Signification of 8-iso-PGF2α and GSH-Px in blood serum and peritoneal fluid of patients with endometriosis and infertility

Objective:To study the expression of 8-iso-PGF2αand GSH-Px in blood serum and peritoneal fluid of endometriosis and infertility patients.Methods:The levels of 8-iso-PGF2α and GSH-Px in blood serum and peritoneal fluid(PF) were measured in 50 patients with endometriosis and infertility and 30 control patients.The group of endometriosis and infertility included group A(endometriosis stage Ⅰ,Ⅱ) and group B (endometriosis stage Ⅲ,Ⅳ).The level of 8-iso-PGF2α were measured by enzyme-linked immunosorbent assay (ELISA) and GSH-Px by the chromatometry.Results:①The concentration of 8-iso-PGF2αin blood serum and PF with endometriosis and infertility group were(210.656±21.013)and(42.23+4.316)ng/ml.The concentration of GSH-Px in blood serum and peritoneal fluid with endometriosis and infertility group were(251.076±21.1)and(154.843+30.224)U.The concentration of 8-iso-PGF2αwere statistically higher and GSH-Px were statistically lower in endometriosis and infertility group (P0.01).②The levels of 8-iso-PGF2αin blood serum [(220.496±18.201)ng/ml] and PF with group B [(45.257±2.84)ng/ml] were significantly increased (P0.01).The levels of GSH-Px in blood serum [(240.382±16.977)U] and PF [(137.543±26.36)U] with group B(endometriosis stage Ⅲ,Ⅳ) were significantly dropped (P0.01).Conclusion:①8-iso-PGF2α and GSH-PX are associated in the pathophysiology of endometriosis and infertility. ②8-iso-PGF2a is a reliable, sensitive and a specific biomarker of oxidative stress of endometriosis and infertility.