Arsenate reduction coupled to anaerobic oxidation of methane by members of the Methanoperedenaceae

Arsenate reduction coupled to anaerobic oxidation of methane by members of the Methanoperedenaceae | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Article Arsenate reduction coupled to anaerobic oxidation of methane by members of the Methanoperedenaceae Simon McIlroy, Georgina Joyce, Xueqin Zhang, Andy Leu, Yosephine Gumulya, and 3 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-9109145/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Anaerobic methanotrophic ‘Candidatus Methanoperedenaceae’ play a key role mitigating methane emissions from freshwater sediments. Members of the family are metabolically versatile, with species coupling anaerobic oxidation of methane (AOM) to the reduction of nitrate, metal oxides and/or humic compounds. Metagenomic analyses have indicated further uncharacterised metabolic diversity, including the potential for AOM coupled to arsenate reduction (As-AOM). While As-AOM has been confirmed as an important methane sink and contributor to arsenic mobilisation in freshwater reservoirs, the underlying microbiological mechanisms remain to be empirically demonstrated. In this study stoichiometric coupling of dissimilatory arsenate reduction to methane oxidation was demonstrated using enrichment cultures of the uncultured type species ‘Ca. Methanoperedens nitroreducens’. Meta-omics revealed that arsenate oxidoreductases were exclusively encoded by ‘Ca. M. nitroreducens’ and upregulated under As-AOM conditions, confirming this species was directly responsible. These findings expand our understanding of ‘Ca. Methanoperedenaceae’ physiology and demonstrate microbial mechanisms underpinning arsenic mobilisation in freshwater systems, with important implications for climate and public health. Biological sciences/Microbiology/Environmental microbiology/Soil microbiology Biological sciences/Microbiology/Microbial communities/Microbial ecology Biological sciences/Microbiology/Microbial communities/Metagenomics Full Text Additional Declarations There is NO Competing Interest. Supplementary Files Joycesuppfigs.pdf Supplementary Figures Joycesupplementarydatasets.xlsx Supplementary Datasets Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-9109145","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Article","associatedPublications":[],"authors":[{"id":605754557,"identity":"9bfe0ccd-c1da-4f32-8f02-0b9db43ac683","order_by":0,"name":"Simon 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