Cytotoxicity of Peruvian propolis and Psidium guajava on human gingival fibroblasts, PBMCs and HeLa cells

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However, this must be accompanied by knowledge of their effect on cell lines to ensure their use is safe. The objective of this study was to evaluate the cytotoxic effect of two ethanolic extracts based on Peruvian natural products, on three human cell lines. Cervical cancer cell lines (HeLa), human gingival fibroblasts (HGF-1 - ATCC CRL-2014) (HGF-1) and peripheral blood mononuclear cells (PBMCs) were cultured and subsequently treated with preparations of ethanolic extracts of propolis (EEP) and Psidium guajava (EEG) from a concentration of 50 mg/mL to 0.024 mg/mL, by the 3-(4,5- dimethylthiazol-2-yl)-2,5-diphenyltetrazole bromide reduction assay. At a concentration of 0.24 mg/mL EEG, viability of 99.7±1.24%, 99.8±2.2% and 99.7±2.7% was observed in HeLa, HGF-1 and PBMCs, respectively; >90% cell viability values were observed with EPP at 0.024 mg/mL, with HGF-1 showing the highest viability (96.9±1.15%). A dose-dependent effect was observed for both extracts with a decrease in cell viability as concentrations increased (up to 50 mg/mL). EEP and EEG extracts at low concentrations do not show cytotoxicity in human cell lines, these findings are an advance in the preclinical evaluation on their safety and open a continuity to further studies for their potential applications in dentistry and medicine." } { "@context": "http://schema.org", "@type": "BreadcrumbList", "itemListElement": [ { "@type": "ListItem", "position": "1", "item": { "@id": "https://f1000research.com/", "name": "Home" } }, { "@type": "ListItem", "position": "2", "item": { "@id": "https://f1000research.com/browse/articles", "name": "Browse" } }, { "@type": "ListItem", "position": "3", "item": { "@id": "https://f1000research.com/articles/11-430/v2", "name": "Cytotoxicity of Peruvian propolis and Psidium guajava on human gingival..." } } ] } Home Browse Cytotoxicity of Peruvian propolis and Psidium guajava on human gingival... ALL Metrics - Views Downloads Get PDF Get XML Cite How to cite this article Millones-Gómez PA, De la Garza-Ramos MA, Urrutia-Baca VH et al. Cytotoxicity of Peruvian propolis and Psidium guajava on human gingival fibroblasts, PBMCs and HeLa cells [version 2; peer review: 2 approved] . F1000Research 2022, 11 :430 ( https://doi.org/10.12688/f1000research.110352.2 ) NOTE: If applicable, it is important to ensure the information in square brackets after the title is included in all citations of this article. Close Copy Citation Details Export Export Citation Sciwheel EndNote Ref. Manager Bibtex ProCite Sente EXPORT Select a format first Track Share ▬ ✚ Research Article Revised Cytotoxicity of Peruvian propolis and Psidium guajava on human gingival fibroblasts, PBMCs and HeLa cells [version 2; peer review: 2 approved] Pablo Alejandro Millones-Gómez https://orcid.org/0000-0002-7105-0940 1 , Myriam Angélica De la Garza-Ramos https://orcid.org/0000-0003-0792-3695 2 , Victor Hugo Urrutia-Baca 3 , Humberto Carlos Hernandez-Martinez 3 , David Alejandro Hernández Marín 4 , Carlos Alberto Minchón Medina 5 Pablo Alejandro Millones-Gómez https://orcid.org/0000-0002-7105-0940 1 , Myriam Angélica De la Garza-Ramos https://orcid.org/0000-0003-0792-3695 2 , [...] Victor Hugo Urrutia-Baca 3 , Humberto Carlos Hernandez-Martinez 3 , David Alejandro Hernández Marín 4 , Carlos Alberto Minchón Medina 5 PUBLISHED 16 Aug 2022 Author details Author details 1 Escuela de Medicina Humana, Universidad Señor de Sipán, Chiclayo, 14000, Peru 2 Facultad de Odontologia, CIDICS/UOIE, Universidad Autónoma de Nuevo León, Monterrey, 64000, Mexico 3 Facultad de Ciencias Biológicas, Laboratorio de Inmunología y Virología/ CIDICS/UOIE, Universidad Autónoma de Nuevo León, Monterrey, 64000, Mexico 4 Departamento de Microbiología, Centro de Ciencias Básicas, Universidad Autónoma de Aguascalientes, Aguascalientes, 20100, Mexico 5 Department of Statistics, Faculty of Physical Sciences and Mathematics, Universidad Nacional de Trujillo, Trujillo, 13001, Peru Pablo Alejandro Millones-Gómez Roles: Conceptualization, Data Curation, Formal Analysis, Funding Acquisition, Investigation, Methodology, Project Administration, Resources, Software, Supervision, Validation, Visualization, Writing – Original Draft Preparation, Writing – Review & Editing Myriam Angélica De la Garza-Ramos Roles: Methodology, Validation Victor Hugo Urrutia-Baca Roles: Formal Analysis, Methodology, Writing – Review & Editing Humberto Carlos Hernandez-Martinez Roles: Formal Analysis, Methodology, Writing – Review & Editing David Alejandro Hernández Marín Roles: Formal Analysis, Methodology, Writing – Review & Editing Carlos Alberto Minchón Medina Roles: Data Curation, Formal Analysis, Writing – Original Draft Preparation, Writing – Review & Editing OPEN PEER REVIEW DETAILS REVIEWER STATUS This article is included in the Cell & Molecular Biology gateway. Abstract It is indisputable that every day it is demonstrated that natural products present diverse therapeutic benefits, which has boosted their incorporation within various products for clinical use. However, this must be accompanied by knowledge of their effect on cell lines to ensure their use is safe. The objective of this study was to evaluate the cytotoxic effect of two ethanolic extracts based on Peruvian natural products, on three human cell lines. Cervical cancer cell lines (HeLa), human gingival fibroblasts (HGF-1 - ATCC CRL-2014) (HGF-1) and peripheral blood mononuclear cells (PBMCs) were cultured and subsequently treated with preparations of ethanolic extracts of propolis (EEP) and Psidium guajava (EEG) from a concentration of 50 mg/mL to 0.024 mg/mL, by the 3-(4,5- dimethylthiazol-2-yl)-2,5-diphenyltetrazole bromide reduction assay. At a concentration of 0.24 mg/mL EEG, viability of 99.7±1.24%, 99.8±2.2% and 99.7±2.7% was observed in HeLa, HGF-1 and PBMCs, respectively; >90% cell viability values were observed with EPP at 0.024 mg/mL, with HGF-1 showing the highest viability (96.9±1.15%). A dose-dependent effect was observed for both extracts with a decrease in cell viability as concentrations increased (up to 50 mg/mL). EEP and EEG extracts at low concentrations do not show cytotoxicity in human cell lines, these findings are an advance in the preclinical evaluation on their safety and open a continuity to further studies for their potential applications in dentistry and medicine. READ ALL READ LESS Keywords Cytotoxicity, Viability, Propolis, Guava Corresponding Author(s) Pablo Alejandro Millones-Gómez ( [email protected] ) Close Corresponding author: Pablo Alejandro Millones-Gómez Competing interests: No competing interests were disclosed. Grant information: The author(s) declared that no grants were involved in supporting this work. Copyright: © 2022 Millones-Gómez PA et al . This is an open access article distributed under the terms of the Creative Commons Attribution License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. How to cite: Millones-Gómez PA, De la Garza-Ramos MA, Urrutia-Baca VH et al. Cytotoxicity of Peruvian propolis and Psidium guajava on human gingival fibroblasts, PBMCs and HeLa cells [version 2; peer review: 2 approved] . F1000Research 2022, 11 :430 ( https://doi.org/10.12688/f1000research.110352.2 ) First published: 19 Apr 2022, 11 :430 ( https://doi.org/10.12688/f1000research.110352.1 ) Latest published: 16 Aug 2022, 11 :430 ( https://doi.org/10.12688/f1000research.110352.2 ) Revised Amendments from Version 1 In version 2, some criteria for the selection of cell lines, as well as the concentrations used in the study, have been detailed. In version 2, some criteria for the selection of cell lines, as well as the concentrations used in the study, have been detailed. See the authors' detailed response to the review by Orlando Torres Garcia READ REVIEWER RESPONSES Introduction Since time immemorial, man has tried to mitigate his ailments and prolong his life. This fact has been observed since there have been historical records, from civilization to civilization, until today. 1 Even so, man in the 21st century has not been able to avoid death by limiting himself to mitigating symptoms of diseases and avoiding the development of others. 1 , 2 In times when man only had at his disposal the resources that the planet gave him, he sought in these the tools to reduce physical pain and avoid death. Among the resources most exploited by different cultures throughout history are mineral, animal and vegetable resources. Until the middle of the 20th century, these were the therapeutic resources par excellence. 2 , 3 Among the kingdoms of nature that contribute, to this day, to reducing symptoms and preventing diseases, the plant kingdom stands out. 4 Plants, thanks to their marvelous and complex metabolism, constitute a true chemical arsenal. Of which only a third is currently known, considering the variety of existing species worldwide, without considering those species already extinct. 3 , 4 Each region of the world developed its own way of healing from medicinal plants, which is unique and characteristic since species endemic to regions were used. 4 Over time, these local characteristic therapies came to form the so-called traditional medicine and, when being preserved by the native peoples, is sometimes called aboriginal or autochthonous medicine, as well as traditional or autochthonous recipes 4 that group together uses, forms of preparation, administration, dosage, among other modern pharmacological parameters. This is because our therapeutic reality today is governed by synthetic chemistry, but what few people know is that these successful molecules that cure are nothing more than improved copies of chemical substances that nature spontaneously created. 4 One of the most studied products is propolis, 5 which is composed of approximately 50% to 55% resins and balsams, 30% to 40% wax, 10% to 15% essential oils, 5% pollen and 5% minerals. 5 In its components we can mention that it has phenolic compounds: Flavonoids, flavones, isoflavones and flavonones in 50%, 5 which inhibit bacteria and fungi. 6 The amount of flavonoids confers the antibacterial power to propolis. This quantity depends on the flora surrounding the bee hives. 5 Its antibacterial action mechanism is given by the inhibition of cell division, DNA disruption, disorganization of the cytoplasmic membrane and inhibition of cell wall synthesis, causing partial bacteriolysis and inhibiting protein synthesis. 5 , 6 Guava ( Psidium guajava ) is a fruit native to Central America and the Caribbean, belonging to the Myrtaceae family, distributed in the tropics and subtropics around the world. Guava fruits stand out among tropical fruits not only because of their good organoleptic characteristics (flavor and aroma) but also nutritionally, they are a source of vitamin A, B1, B3 and C, fiber, minerals such as potassium, calcium, iron and phosphorus. 7 The guava also has a relevant content of lycopene, an important carotenoid with therapeutic properties, so it has been widely studied. 7 , 8 Cytotoxic evaluation, as the main factor of biocompatibility, is determined by the cell cultures to be selected for in vitro toxicity testing. 9 Continuous and real-time monitoring allows label-free assessment of cell proliferation, viability and cytotoxicity, revealing the physiological status of the cells. 8 To evaluate the efficacy of natural products, it is not enough to measure their therapeutic effect, but one must be sure that they do not cause deterioration of constituent cells. The aim of this study was to evaluate the cytotoxic effect of ethanolic extracts of propolis and P. guajava on HELA cell lines, human gingival fibroblasts (HGF-1) and peripheral blood mononuclear (PBMCs) cells. Methods Preparation of ethanolic extracts Propolis and P. guajava samples were collected by researchers in the Oxapampa valley, Pasco, Peru following the methodology described by Millones et al. 5 , 8 and subsequently refrigerated until processed. After they were removed from refrigeration they were left for two hours to allow for them to reach room temperature. Once they had reached room temperature they were macerated Once room temperature was reached, they were macerated with a volume of 100 ml of absolute ethanol for every 10 grams of propolis sample, it was then left at room temperature for 24 hours. Then, the macerate was filtered using a 20 cm diameter glass funnel with sterile cotton; the filtered sample was collected in a glass refractory to finally be taken to an extraction hood so that the ethanol present in the extract evaporates completely and only a pasty mass remains. This step was performed two more times until the samples were observed to be discolored. Finally, they were stored in glass containers covered with aluminum foil to avoid degradation. 10 Cell lines and cell culture The evaluation of cytotoxicity was performed considering oral cavity constitutive cell lines, human gingival fibroblasts (HGF-1); immune constitutive cells, peripheral blood mononuclear cells (PBMCs); and tumour constitutive cells, HeLa cell lines. HeLa cell line preparation The HeLa cell line (ATCC, Manassas, VA, USA) (RRID:CVCL_0058) was cultured in a Petri dish with a 35 mm diameter glass bottom (MatTek Corporation, Ashland, MA, USA, CAT#: P DCF OS 30). The cell line was cultured in Eagle’s Minimum Essential Medium ( Gibco) with 4% PBS (phosphate buffered saline) at 37°C in 5% CO 2 and 95% air. 5 The cells were incubated for 30 min in 1 mL of dye solution in Hank’s Balanced Salt Solution (HBSS, Thermo Fisher Scientific)) (100 nM Mitotracker Red FM) at 37°C, 5% CO 2 . After incubation, the cells were washed three times with HBSS buffer. 11 Human gingival fibroblasts culture Human gingival fibroblasts (HGF-1 - ATCC CRL-2014) (HGF-1) were obtained from the American Type Culture Collection (ATCC) and cultured in Dulbecco’s modified Dulbecco’s medium containing glucose (DMEM; Sigma-Aldrich, St. Louis, MO, USA) supplemented with 4 mM L-glutamine (Sigma-Aldrich), 1% penicillin, streptomycin (Sigma-Aldrich) and 10% (vol/vol) heat-inactivated fetal bovine serum (FBS; Sigma-Aldrich). Cells were incubated at 37°C in a 5% CO 2 atmosphere in an incubator (Cytomat 2C450S; Thermo Fisher Scientific), and were fed every 48 hours and subcultured every 5 days at a 1:3 ratio using trypsin-EDTA (0.05%; Sigma-Aldrich) for 3 minutes at 37°C. 12 PBMCs culture PBMC cells were isolated by Ficoll density gradient centrifugation (TBD, Shanghai, China) and cultured in RPMI-1640 medium with 10% fetal bovine serum and placed in a humidified incubator (Thermo CO 2 incubator, 311, USA) at 37°C, 5% CO 2 and 95% humidity. The medium was changed once every 24 hours. 13 – 16 Cytotoxicity assays To evaluate the cytotoxic effect, cell viability methodology was performed in microplate with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazole bromide (MTT). 10 For this, confluent cell cultures of (80-100%) contained in 25 cm 2 flasks were started, the medium was discarded using a 5mL serological pipette, two washes were performed to the cell layer with PBS solution (0.004% Ethylene diamine tetracetic acid), 1 mL of Trypsin-EDTA (0.05%) was immediately added and incubated at 37°C for 15 min. A 20 μL sample was taken and 20 μL of 0.4% trypan blue (Gibco, Carlsbad, CA, USA) was added to perform a cell count in a Neubauer chamber (Sigma-Aldrich, model: Bright-Line TM Hemacytometer, catalog number: Z359629) to adjust the cell concentration to 5×104 cells per reaction with Dulbecco’s Modified Eagle’s medium (d-MEM) supplemented with 10% fetal bovine serum. Then 50 μL of the cell suspension was seeded, incubated at 37° C, 5% CO 2 in an incubator (Cytomat 2C450S; Thermo Fisher Scientific) until confluence was obtained (after 48 hours), then different concentrations previously evaluated in the study by Millones et al. 5 (50 to 0.024 mg/mL) were then applied and the plates were incubated for 48 hours. Only d-MEM culture medium was used as a negative control and as a positive control. The medium used was then discarded and the cell layer was washed with PBS solution (0.004% EDTA) and 50 μL of d-MEM culture medium was added. MTT assay To each well, 20 μL of MTT (5 mg/mL) was added to each culture and then the cultures were incubated for four hours. After this time, the medium was aspirated. 200 μL of dimethyl sulfoxide was added to dissolve the formazan crystals; the plate was left in agitation for 15 minutes microplate shaker (Lab-Line Instrument Inc. Melrose Park, IL) and shaken at 120 revolutions per minute to ensure complete dissolution. Finally, the plate was read at 570 nm on a Smart Spectophotometer plus reader (1705061, Bio-Rad, Hercules, CA, USA). Statistical analysis The experimental data were analyzed using nonlinear regression with the Gompertz model to evaluate the effect according to the concentration of the doses used, whose equation is given by: 32 y = α exp − β e − k x Where, y is the cell viability, x the administered concentration of each product (mg/mL), and α, β, and k are the parameters of the model. Comparison of the cytotoxic effect of propolis and P. guajava was performed using analysis of covariance, which in addition to the product includes the concentration administered. Graphical presentation was prioritized to highlight some analyses. The analyses were performed with Excel (Microsoft Corporation, US, 2019) (RRID:SCR_016137) and SPSS version 26 (IBM, US, 2019) (RRID:SCR_016479). Results The cytotoxic effect of Peruvian propolis is shown in Figure 1 , with high cell viability at concentrations of 0.24 mg/mL, reaching 1.120±0.012 HBA cells, 0.922±0.011 HGF-1 and 0.624±0.002 PBMCs cells, decreasing rapidly to 0.052±0.002, 0.051±0.001 and 0.055±0.001, respectively as concentrations increase up to 50 mg/mL. The estimated nonlinear Gompertz regression models were: HELA cells : y = 437294.952 exp − 12.972 e 0.249 x , R 2 = 0.927 Gingival fibroblast : y = 186292.087 exp − 12.027 e 0.782 x , R 2 = 0.960 PBMC : y = 206045.920 exp − 12.601 e 0.618 x , R 2 = 0.917 Figure 1. Viability of cell lines by effect of concentrations of ethanolic extracts of propolis. The cytotoxic effect of Peruvian propolis is shown in Figure 1 , with high cell viability at concentrations of 0.24 mg/mL, reaching 1.120±0.012 Henrietta Lacks cells (HELA cells), 0.922±0.011 human gingival fibroblasts (HGF-1) and 0.624±0.002 peripheral blood mononuclear cells (PBMCs cells), decreasing rapidly to 0.052±0.002, 0.051±0.001 and 0.055±0.001, respectively as concentrations increase up to 50 mg/mL. The observed trend shows that cell growth decreases in a non-linear fashion as the dose of Peruvian propolis administered increases. On the other hand, the cytotoxic effect of Peruvian P. guajava is shown in Figure 2 , with high cell viability at concentrations of 0.24 mg/mL, reaching 1.190±0.015 HBA cells, 0.948±0.020 HGF-1 and 0.685±0.019 PBMCs cells. These decreased more slowly to 0.656±0.019, 0.165±0.020 and 0.099±0.002, respectively as concentrations increase up to 50 mg/mL. The estimated nonlinear Gompertz regression models were: HELA cells : y = 437294.952 exp − 12.972 e 0.249 x , R 2 = 0.974 Gingival fibroblast : y = 186292.087 exp − 12.027 e 0.782 x , R 2 = 0.970 PBMC : y = 206045.920 exp − 12.601 e 0.618 x , R 2 = 0.982 Figure 2. Viability of cell lines by the effect of concentrations of ethanolic extracts of P. guajava. The cytotoxic effect of guajava is shown in Figure 2 , with high cell viability at concentrations of 0.24 mg/mL, reaching 1.190±0.015 Henrietta Lacks cells (HELA cells), 0.948±0.020 human gingival fibroblasts (HGF-1) and 0.685±0.019 peripheral blood mononuclear cells (PBMCs cells). These decreased more slowly to 0.656±0.019, 0.165±0.020 and 0.099±0.002, respectively as concentrations increase up to 50 mg/mL. Similarly, the observed trend shows that cell growth decreases in a non-linear fashion as the dose of guajava administered increases. The goodness of fit of the Gompertz curves to the cytotoxic effect on the cell lines of both products is shown by the coefficient of determination (R 2 ), this value is above 90% for all curves. The cytotoxic effect of propolis and P. guajava on the cell lines was compared using analysis of covariance, which are shown in Table 1 . In each of the cell lines, differences in the cytotoxic effect between both products were observed (p<0.01 in each of the lines); likewise, the linear effect of the concentrations used for each of the products was observed (p<0.01 in each of the lines), even though the non-linear effect was verified by means of the Gompertz model. Table 1. Analysis of covariance cytotoxic effect of ethanolic extract concentrations of propolis and P. guajava on cell line viability. Cell line Source of variation Sum of squares Degrees of freedom Mean square F p HELA cells Model 3.847 2 1.923 33.170 0.000 Products 3.200 1 3.200 55.185 0.000 Concentrations 0.647 1 0.647 11.155 0.003 Error 1.218 21 0.058 Total 5.064 23 Gingival fibroblast Model 1,899 2 0.949 14.640 0.000 Products 1.225 1 1.225 18.891 0.000 Concentrations 0.674 1 0.674 10.389 0.004 Error 1.362 21 0.065 Total 3.260 23 PBMC Model 0.745 2 0.373 11.200 0.000 Products 0.428 1 0.428 12.867 0.002 Concentrations 0.317 1 0.317 9.533 0.006 Error 0.699 21 0.033 Total 1.444 23 Figures 3 - 5 show the cytotoxic effect of propolis and P. guajava on the cell lines as a percentage of cell viability, established from the medium controls and Triton X-100, being notorious the differences in the effect between both products, as already shown. Figure 3. Viability of HELA cells by effect of concentrations of ethanolic extracts of propolis and P. guajava. Figure 3 compares the cytotoxic effect of propolis and P. guajava concentrations and Triton X-100 on Henrietta Lacks cells (HELA cells) viability (%), showing the average and the corresponding standard deviations. With propolis, the growth of HELA cells remains in control with doses of 50-1,563 mg/mL, increasing very rapidly with smaller doses. In contrast, with guajava, cell growth is already more than 50% at doses of 50 mg/mL, growing rapidly, and reaching maximum levels at doses of 1,563 mg/mL or lower. Figure 4. Viability of human gingival fibroblasts by effect of ethanolic extracts concentrations of propolis and P. guajava. The growth of human gingival fibroblasts remains in control with propolis doses of 50-0.39 mg/mL, with rapid growth at lower doses; in contrast, with gujava, while at doses of 50 mg/mL cell growth was pc more than 10%, it begins to increase considerably up to doses of 0.781 mg/mL, at which it reaches maximum growths. Figure 5. Viability of peripheral blood mononuclear cells (PBMCs) by effect of concentrations of ethanolic extracts of propolis and P. guajava. The growth of peripheral blood mononuclear cells (PBMCs cells) remains in control with propolis doses of 50-0.39 mg/mL, with rapid growth at lower doses; on the contrary, with guajava, although at a dose of 50 mg/mL cell growth was small, it begins to increase considerably up to doses of 0.195 mg/mL, at which it reaches maximum growth. Discussion The aim of this study was to evaluate the cytotoxic effect of ethanolic extracts of propolis and P. guajava on HELA cell lines, HGF-1 and PBMCs cells. The results showed high cell viability at concentrations of 0.24 mg/mL, decreasing rapidly as concentrations increased up to 50 mg/mL. The cytotoxicity assay revealed that Peruvian propolis and guajava extracts at lower concentrations can work safely on the fibroblast cell line. However, it is important to recognize that since this is an in vitro assay, this value may vary if other types of cell lines are used. The results obtained in the cytotoxicity assay, add to the increasingly abundant information reported by other research groups that have wanted to address this issue but focusing on alcoholic extracts, 17 – 19 and are therefore important. The content of phenols and flavonoids obtained in the aqueous extract of propolis is lower than that reported by other research groups 20 however, it should be noted that the extraction methods were different and as mentioned, these are determinant for the preparation of the extract. To determine which methodology is more efficient, the same propolis sample should be used, 21 – 24 due to the high variability in the composition that this product may have with respect to its region of origin. 24 As for P. guajava , it showed similar toxicity in the three cell lines. Some studies report a higher amount of active metabolites in the peel with respect to guava pulp, and also report a better antioxidant capacity in vitro. 24 , 25 With the cytotoxic effect shown in HeLa cells, the world list of plants with potential for cases of cervical neoplasia published is increased, the results obtained in the study contribute to corroborate the properties traditionally attributed to these plants and highlight species of the Peruvian medicinal flora as a source of substances for the treatment of cancer. 25 Despite the high cytotoxicity shown by most of the propolis samples against the cell lines studied, the samples also showed toxicity to HGF-1 culture. Ling et al . 26 have also investigated the cytotoxicity of Brazilian red propolis extracts for two tumor cell lines (Hep-2 and HeLa) and for normal human embryonic epithelial kidney (Hek-293), also reporting a higher IC 50 value for Hek-293 compared to the tumor cell lines. Although both propolis and guava seem to exert a potential on PBMCs cells, only a few studies have been performed in this field of research. 27 – 30 Despite this, our preliminary data suggest that both products could have a significant biological effect on the three cell lines tested, which opens up prospects for further research in this field. Conclusions EEP and EEG at low concentrations do not show cytotoxicity in human cell lines and their effect is dose dependent. Our findings are an advance in the preclinical evaluation of natural extracts from Peru on their safety and open a continuity to further studies for their potential applications in dentistry and medicine. Despite our positive data, further study is required to evaluate the usefulness of these extracts. 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PubMed Abstract | Publisher Full Text Comments on this article Comments (0) Version 2 VERSION 2 PUBLISHED 19 Apr 2022 ADD YOUR COMMENT Comment Author details Author details 1 Escuela de Medicina Humana, Universidad Señor de Sipán, Chiclayo, 14000, Peru 2 Facultad de Odontologia, CIDICS/UOIE, Universidad Autónoma de Nuevo León, Monterrey, 64000, Mexico 3 Facultad de Ciencias Biológicas, Laboratorio de Inmunología y Virología/ CIDICS/UOIE, Universidad Autónoma de Nuevo León, Monterrey, 64000, Mexico 4 Departamento de Microbiología, Centro de Ciencias Básicas, Universidad Autónoma de Aguascalientes, Aguascalientes, 20100, Mexico 5 Department of Statistics, Faculty of Physical Sciences and Mathematics, Universidad Nacional de Trujillo, Trujillo, 13001, Peru Pablo Alejandro Millones-Gómez Roles: Conceptualization, Data Curation, Formal Analysis, Funding Acquisition, Investigation, Methodology, Project Administration, Resources, Software, Supervision, Validation, Visualization, Writing – Original Draft Preparation, Writing – Review & Editing Myriam Angélica De la Garza-Ramos Roles: Methodology, Validation Victor Hugo Urrutia-Baca Roles: Formal Analysis, Methodology, Writing – Review & Editing Humberto Carlos Hernandez-Martinez Roles: Formal Analysis, Methodology, Writing – Review & Editing David Alejandro Hernández Marín Roles: Formal Analysis, Methodology, Writing – Review & Editing Carlos Alberto Minchón Medina Roles: Data Curation, Formal Analysis, Writing – Original Draft Preparation, Writing – Review & Editing Competing interests No competing interests were disclosed. Grant information The author(s) declared that no grants were involved in supporting this work. Article Versions (2) version 2 Revised Published: 16 Aug 2022, 11:430 https://doi.org/10.12688/f1000research.110352.2 version 1 Published: 19 Apr 2022, 11:430 https://doi.org/10.12688/f1000research.110352.1 Copyright © 2022 Millones-Gómez PA et al . This is an open access article distributed under the terms of the Creative Commons Attribution License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Download Export To Sciwheel Bibtex EndNote ProCite Ref. Manager (RIS) Sente metrics Views Downloads F1000Research - - PubMed Central info_outline Data from PMC are received and updated monthly. - - Citations open_in_new 0 open_in_new 0 open_in_new SEE MORE DETAILS CITE how to cite this article Millones-Gómez PA, De la Garza-Ramos MA, Urrutia-Baca VH et al. Cytotoxicity of Peruvian propolis and Psidium guajava on human gingival fibroblasts, PBMCs and HeLa cells [version 2; peer review: 2 approved] . F1000Research 2022, 11 :430 ( https://doi.org/10.12688/f1000research.110352.2 ) NOTE: If applicable, it is important to ensure the information in square brackets after the title is included in all citations of this article. COPY CITATION DETAILS track receive updates on this article Track an article to receive email alerts on any updates to this article. TRACK THIS ARTICLE Share Open Peer Review Current Reviewer Status: ? Key to Reviewer Statuses VIEW HIDE Approved The paper is scientifically sound in its current form and only minor, if any, improvements are suggested Approved with reservations A number of small changes, sometimes more significant revisions are required to address specific details and improve the papers academic merit. Not approved Fundamental flaws in the paper seriously undermine the findings and conclusions Version 2 VERSION 2 PUBLISHED 16 Aug 2022 Revised Views 0 Cite How to cite this report: Torres Garcia O. Reviewer Report For: Cytotoxicity of Peruvian propolis and Psidium guajava on human gingival fibroblasts, PBMCs and HeLa cells [version 2; peer review: 2 approved] . F1000Research 2022, 11 :430 ( https://doi.org/10.5256/f1000research.137410.r147645 ) The direct URL for this report is: https://f1000research.com/articles/11-430/v2#referee-response-147645 NOTE: it is important to ensure the information in square brackets after the title is included in this citation. Close Copy Citation Details Reviewer Report 17 Aug 2022 Orlando Torres Garcia , Faculty of Veterinary Medicine and Zootechnics, Universidad Antonio Nariño, Bogotá, Colombia; Faculty of Sciences, Javeriana University, Bogotá, Colombia Approved VIEWS 0 https://doi.org/10.5256/f1000research.137410.r147645 The document is interesting and presents two compounds of natural origin ... Continue reading READ ALL The document is interesting and presents two compounds of natural origin (Propolis and Psidium guajava) with potential for medical and dental application. Competing Interests: No competing interests were disclosed. Reviewer Expertise: Immunology and Immunogenetics, microbiology. I confirm that I have read this submission and believe that I have an appropriate level of expertise to confirm that it is of an acceptable scientific standard. Close READ LESS CITE CITE HOW TO CITE THIS REPORT Torres Garcia O. Reviewer Report For: Cytotoxicity of Peruvian propolis and Psidium guajava on human gingival fibroblasts, PBMCs and HeLa cells [version 2; peer review: 2 approved] . F1000Research 2022, 11 :430 ( https://doi.org/10.5256/f1000research.137410.r147645 ) The direct URL for this report is: https://f1000research.com/articles/11-430/v2#referee-response-147645 NOTE: it is important to ensure the information in square brackets after the title is included in all citations of this article. COPY CITATION DETAILS Report a concern Respond or Comment COMMENT ON THIS REPORT Views 0 Cite How to cite this report: Rosales Cifuentes TV. Reviewer Report For: Cytotoxicity of Peruvian propolis and Psidium guajava on human gingival fibroblasts, PBMCs and HeLa cells [version 2; peer review: 2 approved] . F1000Research 2022, 11 :430 ( https://doi.org/10.5256/f1000research.137410.r147646 ) The direct URL for this report is: https://f1000research.com/articles/11-430/v2#referee-response-147646 NOTE: it is important to ensure the information in square brackets after the title is included in this citation. Close Copy Citation Details Reviewer Report 17 Aug 2022 Tania Valentina Rosales Cifuentes , Universidad Nacional Mayor de San Marcos, Lima, Peru Approved VIEWS 0 https://doi.org/10.5256/f1000research.137410.r147646 I confirm that I have read this submission and believe that I have an ... Continue reading READ ALL Competing Interests: No competing interests were disclosed. Reviewer Expertise: Natural products and microbiology I confirm that I have read this submission and believe that I have an appropriate level of expertise to confirm that it is of an acceptable scientific standard. Close READ LESS CITE CITE HOW TO CITE THIS REPORT Rosales Cifuentes TV. Reviewer Report For: Cytotoxicity of Peruvian propolis and Psidium guajava on human gingival fibroblasts, PBMCs and HeLa cells [version 2; peer review: 2 approved] . F1000Research 2022, 11 :430 ( https://doi.org/10.5256/f1000research.137410.r147646 ) The direct URL for this report is: https://f1000research.com/articles/11-430/v2#referee-response-147646 NOTE: it is important to ensure the information in square brackets after the title is included in all citations of this article. COPY CITATION DETAILS Report a concern Respond or Comment COMMENT ON THIS REPORT Version 1 VERSION 1 PUBLISHED 19 Apr 2022 Views 0 Cite How to cite this report: Torres Garcia O. Reviewer Report For: Cytotoxicity of Peruvian propolis and Psidium guajava on human gingival fibroblasts, PBMCs and HeLa cells [version 2; peer review: 2 approved] . F1000Research 2022, 11 :430 ( https://doi.org/10.5256/f1000research.121947.r144832 ) The direct URL for this report is: https://f1000research.com/articles/11-430/v1#referee-response-144832 NOTE: it is important to ensure the information in square brackets after the title is included in this citation. Close Copy Citation Details Reviewer Report 09 Aug 2022 Orlando Torres Garcia , Faculty of Veterinary Medicine and Zootechnics, Universidad Antonio Nariño, Bogotá, Colombia; Faculty of Sciences, Javeriana University, Bogotá, Colombia Approved with Reservations VIEWS 0 https://doi.org/10.5256/f1000research.121947.r144832 In this article, the authors evaluated the cytotoxic effect of ethanolic extracts of propolis and P. guajava on HELA cell lines, human gingival fibroblasts (HGF-1), and peripheral blood mononuclear cells (PBMC), finding cell viability more significant than 90% at concentrations ... Continue reading READ ALL In this article, the authors evaluated the cytotoxic effect of ethanolic extracts of propolis and P. guajava on HELA cell lines, human gingival fibroblasts (HGF-1), and peripheral blood mononuclear cells (PBMC), finding cell viability more significant than 90% at concentrations equal to or less than 0.24 mg/mL and 0.024 mg/mL for EEG and EPP, respectively. Within this perspective, the authors infer potential applications in medicine and dentistry. Main comments: In the study methodology, the authors use the HELA cell lines, human gingival fibroblasts (HGF-1), and peripheral blood mononuclear cells (PBMC); however, the reason for their selection they not mentioned in the text. I suggest the authors include some selection criteria for these cell lines used in work. To determine the cytotoxicity, the authors use the ethanolic extracts of propolis and P. guajava at the concentration range of 0.024 to 50 mg/mL. What were the selection criteria for this range of concentrations? Although the document achieves the objective of determining the highest concentrations of the two ethanolic extracts at which cell viabilities more significant than 90% are observed, it would also be interesting to calculate the IC50 of each extract on each cell line. Minor comment: In the last paragraph of the discussion, the authors state that their preliminary data suggest that both products could have a modulating action on the immune response. I suggest rethinking this inference because within the document's text, and mainly, neither in the objective nor in the methodology, and even less in the results, are not elements that allow this insinuation in the present study. Is the work clearly and accurately presented and does it cite the current literature? Yes Is the study design appropriate and is the work technically sound? Partly Are sufficient details of methods and analysis provided to allow replication by others? Yes If applicable, is the statistical analysis and its interpretation appropriate? Yes Are all the source data underlying the results available to ensure full reproducibility? Yes Are the conclusions drawn adequately supported by the results? Yes Competing Interests: No competing interests were disclosed. Reviewer Expertise: Immunology and Immunogenetics, microbiology. I confirm that I have read this submission and believe that I have an appropriate level of expertise to confirm that it is of an acceptable scientific standard, however I have significant reservations, as outlined above. Close READ LESS CITE CITE HOW TO CITE THIS REPORT Torres Garcia O. Reviewer Report For: Cytotoxicity of Peruvian propolis and Psidium guajava on human gingival fibroblasts, PBMCs and HeLa cells [version 2; peer review: 2 approved] . F1000Research 2022, 11 :430 ( https://doi.org/10.5256/f1000research.121947.r144832 ) The direct URL for this report is: https://f1000research.com/articles/11-430/v1#referee-response-144832 NOTE: it is important to ensure the information in square brackets after the title is included in all citations of this article. COPY CITATION DETAILS Report a concern Author Response 24 Aug 2022 Pablo Millones Gómez , Escuela de Medicina Humana, Universidad Señor de Sipán, Chiclayo, 14000, Peru 24 Aug 2022 Author Response In the study methodology, the authors use the HELA cell lines, human gingival fibroblasts (HGF-1), and peripheral blood mononuclear cells (PBMC); however, the reason for their selection they ... Continue reading In the study methodology, the authors use the HELA cell lines, human gingival fibroblasts (HGF-1), and peripheral blood mononuclear cells (PBMC); however, the reason for their selection they not mentioned in the text. I suggest the authors include some selection criteria for these cell lines used in work. We appreciate the reviewer's comments, the requested criteria were added to the methodology section. To determine the cytotoxicity, the authors use the ethanolic extracts of propolis and P. guajava at the concentration range of 0.024 to 50 mg/mL. What were the selection criteria for this range of concentrations? We appreciate the reviewer's comments, the requested information has been added. Although the document achieves the objective of determining the highest concentrations of the two ethanolic extracts at which cell viabilities more significant than 90% are observed, it would also be interesting to calculate the IC50 of each extract on each cell line. We appreciate the reviewer's comments. Although the IC50 is used in many cytotoxicity studies, the authors decided to use the criteria of Poggio et al. because we seek to continue with studies in the oral cavity. In the last paragraph of the discussion, the authors state that their preliminary data suggest that both products could have a modulating action on the immune response. I suggest rethinking this inference because within the document's text, and mainly, neither in the objective nor in the methodology, and even less in the results, are not elements that allow this insinuation in the present study. We appreciate the reviewer's comments. Changes were made within the last paragraph of the discussion. In the study methodology, the authors use the HELA cell lines, human gingival fibroblasts (HGF-1), and peripheral blood mononuclear cells (PBMC); however, the reason for their selection they not mentioned in the text. I suggest the authors include some selection criteria for these cell lines used in work. We appreciate the reviewer's comments, the requested criteria were added to the methodology section. To determine the cytotoxicity, the authors use the ethanolic extracts of propolis and P. guajava at the concentration range of 0.024 to 50 mg/mL. What were the selection criteria for this range of concentrations? We appreciate the reviewer's comments, the requested information has been added. Although the document achieves the objective of determining the highest concentrations of the two ethanolic extracts at which cell viabilities more significant than 90% are observed, it would also be interesting to calculate the IC50 of each extract on each cell line. We appreciate the reviewer's comments. Although the IC50 is used in many cytotoxicity studies, the authors decided to use the criteria of Poggio et al. because we seek to continue with studies in the oral cavity. In the last paragraph of the discussion, the authors state that their preliminary data suggest that both products could have a modulating action on the immune response. I suggest rethinking this inference because within the document's text, and mainly, neither in the objective nor in the methodology, and even less in the results, are not elements that allow this insinuation in the present study. We appreciate the reviewer's comments. Changes were made within the last paragraph of the discussion. Competing Interests: No competing interests were disclosed. Close Report a concern Respond or Comment COMMENTS ON THIS REPORT Author Response 24 Aug 2022 Pablo Millones Gómez , Escuela de Medicina Humana, Universidad Señor de Sipán, Chiclayo, 14000, Peru 24 Aug 2022 Author Response In the study methodology, the authors use the HELA cell lines, human gingival fibroblasts (HGF-1), and peripheral blood mononuclear cells (PBMC); however, the reason for their selection they ... Continue reading In the study methodology, the authors use the HELA cell lines, human gingival fibroblasts (HGF-1), and peripheral blood mononuclear cells (PBMC); however, the reason for their selection they not mentioned in the text. I suggest the authors include some selection criteria for these cell lines used in work. We appreciate the reviewer's comments, the requested criteria were added to the methodology section. To determine the cytotoxicity, the authors use the ethanolic extracts of propolis and P. guajava at the concentration range of 0.024 to 50 mg/mL. What were the selection criteria for this range of concentrations? We appreciate the reviewer's comments, the requested information has been added. Although the document achieves the objective of determining the highest concentrations of the two ethanolic extracts at which cell viabilities more significant than 90% are observed, it would also be interesting to calculate the IC50 of each extract on each cell line. We appreciate the reviewer's comments. Although the IC50 is used in many cytotoxicity studies, the authors decided to use the criteria of Poggio et al. because we seek to continue with studies in the oral cavity. In the last paragraph of the discussion, the authors state that their preliminary data suggest that both products could have a modulating action on the immune response. I suggest rethinking this inference because within the document's text, and mainly, neither in the objective nor in the methodology, and even less in the results, are not elements that allow this insinuation in the present study. We appreciate the reviewer's comments. Changes were made within the last paragraph of the discussion. In the study methodology, the authors use the HELA cell lines, human gingival fibroblasts (HGF-1), and peripheral blood mononuclear cells (PBMC); however, the reason for their selection they not mentioned in the text. I suggest the authors include some selection criteria for these cell lines used in work. We appreciate the reviewer's comments, the requested criteria were added to the methodology section. To determine the cytotoxicity, the authors use the ethanolic extracts of propolis and P. guajava at the concentration range of 0.024 to 50 mg/mL. What were the selection criteria for this range of concentrations? We appreciate the reviewer's comments, the requested information has been added. Although the document achieves the objective of determining the highest concentrations of the two ethanolic extracts at which cell viabilities more significant than 90% are observed, it would also be interesting to calculate the IC50 of each extract on each cell line. We appreciate the reviewer's comments. Although the IC50 is used in many cytotoxicity studies, the authors decided to use the criteria of Poggio et al. because we seek to continue with studies in the oral cavity. In the last paragraph of the discussion, the authors state that their preliminary data suggest that both products could have a modulating action on the immune response. I suggest rethinking this inference because within the document's text, and mainly, neither in the objective nor in the methodology, and even less in the results, are not elements that allow this insinuation in the present study. We appreciate the reviewer's comments. Changes were made within the last paragraph of the discussion. Competing Interests: No competing interests were disclosed. Close Report a concern COMMENT ON THIS REPORT Views 0 Cite How to cite this report: Rosales Cifuentes TV. Reviewer Report For: Cytotoxicity of Peruvian propolis and Psidium guajava on human gingival fibroblasts, PBMCs and HeLa cells [version 2; peer review: 2 approved] . F1000Research 2022, 11 :430 ( https://doi.org/10.5256/f1000research.121947.r135716 ) The direct URL for this report is: https://f1000research.com/articles/11-430/v1#referee-response-135716 NOTE: it is important to ensure the information in square brackets after the title is included in this citation. Close Copy Citation Details Reviewer Report 23 May 2022 Tania Valentina Rosales Cifuentes , Universidad Nacional Mayor de San Marcos, Lima, Peru Approved VIEWS 0 https://doi.org/10.5256/f1000research.121947.r135716 Title: Its title is adequate, it consigns the most interesting part of the study referred to the cytotoxicity of propolis, using scientific terms keeping the coherence and sufficiency of the article's content. Summary: Although it is true that natural ... Continue reading READ ALL Title: Its title is adequate, it consigns the most interesting part of the study referred to the cytotoxicity of propolis, using scientific terms keeping the coherence and sufficiency of the article's content. Summary: Although it is true that natural products are beneficial, there are few studies that mention the safety of the clinical use of the product. The article emphasizes the importance of knowledge of cell toxicity with a clear and precise explanation of the adequate concentration to avoid the cytotoxic effect. Content: The methods section is well explained, the cytotoxic assay of this study establishes which was the adequate procedure that provided the percentage of safe and reliable cell viability, being coherent with the results in Figure 1. I consider that establishing the detailed procedure as it is done in this study is important so that it is reproducible in future studies of regenerative medicine or tissue engineering. Introduction: I appreciate the effort of the background search and its respective analysis, the introduction is very good, it makes a brief summary of the background making reference to the relevant medical literature, in the last paragraph it mentions the safety of the use of propolis without deterioration of the cells, I suggest, so that the reader can make a comparison and as interesting reading, to cite references 27 and 30, which are similar studies. Results: The results of this study are accurate, achieving the objective to avoid cytotoxicity in human cell lines, recognizing that the effect is dose-dependent. Based on the experience of the researchers evidenced in previous publications, it can be observed that a line of research continues in search of new active principles that can offer a promising pharmacological product based on natural products. Although it is true that this is a preliminary study as indicated by the authors, it is important to point out that future research could consider procedures based on flow cytometry to corroborate the results obtained in the present study. Discussion: The background information showed the therapeutic benefits of natural products, with propolis standing out from the two products, for its antimicrobial, anti-inflammatory and antioxidant effects, among others; however, complementary studies are required to verify its safety in clinical use. The article specifies its strengths and limitations by mentioning which is the ideal concentration to avoid cytotoxicity and that the extracts of Peruvian propolis and guava at lower concentrations can work safely in the fibroblast cell line; however, the authors recognize that since this is an in vitro assay, this value may vary if other types of cell lines are used, making a discussion with other studies carried out; they also suggest that both products could present a modulating action on the immune response, opening perspectives for future research in this field. Is the work clearly and accurately presented and does it cite the current literature? Yes Is the study design appropriate and is the work technically sound? Yes Are sufficient details of methods and analysis provided to allow replication by others? Yes If applicable, is the statistical analysis and its interpretation appropriate? Yes Are all the source data underlying the results available to ensure full reproducibility? Yes Are the conclusions drawn adequately supported by the results? Yes Competing Interests: No competing interests were disclosed. Reviewer Expertise: Natural products and microbiology I confirm that I have read this submission and believe that I have an appropriate level of expertise to confirm that it is of an acceptable scientific standard. Close READ LESS CITE CITE HOW TO CITE THIS REPORT Rosales Cifuentes TV. Reviewer Report For: Cytotoxicity of Peruvian propolis and Psidium guajava on human gingival fibroblasts, PBMCs and HeLa cells [version 2; peer review: 2 approved] . F1000Research 2022, 11 :430 ( https://doi.org/10.5256/f1000research.121947.r135716 ) The direct URL for this report is: https://f1000research.com/articles/11-430/v1#referee-response-135716 NOTE: it is important to ensure the information in square brackets after the title is included in all citations of this article. COPY CITATION DETAILS Report a concern Respond or Comment COMMENT ON THIS REPORT Comments on this article Comments (0) Version 2 VERSION 2 PUBLISHED 19 Apr 2022 ADD YOUR COMMENT Comment keyboard_arrow_left keyboard_arrow_right Open Peer Review Reviewer Status info_outline Alongside their report, reviewers assign a status to the article: Approved The paper is scientifically sound in its current form and only minor, if any, improvements are suggested Approved with reservations A number of small changes, sometimes more significant revisions are required to address specific details and improve the papers academic merit. Not approved Fundamental flaws in the paper seriously undermine the findings and conclusions Reviewer Reports Invited Reviewers 1 2 Version 2 (revision) 16 Aug 22 read read Version 1 19 Apr 22 read read Tania Valentina Rosales Cifuentes , Universidad Nacional Mayor de San Marcos, Lima, Peru Orlando Torres Garcia , Universidad Antonio Nariño, Bogotá, Colombia; Javeriana University, Bogotá, Colombia Comments on this article All Comments (0) Add a comment Sign up for content alerts Sign Up You are now signed up to receive this alert Browse by related subjects keyboard_arrow_left Back to all reports Reviewer Report 0 Views copyright © 2022 Torres Garcia O. This is an open access peer review report distributed under the terms of the Creative Commons Attribution License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. 17 Aug 2022 | for Version 2 Orlando Torres Garcia , Faculty of Veterinary Medicine and Zootechnics, Universidad Antonio Nariño, Bogotá, Colombia; Faculty of Sciences, Javeriana University, Bogotá, Colombia 0 Views copyright © 2022 Torres Garcia O. This is an open access peer review report distributed under the terms of the Creative Commons Attribution License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. format_quote Cite this report speaker_notes Responses (0) Approved info_outline Alongside their report, reviewers assign a status to the article: Approved The paper is scientifically sound in its current form and only minor, if any, improvements are suggested Approved with reservations A number of small changes, sometimes more significant revisions are required to address specific details and improve the papers academic merit. Not approved Fundamental flaws in the paper seriously undermine the findings and conclusions The document is interesting and presents two compounds of natural origin (Propolis and Psidium guajava) with potential for medical and dental application. Competing Interests No competing interests were disclosed. Reviewer Expertise Immunology and Immunogenetics, microbiology. I confirm that I have read this submission and believe that I have an appropriate level of expertise to confirm that it is of an acceptable scientific standard. reply Respond to this report Responses (0) Torres Garcia O. Peer Review Report For: Cytotoxicity of Peruvian propolis and Psidium guajava on human gingival fibroblasts, PBMCs and HeLa cells [version 2; peer review: 2 approved] . F1000Research 2022, 11 :430 ( https://doi.org/10.5256/f1000research.137410.r147645) NOTE: it is important to ensure the information in square brackets after the title is included in this citation. The direct URL for this report is: https://f1000research.com/articles/11-430/v2#referee-response-147645 keyboard_arrow_left Back to all reports Reviewer Report 0 Views copyright © 2022 Rosales Cifuentes T. This is an open access peer review report distributed under the terms of the Creative Commons Attribution License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. 17 Aug 2022 | for Version 2 Tania Valentina Rosales Cifuentes , Universidad Nacional Mayor de San Marcos, Lima, Peru 0 Views copyright © 2022 Rosales Cifuentes T. This is an open access peer review report distributed under the terms of the Creative Commons Attribution License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. format_quote Cite this report speaker_notes Responses (0) Approved info_outline Alongside their report, reviewers assign a status to the article: Approved The paper is scientifically sound in its current form and only minor, if any, improvements are suggested Approved with reservations A number of small changes, sometimes more significant revisions are required to address specific details and improve the papers academic merit. Not approved Fundamental flaws in the paper seriously undermine the findings and conclusions Competing Interests No competing interests were disclosed. Reviewer Expertise Natural products and microbiology I confirm that I have read this submission and believe that I have an appropriate level of expertise to confirm that it is of an acceptable scientific standard. reply Respond to this report Responses (0) Rosales Cifuentes TV. Peer Review Report For: Cytotoxicity of Peruvian propolis and Psidium guajava on human gingival fibroblasts, PBMCs and HeLa cells [version 2; peer review: 2 approved] . F1000Research 2022, 11 :430 ( https://doi.org/10.5256/f1000research.137410.r147646) NOTE: it is important to ensure the information in square brackets after the title is included in this citation. The direct URL for this report is: https://f1000research.com/articles/11-430/v2#referee-response-147646 keyboard_arrow_left Back to all reports Reviewer Report 0 Views copyright © 2022 Torres Garcia O. This is an open access peer review report distributed under the terms of the Creative Commons Attribution License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. 09 Aug 2022 | for Version 1 Orlando Torres Garcia , Faculty of Veterinary Medicine and Zootechnics, Universidad Antonio Nariño, Bogotá, Colombia; Faculty of Sciences, Javeriana University, Bogotá, Colombia 0 Views copyright © 2022 Torres Garcia O. This is an open access peer review report distributed under the terms of the Creative Commons Attribution License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. format_quote Cite this report speaker_notes Responses (1) Approved With Reservations info_outline Alongside their report, reviewers assign a status to the article: Approved The paper is scientifically sound in its current form and only minor, if any, improvements are suggested Approved with reservations A number of small changes, sometimes more significant revisions are required to address specific details and improve the papers academic merit. Not approved Fundamental flaws in the paper seriously undermine the findings and conclusions In this article, the authors evaluated the cytotoxic effect of ethanolic extracts of propolis and P. guajava on HELA cell lines, human gingival fibroblasts (HGF-1), and peripheral blood mononuclear cells (PBMC), finding cell viability more significant than 90% at concentrations equal to or less than 0.24 mg/mL and 0.024 mg/mL for EEG and EPP, respectively. Within this perspective, the authors infer potential applications in medicine and dentistry. Main comments: In the study methodology, the authors use the HELA cell lines, human gingival fibroblasts (HGF-1), and peripheral blood mononuclear cells (PBMC); however, the reason for their selection they not mentioned in the text. I suggest the authors include some selection criteria for these cell lines used in work. To determine the cytotoxicity, the authors use the ethanolic extracts of propolis and P. guajava at the concentration range of 0.024 to 50 mg/mL. What were the selection criteria for this range of concentrations? Although the document achieves the objective of determining the highest concentrations of the two ethanolic extracts at which cell viabilities more significant than 90% are observed, it would also be interesting to calculate the IC50 of each extract on each cell line. Minor comment: In the last paragraph of the discussion, the authors state that their preliminary data suggest that both products could have a modulating action on the immune response. I suggest rethinking this inference because within the document's text, and mainly, neither in the objective nor in the methodology, and even less in the results, are not elements that allow this insinuation in the present study. Is the work clearly and accurately presented and does it cite the current literature? Yes Is the study design appropriate and is the work technically sound? Partly Are sufficient details of methods and analysis provided to allow replication by others? Yes If applicable, is the statistical analysis and its interpretation appropriate? Yes Are all the source data underlying the results available to ensure full reproducibility? Yes Are the conclusions drawn adequately supported by the results? Yes Competing Interests No competing interests were disclosed. Reviewer Expertise Immunology and Immunogenetics, microbiology. I confirm that I have read this submission and believe that I have an appropriate level of expertise to confirm that it is of an acceptable scientific standard, however I have significant reservations, as outlined above. reply Respond to this report Responses (1) Author Response 24 Aug 2022 Pablo Millones Gómez, Escuela de Medicina Humana, Universidad Señor de Sipán, Chiclayo, 14000, Peru In the study methodology, the authors use the HELA cell lines, human gingival fibroblasts (HGF-1), and peripheral blood mononuclear cells (PBMC); however, the reason for their selection they not mentioned in the text. I suggest the authors include some selection criteria for these cell lines used in work. We appreciate the reviewer's comments, the requested criteria were added to the methodology section. To determine the cytotoxicity, the authors use the ethanolic extracts of propolis and P. guajava at the concentration range of 0.024 to 50 mg/mL. What were the selection criteria for this range of concentrations? We appreciate the reviewer's comments, the requested information has been added. Although the document achieves the objective of determining the highest concentrations of the two ethanolic extracts at which cell viabilities more significant than 90% are observed, it would also be interesting to calculate the IC50 of each extract on each cell line. We appreciate the reviewer's comments. Although the IC50 is used in many cytotoxicity studies, the authors decided to use the criteria of Poggio et al. because we seek to continue with studies in the oral cavity. In the last paragraph of the discussion, the authors state that their preliminary data suggest that both products could have a modulating action on the immune response. I suggest rethinking this inference because within the document's text, and mainly, neither in the objective nor in the methodology, and even less in the results, are not elements that allow this insinuation in the present study. We appreciate the reviewer's comments. Changes were made within the last paragraph of the discussion. View more View less Competing Interests No competing interests were disclosed. reply Respond Report a concern Torres Garcia O. Peer Review Report For: Cytotoxicity of Peruvian propolis and Psidium guajava on human gingival fibroblasts, PBMCs and HeLa cells [version 2; peer review: 2 approved] . F1000Research 2022, 11 :430 ( https://doi.org/10.5256/f1000research.121947.r144832) NOTE: it is important to ensure the information in square brackets after the title is included in this citation. The direct URL for this report is: https://f1000research.com/articles/11-430/v1#referee-response-144832 keyboard_arrow_left Back to all reports Reviewer Report 0 Views copyright © 2022 Rosales Cifuentes T. This is an open access peer review report distributed under the terms of the Creative Commons Attribution License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. 23 May 2022 | for Version 1 Tania Valentina Rosales Cifuentes , Universidad Nacional Mayor de San Marcos, Lima, Peru 0 Views copyright © 2022 Rosales Cifuentes T. This is an open access peer review report distributed under the terms of the Creative Commons Attribution License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. format_quote Cite this report speaker_notes Responses (0) Approved info_outline Alongside their report, reviewers assign a status to the article: Approved The paper is scientifically sound in its current form and only minor, if any, improvements are suggested Approved with reservations A number of small changes, sometimes more significant revisions are required to address specific details and improve the papers academic merit. Not approved Fundamental flaws in the paper seriously undermine the findings and conclusions Title: Its title is adequate, it consigns the most interesting part of the study referred to the cytotoxicity of propolis, using scientific terms keeping the coherence and sufficiency of the article's content. Summary: Although it is true that natural products are beneficial, there are few studies that mention the safety of the clinical use of the product. The article emphasizes the importance of knowledge of cell toxicity with a clear and precise explanation of the adequate concentration to avoid the cytotoxic effect. Content: The methods section is well explained, the cytotoxic assay of this study establishes which was the adequate procedure that provided the percentage of safe and reliable cell viability, being coherent with the results in Figure 1. I consider that establishing the detailed procedure as it is done in this study is important so that it is reproducible in future studies of regenerative medicine or tissue engineering. Introduction: I appreciate the effort of the background search and its respective analysis, the introduction is very good, it makes a brief summary of the background making reference to the relevant medical literature, in the last paragraph it mentions the safety of the use of propolis without deterioration of the cells, I suggest, so that the reader can make a comparison and as interesting reading, to cite references 27 and 30, which are similar studies. Results: The results of this study are accurate, achieving the objective to avoid cytotoxicity in human cell lines, recognizing that the effect is dose-dependent. Based on the experience of the researchers evidenced in previous publications, it can be observed that a line of research continues in search of new active principles that can offer a promising pharmacological product based on natural products. Although it is true that this is a preliminary study as indicated by the authors, it is important to point out that future research could consider procedures based on flow cytometry to corroborate the results obtained in the present study. Discussion: The background information showed the therapeutic benefits of natural products, with propolis standing out from the two products, for its antimicrobial, anti-inflammatory and antioxidant effects, among others; however, complementary studies are required to verify its safety in clinical use. The article specifies its strengths and limitations by mentioning which is the ideal concentration to avoid cytotoxicity and that the extracts of Peruvian propolis and guava at lower concentrations can work safely in the fibroblast cell line; however, the authors recognize that since this is an in vitro assay, this value may vary if other types of cell lines are used, making a discussion with other studies carried out; they also suggest that both products could present a modulating action on the immune response, opening perspectives for future research in this field. Is the work clearly and accurately presented and does it cite the current literature? Yes Is the study design appropriate and is the work technically sound? Yes Are sufficient details of methods and analysis provided to allow replication by others? Yes If applicable, is the statistical analysis and its interpretation appropriate? Yes Are all the source data underlying the results available to ensure full reproducibility? Yes Are the conclusions drawn adequately supported by the results? Yes Competing Interests No competing interests were disclosed. Reviewer Expertise Natural products and microbiology I confirm that I have read this submission and believe that I have an appropriate level of expertise to confirm that it is of an acceptable scientific standard. reply Respond to this report Responses (0) Rosales Cifuentes TV. Peer Review Report For: Cytotoxicity of Peruvian propolis and Psidium guajava on human gingival fibroblasts, PBMCs and HeLa cells [version 2; peer review: 2 approved] . F1000Research 2022, 11 :430 ( https://doi.org/10.5256/f1000research.121947.r135716) NOTE: it is important to ensure the information in square brackets after the title is included in this citation. The direct URL for this report is: https://f1000research.com/articles/11-430/v1#referee-response-135716 Alongside their report, reviewers assign a status to the article: Approved - the paper is scientifically sound in its current form and only minor, if any, improvements are suggested Approved with reservations - A number of small changes, sometimes more significant revisions are required to address specific details and improve the papers academic merit. Not approved - fundamental flaws in the paper seriously undermine the findings and conclusions Adjust parameters to alter display View on desktop for interactive features Includes Interactive Elements View on desktop for interactive features Competing Interests Policy Provide sufficient details of any financial or non-financial competing interests to enable users to assess whether your comments might lead a reasonable person to question your impartiality. 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