Comparative genomic analysis of co-occurring hybrid zones of house mouse parasites Pneumocystis murina and Syphacia obvelata using genome polarisation

Comparative genomic analysis of co-occurring hybrid zones of house mouse parasites Pneumocystis murina and Syphacia obvelata using genome polarisation | Authorea try { document.documentElement.classList.add('js'); } catch (e) { } var _gaq = _gaq || []; _gaq.push(['_setAccount', 'G-8VDV14Y67G']); _gaq.push(['_trackPageview']); (function() { var ga = document.createElement('script'); ga.type = 'text/javascript'; ga.async = true; ga.src = ('https:' == document.location.protocol ? 'https://ssl' : 'http://www') + '.google-analytics.com/ga.js'; var s = document.getElementsByTagName('script')[0]; s.parentNode.insertBefore(ga, s); })(); Skip to main content Preprints Collections Wiley Open Research IET Open Research Ecological Society of Japan All Collections About About Authorea FAQs Contact Us Quick Search anywhere Search for preprint articles, keywords, etc. Search Search ADVANCED SEARCH SCROLL Molecular Ecology This is a preprint and has not been peer reviewed. Data may be preliminary. 31 January 2025 V1 Latest version Share on Comparative genomic analysis of co-occurring hybrid zones of house mouse parasites Pneumocystis murina and Syphacia obvelata using genome polarisation Authors : Jan Petružela 0009-0005-8267-2592 [email protected] , Beate Nürnberger , Alexis Ribas , Georgios Koutsovoulos , Dagmar Cizkova 0000-0001-5031-7792 , A Fornuskova , Tatiana Aghová , Mark Blaxter , Joëlle Goüy de Bellocq 0000-0001-5831-6284 , and Stuart Baird 0000-0002-7144-9919 Authors Info & Affiliations https://doi.org/10.22541/au.173833166.66086561/v1 Published Molecular Ecology Version of record Peer review timeline 362 views 190 downloads Contents Abstract Supplementary Material Information & Authors Metrics & Citations View Options References Figures Tables Media Share Abstract Parasites are expected to hybridise in similar ways to free living organisms, although this may be modified by their reduced genome architecture. Recombinant strains and taxa of hybrid origin can be studied in nature where hosts come into secondary contact. Here we apply genome-wide analysis to parasites from a contact previously characterised for many individuals using classical markers. The host contact is the European house mouse hybrid zone; the parasites are lung fungus Pneumocystis and gut pinworm Syphacia. The multilocus and multi-individual datasets are broadly consistent in scale and centering. Whole mitogenome comparisons confirm earlier suggestions that parasite divergence is low compared to their hosts, perhaps due to reduced genome stabilising selection. In the recombining genome we are able to show blocks of parasite genome of alternating host origin, including one Pneumocystis strain which appears to be an F3+ cross and one recombinant Syphacia strain found over multiple localities. Functional analyses of introgressing genes show enrichment for genes likely important for parasitic lifestyle. Supplementary Material File (fig10_concordance_diagrams.pdf) Download 363.16 KB File (manuscript_-_final_version_-_fin (1).doc) Download 3.42 MB File (table1_assembly_stats.xlsx) Download 5.33 KB File (table2_genic_content.xlsx) Download 5.58 KB File (table3_category_content.xlsx) Download 7.06 KB File (table4_pneumocystis_contingency_table.xlsx) Download 6.25 KB File (table5_syphacia_contingency_table.xlsx) Download 6.38 KB File (table6_intbarr_ratios.xlsx) Download 5.75 KB Information & Authors Information Version history V1 Version 1 31 January 2025 Peer review timeline Published Molecular Ecology Version of Record 14 Oct 2025 Published Copyright This work is licensed under a Non Exclusive No Reuse License. Collection Molecular Ecology Keywords pneumocystis murina syphacia obvelata adaptive introgression genome polarisation parasite hybrid zones population genomics Authors Affiliations Jan Petružela 0009-0005-8267-2592 [email protected] Masaryk University Faculty of Science View all articles by this author Beate Nürnberger Institute of Vertebrate Biology CAS View all articles by this author Alexis Ribas University of Barcelona View all articles by this author Georgios Koutsovoulos Foundation of Research and Technology Hellas View all articles by this author Dagmar Cizkova 0000-0001-5031-7792 Institute of Vertebrate Biology Czech Academy of Sciences View all articles by this author A Fornuskova Institute of Vertebrate Biology CAS View all articles by this author Tatiana Aghová Institute of Vertebrate Biology Czech Academy of Sciences View all articles by this author Mark Blaxter Wellcome Sanger Institute View all articles by this author Joëlle Goüy de Bellocq 0000-0001-5831-6284 Institute of Vertebrate Biology Czech Academy of Sciences View all articles by this author Stuart Baird 0000-0002-7144-9919 Institute of Vertebrate Biology Czech Academy of Sciences View all articles by this author Metrics & Citations Metrics Article Usage 362 views 190 downloads .FvxKWukQNSOunydq8rnd { width: 100px; } Citations Download citation Jan Petružela, Beate Nürnberger, Alexis Ribas, et al. Comparative genomic analysis of co-occurring hybrid zones of house mouse parasites Pneumocystis murina and Syphacia obvelata using genome polarisation. Authorea . 31 January 2025. DOI: https://doi.org/10.22541/au.173833166.66086561/v1 If you have the appropriate software installed, you can download article citation data to the citation manager of your choice. Simply select your manager software from the list below and click Download. For more information or tips please see 'Downloading to a citation manager' in the Help menu . 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