Qing-Chang-Hua-Shi Granule Attenuates Experimental Colitis via Lactobacillus gasseri-Mediated Suppression of Ferroptosis

Qing-Chang-Hua-Shi Granule Attenuates Experimental Colitis via Lactobacillus gasseri-Mediated Suppression of Ferroptosis | Authorea try { document.documentElement.classList.add('js'); } catch (e) { } var _gaq = _gaq || []; _gaq.push(['_setAccount', 'G-8VDV14Y67G']); _gaq.push(['_trackPageview']); (function() { var ga = document.createElement('script'); ga.type = 'text/javascript'; ga.async = true; ga.src = ('https:' == document.location.protocol ? 'https://ssl' : 'http://www') + '.google-analytics.com/ga.js'; var s = document.getElementsByTagName('script')[0]; s.parentNode.insertBefore(ga, s); })(); Skip to main content Preprints Collections Wiley Open Research IET Open Research Ecological Society of Japan All Collections About About Authorea FAQs Contact Us Quick Search anywhere Search for preprint articles, keywords, etc. Search Search ADVANCED SEARCH SCROLL This is a preprint and has not been peer reviewed. Data may be preliminary. 13 March 2025 V1 Latest version Share on Qing-Chang-Hua-Shi Granule Attenuates Experimental Colitis via Lactobacillus gasseri-Mediated Suppression of Ferroptosis Authors : Cheng Cheng , Lei Zhu , Jingyi Hu , Ziyu Lian , Weiyang Li , Ryan Au , Yanan Li , … Show All … , Feng Xu , Yuguang Wu , Yuan Cui , Zhe Di , Bin Li , Lilin Ge 0000-0001-8129-8926 [email protected] , Yao Lin , and Hong Shen Show Fewer Authors Info & Affiliations https://doi.org/10.22541/au.174188548.82334781/v1 148 views 91 downloads Contents Abstract Supplementary Material Information & Authors Metrics & Citations View Options References Figures Tables Media Share Abstract Ulcerative colitis (UC) is a chronic idiopathic inflammatory bowel disorder characterized by complex polygenic inheritance and refractory clinical manifestations. As one of the most challenging gastrointestinal conditions with unclear etiology, UC frequently exhibits persistent mucosal inflammation extending from the rectum to proximal colon regions. The limited efficacy of existing pharmacological regimens, coupled with its recurrent and treatment-resistant progression, highlights an imperative demand for advanced therapeutic innovations targeting this multifactorial pathology. Qing-Chang-Hua-Shi granule (QCHS), a traditional Chinese medicine for the treatment of UC, which has been widely used in clinical practice. Here, it is shown that QCHS improves the inflammatory response and intestinal barrier in DSS-induced colitic mice by regulating gut microbiota and their secreted metabolites. Specifically, QCHS increased the relative abundance of Lactobacillus gasseri ( L. gasseri ) and altered the composition of metabolite in feces. Further analysis showed that the metabolites altered by QCHS were significantly enriched in the ferroptosis metabolic pathway. QCHS can also promote the proliferation of L. gasseri in vitro and effectively repair ferroptosis-like injury in the colon of UC mice. Overall, these results indicate that QCHS is an effective agent against UC mainly through the regulation of ferroptosis associated metabolic pathway by modulating Lactobacillus gasseri novelty. Supplementary Material File (qing-chang-hua-shi granule.doc) Download 68.66 MB Information & Authors Information Version history V1 Version 1 13 March 2025 Copyright This work is licensed under a Non Exclusive No Reuse License. Keywords colitis lactobacillus gasseri qing-chang-hua-shi granule Authors Affiliations Cheng Cheng Nanjing University of Chinese Medicine View all articles by this author Lei Zhu Jiangsu Province Hospital of Chinese Medicine View all articles by this author Jingyi Hu Jiangsu Province Hospital of Chinese Medicine View all articles by this author Ziyu Lian Jiangsu Province Hospital of Chinese Medicine View all articles by this author Weiyang Li Jiangsu Province Hospital of Chinese Medicine View all articles by this author Ryan Au Jiangsu Province Hospital of Chinese Medicine View all articles by this author Yanan Li Jiangsu Province Hospital of Chinese Medicine View all articles by this author Feng Xu Jiangsu Province Hospital of Chinese Medicine View all articles by this author Yuguang Wu Jiangsu Province Hospital of Chinese Medicine View all articles by this author Yuan Cui Jiangsu Province Hospital of Chinese Medicine View all articles by this author Zhe Di Nanjing University of Chinese Medicine View all articles by this author Bin Li Nanjing University of Chinese Medicine View all articles by this author Lilin Ge 0000-0001-8129-8926 [email protected] Nanjing University of Chinese Medicine View all articles by this author Yao Lin Fujian University of Traditional Chinese Medicine View all articles by this author Hong Shen Jiangsu Province Hospital of Chinese Medicine View all articles by this author Metrics & Citations Metrics Article Usage 148 views 91 downloads .FvxKWukQNSOunydq8rnd { width: 100px; } Citations Download citation Cheng Cheng, Lei Zhu, Jingyi Hu, et al. Qing-Chang-Hua-Shi Granule Attenuates Experimental Colitis via Lactobacillus gasseri-Mediated Suppression of Ferroptosis. Authorea . 13 March 2025. DOI: https://doi.org/10.22541/au.174188548.82334781/v1 If you have the appropriate software installed, you can download article citation data to the citation manager of your choice. Simply select your manager software from the list below and click Download. For more information or tips please see 'Downloading to a citation manager' in the Help menu . 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