Impaired Brain Development in ALG13 Knockout Mice: Behavioral and Molecular Evidence

Impaired Brain Development in ALG13 Knockout Mice: Behavioral and Molecular Evidence | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Impaired Brain Development in ALG13 Knockout Mice: Behavioral and Molecular Evidence Baorui Guo, Xiuhua Li, Yangyang Sun, Peng Gao, Zhuoqi Li, Chunlin Wang, and 8 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-5233818/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Asparagine-linked glycosylation (ALG) 13 is an important participant in protein N-terminal glycosylation. Children with ALG13-congenital glycosylation disorders caused by ALG13 mutations usually have neurological developmental disorders. However, the current body of research on the correlation between ALG13 and brain development is limited. This study conducted a series of behavioral tests on ALG13 knock out (KO) and wild type mice from birth to adulthood, and found that the neurological development of ALG13KO mice was slow than wild type. Immunofluorescence staining of the cerebral cortex showed abnormal distribution of cortical specific localization protein forkhead box protein 2 and coup -TF- Interacting protein 2 in the cerebral cortex of ALG13KO mice. The proteomic, protein-protein interaction, and parallel reaction monitoring results revealed a significant number of differentially expressed proteins in the cerebral cortex of ALG13KO mice one week after birth. Notably, these proteins exhibited a strong correlation with the PI3K/AKT/mTOR pathway. Further Western blot validation showed that the activation of PI3K/AKT/mTOR was inhibited. This suggest that the activation of the PI3K/AKT/mTOR pathway is inhibited after ALG13KO, which affects brain development. ALG13 brain development congenital glycosylation disorders N-terminal glycosylation neuronal distribution Full Text Additional Declarations No competing interests reported. Supplementary Files ESM1.tif ESM2.docx Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-5233818","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":369105161,"identity":"3cd5877a-52ca-4f08-b29d-9c6c3fc3c9cb","order_by":0,"name":"Baorui Guo","email":"","orcid":"","institution":"Department of Neurosurgery, the First Medical Center of Chinese PLA General Hospital","correspondingAuthor":false,"prefix":"","firstName":"Baorui","middleName":"","lastName":"Guo","suffix":""},{"id":369105162,"identity":"119719a9-89f6-4198-8bc2-7519e1c4e898","order_by":1,"name":"Xiuhua Li","email":"","orcid":"","institution":"Department of 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