High endogenous phytohormone concentrations are present in cucumber fruit apoplasts

High endogenous phytohormone concentrations are present in cucumber fruit apoplasts | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Help Center Sign In Submit a Preprint Cite Share Download PDF Short Report High endogenous phytohormone concentrations are present in cucumber fruit apoplasts Kiyohide Kojima This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-1259508/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Apoplast (AP) and symplast (SY) solutions were collected by centrifugation from sections of cucumber fruit pulp and placenta. Indole-3-acetic acid (IAA), abscisic acid (ABA), trans-zeatin (tZ), isopentenyladenine (iP), jasmonic acid (JA), methyl jasmonic acid (MeJA), gibberellin 1 (GA 1 ), and gibberellin 4 (GA 4 ) in AP solution, SY solution, and pericarp tissue were simultaneously quantified by liquid chromatography-mass spectrometry (LC-MS). IAA concentrations were 4.0 times higher in AP solution than in SY solution in the placenta and 6.5 times higher in AP solution than in SY solution in the flesh. tZ concentrations were 2 times higher in AP solution than in SY solution in the placenta and 5.5 times higher in AP solution than in SY solution in the flesh. iP concentrations were 2 times higher in AP solution than in SY solution in the placenta and 5.5 times higher in AP solution than in SY solution in the flesh. The concentration of GA 1 was 8 times higher than that of SY solution in the placenta and 3.5 times higher than that of SY solution in the flesh. The concentration of ABA in the placenta was 1.5 times higher than that in SY solution, and that in the flesh was 2 times higher than that in SY solution. The concentrations of the major phytohormones quantified in cucumber fruit were higher in AP solution than in SY solution in both the flesh and placenta. ABA Cytokinin GA IAA Phytohormone Figures Figure 1 Figure 2 Figure 3 Figure 4 Figure 5 Full Text Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. 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Also discoverable on Platform About Our Team In Review Editorial Policies Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-1259508","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Short Report","associatedPublications":[],"authors":[{"id":91774425,"identity":"53220ce9-760b-4025-8735-649848fc097f","order_by":0,"name":"Kiyohide Kojima","email":"data:image/png;base64,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","orcid":"https://orcid.org/0000-0003-0425-1125","institution":"Niigata University","correspondingAuthor":true,"submittingAuthor":false,"prefix":"","firstName":"Kiyohide","middleName":"","lastName":"Kojima","suffix":""}],"badges":[],"createdAt":"2022-01-14 06:12:40","currentVersionCode":1,"declarations":"","doi":"10.21203/rs.3.rs-1259508/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-1259508/v1","draftVersion":[],"editorialEvents":[],"editorialNote":"","failedWorkflow":false,"files":[{"id":19431181,"identity":"f4360c9b-e516-41a4-8c41-e3695106dadd","added_by":"auto","created_at":"2022-03-21 13:33:06","extension":"png","order_by":1,"title":"Figure 1","display":"","copyAsset":false,"role":"figure","size":31954,"visible":true,"origin":"","legend":"\u003cp\u003eAP solution and SY solution of placenta and flesh of cucumber fruits and fresh weight of pericarp. The AP solution and SY solution of 24 individuals were mixed and measured.\u003c/p\u003e","description":"","filename":"1.png","url":"https://assets-eu.researchsquare.com/files/rs-1259508/v1/ff6356e1e5aee3bf5e35c300.png"},{"id":19431183,"identity":"288e2d95-2645-427c-929f-4fec367e4eca","added_by":"auto","created_at":"2022-03-21 13:33:06","extension":"png","order_by":2,"title":"Figure 2","display":"","copyAsset":false,"role":"figure","size":49771,"visible":true,"origin":"","legend":"\u003cp\u003eEndogenous IAA concentrations in AP and SY solutions from the placenta and flesh and pericarp of cucumber fruits. The solutions of 24 individuals were mixed into one and analyzed. An internal standard was added, the mixture was extracted with diethyl ether, fractionated, purified by HPLC, and quantified by LC-MS.\u003c/p\u003e","description":"","filename":"2.png","url":"https://assets-eu.researchsquare.com/files/rs-1259508/v1/32773adb507b1947e1f83a72.png"},{"id":19431185,"identity":"4e8f889b-159d-4255-bfbb-d0f5c60c0e84","added_by":"auto","created_at":"2022-03-21 13:33:06","extension":"png","order_by":3,"title":"Figure 3","display":"","copyAsset":false,"role":"figure","size":45276,"visible":true,"origin":"","legend":"\u003cp\u003eEndogenous Z (A) and iP (B) concentrations in AP and SY solutions from the placenta and flesh and pericarp of cucumber fruits. The solutions of 24 individuals were mixed and analyzed. An internal standard was added, extracted with butanol, fractionated, purified by HPLC, and\u0026nbsp;quantified by LC-MS.\u003c/p\u003e","description":"","filename":"3.png","url":"https://assets-eu.researchsquare.com/files/rs-1259508/v1/2aa6d423019d07a94714eb6e.png"},{"id":19431184,"identity":"ee2dd7a9-ea72-49b9-bbb7-7acf14b48489","added_by":"auto","created_at":"2022-03-21 13:33:06","extension":"png","order_by":4,"title":"Figure 4","display":"","copyAsset":false,"role":"figure","size":48202,"visible":true,"origin":"","legend":"\u003cp\u003eEndogenous GA1 (A) and GA4 (B) concentrations in AP and SY solutions from the placenta and flesh and pericarp of cucumber fruits. The solutions of 24 individuals were mixed and analyzed. An internal standard was added, the mixture was extracted with ethyl acetate, fractionated, purified by HPLC, and quantified by LC-MS.\u003c/p\u003e","description":"","filename":"4.png","url":"https://assets-eu.researchsquare.com/files/rs-1259508/v1/160e6b77d2b1cf4aafbf0240.png"},{"id":19431182,"identity":"291a2bd9-49f2-4800-82f3-173d13813594","added_by":"auto","created_at":"2022-03-21 13:33:06","extension":"png","order_by":5,"title":"Figure 5","display":"","copyAsset":false,"role":"figure","size":36260,"visible":true,"origin":"","legend":"\u003cp\u003eEndogenous ABA concentrations in AP and SY solutions from the placenta and flesh and pericarp of cucumber fruits. The solutions of 24 individuals were mixed and analyzed. An internal standard was added, the mixture was extracted with diethyl ether, fractionated, purified by HPLC, and quantified by LC-MS.\u003c/p\u003e","description":"","filename":"5.png","url":"https://assets-eu.researchsquare.com/files/rs-1259508/v1/80e71c0f55f795df08461aa5.png"},{"id":19431186,"identity":"ff7e232e-7a10-454e-8bc8-3342e9beb3a7","added_by":"auto","created_at":"2022-03-21 13:33:11","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":419634,"visible":true,"origin":"","legend":"","description":"","filename":"2022.1.18APCucumberKojima.pdf","url":"https://assets-eu.researchsquare.com/files/rs-1259508/v1_covered.pdf"}],"financialInterests":"","formattedTitle":"High endogenous phytohormone concentrations are present in cucumber fruit apoplasts","fulltext":[{"header":"Full Text","content":"This preprint is available for \u003ca href='/article/rs-1259508/latest.pdf' target='_blank'\u003edownload as a PDF\u003c/a\u003e."}],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":true,"highlight":"","institution":"","isAcceptedByJournal":false,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":false,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true},"keywords":"ABA, Cytokinin, GA, IAA, Phytohormone","lastPublishedDoi":"10.21203/rs.3.rs-1259508/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-1259508/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"Apoplast (AP) and symplast (SY) solutions were collected by centrifugation from sections of cucumber fruit pulp and placenta. Indole-3-acetic acid (IAA), abscisic acid (ABA), trans-zeatin (tZ), isopentenyladenine (iP), jasmonic acid (JA), methyl jasmonic acid (MeJA), gibberellin 1 (GA 1 ), and gibberellin 4 (GA 4 ) in AP solution, SY solution, and pericarp tissue were simultaneously quantified by liquid chromatography-mass spectrometry (LC-MS). IAA concentrations were 4.0 times higher in AP solution than in SY solution in the placenta and 6.5 times higher in AP solution than in SY solution in the flesh. tZ concentrations were 2 times higher in AP solution than in SY solution in the placenta and 5.5 times higher in AP solution than in SY solution in the flesh. iP concentrations were 2 times higher in AP solution than in SY solution in the placenta and 5.5 times higher in AP solution than in SY solution in the flesh. The concentration of GA 1 was 8 times higher than that of SY solution in the placenta and 3.5 times higher than that of SY solution in the flesh. The concentration of ABA in the placenta was 1.5 times higher than that in SY solution, and that in the flesh was 2 times higher than that in SY solution. The concentrations of the major phytohormones quantified in cucumber fruit were higher in AP solution than in SY solution in both the flesh and placenta.","manuscriptTitle":"High endogenous phytohormone concentrations are present in cucumber fruit apoplasts","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2022-03-21 13:33:05","doi":"10.21203/rs.3.rs-1259508/v1","editorialEvents":[{"type":"communityComments","content":0}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"cc902176-ddd9-4fc3-b091-d411a5ba1d4d","owner":[],"postedDate":"March 21st, 2022","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"posted","subjectAreas":[],"tags":[],"updatedAt":"2022-09-01T06:53:21+00:00","versionOfRecord":[],"versionCreatedAt":"2022-03-21 13:33:05","video":"","vorDoi":"","vorDoiUrl":"","workflowStages":[]},"version":"v1","identity":"rs-1259508","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-1259508","identity":"rs-1259508","version":["v1"]},"buildId":"GqpaHPwrfC8PjnIFayRh5","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}