Effect of autophagy on pyocyanin-induced epithelial-mesenchymal transition (EMT) in alveolar epithelial cells

Effect of autophagy on pyocyanin-induced epithelial-mesenchymal transition (EMT) in alveolar epithelial cells | Authorea try { document.documentElement.classList.add('js'); } catch (e) { } var _gaq = _gaq || []; _gaq.push(['_setAccount', 'G-8VDV14Y67G']); _gaq.push(['_trackPageview']); (function() { var ga = document.createElement('script'); ga.type = 'text/javascript'; ga.async = true; ga.src = ('https:' == document.location.protocol ? 'https://ssl' : 'http://www') + '.google-analytics.com/ga.js'; var s = document.getElementsByTagName('script')[0]; s.parentNode.insertBefore(ga, s); })(); Skip to main content Preprints Collections Wiley Open Research IET Open Research Ecological Society of Japan All Collections About About Authorea FAQs Contact Us Quick Search anywhere Search for preprint articles, keywords, etc. Search Search ADVANCED SEARCH SCROLL This is a preprint and has not been peer reviewed. Data may be preliminary. 16 January 2025 V1 Latest version Share on Effect of autophagy on pyocyanin-induced epithelial-mesenchymal transition (EMT) in alveolar epithelial cells Authors : Li-zhuoyue [email protected] , Wang-jiali , and Ye-huan Authors Info & Affiliations https://doi.org/10.22541/au.173698923.36806423/v1 150 views 65 downloads Contents Abstract Supplementary Material Information & Authors Metrics & Citations View Options References Figures Tables Media Share Abstract Objective: Epithelial-Mesenchymal Transition (EMT) plays an important role in the occurrence and development of pulmonary fibrosis and can lead to severe cell damage. Autophagy is a process that maintains cell balance by degrading and reusing damaged organelles, proteins, invading pathogens and other substances. To some extent, autophagy can protect cells, while uncontrolled and defective autophagy will further aggravate cell damage. Currently, it has been reported that autophagy can reduce the levels of apoptosis and mesenchymal transformation caused by certain pathogenic factors. Methods: Western blot and Real-time PCR were used to detect the changes in EMT, and immunofluorescence and Western blot were employed to detect the levels of autophagy. Then, autophagy was inhibited, and the protein and nucleic acid levels of EMT marker were detected. Finally, the changes in the markers of the Transforming Growth Factor-β/Smad pathway were detected after the addition of the autophagy inhibitor 3-Methyladenine (3-MA). Results: After stimulating A549 cells with pyocyanin for 24 hours, the protein and messenger RNA (mRNA) expression levels of the epithelial marker E-cadherin were significantly decreased compared with those of the control group, while the protein and mRNA expression levels of the mesenchymal marker α-Smooth Muscle Actin (α-SMA) were increased compared with those of the control group (P < 0.05). After inhibiting autophagy with 3-MA, the protein and nucleic acid expression levels of the autophagy marker LC3 and the epithelial marker E-cadherin were significantly decreased compared with those of the control group, while the protein and nucleic acid expression levels of the mesenchymal marker α-SMA were increased compared with those of the control group (P < 0.05). Studies have shown that the nucleic acid level of Transforming Growth Factor-β1 and the protein expression level of phosphorylated Smad2/3 in the group with the addition of the autophagy inhibitor 3-MA were significantly increased compared with those of the control group and the pyocyanin group (P < 0.05). Conclusion: Pyocyanin can induce EMT and autophagy in alveolar epithelial cells, and autophagy can inhibit the further development of EMT. It may inhibit the occurrence of EMT by reducing the activity of the Transforming Growth Factor-β/Smad pathway. These results indicate that inhibition of autophagy may enhance EMT by affecting the Transforming Growth Factor-β/Smad pathway. Supplementary Material File (effect of autophagy on emt in alveolar epithelial cells induced by pyocyanin(1).docx) Download 347.61 KB Information & Authors Information Version history V1 Version 1 16 January 2025 Copyright This work is licensed under a Non Exclusive No Reuse License. Keyword other Authors Affiliations Li-zhuoyue [email protected] Southern Medical University Nanfang Hospital Department of Spinal Surgery View all articles by this author Wang-jiali Beijing Chaoyang Hospital Affiliated to Capital Medical University Department of Respiratory and Critical Care Medicine View all articles by this author Ye-huan Beijing Chaoyang Hospital Affiliated to Capital Medical University Department of Respiratory and Critical Care Medicine View all articles by this author Metrics & Citations Metrics Article Usage 150 views 65 downloads .FvxKWukQNSOunydq8rnd { width: 100px; } Citations Download citation Li-zhuoyue, Wang-jiali, Ye-huan. Effect of autophagy on pyocyanin-induced epithelial-mesenchymal transition (EMT) in alveolar epithelial cells. Authorea . 16 January 2025. DOI: https://doi.org/10.22541/au.173698923.36806423/v1 If you have the appropriate software installed, you can download article citation data to the citation manager of your choice. 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