Revealing the Immune Heterogeneity in Systemic Lupus Erythematosus Based on Multi-Omics Data Analysis

Revealing the Immune Heterogeneity in Systemic Lupus Erythematosus Based on Multi-Omics Data Analysis | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Revealing the Immune Heterogeneity in Systemic Lupus Erythematosus Based on Multi-Omics Data Analysis Hao Liu, Yadong Gong, Mubo Liu, Ran Xiao, Ma Qingqing This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-3814377/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Systemic lupus erythematosus is an autoimmune disorder characterized by a spectrum of clinical manifestations. With the progress of next-generation sequencing (NGS) technology, novel techniques for sequencing T cell receptors and B cell receptors have emerged. In this study, we employed the computational approach TRUST4 to construct TCR and BCR libraries using a substantial volume of RNA-seq data extracted from the peripheral blood of sepsis patients. Subsequently, we conducted an analysis to assess the clonality and diversity of the immune repertoire associated with this disease. A total of 30 distinct cell types were annotated and subsequently categorized into 12 clusters. SLE group demonstrated an increase in the innate immune responses of CD14 monocytes, CD16 monocytes, Megakaryocytes, NK cells, and Neutrophis in comparison to the HC group. The CellChat analysis findings unveiled four distinct patterns for input signals and four patterns for output signals. The results of trajectory analysis revealed that the majority of cell subsets are positioned in a single developmental stage. Our research results comprehensively demonstrate the dynamic changes of immune cells during the onset of SLE, and identify specific V and J genes in TCR and BCR that can be used to expand our understanding of SLE. Full Text Additional Declarations No competing interests reported. Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-3814377","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":264362661,"identity":"b85e7bfc-a89c-44b3-b1cc-b9b2daccbea1","order_by":0,"name":"Hao Liu","email":"","orcid":"","institution":"Central Laboratory of Guizhou Aerospace Hospital","correspondingAuthor":false,"submittingAuthor":false,"prefix":"","firstName":"Hao","middleName":"","lastName":"Liu","suffix":""},{"id":264362662,"identity":"cdb1452a-7402-431b-98f6-53c2d79cf5eb","order_by":1,"name":"Yadong Gong","email":"","orcid":"","institution":"Central Laboratory of Guizhou Aerospace Hospital","correspondingAuthor":false,"submittingAuthor":false,"prefix":"","firstName":"Yadong","middleName":"","lastName":"Gong","suffix":""},{"id":264362663,"identity":"dddf08b9-a31c-44fe-8d8e-f63ec48239f2","order_by":2,"name":"Mubo Liu","email":"","orcid":"","institution":"Central Laboratory of Guizhou Aerospace Hospital","correspondingAuthor":false,"submittingAuthor":false,"prefix":"","firstName":"Mubo","middleName":"","lastName":"Liu","suffix":""},{"id":264362664,"identity":"57ac20c3-cbd4-45da-8f8c-a0fbfb7e08da","order_by":3,"name":"Ran Xiao","email":"","orcid":"","institution":"Central Laboratory of Guizhou Aerospace Hospital","correspondingAuthor":false,"submittingAuthor":false,"prefix":"","firstName":"Ran","middleName":"","lastName":"Xiao","suffix":""},{"id":264362665,"identity":"3aeece50-72a5-44c6-a096-ccc3bfa200df","order_by":4,"name":"Ma Qingqing","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAAA8ElEQVRIiWNgGAWjYJCCA0DMw8DA2HDgQ4WEnDwJWpgPPpxxxsLYsIF4y9iSjXnbKhLBJuADuu2HNx4u+GUtY86/xkxy5jyJBMYG5oePbuDRYnYmreDwzL50HssZb8wkPm6TyGNnYDM2zsGn5UCOwWHensM8BjfOAG3ZJlHM2MDDJo1Xy/k3CC3SvHMkEhsOENJyA2gLzw+glvNtQO83EKXlWcFh3oZ0oC2gQD4mYWzYTMgv55M3f+b5Y21vcP4gMCpr6uTk2ZsfPsanBQgMGBjbmBkYJBKgfGb8yiFaGP4AlfEfIKx0FIyCUTAKRiYAAFA/VLLGuHplAAAAAElFTkSuQmCC","orcid":"","institution":"Central Laboratory of Guizhou Aerospace Hospital","correspondingAuthor":true,"submittingAuthor":false,"prefix":"","firstName":"Ma","middleName":"","lastName":"Qingqing","suffix":""}],"badges":[],"createdAt":"2023-12-28 01:59:15","currentVersionCode":1,"declarations":"","doi":"10.21203/rs.3.rs-3814377/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-3814377/v1","draftVersion":[],"editorialEvents":[],"editorialNote":"","failedWorkflow":false,"files":[{"id":49534404,"identity":"76d8a61e-ba0f-4a28-9285-f8592c8fcf51","added_by":"auto","created_at":"2024-01-12 15:07:38","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":1868256,"visible":true,"origin":"","legend":"","description":"","filename":"RevealingtheImmuneHeterogeneityinSystemicLupusErythematosusBasedonMultiOmicsDataAnalysis1.pdf","url":"https://assets-eu.researchsquare.com/files/rs-3814377/v1_covered_6ecc0d67-0ec2-45ba-9cb3-a42430453aa7.pdf"}],"financialInterests":"No competing interests reported.","formattedTitle":"Revealing the Immune Heterogeneity in Systemic Lupus Erythematosus Based on Multi-Omics Data Analysis","fulltext":[],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":true,"highlight":"","institution":"","isAcceptedByJournal":false,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":true,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true},"keywords":"","lastPublishedDoi":"10.21203/rs.3.rs-3814377/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-3814377/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003eSystemic lupus erythematosus is an autoimmune disorder characterized by a spectrum of clinical manifestations. 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