The Evaluation of the efficacy evaluation of FDG-PET/CT forin assessing the BMMSCs improvementeffects of BMMSC treatment in aged rhesus monkeys

The Evaluation of the efficacy evaluation of FDG-PET/CT forin assessing the BMMSCs improvementeffects of BMMSC treatment in aged rhesus monkeys | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article The Evaluation of the efficacy evaluation of FDG-PET/CT forin assessing the BMMSCs improvementeffects of BMMSC treatment in aged rhesus monkeys Yaohui Zhang, Jie Li, Longbao Lü, Yuanyuan li, Zhiyu Yang, Shici Yang, and 4 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-5616853/v1 This work is licensed under a CC BY 4.0 License Status: Published Journal Publication published 22 Aug, 2025 Read the published version in Stem Cell Research & Therapy → Version 1 posted 5 You are reading this latest preprint version Abstract To evaluate the therapeutic efficacy of BMMSCs in aged rhesus monkeys, histological texture features were analyzed using 18 F-FDG-PET/CT imaging. The study involved three groups: five juvenile rhesus monkeys (mean age 2 years, weight 4.6 ± 0.49 kg), five young adult rhesus monkeys (mean age 6.2 ± 0.40 years, weight 3.6 ± 0.80 kg), and six aged rhesus monkeys (mean age 23.3 ± 1.37 years, weight 7.0 ± 2.77 kg). The aged group underwent 18 F -FDG-PET/CT imaging at baseline, and again at 3 and 6 months following BMMSC administration. Image texture features were segmented, extracted, and analyzed using LIFEx software, with a total of 93 high-throughput PET texture features evaluated across the adolescent, young adult, and aged groups, as well as at the 3- and 6-month post-treatment time points.Significant differences (P < 0.05) in 82 texture features were observed in multiple organs, including the brain, bilateral retroocular fat, thyroid, thymus, lung, aorta, gastric sinusoids, liver, kidneys, and L4 vertebrae, across the three age groups. Comparison between the younger and older groups revealed significant differences (P < 0.05) in 31 texture features within the brain, bilateral retroocular fat, thyroid, lung, gastric sinus, left kidney, and L4 vertebrae.ROC curve analysis of the PET texture features from the thyroid, lung, gastric sinus, left kidney, and L4 vertebrae showed AUC values greater than 0.7, indicating good diagnostic performance. In aged rhesus monkeys treated with BMMSCs, PET texture features in the brain, bilateral retroocular fat, left lung, gastric sinus, and L4 vertebrae recovered to levels comparable to those of the young group at both the 3- and 6-month post-treatment time points. Additionally, histological examination via HE staining of the lung, liver, kidney, stomach, and thymus in the aged group at 6 months post-treatment revealed significant improvements in the structural integrity of these organs.These findings suggest that 18 F -FDG PET texture analysis is a valuable tool for assessing the restoration of physiological function in aged rhesus monkeys following BMMSC treatment. Aged rhesus monkeys BMMSCs 18F-FDG PET Texture Multi-organ studies Full Text Cite Share Download PDF Status: Published Journal Publication published 22 Aug, 2025 Read the published version in Stem Cell Research & Therapy → Version 1 posted Reviewers agreed at journal 25 Apr, 2025 Reviewers invited by journal 24 Apr, 2025 Editor assigned by journal 23 Apr, 2025 First submitted to journal 22 Apr, 2025 Editorial decision: Minor Revision 17 Dec, 2024 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-5616853","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":447323022,"identity":"67c49986-7c94-4126-9d16-7f4537d0771b","order_by":0,"name":"Yaohui Zhang","email":"","orcid":"","institution":"Kunming Medical University First Affiliated Hospital: First Affiliated Hospital of Kunming Medical University","correspondingAuthor":false,"prefix":"","firstName":"Yaohui","middleName":"","lastName":"Zhang","suffix":""},{"id":447323023,"identity":"c41988f0-26d1-44d1-97ec-4a888ef93009","order_by":1,"name":"Jie 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The study involved three groups: five juvenile rhesus monkeys (mean age 2 years, weight 4.6\u0026thinsp;\u0026plusmn;\u0026thinsp;0.49 kg), five young adult rhesus monkeys (mean age 6.2\u0026thinsp;\u0026plusmn;\u0026thinsp;0.40 years, weight 3.6\u0026thinsp;\u0026plusmn;\u0026thinsp;0.80 kg), and six aged rhesus monkeys (mean age 23.3\u0026thinsp;\u0026plusmn;\u0026thinsp;1.37 years, weight 7.0\u0026thinsp;\u0026plusmn;\u0026thinsp;2.77 kg). The aged group underwent \u003csup\u003e18\u003c/sup\u003eF -FDG-PET/CT imaging at baseline, and again at 3 and 6 months following BMMSC administration. 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