High platelet - lymphocyte ratio may predict poor therapeutic response in breast cancer patients treated with neoadjuvant chemotherapy

High platelet - lymphocyte ratio may predict poor therapeutic response in breast cancer patients treated with neoadjuvant chemotherapy | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Article High platelet - lymphocyte ratio may predict poor therapeutic response in breast cancer patients treated with neoadjuvant chemotherapy Wei Wang, Hao Chen, Ruoyan Zhao, Weijie Zhang, Yongzhong Yao This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-4692507/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract In this study, our aim was to explore the correlation between blood inflammatory markers (BIMs), including neutrophil to lymphocyte ratio (NLR), monocyte to lymphocyte ratio (MLR) and platelet to lymphocyte ratio (PLR), and the efficacy of neoadjuvant chemotherapy (NACT) in BC patients. Besides, the relationship between tumor infiltrating inflammatory cells (TIIs) and BIMs has also been preliminarily studied. A total of 315 BC patients between January 2018 and September 2022 were analyzed. Receiver operating characteristic curve was used to determine the cut-off value of each BIM. Logistic regression analysis were used to evaluated the predictive power of BIMs for pathological complete response (pCR). TIIs were assessed by immunohistochemistry, and their relationships with BIMs also were analyzed. The results showed that NLR, MLR, PLR in the pCR group were significantly lower than those in the non-pCR group after NACT (p ≤ 0.05). PLR had the largest area under the curve (AUC) (0.73) compared to NLR (0.57) and MLR (0.67) (p < 0.01). Univariate analysis showed ER, PR, HER2, NLR, MLR, PLR were significantly associated with pCR, but multivariate analysis found only HER2 and PLR were independently predictors for pCR (p < 0.01). PLR was positively correlated with the expression of P-selectin in tumor tissue (r: 0.26, p < 0.01). Survival analysis showed that NLR, MLR, PLR had no significant correlation with disease prognosis. In conclusion, PLR after NACT could serve as a predictor for pCR, and it correlated with the imbalance of the tumor-infiltrating platelet to lymphocyte ratio, which might cause drug resistance. Biological sciences/Cancer/Breast cancer Health sciences/Biomarkers/Predictive markers Biological sciences/Cancer/Cancer therapy/Chemotherapy Breast cancer Neoadjuvant chemotherapy Platelet to lymphocyte ratio Chemotherapy response Figures Figure 1 Introduction Neoadjuvant chemotherapy (NACT) has become the standard treatment for locally advanced breast cancer (BC). It not only can downstage locally advanced disease and make it operable, but also improve the breast-conserving surgery rate (1). However, the response to NACT among patients is variable. Although pathological complete response (pCR) is the most important curative indicator (2), it can only be evaluated on resected specimen. So far, there is no convenient biomarker that can reflect the tumor burden of patients during NACT in real time (3). Some studies have suggested that imaging, positron emission tomography, and gene expression profiling can predict the NACT outcome (4-6). But these methods are costly and not conducive to general clinical implementation. Inflammation has been considered one of the hallmarks of cancer (7). It can promote tumor initiation and progression, whereas escape from immune surveillance, which may favor cancer invasiveness (8). The main inflammation and immune cell components in the tumor microenvironment include tumor-infiltrating neutrophils (TINs), tumor-associated macrophages (TAMs), tumor-infiltrating lymphocytes (TILs) and tumor-educated platelets (TEPs) that are also considered to have certain proinflammatory activity (9-12). Some studies have found that TINs, TAMs and TEPs can promote angiogenesis and form an immunosuppressive microenvironment, which lead to tumor progression and drug resistance (13). While TILs usually inhibit tumor growth, and have a positive correlation with chemotherapy efficacy and prognosis of BC patients (14). Besides, one study has reported that intra-tumoral inflammatory cells to T cells ratio can reflect the inflammatory-immune imbalance in the tumor microenvironment, and has the potential to be a predictor of survival in some types of cancer (15). However, the analysis of inflammation and immune cell components in tumor microenvironment also is not convenient and timely. More importantly, even if TILs are considered to have a good application prospect, they are only limited to specific molecular subtypes of BC. As for TINs, TAMs and TEPs, they have not been applied in clinical practice at present due to the lack of support from large randomized controlled studies. Some researchers believe that since inflammation and immune cells in tumor microenvironment are derived from peripheral blood, then blood cell populations can provide information about the intra-tumoral status (16). There have been some studies reported that peripheral monocyte count was associated with the density of the TAMs, and high absolute monocyte count could predict poor survival in cancer patients. Besides, high platelet counts also were reported to be associated with adverse outcomes in BC (17). However, they are nonspecific inflammatory indexes and cannot be used as tumor biomarkers. In recent years, novel blood inflammatory markers (BIMs), such as neutrophil to lymphocyte ratio (NLR), platelet to lymphocyte ratio (PLR) and monocyte to lymphocyte ratio (MLR) derived from the count of inflammatory cells in peripheral blood, have been extensively studied. Several studies have found that some BIMs could be used as potential prognostic factors for survival in different types of cancers including BC (18-20). In the context of NACT, BIMs also have been suggested as potential predictors for pCR but with conflicting results (21, 22). Therefore, the significance of BIMs in BC remains unclear. In addition, whether inflammation cell components in the tumor microenvironment are affected by circulating cells, and whether local and circulating inflammation cells have consistent predictive effects on the efficacy of NACT is unclear. In this retrospective study, we intended to explore whether and which BIM could be used as a predictor for the efficacy of NACT. Besides, the corresponding cellular components in the tumor microenvironment also were evaluated to explore the relationship with circulating cells and their value in predicting the efficacy of NACT. Results Patients’ characteristics A total of 315 patients were included in this study, and the clinicopathological information of patients were shown in Table 1 . Invasive ductal carcinoma was diagnosed in 96.51% of the patients, and all patients were eligible for NACT according to the guidelines. Approximately 51.11% of patients received the docetaxel + anthracycline + cyclophosphamide (TAC/TEC × 6) regimen, about 36.19% of patients with HER2 expression received docetaxel + carboplatin + trastuzumab + pertuzumab (TCbHP × 6) or TCbH × 6 regimen, and about 6.98% received docetaxel + trastuzumab + pertuzumab (THP × 6) regimen. All patients received radical surgery at our hospital after chemotherapy. Surgical choices included breast-conserving surgery and mastectomy. If axillary lymph node biopsy confirmed metastasis before chemotherapy, axillary lymph node dissection was performed directly, otherwise sentinel lymph node biopsy was performed firstly. For patients accepted breast-conserving surgery or had positive axillary lymph nodes, postoperative radiotherapy was required. Pathological results of radical surgical specimens showed that 116 patients (36.83%) had no residual cancer cells or only ductal carcinoma in situ in the breast. Axillary lymph node metastasis was not found in 175 patients (55.56%). Besides, the Miller-Payne scoring system (MP) also was used to assess chemotherapy response of primary lesion, and the results showed that more than half (50.79%) of the patients achieved MP grade 4–5. At last, a total of 104 patients achieved pCR. Comparisons of BIMs before and after chemotherapy We first compared the differences in NLR, MLR, and PLR at baseline between the pCR and non-pCR groups, but the results showed no statistical difference. However, after chemotherapy, the average NLR, MLR and PLR in the pCR group were significantly lower than those in the non-pCR group (p < 0.05) (Table 2 ). This suggested that patients with lower BIMs after NACT seem to be more likely to reach pCR. In addition, ROC curve analysis was used to determine the optimal cut-off values for the NLR, MLR and PLR. The values of the area under the curve (AUC), sensitivity, and specificity in ROC analysis were shown in Table 3 . The cut-off values of the NLR, MLR and PLR after NACT were defined as 3.03, 0.29, 143.37 and 0.42 respectively and PLR had the largest AUC with 65% sensitivity and 73% specificity. Predictive factors for pCR in univariate and multivariate analyses Patients were divided into the" ≤ cut-off" group and the "> cut-off" group according to the cut-off value of each BIM. Univariate analysis showed that ER, PR, HER2 status before NACT and BIMs (including NLR, MLR, PLR) after chemotherapy were significantly correlated with pCR (p < 0.01). But multivariate logistic regression analysis suggested that only HER2 status (OR, 2.87; 95%CI, 1.54–5.37, p < 0.01) and PLR (OR, 0.34; 95%CI, 0.19–0.61, p < 0.01) were independent predictors for pCR (Table 4 ). In addition, subgroup analysis showed that patients in the PLR ≤ cut-off group had a higher pCR rate, which was not affected by the molecular subtype (Table 5 ). Immunohistochemical analysis Immunohistochemical staining was performed on the tissue sections of 125 patients, including 75 patients in the non-pCR group and 50 patients in the pCR group. The stained proteins included the lymphocyte marker CD3 and the activated platelet marker P-selectin. Protein expression levels were measured by Image J software. The results showed that there were few CD3 + cells in these tissue sections, but the P-selectin expression varied in different sections. Besides, the average expression level of P-selectin in the non-pCR group was significantly higher than that in the pCR group (0.34 ± 0.01 vs 0.29 ± 0.01, p < 0.01) (Fig. 1 ). Correlation analysis showed that the P-selectin expression was positively correlated with the PLR after NACT (r: 0.26, 95%CI: 0.08–0.41, p < 0.01), but had no obvious correlation with the platelet count in peripheral blood. Survival analysis The median follow-up time was 32 months (range 7–64 months). During the follow-up period, there were 6 deaths (1.90%), 20 lost to follow-up (6.35%), and 29 local recurrence or distant metastasis (9.20%) without death. The number of deaths, follow-up losses, and relapses was 2, 10, 11 cases, respectively in the PLR ≤ cut-off group, compared with 4, 10, 18 cases in the PLR > cut-off group. Survival analysis showed no significant difference in DFS and OS between the two groups. Besides, we also investigated the effect of NLR and MLR on DFS, but no meaningful results were obtained (Table 6 ). Discussion Blood routine test results, which reflect the individual’s system or local status, also have certain application value for cancer diagnosis ( 26 ). For example, neutrophils can produce cytokines, chemokines, and growth factors that promote angiogenesis, tumor cell proliferation and migration in the tumor microenvironment ( 27 ). Platelets, an important part of the blood clotting system, also can contribute to cancer-favored inflammation response ( 28 ), and high platelet counts were reported to be associated with adverse outcomes in BC ( 29 ). In contrast, lymphocytes in the blood are thought to can reflect the status of anti-tumor immunity ( 30 ). Based on the precious findings, NLR, MLR and PLR, which reflect the balance between inflammatory and immune response in cancer, have attracted more and more attention from researchers ( 31 ). However, with the increase of the number of studies, the value of these BIMs in BC especially in predicting the response to NACT, has become controversial. Furthermore, few studies have simultaneously compared the value of these BIMs in predicting response to NACT. In this study, we found that there was no difference in the BIMs before chemotherapy between the pCR group and the non-pCR group. However, the NLR, MLR and PLR in the pCR group all were significantly lower than those in the non-pCR group after NACT. This suggested that pCR might be easier to achieve in patients with weaker systemic inflammatory response after chemotherapy. ROC analysis showed that PLR, with 65% sensitivity and 73% specificity, had the largest AUC compared to NLR and MLR. Univariate analysis showed that parameters such as ER, PR, HER2, NLR, MLR, PLR were significantly associated with pCR. But multivariate logistic regression analysis showed that only HER2 and PLR were independently predictive factors of pCR. It is well known that HER2 was a positive predictor for BC patients with the application of anti-HER2 targeted drugs ( 32 ). Especially for patients receiving trastuzumab plus pertuzumab during NACT, the pCR rate even can reach more than 60% ( 33 ). Our results also support this conclusion. Although NLR and MLR after chemotherapy were associated with pCR rates in univariate analysis, they did not show statistical significance in multivariate analysis and survival analysis. The insignificance of their results might be attributed to the fact that the neutrophil and monocyte counts could be disturbed because of most patients were injected with granulocyte stimulating factor to prevent the neutropenia after chemotherapy. The incidence of thrombocytopenia during NACT was relatively low in this study, and patients were less likely to be treated for thrombocytopenia. Therefore, the platelet count could reflect the patient's actual condition. In addition to the role in hemostasis, platelets have been also thought to play an important role in promoting tumor progression and metastasis. First, platelets can produce a variety of cytokines such as platelet-derived growth factors and vascular endothelial growth factor A, to stimulate tumor progression and dissemination. Second, platelets can induce the epithelial–mesenchymal switch of tumor cells, reduce tumor cell anoikis in the blood. Third, platelets can protect tumor cells from being recognized by immune cells through forming a cell–fibrin–platelet aggregate surrounding tumor cells in the circulation. This is thought to play an important role in contributing to tumor metastasis ( 10 , 34 ). Therefore, the dynamics of PLR represents the disorder of inflammatory - immune status of patients. Prior to the present study, there have been some studies that used PLR as a predictive marker for NACT in BC. Some reported that increased PLR during chemotherapy predicted higher pCR rate, while others had the opposite conclusions ( 35 , 36 ). A meta-analysis has demonstrated that increased PLR during NACT could be as a prognostic biomarker for poor overall survival and DFS ( 37 ). Our results supported that increased PLR suggested poor efficacy of NACT. Some researchers believed that cell component in blood might can provide information about the status of tumor microenvironment, since most inflammation and immune cells in tumor microenvironment were derived from peripheral blood ( 16 ). However, there are few studies to investigate the relationship between peripheral blood cells and corresponding infiltrating cells in tumor stroma. One important reason is the lack of a specific marker to label the same type of cells in both blood and stroma. P-selectin, also known as CD62P, is stored in α-granules and Weibel–Palade bodies of platelets and endothelial cells, respectively. P-selectin expression is considered to be a marker of platelet activation. P-selectin on activated platelets can mediate interactions with monocytes, instigating paracrine-signaling mechanisms that lead to enhanced inflammation, as well as inducing factors that trigger thrombosis ( 38 ). In this study, P-selectin staining was performed on the specimens of 125 patients. The results showed that the expression of P-selectin in tumor microenvironment was significantly positively correlated with PLR, suggesting that the expression of P-selectin in stroma might be affected by platelets in peripheral blood, but the mechanism needs to be further studied. There are still some limitations in this study. First, this is a single-center retrospective study with a small sample size and the follow-up time is short, which makes some conclusions less convincing. Second, although P-selectin is thought to be expressed mainly on activated platelets, it is not a platelet-specific marker. The expression levels of P-selectin in tissues were not exactly equivalent to TEPs. Therefore, the significance of correlation analysis between P-selectin and PLR remains to be further explored. Conclusion This study confirmed that systemic inflammatory-immune imbalance after NACT was associated with poor chemotherapy response, and PLR after chemotherapy could serve as a potential predictor. In addition, we found that the expression of P-selectin in tumor microenvironment might be affected by PLR in peripheral blood, but the exact mechanism needs to be further studied. Methods Patients This is a single-center retrospective study. The subjects included clinically diagnosed BC patients who received NACT followed by surgery at our hospital between January 2018 and September 2022. Inclusion criteria included: ( 1 ) age > 18 years old; ( 2 ) invasive BC confirmed by biopsy; ( 3 ) at least immunohistochemical results for ER, PR, HER2, and Ki67 were available; ( 4 ) all patients should meet the NACT criteria according to established guidelines ( 23 ); ( 5 ) radical surgery for each patient performed at Nanjing Drum Tower Hospital after NACT. Exclusion criteria included: ( 1 ) patients with clinical stage IV or inflammatory breast cancer or history of previous malignant tumor; ( 2 ) clinicopathological data were insufficient; ( 3 ) patients who did not meet the NACT criteria or terminated chemotherapy prematurely; ( 4 ) patients who did not undergo radical surgery at Nanjing Drum Tower Hospital after NACT. Ethical consideration This study was approved by the ethical and scientific committee of Nanjing Drum Tower Hospital (number 2023-460-02) and followed the principles of Declaration of Helsinki. Data collection and processing Medical electronic records and pathology reports of patients were reviewed. The recorded information included: age, body mass index (BMI), menstrual status, clinical stage before chemotherapy (tumor size, axillary lymph node status, distant metastasis or not), pathological results of biopsy and radical surgical specimens, NACT regimens, imaging assessment results during NACT, and surgery methods. Hormone receptor (HR), HER2, and Ki67 index were characterized on biopsy and radical surgical specimens in the central laboratory of our hospital. HER2 was determined according to ASCO/CAP guidelines ( 24 ). HR + was defined if more than 1% of tumor cells were positive for ER/PR immunohistochemical staining. The absolute counts of neutrophils, monocytes, platelets and lymphocytes in peripheral blood of patients before the first NACT and after the last chemotherapy were recorded in an excel sheet. And then they were used to calculate NLR, MLR, and PLR. Immunohistochemistry Paraffin sections were deparaffinized at 65℃ for 1 hour and treated in a pressure boiler with EDTA for 10 min. The sections were placed in 3% hydrogen peroxide solution and incubated at room temperature for 10 min to block endogenous peroxidase. Each section was washed 3 times with phosphate-buffered saline (PBS), sealed with Protein Block Serum-free for 20 min and then incubated with primary rabbit monoclonal antibody (Abcam, Cambridge, USA) overnight at 4ºC. After that, the sections were incubated with secondary antibody (Dako, California, USA) for 30 min, washed with PBS for 3 times. Tissues were stained for 5 min with fresh DAB solution and then counterstained with Mayer's hematoxylin and mounted in Permount (Fisher Scientific, New Jersey, USA). Samples were evaluated by one independent observer using an optical microscope. Only cancer cells with a distinct brown staining of the nucleus were considered positive. Outcomes The primary outcome measured in this study was pCR. The pCR was defined by ypT0/Tis, N0, M0. Secondary outcome were disease-free survival (DFS) and overall survival (OS). DFS was defined as the time from radical surgery after NACT until any distant or local recurrence and OS was defined as the period from the date of diagnosis until the date of death assessed by Response Evaluation Criteria in Solid Tumors 1.1 (RECIST 1.1) ( 25 ). Statistical analysis The statistical analysis was done using SPSS 20 (IBM Corp., Armonk, NY). Continuous variables were expressed as "mean ± standard error", and categorical variables were expressed as percentage (%). Chi-square test or Fisher's exact test was used to compare the categorical parameters between the two groups. T-test were used to compare the normally distributed continuous variables. Correlation analysis of categorical parameters was done by Pearson χ² test. The optimal cut-off value of each index was determined using receiver operating characteristic (ROC) curve analysis. Kaplan-Meier method was used to plot survival curve and log-rank test was used to compare survival difference. A p value < 0.05 was considered statistically significant. Declarations Competing interests The authors declare no competing interests. Funding This work was supported by the Natural Science Foundation of Jiangsu Province [grant number BK20190126]. Author Contribution W.W. designed the study, collected and analyzed the data, prepared tables and figures and drafted the first manuscript. H.C. and R.Z. collected the data. W.Z. provided critical comments for this paper. Y.Y. reviewed the final draft. All authors contributed to the article and approved the submitted version. Data Availability The dataset utilized and analyzed in the current research is accessible from the corresponding authors upon reasonable request. 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BMI: body mass index, TAC: docetaxel + Adriamycin + cyclophosphamide, TCbHP: docetaxel + carboplatin + trastuzumab + pertuzumab, TCbH: docetaxel + carboplatin + trastuzumab, THP: docetaxel + trastuzumab + pertuzumab, pCR: pathological complete response, MP: Miller-Payne grading system. N (315) Percentage (%) Age ≤ 35 40 12.7 36–55 183 58.1 ≥ 56 92 29.2 BMI < 24 165 52.38 ≥ 24 150 47.62 Menopausal status pre-menopausal 170 53.97 post-menopausal 145 46.03 T stage T1 54 17.14 T2 218 69.21 T3-T4 43 13.65 Axillary lymph node negative 41 13.02 positive 224 71.11 uncertain 50 15.87 Histological type invasive ductal carcinoma 304 96.51 others 11 3.49 Histopathological grade I-II 213 67.62 III 102 32.38 ER positive 185 58.73 negative 130 41.27 PR positive 152 48.25 negative 163 51.75 HER2 positive 147 46.67 negative 168 53.33 Ki67 ≤ 14% 11 3.49 > 14% 304 96.51 Chemotherapy regimens TAC × 6 161 51.11 TCbH/TCbHP × 6 114 36.19 THP × 6 22 6.98 others 18 5.72 pCR yes 104 33.02 no 211 66.98 pT T0/Tis 116 36.83 T ≥ 1 199 63.17 pN N0 175 55.56 N+ 140 44.44 MP 4–5 160 50.79 1–3 155 49.21 Table 2 Comparisons of BIMs between non-pCR and pCR group. NLR: neutrophil to lymphocyte ratio, MLR: monocyte-lymphocyte ratio, PLR: platelet to lymphocyte ratio, NACT: neoadjuvant chemotherapy. Pre-NACT Post-NACT non-pCR pCR p non-pCR pCR p NLR 2.22 ± 0.07 2.15 ± 0.10 0.56 3.11 ± 0.17 2.58 ± 0.19 0.05 MLR 0.21 ± 0.01 0.19 ± 0.01 0.15 0.43 ± 0.02 0.32 ± 0.03 < 0.01 PLR 145 ± 4.25 138.7 ± 4.56 0.36 211.20 ± 10.06 148.80 ± 9.89 < 0.01 Table 3 ROC curve analyses of BIMs for pCR. ROC: receiver operating characteristic, AUC: area under the curve, CI: confidence interval. Cut-off value AUC 95% CI p Sensitivity Specificity NLR 3.03 0.57 0.51–0.64 0.04 0.76 0.40 MLR 0.29 0.67 0.60–0.73 < 0.01 0.61 0.65 PLR 143.37 0.73 0.67–0.79 < 0.01 0.65 0.73 Table 4 Univariate and multivariate logistic regression analysis for the predictors of pCR. OR: odds ratio Univariate analysis Multivariate analysis OR 95%CI p OR 95%CI p Lower Upper Lower Upper Age (≤ 45 vs > 45) 1.62 0.96 2.64 0.07 Menopausal status (pre vs post) 1.51 0.94 2.42 0.09 BMI (< 24 vs ≥ 24) 1.03 0.64 1.65 0.91 T (T1-2 vs T3-4) 0.57 0.27 1.22 0.14 Node (negative vs positive) 0.84 0.42 1.68 0.62 WHO (I-II vs III) 1.41 0.86 2.31 0.17 ER (negative vs positive) 0.41 0.25 0.66 < 0.01 0.63 0.32 1.24 0.18 PR (negative vs positive) 0.34 0.21 0.55 < 0.01 0.51 0.26 1.02 0.06 HER2 (negative vs positive) 5.35 3.19 8.99 < 0.01 2.87 1.54 5.37 14%) 1.33 0.35 5.11 0.68 NLR (≤ cut-off vs > cut-off) 0.48 0.28 0.81 cut-off) 0.34 0.21 0.56 cut-off) 0.2 0.12 0.33 < 0.01 0.34 0.19 0.61 cut-off OR 95%CI p Luminal A/B 0.46 0.24–0.86 0.02 non-pCR 45 99 pCR 27 27 HER2+ (ER-, PR-) 0.32 0.11–0.94 0.04 non-pCR 13 14 pCR 26 9 TNBC 0.24 0.07–0.89 0.04 non-pCR 7 33 pCR 7 8 TNBC: triple negative breast cancer Table 6 Survival curves obtained with Kaplan-Meier analysis. DFS: disease-free survival, OS: overall survival DFS(≤ cut-off vs > cut-off) OS(≤ cut-off vs > cut-off) ratio 95% CI p ratio 95% CI p NLR 1.06 0.50–2.27 0.88 1.22 0.23–6.32 0.82 MLR 1.40 0.66–2.94 0.38 0.98 0.18–5.33 0.98 PLR 0.95 0.45–2.02 0.89 1.12 0.19–6.43 0.90 Additional Declarations No competing interests reported. Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-4692507","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Article","associatedPublications":[],"authors":[{"id":333761304,"identity":"45606dbc-e6c5-43c7-a07d-07933698d5f7","order_by":0,"name":"Wei Wang","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAAA0UlEQVRIiWNgGAWjYFCCAzAGY+ODhIoa0rQ0Gzw4c4w0+9gkH7YwE1Zmzng6TZqn5o7dhuPNbRWJDWwM/O3dCXi1WDac3SbNc+xZ8oYzB9tuJO6QYZA4c3YDXi0GB4BactgOJ5vdSARqOcPGYCCRS4yWf0At9x+2FSS2MROpJbftsJ3ZDcY2BmK1bLb+23c4wf5MYrNEwpljPIT9cuPsxpszvh22l2w//vDjj4oaOf72XvxaGCQOgKnEBiifB79yEOCHqLUnrHIUjIJRMApGLAAApsBUrgPA5GYAAAAASUVORK5CYII=","orcid":"","institution":"Nanjing Drum Tower Hospital, the Affiliated Hospital of Nanjing University Medical School","correspondingAuthor":true,"prefix":"","firstName":"Wei","middleName":"","lastName":"Wang","suffix":""},{"id":333761305,"identity":"74ddd0f7-0463-4d81-aba7-c63b498261db","order_by":1,"name":"Hao Chen","email":"","orcid":"","institution":"Nanjing Drum Tower Hospital, the Affiliated Hospital of Nanjing University Medical School","correspondingAuthor":false,"prefix":"","firstName":"Hao","middleName":"","lastName":"Chen","suffix":""},{"id":333761306,"identity":"22730b22-06c7-46c6-bb56-e4fc09e427cb","order_by":2,"name":"Ruoyan Zhao","email":"","orcid":"","institution":"Nanjing Drum Tower Hospital, the Affiliated Hospital of Nanjing University Medical School","correspondingAuthor":false,"prefix":"","firstName":"Ruoyan","middleName":"","lastName":"Zhao","suffix":""},{"id":333761307,"identity":"f2683782-9621-4d0b-83ab-f08d0c00ab37","order_by":3,"name":"Weijie Zhang","email":"","orcid":"","institution":"Nanjing Drum Tower Hospital, the Affiliated Hospital of Nanjing University Medical School","correspondingAuthor":false,"prefix":"","firstName":"Weijie","middleName":"","lastName":"Zhang","suffix":""},{"id":333761308,"identity":"627c190d-cf87-43e9-9b9e-3f19a73c3f44","order_by":4,"name":"Yongzhong Yao","email":"","orcid":"","institution":"Nanjing Drum Tower Hospital, the Affiliated Hospital of Nanjing University Medical School","correspondingAuthor":false,"prefix":"","firstName":"Yongzhong","middleName":"","lastName":"Yao","suffix":""}],"badges":[],"createdAt":"2024-07-05 13:15:57","currentVersionCode":1,"declarations":{"humanSubjects":false,"vertebrateSubjects":false,"conflictsOfInterestStatement":false,"humanSubjectEthicalGuidelines":false,"humanSubjectConsent":false,"humanSubjectClinicalTrial":false,"humanSubjectCaseReport":false,"vertebrateSubjectEthicalGuidelines":false},"doi":"10.21203/rs.3.rs-4692507/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-4692507/v1","draftVersion":[],"editorialEvents":[],"editorialNote":"","failedWorkflow":false,"files":[{"id":62152523,"identity":"78dc273a-58fd-4924-a33f-c636c9d37eae","added_by":"auto","created_at":"2024-08-09 20:51:15","extension":"jpeg","order_by":1,"title":"Figure 1","display":"","copyAsset":false,"role":"figure","size":78278,"visible":true,"origin":"","legend":"\u003cp\u003eImmunohistochemical staining results of P-selectin and its correlation with PLR. A: Representative immunohistochemical results of P-selectin in the non-pCR group. B: Representative immunohistochemical results of P-selectin in the pCR group. C: Quantitative comparison of P-selectin between the two groups. D: Correlation analysis between P-selectin and PLR after NACT.\u003c/p\u003e","description":"","filename":"floatimage1.jpeg","url":"https://assets-eu.researchsquare.com/files/rs-4692507/v1/672eeade225a98c92924fa6c.jpeg"},{"id":64426786,"identity":"4fd55573-5c38-423d-bc81-3c9c43a19bde","added_by":"auto","created_at":"2024-09-13 04:25:36","extension":"pdf","order_by":0,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":774618,"visible":true,"origin":"","legend":"","description":"","filename":"manuscript.pdf","url":"https://assets-eu.researchsquare.com/files/rs-4692507/v1/5034de08-9250-48b8-813d-f7001943b29c.pdf"}],"financialInterests":"No competing interests reported.","formattedTitle":"High platelet - lymphocyte ratio may predict poor therapeutic response in breast cancer patients treated with neoadjuvant chemotherapy ","fulltext":[{"header":"Introduction","content":"\u003cp\u003eNeoadjuvant chemotherapy (NACT) has become the standard treatment for locally advanced breast cancer (BC). It not only can downstage locally advanced disease and make it operable, but also improve the breast-conserving surgery rate (1). However, the response to NACT among patients is variable. Although pathological complete response (pCR) is the most important curative indicator (2), it can only be evaluated on resected specimen. So far, there is no convenient biomarker that can reflect the tumor burden of patients during NACT in real time (3). Some studies have suggested that imaging, positron emission tomography, and gene expression profiling can predict the NACT outcome (4-6). But these methods are costly and not conducive to general clinical implementation.\u003c/p\u003e\n\u003cp\u003eInflammation has been considered one of the hallmarks of cancer (7). It can promote tumor initiation and progression, whereas escape from immune surveillance, which may favor cancer invasiveness (8). The main inflammation and immune cell components in the tumor microenvironment include tumor-infiltrating neutrophils (TINs), tumor-associated macrophages (TAMs), tumor-infiltrating lymphocytes (TILs) and tumor-educated platelets (TEPs) that are also considered to have certain proinflammatory activity (9-12). Some studies have found that TINs, TAMs and TEPs can promote angiogenesis and form an immunosuppressive microenvironment, which lead to tumor progression and drug resistance (13). While TILs usually inhibit tumor growth, and have a positive correlation with chemotherapy efficacy and prognosis of BC patients (14). Besides, one study has reported that intra-tumoral inflammatory cells to T cells ratio can reflect the inflammatory-immune imbalance in the tumor microenvironment, and has the potential to be a predictor of survival in some types of cancer\u0026nbsp;(15). However, the analysis of inflammation and immune cell components in tumor microenvironment also is not convenient and timely. More importantly, even if TILs are considered to have a good application prospect, they are only limited to specific molecular subtypes of BC. As for TINs, TAMs and TEPs, they have not been applied in clinical practice at present due to the lack of support from large randomized controlled studies.\u0026nbsp;\u003c/p\u003e\n\u003cp\u003eSome researchers believe that since inflammation and immune cells in tumor microenvironment are derived from peripheral blood, then blood cell populations can provide information about the intra-tumoral status (16). There have been some studies reported that peripheral monocyte count was associated with the density of the TAMs, and high absolute monocyte count could predict poor survival in cancer patients. Besides, high platelet counts also were reported to be associated with adverse outcomes in BC (17). However, they are nonspecific inflammatory indexes and cannot be used as tumor biomarkers. In recent years, novel blood inflammatory markers (BIMs), such as neutrophil to lymphocyte ratio (NLR), platelet to lymphocyte ratio (PLR) and monocyte to lymphocyte ratio (MLR) derived from the count of inflammatory cells in peripheral blood, have been extensively studied. Several studies have found that some BIMs could be used as potential prognostic factors for survival in different types of cancers including BC (18-20). In the context of NACT, BIMs also have been suggested as potential predictors for pCR but with conflicting results (21, 22). Therefore, the significance of BIMs in BC remains unclear. In addition, whether inflammation cell components in the tumor microenvironment are affected by circulating cells, and whether local and circulating inflammation cells have consistent predictive effects on the efficacy of NACT is unclear.\u003c/p\u003e\n\u003cp\u003eIn this retrospective study, we intended to explore whether and which BIM could be used as a predictor for the efficacy of NACT. Besides, the corresponding cellular components in the tumor microenvironment also were evaluated to explore the relationship with circulating cells and their value in predicting the efficacy of NACT.\u003c/p\u003e"},{"header":"Results","content":"\u003cdiv id=\"Sec2\" class=\"Section2\"\u003e \u003ch2\u003ePatients\u0026rsquo; characteristics\u003c/h2\u003e \u003cp\u003eA total of 315 patients were included in this study, and the clinicopathological information of patients were shown in Table \u003cspan refid=\"Tab1\" class=\"InternalRef\"\u003e1\u003c/span\u003e. Invasive ductal carcinoma was diagnosed in 96.51% of the patients, and all patients were eligible for NACT according to the guidelines. Approximately 51.11% of patients received the docetaxel\u0026thinsp;+\u0026thinsp;anthracycline\u0026thinsp;+\u0026thinsp;cyclophosphamide (TAC/TEC \u0026times; 6) regimen, about 36.19% of patients with HER2 expression received docetaxel\u0026thinsp;+\u0026thinsp;carboplatin\u0026thinsp;+\u0026thinsp;trastuzumab\u0026thinsp;+\u0026thinsp;pertuzumab (TCbHP \u0026times; 6) or TCbH \u0026times; 6 regimen, and about 6.98% received docetaxel\u0026thinsp;+\u0026thinsp;trastuzumab\u0026thinsp;+\u0026thinsp;pertuzumab (THP \u0026times; 6) regimen. All patients received radical surgery at our hospital after chemotherapy. Surgical choices included breast-conserving surgery and mastectomy. If axillary lymph node biopsy confirmed metastasis before chemotherapy, axillary lymph node dissection was performed directly, otherwise sentinel lymph node biopsy was performed firstly. For patients accepted breast-conserving surgery or had positive axillary lymph nodes, postoperative radiotherapy was required.\u003c/p\u003e \u003cp\u003ePathological results of radical surgical specimens showed that 116 patients (36.83%) had no residual cancer cells or only ductal carcinoma in situ in the breast. Axillary lymph node metastasis was not found in 175 patients (55.56%). Besides, the Miller-Payne scoring system (MP) also was used to assess chemotherapy response of primary lesion, and the results showed that more than half (50.79%) of the patients achieved MP grade 4\u0026ndash;5. At last, a total of 104 patients achieved pCR.\u003c/p\u003e \u003c/div\u003e\n\u003ch3\u003eComparisons of BIMs before and after chemotherapy\u003c/h3\u003e\n\u003cp\u003eWe first compared the differences in NLR, MLR, and PLR at baseline between the pCR and non-pCR groups, but the results showed no statistical difference. However, after chemotherapy, the average NLR, MLR and PLR in the pCR group were significantly lower than those in the non-pCR group (p\u0026thinsp;\u0026lt;\u0026thinsp;0.05) (Table\u0026nbsp;\u003cspan refid=\"Tab2\" class=\"InternalRef\"\u003e2\u003c/span\u003e). This suggested that patients with lower BIMs after NACT seem to be more likely to reach pCR. In addition, ROC curve analysis was used to determine the optimal cut-off values for the NLR, MLR and PLR. The values of the area under the curve (AUC), sensitivity, and specificity in ROC analysis were shown in Table \u003cspan refid=\"Tab3\" class=\"InternalRef\"\u003e3\u003c/span\u003e. The cut-off values of the NLR, MLR and PLR after NACT were defined as 3.03, 0.29, 143.37 and 0.42 respectively and PLR had the largest AUC with 65% sensitivity and 73% specificity.\u003c/p\u003e \u003cdiv id=\"Sec4\" class=\"Section2\"\u003e \u003ch2\u003ePredictive factors for pCR in univariate and multivariate analyses\u003c/h2\u003e \u003cp\u003ePatients were divided into the\" \u0026le; cut-off\" group and the \"\u0026gt; cut-off\" group according to the cut-off value of each BIM. Univariate analysis showed that ER, PR, HER2 status before NACT and BIMs (including NLR, MLR, PLR) after chemotherapy were significantly correlated with pCR (p\u0026thinsp;\u0026lt;\u0026thinsp;0.01). But multivariate logistic regression analysis suggested that only HER2 status (OR, 2.87; 95%CI, 1.54\u0026ndash;5.37, p\u0026thinsp;\u0026lt;\u0026thinsp;0.01) and PLR (OR, 0.34; 95%CI, 0.19\u0026ndash;0.61, p\u0026thinsp;\u0026lt;\u0026thinsp;0.01) were independent predictors for pCR (Table\u0026nbsp;\u003cspan refid=\"Tab4\" class=\"InternalRef\"\u003e4\u003c/span\u003e). In addition, subgroup analysis showed that patients in the PLR\u0026thinsp;\u0026le;\u0026thinsp;cut-off group had a higher pCR rate, which was not affected by the molecular subtype (Table\u0026nbsp;\u003cspan refid=\"Tab5\" class=\"InternalRef\"\u003e5\u003c/span\u003e).\u003c/p\u003e \u003c/div\u003e \u003cdiv id=\"Sec5\" class=\"Section2\"\u003e \u003ch2\u003eImmunohistochemical analysis\u003c/h2\u003e \u003cp\u003eImmunohistochemical staining was performed on the tissue sections of 125 patients, including 75 patients in the non-pCR group and 50 patients in the pCR group. The stained proteins included the lymphocyte marker CD3 and the activated platelet marker P-selectin. Protein expression levels were measured by Image J software. The results showed that there were few CD3\u0026thinsp;+\u0026thinsp;cells in these tissue sections, but the P-selectin expression varied in different sections. Besides, the average expression level of P-selectin in the non-pCR group was significantly higher than that in the pCR group (0.34\u0026thinsp;\u0026plusmn;\u0026thinsp;0.01 \u003cem\u003evs\u003c/em\u003e 0.29\u0026thinsp;\u0026plusmn;\u0026thinsp;0.01, p\u0026thinsp;\u0026lt;\u0026thinsp;0.01) (Fig.\u0026nbsp;\u003cspan refid=\"Fig1\" class=\"InternalRef\"\u003e1\u003c/span\u003e). Correlation analysis showed that the P-selectin expression was positively correlated with the PLR after NACT (r: 0.26, 95%CI: 0.08\u0026ndash;0.41, p\u0026thinsp;\u0026lt;\u0026thinsp;0.01), but had no obvious correlation with the platelet count in peripheral blood.\u003c/p\u003e \u003c/div\u003e \u003cdiv id=\"Sec6\" class=\"Section2\"\u003e \u003ch2\u003eSurvival analysis\u003c/h2\u003e \u003cp\u003eThe median follow-up time was 32 months (range 7\u0026ndash;64 months). During the follow-up period, there were 6 deaths (1.90%), 20 lost to follow-up (6.35%), and 29 local recurrence or distant metastasis (9.20%) without death. The number of deaths, follow-up losses, and relapses was 2, 10, 11 cases, respectively in the PLR\u0026thinsp;\u0026le;\u0026thinsp;cut-off group, compared with 4, 10, 18 cases in the PLR\u0026thinsp;\u0026gt;\u0026thinsp;cut-off group. Survival analysis showed no significant difference in DFS and OS between the two groups. Besides, we also investigated the effect of NLR and MLR on DFS, but no meaningful results were obtained (Table\u0026nbsp;\u003cspan refid=\"Tab6\" class=\"InternalRef\"\u003e6\u003c/span\u003e).\u003c/p\u003e \u003c/div\u003e"},{"header":"Discussion","content":"\u003cp\u003eBlood routine test results, which reflect the individual\u0026rsquo;s system or local status, also have certain application value for cancer diagnosis (\u003cspan citationid=\"CR26\" class=\"CitationRef\"\u003e26\u003c/span\u003e). For example, neutrophils can produce cytokines, chemokines, and growth factors that promote angiogenesis, tumor cell proliferation and migration in the tumor microenvironment (\u003cspan citationid=\"CR27\" class=\"CitationRef\"\u003e27\u003c/span\u003e). Platelets, an important part of the blood clotting system, also can contribute to cancer-favored inflammation response (\u003cspan citationid=\"CR28\" class=\"CitationRef\"\u003e28\u003c/span\u003e), and high platelet counts were reported to be associated with adverse outcomes in BC (\u003cspan citationid=\"CR29\" class=\"CitationRef\"\u003e29\u003c/span\u003e). In contrast, lymphocytes in the blood are thought to can reflect the status of anti-tumor immunity (\u003cspan citationid=\"CR30\" class=\"CitationRef\"\u003e30\u003c/span\u003e). Based on the precious findings, NLR, MLR and PLR, which reflect the balance between inflammatory and immune response in cancer, have attracted more and more attention from researchers (\u003cspan citationid=\"CR31\" class=\"CitationRef\"\u003e31\u003c/span\u003e). However, with the increase of the number of studies, the value of these BIMs in BC especially in predicting the response to NACT, has become controversial. Furthermore, few studies have simultaneously compared the value of these BIMs in predicting response to NACT.\u003c/p\u003e \u003cp\u003eIn this study, we found that there was no difference in the BIMs before chemotherapy between the pCR group and the non-pCR group. However, the NLR, MLR and PLR in the pCR group all were significantly lower than those in the non-pCR group after NACT. This suggested that pCR might be easier to achieve in patients with weaker systemic inflammatory response after chemotherapy. ROC analysis showed that PLR, with 65% sensitivity and 73% specificity, had the largest AUC compared to NLR and MLR. Univariate analysis showed that parameters such as ER, PR, HER2, NLR, MLR, PLR were significantly associated with pCR. But multivariate logistic regression analysis showed that only HER2 and PLR were independently predictive factors of pCR.\u003c/p\u003e \u003cp\u003eIt is well known that HER2 was a positive predictor for BC patients with the application of anti-HER2 targeted drugs (\u003cspan citationid=\"CR32\" class=\"CitationRef\"\u003e32\u003c/span\u003e). Especially for patients receiving trastuzumab plus pertuzumab during NACT, the pCR rate even can reach more than 60% (\u003cspan citationid=\"CR33\" class=\"CitationRef\"\u003e33\u003c/span\u003e). Our results also support this conclusion. Although NLR and MLR after chemotherapy were associated with pCR rates in univariate analysis, they did not show statistical significance in multivariate analysis and survival analysis. The insignificance of their results might be attributed to the fact that the neutrophil and monocyte counts could be disturbed because of most patients were injected with granulocyte stimulating factor to prevent the neutropenia after chemotherapy. The incidence of thrombocytopenia during NACT was relatively low in this study, and patients were less likely to be treated for thrombocytopenia. Therefore, the platelet count could reflect the patient's actual condition.\u003c/p\u003e \u003cp\u003eIn addition to the role in hemostasis, platelets have been also thought to play an important role in promoting tumor progression and metastasis. First, platelets can produce a variety of cytokines such as platelet-derived growth factors and vascular endothelial growth factor A, to stimulate tumor progression and dissemination. Second, platelets can induce the epithelial\u0026ndash;mesenchymal switch of tumor cells, reduce tumor cell anoikis in the blood. Third, platelets can protect tumor cells from being recognized by immune cells through forming a cell\u0026ndash;fibrin\u0026ndash;platelet aggregate surrounding tumor cells in the circulation. This is thought to play an important role in contributing to tumor metastasis (\u003cspan citationid=\"CR10\" class=\"CitationRef\"\u003e10\u003c/span\u003e, \u003cspan citationid=\"CR34\" class=\"CitationRef\"\u003e34\u003c/span\u003e). Therefore, the dynamics of PLR represents the disorder of inflammatory - immune status of patients. Prior to the present study, there have been some studies that used PLR as a predictive marker for NACT in BC. Some reported that increased PLR during chemotherapy predicted higher pCR rate, while others had the opposite conclusions (\u003cspan citationid=\"CR35\" class=\"CitationRef\"\u003e35\u003c/span\u003e, \u003cspan citationid=\"CR36\" class=\"CitationRef\"\u003e36\u003c/span\u003e). A meta-analysis has demonstrated that increased PLR during NACT could be as a prognostic biomarker for poor overall survival and DFS (\u003cspan citationid=\"CR37\" class=\"CitationRef\"\u003e37\u003c/span\u003e). Our results supported that increased PLR suggested poor efficacy of NACT.\u003c/p\u003e \u003cp\u003eSome researchers believed that cell component in blood might can provide information about the status of tumor microenvironment, since most inflammation and immune cells in tumor microenvironment were derived from peripheral blood (\u003cspan citationid=\"CR16\" class=\"CitationRef\"\u003e16\u003c/span\u003e). However, there are few studies to investigate the relationship between peripheral blood cells and corresponding infiltrating cells in tumor stroma. One important reason is the lack of a specific marker to label the same type of cells in both blood and stroma. P-selectin, also known as CD62P, is stored in α-granules and Weibel\u0026ndash;Palade bodies of platelets and endothelial cells, respectively. P-selectin expression is considered to be a marker of platelet activation. P-selectin on activated platelets can mediate interactions with monocytes, instigating paracrine-signaling mechanisms that lead to enhanced inflammation, as well as inducing factors that trigger thrombosis (\u003cspan citationid=\"CR38\" class=\"CitationRef\"\u003e38\u003c/span\u003e). In this study, P-selectin staining was performed on the specimens of 125 patients. The results showed that the expression of P-selectin in tumor microenvironment was significantly positively correlated with PLR, suggesting that the expression of P-selectin in stroma might be affected by platelets in peripheral blood, but the mechanism needs to be further studied.\u003c/p\u003e \u003cp\u003eThere are still some limitations in this study. First, this is a single-center retrospective study with a small sample size and the follow-up time is short, which makes some conclusions less convincing. Second, although P-selectin is thought to be expressed mainly on activated platelets, it is not a platelet-specific marker. The expression levels of P-selectin in tissues were not exactly equivalent to TEPs. Therefore, the significance of correlation analysis between P-selectin and PLR remains to be further explored.\u003c/p\u003e"},{"header":"Conclusion","content":"\u003cp\u003eThis study confirmed that systemic inflammatory-immune imbalance after NACT was associated with poor chemotherapy response, and PLR after chemotherapy could serve as a potential predictor. In addition, we found that the expression of P-selectin in tumor microenvironment might be affected by PLR in peripheral blood, but the exact mechanism needs to be further studied.\u003c/p\u003e"},{"header":"Methods","content":"\u003cdiv id=\"Sec10\" class=\"Section2\"\u003e \u003ch2\u003ePatients\u003c/h2\u003e \u003cp\u003eThis is a single-center retrospective study. The subjects included clinically diagnosed BC patients who received NACT followed by surgery at our hospital between January 2018 and September 2022. Inclusion criteria included: (\u003cspan citationid=\"CR1\" class=\"CitationRef\"\u003e1\u003c/span\u003e) age\u0026thinsp;\u0026gt;\u0026thinsp;18 years old; (\u003cspan citationid=\"CR2\" class=\"CitationRef\"\u003e2\u003c/span\u003e) invasive BC confirmed by biopsy; (\u003cspan citationid=\"CR3\" class=\"CitationRef\"\u003e3\u003c/span\u003e) at least immunohistochemical results for ER, PR, HER2, and Ki67 were available; (\u003cspan citationid=\"CR4\" class=\"CitationRef\"\u003e4\u003c/span\u003e) all patients should meet the NACT criteria according to established guidelines (\u003cspan citationid=\"CR23\" class=\"CitationRef\"\u003e23\u003c/span\u003e); (\u003cspan citationid=\"CR5\" class=\"CitationRef\"\u003e5\u003c/span\u003e) radical surgery for each patient performed at Nanjing Drum Tower Hospital after NACT. Exclusion criteria included: (\u003cspan citationid=\"CR1\" class=\"CitationRef\"\u003e1\u003c/span\u003e) patients with clinical stage IV or inflammatory breast cancer or history of previous malignant tumor; (\u003cspan citationid=\"CR2\" class=\"CitationRef\"\u003e2\u003c/span\u003e) clinicopathological data were insufficient; (\u003cspan citationid=\"CR3\" class=\"CitationRef\"\u003e3\u003c/span\u003e) patients who did not meet the NACT criteria or terminated chemotherapy prematurely; (\u003cspan citationid=\"CR4\" class=\"CitationRef\"\u003e4\u003c/span\u003e) patients who did not undergo radical surgery at Nanjing Drum Tower Hospital after NACT.\u003c/p\u003e \u003c/div\u003e \u003cdiv id=\"Sec11\" class=\"Section2\"\u003e \u003ch2\u003eEthical consideration\u003c/h2\u003e \u003cp\u003e This study was approved by the ethical and scientific committee of Nanjing Drum Tower Hospital (number 2023-460-02) and followed the principles of Declaration of Helsinki.\u003c/p\u003e \u003c/div\u003e \u003cdiv id=\"Sec12\" class=\"Section2\"\u003e \u003ch2\u003eData collection and processing\u003c/h2\u003e \u003cp\u003eMedical electronic records and pathology reports of patients were reviewed. The recorded information included: age, body mass index (BMI), menstrual status, clinical stage before chemotherapy (tumor size, axillary lymph node status, distant metastasis or not), pathological results of biopsy and radical surgical specimens, NACT regimens, imaging assessment results during NACT, and surgery methods.\u003c/p\u003e \u003cp\u003eHormone receptor (HR), HER2, and Ki67 index were characterized on biopsy and radical surgical specimens in the central laboratory of our hospital. HER2 was determined according to ASCO/CAP guidelines (\u003cspan citationid=\"CR24\" class=\"CitationRef\"\u003e24\u003c/span\u003e). HR\u0026thinsp;+\u0026thinsp;was defined if more than 1% of tumor cells were positive for ER/PR immunohistochemical staining. The absolute counts of neutrophils, monocytes, platelets and lymphocytes in peripheral blood of patients before the first NACT and after the last chemotherapy were recorded in an excel sheet. And then they were used to calculate NLR, MLR, and PLR.\u003c/p\u003e \u003c/div\u003e \u003cdiv id=\"Sec13\" class=\"Section2\"\u003e \u003ch2\u003eImmunohistochemistry\u003c/h2\u003e \u003cp\u003eParaffin sections were deparaffinized at 65℃ for 1 hour and treated in a pressure boiler with EDTA for 10 min. The sections were placed in 3% hydrogen peroxide solution and incubated at room temperature for 10 min to block endogenous peroxidase. Each section was washed 3 times with phosphate-buffered saline (PBS), sealed with Protein Block Serum-free for 20 min and then incubated with primary rabbit monoclonal antibody (Abcam, Cambridge, USA) overnight at 4\u0026ordm;C. After that, the sections were incubated with secondary antibody (Dako, California, USA) for 30 min, washed with PBS for 3 times. Tissues were stained for 5 min with fresh DAB solution and then counterstained with Mayer's hematoxylin and mounted in Permount (Fisher Scientific, New Jersey, USA). Samples were evaluated by one independent observer using an optical microscope. Only cancer cells with a distinct brown staining of the nucleus were considered positive.\u003c/p\u003e \u003c/div\u003e \u003cdiv id=\"Sec14\" class=\"Section2\"\u003e \u003ch2\u003eOutcomes\u003c/h2\u003e \u003cp\u003eThe primary outcome measured in this study was pCR. The pCR was defined by ypT0/Tis, N0, M0. Secondary outcome were disease-free survival (DFS) and overall survival (OS). DFS was defined as the time from radical surgery after NACT until any distant or local recurrence and OS was defined as the period from the date of diagnosis until the date of death assessed by Response Evaluation Criteria in Solid Tumors 1.1 (RECIST 1.1) (\u003cspan citationid=\"CR25\" class=\"CitationRef\"\u003e25\u003c/span\u003e).\u003c/p\u003e \u003c/div\u003e \u003cdiv id=\"Sec15\" class=\"Section2\"\u003e \u003ch2\u003eStatistical analysis\u003c/h2\u003e \u003cp\u003eThe statistical analysis was done using SPSS 20 (IBM Corp., Armonk, NY). Continuous variables were expressed as \"mean\u0026thinsp;\u0026plusmn;\u0026thinsp;standard error\", and categorical variables were expressed as percentage (%). Chi-square test or Fisher's exact test was used to compare the categorical parameters between the two groups. T-test were used to compare the normally distributed continuous variables. Correlation analysis of categorical parameters was done by Pearson χ\u0026sup2; test. The optimal cut-off value of each index was determined using receiver operating characteristic (ROC) curve analysis. Kaplan-Meier method was used to plot survival curve and log-rank test was used to compare survival difference. A p value\u0026thinsp;\u0026lt;\u0026thinsp;0.05 was considered statistically significant.\u003c/p\u003e "},{"header":"Declarations","content":"\u003ch2\u003eCompeting interests\u003c/h2\u003e \u003cp\u003eThe authors declare no competing interests.\u003c/p\u003e\u003ch2\u003eFunding\u003c/h2\u003e \u003cp\u003eThis work was supported by the Natural Science Foundation of Jiangsu Province [grant number BK20190126].\u003c/p\u003e\u003ch2\u003eAuthor Contribution\u003c/h2\u003e\u003cp\u003eW.W. designed the study, collected and analyzed the data, prepared tables and figures and drafted the first manuscript. H.C. and R.Z. collected the data. W.Z. provided critical comments for this paper. Y.Y. reviewed the final draft. All authors contributed to the article and approved the submitted version.\u003c/p\u003e\u003ch2\u003eData Availability\u003c/h2\u003e\u003cp\u003eThe dataset utilized and analyzed in the current research is accessible from the corresponding authors upon reasonable request.\u003c/p\u003e"},{"header":"References","content":"\u003col\u003e\u003cli\u003e\u003cspan\u003eGampenrieder SP, Rinnerthaler G, Greil R. Neoadjuvant Chemotherapy and Targeted Therapy in Breast Cancer: Past, Present, and Future. Journal of Oncology. 2013;2013:1\u0026ndash;12.\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eKong X, Moran MS, Zhang N, Haffty B, Yang Q. Meta-analysis confirms achieving pathological complete response after neoadjuvant chemotherapy predicts favourable prognosis for breast cancer patients. Eur J Cancer. 2011;47(14):2084\u0026ndash;90.\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eProvenzano E. 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Inflammatory Blood Markers as Prognostic and Predictive Factors in Early Breast Cancer Patients Receiving Neoadjuvant Chemotherapy. Cancers (Basel). 2020;12(9).\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eEthier JL, Desautels D, Templeton A, Shah PS, Amir E. Prognostic role of neutrophil-to-lymphocyte ratio in breast cancer: a systematic review and meta-analysis. Breast Cancer Res. 2017;19(1):2.\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eLusho S, Durando X, Mouret-Reynier M-A, Kossai M, Lacrampe N, Molnar I, et al. Platelet-to-Lymphocyte Ratio Is Associated With Favorable Response to Neoadjuvant Chemotherapy in Triple Negative Breast Cancer: A Study on 120 Patients. Frontiers in oncology. 2021;11:678315.\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eGianni C, Palleschi M, Schepisi G, Casadei C, Bleve S, Merloni F, et al. Circulating inflammatory cells in patients with metastatic breast cancer: Implications for treatment. Front Oncol. 2022;12:882896.\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eXue LB, Liu YH, Zhang B, Yang YF, Yang D, Zhang LW, et al. Prognostic role of high neutrophil-to-lymphocyte ratio in breast cancer patients receiving neoadjuvant chemotherapy: Meta-analysis. Medicine (Baltimore). 2019;98(1):e13842.\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eLi X, Dai D, Chen B, Tang H, Xie X, Wei W. The value of neutrophil-to-lymphocyte ratio for response and prognostic effect of neoadjuvant chemotherapy in solid tumors: A systematic review and meta-analysis. J Cancer. 2018;9(5):861\u0026ndash;71.\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eLi J, Jiang Z. Chinese Society of Clinical Oncology Breast Cancer (CSCO BC) guidelines in 2022: stratification and classification. Cancer Biol Med. 2022.\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eWolff AC, Hammond MEH, Allison KH, Harvey BE, Mangu PB, Bartlett JMS, et al. 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Clin Breast Cancer. 2020;20(4):e403-e9.\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eCuello-Lopez J, Fidalgo-Zapata A, Lopez-Agudelo L, Vasquez-Trespalacios E. Platelet-to-lymphocyte ratio as a predictive factor of complete pathologic response to neoadjuvant chemotherapy in breast cancer. PLoS One. 2018;13(11):e0207224.\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eZhang M, Huang XZ, Song YX, Gao P, Sun JX, Wang ZN. High Platelet-to-Lymphocyte Ratio Predicts Poor Prognosis and Clinicopathological Characteristics in Patients with Breast Cancer: A Meta-Analysis. Biomed Res Int. 2017;2017:9503025.\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eNasti TH, Bullard DC, Yusuf N. P-selectin enhances growth and metastasis of mouse mammary tumors by promoting regulatory T cell infiltration into the tumors. Life Sci. 2015;131:11\u0026ndash;8.\u003c/span\u003e\u003c/li\u003e\u003c/ol\u003e"},{"header":"Tables","content":"\u003cp\u003e \u003cdiv class=\"gridtable\"\u003e\u003ctable float=\"Yes\" id=\"Tab1\" border=\"1\"\u003e \u003ccaption language=\"En\"\u003e \u003cdiv class=\"CaptionNumber\"\u003eTable 1\u003c/div\u003e \u003cdiv class=\"CaptionContent\"\u003e \u003cp\u003eThe clinicopathological information of the patients. BMI: body mass index, TAC: docetaxel\u0026thinsp;+\u0026thinsp;Adriamycin\u0026thinsp;+\u0026thinsp;cyclophosphamide, TCbHP: docetaxel\u0026thinsp;+\u0026thinsp;carboplatin\u0026thinsp;+\u0026thinsp;trastuzumab\u0026thinsp;+\u0026thinsp;pertuzumab, TCbH: docetaxel\u0026thinsp;+\u0026thinsp;carboplatin\u0026thinsp;+\u0026thinsp;trastuzumab, THP: docetaxel\u0026thinsp;+\u0026thinsp;trastuzumab\u0026thinsp;+\u0026thinsp;pertuzumab, pCR: pathological complete response, MP: Miller-Payne grading system.\u003c/p\u003e \u003c/div\u003e \u003c/caption\u003e \u003ccolgroup cols=\"3\"\u003e \u003cdiv align=\"left\" class=\"colspec\" colname=\"c1\" colnum=\"1\"\u003e\u003c/div\u003e \u003cdiv align=\"char\" char=\".\" class=\"colspec\" colname=\"c2\" colnum=\"2\"\u003e\u003c/div\u003e \u003cdiv align=\"char\" char=\".\" class=\"colspec\" colname=\"c3\" colnum=\"3\"\u003e\u003c/div\u003e \u003cthead\u003e \u003ctr\u003e \u003cth align=\"left\" colname=\"c1\"\u003e\u0026nbsp;\u003c/th\u003e \u003cth align=\"left\" colname=\"c2\"\u003e \u003cp\u003eN (315)\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c3\"\u003e \u003cp\u003ePercentage (%)\u003c/p\u003e \u003c/th\u003e \u003c/tr\u003e \u003c/thead\u003e \u003ctbody\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eAge\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e\u0026nbsp;\u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e\u0026le;\u0026thinsp;35\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e \u003cp\u003e40\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e12.7\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e36\u0026ndash;55\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e \u003cp\u003e183\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e58.1\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e\u0026ge;\u0026thinsp;56\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e \u003cp\u003e92\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e29.2\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eBMI\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e\u0026nbsp;\u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e\u0026lt;\u0026thinsp;24\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e \u003cp\u003e165\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e52.38\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e\u0026ge;\u0026thinsp;24\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e \u003cp\u003e150\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e47.62\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eMenopausal status\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e\u0026nbsp;\u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003epre-menopausal\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e \u003cp\u003e170\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e53.97\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003epost-menopausal\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e \u003cp\u003e145\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e46.03\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eT stage\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e\u0026nbsp;\u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eT1\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e \u003cp\u003e54\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e17.14\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eT2\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e \u003cp\u003e218\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e69.21\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eT3-T4\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e \u003cp\u003e43\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e13.65\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eAxillary lymph node\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e\u0026nbsp;\u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003enegative\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e \u003cp\u003e41\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e13.02\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003epositive\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e \u003cp\u003e224\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e71.11\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003euncertain\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e \u003cp\u003e50\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e15.87\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eHistological type\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e\u0026nbsp;\u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003einvasive ductal carcinoma\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e \u003cp\u003e304\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e96.51\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eothers\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e \u003cp\u003e11\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e3.49\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eHistopathological grade\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e\u0026nbsp;\u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eI-II\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e \u003cp\u003e213\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e67.62\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eIII\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e \u003cp\u003e102\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e32.38\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eER\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e\u0026nbsp;\u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003epositive\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e \u003cp\u003e185\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e58.73\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003enegative\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e \u003cp\u003e130\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e41.27\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003ePR\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e\u0026nbsp;\u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003epositive\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e \u003cp\u003e152\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e48.25\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003enegative\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e \u003cp\u003e163\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e51.75\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eHER2\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e\u0026nbsp;\u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003epositive\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e \u003cp\u003e147\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e46.67\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003enegative\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e \u003cp\u003e168\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e53.33\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eKi67\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e\u0026nbsp;\u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e\u0026le;\u0026thinsp;14%\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e \u003cp\u003e11\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e3.49\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e\u0026gt;\u0026thinsp;14%\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e \u003cp\u003e304\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e96.51\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eChemotherapy regimens\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e\u0026nbsp;\u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eTAC \u0026times; 6\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e \u003cp\u003e161\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e51.11\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eTCbH/TCbHP \u0026times; 6\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e \u003cp\u003e114\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e36.19\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eTHP \u0026times; 6\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e \u003cp\u003e22\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e6.98\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eothers\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e \u003cp\u003e18\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e5.72\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003epCR\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e\u0026nbsp;\u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eyes\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e \u003cp\u003e104\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e33.02\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eno\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e \u003cp\u003e211\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e66.98\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003epT\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e\u0026nbsp;\u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eT0/Tis\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e \u003cp\u003e116\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e36.83\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eT\u0026thinsp;\u0026ge;\u0026thinsp;1\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e \u003cp\u003e199\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e63.17\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003epN\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e\u0026nbsp;\u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eN0\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e \u003cp\u003e175\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e55.56\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eN+\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e \u003cp\u003e140\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e44.44\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eMP\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e\u0026nbsp;\u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e4\u0026ndash;5\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e \u003cp\u003e160\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e50.79\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e1\u0026ndash;3\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e \u003cp\u003e155\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e49.21\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003c/tbody\u003e \u003c/colgroup\u003e \u003c/table\u003e\u003c/div\u003e \u003c/p\u003e \u003cp\u003e \u003cdiv class=\"gridtable\"\u003e\u003ctable float=\"Yes\" id=\"Tab2\" border=\"1\"\u003e \u003ccaption language=\"En\"\u003e \u003cdiv class=\"CaptionNumber\"\u003eTable 2\u003c/div\u003e \u003cdiv class=\"CaptionContent\"\u003e \u003cp\u003eComparisons of BIMs between non-pCR and pCR group. NLR: neutrophil to lymphocyte ratio, MLR: monocyte-lymphocyte ratio, PLR: platelet to lymphocyte ratio, NACT: neoadjuvant chemotherapy.\u003c/p\u003e \u003c/div\u003e \u003c/caption\u003e \u003ccolgroup cols=\"7\"\u003e \u003cdiv align=\"left\" class=\"colspec\" colname=\"c1\" colnum=\"1\"\u003e\u003c/div\u003e \u003cdiv align=\"left\" class=\"colspec\" colname=\"c2\" colnum=\"2\"\u003e\u003c/div\u003e \u003cdiv align=\"left\" class=\"colspec\" colname=\"c3\" colnum=\"3\"\u003e\u003c/div\u003e \u003cdiv align=\"left\" class=\"colspec\" colname=\"c4\" colnum=\"4\"\u003e\u003c/div\u003e \u003cdiv align=\"left\" class=\"colspec\" colname=\"c5\" colnum=\"5\"\u003e\u003c/div\u003e \u003cdiv align=\"left\" class=\"colspec\" colname=\"c6\" colnum=\"6\"\u003e\u003c/div\u003e \u003cdiv align=\"left\" class=\"colspec\" colname=\"c7\" colnum=\"7\"\u003e\u003c/div\u003e \u003cthead\u003e \u003ctr\u003e \u003cth align=\"left\" colname=\"c1\"\u003e\u0026nbsp;\u003c/th\u003e \u003cth align=\"left\" colspan=\"3\" nameend=\"c4\" namest=\"c2\"\u003e \u003cp\u003ePre-NACT\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colspan=\"3\" nameend=\"c7\" namest=\"c5\"\u003e \u003cp\u003ePost-NACT\u003c/p\u003e \u003c/th\u003e \u003c/tr\u003e \u003c/thead\u003e \u003ctbody\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003enon-pCR\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003epCR\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003ep\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003enon-pCR\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c6\"\u003e \u003cp\u003epCR\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c7\"\u003e \u003cp\u003ep\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eNLR\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003e2.22\u0026thinsp;\u0026plusmn;\u0026thinsp;0.07\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003e2.15\u0026thinsp;\u0026plusmn;\u0026thinsp;0.10\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003e0.56\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e3.11\u0026thinsp;\u0026plusmn;\u0026thinsp;0.17\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c6\"\u003e \u003cp\u003e2.58\u0026thinsp;\u0026plusmn;\u0026thinsp;0.19\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c7\"\u003e \u003cp\u003e\u003cb\u003e0.05\u003c/b\u003e\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eMLR\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003e0.21\u0026thinsp;\u0026plusmn;\u0026thinsp;0.01\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003e0.19\u0026thinsp;\u0026plusmn;\u0026thinsp;0.01\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003e0.15\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e0.43\u0026thinsp;\u0026plusmn;\u0026thinsp;0.02\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c6\"\u003e \u003cp\u003e0.32\u0026thinsp;\u0026plusmn;\u0026thinsp;0.03\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c7\"\u003e \u003cp\u003e\u003cb\u003e\u0026lt;\u0026thinsp;0.01\u003c/b\u003e\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003ePLR\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003e145\u0026thinsp;\u0026plusmn;\u0026thinsp;4.25\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003e138.7\u0026thinsp;\u0026plusmn;\u0026thinsp;4.56\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003e0.36\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e211.20\u0026thinsp;\u0026plusmn;\u0026thinsp;10.06\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c6\"\u003e \u003cp\u003e148.80\u0026thinsp;\u0026plusmn;\u0026thinsp;9.89\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c7\"\u003e \u003cp\u003e\u003cb\u003e\u0026lt;\u0026thinsp;0.01\u003c/b\u003e\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003c/tbody\u003e \u003c/colgroup\u003e \u003c/table\u003e\u003c/div\u003e \u003c/p\u003e \u003cp\u003e \u003cdiv class=\"gridtable\"\u003e\u003ctable float=\"Yes\" id=\"Tab3\" border=\"1\"\u003e \u003ccaption language=\"En\"\u003e \u003cdiv class=\"CaptionNumber\"\u003eTable 3\u003c/div\u003e \u003cdiv class=\"CaptionContent\"\u003e \u003cp\u003eROC curve analyses of BIMs for pCR. ROC: receiver operating characteristic, AUC: area under the curve, CI: confidence interval.\u003c/p\u003e \u003c/div\u003e \u003c/caption\u003e \u003ccolgroup cols=\"7\"\u003e \u003cdiv align=\"left\" class=\"colspec\" colname=\"c1\" colnum=\"1\"\u003e\u003c/div\u003e \u003cdiv align=\"char\" char=\".\" class=\"colspec\" colname=\"c2\" colnum=\"2\"\u003e\u003c/div\u003e \u003cdiv align=\"char\" char=\".\" class=\"colspec\" colname=\"c3\" colnum=\"3\"\u003e\u003c/div\u003e \u003cdiv align=\"char\" char=\".\" class=\"colspec\" colname=\"c4\" colnum=\"4\"\u003e\u003c/div\u003e \u003cdiv align=\"char\" char=\".\" class=\"colspec\" colname=\"c5\" colnum=\"5\"\u003e\u003c/div\u003e \u003cdiv align=\"char\" char=\".\" class=\"colspec\" colname=\"c6\" colnum=\"6\"\u003e\u003c/div\u003e \u003cdiv align=\"char\" char=\".\" class=\"colspec\" colname=\"c7\" colnum=\"7\"\u003e\u003c/div\u003e \u003cthead\u003e \u003ctr\u003e \u003cth align=\"left\" colname=\"c1\"\u003e\u0026nbsp;\u003c/th\u003e \u003cth align=\"left\" colname=\"c2\"\u003e \u003cp\u003eCut-off value\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c3\"\u003e \u003cp\u003eAUC\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c4\"\u003e \u003cp\u003e95% CI\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c5\"\u003e \u003cp\u003ep\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c6\"\u003e \u003cp\u003eSensitivity\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c7\"\u003e \u003cp\u003eSpecificity\u003c/p\u003e \u003c/th\u003e \u003c/tr\u003e \u003c/thead\u003e \u003ctbody\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eNLR\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e \u003cp\u003e3.03\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e0.57\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e0.51\u0026ndash;0.64\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e \u003cp\u003e\u003cb\u003e0.04\u003c/b\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e0.76\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e0.40\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eMLR\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e \u003cp\u003e0.29\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e0.67\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e0.60\u0026ndash;0.73\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e \u003cp\u003e\u003cb\u003e\u0026lt;\u0026thinsp;0.01\u003c/b\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e0.61\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e0.65\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003ePLR\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e \u003cp\u003e143.37\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e0.73\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e0.67\u0026ndash;0.79\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e \u003cp\u003e\u003cb\u003e\u0026lt;\u0026thinsp;0.01\u003c/b\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e0.65\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c7\"\u003e \u003cp\u003e0.73\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003c/tbody\u003e \u003c/colgroup\u003e \u003c/table\u003e\u003c/div\u003e \u003c/p\u003e \u003cp\u003e \u003cdiv class=\"gridtable\"\u003e\u003ctable float=\"Yes\" id=\"Tab4\" border=\"1\"\u003e \u003ccaption language=\"En\"\u003e \u003cdiv class=\"CaptionNumber\"\u003eTable 4\u003c/div\u003e \u003cdiv class=\"CaptionContent\"\u003e \u003cp\u003eUnivariate and multivariate logistic regression analysis for the predictors of pCR. OR: odds ratio\u003c/p\u003e \u003c/div\u003e \u003c/caption\u003e \u003ccolgroup cols=\"9\"\u003e \u003cdiv align=\"left\" class=\"colspec\" colname=\"c1\" colnum=\"1\"\u003e\u003c/div\u003e \u003cdiv align=\"left\" class=\"colspec\" colname=\"c2\" colnum=\"2\"\u003e\u003c/div\u003e \u003cdiv align=\"left\" class=\"colspec\" colname=\"c3\" colnum=\"3\"\u003e\u003c/div\u003e \u003cdiv align=\"left\" class=\"colspec\" colname=\"c4\" colnum=\"4\"\u003e\u003c/div\u003e \u003cdiv align=\"left\" class=\"colspec\" colname=\"c5\" colnum=\"5\"\u003e\u003c/div\u003e \u003cdiv align=\"left\" class=\"colspec\" colname=\"c6\" colnum=\"6\"\u003e\u003c/div\u003e \u003cdiv align=\"left\" class=\"colspec\" colname=\"c7\" colnum=\"7\"\u003e\u003c/div\u003e \u003cdiv align=\"left\" class=\"colspec\" colname=\"c8\" colnum=\"8\"\u003e\u003c/div\u003e \u003cdiv align=\"left\" class=\"colspec\" colname=\"c9\" colnum=\"9\"\u003e\u003c/div\u003e \u003cthead\u003e \u003ctr\u003e \u003cth align=\"left\" colname=\"c1\"\u003e\u0026nbsp;\u003c/th\u003e \u003cth align=\"left\" colspan=\"4\" nameend=\"c5\" namest=\"c2\"\u003e \u003cp\u003eUnivariate analysis\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colspan=\"4\" nameend=\"c9\" namest=\"c6\"\u003e \u003cp\u003eMultivariate analysis\u003c/p\u003e \u003c/th\u003e \u003c/tr\u003e \u003c/thead\u003e \u003ctbody\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\" morerows=\"1\" rowspan=\"2\"\u003e \u003cp\u003eOR\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colspan=\"2\" nameend=\"c4\" namest=\"c3\"\u003e \u003cp\u003e95%CI\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\" morerows=\"1\" rowspan=\"2\"\u003e \u003cp\u003ep\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c6\" morerows=\"1\" rowspan=\"2\"\u003e \u003cp\u003eOR\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colspan=\"2\" nameend=\"c8\" namest=\"c7\"\u003e \u003cp\u003e95%CI\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\" morerows=\"1\" rowspan=\"2\"\u003e \u003cp\u003ep\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003eLower\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003eUpper\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c7\"\u003e \u003cp\u003eLower\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003eUpper\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eAge (\u0026le;\u0026thinsp;45 \u003cem\u003evs\u003c/em\u003e\u0026thinsp;\u0026gt;\u0026thinsp;45)\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003e1.62\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003e0.96\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003e2.64\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e0.07\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c6\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"left\" colname=\"c7\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e\u0026nbsp;\u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eMenopausal status (pre vs post)\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003e1.51\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003e0.94\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003e2.42\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e0.09\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c6\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"left\" colname=\"c7\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e\u0026nbsp;\u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eBMI (\u0026lt;\u0026thinsp;24 \u003cem\u003evs\u003c/em\u003e\u0026thinsp;\u0026ge;\u0026thinsp;24)\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003e1.03\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003e0.64\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003e1.65\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e0.91\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c6\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"left\" colname=\"c7\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e\u0026nbsp;\u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eT (T1-2 \u003cem\u003evs\u003c/em\u003e T3-4)\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003e0.57\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003e0.27\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003e1.22\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e0.14\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c6\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"left\" colname=\"c7\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e\u0026nbsp;\u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eNode (negative \u003cem\u003evs\u003c/em\u003e positive)\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003e0.84\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003e0.42\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003e1.68\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e0.62\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c6\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"left\" colname=\"c7\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e\u0026nbsp;\u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eWHO (I-II \u003cem\u003evs\u003c/em\u003e III)\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003e1.41\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003e0.86\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003e2.31\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e0.17\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c6\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"left\" colname=\"c7\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e\u0026nbsp;\u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eER (negative \u003cem\u003evs\u003c/em\u003e positive)\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003e0.41\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003e0.25\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003e0.66\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e\u003cb\u003e\u0026lt;\u0026thinsp;0.01\u003c/b\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c6\"\u003e \u003cp\u003e0.63\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c7\"\u003e \u003cp\u003e0.32\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e1.24\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e0.18\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003ePR (negative \u003cem\u003evs\u003c/em\u003e positive)\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003e0.34\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003e0.21\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003e0.55\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e\u003cb\u003e\u0026lt;\u0026thinsp;0.01\u003c/b\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c6\"\u003e \u003cp\u003e0.51\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c7\"\u003e \u003cp\u003e0.26\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e1.02\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e0.06\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eHER2 (negative \u003cem\u003evs\u003c/em\u003e positive)\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003e5.35\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003e3.19\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003e8.99\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e\u003cb\u003e\u0026lt;\u0026thinsp;0.01\u003c/b\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c6\"\u003e \u003cp\u003e2.87\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c7\"\u003e \u003cp\u003e1.54\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e5.37\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e\u003cb\u003e\u0026lt;\u0026thinsp;0.01\u003c/b\u003e\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eKi67 (\u0026le;\u0026thinsp;14% \u003cem\u003evs\u003c/em\u003e\u0026thinsp;\u0026gt;\u0026thinsp;14%)\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003e1.33\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003e0.35\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003e5.11\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e0.68\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c6\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"left\" colname=\"c7\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e\u0026nbsp;\u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eNLR (\u0026le;\u0026thinsp;cut-off \u003cem\u003evs\u003c/em\u003e\u0026thinsp;\u0026gt;\u0026thinsp;cut-off)\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003e0.48\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003e0.28\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003e0.81\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e\u003cb\u003e\u0026lt;\u0026thinsp;0.01\u003c/b\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c6\"\u003e \u003cp\u003e1\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c7\"\u003e \u003cp\u003e0.52\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e1.92\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e0.99\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eMLR (\u0026le;\u0026thinsp;cut-off \u003cem\u003evs\u003c/em\u003e\u0026thinsp;\u0026gt;\u0026thinsp;cut-off)\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003e0.34\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003e0.21\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003e0.56\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e\u003cb\u003e\u0026lt;\u0026thinsp;0.01\u003c/b\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c6\"\u003e \u003cp\u003e0.97\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c7\"\u003e \u003cp\u003e0.5\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e1.9\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e0.93\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003ePLR (\u0026le;\u0026thinsp;cut-off \u003cem\u003evs\u003c/em\u003e\u0026thinsp;\u0026gt;\u0026thinsp;cut-off)\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003e0.2\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003e0.12\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003e0.33\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e\u003cb\u003e\u0026lt;\u0026thinsp;0.01\u003c/b\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c6\"\u003e \u003cp\u003e0.34\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c7\"\u003e \u003cp\u003e0.19\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c8\"\u003e \u003cp\u003e0.61\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c9\"\u003e \u003cp\u003e\u003cb\u003e\u0026lt;\u0026thinsp;0.01\u003c/b\u003e\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003c/tbody\u003e \u003c/colgroup\u003e \u003c/table\u003e\u003c/div\u003e \u003c/p\u003e \u003cp\u003e \u003cdiv class=\"gridtable\"\u003e\u003ctable float=\"Yes\" id=\"Tab5\" border=\"1\"\u003e \u003ccaption language=\"En\"\u003e \u003cdiv class=\"CaptionNumber\"\u003eTable 5\u003c/div\u003e \u003cdiv class=\"CaptionContent\"\u003e \u003cp\u003eCorrelation analysis between PLR and pCR rate in different molecular subtypes\u003c/p\u003e \u003c/div\u003e \u003c/caption\u003e \u003ccolgroup cols=\"6\"\u003e \u003cdiv align=\"left\" class=\"colspec\" colname=\"c1\" colnum=\"1\"\u003e\u003c/div\u003e \u003cdiv align=\"char\" char=\".\" class=\"colspec\" colname=\"c2\" colnum=\"2\"\u003e\u003c/div\u003e \u003cdiv align=\"char\" char=\".\" class=\"colspec\" colname=\"c3\" colnum=\"3\"\u003e\u003c/div\u003e \u003cdiv align=\"char\" char=\".\" class=\"colspec\" colname=\"c4\" colnum=\"4\"\u003e\u003c/div\u003e \u003cdiv align=\"char\" char=\".\" class=\"colspec\" colname=\"c5\" colnum=\"5\"\u003e\u003c/div\u003e \u003cdiv align=\"char\" char=\".\" class=\"colspec\" colname=\"c6\" colnum=\"6\"\u003e\u003c/div\u003e \u003cthead\u003e \u003ctr\u003e \u003cth align=\"left\" colname=\"c1\"\u003e\u0026nbsp;\u003c/th\u003e \u003cth align=\"left\" colname=\"c2\"\u003e \u003cp\u003e\u0026le; cut-off\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c3\"\u003e \u003cp\u003e\u0026gt; cut-off\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c4\"\u003e \u003cp\u003eOR\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c5\"\u003e \u003cp\u003e95%CI\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c6\"\u003e \u003cp\u003ep\u003c/p\u003e \u003c/th\u003e \u003c/tr\u003e \u003c/thead\u003e \u003ctbody\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eLuminal A/B\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e0.46\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e \u003cp\u003e0.24\u0026ndash;0.86\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e\u003cb\u003e0.02\u003c/b\u003e\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003enon-pCR\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e \u003cp\u003e45\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e99\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"left\" colname=\"c6\"\u003e\u0026nbsp;\u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003epCR\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e \u003cp\u003e27\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e27\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"left\" colname=\"c6\"\u003e\u0026nbsp;\u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eHER2+ (ER-, PR-)\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e0.32\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e \u003cp\u003e0.11\u0026ndash;0.94\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e\u003cb\u003e0.04\u003c/b\u003e\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003enon-pCR\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e \u003cp\u003e13\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e14\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"left\" colname=\"c6\"\u003e\u0026nbsp;\u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003epCR\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e \u003cp\u003e26\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e9\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"left\" colname=\"c6\"\u003e\u0026nbsp;\u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eTNBC\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e \u003cp\u003e0.24\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e \u003cp\u003e0.07\u0026ndash;0.89\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e \u003cp\u003e\u003cb\u003e0.04\u003c/b\u003e\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003enon-pCR\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e \u003cp\u003e7\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e33\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"left\" colname=\"c6\"\u003e\u0026nbsp;\u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003epCR\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e \u003cp\u003e7\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e \u003cp\u003e8\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"left\" colname=\"c6\"\u003e\u0026nbsp;\u003c/td\u003e \u003c/tr\u003e \u003c/tbody\u003e \u003c/colgroup\u003e \u003c/table\u003e\u003c/div\u003e \u003c/p\u003e \u003cp\u003eTNBC: triple negative breast cancer\u003c/p\u003e \u003cp\u003e \u003c/p\u003e \u003cp\u003e \u003cdiv class=\"gridtable\"\u003e\u003ctable float=\"Yes\" id=\"Tab6\" border=\"1\"\u003e \u003ccaption language=\"En\"\u003e \u003cdiv class=\"CaptionNumber\"\u003eTable 6\u003c/div\u003e \u003cdiv class=\"CaptionContent\"\u003e \u003cp\u003eSurvival curves obtained with Kaplan-Meier analysis. DFS: disease-free survival, OS: overall survival\u003c/p\u003e \u003c/div\u003e \u003c/caption\u003e \u003ccolgroup cols=\"7\"\u003e \u003cdiv align=\"left\" class=\"colspec\" colname=\"c1\" colnum=\"1\"\u003e\u003c/div\u003e \u003cdiv align=\"left\" class=\"colspec\" colname=\"c2\" colnum=\"2\"\u003e\u003c/div\u003e \u003cdiv align=\"left\" class=\"colspec\" colname=\"c3\" colnum=\"3\"\u003e\u003c/div\u003e \u003cdiv align=\"left\" class=\"colspec\" colname=\"c4\" colnum=\"4\"\u003e\u003c/div\u003e \u003cdiv align=\"left\" class=\"colspec\" colname=\"c5\" colnum=\"5\"\u003e\u003c/div\u003e \u003cdiv align=\"left\" class=\"colspec\" colname=\"c6\" colnum=\"6\"\u003e\u003c/div\u003e \u003cdiv align=\"left\" class=\"colspec\" colname=\"c7\" colnum=\"7\"\u003e\u003c/div\u003e \u003cthead\u003e \u003ctr\u003e \u003cth align=\"left\" colname=\"c1\"\u003e\u0026nbsp;\u003c/th\u003e \u003cth align=\"left\" colspan=\"3\" nameend=\"c4\" namest=\"c2\"\u003e \u003cp\u003eDFS(\u0026le;\u0026thinsp;cut-off \u003cem\u003evs\u003c/em\u003e\u0026thinsp;\u0026gt;\u0026thinsp;cut-off)\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colspan=\"3\" nameend=\"c7\" namest=\"c5\"\u003e \u003cp\u003eOS(\u0026le;\u0026thinsp;cut-off \u003cem\u003evs\u003c/em\u003e\u0026thinsp;\u0026gt;\u0026thinsp;cut-off)\u003c/p\u003e \u003c/th\u003e \u003c/tr\u003e \u003c/thead\u003e \u003ctbody\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e\u0026nbsp;\u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003eratio\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003e95% CI\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003ep\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003eratio\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c6\"\u003e \u003cp\u003e95% CI\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c7\"\u003e \u003cp\u003ep\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eNLR\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003e1.06\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003e0.50\u0026ndash;2.27\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003e0.88\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e1.22\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c6\"\u003e \u003cp\u003e0.23\u0026ndash;6.32\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c7\"\u003e \u003cp\u003e0.82\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eMLR\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003e1.40\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003e0.66\u0026ndash;2.94\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003e0.38\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e0.98\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c6\"\u003e \u003cp\u003e0.18\u0026ndash;5.33\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c7\"\u003e \u003cp\u003e0.98\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003ePLR\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003e0.95\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003e0.45\u0026ndash;2.02\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003e0.89\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e1.12\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c6\"\u003e \u003cp\u003e0.19\u0026ndash;6.43\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c7\"\u003e \u003cp\u003e0.90\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003c/tbody\u003e \u003c/colgroup\u003e \u003c/table\u003e\u003c/div\u003e \u003c/p\u003e \u003c/div\u003e"}],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":true,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":true,"highlight":"","institution":"","isAcceptedByJournal":false,"isAuthorSuppliedPdf":false,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":true,"isPdf":false,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true},"keywords":"Breast cancer, Neoadjuvant chemotherapy, Platelet to lymphocyte ratio, Chemotherapy response","lastPublishedDoi":"10.21203/rs.3.rs-4692507/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-4692507/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003eIn this study, our aim was to explore the correlation between blood inflammatory markers (BIMs), including neutrophil to lymphocyte ratio (NLR), monocyte to lymphocyte ratio (MLR) and platelet to lymphocyte ratio (PLR), and the efficacy of neoadjuvant chemotherapy (NACT) in BC patients. Besides, the relationship between tumor infiltrating inflammatory cells (TIIs) and BIMs has also been preliminarily studied. A total of 315 BC patients between January 2018 and September 2022 were analyzed. Receiver operating characteristic curve was used to determine the cut-off value of each BIM. Logistic regression analysis were used to evaluated the predictive power of BIMs for pathological complete response (pCR). TIIs were assessed by immunohistochemistry, and their relationships with BIMs also were analyzed. The results showed that NLR, MLR, PLR in the pCR group were significantly lower than those in the non-pCR group after NACT (p\u0026thinsp;\u0026le;\u0026thinsp;0.05). PLR had the largest area under the curve (AUC) (0.73) compared to NLR (0.57) and MLR (0.67) (p\u0026thinsp;\u0026lt;\u0026thinsp;0.01). Univariate analysis showed ER, PR, HER2, NLR, MLR, PLR were significantly associated with pCR, but multivariate analysis found only HER2 and PLR were independently predictors for pCR (p\u0026thinsp;\u0026lt;\u0026thinsp;0.01). PLR was positively correlated with the expression of P-selectin in tumor tissue (r: 0.26, p\u0026thinsp;\u0026lt;\u0026thinsp;0.01). Survival analysis showed that NLR, MLR, PLR had no significant correlation with disease prognosis. In conclusion, PLR after NACT could serve as a predictor for pCR, and it correlated with the imbalance of the tumor-infiltrating platelet to lymphocyte ratio, which might cause drug resistance.\u003c/p\u003e","manuscriptTitle":"High platelet - lymphocyte ratio may predict poor therapeutic response in breast cancer patients treated with neoadjuvant chemotherapy","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2024-08-09 20:51:10","doi":"10.21203/rs.3.rs-4692507/v1","editorialEvents":[{"type":"communityComments","content":0}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"36afd6c4-3df8-48be-8d8c-652bb2a3b9cd","owner":[],"postedDate":"August 9th, 2024","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"posted","subjectAreas":[{"id":35343974,"name":"Biological sciences/Cancer/Breast cancer"},{"id":35343975,"name":"Health sciences/Biomarkers/Predictive markers"},{"id":35343976,"name":"Biological sciences/Cancer/Cancer therapy/Chemotherapy"}],"tags":[],"updatedAt":"2024-09-13T04:17:29+00:00","versionOfRecord":[],"versionCreatedAt":"2024-08-09 20:51:10","video":"","vorDoi":"","vorDoiUrl":"","workflowStages":[]},"version":"v1","identity":"rs-4692507","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-4692507","identity":"rs-4692507","version":["v1"]},"buildId":"qtupq5eGEP_6zYnWcrvyt","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}