Antibiofilm Activity of Lactic Acid Bacteria of Probiotic Candidates Against Pathogenic Bacteria Streptococcus mutans and Enterococcus faecalis

Antibiofilm Activity of Lactic Acid Bacteria of Probiotic Candidates Against Pathogenic Bacteria Streptococcus mutans and Enterococcus faecalis | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Antibiofilm Activity of Lactic Acid Bacteria of Probiotic Candidates Against Pathogenic Bacteria Streptococcus mutans and Enterococcus faecalis Chrismelan Julianto Pesoa, Ema Damayanti, Nurfazila Di’a Rifannisaa, and 2 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-8242997/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Background Streptococcus mutans and Enterococcus faecalis are Gram-positive bacteria that play a role in the development of dental caries. These bacteria can form biofilm structures that inhibit the immune system and cause Multi-Drug Resistance. Lactic acid bacteria (LAB) produce organic acid compounds with potential probiotic. Methods In this study, Cell-Free Culture Supernatant (CFCS) LAB were derived from stingless honey bees with the following codes: Lacticaseibacillus rhamnosus strain TB-3 (PQ386429), L. rhamnosus strain HI-1 (PQ386478), Pediococcus acidilactici strain HI-5 (PQ386480), and P. acidilactici strain LT-3 (PQ386496). LAB compounds were tested for antibiofilm and antibacterial activity based on the Minimum Inhibitory Concentration (MIC), Minimum Bactericidal Concentration (MBC), and in silico studies. Results CFCS TB-3 and LT-3 showed MIC values of 50% (v/v) against S. Mutans and 60% (v/v) against E. faecalis . TB-3 and LT-3 had MBC values ​​at higher concentrations than the other strains and had the highest antibiofilm activity at a concentration of 60% (v/v) against S. Mutans and 50% (v/v) against E. faecalis . In contrast, LAB metabolite compounds have a good affinity for the target protein that causes biofilms. Conclusion These results indicate that TB-3 and LT-3 are effective antibacterial and antibiofilm agents. Lactic acid bacteria Streptococcus mutans Enterococcus faecalis Antibiofilm Antibacterial Molecular Docking Full Text Additional Declarations No competing interests reported. Table 1 is not available with this version Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-8242997","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":557068455,"identity":"fd920027-afdb-482f-9c8d-7d73638fa1b8","order_by":0,"name":"Chrismelan Julianto Pesoa","email":"","orcid":"","institution":"Gadjah Mada University","correspondingAuthor":false,"prefix":"","firstName":"Chrismelan","middleName":"Julianto","lastName":"Pesoa","suffix":""},{"id":557068456,"identity":"d5f370fb-7a6f-4671-b102-392f97a66fbe","order_by":1,"name":"Ema Damayanti","email":"","orcid":"","institution":"National Research and Innovation 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These bacteria can form biofilm structures that inhibit the immune system and cause Multi-Drug Resistance. Lactic acid bacteria (LAB) produce organic acid compounds with potential probiotic.\u003c/p\u003e\u003ch2\u003eMethods\u003c/h2\u003e\u003cp\u003eIn this study, Cell-Free Culture Supernatant (CFCS) LAB were derived from stingless honey bees with the following codes: \u003cem\u003eLacticaseibacillus rhamnosus\u003c/em\u003e strain TB-3 (PQ386429), \u003cem\u003eL. rhamnosus\u003c/em\u003e strain HI-1 (PQ386478), \u003cem\u003ePediococcus acidilactici\u003c/em\u003e strain HI-5 (PQ386480), and \u003cem\u003eP. acidilactici\u003c/em\u003e strain LT-3 (PQ386496). LAB compounds were tested for antibiofilm and antibacterial activity based on the Minimum Inhibitory Concentration (MIC), Minimum Bactericidal Concentration (MBC), and in silico studies.\u003c/p\u003e\u003ch2\u003eResults\u003c/h2\u003e\u003cp\u003eCFCS TB-3 and LT-3 showed MIC values of 50% (v/v) against \u003cem\u003eS. Mutans\u003c/em\u003e and 60% (v/v) against \u003cem\u003eE. faecalis\u003c/em\u003e. TB-3 and LT-3 had MBC values ​​at higher concentrations than the other strains and had the highest antibiofilm activity at a concentration of 60% (v/v) against \u003cem\u003eS. Mutans\u003c/em\u003e and 50% (v/v) against \u003cem\u003eE. faecalis\u003c/em\u003e. In contrast, LAB metabolite compounds have a good affinity for the target protein that causes biofilms.\u003c/p\u003e\u003ch2\u003eConclusion\u003c/h2\u003e\u003cp\u003eThese results indicate that TB-3 and LT-3 are effective antibacterial and antibiofilm agents.\u003c/p\u003e","manuscriptTitle":"Antibiofilm Activity of Lactic Acid Bacteria of Probiotic Candidates Against Pathogenic Bacteria Streptococcus mutans and Enterococcus faecalis","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2025-12-11 22:39:21","doi":"10.21203/rs.3.rs-8242997/v1","editorialEvents":[{"type":"communityComments","content":0}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"a27cfbdc-1e8f-4c0a-8c70-deb3e306b061","owner":[],"postedDate":"December 11th, 2025","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"posted","subjectAreas":[],"tags":[],"updatedAt":"2026-01-30T05:09:14+00:00","versionOfRecord":[],"versionCreatedAt":"2025-12-11 22:39:21","video":"","vorDoi":"","vorDoiUrl":"","workflowStages":[]},"version":"v1","identity":"rs-8242997","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-8242997","identity":"rs-8242997","version":["v1"]},"buildId":"8U1c8b4HqxoKbykW_rLl7","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}