The Curious Case of CysE: Diversity and Distribution of Serine Acetyltransferases in Bacteria

The Curious Case of CysE: Diversity and Distribution of Serine Acetyltransferases in Bacteria | Authorea try { document.documentElement.classList.add('js'); } catch (e) { } var _gaq = _gaq || []; _gaq.push(['_setAccount', 'G-8VDV14Y67G']); _gaq.push(['_trackPageview']); (function() { var ga = document.createElement('script'); ga.type = 'text/javascript'; ga.async = true; ga.src = ('https:' == document.location.protocol ? 'https://ssl' : 'http://www') + '.google-analytics.com/ga.js'; var s = document.getElementsByTagName('script')[0]; s.parentNode.insertBefore(ga, s); })(); Skip to main content Preprints Collections Wiley Open Research IET Open Research Ecological Society of Japan All Collections About About Authorea FAQs Contact Us Quick Search anywhere Search for preprint articles, keywords, etc. Search Search ADVANCED SEARCH SCROLL PROTEINS: Structure, Function, and Bioinformatics This is a preprint and has not been peer reviewed. Data may be preliminary. 27 June 2025 V1 Latest version Share on The Curious Case of CysE: Diversity and Distribution of Serine Acetyltransferases in Bacteria Authors : Keely E.A. Oldham , Adele K. Williamson , Emily K. Grout , and Joanna Hicks 0000-0002-0307-0923 [email protected] Authors Info & Affiliations https://doi.org/10.22541/au.175101679.91384522/v1 Published Proteins: Structure, Function, and Bioinformatics Version of record Peer review timeline 253 views 152 downloads Contents Abstract Supplementary Material Information & Authors Metrics & Citations View Options References Figures Tables Media Share Abstract Serine acetyltransferase (CysE) is a member of the left-handed β-helix family of acetyltransferases that catalyse the rate limiting step in de novo cysteine biosynthesis. There are two isoforms of CysE that differ in length, with the shorter isoform lacking approximately 76 amino acids at the N-terminus of the protein from the serine acetyltransferase (SATase) domain. Here, we analyse the distribution and diversity of CysE isoforms across the bacterial kingdom. The isoforms can be classified into two discrete groups, with the truncated isoform prevalent in Gram positive bacteria and the full-length isoform dominant in Proteobacteria. Moreover, we demonstrate that the truncation is discrete with the loss of four N-terminal α-helices conserved for the truncated isoform. Using predictive modelling we show that this truncation likely weakens the CysE trimer-trimer interface potentially resulting in a trimeric assembly instead of the canonical CysE hexamer. This expands our understanding of CysE enzymes and their distribution across bacterial species, an important consideration given the increasing interest in targeting CysE enzymes for developing potential antimicrobials. Supplementary Material File (cyse_phylo_manuscript_final.docx) Download 6.35 MB Information & Authors Information Version history V1 Version 1 27 June 2025 Peer review timeline Published Proteins: Structure, Function, and Bioinformatics Version of Record 16 Dec 2025 Published Copyright This work is licensed under a Non Exclusive No Reuse License. Collection PROTEINS: Structure, Function, and Bioinformatics Keywords cyse cysteine biosynthesis hexapeptide repeat l-cysteine n-terminal truncation sat serine acetyltransferase Authors Affiliations Keely E.A. Oldham The University of Waikato View all articles by this author Adele K. Williamson The University of Waikato School of Science View all articles by this author Emily K. Grout The University of Waikato View all articles by this author Joanna Hicks 0000-0002-0307-0923 [email protected] The University of Waikato View all articles by this author Metrics & Citations Metrics Article Usage 253 views 152 downloads .FvxKWukQNSOunydq8rnd { width: 100px; } Citations Download citation Keely E.A. Oldham, Adele K. Williamson, Emily K. Grout, et al. The Curious Case of CysE: Diversity and Distribution of Serine Acetyltransferases in Bacteria. Authorea . 27 June 2025. DOI: https://doi.org/10.22541/au.175101679.91384522/v1 If you have the appropriate software installed, you can download article citation data to the citation manager of your choice. Simply select your manager software from the list below and click Download. For more information or tips please see 'Downloading to a citation manager' in the Help menu . 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