Increase of CD69, CD161 and CD94 on NK cells in women with recurrent spontaneous abortion and in vitro fertilization failure

Mehri Ghafourian, Najmeh Karami, Ali Khodadadi, Roshan Nikbakht
Iranian journal of immunology : IJI · 2014 · vol. 11(2) , pp. 84–96 · doi:10.22034/iji.2014.16769 · PMID:24975965 · W35382065
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This study found increased expression of CD69, CD94, and CD161 on NK cells in women with recurrent spontaneous abortion and in vitro fertilization failure compared to controls.

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This case-control study investigated the expression of CD69, CD161, and CD94 markers on natural killer cells in women with recurrent spontaneous abortion or in vitro fertilization failure. Researchers analyzed peripheral blood samples from 23 patients with recurrent spontaneous abortion, 20 patients with in vitro fertilization failure, and 43 healthy control subjects using flow cytometry. The results demonstrated a significant increase in both the percentage of natural killer cells and the overall expression of these surface markers in both patient groups compared to their respective controls. The authors concluded that elevated CD69 and CD161 levels serve as potential immunological risk markers for these conditions, while noting uncertainty regarding the relevance of CD94 expression in endometrial natural killer cell activity. This paper does not explicitly discuss endometriosis or adenomyosis; it was included in the corpus via a keyword match in the upstream search index.

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Abstract

BACKGROUND: Recurrent spontaneous abortion (RSA) and in vitro fertilization (IVF) failure with unknown causes are the controversial issues that are probably related to the immune system. OBJECTIVE: To compare circulating NK cells expressing activation and inhibition surface markers between patients with RSA and IVF failure with those of healthy multiparous and successful IVF control women, respectively. METHODS: In this case-control study peripheral blood samples were collected from 43 patients who included 23 women with RSA and 20 with IVF failure, plus 43 healthy control women comprising of 36 normal multiparous women and seven women with successful IVF. The expression of CD69, CD94 and CD161 surface markers on CD56+NK cells were assessed using specific monoclonal antibodies by flowcytometry. RESULTS: The percentage of NK cells increased significantly in patients with RSA and in women with IVF failure in comparison to healthy multiparous and successful IVF control groups (p<0.001). The overall expression of CD69, CD94, CD161 were also increased significantly on NK cells in both patient groups compared to control groups (p<0.001). CONCLUSION: Elevated expression of CD69 and CD161 on NK cells can be considered as immunological risk markers in RSA and IVF failure. However, it is not clear if high expression of CD94 on peripheral blood NK cells is related to abnormal activity of endometrial NK cells.
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Abstract

Background: Recurrent spontaneous abortion (RSA) and in vitro fertilization (IVF) failure with unknown causes are the controversial issues that are probably related to the immune system.

Objective

To compare circulating NK cells expressing activation and inhibition surface markers between patients with RSA and IVF failure with those of healthy multiparous and successful IVF control women, respectively.

Methods

In this case-control study peripheral blood samples were collected from 43 patients who included 23 women with RSA and 20 with IVF failure, plus 43 healthy control women comprising of 36 normal multiparous women and seven women with successful IVF. The expression of CD69, CD94 and CD161 surface markers on CD56+NK cells were assessed using specific monoclonal antibodies by flowcytometry.

Results

The percentage of NK cells increased significantly in patients with RSA and in women with IVF failure in comparison to healthy multiparous and successful IVF control groups (p<0.001). The overall expression of CD69, CD94, CD161 were also increased significantly on NK cells in both patient groups compared to control groups (p<0.001).

Conclusion

Elevated expression of CD69 and CD161 on NK cells can be considered as immunological risk markers in RSA and IVF failure. However, it is not clear if high expression of CD94 on peripheral blood NK cells is related to abnormal activity of endometrial NK cells. Keywords

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MeSH descriptors

Abortion, Habitual Abortion, Habitual Antigens, CD Antigens, Differentiation, T-Lymphocyte Fertilization in Vitro Killer Cells, Natural Killer Cells, Natural Lectins, C-Type NK Cell Lectin-Like Receptor Subfamily B NK Cell Lectin-Like Receptor Subfamily D Abortion, Habitual Adult Antigens, CD Antigens, Differentiation, T-Lymphocyte Case-Control Studies CD69 Antigens Female Flow Cytometry Humans Immunophenotyping

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