Electrochemical Determination of Chloramphenicol in Milk and Eye-drop Using Easily Activated Screen Printed Carbon Electrodes

Abstract BackgroundAn analytical method that has fast response with cheap price and simple sample preparation is an ideal technique that the science need. An electrochemical sensor is an analytical method that suit for the above criteria to determine various samples using different electrode material. Providing detection method for chloramphenicol in farm product and drug sample using cheap electrode material is required due to its wide application in farm. ResultIn this study, direct and cost-effective activated SPCE was made electrochemically to determine chloramphenicol in eye-drop and pasteurized milk. The activation of the SPCE was straightforward and done by cycling 0.5 M KOH using LSV techniques. The employed surface imaging, spectroscopy, and electrochemical analysis show that there is an effective formation of new functional groups during the activation. The activated SPCE gave a superb current response for Chloramphenicol during the CV and SWV study using PBS of pH 6.5 compared to that of the bare one. Utilizing the optimal SWV conditions, a linear calibration curve was obtained using the range of 0.05‒100 µM with quite a small detection limit of 20 nM. ConclusionIn general, the higher sensitivity, stability and remarkable recovery results on the real sample analysis indicate that the activated screen printed carbon electrode can be applied to analyses sample in the field cause it have high stability, excellent reproducibility and long-term stability.