REVS: A New Open-Source Platform for High-Resolution Analysis of Rodent Wheel Running Behavior

Background Rodent wheel running is widely used in neuroscience and preclinical research to assess locomotor function, recovery post-trauma or disease, circadian rhythms, and exercise physiology. However, most existing wheel-running systems offer limited metrics, lack flexibility in hardware, or require costly proprietary software, reducing their usefulness for detailed behavioral phenotyping—especially in models of injury or rehabilitation. New method We developed REVS (Revolution Evaluation and Visualization Software), a low-cost, open-source hardware and software platform for analyzing and visualizing rodent wheel running behavior. REVS captures wheel revolutions using Hall effect sensors and computes 13 day-level behavioral metrics along with detailed bout-level data. Users can interactively explore high-resolution temporal features and export data in Open Data Commons (ODC)-compatible formats. REVS supports customizable wheel types, facilitating use in animals with motor and/or sensory impairments. Results We validated REVS using a mouse model of partial spinal cord injury, where fine motor control is compromised. REVS detected impairments in 10 of 13 behavioral metrics post-injury, with varied recovery trajectories across measures. Principal component analysis revealed that recovery was closely linked to bout quality and intensity, rather than timing. Comparison with existing methods Unlike commercial and open-source systems, REVS offers more detailed metrics, customizable wheel compatibility, seamless blending with common vivarium hardware, integrated data visualizations, and ODC-compatible data export. It also supports flexible analysis across individuals and groups. Conclusions REVS provides a powerful, scalable tool for granular behavioral phenotyping in rodent studies, enhancing reproducibility and revealing insights into subtle locomotor changes associated with injury, recovery, and intervention. Highlights REVS enables detailed analysis of voluntary wheel running in rodents The platform combines low-cost hardware with open-source analysis software REVS computes 13 behavioral metrics across daily and bout-level timescales We identified a unique lesion and recovery profile after partial spinal cord injury REVS supports ODC-compatible data export for transparency and reuse