Agronomic and physiological responses of seed-potato in different organic substrates

Agronomic and physiological responses of seed-potato in different organic substrates | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Article Agronomic and physiological responses of seed-potato in different organic substrates Ramon Quaresma Zeferino, Amanda de Mello Gonçalves Gaião, Antonio Fernandes Monteiro Filho, and 13 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-7659075/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Potato cultivation is very important, especially in organic systems. However, high cost and low availability of seed-potatoes are limiting factors. This study aimed to formulate and select an organic substrate to promote vegetative and reproductive development for seed-potato production. Four experiments were carried out in a greenhouse, in a completely randomized design with five replicates, with the cultivars (cv.) Aracy, Ágata and Aracy Ruiva, evaluating survival, leaf growth rate, gas exchange, agronomic variables, and macronutrients. Sprouts were used as propagation units. Experiment I was carried out to pre-select the best organic substrates, using cv. Aracy in 16 different substrates (S1, S2, S3, S4, S5, S6, S7, S8, S9, S10, S11, S12, S13, S14, S15, and S16), with survival rates ranging from 80 to 100%. Experiments II, III and IV were carried out with 8 substrates; the second experiment was conducted with the cv. Aracy, the third experiment was conducted with the cv. Ágata and the forth experiment was conducted with the cv. Aracy Ruiva, and compared with commercial substrate (Mecplant®). The survival rates in the experiments were similar or higher than the commercial substrate. The substrates that promoted the highest leaf growth rate were S8 (experiments I and II), S11 (experiment III), and S9 (experiment IV). Substrates S8, S15, S1 and commercial promoted the highest instantaneous efficiency of carboxylation in plants of Aracy. The Aracy Ruiva grown in substrates S15, S8, S2 and commercial had higher internal concentration of CO 2 ; and those grown in S3 had higher instantaneous efficiency of water use. The eight substrates selected provided similar mass, diameter and length of tubers produced by all cultivars, when compared to the commercial substrate. Significance was observed only for Ágata, regarding the number of tubers. Among the organic substrates evaluated, substrate S11 is indicated for the production of seed-potatoes, as it promotes the highest survival among the evaluated substrates, high leaf area development, being the highest to the cv. Ágata, promoted tuberization, increasing the tuber number produced by the plants and the tuber mass, and length and transversal diameter tuber of the evaluated cultivars and the substrate is formulated with washed coconut powder; sifted poultry litter; humus, limestone and sand, products easily to find by growers, with low cost and allowed to use in organic agriculture. Biological sciences/Biotechnology Biological sciences/Plant sciences IAC-Sprout/Seed Potato Technology organic system physiological responses Solanum tuberosum L Full Text Additional Declarations No competing interests reported. Supplementary Files Supplementarymaterial.xlsx Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-7659075","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Article","associatedPublications":[],"authors":[{"id":546361466,"identity":"40509bf1-3136-4c5a-b03a-73faaf96f589","order_by":0,"name":"Ramon Quaresma Zeferino","email":"","orcid":"","institution":"Universidade Estadual da Paraíba","correspondingAuthor":false,"prefix":"","firstName":"Ramon","middleName":"Quaresma","lastName":"Zeferino","suffix":""},{"id":546361468,"identity":"45fceaf1-2b39-40e7-b499-b46136356940","order_by":1,"name":"Amanda de Mello Gonçalves Gaião","email":"","orcid":"","institution":"Universidade Estadual da Paraíba","correspondingAuthor":false,"prefix":"","firstName":"Amanda","middleName":"de Mello Gonçalves","lastName":"Gaião","suffix":""},{"id":546361470,"identity":"b2eebb8b-a257-4a01-9d83-ff8e5b5619c9","order_by":2,"name":"Antonio Fernandes Monteiro Filho","email":"","orcid":"","institution":"Universidade Estadual da Paraíba","correspondingAuthor":false,"prefix":"","firstName":"Antonio","middleName":"Fernandes Monteiro","lastName":"Filho","suffix":""},{"id":546361472,"identity":"3796aac9-305c-4142-ba30-01d191d0b255","order_by":3,"name":"Josely Dantas Fernandes","email":"","orcid":"","institution":"Universidade Estadual da Paraíba","correspondingAuthor":false,"prefix":"","firstName":"Josely","middleName":"Dantas","lastName":"Fernandes","suffix":""},{"id":546361473,"identity":"f58aedc6-e19b-4c9c-8f6f-f04c6cf4b721","order_by":4,"name":"Sayonara Medeiros Duarte","email":"","orcid":"","institution":"Universidade Estadual da Paraíba","correspondingAuthor":false,"prefix":"","firstName":"Sayonara","middleName":"Medeiros","lastName":"Duarte","suffix":""},{"id":546361474,"identity":"9f431039-e83d-49c8-8253-18c66eff092e","order_by":5,"name":"Ricardo Alves de Olinda","email":"","orcid":"","institution":"Universidade Estadual da Paraíba","correspondingAuthor":false,"prefix":"","firstName":"Ricardo","middleName":"Alves","lastName":"de Olinda","suffix":""},{"id":546361475,"identity":"fa9a930f-58d0-4c1c-b35e-a1aab32349ea","order_by":6,"name":"Raquel da Silva Ferreira","email":"","orcid":"","institution":"Universidade Estadual da Paraíba","correspondingAuthor":false,"prefix":"","firstName":"Raquel","middleName":"da Silva","lastName":"Ferreira","suffix":""},{"id":546361476,"identity":"ca446023-2153-4cb8-bceb-c6e1de05dc1f","order_by":7,"name":"Jamille de Lima Rodrigues","email":"","orcid":"","institution":"Universidade Estadual da Paraíba","correspondingAuthor":false,"prefix":"","firstName":"Jamille","middleName":"de Lima","lastName":"Rodrigues","suffix":""},{"id":546361478,"identity":"5b1c31db-4612-4de6-afb6-ba744139d4b7","order_by":8,"name":"Cristiano dos Santos Sousa","email":"","orcid":"","institution":"Universidade Estadual da Paraíba","correspondingAuthor":false,"prefix":"","firstName":"Cristiano","middleName":"dos Santos","lastName":"Sousa","suffix":""},{"id":546361479,"identity":"6e1b8d29-d3b2-4042-8f13-fecdd8bf3b2e","order_by":9,"name":"Franklin Sales de Araújo","email":"","orcid":"","institution":"Universidade Estadual da Paraíba","correspondingAuthor":false,"prefix":"","firstName":"Franklin","middleName":"Sales","lastName":"de Araújo","suffix":""},{"id":546361481,"identity":"ea2d2438-8137-4ac0-8c25-df138ec68c84","order_by":10,"name":"Severino Bezerra de Araújo","email":"","orcid":"","institution":"Universidade Estadual da Paraíba","correspondingAuthor":false,"prefix":"","firstName":"Severino","middleName":"Bezerra","lastName":"de Araújo","suffix":""},{"id":546361484,"identity":"96330c42-7981-4299-8563-f555e697eec3","order_by":11,"name":"Guilherme Felix Dias","email":"","orcid":"","institution":"Universidade Estadual da Paraíba","correspondingAuthor":false,"prefix":"","firstName":"Guilherme","middleName":"Felix","lastName":"Dias","suffix":""},{"id":546361487,"identity":"75432c4b-2b21-4607-8e61-a33e6210a85f","order_by":12,"name":"Semako Ibrahim Bonou","email":"","orcid":"","institution":"Universidade Federal de Campina Grande","correspondingAuthor":false,"prefix":"","firstName":"Semako","middleName":"Ibrahim","lastName":"Bonou","suffix":""},{"id":546361490,"identity":"1e89457a-fa0f-45b1-af19-a726c35ddc50","order_by":13,"name":"Mônica Danielly de Mello Oliveira","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAABBUlEQVRIiWNgGAWjYJCCAwwMzFBmgQUDP4hOKCBOC2MDg4EEg2QDSIsBQYuQtBgcALHxaNFt7z144EeNdbQ5e+/zBx8MJOSNz69O/PDAgEGeX+wAVi1mZ84lHOw5lp67s+e4YeMMAwnDbTfebpYAOsxw5uwE7Fpu5BgcZmA7nLvhRhpjM4+BBOO2G2c3gLQkGNzGp+UfVMsfAwn7zTPObv5BUAtjG1QL0PuJG/h7t+G35cwZg4O9fSC/HGOc2WMgkTzjBu82iwSgp3D65XiP8Ycf36xzt7O3MXz4UWFj299/dvNNIEOeXxq7FjhARIQEWKUEfuWoWvgPEFY9CkbBKBgFIwoAAMumZ8oOhLe/AAAAAElFTkSuQmCC","orcid":"","institution":"Universidade Estadual da Paraíba","correspondingAuthor":true,"prefix":"","firstName":"Mônica","middleName":"Danielly de Mello","lastName":"Oliveira","suffix":""},{"id":546361492,"identity":"d88d3d39-83ff-47ed-95d1-f3562938eaa6","order_by":14,"name":"Alberto Soares de Melo","email":"","orcid":"","institution":"Universidade Estadual da Paraíba","correspondingAuthor":false,"prefix":"","firstName":"Alberto","middleName":"Soares","lastName":"de Melo","suffix":""},{"id":546361494,"identity":"95b0069b-d788-43dd-8bb3-116f31e7d3e7","order_by":15,"name":"Élida Barbosa Corrêa","email":"","orcid":"","institution":"Universidade Estadual da Paraíba","correspondingAuthor":false,"prefix":"","firstName":"Élida","middleName":"Barbosa","lastName":"Corrêa","suffix":""}],"badges":[],"createdAt":"2025-09-19 13:38:20","currentVersionCode":1,"declarations":"","doi":"10.21203/rs.3.rs-7659075/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-7659075/v1","draftVersion":[],"editorialEvents":[],"editorialNote":"","failedWorkflow":false,"files":[{"id":96197515,"identity":"52c70f51-479c-4697-b1c4-798564dace33","added_by":"auto","created_at":"2025-11-18 15:40:27","extension":"docx","order_by":0,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":341570,"visible":true,"origin":"","legend":"","description":"","filename":"Figures.25.docx","url":"https://assets-eu.researchsquare.com/files/rs-7659075/v1/c4893d80270932d8659927e9.docx"},{"id":96197526,"identity":"70584496-e412-48f2-8174-8932e86489fa","added_by":"auto","created_at":"2025-11-18 15:40:28","extension":"docx","order_by":1,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":337683,"visible":true,"origin":"","legend":"","description":"","filename":"Organicsubstratesforseedpotatoproduction.docx","url":"https://assets-eu.researchsquare.com/files/rs-7659075/v1/a157773600ee9ff816bbaf1c.docx"},{"id":96197521,"identity":"d7583c6a-a391-49f0-8ecd-50fb8e425f98","added_by":"auto","created_at":"2025-11-18 15:40:28","extension":"docx","order_by":2,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":66909,"visible":true,"origin":"","legend":"","description":"","filename":"Tables.25.docx","url":"https://assets-eu.researchsquare.com/files/rs-7659075/v1/de6aa5a770b01a341a51e62b.docx"},{"id":96197524,"identity":"cf36c7b9-d463-4f6d-863b-0ee82d2cc4b6","added_by":"auto","created_at":"2025-11-18 15:40:28","extension":"json","order_by":3,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":16483,"visible":true,"origin":"","legend":"","description":"","filename":"d0acba5ab5924e46b32bc5abcabc6fbf.json","url":"https://assets-eu.researchsquare.com/files/rs-7659075/v1/9c774fef42c50ff3ca43224f.json"},{"id":96197525,"identity":"b84f007c-dcd0-4cda-9dac-ba5572870ec6","added_by":"auto","created_at":"2025-11-18 15:40:28","extension":"xlsx","order_by":4,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":110692,"visible":true,"origin":"","legend":"","description":"","filename":"Supplementarymaterial.xlsx","url":"https://assets-eu.researchsquare.com/files/rs-7659075/v1/90c9362d480b970bc1c3af9e.xlsx"},{"id":96252031,"identity":"4db658b1-3223-4e95-9661-d3fdc1f173cd","added_by":"auto","created_at":"2025-11-19 07:40:21","extension":"xml","order_by":5,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":248112,"visible":true,"origin":"","legend":"","description":"","filename":"d0acba5ab5924e46b32bc5abcabc6fbf1enriched.xml","url":"https://assets-eu.researchsquare.com/files/rs-7659075/v1/a1dc33f1a936562236a338e8.xml"},{"id":96197529,"identity":"1c3cfc1f-de8d-4445-af94-308305462a69","added_by":"auto","created_at":"2025-11-18 15:40:28","extension":"jpeg","order_by":6,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":563512,"visible":true,"origin":"","legend":"","description":"","filename":"floatimage1.jpeg","url":"https://assets-eu.researchsquare.com/files/rs-7659075/v1/42db8fd96957d7bb30fa2f77.jpeg"},{"id":96197532,"identity":"611894b4-5deb-4ff9-940c-1a255a4eb8b9","added_by":"auto","created_at":"2025-11-18 15:40:28","extension":"jpeg","order_by":7,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":563512,"visible":true,"origin":"","legend":"","description":"","filename":"floatimage1.jpeg","url":"https://assets-eu.researchsquare.com/files/rs-7659075/v1/893bd4acdf5752851818c9e1.jpeg"},{"id":96252794,"identity":"09a11b70-aa8f-4065-ac0c-f323d115067d","added_by":"auto","created_at":"2025-11-19 07:41:29","extension":"jpeg","order_by":8,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":314313,"visible":true,"origin":"","legend":"","description":"","filename":"floatimage11.jpeg","url":"https://assets-eu.researchsquare.com/files/rs-7659075/v1/009490e5bc462fc4f48bdc0d.jpeg"},{"id":96197518,"identity":"6a6f225c-6f88-4bff-9209-154466a7e238","added_by":"auto","created_at":"2025-11-18 15:40:28","extension":"jpeg","order_by":9,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":1074,"visible":true,"origin":"","legend":"","description":"","filename":"floatimage12.jpeg","url":"https://assets-eu.researchsquare.com/files/rs-7659075/v1/dd804dc38fd676c77274edb6.jpeg"},{"id":96251257,"identity":"3a3ec50b-e43f-4a2e-b70b-e15bd1a98dd2","added_by":"auto","created_at":"2025-11-19 07:39:34","extension":"jpeg","order_by":10,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":125945,"visible":true,"origin":"","legend":"","description":"","filename":"floatimage13.jpeg","url":"https://assets-eu.researchsquare.com/files/rs-7659075/v1/a06759f9d9684cb66129275f.jpeg"},{"id":96251336,"identity":"9dab013b-39dc-4121-bec0-c4c1ae0a66ce","added_by":"auto","created_at":"2025-11-19 07:39:39","extension":"jpeg","order_by":11,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":121512,"visible":true,"origin":"","legend":"","description":"","filename":"floatimage14.jpeg","url":"https://assets-eu.researchsquare.com/files/rs-7659075/v1/e759432a51a4dea11257b980.jpeg"},{"id":96252673,"identity":"2fadd99a-7241-4781-a61d-acafa7ee7ff0","added_by":"auto","created_at":"2025-11-19 07:41:20","extension":"jpeg","order_by":12,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":168113,"visible":true,"origin":"","legend":"","description":"","filename":"floatimage15.jpeg","url":"https://assets-eu.researchsquare.com/files/rs-7659075/v1/52fce858089efe6dbae1890c.jpeg"},{"id":96197522,"identity":"d41ea6a7-cb97-4982-82e2-5212118b92d1","added_by":"auto","created_at":"2025-11-18 15:40:28","extension":"jpeg","order_by":13,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":176918,"visible":true,"origin":"","legend":"","description":"","filename":"floatimage16.jpeg","url":"https://assets-eu.researchsquare.com/files/rs-7659075/v1/251fa334cbb73d9dedd27548.jpeg"},{"id":96252092,"identity":"90d85923-363c-4243-bd8b-3c8a7a2da12c","added_by":"auto","created_at":"2025-11-19 07:40:26","extension":"jpeg","order_by":14,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":189275,"visible":true,"origin":"","legend":"","description":"","filename":"floatimage17.jpeg","url":"https://assets-eu.researchsquare.com/files/rs-7659075/v1/44a9c6252210d27857731d63.jpeg"},{"id":96252614,"identity":"c5dd04d1-5755-4568-8e5b-8008bbecc319","added_by":"auto","created_at":"2025-11-19 07:41:16","extension":"jpeg","order_by":15,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":135711,"visible":true,"origin":"","legend":"","description":"","filename":"floatimage18.jpeg","url":"https://assets-eu.researchsquare.com/files/rs-7659075/v1/bcb3b721f16f84b97e3484b7.jpeg"},{"id":96197536,"identity":"ec8b8434-e3e6-4458-813b-7f8dcda5a5b2","added_by":"auto","created_at":"2025-11-18 15:40:28","extension":"jpeg","order_by":16,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":314313,"visible":true,"origin":"","legend":"","description":"","filename":"floatimage11.jpeg","url":"https://assets-eu.researchsquare.com/files/rs-7659075/v1/ee20addc729352926f1b8c50.jpeg"},{"id":96252939,"identity":"97ed43ca-f037-4e72-aca4-2f83f795624a","added_by":"auto","created_at":"2025-11-19 07:41:41","extension":"jpeg","order_by":17,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":1074,"visible":true,"origin":"","legend":"","description":"","filename":"floatimage12.jpeg","url":"https://assets-eu.researchsquare.com/files/rs-7659075/v1/1393d1623f2dff9d1cb4ba97.jpeg"},{"id":96197543,"identity":"eb4a8159-91af-4a27-bac9-bca722ce71c7","added_by":"auto","created_at":"2025-11-18 15:40:28","extension":"jpeg","order_by":18,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":125945,"visible":true,"origin":"","legend":"","description":"","filename":"floatimage13.jpeg","url":"https://assets-eu.researchsquare.com/files/rs-7659075/v1/f076c039f7a9f3dabe938808.jpeg"},{"id":96197544,"identity":"332b6ef7-408c-463b-b81e-98650aef4314","added_by":"auto","created_at":"2025-11-18 15:40:29","extension":"jpeg","order_by":19,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":121512,"visible":true,"origin":"","legend":"","description":"","filename":"floatimage14.jpeg","url":"https://assets-eu.researchsquare.com/files/rs-7659075/v1/d750cae049119fc101cfbb4b.jpeg"},{"id":96197555,"identity":"f388cf4c-44cf-4b3b-9767-5ee50474fbcb","added_by":"auto","created_at":"2025-11-18 15:40:29","extension":"jpeg","order_by":20,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":168113,"visible":true,"origin":"","legend":"","description":"","filename":"floatimage15.jpeg","url":"https://assets-eu.researchsquare.com/files/rs-7659075/v1/d9f1769dcc1368ee49cd7963.jpeg"},{"id":96197547,"identity":"fe149d94-c20c-46ba-92cb-9b87edb6b407","added_by":"auto","created_at":"2025-11-18 15:40:29","extension":"jpeg","order_by":21,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":176918,"visible":true,"origin":"","legend":"","description":"","filename":"floatimage16.jpeg","url":"https://assets-eu.researchsquare.com/files/rs-7659075/v1/05d15d7a3e44ed40287ba253.jpeg"},{"id":96197535,"identity":"c3185c5a-c2dd-46e8-82ea-8ebe72fefe37","added_by":"auto","created_at":"2025-11-18 15:40:28","extension":"jpeg","order_by":22,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":189275,"visible":true,"origin":"","legend":"","description":"","filename":"floatimage17.jpeg","url":"https://assets-eu.researchsquare.com/files/rs-7659075/v1/dc4dad947538ef1e15e0c487.jpeg"},{"id":96252137,"identity":"a639a67d-bfc4-47f8-a7fc-9bd4ae630aad","added_by":"auto","created_at":"2025-11-19 07:40:29","extension":"jpeg","order_by":23,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":135711,"visible":true,"origin":"","legend":"","description":"","filename":"floatimage18.jpeg","url":"https://assets-eu.researchsquare.com/files/rs-7659075/v1/405cb11e89712e828c48722a.jpeg"},{"id":96251290,"identity":"d62d4fe5-1344-4013-8385-f76abdab87fe","added_by":"auto","created_at":"2025-11-19 07:39:36","extension":"jpeg","order_by":24,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":19433,"visible":true,"origin":"","legend":"","description":"","filename":"groupimage1.jpeg","url":"https://assets-eu.researchsquare.com/files/rs-7659075/v1/9cadfa0577201da08b2b3a43.jpeg"},{"id":96197550,"identity":"f7fb96ac-fecd-44a1-8edb-97166c2143d8","added_by":"auto","created_at":"2025-11-18 15:40:29","extension":"jpeg","order_by":25,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":19433,"visible":true,"origin":"","legend":"","description":"","filename":"groupimage1.jpeg","url":"https://assets-eu.researchsquare.com/files/rs-7659075/v1/9e80107dbed26a5a3031c052.jpeg"},{"id":96250114,"identity":"9e9efdb7-99c7-4f9c-ace2-c01f42910925","added_by":"auto","created_at":"2025-11-19 07:37:29","extension":"png","order_by":26,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":95532,"visible":true,"origin":"","legend":"","description":"","filename":"Onlinefloatimage1.png","url":"https://assets-eu.researchsquare.com/files/rs-7659075/v1/79d43e65d70819a28c644f0f.png"},{"id":96252605,"identity":"62b266eb-1178-4b47-a276-0c27f49bb2b3","added_by":"auto","created_at":"2025-11-19 07:41:16","extension":"png","order_by":27,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":95532,"visible":true,"origin":"","legend":"","description":"","filename":"Onlinefloatimage1.png","url":"https://assets-eu.researchsquare.com/files/rs-7659075/v1/5194d2a2bc2a2154ce230d7a.png"},{"id":96197537,"identity":"91b0847f-936a-4133-ac86-99be9b8cbff2","added_by":"auto","created_at":"2025-11-18 15:40:28","extension":"png","order_by":28,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":146608,"visible":true,"origin":"","legend":"","description":"","filename":"Onlinefloatimage11.png","url":"https://assets-eu.researchsquare.com/files/rs-7659075/v1/9f5e1f6b6f966f91628ace92.png"},{"id":96252128,"identity":"1718ade2-e28d-4e21-b574-79fa1320b0ac","added_by":"auto","created_at":"2025-11-19 07:40:29","extension":"png","order_by":29,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":935,"visible":true,"origin":"","legend":"","description":"","filename":"Onlinefloatimage12.png","url":"https://assets-eu.researchsquare.com/files/rs-7659075/v1/974f9d1aa673832ca1602a63.png"},{"id":96197551,"identity":"eb6ce410-9004-4159-891c-5c4742c83825","added_by":"auto","created_at":"2025-11-18 15:40:29","extension":"png","order_by":30,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":53008,"visible":true,"origin":"","legend":"","description":"","filename":"Onlinefloatimage13.png","url":"https://assets-eu.researchsquare.com/files/rs-7659075/v1/48904ee7210a8209dc510c4e.png"},{"id":96197546,"identity":"1f43e6f7-dc7e-4ca7-a89e-bd83644f3191","added_by":"auto","created_at":"2025-11-18 15:40:29","extension":"png","order_by":31,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":41810,"visible":true,"origin":"","legend":"","description":"","filename":"Onlinefloatimage14.png","url":"https://assets-eu.researchsquare.com/files/rs-7659075/v1/ca760dcdf5790f3e8c8945e2.png"},{"id":96197567,"identity":"6d11c8f9-ce0e-4253-ae31-2f3ed1d920ff","added_by":"auto","created_at":"2025-11-18 15:40:29","extension":"png","order_by":32,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":50096,"visible":true,"origin":"","legend":"","description":"","filename":"Onlinefloatimage15.png","url":"https://assets-eu.researchsquare.com/files/rs-7659075/v1/8080774277ed964cad18f297.png"},{"id":96197560,"identity":"04827fb9-c768-4934-84f8-3d12231466dc","added_by":"auto","created_at":"2025-11-18 15:40:29","extension":"png","order_by":33,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":56472,"visible":true,"origin":"","legend":"","description":"","filename":"Onlinefloatimage16.png","url":"https://assets-eu.researchsquare.com/files/rs-7659075/v1/f9ebc4b760c6beacd6984de6.png"},{"id":96197566,"identity":"f83105aa-54fc-481b-9521-4d56371c0b7c","added_by":"auto","created_at":"2025-11-18 15:40:29","extension":"png","order_by":34,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":63585,"visible":true,"origin":"","legend":"","description":"","filename":"Onlinefloatimage17.png","url":"https://assets-eu.researchsquare.com/files/rs-7659075/v1/08e2d52ad14a9bb58ce005fe.png"},{"id":96197552,"identity":"b677eada-34ac-4c13-886b-2ade869daed7","added_by":"auto","created_at":"2025-11-18 15:40:29","extension":"png","order_by":35,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":49781,"visible":true,"origin":"","legend":"","description":"","filename":"Onlinefloatimage18.png","url":"https://assets-eu.researchsquare.com/files/rs-7659075/v1/61e4486986511bdc4425b144.png"},{"id":96197553,"identity":"782a5a9c-5fee-4b69-a81d-cef2fa53218c","added_by":"auto","created_at":"2025-11-18 15:40:29","extension":"png","order_by":36,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":146608,"visible":true,"origin":"","legend":"","description":"","filename":"Onlinefloatimage2.png","url":"https://assets-eu.researchsquare.com/files/rs-7659075/v1/b383c14052f9f45dbefcc25f.png"},{"id":96252104,"identity":"d9cd9b5c-d3f4-4b2d-b2cb-6902571d2e16","added_by":"auto","created_at":"2025-11-19 07:40:27","extension":"png","order_by":37,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":935,"visible":true,"origin":"","legend":"","description":"","filename":"Onlinefloatimage3.png","url":"https://assets-eu.researchsquare.com/files/rs-7659075/v1/08ff1b75efae53e0ba3c445f.png"},{"id":96197563,"identity":"2daeb916-0d94-46b5-85b5-40f93c9cc5ed","added_by":"auto","created_at":"2025-11-18 15:40:29","extension":"png","order_by":38,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":53008,"visible":true,"origin":"","legend":"","description":"","filename":"Onlinefloatimage4.png","url":"https://assets-eu.researchsquare.com/files/rs-7659075/v1/c92bac097543aba2b1f35e1b.png"},{"id":96197541,"identity":"6bee7afa-d602-4ccd-807e-8ed505d7d1e6","added_by":"auto","created_at":"2025-11-18 15:40:28","extension":"png","order_by":39,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":41810,"visible":true,"origin":"","legend":"","description":"","filename":"Onlinefloatimage5.png","url":"https://assets-eu.researchsquare.com/files/rs-7659075/v1/2e0471e964a47b18733a0dbc.png"},{"id":96197540,"identity":"a02454b8-2200-4e3d-9c7a-f841c8094738","added_by":"auto","created_at":"2025-11-18 15:40:28","extension":"png","order_by":40,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":50096,"visible":true,"origin":"","legend":"","description":"","filename":"Onlinefloatimage6.png","url":"https://assets-eu.researchsquare.com/files/rs-7659075/v1/ff3a9f1b4b75cdf44b2ba29a.png"},{"id":96197558,"identity":"55dbad8e-bd03-408c-921e-1bf14a67d62e","added_by":"auto","created_at":"2025-11-18 15:40:29","extension":"png","order_by":41,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":56472,"visible":true,"origin":"","legend":"","description":"","filename":"Onlinefloatimage7.png","url":"https://assets-eu.researchsquare.com/files/rs-7659075/v1/9bb53328daca44c3c8647067.png"},{"id":96197531,"identity":"259737a9-bf71-4e93-8dbf-b136278a110f","added_by":"auto","created_at":"2025-11-18 15:40:28","extension":"png","order_by":42,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":63585,"visible":true,"origin":"","legend":"","description":"","filename":"Onlinefloatimage8.png","url":"https://assets-eu.researchsquare.com/files/rs-7659075/v1/a6b4f5c0dc38aeabbfe38fe6.png"},{"id":96197557,"identity":"bd375820-3521-4ddf-80fe-d3e7e8719e00","added_by":"auto","created_at":"2025-11-18 15:40:29","extension":"png","order_by":43,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":49781,"visible":true,"origin":"","legend":"","description":"","filename":"Onlinefloatimage9.png","url":"https://assets-eu.researchsquare.com/files/rs-7659075/v1/3d2c93873112b838ab377e23.png"},{"id":96252579,"identity":"eb84b2ab-1ac3-4933-8c23-d5f8f7f2a5f4","added_by":"auto","created_at":"2025-11-19 07:41:13","extension":"png","order_by":44,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":11285,"visible":true,"origin":"","legend":"","description":"","filename":"Onlinegroupimage1.png","url":"https://assets-eu.researchsquare.com/files/rs-7659075/v1/7c9e37d5761e1869e324cd32.png"},{"id":96197562,"identity":"641b4102-a385-4b5c-91e4-0518cd8a9e0e","added_by":"auto","created_at":"2025-11-18 15:40:29","extension":"png","order_by":45,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":11285,"visible":true,"origin":"","legend":"","description":"","filename":"Onlinegroupimage1.png","url":"https://assets-eu.researchsquare.com/files/rs-7659075/v1/1bcf0cae5f73653313f14c67.png"},{"id":96197564,"identity":"6aa4497a-936e-4755-8ae9-b07ce1181421","added_by":"auto","created_at":"2025-11-18 15:40:29","extension":"xml","order_by":46,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":245074,"visible":true,"origin":"","legend":"","description":"","filename":"d0acba5ab5924e46b32bc5abcabc6fbf1structuring.xml","url":"https://assets-eu.researchsquare.com/files/rs-7659075/v1/70a7c09767f8026c0b7db773.xml"},{"id":96250241,"identity":"103dc551-74ce-4539-b1df-83a664d97dd2","added_by":"auto","created_at":"2025-11-19 07:37:47","extension":"html","order_by":47,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":263409,"visible":true,"origin":"","legend":"","description":"","filename":"earlyproof.html","url":"https://assets-eu.researchsquare.com/files/rs-7659075/v1/ea2246ff3302c5140187080d.html"},{"id":97792325,"identity":"2fc5292b-be60-433f-aadd-c94256b61e20","added_by":"auto","created_at":"2025-12-09 12:08:51","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":952925,"visible":true,"origin":"","legend":"","description":"","filename":"Organicsubstratesforseedpotatoproduction.pdf","url":"https://assets-eu.researchsquare.com/files/rs-7659075/v1_covered_115705c7-b71b-4cec-a74d-7d7f2245225a.pdf"},{"id":96197530,"identity":"f97a270b-a781-4cea-a7fb-068e621e3d8d","added_by":"auto","created_at":"2025-11-18 15:40:28","extension":"xlsx","order_by":1,"title":"","display":"","copyAsset":false,"role":"supplement","size":110692,"visible":true,"origin":"","legend":"","description":"","filename":"Supplementarymaterial.xlsx","url":"https://assets-eu.researchsquare.com/files/rs-7659075/v1/a1e49003fd57c83751fdd2fb.xlsx"}],"financialInterests":"No competing interests reported.","formattedTitle":"Agronomic and physiological responses of seed-potato in different organic substrates","fulltext":[],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":true,"highlight":"","institution":"","isAcceptedByJournal":false,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":true,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true},"keywords":"IAC-Sprout/Seed Potato Technology, organic system, physiological responses, Solanum tuberosum L","lastPublishedDoi":"10.21203/rs.3.rs-7659075/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-7659075/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003ePotato cultivation is very important, especially in organic systems. However, high cost and low availability of seed-potatoes are limiting factors. This study aimed to formulate and select an organic substrate to promote vegetative and reproductive development for seed-potato production. Four experiments were carried out in a greenhouse, in a completely randomized design with five replicates, with the cultivars (cv.) Aracy, \u0026Aacute;gata and Aracy Ruiva, evaluating survival, leaf growth rate, gas exchange, agronomic variables, and macronutrients. Sprouts were used as propagation units. Experiment I was carried out to pre-select the best organic substrates, using cv. Aracy in 16 different substrates (S1, S2, S3, S4, S5, S6, S7, S8, S9, S10, S11, S12, S13, S14, S15, and S16), with survival rates ranging from 80 to 100%. Experiments II, III and IV were carried out with 8 substrates; the second experiment was conducted with the cv. Aracy, the third experiment was conducted with the cv. \u0026Aacute;gata and the forth experiment was conducted with the cv. Aracy Ruiva, and compared with commercial substrate (Mecplant\u0026reg;). The survival rates in the experiments were similar or higher than the commercial substrate. The substrates that promoted the highest leaf growth rate were S8 (experiments I and II), S11 (experiment III), and S9 (experiment IV). Substrates S8, S15, S1 and commercial promoted the highest instantaneous efficiency of carboxylation in plants of Aracy. The Aracy Ruiva grown in substrates S15, S8, S2 and commercial had higher internal concentration of CO\u003csub\u003e2\u003c/sub\u003e; and those grown in S3 had higher instantaneous efficiency of water use. The eight substrates selected provided similar mass, diameter and length of tubers produced by all cultivars, when compared to the commercial substrate. Significance was observed only for \u0026Aacute;gata, regarding the number of tubers. Among the organic substrates evaluated, substrate S11 is indicated for the production of seed-potatoes, as it promotes the highest survival among the evaluated substrates, high leaf area development, being the highest to the cv. \u0026Aacute;gata, promoted tuberization, increasing the tuber number produced by the plants and the tuber mass, and length and transversal diameter tuber of the evaluated cultivars and the substrate is formulated with washed coconut powder; sifted poultry litter; humus, limestone and sand, products easily to find by growers, with low cost and allowed to use in organic agriculture.\u003c/p\u003e","manuscriptTitle":"Agronomic and physiological responses of seed-potato in different organic substrates","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2025-11-18 15:40:22","doi":"10.21203/rs.3.rs-7659075/v1","editorialEvents":[{"type":"communityComments","content":0}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"39985136-aad1-40d9-87cf-f6212752b4f0","owner":[],"postedDate":"November 18th, 2025","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"posted","subjectAreas":[{"id":58121194,"name":"Biological sciences/Biotechnology"},{"id":58121197,"name":"Biological sciences/Plant sciences"}],"tags":[],"updatedAt":"2025-12-09T12:08:31+00:00","versionOfRecord":[],"versionCreatedAt":"2025-11-18 15:40:22","video":"","vorDoi":"","vorDoiUrl":"","workflowStages":[]},"version":"v1","identity":"rs-7659075","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-7659075","identity":"rs-7659075","version":["v1"]},"buildId":"8U1c8b4HqxoKbykW_rLl7","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}