Nuclear-based labeling of cellular immunotherapies: a simple protocol for preclinical use

Nuclear-based labeling of cellular immunotherapies: a simple protocol for preclinical use | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Nuclear-based labeling of cellular immunotherapies: a simple protocol for preclinical use Alessia Volpe, Serge K. Lyashchenko, Vladimir Ponomarev This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-3890778/v1 This work is licensed under a CC BY 4.0 License Status: Published Journal Publication published 03 Jul, 2024 Read the published version in Molecular Imaging and Biology → Version 1 posted 5 You are reading this latest preprint version Abstract Labeling and tracking existing and emerging cell-based immunotherapies using nuclear imaging is widely used to guide the preclinical phases of development and testing of existing and new emerging off-the-shelf cell-based immunotherapies. In fact, advancing our knowledge about their mechanism of action and limitations could provide preclinical support and justification for moving towards clinical experimentation of newly generated products and expedite their approval by the Food and Drug Administration (FDA). Here we provide the reader with a ready to use protocol describing the labeling methodologies and practical procedures to render different candidate cell therapies in vivo traceable by nuclear-based imaging. The protocol includes sufficient practical details to aid researchers at all career stages and from different fields in familiarizing with the described concepts and incorporating them into their work. nuclear imaging preclinical imaging direct labeling reporter gene labeling cell therapy Full Text Cite Share Download PDF Status: Published Journal Publication published 03 Jul, 2024 Read the published version in Molecular Imaging and Biology → Version 1 posted Editorial decision: Minor revisions 11 Mar, 2024 Reviewers agreed at journal 25 Jan, 2024 Reviewers invited by journal 25 Jan, 2024 Editor assigned by journal 23 Jan, 2024 First submitted to journal 22 Jan, 2024 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-3890778","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":269539082,"identity":"62d5ffb5-9c37-45cf-9bee-e6d413430896","order_by":0,"name":"Alessia Volpe","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAAAx0lEQVRIiWNgGAWjYFAC5gYgISHHwMBGtBZGkBYLY5K1VCQ2EK3F4Hhj24MPNRLpa/uPJX74wWCTL+9ASMuZg+2GM45J5G67kXZYsochzXLjAQJaJGcktknzsIG0sLcxMzAcNjBsIEbLn38S6WbnjxOphV8CqIWxTSLB7EDaMbAWeQI6GPh5gH7p7ZMwBPolWbLHIM3AgJAWNvbmYw9+fKuTNzt/zPDDjwobA3lCDmNAjUKgFQYHSNMCBMTYMgpGwSgYBSMLAACU2j3hE8gW8wAAAABJRU5ErkJggg==","orcid":"https://orcid.org/0000-0003-3087-9178","institution":"Memorial Sloan Kettering Cancer Center","correspondingAuthor":true,"prefix":"","firstName":"Alessia","middleName":"","lastName":"Volpe","suffix":""},{"id":269539083,"identity":"bbeda006-873c-4a03-9699-ea5499164d75","order_by":1,"name":"Serge K. 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