Response of Elymus nutans seedling physiology and endogenous hormones to drought and salt stress

Response of Elymus nutans seedling physiology and endogenous hormones to drought and salt stress | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Response of Elymus nutans seedling physiology and endogenous hormones to drought and salt stress Jianting Long, Dandan Liu, Wei Qiao, Yanwei Wang, Yanjun Miao, and 1 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-3352763/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Elymus nutans, a pioneer plant for the restoration of high quality pasture and vegetation, is widely used to establish artificial grasslands and ecologically restore arid and salinized soils. To investigate the effects of drought stress and salt stress on the physiology and endogenous hormones of E. nutans seedlings, this experiment configured the same environmental water potential of PEG-6000 and NaCl stress to investigate the effects of drought stress and salt stress, respectively, on E. nutans seedlings under the same environmental water potential. The results showed that although the physiological indices and endogenous hormones of the E. nutans seedlings responded differently to drought stress and salt stress under the same environmental water potential, the physiological indices of E. nutans leaves and roots were comprehensively evaluated using the genus function method, and the physiological indices of the E. nutans seedlings under the same environmental water potential exhibited better salt tolerance than drought tolerance. The changes in endogenous hormones of the E. nutans seedlings under drought stress were analyzed to find that treatment with gibberellic acid (GA3), gibberellin A7 (GA7), 6-benzyladenine (6-BA), 6-(y,y-dimethylallylaminopurine) (2.IP), trans-zeatin (TZ), kinetin (KT), dihydrozeatin (DHZ), indole acetic acid (IAA), and 2,6-dichloroisonicotininc acid (INA) was more effective than those under drought stress. By analyzing the amplitude of changes in the endogenous hormones in E. nutans seedlings, the amplitude of changes in the contents of GA3, GA7, 6-BA, 2.IP, TZ, KT, DHZ, IAA, isopentenyl adenosine (IPA), indole-3-butyric acid (IBA), naphthalene acetic acid (NAA), and abscisic acid was larger in drought stress compared with salt stress, which could be because the endogenous hormones are important for the drought tolerance of E. nutans itself. The amplitude of the changes in the contents of DHZ, TZR, salicylic acid, and jasmonic acid was larger in salt stress compared with salt stress. Changes in the content of melatonin were larger in salt stress compared with drought stress, which could indicate that endogenous hormones and substances are important for the salt tolerance of E. nutans itself. Elymus nutans drought stress salt stress seedling physiology endogenous hormones Figures Figure 1 Figure 2 Figure 3 Figure 4 Figure 5 Figure 6 Figure 7 Figure 8 Full Text Additional Declarations Table 1 and 2 are available in the Supplementary Files section. Supplementary Files Table1.docx Table2.docx Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-3352763","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":234181244,"identity":"5bdd2ac8-a1c2-4fce-8edf-9fe2293ca553","order_by":0,"name":"Jianting Long","email":"","orcid":"","institution":"Tibet Agricultural and Animal Husbandry University","correspondingAuthor":false,"submittingAuthor":false,"prefix":"","firstName":"Jianting","middleName":"","lastName":"Long","suffix":""},{"id":234181245,"identity":"5ae5a81d-9a5b-436e-972a-c2c90d3c3c4b","order_by":1,"name":"Dandan Liu","email":"","orcid":"","institution":"Tibet Agricultural and Animal Husbandry University","correspondingAuthor":false,"submittingAuthor":false,"prefix":"","firstName":"Dandan","middleName":"","lastName":"Liu","suffix":""},{"id":234181246,"identity":"496e2843-e998-4c1f-bac2-fb246558353e","order_by":2,"name":"Wei Qiao","email":"","orcid":"","institution":"Tibet Agricultural and Animal Husbandry University","correspondingAuthor":false,"submittingAuthor":false,"prefix":"","firstName":"Wei","middleName":"","lastName":"Qiao","suffix":""},{"id":234181247,"identity":"e8273d2b-074a-43e8-8741-b47c745962ab","order_by":3,"name":"Yanwei Wang","email":"","orcid":"","institution":"Tibet Agricultural and Animal Husbandry University","correspondingAuthor":false,"submittingAuthor":false,"prefix":"","firstName":"Yanwei","middleName":"","lastName":"Wang","suffix":""},{"id":234181248,"identity":"ba7ca52e-a3bf-4f46-9fc2-165d1f83387b","order_by":4,"name":"Yanjun Miao","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAAAr0lEQVRIiWNgGAWjYHCCxAcfKmx4+NkbiNeSbDjjTJqMZM8B4rWwCXO2HLYxuOFApHrdGQnPmBkbzvMw3GBg/PAxhwgtZjcS0h4X7rjNwzi7gVly5jbitKQbzzxzm4dZ5gAbMy+RWtKkedvO8bBJJJCm5QAPD/FazjwABXIyjwTPwWYi/XI8BxSVdvb2x5sPfvhIjBYGgZwEKIuxgRj1QMB//ACRKkfBKBgFo2DEAgBl8Trx/28E5wAAAABJRU5ErkJggg==","orcid":"","institution":"Tibet Agricultural and Animal Husbandry University","correspondingAuthor":true,"submittingAuthor":false,"prefix":"","firstName":"Yanjun","middleName":"","lastName":"Miao","suffix":""},{"id":234181249,"identity":"36520e0e-3bc0-46ce-95df-e723707883a6","order_by":5,"name":"Henna Baosai","email":"","orcid":"","institution":"Tibet Agricultural and Animal Husbandry University","correspondingAuthor":false,"submittingAuthor":false,"prefix":"","firstName":"Henna","middleName":"","lastName":"Baosai","suffix":""}],"badges":[],"createdAt":"2023-09-13 16:14:20","currentVersionCode":1,"declarations":"","doi":"10.21203/rs.3.rs-3352763/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-3352763/v1","draftVersion":[],"editorialEvents":[],"editorialNote":"","failedWorkflow":false,"files":[{"id":43644936,"identity":"a0aeedf4-73ec-49c7-ae8c-9d4749b29bb8","added_by":"auto","created_at":"2023-09-25 16:07:10","extension":"png","order_by":1,"title":"Figure 1","display":"","copyAsset":false,"role":"figure","size":367386,"visible":true,"origin":"","legend":"\u003cp\u003eEffects of two solutions on biomass accumulation. Two types of solution stress affect \u003cem\u003eE. nutans\u003c/em\u003e Leaf fresh weight (A), leaf dry weight (B), Relative leaf water content (C), Root fresh weight(D), root dry weight(E), and Relative water content of the root system(F). Different lowercase letters indicate significant differences in the same solution under different treatments (\u003cem\u003eP\u003c/em\u003e \u0026lt; 0.05), different capital letters indicate that the differences in the same solution under different treatments reach a highly significant level (\u003cem\u003eP\u003c/em\u003e \u0026lt; 0.01).\u003c/p\u003e","description":"","filename":"Figure1.png","url":"https://assets-eu.researchsquare.com/files/rs-3352763/v1/309760f70460963d28f45528.png"},{"id":43644171,"identity":"b5fe94c0-81da-4363-a318-8f8fac50ca48","added_by":"auto","created_at":"2023-09-25 15:51:11","extension":"png","order_by":2,"title":"Figure 2","display":"","copyAsset":false,"role":"figure","size":99741,"visible":true,"origin":"","legend":"\u003cp\u003eEffects of two solutions on Leaf Physiological Indicators. Two types of solution stress affect \u003cem\u003eE. nutans\u003c/em\u003e Chlorophyll a content(A),Chlorophyll b content(B), leaf POD activated(C), leaf SOD activated(D), leaf Pro content(E), leaf SP content(F), and leaf MDA content(G). Different lowercase letters indicate significant differences in the same solution under different treatments (\u003cem\u003eP\u003c/em\u003e \u0026lt; 0.05), different capital letters indicate that the differences in the same solution under different treatments reach a highly significant level (\u003cem\u003eP\u003c/em\u003e \u0026lt; 0.01).\u003c/p\u003e","description":"","filename":"Figure2.png","url":"https://assets-eu.researchsquare.com/files/rs-3352763/v1/26e13ebe97052b67f8231a56.png"},{"id":43644169,"identity":"bd861e08-88c4-4334-8073-fb50ae5ec980","added_by":"auto","created_at":"2023-09-25 15:51:11","extension":"png","order_by":3,"title":"Figure 3","display":"","copyAsset":false,"role":"figure","size":273537,"visible":true,"origin":"","legend":"\u003cp\u003eEffects of two solutions on Leaf Physiological Indicators. Two types of solution stress affect \u003cem\u003eE. nutans\u003c/em\u003e root POD activated(A),root SOD activated(B), root SP content(C), root MDA content(D)and leaf Pro content(E). Different lowercase letters indicate significant differences in the same solution under different treatments (\u003cem\u003eP\u003c/em\u003e \u0026lt; 0.05), different capital letters indicate that the differences in the same solution under different treatments reach a highly significant level (\u003cem\u003eP\u003c/em\u003e\u0026lt; 0.01).\u003c/p\u003e","description":"","filename":"Figure3.png","url":"https://assets-eu.researchsquare.com/files/rs-3352763/v1/2349698a77f829dae8342343.png"},{"id":43644512,"identity":"bb69f7ce-6776-4005-b536-1d0f6f120274","added_by":"auto","created_at":"2023-09-25 15:59:10","extension":"png","order_by":4,"title":"Figure 4","display":"","copyAsset":false,"role":"figure","size":288284,"visible":true,"origin":"","legend":"\u003cp\u003eEffects of two solutions on GA content. Two types of solution stress affect \u003cem\u003eE. nutans\u003c/em\u003e GA\u003csub\u003e1\u003c/sub\u003e content(A),GA\u003csub\u003e3\u003c/sub\u003e content(B), GA\u003csub\u003e4\u003c/sub\u003e content(C)and GA\u003csub\u003e7\u003c/sub\u003e content(D). Different lowercase letters indicate significant differences in the same solution under different treatments (P \u0026lt; 0.05), different capital letters indicate that the differences in the same solution under different treatments reach a highly significant level (P \u0026lt; 0.01).\u003c/p\u003e","description":"","filename":"Figure4.png","url":"https://assets-eu.researchsquare.com/files/rs-3352763/v1/48d6984a9952748ba1add7d6.png"},{"id":43644172,"identity":"3a0fff44-d8f5-4b7b-85bd-89cdfcf421bc","added_by":"auto","created_at":"2023-09-25 15:51:11","extension":"png","order_by":5,"title":"Figure 5","display":"","copyAsset":false,"role":"figure","size":274469,"visible":true,"origin":"","legend":"\u003cp\u003eEffects of two solutions on auxin content. Two types of solution stress affect \u003cem\u003eE. nutans\u003c/em\u003e IAA content(A),IPA content(B), IBA content(C)and 2ip-riboside content(D) and NAA content(E). Different lowercase letters indicate significant differences in the same solution under different treatments (\u003cem\u003eP\u003c/em\u003e \u0026lt; 0.05), different capital letters indicate that the differences in the same solution under different treatments reach a highly significant level (\u003cem\u003eP\u003c/em\u003e\u0026lt; 0.01).\u003c/p\u003e","description":"","filename":"Figure5.png","url":"https://assets-eu.researchsquare.com/files/rs-3352763/v1/99378376c5b2bb9eaa6c90a3.png"},{"id":43644514,"identity":"dd6995b6-b9fc-4a92-bfd0-871792b59f2a","added_by":"auto","created_at":"2023-09-25 15:59:11","extension":"png","order_by":6,"title":"Figure 6","display":"","copyAsset":false,"role":"figure","size":299148,"visible":true,"origin":"","legend":"\u003cp\u003eEffects of two solutions on cytokinin content. Two types of solution stress affect \u003cem\u003eE. nutans\u003c/em\u003e 6-BA content(A),2.IP content(B), KT content(C),DHZ content(D) , TZ content(E) and TZR content(F). Different lowercase letters indicate significant differences in the same solution under different treatments (\u003cem\u003eP\u003c/em\u003e \u0026lt; 0.05), different capital letters indicate that the differences in the same solution under different treatments reach a highly significant level (\u003cem\u003eP\u003c/em\u003e \u0026lt; 0.01).\u003c/p\u003e","description":"","filename":"Figure6.png","url":"https://assets-eu.researchsquare.com/files/rs-3352763/v1/146183c7cdff7a33e406b90b.png"},{"id":43644168,"identity":"7f78a48b-0469-4358-b2ff-5814b540686d","added_by":"auto","created_at":"2023-09-25 15:51:10","extension":"png","order_by":7,"title":"Figure 7","display":"","copyAsset":false,"role":"figure","size":316654,"visible":true,"origin":"","legend":"\u003cp\u003eEffects of two solutions on Abscisic acid and other substances content. Two types of solution stress affect E. nutans ABA content(A),SA content(B), JA content(C)and MT content(D). Different lowercase letters indicate significant differences in the same solution under different treatments (P \u0026lt; 0.05), different capital letters indicate that the differences in the same solution under different treatments reach a highly significant level (P \u0026lt; 0.01).\u003c/p\u003e","description":"","filename":"Figure7.png","url":"https://assets-eu.researchsquare.com/files/rs-3352763/v1/78ac727a110542ed4102b4d3.png"},{"id":43644516,"identity":"d44d768c-7f06-4e59-915b-462ed6cb8da8","added_by":"auto","created_at":"2023-09-25 15:59:11","extension":"png","order_by":8,"title":"Figure 8","display":"","copyAsset":false,"role":"figure","size":7135558,"visible":true,"origin":"","legend":"\u003cp\u003eComprehensive analysis. principal component analysis of PEG-6000 on Physiological indicators (A); principal component analysis of NaCl on Physiological indicators (B); Comprehensive analysis.Mantel test analysis of PEG-6000 on Physiological indicators andHormonal indicators (C); Mantel test analysis of NaCl on Physiological indicators and Hormonal indicators(D);Comprehensive analysis.Structural equation modeling (SEM) analysis of PEG-6000 on Interaction between endogenous hormones and leaf and root biomass accumulation (E); Structural equation modeling (SEM) analysis of NaCl on Interaction between endogenous hormones and leaf and root biomass accumulation(F)\u003c/p\u003e","description":"","filename":"Figure8.png","url":"https://assets-eu.researchsquare.com/files/rs-3352763/v1/6a869d9f6f80239f45b118cb.png"},{"id":43935477,"identity":"02a6ac57-e06d-4df5-a700-6693ad00ab96","added_by":"auto","created_at":"2023-09-30 22:22:30","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":912666,"visible":true,"origin":"","legend":"","description":"","filename":"Manuscript.pdf","url":"https://assets-eu.researchsquare.com/files/rs-3352763/v1_covered_82f5a9fd-8626-4e2b-8331-5be5fd125503.pdf"},{"id":43644164,"identity":"035fa8d2-2443-4a65-8eda-892a430ed136","added_by":"auto","created_at":"2023-09-25 15:51:10","extension":"docx","order_by":1,"title":"","display":"","copyAsset":false,"role":"supplement","size":17241,"visible":true,"origin":"","legend":"","description":"","filename":"Table1.docx","url":"https://assets-eu.researchsquare.com/files/rs-3352763/v1/17a960b32d50e6a22c46216b.docx"},{"id":43644165,"identity":"2f800210-b42d-49df-8188-d51f5ccd1dfa","added_by":"auto","created_at":"2023-09-25 15:51:10","extension":"docx","order_by":2,"title":"","display":"","copyAsset":false,"role":"supplement","size":17192,"visible":true,"origin":"","legend":"","description":"","filename":"Table2.docx","url":"https://assets-eu.researchsquare.com/files/rs-3352763/v1/fa8a9ae3a09b16f14fa71065.docx"}],"financialInterests":"\u003cp\u003eTable 1 and 2 are available in the Supplementary Files section.\u003c/p\u003e","formattedTitle":"Response of Elymus nutans seedling physiology and endogenous hormones to drought and salt stress","fulltext":[],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":true,"highlight":"","institution":"","isAcceptedByJournal":false,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":true,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true},"keywords":"Elymus nutans, drought stress, salt stress, seedling physiology, endogenous hormones","lastPublishedDoi":"10.21203/rs.3.rs-3352763/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-3352763/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"Elymus nutans, a pioneer plant for the restoration of high quality pasture and vegetation, is widely used to establish artificial grasslands and ecologically restore arid and salinized soils. To investigate the effects of drought stress and salt stress on the physiology and endogenous hormones of E. nutans seedlings, this experiment configured the same environmental water potential of PEG-6000 and NaCl stress to investigate the effects of drought stress and salt stress, respectively, on E. nutans seedlings under the same environmental water potential. The results showed that although the physiological indices and endogenous hormones of the E. nutans seedlings responded differently to drought stress and salt stress under the same environmental water potential, the physiological indices of E. nutans leaves and roots were comprehensively evaluated using the genus function method, and the physiological indices of the E. nutans seedlings under the same environmental water potential exhibited better salt tolerance than drought tolerance. The changes in endogenous hormones of the E. nutans seedlings under drought stress were analyzed to find that treatment with gibberellic acid (GA3), gibberellin A7 (GA7), 6-benzyladenine (6-BA), 6-(y,y-dimethylallylaminopurine) (2.IP), trans-zeatin (TZ), kinetin (KT), dihydrozeatin (DHZ), indole acetic acid (IAA), and 2,6-dichloroisonicotininc acid (INA) was more effective than those under drought stress. By analyzing the amplitude of changes in the endogenous hormones in E. nutans seedlings, the amplitude of changes in the contents of GA3, GA7, 6-BA, 2.IP, TZ, KT, DHZ, IAA, isopentenyl adenosine (IPA), indole-3-butyric acid (IBA), naphthalene acetic acid (NAA), and abscisic acid was larger in drought stress compared with salt stress, which could be because the endogenous hormones are important for the drought tolerance of E. nutans itself. The amplitude of the changes in the contents of DHZ, TZR, salicylic acid, and jasmonic acid was larger in salt stress compared with salt stress. Changes in the content of melatonin were larger in salt stress compared with drought stress, which could indicate that endogenous hormones and substances are important for the salt tolerance of E. nutans itself.","manuscriptTitle":"Response of Elymus nutans seedling physiology and endogenous hormones to drought and salt stress","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2023-09-25 15:51:06","doi":"10.21203/rs.3.rs-3352763/v1","editorialEvents":[{"type":"communityComments","content":0}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"6e45393a-7394-4a94-ba7d-8ccbdf38610e","owner":[],"postedDate":"September 25th, 2023","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"posted","subjectAreas":[],"tags":[],"updatedAt":"2023-09-30T22:14:12+00:00","versionOfRecord":[],"versionCreatedAt":"2023-09-25 15:51:06","video":"","vorDoi":"","vorDoiUrl":"","workflowStages":[]},"version":"v1","identity":"rs-3352763","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-3352763","identity":"rs-3352763","version":["v1"]},"buildId":"cBFmMYwuxLRRLfASyISRj","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}