Time-frequency analysis of 5xFAD mice circadian actigrams as early behavioral diagnostics

Time-frequency analysis of 5xFAD mice circadian actigrams as early behavioral diagnostics | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Time-frequency analysis of 5xFAD mice circadian actigrams as early behavioral diagnostics Alexander Andreev, Evgeniya Akhremenko, Danila Apushkin, Ilya Kovalenko, and 3 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-7538307/v1 This work is licensed under a CC BY 4.0 License Status: Under Revision Version 1 posted 10 You are reading this latest preprint version Abstract Wavelet analysis is a powerful tool for studying behaviors across scales and timing. We used it to analyze spontaneous motor activity in 3-month-old 5xFAD mice and their wild-type littermates, focusing on sex and genotype differences. Mice were placed in home cages 24 hours before recording, and their movements generated oscillations in the environment, which were recorded and analyzed using wavelet transforms. Initially, there were no differences in activity between transgenic and non-transgenic mice for either sex. However, after eight hours, male transgenic mice showed significant differences, lasting from 64 to 256 minutes, while females exhibited disparities after twelve hours, with events lasting 32 to 128 minutes. Transgenic mice had lower interaction levels with their environment during the second half of the experiment. These findings suggest that genotype influences spontaneous motor activity, aligning with human data and supporting the use of non-invasive methods to assess pathology severity and therapeutic efficacy in preclinical studies. Detecting frequency and timing discrepancies between transgenic and non-transgenic animals provides valuable diagnostic insights. Experimental environment homecage spontaneous locomotor activity 5xFAD wavelet Full Text Additional Declarations No competing interests reported. Supplementary Files SupplementaryFigures.docx Cite Share Download PDF Status: Under Revision Version 1 posted Editorial decision: Revision requested 13 Mar, 2026 Reviews received at journal 12 Mar, 2026 Reviewers agreed at journal 09 Feb, 2026 Reviewers agreed at journal 09 Dec, 2025 Reviews received at journal 07 Oct, 2025 Reviewers agreed at journal 15 Sep, 2025 Reviewers invited by journal 06 Sep, 2025 Editor assigned by journal 05 Sep, 2025 Submission checks completed at journal 05 Sep, 2025 First submitted to journal 04 Sep, 2025 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-7538307","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":512280307,"identity":"90aafc99-5de8-44cd-8cc8-6aa70ccfb11c","order_by":0,"name":"Alexander Andreev","email":"","orcid":"","institution":"Perm State University","correspondingAuthor":false,"prefix":"","firstName":"Alexander","middleName":"","lastName":"Andreev","suffix":""},{"id":512280309,"identity":"97bb4c19-e469-4715-99e6-4faf6890494b","order_by":1,"name":"Evgeniya Akhremenko","email":"","orcid":"","institution":"Perm State University","correspondingAuthor":false,"prefix":"","firstName":"Evgeniya","middleName":"","lastName":"Akhremenko","suffix":""},{"id":512280311,"identity":"7acfa63e-bd5c-4e2f-9e17-b1cefa37e4ab","order_by":2,"name":"Danila Apushkin","email":"","orcid":"","institution":"Perm State University","correspondingAuthor":false,"prefix":"","firstName":"Danila","middleName":"","lastName":"Apushkin","suffix":""},{"id":512280313,"identity":"5d6e869d-fba5-4628-af8b-03b638170e3d","order_by":3,"name":"Ilya Kovalenko","email":"","orcid":"","institution":"Perm State University","correspondingAuthor":false,"prefix":"","firstName":"Ilya","middleName":"","lastName":"Kovalenko","suffix":""},{"id":512280315,"identity":"5c2a341e-fa0c-4855-8a79-a407c31f7716","order_by":4,"name":"Kirill Chaprov","email":"","orcid":"","institution":"Federal Research Center of Problems of Chemical Physics and Medicinal Chemistry of the Russian Academy of Sciences","correspondingAuthor":false,"prefix":"","firstName":"Kirill","middleName":"","lastName":"Chaprov","suffix":""},{"id":512280316,"identity":"22dacb10-df7b-41c3-8ab4-4b6331843d77","order_by":5,"name":"Aleksey Ustyugov","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAAAy0lEQVRIiWNgGAWjYDACdjBpw0OCFmYwmUa6lsMk6OBvZj4m8bHtvIw5++EHDD9qGPINDhDQInGYLdlwZtttHsueNAPGnmMMlhsIaWE4zGP4mBeoxeBADgMDbwODAUFb5A/zfzj8t+0cj8H5NwyMf4nRYnCYh/ExY9sBHoMbOQzMRNlieJjN2LDnXDKP5YxnBodljkkYSBLSIne8+ZnEjzI7e3P+5IcP39TYGPAR0gIGjGxAFwJpoGIJYtSDwB+IllEwCkbBKBgFWAEAjmg7nfNpbC8AAAAASUVORK5CYII=","orcid":"","institution":"Federal Research Center of Problems of Chemical Physics and Medicinal Chemistry of the Russian Academy of Sciences","correspondingAuthor":true,"prefix":"","firstName":"Aleksey","middleName":"","lastName":"Ustyugov","suffix":""},{"id":512280317,"identity":"06becf3e-3aee-4a7c-b526-f735c8c595df","order_by":6,"name":"Vladimir Nebogatikov","email":"","orcid":"","institution":"Federal Research Center of Problems of Chemical Physics and Medicinal Chemistry of the Russian Academy of Sciences","correspondingAuthor":false,"prefix":"","firstName":"Vladimir","middleName":"","lastName":"Nebogatikov","suffix":""}],"badges":[],"createdAt":"2025-09-04 17:23:22","currentVersionCode":1,"declarations":"","doi":"10.21203/rs.3.rs-7538307/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-7538307/v1","draftVersion":[],"editorialEvents":[],"editorialNote":"","failedWorkflow":false,"files":[{"id":91168055,"identity":"9a42cb4b-5580-4950-b7e1-49c3b76704f8","added_by":"auto","created_at":"2025-09-12 11:06:15","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":621762,"visible":true,"origin":"","legend":"","description":"","filename":"ustyugov2025.pdf","url":"https://assets-eu.researchsquare.com/files/rs-7538307/v1_covered_4bf639dd-e736-4827-8bdc-8297171785ec.pdf"},{"id":91166750,"identity":"e46123eb-ae60-4d74-aa83-26c3fa4e1347","added_by":"auto","created_at":"2025-09-12 10:42:14","extension":"docx","order_by":0,"title":"","display":"","copyAsset":false,"role":"supplement","size":1272912,"visible":true,"origin":"","legend":"","description":"","filename":"SupplementaryFigures.docx","url":"https://assets-eu.researchsquare.com/files/rs-7538307/v1/370471828ee4a8fd1f2af123.docx"}],"financialInterests":"No competing interests reported.","formattedTitle":"Time-frequency analysis of 5xFAD mice circadian actigrams as early behavioral diagnostics","fulltext":[],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":false,"highlight":"","institution":"","isAcceptedByJournal":false,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":true,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"brain-informatics","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":false,"externalIdentity":"brai","sideBox":"Learn more about [Brain Informatics](https://braininformatics.springeropen.com/about)","snPcode":"40708","submissionUrl":"https://submission.nature.com/new-submission/40708/3","title":"Brain Informatics","twitterHandle":"@SpringerOpen","acdcEnabled":true,"dfaEnabled":true,"editorialSystem":"em","reportingPortfolio":"BMC/SO AJ","inReviewEnabled":true,"inReviewRevisionsEnabled":true},"keywords":"Experimental environment, homecage, spontaneous locomotor activity, 5xFAD, wavelet","lastPublishedDoi":"10.21203/rs.3.rs-7538307/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-7538307/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003eWavelet analysis is a powerful tool for studying behaviors across scales and timing. We used it to analyze spontaneous motor activity in 3-month-old 5xFAD mice and their wild-type littermates, focusing on sex and genotype differences. Mice were placed in home cages 24 hours before recording, and their movements generated oscillations in the environment, which were recorded and analyzed using wavelet transforms. Initially, there were no differences in activity between transgenic and non-transgenic mice for either sex. However, after eight hours, male transgenic mice showed significant differences, lasting from 64 to 256 minutes, while females exhibited disparities after twelve hours, with events lasting 32 to 128 minutes. Transgenic mice had lower interaction levels with their environment during the second half of the experiment. These findings suggest that genotype influences spontaneous motor activity, aligning with human data and supporting the use of non-invasive methods to assess pathology severity and therapeutic efficacy in preclinical studies. Detecting frequency and timing discrepancies between transgenic and non-transgenic animals provides valuable diagnostic insights.\u003c/p\u003e","manuscriptTitle":"Time-frequency analysis of 5xFAD mice circadian actigrams as early behavioral diagnostics","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2025-09-12 10:42:09","doi":"10.21203/rs.3.rs-7538307/v1","editorialEvents":[{"type":"communityComments","content":0},{"type":"decision","content":"Revision requested","date":"2026-03-13T10:03:19+00:00","index":"","fulltext":""},{"type":"editorInvitedReview","content":"","date":"2026-03-13T03:40:17+00:00","index":"hide","fulltext":""},{"type":"reviewerAgreed","content":"82589302451284003944825564186806672714","date":"2026-02-09T08:53:21+00:00","index":"hide","fulltext":""},{"type":"reviewerAgreed","content":"221601429504295446203336841808819098163","date":"2025-12-09T15:23:41+00:00","index":"hide","fulltext":""},{"type":"editorInvitedReview","content":"","date":"2025-10-07T07:26:56+00:00","index":"hide","fulltext":""},{"type":"reviewerAgreed","content":"65737448052692509625754751917875988146","date":"2025-09-15T14:31:28+00:00","index":"hide","fulltext":""},{"type":"reviewersInvited","content":"","date":"2025-09-06T13:42:20+00:00","index":"","fulltext":""},{"type":"editorAssigned","content":"","date":"2025-09-05T14:30:28+00:00","index":"","fulltext":""},{"type":"checksComplete","content":"","date":"2025-09-05T14:29:11+00:00","index":"","fulltext":""},{"type":"submitted","content":"Brain Informatics","date":"2025-09-04T17:18:32+00:00","index":"","fulltext":""}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"brain-informatics","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":false,"externalIdentity":"brai","sideBox":"Learn more about [Brain Informatics](https://braininformatics.springeropen.com/about)","snPcode":"40708","submissionUrl":"https://submission.nature.com/new-submission/40708/3","title":"Brain Informatics","twitterHandle":"@SpringerOpen","acdcEnabled":true,"dfaEnabled":true,"editorialSystem":"em","reportingPortfolio":"BMC/SO AJ","inReviewEnabled":true,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"2fc3b2ab-7076-40f9-89cf-4613962d8333","owner":[],"postedDate":"September 12th, 2025","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"in-revision","subjectAreas":[],"tags":[],"updatedAt":"2026-03-13T10:11:14+00:00","versionOfRecord":[],"versionCreatedAt":"2025-09-12 10:42:09","video":"","vorDoi":"","vorDoiUrl":"","workflowStages":[]},"version":"v1","identity":"rs-7538307","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-7538307","identity":"rs-7538307","version":["v1"]},"buildId":"8U1c8b4HqxoKbykW_rLl7","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}