Additional file 1 of Activation of α7 nicotinic acetylcholine receptor retards the development of endometriosis

Additional file 1: SupplementaryFigure S1. Positive andnegative controls for immunohistochemistry. For positive controls, human breastcancer tissues were used for E-cadherin, mouse liver tissues for α-SMA, humanadenomyotic tissue samples for desmin and SM-MHC, and mouse lung tissues for α7nAChR.For negative controls, mouse endometriotic lesions were used. Magnification: 400×;Scale bar: 50 μm. Supplementary Figure S2. Cysticappearance of endometriotic lesions in mouse experiment 1. Lesions seen fromExperiment 2 are similar. Supplementary Figure S3. The number ofendometriotic lesions per mouse was evaluated from the Control, PNU-282987 andMLA groups in mouse experiment 1. Symbols forstatistical significance levels: NS: p>0.05; *: p<0.05;**: p<0.01. SupplementaryFigure S4. H&E staining from endometriotic lesions. Magnification = 200×, scale bar=100 μm. Supplementary Figure S5. The number of endometrioticlesions per mouse was evaluated from the Control and PNU-282987 groups in mouse experiment 2. NS: p>0.05.