Mitochondrial Phosphate Carbokinase Inhibits Renal Cell Carcinoma Malignant Progression, Leading to Cell Energy Metabolism Imbalance

Mitochondrial phosphoenolpyruvate carboxykinase (PCK2) is a key gluconeogenesis enzyme. Its differential expression is related to renal cell carcinoma (RCC) malignancy, possibly by influencing energy metabolism.We found that, compared with 293t, PCK2 was downregulated in six RCC lines. Relative to surrounding tissues, PCK2 was downregulated in RCC. PCK2 overexpression inhibited RCC proliferation. PCK2 overexpression inhibited RCC migration and invasion. Mass spectrometry revealed that thiamine pyrophosphate, cyclic AMP, beta-D-Fructose 6-phosphate, lactate, flavin mononucleotide, NAD, NADP, D-Glucose 6-phosphate were upregulated. In conclusion, our results showed that PCK2 overexpression inhibited RCC proliferation, migration, and invasion and upregulated energy metabolite expression.