Unraveling the Self-Assembly Mechanisms in Bacterial Cellulose Hydrogels

Abstract Bacterial cellulose (BC) hydrogels produced by Gluconacetobacter species hold considerable promise for a wide range of applications owing to their exceptional mechanical properties, biocompatibility, and biodegradability. Achieving precise control over their structural and mechanical characteristics is crucial for the engineering of BC-based materials. In this study, we investigated the formation dynamics and structural features of BC hydrogels, emphasizing the complex interplay between cellulose nanofibril secretion and bacterial motility. Comprehensive tracking of bacterial movement during hydrogel formation has validated mechanisms underlying the development of branching and merging junctions, which are key elements that define the network’s physical properties. Additionally, we observed the emergence of vortex-lattice and chiral-nematic structures during hydrogel development, depending on bacterial and cellulose densities. These insights contribute to a fundamental understanding of bottom-up 3D fabrication of BC hydrogels that harness the collective behavior of cellulose-producing bacteria. Competing Interest Statement The authors have declared no competing interest. Footnotes gotakayama{at}noneq.phys.s.u-tokyo.ac.jp (G. Takayama)