Maintenance and characterization of human myometrial smooth muscle cells in monolayer culture

M L Casey, M.Linette Casey, P. C. MacDonald, Paul C. MacDonald, Murray D. Mitchell, M D Mitchell, J M Snyder, Jeanne M. Snyder
In vitro · 1984 · vol. 20(5) , pp. 396–403 · doi:10.1007/bf02619585 · PMID:6724619 · W2057328367
Lab / animal OA: closed CC0 ⤵ 3 in-corpus citations
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Human myometrial cells dispersed by enzymatic digestion can be maintained in monolayer culture and retain morphological and biochemical characteristics of smooth muscle cells.

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Abstract

Human myometrial cells were dispersed from uterine tissue by limited enzymatic digestion of myometrium that was obtained at the time of hysterectomy. The dispersed myometrial cells that are obtained in this manner can be maintained in monolayer culture in the presence of medium that contains fetal bovine serum. In primary culture, as well as after passage, the characteristics of these cells are morphologically and biochemically similar to those of smooth muscle cells and myometrial tissue.

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MeSH descriptors

Muscle, Smooth Myometrium Cells, Cultured Culture Media Culture Techniques Culture Techniques DNA DNA Female Humans Kinetics Microscopy, Electron Muscle, Smooth Myometrium Myometrium Proteins Proteins

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References (24)

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organisms 2
human human

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