Intranasal Colonization of Germ-Free Mice with Human Nasal Microbial Communities

2026 · doi:10.64898/2026.04.24.720711
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Abstract

We present a novel protocol for intranasal colonization of germ-free mice with human nasal microbiota, coupled with an optimized DNA extraction method for murine nasal samples compatible with 16S rRNA sequencing. This protocol may facilitate investigation of the functional roles and causal effects of nasal microbial communities in chronic rhinosinusitis and antimicrobial resistance.
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Abstract We present a novel protocol for intranasal colonization of germ-free mice with human nasal microbiota, coupled with an optimized DNA extraction method for murine nasal samples compatible with 16S rRNA sequencing. This protocol may facilitate investigation of the functional roles and causal effects of nasal microbial communities in chronic rhinosinusitis and antimicrobial resistance. Competing Interest Statement The authors have declared no competing interest. Funder Information Declared Ministry of Education, Youth and Sports of the Czech Republic, CZ.02.01.01/00/22_008/0004597 Czech Science Foundation, https://ror.org/01pv73b02, 26-19874L National Science Centre, UMO-2024/55/I/NZ7/02334 Copyright The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.

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