CRH, UCN, CRHR1 and CRHR2 excessive expression in endometriotic sites compared to eutopic endometrium of endometriotic women.

3a) A: mRNA expression of CRH (413 bps), UCN (146 bps), CRHR1β (554 bps), CRHR2α (322 bps), GAPDH (483 bps) in endometriotic tissue (e) and the corresponding eutopic endometrium (ee). B: Immunohistochemical expression of CRH(A,D) and UCN(B,E) in eutopic(A,B) and ectopic endometrium(D,E). Both CRH and UCN are mainly expressed in endometriotic lesions.(C: negative eutopic endometrium, F: negative ectopic endometrium). C: Western blot immunodetection of CRHR1 (55 KDa) and CRHR2 (55 KDa), in endometriotic tissue(e) and eutopic endometrium(ee) of endometriotic women.GAPDH (37 kDa) used as a house keeping gene. CRH, UCN, CRHR1 and CRHR2α are significantly more expressed at an mRNA and protein level in endometriotic tissue compared to the corresponding eutopic endometrium. (representative data). 3b) Presentation of the normalized fold-increase of CRH, UCN, CRHR1 and CRHR2 expression in the study population at mRNA level(A) and protein level(B: CRH and UCN protein levels, C: CRHR1 and CRHR2 protein levels) (*, p<0.05, **,p<0.01).