Optimizing Melatonin Pulse Treatment for Enhanced Postharvest Vase Life and Quality in Cut Roses (Rosa hybrida cv. Samurai)

Optimizing Melatonin Pulse Treatment for Enhanced Postharvest Vase Life and Quality in Cut Roses (Rosa hybrida cv. Samurai) | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Optimizing Melatonin Pulse Treatment for Enhanced Postharvest Vase Life and Quality in Cut Roses (Rosa hybrida cv. Samurai) Sara Shamsinejad, Vahid Reza Saffari¹, Safoora Saadati¹, Zahra Pakkish¹ This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-7751050/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Optimizing Melatonin Pulse Treatment for Enhanced Postharvest Vase Life and Quality in Cut Roses ( Rosa hybrida cv. Samurai) Background Cut roses ( Rosa hybrida L. cv. Samurai) undergo rapid postharvest senescence, limiting vase life and quality. This study evaluated melatonin's efficacy as a biostimulant, applied via pulse (30-min immersion) or continuous (vase solution) methods at 0.1 mM and 1 mM concentrations, in a completely randomized design with three replicates of 10 stems per treatment. Assessed parameters included vase life, flower quality, diameter, chlorophyll and anthocyanin contents, electrolyte leakage, relative water content, solution uptake, and antioxidant enzyme activities. Results The 0.1 mM pulse treatment extended vase life by 61% (9.67 vs. 6.00 days), improved flower quality (5.00 vs. 3.33), increased diameter by 18% (51.00 vs. 43.33 mm), enhanced chlorophyll by 22% and anthocyanin by 36%, and reduced electrolyte leakage by 43%, with correlations to vase life (r = 0.71–0.94, P < 0.001). The 0.1 mM continuous treatment was less effective, and 1 mM concentrations showed minimal benefits. Conclusion Melatonin's antioxidant and anti-senescence properties provide a sustainable, cost-effective strategy for extending rose vase life, with 0.1 mM pulse application as the most effective method for commercial floriculture. Melatonin Postharvest Roses Vase life Quality Full Text Additional Declarations No competing interests reported. Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-7751050","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":541058683,"identity":"6d23c9ac-54ba-451e-bbff-54b164d28cff","order_by":0,"name":"Sara Shamsinejad","email":"","orcid":"","institution":"Shahid Bahonar University of Kerman","correspondingAuthor":false,"prefix":"","firstName":"Sara","middleName":"","lastName":"Shamsinejad","suffix":""},{"id":541058684,"identity":"32f91332-c7c9-4223-88d4-3c12257e8f43","order_by":1,"name":"Vahid Reza Saffari¹","email":"","orcid":"","institution":"Shahid Bahonar University of 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Samurai)","fulltext":[],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":true,"highlight":"","institution":"","isAcceptedByJournal":false,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":true,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true},"keywords":"Melatonin, Postharvest, Roses, Vase life, Quality","lastPublishedDoi":"10.21203/rs.3.rs-7751050/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-7751050/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cdiv class=\"Heading\"\u003eOptimizing Melatonin Pulse Treatment for Enhanced Postharvest Vase Life and Quality in Cut Roses (\u003cem\u003eRosa hybrida\u003c/em\u003e cv. Samurai)\u003c/div\u003e\u003cp\u003e\u003cb\u003eBackground\u003c/b\u003e\u003c/p\u003e\u003cp\u003eCut roses (\u003cem\u003eRosa hybrida\u003c/em\u003e L. cv. Samurai) undergo rapid postharvest senescence, limiting vase life and quality. This study evaluated melatonin's efficacy as a biostimulant, applied via pulse (30-min immersion) or continuous (vase solution) methods at 0.1 mM and 1 mM concentrations, in a completely randomized design with three replicates of 10 stems per treatment. Assessed parameters included vase life, flower quality, diameter, chlorophyll and anthocyanin contents, electrolyte leakage, relative water content, solution uptake, and antioxidant enzyme activities.\u003c/p\u003e\u003cp\u003e\u003cb\u003eResults\u003c/b\u003e\u003c/p\u003e\u003cp\u003eThe 0.1 mM pulse treatment extended vase life by 61% (9.67 vs. 6.00 days), improved flower quality (5.00 vs. 3.33), increased diameter by 18% (51.00 vs. 43.33 mm), enhanced chlorophyll by 22% and anthocyanin by 36%, and reduced electrolyte leakage by 43%, with correlations to vase life (r\u0026thinsp;=\u0026thinsp;0.71\u0026ndash;0.94, P\u0026thinsp;\u0026lt;\u0026thinsp;0.001). The 0.1 mM continuous treatment was less effective, and 1 mM concentrations showed minimal benefits.\u003c/p\u003e\u003cp\u003e\u003cb\u003eConclusion\u003c/b\u003e\u003c/p\u003e\u003cp\u003eMelatonin's antioxidant and anti-senescence properties provide a sustainable, cost-effective strategy for extending rose vase life, with 0.1 mM pulse application as the most effective method for commercial floriculture.\u003c/p\u003e","manuscriptTitle":"Optimizing Melatonin Pulse Treatment for Enhanced Postharvest Vase Life and Quality in Cut Roses (Rosa hybrida cv. 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