together, these results demonstrate that there was concordance between genotypic and phenotypic antimicrobial susceptibility patterns of persistent \u003cem\u003eS. aureus\u003c/em\u003e LTUC isolates, which remained largely stable despite exposures to antimicrobials.\u003c/p\u003e\u003cp\u003e\u003cimg src=\"data:image/png;base64,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\" height=\"573\" width=\"488\"\u003e\u003c/p\u003e\u003cdiv id=\"Sec3\" class=\"Section2\"\u003e \u003ch2\u003eCarriage of AMR determinants and virulence factors is negatively correlated\u003c/h2\u003e \u003cp\u003eTo determine the virulence factor carriage profile of \u003cem\u003eS. aureus\u003c/em\u003e LTUC isolates, the virulence determinants encoded by all isolates were identified (\u003cb\u003eFigure S6 and Table S8\u003c/b\u003e). Similar to the AMR determinants, virulence gene profiles remained stable for most longitudinal isolates. Additional correlation analyses of the index isolates were used to determine whether there is a relationship between the number AMR determinants and virulence genes encoded by uropathogenic \u003cem\u003eS. aureus\u003c/em\u003e. A significant negative correlation was observed for the number of AMR determinants and virulence genes encoded (Fig.\u0026nbsp;\u003cspan refid=\"Fig6\" class=\"InternalRef\"\u003e6\u003c/span\u003e). Together, these data indicate that uropathogenic \u003cem\u003eS. aureus\u003c/em\u003e strains that carry more AMR genes encoded fewer virulence factors.\u003c/p\u003e\u003c/div\u003e"},{"header":"Discussion","content":"\u003cp\u003eUrinary catheterization is a common procedure that improves the quality of life for individuals with urinary tract dysfunction\u003csup\u003e\u003cspan citationid=\"CR4\" class=\"CitationRef\"\u003e4\u003c/span\u003e,\u003cspan citationid=\"CR5\" class=\"CitationRef\"\u003e5\u003c/span\u003e\u003c/sup\u003e. While this procedure is beneficial, urinary catheter duration is correlated with the risk of developing ASB and CAUTI, with longer dwell times associated with the highest risk\u003csup\u003e\u003cspan citationid=\"CR3\" class=\"CitationRef\"\u003e3\u003c/span\u003e,\u003cspan additionalcitationids=\"CR7\" citationid=\"CR6\" class=\"CitationRef\"\u003e6\u003c/span\u003e\u0026ndash;\u003cspan citationid=\"CR8\" class=\"CitationRef\"\u003e8\u003c/span\u003e,\u003cspan citationid=\"CR36\" class=\"CitationRef\"\u003e36\u003c/span\u003e\u003c/sup\u003e. Current treatment and prevention strategies include antimicrobial therapy and catheter exchanges\u003csup\u003e\u003cspan citationid=\"CR7\" class=\"CitationRef\"\u003e7\u003c/span\u003e,\u003cspan citationid=\"CR34\" class=\"CitationRef\"\u003e34\u003c/span\u003e\u003c/sup\u003e. Yet, recent studies demonstrate that prophylactic antibiotic administration does not prevent catheter colonization \u0026ndash; even during short catheter durations \u0026ndash; and may lead to more severe diseases, include \u003cem\u003eC. difficile\u003c/em\u003e colitis, as well as the selection of AMR\u003csup\u003e\u003cspan citationid=\"CR3\" class=\"CitationRef\"\u003e3\u003c/span\u003e,\u003cspan additionalcitationids=\"CR12 CR13\" citationid=\"CR11\" class=\"CitationRef\"\u003e11\u003c/span\u003e\u0026ndash;\u003cspan citationid=\"CR14\" class=\"CitationRef\"\u003e14\u003c/span\u003e\u003c/sup\u003e. Additionally, CAUTIs continue to be one of the most common hospital-associated infections in the US\u003csup\u003e\u003cspan citationid=\"CR28\" class=\"CitationRef\"\u003e28\u003c/span\u003e,\u003cspan citationid=\"CR37\" class=\"CitationRef\"\u003e37\u003c/span\u003e,\u003cspan citationid=\"CR38\" class=\"CitationRef\"\u003e38\u003c/span\u003e\u003c/sup\u003e.\u003c/p\u003e \u003cp\u003ePrevious studies indicate that the presence of \u003cem\u003eS. aureus\u003c/em\u003e in the urinary tract \u003cem\u003ei)\u003c/em\u003e is predominately associated with a urinary catheter, \u003cem\u003eii)\u003c/em\u003e is mainly MRSA, \u003cem\u003eiii)\u003c/em\u003e can increase reflux to the kidney by blocking catheters via urease-produced encrustations, and \u003cem\u003eiv)\u003c/em\u003e has a higher risk of disseminating to bacteremia compared to other uropathogens\u003csup\u003e\u003cspan citationid=\"CR9\" class=\"CitationRef\"\u003e9\u003c/span\u003e,\u003cspan citationid=\"CR12\" class=\"CitationRef\"\u003e12\u003c/span\u003e,\u003cspan additionalcitationids=\"CR30 CR31 CR32\" citationid=\"CR29\" class=\"CitationRef\"\u003e29\u003c/span\u003e\u0026ndash;\u003cspan citationid=\"CR33\" class=\"CitationRef\"\u003e33\u003c/span\u003e,\u003cspan citationid=\"CR39\" class=\"CitationRef\"\u003e39\u003c/span\u003e\u003c/sup\u003e. \u003cem\u003eS. aureus\u003c/em\u003e is also a frequent cause of ASB, and persistent \u003cem\u003eS. aureus\u003c/em\u003e ASB is associated with development of symptomatic infection\u003csup\u003e\u003cspan citationid=\"CR3\" class=\"CitationRef\"\u003e3\u003c/span\u003e,\u003cspan citationid=\"CR11\" class=\"CitationRef\"\u003e11\u003c/span\u003e,\u003cspan citationid=\"CR30\" class=\"CitationRef\"\u003e30\u003c/span\u003e\u003c/sup\u003e. Yet, \u003cem\u003eS. aureus\u003c/em\u003e remains largely uncharacterized during its lifecycle in the catheterized urinary tract. This study analyzed longitudinally collected \u003cem\u003eS. aureus\u003c/em\u003e strains isolated from two distinct cohorts: UPs and NHRs\u003csup\u003e\u003cspan citationid=\"CR3\" class=\"CitationRef\"\u003e3\u003c/span\u003e,\u003cspan citationid=\"CR11\" class=\"CitationRef\"\u003e11\u003c/span\u003e\u003c/sup\u003e. The demographics of both cohorts, including age, urinary catheter duration, and exposure to antibiotics, were similar. Whole-genome sequencing of all \u003cem\u003eS. aureus\u003c/em\u003e strains revealed ST5 as the most frequently isolated lineage among both cohorts. However, the second most common ST differed between cohorts: ST8 and ST45 among UPs and NHRs, respectively. ST5, ST8, and ST45 are among the most frequently circulating lineages causing infection within the US\u003csup\u003e\u003cspan additionalcitationids=\"CR41 CR42\" citationid=\"CR40\" class=\"CitationRef\"\u003e40\u003c/span\u003e\u0026ndash;\u003cspan citationid=\"CR43\" class=\"CitationRef\"\u003e43\u003c/span\u003e\u003c/sup\u003e. Additionally, recent studies indicate ST5 is a common lineage found within the urinary tract globally\u003csup\u003e\u003cspan citationid=\"CR44\" class=\"CitationRef\"\u003e44\u003c/span\u003e,\u003cspan citationid=\"CR45\" class=\"CitationRef\"\u003e45\u003c/span\u003e\u003c/sup\u003e. While most mechanistic studies of \u003cem\u003eS. aureus\u003c/em\u003e in the urinary tract assess ST8 isolates\u003csup\u003e\u003cspan citationid=\"CR29\" class=\"CitationRef\"\u003e29\u003c/span\u003e,\u003cspan citationid=\"CR45\" class=\"CitationRef\"\u003e45\u003c/span\u003e,\u003cspan citationid=\"CR46\" class=\"CitationRef\"\u003e46\u003c/span\u003e\u003c/sup\u003e, ST5 remains largely uncharacterized during CAUTI or ASB. Notably, the UP cohort was colonized with a wider diversity of STs compared to NHRs (eight vs four STs, respectively). This ST diversity may be due to the environmental differences within which UPs and NHRs reside\u003csup\u003e\u003cspan citationid=\"CR43\" class=\"CitationRef\"\u003e43\u003c/span\u003e,\u003cspan citationid=\"CR47\" class=\"CitationRef\"\u003e47\u003c/span\u003e,\u003cspan citationid=\"CR48\" class=\"CitationRef\"\u003e48\u003c/span\u003e\u003c/sup\u003e. NHRs live together in nursing homes, which may serve as a common environmental source of pathogen sharing. In contrast, UPs primarily reside at home within the community, resulting in potentially more diverse and participant-specific environmental sources of pathogen sharing.\u003c/p\u003e \u003cp\u003e\u003cem\u003eS. aureus\u003c/em\u003e was present at the first collection time point for 65% (13/20) of participants across both cohorts. Importantly, among within-participant longitudinal isolates, the same ST was stably detected over time despite regular catheter changes (~\u0026thinsp;27 days for UPs and ~\u0026thinsp;38 days for NHRs). There were only two instances \u0026ndash; one participant in each cohort (NHR 101 and UP 119) \u0026ndash; where the index isolate ST changed to a different ST at subsequent collections. NHR 101 was initially colonized with ST5; however, after a catheter exchange and trimethoprim-sulfamethoxazole administration, \u003cem\u003eS. aureus\u003c/em\u003e was no longer detected in the urine over the next 10 collections (10 weeks). At collection 19, an ST45 \u003cem\u003eS. aureus\u003c/em\u003e was cultured from the urine. Similarly, the index isolate collected for UP 119 was ST59. \u003cem\u003eS. aureus\u003c/em\u003e was not cultured from catheters over the next four collections (16 weeks), however, after ciprofloxacin treatment during collection four and five, an ST30 \u003cem\u003eS. aureus\u003c/em\u003e was cultured from the catheter at collection six. This ST30 lineage persisted despite regular, monthly catheter exchanges until the end of the study period. Notably, among the 35% (7/20) of participants that acquired \u003cem\u003eS. aureus\u003c/em\u003e during the collection period, all but one participant (NHR 204) received a catheter exchange or antibiotic exposure immediately prior to \u003cem\u003eS. aureus\u003c/em\u003e isolation. Together, these data suggest that catheter exchanges and antibiotic exposures may disrupt communities, eliminating other uropathogens, leaving a niche open for \u003cem\u003eS. aureus\u003c/em\u003e to rapidly recolonize. Additionally, while trimethoprim-sulfamethoxazole may have some efficacy at temporarily reducing \u003cem\u003eS. aureus\u003c/em\u003e bacterial burdens in the catheterized urinary tract, this contrasts with other anti-staphylococcal antibiotics, such as nitrofurantoin\u003csup\u003e\u003cspan citationid=\"CR3\" class=\"CitationRef\"\u003e3\u003c/span\u003e,\u003cspan citationid=\"CR11\" class=\"CitationRef\"\u003e11\u003c/span\u003e,\u003cspan citationid=\"CR49\" class=\"CitationRef\"\u003e49\u003c/span\u003e\u003c/sup\u003e, which had no effect on the persistence of UP 86\u0026rsquo;s \u003cem\u003eS. aureus\u003c/em\u003e isolates despite phenotypic susceptibility \u003cem\u003ein vitro\u003c/em\u003e.\u003c/p\u003e \u003cp\u003ePhylogenetic analyses of longitudinal \u003cem\u003eS. aureus\u003c/em\u003e strains demonstrate that within-participant isolates cluster more closely together compared to isolates from between participants, even among ST groups. Further genomic analyses indicated that within-participant isolates with the same ST were highly genetically related, with a median of four accessory genome changes and a median pairwise SNP difference of seven. For example, the 16 isolates from NHR 207 had a median of two accessory genome changes and a median pairwise SNP difference of seven. Additionally, among the four participants with co-isolates, genomic analyses demonstrated that these strains displayed a median of 2.5 accessory genome changes and a median pairwise SNP difference of five. Notably, while the MRSA and MSSA co-isolates from NHR 201 were from different lineages (72 accessory genome changes and SNP distance of 50) and the longitudinal MRSA strains were more heterogeneous (five accessory genome changes and SNP distance of 67), the longitudinal MSSA isolates were highly related to each other (three accessory genome changes and SNP distance of 25). NHR 201 had multiple antibiotic exposures over the course of the collection time, which may have provided additional pressures enabling more diverse lineage colonization. Interestingly, the MRSA strain colonized first, with the MSSA strain subsequently invading and eventually replacing the MRSA lineage. Taken together, these results are consistent with a previous study indicating a difference of 20 or fewer SNPs between strains can be used to identify a single, persisting \u003cem\u003eS. aureus\u003c/em\u003e strain\u003csup\u003e\u003cspan citationid=\"CR50\" class=\"CitationRef\"\u003e50\u003c/span\u003e\u003c/sup\u003e. Additionally, these data demonstrate that \u003cem\u003eS. aureus\u003c/em\u003e stably and asymptomatically persists within the catheterized bladder for up to nine months, resulting in persistent ASB. Importantly, most participants are dominated by a single homogeneous population that is highly genetically related, indicating a single lineage persists over time. This pattern was observed across geographically distinct sites (Midwest vs Northeast), suggesting these results are broadly applicable to individuals with LTUCs colonized with \u003cem\u003eS. aureus\u003c/em\u003e. Impactfully, the observed SNP increases are greater than the expected average accumulation of 2.72 SNPs per megabase per year observed when \u003cem\u003eS. aureus\u003c/em\u003e stably colonizes the nares\u003csup\u003e\u003cspan citationid=\"CR51\" class=\"CitationRef\"\u003e51\u003c/span\u003e\u003c/sup\u003e, indicating \u003cem\u003eS. aureus\u003c/em\u003e may be adapting to the urinary tract or exposure to antibiotics over time.\u003c/p\u003e \u003cp\u003eConsistent with previous reports\u003csup\u003e\u003cspan citationid=\"CR9\" class=\"CitationRef\"\u003e9\u003c/span\u003e,\u003cspan citationid=\"CR30\" class=\"CitationRef\"\u003e30\u003c/span\u003e\u003c/sup\u003e, half of the \u003cem\u003eS. aureus\u003c/em\u003e LTUC strains carried \u003cem\u003emecA\u003c/em\u003e and were resistant to oxacillin, making them MRSA. Additionally, most LTUC strains carry genes that confer resistance to a broad array of antibiotic classes, including fosfomycin and tetracyclines. Importantly, AMR gene carriage was stable across longitudinal isolates, despite most participants receiving at least one antibiotic during the study period and many receiving anti-staphylococcal antibiotics, including ciprofloxacin (5/20), trimethoprim-sulfamethoxazole (3/20), nitrofurantoin (3/20), and cephalexin (2/20)\u003csup\u003e3,11\u003c/sup\u003e. MIC assays using the antibiotics participants received or those that are commonly used to treat CAUTI demonstrated that antibiotic susceptibility patterns were stable among longitudinal isolates, with most strains (both index and final) displaying resistance to ciprofloxacin but susceptibility to nitrofurantoin. Despite treatment with antibiotics to which these isolates should be susceptible, the same strain was found to persist over the course of 12 months. For example, UP 86 received ciprofloxacin and nitrofurantoin over the course of the collection period\u003csup\u003e\u003cspan citationid=\"CR11\" class=\"CitationRef\"\u003e11\u003c/span\u003e\u003c/sup\u003e and the index and final isolates (collected 12 months apart) were intermediate resistant and susceptible, respectively.\u003c/p\u003e \u003cp\u003eOf the few strains that exhibited discordant antimicrobial susceptibility patterns between the index and final isolates, the most notable were the NHR 201 MRSA and MSSA isolates. However, the NHR 201 index and final MRSA isolates and the final MSSA isolate exhibited concordant genotypic and phenotypic beta-lactam susceptibility, with the differences due to invasion and replacement by a different lineage. Additionally, MRSA stably colonized NHR 104 for 26 weeks, yet \u003cem\u003emecA\u003c/em\u003e was lost at a single collection time (21), making it a MSSA strain. Since loss of \u003cem\u003emecA\u003c/em\u003e is rare, additional analyses performed determined that the MSSA and MRSA strains were the same ST and the strains collected at time point 0 (MRSA) and 21 (MSSA) differed by only one SNP, which occurred in a hypothetical protein. These results demonstrate that the MSSA and MRSA are the same strain, and that, while rare, \u003cem\u003emecA\u003c/em\u003e, and therefore resistance to beta-lactams, was lost during persistent \u003cem\u003eS. aureus\u003c/em\u003e ASB. In contrast to the index isolate, UP 129\u0026rsquo;s final isolate was also susceptible to penicillin likely due to a missense mutation (C299T) in \u003cem\u003eblaZ\u003c/em\u003e, a gene that encodes a beta-lactamase. MIC assays with oxacillin confirmed the predicted genotypic susceptibility of longitudinal isolates was generally concordant with phenotypic susceptibility. The only exceptions to this concordance were isolates from UP 129, which encoded the Scc\u003cem\u003emec\u003c/em\u003e Type IVc(2B), yet were phenotypically susceptible to oxacillin and therefore MSSA. A missense mutation in \u003cem\u003emecA\u003c/em\u003e (T547C) was observed in both isolates, potentially explaining the susceptibility. Together, these results highlight that LTUC isolates are multidrug resistant, further strengthening the hypothesis that ASB serves as a reservoir for drug resistant \u003cem\u003eS. aureus\u003c/em\u003e. In addition to AMR genes, virulence factor carriage was also stable among longitudinal isolates. Impactfully, carriage of AMR genes was inversely correlated with the presence of virulence factors: strains with increased AMR determinants encoded fewer virulence factors. Notably, the stable carriage of AMR genes across longitudinal \u003cem\u003eS. aureus\u003c/em\u003e LTUC isolates may help guide the selection of empiric antibiotics for treatment of subsequent CAUTI and therefore improve patient outcomes. Challengingly, these data also suggest that phenotypic resistance does not always correlate with \u003cem\u003eS. aureus\u003c/em\u003e persistence, as some strains that were susceptible to the antibiotics participants received during the collection period persisted as ASB. Thus, studies are needed to further dissect the \u003cem\u003eS. aureus\u003c/em\u003e recalcitrance mechanism that promote persistence.\u003c/p\u003e \u003cp\u003e This study was limited by the scope of both cohorts, which prioritized the isolation and sequencing of a single isolate per species for each participant at each collection. This collection strategy may limit the detection of heterogenous populations that may be present, as shown with NHR 201. Additionally, only the NHR cohort was designed to differentiate symptomatic CAUTI from ASB, which limited our ability to determine if strains persisting as ASB transitioned to cause symptomatic CAUTI in our UP cohort. Finally, while we used both long-and short-read sequencing for index isolates, only short-read sequencing was performed for longitudinal- and co-isolates. This approach allowed us to define the genomic synteny (long reads) and polish the genomes (short reads) of the index isolates to assess SNPs over time. However, without long-reads for the longitudinal and co-isolates, we may have missed large scale structural variations or gene gain/loss events that resulted from the movement or acquisition of mobile genetic elements. Notably, however, we were able to identify longitudinal and co-isolates with large accessory genome changes, limiting this concern. In depth analyses of these observations indicated two likely causes: 1) ST switches within participants suggest these changes were due to strain lineage differences, and 2) loss of large mobile genetic elements, including the \u003cem\u003emecA\u003c/em\u003e cassette, with few or no SNPs differences, indicate these are the same strain with gene loss. Nevertheless, most longitudinal and co-isolates were highly genetically related, and the pan-genome repertoire of each longitudinal set of isolates was stable, suggesting there are minimal longitudinal structural differences within most of the strain collection. Thus, most strains stably persist as ASB during long-term catheterization.\u003c/p\u003e \u003cp\u003eOur study demonstrates that a single \u003cem\u003eS. aureus\u003c/em\u003e strain persists over time despite antibiotic exposure or catheter exchanges, which are the two accepted practices for reducing microbial burdens during catheterization\u003csup\u003e\u003cspan citationid=\"CR7\" class=\"CitationRef\"\u003e7\u003c/span\u003e,\u003cspan citationid=\"CR34\" class=\"CitationRef\"\u003e34\u003c/span\u003e\u003c/sup\u003e. Importantly, these results are broadly applicable to LTUCs colonized with \u003cem\u003eS. aureus\u003c/em\u003e, as this pattern was observed across geographically distinct sites. Furthermore, while all strains tested are susceptible to nitrofurantoin, most strains (\u0026gt;\u0026thinsp;61%) are resistant to ciprofloxacin, which are two commonly prescribed antibiotics for the treatment of symptomatic CAUTI\u003csup\u003e\u003cspan citationid=\"CR7\" class=\"CitationRef\"\u003e7\u003c/span\u003e,\u003cspan citationid=\"CR11\" class=\"CitationRef\"\u003e11\u003c/span\u003e,\u003cspan citationid=\"CR28\" class=\"CitationRef\"\u003e28\u003c/span\u003e,\u003cspan citationid=\"CR52\" class=\"CitationRef\"\u003e52\u003c/span\u003e\u003c/sup\u003e. The ability of \u003cem\u003eS. aureus\u003c/em\u003e to persist despite susceptibility to the antibiotics participants received during the collection period warrants further investigation into the mechanisms driving this phenotypic recalcitrance. Importantly, the stable carriage of AMR determinants among longitudinal isolates suggests that monitoring AMR patterns of persistent \u003cem\u003eS. aureus\u003c/em\u003e ASB isolates may help guide physician selection of antibiotics to treat subsequent symptomatic CAUTI, which may improve the efficacy of empiric therapy and therefore patient outcomes. Finally, the accumulation of SNPs within longitudinal isolates suggest uropathogenic \u003cem\u003eS. aureus\u003c/em\u003e LTUC isolates are under selection. Future studies are needed to determine if and how \u003cem\u003eS. aureus\u003c/em\u003e is adapting to selective pressures, such as the urinary tract or antibiotics.\u003c/p\u003e"},{"header":"Methods","content":"\u003cp\u003e \u003cb\u003eStrains and growth conditions.\u003c/b\u003e \u003c/p\u003e \u003cp\u003eAll strains used in this study are from individuals with LTUCs from two separate, previously published cohorts and are listed in (\u003cb\u003eTable \u003cspan refid=\"MOESM1\" class=\"InternalRef\"\u003eS1\u003c/span\u003e\u003c/b\u003e)\u003csup\u003e\u003cspan citationid=\"CR3\" class=\"CitationRef\"\u003e3\u003c/span\u003e,\u003cspan citationid=\"CR11\" class=\"CitationRef\"\u003e11\u003c/span\u003e,\u003cspan citationid=\"CR12\" class=\"CitationRef\"\u003e12\u003c/span\u003e\u003c/sup\u003e. The first cohort is comprised of individuals seen at the Division of Urology at Washington University School of Medicine in St. Louis in Missouri\u003csup\u003e\u003cspan citationid=\"CR11\" class=\"CitationRef\"\u003e11\u003c/span\u003e,\u003cspan citationid=\"CR12\" class=\"CitationRef\"\u003e12\u003c/span\u003e\u003c/sup\u003e. Isolates were collected from rich media based on unique colony morphology and 16S sequencing confirmed the species. A total of 38 \u003cem\u003eS. aureus\u003c/em\u003e strains were collected from 2018\u0026ndash;2021, deidentified, and used as a part of this study. The second cohort is comprised of individuals residing in two separate nursing home facilities in New York\u003csup\u003e\u003cspan citationid=\"CR3\" class=\"CitationRef\"\u003e3\u003c/span\u003e\u003c/sup\u003e. Isolates were collected from selective media and differentiated by colony morphology with additional testing performed to identify species. A total of 133 \u003cem\u003eS. aureus\u003c/em\u003e strains were collected from 2019\u0026ndash;2020, deidentified, and used in this study. Mannitol Salt Agar (BD, cat 211407) plates were used to confirm and isolate all \u003cem\u003eS. aureus\u003c/em\u003e clinical isolates from both cohorts prior to whole-genome sequencing. Brain-heart infusion (BHI) broth (BD, cat. 237200) and agar (BD, cat. 214010) were used to maintain strains. Tryptic Soy Broth (TSB) (Sigma, cat. #22092-500G) was used to grow isolates for whole-genome sequencing. For MIC assays, bacterial isolates were grown in Mueller-Hinton broth (MHB) (Sigma; cat. 7019-100G) and MHB supplemented with 2% NaCl for oxacillin, according to the Clinical Laboratory Standard Institute (CLSI)\u003csup\u003e\u003cspan citationid=\"CR53\" class=\"CitationRef\"\u003e53\u003c/span\u003e\u003c/sup\u003e. Methicillin resistance using cefoxitin was previously reported for the NHR cohort\u003csup\u003e\u003cspan citationid=\"CR3\" class=\"CitationRef\"\u003e3\u003c/span\u003e\u003c/sup\u003e. Oxacillin resistance was confirmed for index and final isolates as described below.\u003c/p\u003e \u003cp\u003e \u003cb\u003eWhole-genome sequencing of the\u003c/b\u003e \u003cb\u003eS. aureus\u003c/b\u003e \u003cb\u003eisolates.\u003c/b\u003e\u003c/p\u003e \u003cp\u003eWhole-genome sequencing of all isolates (\u003cb\u003eTable \u003cspan refid=\"MOESM1\" class=\"InternalRef\"\u003eS1\u003c/span\u003e\u003c/b\u003e) was performed as previously described\u003csup\u003e\u003cspan citationid=\"CR54\" class=\"CitationRef\"\u003e54\u003c/span\u003e\u003c/sup\u003e. Briefly, \u003cem\u003eS. aureus\u003c/em\u003e strains were grown in TSB for 2\u0026ndash;4 h, shaking at 37\u0026deg;C. The cultures were pelleted and genomic DNA was extracted using the Qiagen QIAamp DNA minikit (Qiagen, cat. 51306) following the manufacturer's protocol, with the exception that 1 \u0026micro;g/mL of lysostaphin and 20 \u0026micro;g/mL of lysozyme was added to the initial buffer. Genomic DNA then underwent library preparation for short-read sequencing using the Illumina NextSeq 2000 sequencer. Genomes from short reads were assembled as previously described\u003csup\u003e\u003cspan citationid=\"CR54\" class=\"CitationRef\"\u003e54\u003c/span\u003e\u003c/sup\u003e. Briefly, read quality assessment was conducted with Raspberry v0.3, trimming was conducted with Trimmomatic v0.39, SPAdes v3.15.2 was used for assembly, and assembly quality was assessed using Quast v5.2.0\u003csup\u003e54\u003c/sup\u003e. Additional assembly quality control measures include BUSCO v5.7.0, for genome completeness, and CheckM v1.1.2, for genome quality control (\u003cb\u003eTables S2-3\u003c/b\u003e)\u003csup\u003e\u003cspan citationid=\"CR55\" class=\"CitationRef\"\u003e55\u003c/span\u003e,\u003cspan citationid=\"CR56\" class=\"CitationRef\"\u003e56\u003c/span\u003e\u003c/sup\u003e. Isolates were re-sequenced if the genome had\u0026thinsp;\u0026gt;\u0026thinsp;500 contigs, had a genome size greater or less than 2 standard deviations from the average of all \u003cem\u003eS. aureus\u003c/em\u003e genomes within NCBI, or if the assembly was contaminated with another bacterial species as defined by the presence of another bacterial species identified at greater than 2% by StrainSeeker v1.5\u003csup\u003e57\u003c/sup\u003e. Additionally, a subset of isolates was selected for Oxford Nanopore long-read sequencing to accurately reconstruct genome structures, including the circularization of plasmids and resolution of mobile genetic element integration. Isolates were chosen based on the identified ST from short-read sequencing, with the first isolate with a unique ST within a participant\u0026rsquo;s longitudinal set selected for long-read sequencing, which we define as an index isolate (\u003cb\u003eTable \u003cspan refid=\"MOESM1\" class=\"InternalRef\"\u003eS1\u003c/span\u003e\u003c/b\u003e). Briefly, the Oxford SQK-RBK004 library preparation kit was used to prepare bacterial DNA for sequencing on the Oxford Nanopore GridION X5 (Oxford, UK) using FLO-MIN106 flow cells. Genomes were assembled with Flye v2.9.1 and then underwent error correction and polishing steps using Racon v1.5.0 and Medaka to generate hybrid assemblies as previously described\u003csup\u003e\u003cspan citationid=\"CR58\" class=\"CitationRef\"\u003e58\u003c/span\u003e\u003c/sup\u003e.\u003c/p\u003e\n\u003ch3\u003eMolecular epidemiology of the bacterial assemblies\u003c/h3\u003e\n\u003cp\u003eMLST v2.19.0 was used to identify the ST for each isolate as previously described\u003csup\u003e\u003cspan citationid=\"CR54\" class=\"CitationRef\"\u003e54\u003c/span\u003e,\u003cspan citationid=\"CR59\" class=\"CitationRef\"\u003e59\u003c/span\u003e\u003c/sup\u003e. Gene annotations for each assembled genome were performed via Prokka v1.14.5 as previously described\u003csup\u003e\u003cspan citationid=\"CR54\" class=\"CitationRef\"\u003e54\u003c/span\u003e,\u003cspan citationid=\"CR60\" class=\"CitationRef\"\u003e60\u003c/span\u003e\u003c/sup\u003e.\u003c/p\u003e \u003cp\u003e \u003cb\u003ePan-genome and phylogenetic analysis of the\u003c/b\u003e \u003cb\u003eS. aureus\u003c/b\u003e \u003cb\u003eisolates\u003c/b\u003e\u003c/p\u003e \u003cp\u003eThe core genome for all isolates in the dataset, including index, co-isolates, and each set of longitudinal isolates for a participant, was identified with Roary v3.13.0 on the annotated short-read assemblies\u003csup\u003e\u003cspan citationid=\"CR61\" class=\"CitationRef\"\u003e61\u003c/span\u003e\u003c/sup\u003e. Accessory gene lists obtained from the Roary output were defined as genes present in \u0026gt;\u0026thinsp;99% of isolates. Additionally, to quantify the gene gain/loss events for each set of longitudinal and co-isolates from a participant, the accessory genome changes were identified by comparing the gene content of the index isolate to the longitudinal or co-isolated strains. Participants with single isolates were not included as no changes in gene content could be quantified. Final analysis and visualization were made in GraphPad Prism version 10.1.1. The core-gene alignment file from Roary was used with RAxML v8.2.12 to produce maximum-likelihood phylogenetic trees that were visualized using iTOL and edited on Affinity Designer as previously described\u003csup\u003e\u003cspan additionalcitationids=\"CR63\" citationid=\"CR62\" class=\"CitationRef\"\u003e62\u003c/span\u003e\u0026ndash;\u003cspan citationid=\"CR64\" class=\"CitationRef\"\u003e64\u003c/span\u003e\u003c/sup\u003e.\u003c/p\u003e\n\u003ch3\u003eSNPs analyses of clinical isolates\u003c/h3\u003e\n\u003cp\u003eSNP analyses were conducted using Snippy v4.6.0, aligning the paired-end reads from the queried isolate to the index isolate (\u003cb\u003eTable S9\u003c/b\u003e)\u003csup\u003e\u003cspan citationid=\"CR65\" class=\"CitationRef\"\u003e65\u003c/span\u003e\u003c/sup\u003e. For co-isolated strains, short-read assembled genomes were used to compare the index sequence to the sequences of the other co-isolated strains. To reduce potential bias in our longitudinal analyses, co-isolated strains of the same ST and \u003cem\u003emecA\u003c/em\u003e carriage as the index isolate were excluded as they had an average of less than 5 SNPs. In instances where two or more isolates were collected during the index collection, catheter isolates were prioritized. To identify genomic differences in our longitudinal cohort, the hybrid assembly of the index isolate from each set, defined as the first isolate of an ST within a participant, was used as a reference for each longitudinal strain to enable comprehensive assessment of whole-genome SNPs, including those in intergenic regions, and resolve the genomic synteny of these mutations. Self-self comparisons (i.e. comparing the index isolate\u0026rsquo;s short reads against the hybrid assembly) were excluded from statistical assessments as short-reads were used to polish the long-read assemblies to remove errors. Final analysis and visualization were made in GraphPad Prism version 10.1.1.\u003c/p\u003e \u003cdiv id=\"Sec8\" class=\"Section2\"\u003e \u003ch2\u003eAntimicrobial resistance and virulence factor profiles\u003c/h2\u003e \u003cp\u003eThe AMR profile of each strain was assessed using NCBI AMR Gene Finder Plus (AMRFinderPlus v3.11.8) for both mutational and acquired AMR mechanisms as well as species-specific virulence determinants (\u003cb\u003eTables S5-7\u003c/b\u003e), as previously described\u003csup\u003e\u003cspan citationid=\"CR66\" class=\"CitationRef\"\u003e66\u003c/span\u003e,\u003cspan citationid=\"CR67\" class=\"CitationRef\"\u003e67\u003c/span\u003e\u003c/sup\u003e. Virulence factors were obtained using Abricate (\u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003ehttps://github.com/tseemann/abricate\u003c/span\u003e\u003cspan address=\"https://github.com/tseemann/abricate\" targettype=\"URL\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e) using the virulence factor database (VFDB), as previously described\u003csup\u003e\u003cspan citationid=\"CR58\" class=\"CitationRef\"\u003e58\u003c/span\u003e,\u003cspan citationid=\"CR68\" class=\"CitationRef\"\u003e68\u003c/span\u003e\u003c/sup\u003e. This data set was prioritized for analyses as it encompassed a larger repertoire of determinants compared to AMRFinder Plus virulence determinants (\u003cb\u003eTable S8\u003c/b\u003e). Additionally, the \u003cem\u003emecA\u003c/em\u003e cassette type was identified using the Scc\u003cem\u003emec\u003c/em\u003e Finder 1.2 webtool\u003csup\u003e\u003cspan citationid=\"CR69\" class=\"CitationRef\"\u003e69\u003c/span\u003e\u003c/sup\u003e. The number of AMR determinants identified within the index isolate was compared to the number of virulence genes and a nonparametric Spearman\u0026rsquo;s correlation analysis was used to determine statistical correlation. Longitudinal isolates were excluded as most AMR and virulence profiles remained consistent, thus including them would violate the assumption of independence required for the statistical test.\u003c/p\u003e \u003c/div\u003e\n\u003ch3\u003eMinimum inhibitory concentration assays\u003c/h3\u003e\n\u003cp\u003eMICs were determined based on the 2024 CLSI guidelines as previously described\u003csup\u003e\u003cspan citationid=\"CR58\" class=\"CitationRef\"\u003e58\u003c/span\u003e\u003c/sup\u003e. Briefly, isolates were grown to ~\u0026thinsp;2.8E8 colony forming units (CFU)/mL in MHB or MHB supplemented with 2% NaCl for oxacillin per the CLSI guidelines, corresponding to an optical density at 600nm (OD\u003csub\u003e600\u003c/sub\u003e) of 0.4 using a Synergy H1 Biotek microtiter plate reader\u003csup\u003e\u003cspan citationid=\"CR53\" class=\"CitationRef\"\u003e53\u003c/span\u003e\u003c/sup\u003e. Cultures were then diluted to ~\u0026thinsp;1E6 CFU/mL and equal volumes of antibiotic and bacteria were transferred to a 96-well plate with final concentrations corresponding to the sensitive or resistant MIC breakpoints of each antibiotic tested and ~\u0026thinsp;5E5 CFU/mL. The final antibiotic concentrations used were as follows: 1, 2, and 4 \u0026micro;g/ml ciprofloxacin, 32 and 128 \u0026micro;g/ml nitrofurantoin, 2 and 4 \u0026micro;g/ml oxacillin, 0.125 and 0.25 \u0026micro;g/ml penicillin, 2/38 and 4/76 \u0026micro;g/ml trimethoprim-sulfamethoxine, and 1 and 16 \u0026micro;g/ml vancomycin. Plates were incubated at 37\u0026deg; or 35\u0026deg;C overnight following CLSI incubation times. Resistance was determined based on an OD\u003csub\u003e600\u003c/sub\u003e of greater than 0.1 and each condition consisted of at least 4 replicates \u0026ndash; 2 technical replicates and 2 biological replicates (\u003cb\u003eTable S9\u003c/b\u003e).\u003c/p\u003e \u003cdiv id=\"Sec10\" class=\"Section2\"\u003e \u003ch2\u003eStatistical Analysis\u003c/h2\u003e \u003cp\u003eStatistical significance between epidemiological descriptions was determined using the Fisher\u0026rsquo;s exact and the Mann-Whitney test. Significant differences for SNPs comparisons were determined using the Mann-Whitney U test. The Pearson\u0026rsquo;s correlation analysis was used to determine the correlation between number of antimicrobial resistance and virulence factors. All statistical tests were performed using GraphPad Prism version 10.1.1.\u003c/p\u003e \u003c/div\u003e \u003cdiv id=\"Sec11\" class=\"Section2\"\u003e \u003ch2\u003eData Availability\u003c/h2\u003e \u003cp\u003eAnnotated genomes were deposited into NCBI and are available under the BioProject ID: PRJNA1265249.\u003c/p\u003e \u003c/div\u003e"},{"header":"Declarations","content":"\u003cp\u003e \u003ch2\u003eRunning Title\u003c/h2\u003e \u003cp\u003e \u003cem\u003eS. aureus\u003c/em\u003e Persists during Urinary Catheterization\u003c/p\u003e \u003c/p\u003e\u003cp\u003e \u003ch2\u003eCompeting Interests\u003c/h2\u003e \u003cp\u003eThe authors declare no competing interests.\u003c/p\u003e \u003c/p\u003e\u003ch2\u003eAuthor Contributions\u003c/h2\u003e \u003cp\u003eJ.M.DR, C.E.A., B.M.H., and J.N.W. designed the research studies, J.M.DR, B.M.H., and J.N.W. performed the experiments, sequencing, data analyses, and drafted the manuscript, and all authors contributed to the editing of the manuscript.\u003c/p\u003e\u003ch2\u003eAcknowledgements\u003c/h2\u003e \u003cp\u003eThis work was supported by an NIH K01 DK128381-01A1 (J.N.W.), the University of Texas STAR award (JNW), an NIH K01 AI148593-01 (BMH), and an American Heart Association Predoctoral Fellowship (JMDR). We also thank Dr. Shelby R. Simar for scientific advice and guidance. Finally, we thank the study participants for their sample donations.\u003c/p\u003e"},{"header":"References","content":"\u003col\u003e\u003cli\u003e\u003cspan\u003eTrautner BW, Darouiche RO (2004) Catheter-associated infections: pathogenesis affects prevention. Arch Intern Med 164:842\u0026ndash;850. \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003e10.1001/archinte.164.8.842\u003c/span\u003e\u003cspan address=\"10.1001/archinte.164.8.842\" targettype=\"DOI\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eFlores-Mireles A, Hreha TN, Hunstad DA, Pathophysiology (2019) Treatment, and Prevention of Catheter-Associated Urinary Tract Infection. Top Spinal Cord Inj Rehabil 25:228\u0026ndash;240. \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003e10.1310/sci2503-228\u003c/span\u003e\u003cspan address=\"10.1310/sci2503-228\" targettype=\"DOI\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eArmbruster CE, Brauer AL, Humby MS, Shao J, Chakraborty S (2021) Prospective assessment of catheter-associated bacteriuria clinical presentation, epidemiology, and colonization dynamics in nursing home residents. JCI Insight 6. \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003e10.1172/jci.insight.144775\u003c/span\u003e\u003cspan address=\"10.1172/jci.insight.144775\" targettype=\"DOI\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eFumincelli L et al (2017) Quality of Life of Intermittent Urinary Catheterization Users and Their Caregivers: A Scoping Review. Worldviews Evid Based Nurs 14:324\u0026ndash;333. \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003e10.1111/wvn.12231\u003c/span\u003e\u003cspan address=\"10.1111/wvn.12231\" targettype=\"DOI\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003ePilloni S, Krhut J, Mair D, Madersbacher H, Kessler TM (2005) Intermittent catheterisation in older people: a valuable alternative to an indwelling catheter? Age Ageing 34:57\u0026ndash;60. \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003e10.1093/ageing/afh233\u003c/span\u003e\u003cspan address=\"10.1093/ageing/afh233\" targettype=\"DOI\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eNicolle LE (2014) Catheter associated urinary tract infections. Antimicrob Resist Infect Control 3:23. \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003e10.1186/2047-2994-3-23\u003c/span\u003e\u003cspan address=\"10.1186/2047-2994-3-23\" targettype=\"DOI\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eWerneburg GT (2022) Catheter-Associated Urinary Tract Infections: Current Challenges and Future Prospects. Res Rep Urol 14:109\u0026ndash;133. \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003e10.2147/rru.S273663\u003c/span\u003e\u003cspan address=\"10.2147/rru.S273663\" targettype=\"DOI\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eChenoweth C, Saint S (2013) Preventing catheter-associated urinary tract infections in the intensive care unit. Crit Care Clin 29:19\u0026ndash;32. \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003e10.1016/j.ccc.2012.10.005\u003c/span\u003e\u003cspan address=\"10.1016/j.ccc.2012.10.005\" targettype=\"DOI\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eShigemura K et al (2013) Clinical factors associated with shock in bacteremic UTI. Int Urol Nephrol 45:653\u0026ndash;657. \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003e10.1007/s11255-013-0449-4\u003c/span\u003e\u003cspan address=\"10.1007/s11255-013-0449-4\" targettype=\"DOI\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eMaffucci F, Chang C, Simhan J, Cohn JA (2023) Is There Any Benefit to the Use of Antibiotics with Indwelling Catheters after Urologic Surgery in Adults. Antibiot (Basel) 12. \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003e10.3390/antibiotics12010156\u003c/span\u003e\u003cspan address=\"10.3390/antibiotics12010156\" targettype=\"DOI\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eNye TM et al (2024) Microbial co-occurrences on catheters from long-term catheterized patients. Nat Commun 15:61. \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003e10.1038/s41467-023-44095-0\u003c/span\u003e\u003cspan address=\"10.1038/s41467-023-44095-0\" targettype=\"DOI\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eWalker JN et al (2020) High-resolution imaging reveals microbial biofilms on patient urinary catheters despite antibiotic administration. World J Urol 38:2237\u0026ndash;2245. \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003e10.1007/s00345-019-03027-8\u003c/span\u003e\u003cspan address=\"10.1007/s00345-019-03027-8\" targettype=\"DOI\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eCortes-Penfield NW, Trautner BW, Jump RLP (2017) Urinary Tract Infection and Asymptomatic Bacteriuria in Older Adults. Infect Dis Clin North Am 31:673\u0026ndash;688. \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003e10.1016/j.idc.2017.07.002\u003c/span\u003e\u003cspan address=\"10.1016/j.idc.2017.07.002\" targettype=\"DOI\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eCope M et al (2009) Inappropriate treatment of catheter-associated asymptomatic bacteriuria in a tertiary care hospital. Clin Infect Dis 48:1182\u0026ndash;1188. \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003e10.1086/597403\u003c/span\u003e\u003cspan address=\"10.1086/597403\" targettype=\"DOI\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eLiu F et al (2023) Prevalence and treatment of asymptomatic bacteriuria at academic and critical-access hospitals-Opportunities for stewardship efforts. Infect Control Hosp Epidemiol 44:979\u0026ndash;981. \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003e10.1017/ice.2022.143\u003c/span\u003e\u003cspan address=\"10.1017/ice.2022.143\" targettype=\"DOI\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eBabich T et al (2017) Empirical Antibiotic Treatment Does Not Improve Outcomes in Catheter-Associated Urinary Tract Infection: Prospective Cohort Study. Clin Infect Dis 65:1799\u0026ndash;1805. \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003e10.1093/cid/cix680\u003c/span\u003e\u003cspan address=\"10.1093/cid/cix680\" targettype=\"DOI\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eKlein RD, Hultgren SJ (2020) Urinary tract infections: microbial pathogenesis, host-pathogen interactions and new treatment strategies. Nat Rev Microbiol 18:211\u0026ndash;226. \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003e10.1038/s41579-020-0324-0\u003c/span\u003e\u003cspan address=\"10.1038/s41579-020-0324-0\" targettype=\"DOI\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eFlores-Mireles AL, Walker JN, Caparon M, Hultgren SJ (2015) Urinary tract infections: epidemiology, mechanisms of infection and treatment options. Nat Rev Microbiol 13:269\u0026ndash;284. \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003e10.1038/nrmicro3432\u003c/span\u003e\u003cspan address=\"10.1038/nrmicro3432\" targettype=\"DOI\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eGupta P et al (2023) Reducing catheter-associated urinary tract infections in the cardiac intensive care unit with a coordinated strategy and nursing staff empowerment. BMJ Open Qual 12. \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003e10.1136/bmjoq-2022-002214\u003c/span\u003e\u003cspan address=\"10.1136/bmjoq-2022-002214\" targettype=\"DOI\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eSaint S (2000) Clinical and economic consequences of nosocomial catheter-related bacteriuria. Am J Infect Control 28:68\u0026ndash;75. \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003e10.1016/s0196-6553(00)90015-4\u003c/span\u003e\u003cspan address=\"10.1016/s0196-6553(00)90015-4\" targettype=\"DOI\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003ePapanikolopoulou A et al (2022) Catheter-Associated Urinary Tract Infections, Bacteremia, and Infection Control Interventions in a Hospital: A Six-Year Time-Series Study. J Clin Med 11. \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003e10.3390/jcm11185418\u003c/span\u003e\u003cspan address=\"10.3390/jcm11185418\" targettype=\"DOI\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eGrillo S et al (2020) Characteristics and Outcomes of Staphylococcus aureus Bloodstream Infection Originating From the Urinary Tract: A Multicenter Cohort Study. Open Forum Infect Dis 7:ofaa216. \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003e10.1093/ofid/ofaa216\u003c/span\u003e\u003cspan address=\"10.1093/ofid/ofaa216\" targettype=\"DOI\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eGaston JR, Johnson AO, Bair KL, White AN, Armbruster CE (2021) Polymicrobial interactions in the urinary tract: is the enemy of my enemy my friend? Infect Immun. \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003e10.1128/iai.00652-20\u003c/span\u003e\u003cspan address=\"10.1128/iai.00652-20\" targettype=\"DOI\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eKline KA, Lewis AL, Gram-Positive Uropathogens (2016) Polymicrobial Urinary Tract Infection, and the Emerging Microbiota of the Urinary Tract. Microbiol Spectr 4. \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003e10.1128/microbiolspec.UTI-0012-2012\u003c/span\u003e\u003cspan address=\"10.1128/microbiolspec.UTI-0012-2012\" targettype=\"DOI\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eKu JH et al (2024) Antibiotic Resistance of Urinary Tract Infection Recurrences in a Large Integrated US Healthcare System. J Infect Dis 230:e1344\u0026ndash;e1354. \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003e10.1093/infdis/jiae233\u003c/span\u003e\u003cspan address=\"10.1093/infdis/jiae233\" targettype=\"DOI\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eMiller LG, Tang AW (2004) Treatment of uncomplicated urinary tract infections in an era of increasing antimicrobial resistance. Mayo Clin Proc 79:1048\u0026ndash;1053 quiz 1053\u0026thinsp;\u0026ndash;\u0026thinsp;1044. \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003e10.4065/79.8.1048\u003c/span\u003e\u003cspan address=\"10.4065/79.8.1048\" targettype=\"DOI\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eK\u0026ouml;ves B, Magyar A, Tenke P (2017) Spectrum and antibiotic resistance of catheter-associated urinary tract infections. GMS Infect Dis 5(Doc06). \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003e10.3205/id000032\u003c/span\u003e\u003cspan address=\"10.3205/id000032\" targettype=\"DOI\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eHooton TM et al (2010) Diagnosis, prevention, and treatment of catheter-associated urinary tract infection in adults: 2009 International Clinical Practice Guidelines from the Infectious Diseases Society of America. Clin Infect Dis 50:625\u0026ndash;663. \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003e10.1086/650482\u003c/span\u003e\u003cspan address=\"10.1086/650482\" targettype=\"DOI\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eWalker JN et al (2017) Catheterization alters bladder ecology to potentiate Staphylococcus aureus infection of the urinary tract. Proc Natl Acad Sci U S A 114:E8721\u0026ndash;e8730. \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003e10.1073/pnas.1707572114\u003c/span\u003e\u003cspan address=\"10.1073/pnas.1707572114\" targettype=\"DOI\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eMuder RR et al (2006) Isolation of Staphylococcus aureus from the urinary tract: association of isolation with symptomatic urinary tract infection and subsequent staphylococcal bacteremia. Clin Infect Dis 42:46\u0026ndash;50. \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003e10.1086/498518\u003c/span\u003e\u003cspan address=\"10.1086/498518\" targettype=\"DOI\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eKulbay A, Joelsson-Alm E, Amilon K, Tammelin A (2024) Asymptomatic bacteriuria and urinary tract infection in geriatric inpatients after indwelling urinary catheter removal: a descriptive two-centre study. Infect Prev Pract 6:100411. \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003e10.1016/j.infpip.2024.100411\u003c/span\u003e\u003cspan address=\"10.1016/j.infpip.2024.100411\" targettype=\"DOI\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eSaidel-Odes L, Riesenberg K, Schlaeffer F, Borer A (2009) Epidemiological and clinical characteristics of methicillin sensitive Staphylococcus aureus (MSSA) bacteriuria. J Infect 58:119\u0026ndash;122. \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003e10.1016/j.jinf.2008.11.014\u003c/span\u003e\u003cspan address=\"10.1016/j.jinf.2008.11.014\" targettype=\"DOI\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eHuggan PJ, Murdoch DR, Gallagher K, Chambers ST (2008) Concomitant Staphylococcus aureus bacteriuria is associated with poor clinical outcome in adults with S. aureus bacteraemia. J Hosp Infect 69:345\u0026ndash;349. \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003e10.1016/j.jhin.2008.04.027\u003c/span\u003e\u003cspan address=\"10.1016/j.jhin.2008.04.027\" targettype=\"DOI\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eWestgeest AC et al (2024) Catheter replacement in catheter-associated urinary tract infection: current state of evidence. Eur J Clin Microbiol Infect Dis 43:1631\u0026ndash;1637. \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003e10.1007/s10096-024-04878-9\u003c/span\u003e\u003cspan address=\"10.1007/s10096-024-04878-9\" targettype=\"DOI\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eRaghuram V et al (2023) Comparison of genomic diversity between single and pooled Staphylococcus aureus colonies isolated from human colonisation cultures. bioRxiv. \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003e10.1101/2023.06.14.544959\u003c/span\u003e\u003cspan address=\"10.1101/2023.06.14.544959\" targettype=\"DOI\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eMoon C, Wi YM (2025) Impact of antibiotic treatment and predictors for subsequent infections in multidrug-resistant Pseudomonas aeruginosa catheter-associated asymptomatic bacteriuria. Am J Infect Control. \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003e10.1016/j.ajic.2025.01.015\u003c/span\u003e\u003cspan address=\"10.1016/j.ajic.2025.01.015\" targettype=\"DOI\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eChenoweth CE, Gould CV, Saint S (2014) Diagnosis, management, and prevention of catheter-associated urinary tract infections. Infect Dis Clin North Am 28:105\u0026ndash;119. \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003e10.1016/j.idc.2013.09.002\u003c/span\u003e\u003cspan address=\"10.1016/j.idc.2013.09.002\" targettype=\"DOI\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eTrautner BW (2010) Management of catheter-associated urinary tract infection. Curr Opin Infect Dis 23:76\u0026ndash;82. \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003e10.1097/QCO.0b013e328334dda8\u003c/span\u003e\u003cspan address=\"10.1097/QCO.0b013e328334dda8\" targettype=\"DOI\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eStickler DJ (2014) Clinical complications of urinary catheters caused by crystalline biofilms: something needs to be done. J Intern Med 276:120\u0026ndash;129. \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003e10.1111/joim.12220\u003c/span\u003e\u003cspan address=\"10.1111/joim.12220\" targettype=\"DOI\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eLoftus RW et al (2024) Characterizing the molecular epidemiology of anaesthesia work area transmission of Staphylococcus aureus sequence type 5. J Hosp Infect 143:186\u0026ndash;194. \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003e10.1016/j.jhin.2023.07.003\u003c/span\u003e\u003cspan address=\"10.1016/j.jhin.2023.07.003\" targettype=\"DOI\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eHofstetter KS et al (2024) Strain Differences in Bloodstream and Skin Infection: Methicillin-Resistant Staphylococcus aureus Isolated in 2018\u0026ndash;2021 in a Single Health System. Open Forum Infect Dis 11:ofae261. \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003e10.1093/ofid/ofae261\u003c/span\u003e\u003cspan address=\"10.1093/ofid/ofae261\" targettype=\"DOI\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eHuang YC et al (2024) Dissemination of meticillin-resistant Staphylococcus aureus sequence type 8 (USA300) in Taiwan. J Hosp Infect 149:108\u0026ndash;118. \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003e10.1016/j.jhin.2024.04.024\u003c/span\u003e\u003cspan address=\"10.1016/j.jhin.2024.04.024\" targettype=\"DOI\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eTurner NA et al (2019) Methicillin-resistant Staphylococcus aureus: an overview of basic and clinical research. Nat Rev Microbiol 17:203\u0026ndash;218. \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003e10.1038/s41579-018-0147-4\u003c/span\u003e\u003cspan address=\"10.1038/s41579-018-0147-4\" targettype=\"DOI\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eXu K et al (2023) Staphylococcus aureus ST1 promotes persistent urinary tract infection by highly expressing the urease. Front Microbiol 14:1101754. \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003e10.3389/fmicb.2023.1101754\u003c/span\u003e\u003cspan address=\"10.3389/fmicb.2023.1101754\" targettype=\"DOI\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eSaenkham-Huntsinger P et al (2021) Copper Resistance Promotes Fitness of Methicillin-Resistant Staphylococcus aureus during Urinary Tract Infection. \u003cem\u003emBio\u003c/em\u003e 12, e0203821. \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003e10.1128/mBio.02038-21\u003c/span\u003e\u003cspan address=\"10.1128/mBio.02038-21\" targettype=\"DOI\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003ePaudel S, Bagale K, Patel S, Kooyers NJ, Kulkarni R (2021) Human Urine Alters Methicillin-Resistant Staphylococcus aureus Virulence and Transcriptome. Appl Environ Microbiol 87:e0074421. \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003e10.1128/aem.00744-21\u003c/span\u003e\u003cspan address=\"10.1128/aem.00744-21\" targettype=\"DOI\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eMustapha MM et al (2022) Genomic Diversity of Hospital-Acquired Infections Revealed through Prospective Whole-Genome Sequencing-Based Surveillance. \u003cem\u003emSystems\u003c/em\u003e 7, e0138421. \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003e10.1128/msystems.01384-21\u003c/span\u003e\u003cspan address=\"10.1128/msystems.01384-21\" targettype=\"DOI\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eCespedes C et al (2002) Differences between Staphylococcus aureus isolates from medical and nonmedical hospital personnel. J Clin Microbiol 40:2594\u0026ndash;2597. \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003e10.1128/jcm.40.7.2594-2597.2002\u003c/span\u003e\u003cspan address=\"10.1128/jcm.40.7.2594-2597.2002\" targettype=\"DOI\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eAlshomrani MK, Alharbi AA, Alshehri AA, Arshad M, Dolgum S (2023) Isolation of Staphylococcus aureus Urinary Tract Infections at a Community-Based Healthcare Center in Riyadh. Cureus 15:e35140. \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003e10.7759/cureus.35140\u003c/span\u003e\u003cspan address=\"10.7759/cureus.35140\" targettype=\"DOI\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eGoyal M et al (2019) Genomic Evolution of Staphylococcus aureus During Artificial and Natural Colonization of the Human Nose. Front Microbiol 10:1525. \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003e10.3389/fmicb.2019.01525\u003c/span\u003e\u003cspan address=\"10.3389/fmicb.2019.01525\" targettype=\"DOI\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eYoung BC et al (2012) Evolutionary dynamics of Staphylococcus aureus during progression from carriage to disease. Proc Natl Acad Sci U S A 109:4550\u0026ndash;4555. \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003e10.1073/pnas.1113219109\u003c/span\u003e\u003cspan address=\"10.1073/pnas.1113219109\" targettype=\"DOI\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eKaur K et al (2014) Evaluation of efficacy and tolerability of cefotaxime and sulbactam versus cefepime and tazobactam in patients of urinary tract infection-a prospective comparative study. J Clin Diagn Res 8:Hc05\u0026ndash;08. \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003e10.7860/jcdr/2014/9742.5090\u003c/span\u003e\u003cspan address=\"10.7860/jcdr/2014/9742.5090\" targettype=\"DOI\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eWayne P (2017) CLSI Performance Standards for Antimicrobial Susceptibility Testing. \u003cem\u003eCLSI Document Clinical Laboratory Standards Institute (CLSI): Wayne, PA, USA\u003c/em\u003e\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003evan Duin D et al (2020) Molecular and clinical epidemiology of carbapenem-resistant Enterobacterales in the USA (CRACKLE-2): a prospective cohort study. Lancet Infect Dis 20:731\u0026ndash;741. \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003e10.1016/s1473-3099(19)30755-8\u003c/span\u003e\u003cspan address=\"10.1016/s1473-3099(19)30755-8\" targettype=\"DOI\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eManni M, Berkeley MR, Seppey M, Sim\u0026atilde;o FA, Zdobnov EM (2021) BUSCO Update: Novel and Streamlined Workflows along with Broader and Deeper Phylogenetic Coverage for Scoring of Eukaryotic, Prokaryotic, and Viral Genomes. Mol Biol Evol 38:4647\u0026ndash;4654. \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003e10.1093/molbev/msab199\u003c/span\u003e\u003cspan address=\"10.1093/molbev/msab199\" targettype=\"DOI\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eParks DH, Imelfort M, Skennerton CT, Hugenholtz P, Tyson GW (2015) CheckM: assessing the quality of microbial genomes recovered from isolates, single cells, and metagenomes. Genome Res 25:1043\u0026ndash;1055. \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003e10.1101/gr.186072.114\u003c/span\u003e\u003cspan address=\"10.1101/gr.186072.114\" targettype=\"DOI\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eRoosaare M et al (2017) StrainSeeker: fast identification of bacterial strains from raw sequencing reads using user-provided guide trees. PeerJ 5:e3353. \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003e10.7717/peerj.3353\u003c/span\u003e\u003cspan address=\"10.7717/peerj.3353\" targettype=\"DOI\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eDuran Ramirez JM et al (2023) Staphylococcus aureus Breast Implant Infection Isolates Display Recalcitrance To Antibiotic Pocket Irrigants. Microbiol Spectr 11:e0288422. \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003e10.1128/spectrum.02884-22\u003c/span\u003e\u003cspan address=\"10.1128/spectrum.02884-22\" targettype=\"DOI\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eJolley KA, Maiden MC, BIGSdb (2010) Scalable analysis of bacterial genome variation at the population level. BMC Bioinformatics 11:595. \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003e10.1186/1471-2105-11-595\u003c/span\u003e\u003cspan address=\"10.1186/1471-2105-11-595\" targettype=\"DOI\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eSeemann T (2014) Prokka: rapid prokaryotic genome annotation. Bioinformatics 30:2068\u0026ndash;2069. \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003e10.1093/bioinformatics/btu153\u003c/span\u003e\u003cspan address=\"10.1093/bioinformatics/btu153\" targettype=\"DOI\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003ePage AJ et al (2015) Roary: rapid large-scale prokaryote pan genome analysis. Bioinformatics 31:3691\u0026ndash;3693. \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003e10.1093/bioinformatics/btv421\u003c/span\u003e\u003cspan address=\"10.1093/bioinformatics/btv421\" targettype=\"DOI\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eContreras GA et al (2022) Contemporary Clinical and Molecular Epidemiology of Vancomycin-Resistant Enterococcal Bacteremia: A Prospective Multicenter Cohort Study (VENOUS I). Open Forum Infect Dis 9:ofab616. \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003e10.1093/ofid/ofab616\u003c/span\u003e\u003cspan address=\"10.1093/ofid/ofab616\" targettype=\"DOI\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eStamatakis A (2014) RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics 30:1312\u0026ndash;1313. \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003e10.1093/bioinformatics/btu033\u003c/span\u003e\u003cspan address=\"10.1093/bioinformatics/btu033\" targettype=\"DOI\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eLetunic I, Bork P (2019) Interactive Tree Of Life (iTOL) v4: recent updates and new developments. Nucleic Acids Res 47:W256\u0026ndash;w259. \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003e10.1093/nar/gkz239\u003c/span\u003e\u003cspan address=\"10.1093/nar/gkz239\" targettype=\"DOI\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eSeemann T (2015) Snippy: rapid haploid variant calling and core SNP phylogeny. \u003cem\u003eGitHub. Available at: github. com/tseemann/snippy\u003c/em\u003e\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eFeldgarden M et al (2021) AMRFinderPlus and the Reference Gene Catalog facilitate examination of the genomic links among antimicrobial resistance, stress response, and virulence. Sci Rep 11:12728. \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003e10.1038/s41598-021-91456-0\u003c/span\u003e\u003cspan address=\"10.1038/s41598-021-91456-0\" targettype=\"DOI\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eSakurai A et al (2023) Evolving landscape of carbapenem-resistant Pseudomonas aeruginosa at a single centre in the USA. JAC Antimicrob Resist 5:dlad070. \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003e10.1093/jacamr/dlad070\u003c/span\u003e\u003cspan address=\"10.1093/jacamr/dlad070\" targettype=\"DOI\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eSeemann T (2016) ABRicate: mass screening of contigs for antibiotic resistance genes. San Francisco CA: GitHub\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eKaya H et al (2018) SCCmecFinder, a Web-Based Tool for Typing of Staphylococcal Cassette Chromosome mec in Staphylococcus aureus Using Whole-Genome Sequence Data. \u003cem\u003emSphere\u003c/em\u003e 3. \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003e10.1128/mSphere.00612-17\u003c/span\u003e\u003cspan address=\"10.1128/mSphere.00612-17\" targettype=\"DOI\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/li\u003e\u003c/ol\u003e"}],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":true,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":true,"hideJournal":false,"highlight":"","institution":"","isAcceptedByJournal":true,"isAuthorSuppliedPdf":false,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":false,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"nature-portfolio","isNatureJournal":true,"hasQc":false,"allowDirectSubmit":false,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"","title":"Nature Portfolio","twitterHandle":"","acdcEnabled":false,"dfaEnabled":false,"editorialSystem":"ejp","reportingPortfolio":"","inReviewEnabled":true,"inReviewRevisionsEnabled":false},"keywords":"CAUTI, asymptomatic bacteriuria, Staphylococcus aureus persistence, long-term human urinary catheterization","lastPublishedDoi":"10.21203/rs.3.rs-6702271/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-6702271/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003eUrinary catheters, the most frequently placed medical devices in the US, increase the risk of developing symptomatic catheter-associated urinary tract infection (CAUTI) and asymptomatic bacteriuria (ASB) \u0026ndash; the presence of bacteria in the urine \u0026ndash; with those requiring long-term urinary catheters (LTUCs) at highest risk. While ASB and CAUTI are caused by a broad range of uropathogens, most remain understudied. We used whole-genome sequencing to investigate the understudied uropathogen, \u003cem\u003eStaphylococcus aureus\u003c/em\u003e. Analysis of 153 longitudinal \u003cem\u003eS. aureus\u003c/em\u003e isolates previously collected from urinary catheter or urine samples from 20 individuals with LTUCs (average of 8 longitudinal isolates/person) demonstrated that most strains were multidrug resistant (MDR), including methicillin-resistant \u003cem\u003eS. aureus\u003c/em\u003e (MRSA), and sequence type 5. Importantly, the same \u003cem\u003eS. aureus\u003c/em\u003e strain persisted as ASB for an average of 13 weeks, despite antibiotic exposures or urinary catheter exchanges, and in one case transitioned to symptomatic CAUTI. The longitudinal strains were highly genetically related with few genomic changes and stable antimicrobial resistance and virulence gene carriage. This work demonstrates that MDR \u003cem\u003eS. aureus\u003c/em\u003e can persist as ASB long-term and that common strategies to reduce or eliminate microbes from LTUCs were ineffective at eradicating the uropathogen in most cases, despite phenotypic susceptibility to the administered antibiotic.\u003c/p\u003e","manuscriptTitle":"Genomics Reveal Staphylococcus aureus Persists during Long-term Urinary Catheterization Despite Antimicrobial Therapy and Catheter Exchanges","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2025-05-29 12:39:12","doi":"10.21203/rs.3.rs-6702271/v1","editorialEvents":[],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"nature-communications","isNatureJournal":true,"hasQc":false,"allowDirectSubmit":false,"externalIdentity":"NCOMMS","sideBox":"Learn more about [Nature Communications](http://www.nature.com/ncomms/)","snPcode":"","submissionUrl":"https://mts-ncomms.nature.com/","title":"Nature Communications","twitterHandle":"","acdcEnabled":true,"dfaEnabled":true,"editorialSystem":"ejp","reportingPortfolio":"Nature Communications","inReviewEnabled":true,"inReviewRevisionsEnabled":false}}],"origin":"","ownerIdentity":"42d0cf81-2da6-4cb1-848d-0bf6d272955a","owner":[],"postedDate":"May 29th, 2025","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"published-in-journal","subjectAreas":[{"id":49159329,"name":"Health sciences/Urology/Urogenital diseases/Urinary tract infection"},{"id":49159330,"name":"Biological sciences/Microbiology/Bacteriology"}],"tags":[],"updatedAt":"2026-02-05T08:14:11+00:00","versionOfRecord":{"articleIdentity":"rs-6702271","link":"https://doi.org/10.1038/s41467-025-68081-w","journal":{"identity":"nature-communications","isVorOnly":false,"title":"Nature Communications"},"publishedOn":"2026-01-13 05:00:00","publishedOnDateReadable":"January 13th, 2026"},"versionCreatedAt":"2025-05-29 12:39:12","video":"","vorDoi":"10.1038/s41467-025-68081-w","vorDoiUrl":"https://doi.org/10.1038/s41467-025-68081-w","workflowStages":[]},"version":"v1","identity":"rs-6702271","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-6702271","identity":"rs-6702271","version":["v1"]},"buildId":"8U1c8b4HqxoKbykW_rLl7","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}