Identification of Differentially Expressed mRNAs and lncRNAs in Uterine Leiomyomas with RNA Sequencing

Identification of Differentially Expressed mRNAs and lncRNAs in Uterine Leiomyomas with RNA Sequencing | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Identification of Differentially Expressed mRNAs and lncRNAs in Uterine Leiomyomas with RNA Sequencing Xinyu Chen, Fanfei Meng, Yijing Ji, Yuan Wang, Maofang Hua This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-1242687/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Objective : To explore the functions of mRNAs and lncRNAs in the occurrence of uterine leiomyomas (UL) and further clarify the pathogenesis of UL by detecting the differential expression of mRNAs and lncRNA in 10 cases of UL tissues and surrounding normal myometrial tissues by high-throughput RNA sequencing. Methods : The tissue samples of 10 patients who underwent hysterectomy for UL in Lianyungang maternal and child health hospital from January 2016 to December 2021 were collected. The differentially expressed mRNAs (DEmRNAs) and lncRNAs (DElncRNAs) were identified, and further analyzed by gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways enrichment analysis. The protein–protein interaction network (PPI) was constructed in Cytoscape software. Functional annotation of the nearby target cis‐DEmRNAs of DElncRNAs was performed with DAVID. Meanwhile, the co-expression network of DElncRNA-DEmRNA was constructed in Cytoscape software. Results : A total of 553 DElncRNAs (283 up-regulated DElncRNAs and 270 down-regulated DElncRNAs) and 3,293 DEmRNAs (1,632 up-regulated DEmRNAs and 1,661 down-regulated DEmRNAs) were obtained. GO pathways enrichment analysis revealed that several important pathways were significantly enriched in UL such as blood vessel development, regulation of ion transport and external encapsulating structure organization. In addition, Cytokine-cytokine receptor interaction, neuroactive ligand-receptor interaction and complement and coagulation cascades were significantly enriched in KEGG pathways enrichment analysis. A total of 409 DElncRNAs-nearby-targeted DEmRNA pairs were detected, which included 118 DElncRNAs and 136 DEmRNAs. Finally, we found that top two DElncRNAs with most nearby DEmRNAs were BISPR and AC012531.1. Conclusions : These results suggested that 3293 DEmRNAs and 553 DElncRNAs were differentially expressed in UL tissue and normal myometrium tissue, which might be candidate identified therapeutic and prognostic targets for UL and be considered as offering several possible mechanisms and pathogenesis of UL in the future. Uterine leiomyomas Long noncoding RNA mRNAs High-throughput-sequencing Figures Figure 1 Figure 2 Figure 3 Figure 4 Figure 5 Full Text Tables Table 1 Top 10 up-regulated and down-regulated DEmRNAs in uterine leiomyomas. Gene_ID Symbol log2FoldChange Pvalue Regulation ENSG00000142549 IGLON5 4.32 1.64E-15 up ENSG00000228203 RNF144A-AS1 2.13 1.23E-13 up ENSG00000153404 PLEKHG4B 4.23 7.86E-13 up ENSG00000183778 B3GALT5 3.38 2.14E-12 up ENSG00000260296 AC095057.3 1.85 8.65E-11 up ENSG00000235996 AL136090.1 3.52 1.09E-10 up ENSG00000140557 ST8SIA2 3.62 1.11E-10 up ENSG00000033122 LRRC7 2.78 2.71E-10 up ENSG00000275139 AL133492.1 2.65 4.14E-10 up ENSG00000157388 CACNA1D 2.20 5.00E-10 up ENSG00000127954 STEAP4 -3.57 5.72E-17 down ENSG00000137767 SQOR -2.23 4.60E-13 down ENSG00000158104 HPD -3.83 1.26E-12 down ENSG00000180914 OXTR -4.64 1.73E-11 down ENSG00000154198 CYP4Z2P -3.50 2.34E-11 down ENSG00000239265 CLRN1-AS1 -2.96 3.67E-11 down ENSG00000237510 GPAT2P1 -3.19 4.26E-11 down ENSG00000186160 CYP4Z1 -2.86 6.76E-11 down ENSG00000137699 TRIM29 -2.53 1.03E-10 down ENSG00000162591 MEGF6 -2.73 1.27E-10 down Count: number of DEGs that hit in the term Table 2 Top 10 up-regulated and down-regulated DElncRNAs in uterine leiomyomas. Gene_ID Symbol log2FoldChange Pvalue Regulation ENSG00000272002 AC010904.2 1.64 1.35E-09 up ENSG00000272371 AL591167.1 1.75 1.04E-08 up ENSG00000233723 LINC01122 4.00 1.95E-08 up ENSG00000259828 AL355596.1 4.37 1.87E-07 up ENSG00000226917 LINC01276 2.57 1.87E-07 up ENSG00000270147 AC068620.3 1.23 3.33E-07 up ENSG00000251536 AC055717.1 2.14 5.72E-07 up ENSG00000255628 AC140847.1 2.06 5.79E-07 up ENSG00000262772 LINC01977 3.58 6.03E-07 up ENSG00000243903 AC138057.1 2.16 6.05E-07 up ENSG00000246430 LINC00968 -2.69 8.95E-09 down ENSG00000230746 AC006007.1 -3.85 8.74E-07 down ENSG00000250742 LINC02381 -1.81 1.06E-06 down ENSG00000233117 LINC00702 -2.13 2.78E-06 down ENSG00000283897 AC011416.4 -2.61 3.04E-06 down ENSG00000272235 AL590438.1 -1.65 3.46E-06 down ENSG00000261462 AC004023.1 -1.83 4.39E-06 down ENSG00000277152 AC110048.2 -1.10 6.50E-06 down ENSG00000233521 LINC01638 -1.77 7.40E-06 down ENSG00000238033 AC002480.4 -1.89 9.57E-06 down Count: number of DEGs that hit in the term Additional Declarations No competing interests reported. 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Also discoverable on Platform About Our Team In Review Editorial Policies Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-1242687","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":76738742,"identity":"8d5835fa-251d-49fb-84be-bf461176171e","order_by":0,"name":"Xinyu Chen","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAAA1UlEQVRIiWNgGAWjYBACNvb+Dwc+/JCoZ5N/fIA4LXw8BwwfzuyxSeBnSEsgToucRIKxMQ9bWoJkQ44BkQ7jOZAmwcNzOM/gwJmPN94w2MnpNhDSwt5wTELC4nCxwcHezZZzGJKNzQ4QtOVgm4QBz2HGDYd5t0nzMBxI3EZQi0Qym0QCG1DLMZ5nxGpJYzY4wJaWOLOHh41ILTxnGB829tgY80uwGVvOMSDCL/LtPQyH//yQkGOTYH54402FnRxBLShAgofIqEHWQqqOUTAKRsEoGBEAAO9MQZaWqQJSAAAAAElFTkSuQmCC","orcid":"","institution":"Lianyungang maternal and child health hospital","correspondingAuthor":true,"submittingAuthor":false,"prefix":"","firstName":"Xinyu","middleName":"","lastName":"Chen","suffix":""},{"id":76738738,"identity":"2399534d-4635-43de-9b3e-c33c54fcd298","order_by":1,"name":"Fanfei Meng","email":"","orcid":"","institution":"Lianyungang maternal and child health hospital","correspondingAuthor":false,"submittingAuthor":false,"prefix":"","firstName":"Fanfei","middleName":"","lastName":"Meng","suffix":""},{"id":76738741,"identity":"b830397d-e5f7-4a61-b7b1-94ea11c28ec9","order_by":2,"name":"Yijing Ji","email":"","orcid":"","institution":"Lianyungang maternal and child health hospital","correspondingAuthor":false,"submittingAuthor":false,"prefix":"","firstName":"Yijing","middleName":"","lastName":"Ji","suffix":""},{"id":76738743,"identity":"a6cd2fa0-12ec-43ff-a431-f256d6618486","order_by":3,"name":"Yuan Wang","email":"","orcid":"","institution":"Lianyungang maternal and child health hospital","correspondingAuthor":false,"submittingAuthor":false,"prefix":"","firstName":"Yuan","middleName":"","lastName":"Wang","suffix":""},{"id":76738745,"identity":"efbfcb50-1446-439a-af98-b3f4ccf977ea","order_by":4,"name":"Maofang Hua","email":"","orcid":"","institution":"Lianyungang maternal and child health hospital","correspondingAuthor":false,"submittingAuthor":false,"prefix":"","firstName":"Maofang","middleName":"","lastName":"Hua","suffix":""}],"badges":[],"createdAt":"2022-01-09 07:59:05","currentVersionCode":1,"declarations":"","doi":"10.21203/rs.3.rs-1242687/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-1242687/v1","draftVersion":[],"editorialEvents":[],"editorialNote":"","failedWorkflow":false,"files":[{"id":17431168,"identity":"561cf591-e125-4715-9739-73749473886d","added_by":"auto","created_at":"2022-01-18 16:48:05","extension":"png","order_by":1,"title":"Figure 1","display":"","copyAsset":false,"role":"figure","size":1355369,"visible":true,"origin":"","legend":"\u003cp\u003eDElncRNAs and DEmRNAs expression patterns in uterine leiomyoma tissues relative to those in surrounding normal myometrial tissues. (A). Hierarchical cluster analysis of all differentially expressed DElncRNAs; (B). Hierarchical cluster analysis of all differentially expressed DEmRNAs; Each column represents a sample and each row represents a lncRNA and mRNA. The color scale indicates relative expression, upregulation (red), and downregulation (blue). A represents uterine leiomyoma tissue group and B represents surrounding normal myometrial tissue group; (C). Volcano plots and Scatter plots demonstrate differential expression of DElncRNAs between two different conditions; (D). Volcano plots and Scatter plots demonstrate differential expression of DEmRNAs between two different conditions; Red points indicated upregulated while the green points indicated downregulated expression. The values plotted on X and Y axes are the averaged normalized signal values of each group (log2 scaled). FC ≥ 2 and P ≤ 0.05 were regarded as the differentially expressed DElncRNAs\u0026nbsp;\u003c/p\u003e","description":"","filename":"Figure1.png","url":"https://assets-eu.researchsquare.com/files/rs-1242687/v1/d25a4436afaaffa4be068a09.png"},{"id":17431165,"identity":"000b202f-2fa5-4a4b-b78d-70a6629eee96","added_by":"auto","created_at":"2022-01-18 16:48:05","extension":"png","order_by":2,"title":"Figure 2","display":"","copyAsset":false,"role":"figure","size":851575,"visible":true,"origin":"","legend":"\u003cp\u003eFunctional annotation analysis for the validated DEmRNAs gathering genes. (A). GO analysis; (B). KEGG pathway analysis; The vertical axis shows the annotated functions of the target genes. The horizontal axes show the enrichment score (−log10 transformed p-value) and the gene number of each cluster, respectively. Only the top 10 significantly enriched clusters are included\u003c/p\u003e","description":"","filename":"Figure2.png","url":"https://assets-eu.researchsquare.com/files/rs-1242687/v1/ab1b5f6f41964ff06e106434.png"},{"id":17431520,"identity":"deb8f92d-da3c-4fa3-9ad0-7ad09fef58a8","added_by":"auto","created_at":"2022-01-18 16:51:05","extension":"png","order_by":3,"title":"Figure 3","display":"","copyAsset":false,"role":"figure","size":4995096,"visible":true,"origin":"","legend":"\u003cp\u003eProtein-protein interaction (PPI) networks analysis. The network comprising the top upregulated (red triangles) and downregulated circRNAs (green inverted arrows) and their target mRNAs are presented.\u003c/p\u003e","description":"","filename":"Figure3.png","url":"https://assets-eu.researchsquare.com/files/rs-1242687/v1/90dcaa6d9aeadbad7debedff.png"},{"id":17431519,"identity":"1a4a85ec-8b39-4a98-b56f-63aa22a1d8a7","added_by":"auto","created_at":"2022-01-18 16:51:05","extension":"png","order_by":4,"title":"Figure 4","display":"","copyAsset":false,"role":"figure","size":1999935,"visible":true,"origin":"","legend":"\u003cp\u003eCis-nearby-targeted DEmRNAs of DElncRNAs. There were 409 DElncRNAs-nearby-targeted DEmRNA pairs, including 118 DElncRNAs and 136 DEmRNAs.\u003c/p\u003e","description":"","filename":"Figure4.png","url":"https://assets-eu.researchsquare.com/files/rs-1242687/v1/81758d811a22c4b70f32ca24.png"},{"id":17431166,"identity":"ba62e4a2-a9e9-40be-bb6a-f68bd4f6e034","added_by":"auto","created_at":"2022-01-18 16:48:05","extension":"png","order_by":5,"title":"Figure 5","display":"","copyAsset":false,"role":"figure","size":573992,"visible":true,"origin":"","legend":"\u003cp\u003eFunctional annotation analysis for the validated cis-nearby-targeted DEmRNAs of DElncRNAs gathering genes. (A). GO analysis; (B). KEGG pathway analysis; The vertical axis shows the annotated functions of the target genes. The horizontal axes show the enrichment score (−log10 transformed p-value) and the gene number of each cluster, respectively. Only the top 10 significantly enriched clusters are included\u003c/p\u003e","description":"","filename":"Figure5.png","url":"https://assets-eu.researchsquare.com/files/rs-1242687/v1/f293a16d8df7c0cee0137b62.png"},{"id":17431521,"identity":"14d1a6ac-ea08-4b5a-98fc-bce137b97b70","added_by":"auto","created_at":"2022-01-18 16:51:12","extension":"pdf","order_by":7,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":268456,"visible":true,"origin":"","legend":"","description":"","filename":"Manuscript.pdf","url":"https://assets-eu.researchsquare.com/files/rs-1242687/v1_covered.pdf"}],"financialInterests":"No competing interests reported.","formattedTitle":"\u003cp\u003eIdentification of Differentially Expressed mRNAs and lncRNAs in Uterine Leiomyomas with RNA Sequencing\u003c/p\u003e","fulltext":[{"header":"Full Text","content":"This preprint is available for \u003ca href='/article/rs-1242687/latest.pdf' target='_blank'\u003edownload as a PDF\u003c/a\u003e."},{"header":"Tables","content":"\u003cdiv align=\"center\"\u003e\n \u003ctable border=\"0\" cellpadding=\"0\" cellspacing=\"0\" width=\"0\"\u003e\n \u003ctbody\u003e\n \u003ctr\u003e\n \u003ctd colspan=\"5\" valign=\"top\" width=\"100%\"\u003e\n \u003cp style=\"text-align: center;\"\u003e\u003cstrong\u003eTable 1\u003c/strong\u003e\u003c/p\u003e\n \u003cp style=\"text-align: center;\"\u003e\u0026nbsp;Top 10 up-regulated and down-regulated DEmRNAs in uterine leiomyomas.\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003e\n \u003cp\u003eGene_ID\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003eSymbol\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003elog2FoldChange\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003ePvalue\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003eRegulation\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003e\n 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\u003cp\u003eup\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003e\n \u003cp\u003eENSG00000235996\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003eAL136090.1\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e3.52\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e1.09E-10\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003eup\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003e\n \u003cp\u003eENSG00000140557\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003eST8SIA2\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e3.62\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e1.11E-10\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003eup\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003e\n \u003cp\u003eENSG00000033122\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n 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\u003cp\u003e5.00E-10\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003eup\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003e\n \u003cp\u003eENSG00000127954\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003eSTEAP4\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e-3.57\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e5.72E-17\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003edown\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003e\n \u003cp\u003eENSG00000137767\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003eSQOR\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e-2.23\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e4.60E-13\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003edown\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003e\n \u003cp\u003eENSG00000158104\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003eHPD\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e-3.83\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e1.26E-12\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003edown\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003e\n \u003cp\u003eENSG00000180914\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003eOXTR\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e-4.64\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e1.73E-11\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003edown\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003e\n \u003cp\u003eENSG00000154198\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003eCYP4Z2P\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e-3.50\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e2.34E-11\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003edown\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003e\n \u003cp\u003eENSG00000239265\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003eCLRN1-AS1\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e-2.96\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e3.67E-11\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003edown\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003e\n \u003cp\u003eENSG00000237510\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003eGPAT2P1\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e-3.19\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e4.26E-11\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003edown\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003e\n \u003cp\u003eENSG00000186160\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003eCYP4Z1\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e-2.86\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e6.76E-11\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003edown\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003e\n \u003cp\u003eENSG00000137699\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003eTRIM29\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e-2.53\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e1.03E-10\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003edown\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003e\n \u003cp\u003eENSG00000162591\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003eMEGF6\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e-2.73\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e1.27E-10\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003edown\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd colspan=\"5\"\u003e\n \u003cp\u003eCount: number of DEGs that hit in the term\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003c/tbody\u003e\n \u003c/table\u003e\n\u003c/div\u003e\n\u003cp\u003e\u003cbr\u003e\u003c/p\u003e\n\u003cdiv align=\"center\" id=\"isPasted\"\u003e\n \u003ctable border=\"0\" cellpadding=\"0\" cellspacing=\"0\" width=\"0\"\u003e\n \u003ctbody\u003e\n \u003ctr\u003e\n \u003ctd colspan=\"5\" width=\"100%\"\u003e\n \u003cp style=\"text-align: center;\"\u003e\u003cstrong\u003eTable 2\u003c/strong\u003e\u003c/p\u003e\n \u003cp style=\"text-align: center;\"\u003eTop 10 up-regulated and down-regulated DElncRNAs in uterine leiomyomas.\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003e\n \u003cp\u003eGene_ID\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003eSymbol\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003elog2FoldChange\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003ePvalue\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003eRegulation\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003e\n \u003cp\u003eENSG00000272002\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003eAC010904.2\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e1.64\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e1.35E-09\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003eup\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003e\n \u003cp\u003eENSG00000272371\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003eAL591167.1\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e1.75\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e1.04E-08\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003eup\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003e\n \u003cp\u003eENSG00000233723\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003eLINC01122\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e4.00\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e1.95E-08\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003eup\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003e\n \u003cp\u003eENSG00000259828\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003eAL355596.1\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e4.37\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e1.87E-07\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003eup\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003e\n \u003cp\u003eENSG00000226917\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003eLINC01276\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e2.57\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e1.87E-07\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003eup\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003e\n \u003cp\u003eENSG00000270147\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003eAC068620.3\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e1.23\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e3.33E-07\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003eup\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003e\n \u003cp\u003eENSG00000251536\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003eAC055717.1\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e2.14\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e5.72E-07\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003eup\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003e\n \u003cp\u003eENSG00000255628\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003eAC140847.1\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e2.06\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003e5.79E-07\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003eup\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003e\n \u003cp\u003eENSG00000262772\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n \u003cp\u003eLINC01977\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd\u003e\n 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\u003c/table\u003e\n\u003c/div\u003e"}],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":true,"highlight":"","institution":"","isAcceptedByJournal":false,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":false,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true},"keywords":"Uterine leiomyomas, Long noncoding RNA, mRNAs, High-throughput-sequencing","lastPublishedDoi":"10.21203/rs.3.rs-1242687/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-1242687/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003e\u003cstrong\u003eObjective\u003c/strong\u003e: To explore the functions of mRNAs and lncRNAs in the occurrence of uterine leiomyomas (UL) and further clarify the pathogenesis of UL by detecting the differential expression of mRNAs and lncRNA in 10 cases of UL tissues and surrounding normal myometrial tissues by high-throughput RNA sequencing. \u003c/p\u003e\u003cp\u003e\u003cstrong\u003eMethods\u003c/strong\u003e: The tissue samples of 10 patients who underwent hysterectomy for UL in Lianyungang maternal and child health hospital from January 2016 to December 2021 were collected. The differentially expressed mRNAs (DEmRNAs) and lncRNAs (DElncRNAs) were identified, and further analyzed by gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways enrichment analysis. The protein–protein interaction network (PPI) was constructed in Cytoscape software. Functional annotation of the nearby target cis‐DEmRNAs of DElncRNAs was performed with DAVID. Meanwhile, the co-expression network of DElncRNA-DEmRNA was constructed in Cytoscape software. \u003c/p\u003e\u003cp\u003e\u003cstrong\u003eResults\u003c/strong\u003e: A total of 553 DElncRNAs (283 up-regulated DElncRNAs and 270 down-regulated DElncRNAs) and 3,293 DEmRNAs (1,632 up-regulated DEmRNAs and 1,661 down-regulated DEmRNAs) were obtained. GO pathways enrichment analysis revealed that several important pathways were significantly enriched in UL such as blood vessel development, regulation of ion transport and external encapsulating structure organization. In addition, Cytokine-cytokine receptor interaction, neuroactive ligand-receptor interaction and complement and coagulation cascades were significantly enriched in KEGG pathways enrichment analysis. A total of 409 DElncRNAs-nearby-targeted DEmRNA pairs were detected, which included 118 DElncRNAs and 136 DEmRNAs. Finally, we found that top two DElncRNAs with most nearby DEmRNAs were BISPR and AC012531.1. \u003c/p\u003e\u003cp\u003e\u003cstrong\u003eConclusions\u003c/strong\u003e: These results suggested that 3293 DEmRNAs and 553 DElncRNAs were differentially expressed in UL tissue and normal myometrium tissue, which might be candidate identified therapeutic and prognostic targets for UL and be considered as offering several possible mechanisms and pathogenesis of UL in the future.\u003c/p\u003e","manuscriptTitle":"Identification of Differentially Expressed mRNAs and lncRNAs in Uterine Leiomyomas with RNA Sequencing","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2022-01-18 16:48:03","doi":"10.21203/rs.3.rs-1242687/v1","editorialEvents":[{"type":"communityComments","content":0}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"4ba2f9ed-ec8a-4ca7-9249-6eff05a4656e","owner":[],"postedDate":"January 18th, 2022","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"posted","subjectAreas":[],"tags":[],"updatedAt":"2023-02-17T12:44:33+00:00","versionOfRecord":[],"versionCreatedAt":"2022-01-18 16:48:03","video":"","vorDoi":"","vorDoiUrl":"","workflowStages":[]},"version":"v1","identity":"rs-1242687","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-1242687","identity":"rs-1242687","version":["v1"]},"buildId":"omnImTCwR2MFx8CMYfrG7","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}