Molecular characterization of a novel ourmia‐like virus infecting the plant-pathogenic fungus Botryosphaeria dothidea

Molecular characterization of a novel ourmia‐like virus infecting the plant-pathogenic fungus Botryosphaeria dothidea | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Molecular characterization of a novel ourmia‐like virus infecting the plant-pathogenic fungus Botryosphaeria dothidea Shunpei Xie, Haiyan Wu, Fengyue Gong, Xiuyuan Sun, Ruobing Kong, and 6 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-7296888/v1 This work is licensed under a CC BY 4.0 License Status: Published Journal Publication published 07 Jan, 2026 Read the published version in Archives of Virology → Version 1 posted 5 You are reading this latest preprint version Abstract Here, we describe a novel ourmia-like virus, Botryosphaeria dothidea ourmia-like virus 3 (BdOLV3), derived from the phytopathogenic fungus Botryosphaeria dothidea strain ZM211299-2 infecting apple shoots in Shandong province of China. The complete genome sequence of BdOLV3 consists of a positive-sense single-stranded RNA (+ ssRNA) segment with a length of 2,650 nucleotides (nt). The sequence contains a large open reading frame (ORF) encoding a putative RNA-dependent RNA polymerase (RdRp) consisting of 673 amino acids (aa) with a molecular mass of 75.53 kDa. This RdRp protein contains eight typical conserved motifs associated with ourmia-like viruses. BLASTp analysis revealed that the RdRp protein of BdOLV3 had the highest similarity (61.98%, and 51.47% identity) to viruses previously identified as Plasmopara viticola lesion associated ourmia-like virus 45, and Magnaporthe oryzae botourmiavirus 7, respectively. Phylogenetic analysis based on the RdRp aa sequence indicated that BdOLV3 is a new member of the genus Epsilonscleorulivirus in the family Botourmiaviridae . This study is the first to report the complete genomic sequence of the genus Epsilonscleorulivirus in B. dothidea . Full Text Supplementary Files BdOLV3SupplementaryFileS1.docx BdOLV3SupplementaryFileS2.docx Cite Share Download PDF Status: Published Journal Publication published 07 Jan, 2026 Read the published version in Archives of Virology → Version 1 posted Editorial decision: Minor Revision 03 Nov, 2025 Reviewers agreed at journal 11 Sep, 2025 Reviewers invited by journal 10 Sep, 2025 Editor assigned by journal 07 Aug, 2025 First submitted to journal 04 Aug, 2025 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-7296888","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":513023158,"identity":"cb2e1dd6-844f-4444-8d40-a0ab79c713f0","order_by":0,"name":"Shunpei Xie","email":"","orcid":"","institution":"Henan Agricultural University","correspondingAuthor":false,"prefix":"","firstName":"Shunpei","middleName":"","lastName":"Xie","suffix":""},{"id":513023159,"identity":"19ff830b-ceb5-4e17-9f97-8418bd28208a","order_by":1,"name":"Haiyan Wu","email":"","orcid":"","institution":"Henan Agricultural 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