Mycorrhizal synthesis and physiological responses of Entoloma clypeatum and Malus robusta

Mycorrhizal synthesis and physiological responses of Entoloma clypeatum and Malus robusta | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Mycorrhizal synthesis and physiological responses of Entoloma clypeatum and Malus robusta Chen Hao, Jianrui Wang, Lunhe You, Qianwen Tan, Xiaoran Chen, and 2 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-3360728/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Entoloma clypeatum is an edible ectomycorrhizal fungus that is often symbiotic with Rosaceae fruit trees. Malus domestica is one of the main economic fruit trees in China. The symbiotic culture of E. clypeatum and M. domestica rootstock, Malus robusta for mycorrhizal synthesis was carried out. The mycorrhizal infection rate, biomass, chlorophyll content, photosynthetic index, plant hormone content, antioxidant enzyme activity and osmotic adjustment substance content of mycorrhizal seedlings and non-mycorrhizal seedlings seedlings were determined by morphological observation of mycorrhizal roots. The effects of E. clypeatum on symbiotic plants were expounded, which provided a basis for further research and application of the fungus. The results showed that E. clypeatum and M. robusta could form cylindrical or clavate mycorrhizal (52.5%), which could increase the biomass of symbiotic plants. The chlorophyll content increased by 95.4% compared with the control group, which improved the photosynthesis of seedlings. The contents of ZT, IAA and GA3 in roots, stems and leaves of plants were significantly higher than those in the uninoculated group (30.7%-186.45%), ABA content decreased (11.5-76.2%), antioxidant enzyme content increased (13.1%-111.5%), soluble protein, soluble sugar and free proline content increased, and malondialdehyde content decreased. The results showed that E. clypeatum had a positive effect on the growth of M. robusta and could enhance its adaptability and stress resistance. This also lays a foundation for the development and application of E. clypeatum and promotes the development of fruit tree industry, and provides a reference for the study of ectomycorrhizal fungi. Ectomycorrhizal fungi mycorrhizal morphology plant hormones photosynthesis antioxidant enzymes osmotic regulating substances Full Text Additional Declarations No competing interests reported. Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-3360728","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":234474087,"identity":"668b40a9-ec63-4f98-861d-2cd1e2ca5995","order_by":0,"name":"Chen Hao","email":"","orcid":"","institution":"Ludong University","correspondingAuthor":false,"submittingAuthor":false,"prefix":"","firstName":"Chen","middleName":"","lastName":"Hao","suffix":""},{"id":234474088,"identity":"f1fa057f-4230-4f93-9c06-5e9289685477","order_by":1,"name":"Jianrui Wang","email":"","orcid":"","institution":"Ludong 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morphology, plant hormones, photosynthesis, antioxidant enzymes, osmotic regulating substances","lastPublishedDoi":"10.21203/rs.3.rs-3360728/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-3360728/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"Entoloma clypeatum is an edible ectomycorrhizal fungus that is often symbiotic with Rosaceae fruit trees. Malus domestica is one of the main economic fruit trees in China. The symbiotic culture of E. clypeatum and M. domestica rootstock, Malus robusta for mycorrhizal synthesis was carried out. The mycorrhizal infection rate, biomass, chlorophyll content, photosynthetic index, plant hormone content, antioxidant enzyme activity and osmotic adjustment substance content of mycorrhizal seedlings and non-mycorrhizal seedlings seedlings were determined by morphological observation of mycorrhizal roots. The effects of E. clypeatum on symbiotic plants were expounded, which provided a basis for further research and application of the fungus. The results showed that E. clypeatum and M. robusta could form cylindrical or clavate mycorrhizal (52.5%), which could increase the biomass of symbiotic plants. The chlorophyll content increased by 95.4% compared with the control group, which improved the photosynthesis of seedlings. The contents of ZT, IAA and GA3 in roots, stems and leaves of plants were significantly higher than those in the uninoculated group (30.7%-186.45%), ABA content decreased (11.5-76.2%), antioxidant enzyme content increased (13.1%-111.5%), soluble protein, soluble sugar and free proline content increased, and malondialdehyde content decreased. The results showed that E. clypeatum had a positive effect on the growth of M. robusta and could enhance its adaptability and stress resistance. This also lays a foundation for the development and application of E. clypeatum and promotes the development of fruit tree industry, and provides a reference for the study of ectomycorrhizal fungi.","manuscriptTitle":"Mycorrhizal synthesis and physiological responses of Entoloma clypeatum and Malus robusta","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2023-09-25 06:23:21","doi":"10.21203/rs.3.rs-3360728/v1","editorialEvents":[{"type":"communityComments","content":0}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"dd27f5fc-bd8a-4181-9fb9-d34c696e2b8f","owner":[],"postedDate":"September 25th, 2023","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"posted","subjectAreas":[],"tags":[],"updatedAt":"2023-10-23T12:29:34+00:00","versionOfRecord":[],"versionCreatedAt":"2023-09-25 06:23:21","video":"","vorDoi":"","vorDoiUrl":"","workflowStages":[]},"version":"v1","identity":"rs-3360728","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-3360728","identity":"rs-3360728","version":["v1"]},"buildId":"WrCJVZZCHTDjtuVLN7oU0","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}