MicroRNA 125b overexpression in pseudoexfoliation syndrome

Abstract Background MicroRNAs (miRs) are small non-coding RNAs. Qin et al. described miR 125b was downregulated in cataract tissue when compared to a transparent lens. The aim of study was (1) to establish the expression of miR 125b in cataracts complicated by pseudoexfoliation syndrome /PEX/, glaucoma or pseudoexfoliation glaucoma and (2) to determine whether any environmental factors influence miR 125b expression. Methods Anterior lens capsule were obtained from 150 patients. The patients were selected for three groups: with PEX, primary open angle glaucoma, pseudoexfoliation glaucoma, with matched controls in the term of gender (cataract alone). qPCR expression of microRNA 125b was examined in every groups. Results Mean age of 150 patients was 75.18years (SD +/-9.12years). Investigation indicates for the first time, that miR 125b expression is significantly increased 3.33x in PEX group (p=0,046). A lower level of miR 125b (p=0,01) was found in patients who smoked compared to those who did not. Conclusions Our data reveal the possible role of miR 125b in pseudoexfoliation syndrome development. There are two possible interpretations of these results. Either the co-existence of PEX acts as a moderator of miR 125b expression in the anterior lens, or the increased expression of miR 125b can be a cause of PEX.