8-OHdG and NT-proBNP as complementary biomarkers in the postmortem diagnosis of acute ischemic heart disease

8-OHdG and NT-proBNP as complementary biomarkers in the postmortem diagnosis of acute ischemic heart disease | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Article 8-OHdG and NT-proBNP as complementary biomarkers in the postmortem diagnosis of acute ischemic heart disease Yumi Kuninaka, Yuko Ishida, Federica Grimaldi, Marco Irmici, Mizuho Nosaka, and 4 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-7359354/v1 This work is licensed under a CC BY 4.0 License Status: Published Journal Publication published 19 Feb, 2026 Read the published version in Scientific Reports → Version 1 posted 11 You are reading this latest preprint version Abstract Acute ischemic heart disease (AIHD) is a major cause of sudden cardiac death, yet postmortem diagnosis is difficult, particularly in early-phase ischemic injury or decomposed bodies. We investigated the diagnostic value of two molecular biomarkers—8-hydroxy-2′-deoxyguanosine (8-OHdG), indicating oxidative DNA damage, and N-terminal pro-brain natriuretic peptide (NT-proBNP), reflecting cardiac wall stress—in 67 human forensic autopsy cases (33 AIHD, 34 non-AIHD). Immunohistochemistry revealed significantly greater and more diffuse nuclear 8-OHdG expression in AIHD cardiomyocytes compared with controls. Postmortem blood NT-proBNP levels were also significantly higher in AIHD cases (2389.1 ± 499.6 pg/mL) than in non-AIHD cases (896.1 ± 190.1 pg/mL). No significant correlations were found between 8-OHdG expression and NT-proBNP levels, contraction band necrosis, postmortem interval, age, or sex, suggesting that these markers reflect distinct pathological processes—oxidative injury and hemodynamic stress. Both markers demonstrated postmortem stability, and their combined evaluation improved diagnostic confidence in cases with inconclusive histology. This dual-biomarker approach may enhance the accuracy of AIHD diagnosis in forensic practice and provide complementary insights into the molecular mechanisms underlying sudden cardiac death. Health sciences/Biomarkers Health sciences/Cardiology Health sciences/Diseases Health sciences/Medical research Forensic pathology 8-OHdG Acute ischemic heart diseases Postmortem diagnosis NT-proBNP Figures Figure 1 Figure 2 Figure 3 Figure 4 Figure 5 Introduction Acute ischemic heart disease (AIHD) is the principal cause of sudden cardiac death worldwide and presents substantial diagnostic challenges in both clinical and forensic practices. Classical histopathological markers of myocardial ischemia such as contraction band necrosis and wavy fibers provide useful postmortem evidence; however, they may be inconclusive in early-stage ischemic injury or decomposed bodies (1). Hence, identifying reliable molecular biomarkers is critical for enhancing diagnostic accuracy in forensic pathology. Oxidative stress plays a central role in ischemic cardiac injury, and results in mitochondrial dysfunction, lipid peroxidation, and DNA damage. One a notable oxidative DNA lesion, 8-hydroxy-2′-deoxyguanosine (8-OHdG), is a robust marker of oxidative DNA damage and has been linked to cardiovascular disease (CVD). Meta-analyses have confirmed the elevation of systemic 8OHdG levels in patients with myocardial infarction and atherosclerosis compared to those in healthy controls (2). In human myocardial infarction cases, increased serum and tissue 8OHdG have been correlated with apoptotic signaling, implicating it in acute ischemic processes. However, studies specifically analyzing myocardial 8OHdG in forensic autopsy samples remain limited, and postmortem stability remains under investigation. N-terminal pro-brain natriuretic peptide (NT-proBNP), a neurohormone released in response to ventricular wall stress, is a valuable biomarker in both clinical and forensic cardiology. Elevated NTproBNP levels are well-documented in heart failure and myocardial infarction, and its measurement in postmortem samples, especially pericardial and femoral blood, has demonstrated promise in diagnosing cardiac death (3). However, NTproBNP levels can also be influenced by renal dysfunction, hypertension, hemolysis, bacterial degradation, sampling sites, and agonal events (4–7). In the present study, we quantitatively evaluated myocardial 8OHdG expression and postmortem blood NTproBNP levels in AIHD forensic autopsy cases compared with those in nonAIHD controls. We further analyzed their associations with histopathological indicators, postmortem intervals (PMI), and demographic factors to clarify their diagnostic value and limitations. By assessing both oxidative DNA damage and neurohormonal activation, we aimed to deepen our understanding of the molecular events underlying sudden cardiac death from AIHD and to enhance the forensic diagnostic framework. Results Histopathological analysis in autopsy samples Histopathologically, HE staining showed that there was no specific histological sign of myocardial infarction in both non-AIHD and AIHD groups (Fig. 1 ). Mild or moderate congestion was almost always found in the heart of both groups. In some of both cases, eosinophilic alteration and contraction band necrosis of myocardial cells were also observed. These observations implied that there was no significant difference in histopathological alterations between the AIHD and non-AIHD groups. Enhanced intracardiac nuclear 8-OHdG expression in AIHD group Immunohistochemical analysis revealed intranuclear expression of 8-OHdG in cardimomyocytes in both groups. Subsequently, semi-quantitative analyse demonstrated that a more intense and diffuses expression of 8-OHdG in the nuclei of cardiomyocytes within the AIHD group than in the non-AIHD group (Fig. 2 A). Actually, the number of 8-OHdG + cells was significantly higher in AIHD group, compared with non-AIHD groups (Fig. 2 B, P = 4.96 − 10 ). Increased blood NT-proBNP level in AIHD group We measured blood NT-proBNP levels in non-AIHD and AIHD groups. A comparison of the blood NT-proBNP levels in the AIHD and non-AIHD groups showed that the former group showed the significantly higher level of blood NT-proBNP than latte one (Fig. 3 ; P = 0.0074; non-AIHD: 896.1 ± 190.1 pg/mL, AIHD: 2389.1 ± 499.6 pg/mL). No correlation between the 8-OHdG + cell number and contraction bands, age, sex, post mortem intervals (PMI), or NT-proBNP No significant differences were observed between 8-OHdG protein expression and the presence or absence of contractions (Fig. 4 A), age (Fig. 4 B), sex (Fig. 4 C), PMI (Fig. 4 D), or NT-proBNP levels (Fig. 4 E). Furthermore, when the analysis was restricted to the AIHD group, no significant differences were observed between 8-OHdG protein expression and the presence or absence of contractions (Fig. 5 A) or NT-proBNP levels (Fig. 5 B). Discussion Our immunohistochemical analyses revealed markedly increased and diffusely distributed nuclear expression of 8-OHdG in the cardiomyocytes of the AIHD group, compared to that in the non-AIHD group. Kasai showed 8-OHdG was a well-established marker of oxidative DNA damage and served as an indicator of cumulative oxidative stress at the cellular level [8]. Nuclear localization of 8-OHdG in myocardial tissue supports the hypothesis that oxidative injury to the cardiomyocyte genomes is a key event in the pathogenesis of AIHD [9–13]. In line with previous studies, these observations demonstrated the elevation of 8-OHdG levels in myocardial infarction, heart failure, and sudden cardiac death, implicating oxidative DNA damage as a potential mechanism for cardiomyocyte dysfunction and death [9, 10, 12, 14, 15]. Furthermore, our previous study showed a significant increase in the number of Nrf2 + cells in the AIHD group, indicating the up-regulation of antioxidant defense mechanisms in response to increased oxidative stress. Nrf2 is a redox-sensitive transcription factor that activates the expression of cytoprotective genes and is involved in myocardial response to oxidative damage [16, 17]. NT-proBNP is a cardiac neurohormone released in response to ventricular wall stress and is a widely accepted biomarker of heart failure and acute cardiac injury [18–21], and our present results also demonstrated significantly higher blood NT-proBNP levels in the AIHD group than in the non-AIHD one. Elevated NT-proBNP levels have also been observed in forensic autopsy cases of sudden cardiac death, highlighting its diagnostic utility even postmortem [22–24]. Despite the simultaneous increase in 8-OHdG and NT-proBNP levels in the AIHD group, no correlation was observed between these two markers. This lack of correlation suggests that while both markers reflect different aspects of cardiac injury, such as oxidative DNA damage versus hemodynamic stress, they may operate through largely independent pathophysiological pathways in the acute phase of ischemic cardiac injury. (3, 25). The absence of a correlation between 8-OHdG and contraction band necrosis, sex, age, postmortem interval (PMI), and NT-proBNP levels supports the notion that 8-OHdG expression is primarily driven by acute oxidative stress rather than by secondary systemic factors or postmortem changes. This highlights its potential as a relatively stable marker of oxidative myocardial injury in forensic pathology (12, 25). Moreover, since NT-proBNP can remain detectable postmortem and is resistant to hemolysis and degradation [5, 26], the combined assessment of 8-OHdG and NT-proBNP could offer a complementary approach for evaluating acute cardiac pathology in forensic settings. While 8-OHdG reflects intracellular molecular damage, NT-proBNP serves as a surrogate for systemic cardiac stress response. Future studies should focus on combined diagnostic accuracy, especially in cases with equivocal findings on gross and histological examination (3, 6). Some limitations should be considered when interpreting the findings of this study, particularly from a forensic pathology standpoint: although no significant correlation was observed between PMI and 8-OHdG expression, the effect of autolytic changes, ischemia-reperfusion delay, and tissue degradation over time cannot be entirely excluded (12). Oxidative DNA lesions, such as 8-OHdG, may be influenced by both agonal processes and postmortem oxidative events, potentially confounding interpretations in cases of prolonged PMI (27, 28). The absence of detailed clinical information regarding cardiac function, comorbidities, or medication use before death limits our ability to correlate biochemical findings with clinical status. For example, elevated NT-proBNP levels may be affected by chronic conditions such as renal insufficiency or hypertension, which could not be fully evaluated postmortem (26, 29). Agonal events (e.g., hypoxia, catecholamine surge, and seizures) prior to death may independently enhance oxidative stress and neurohormonal activation, thereby elevating both 8-OHdG and NT-proBNP levels, independent of AIHD. Without detailed circumstances of death or physiological monitoring, such influences cannot be ruled out (7, 30, 31). Although NT-proBNP is relatively stable postmortem, potential variations due to hemolysis, bacterial degradation, or redistribution from tissues may still occur. Additionally, the differences in blood sampling sites (central vs. peripheral) were not standardized and may have affected the measured concentrations (3, 4, 26). In summary, our findings underscore the potential forensic utility of combining 8-OHdG and NT-proBNP levels as complementary biomarkers for assessing acute ischemic cardiac injury. While each reflects distinct pathological processes, oxidative DNA damage and cardiac wall stress, their simultaneous evaluation may enhance diagnostic accuracy, particularly in cases with ambiguous morphological evidence. Despite the inherent limitations of postmortem analysis, these markers offer promising avenues for improving the postmortem diagnosis of AIHD and warrant further validation in larger, clinically characterized cohorts. Methods Antibody In this study, following monoclonal antibody (mAbs) were used for immunohistochemical analyses: mouse anti-human 8-OHdG mAb (clone N45.1; MOG-100P; Japan Institute for the Control of Aging, Shizuoka, Japan). Measurement of blood NT-proBNP NT-proBNP levels were measured using an electrochemiluminescence immunoassay (ECLIA) using blood samples obtained from forensic autopsy cases (Medic Co., Ltd., Shiga, Japan). Human heart tissue samples A total of 67 human forensic autopsy cases were included in this study (43 males; 24 females) with a PMI of less than 72 h, selected based on autopsy documents. The individual ages ranged from 29 to 100 years (mean age, 65.1 ± 2.2). In each case, the cause of death was carefully determined through a comprehensive examination, including complete autopsy, histology, toxicology, and diatom tests. The cases were divided into two groups: 33 AIHD cases with advanced sclerosis and/or stenosis in the coronary artery and 34 others, including drowning (17 cases), fire death (4 cases), hanging (3 cases), traumatic shock (3 cases), asphyxia (2 cases), severe head injury (2 cases), drug and poisoning (2 cases), and peritonitis (one case). Detailed profiles of all cases (sex, age, and PMI) are shown in Table 1 . The following study groups were selected: AIHD, 33 cases; non-AIHD, 34 cases. The AIHD group comprised sudden cardiac death with gross and/or histopathological signs of myocardial infarction. In all cases of AIHD, the cause of death was acute myocardial infarction, Inclusion criteria for this group were histopathological signs (HE staining) of early ischemia, such as contraction band necrosis, cardiomyocyte coagulation necrosis, polymorphonuclear leukocyte infiltration, and/or wavy fibers. Cases showing putrefaction, carbonization, traumatic injury to the heart, and cases after percutaneous coronary revascularization procedures and/or coronary artery bypass grafting were excluded. The control group (non-AIHD group), included 34 cases with no clinical history or macroscopic and microscopic findings of cardiac pathology from various causes of deaths, as described in Table 1 . Table 1. Forensic autopsy cases Histopathological analyses The heart samples were fixed in 4% formaldehyde buffered with PBS and embedded in paraffin. Sections were stained with hematoxylin-eosin (HE) for histological analysis. Immunohistochemical analysis was performed using an anti-8-OHdG mAb. The deparaffinized sections were immersed in 0.3% H 2 O 2 in methanol for 30 min to eliminate endogenous peroxidase activity. The sections were further incubated with PBS containing 1% normal goat serum and 1% BSA to reduce nonspecific reactions. Subsequently, the sections were incubated with primary antibodies at 4°C overnight. After incubation with Envision + System-HRP Labelled Polymer (Dako Denmark A/S, Glostrup, Denmark), the immune complexes were visualized using 3,3'-Diaminobenzidine (DAB) solution (FUJIFILM Wako, Osaka, Japan) according to the manufacturer’s instructions. As a negative control, the sections were incubated with normal mouse IgG instead of primary antibodies, and no positive signal was detected, indicating the specificity of the primary antibody. Morphometrical analysis Morphometry was performed for semiquantitative evaluation, as previously reported (16). For 8-OHdG, five high-power microscopic fields (× 400, 0.2 × 0.2 mm each) were randomly chosen in each section. The number of 8-OHdG + cells was counted, and the average number was evaluated for each heart specimen. Statistical analysis The AIHD and control groups were compared using a two-sided unpaired Student’s t test to identify significant differences ( P < 0.01 considered significant). Pearson's product-moment correlation coefficient was used for statistical analysis of each correlation. All statistical analyses were performed using Statcel3 software under the supervision of a medical statistician as previously described (16). Declarations Ethical approval This study was approved by the Research Ethics Committee of Wakayama Medical University (No. 3176). All the procedures were performed in accordance with the principles of the Declaration of Helsinki. Moreover, this study was conducted using past autopsy records, and informed consent was not obtained from bereaved family members for the use of these records. Therefore, we conducted this study in accordance with the "Ethical Guidelines for Medical Research Involving Human Subjects (enacted by the Ministry of Health, Labor, and Welfare in Japan), Section 12–1 (2) (a) (c).” This was a de-identified study using archived tissue obtained from judicial autopsy cases, and information on the implementation of the study was posted on our website ( https://www.wakayama-med.ac.jp/dept/igakubu/160420/index.html ). If they were requested to refuse the use of the samples for research, they were excluded from the study. In addition, the review board of the Research Ethics Committee of Wakayama Medical University waived the need for written informed consent from relatives of the individuals studied in accordance with national legislation and institutional requirements. Conflict of interests The authors declare that they have no conflict of interests. Funding This work was supported by Japan Society for the Promotion of Science, Grant-in-Aid for Challenging Research (Pioneering) 25K21796 and (Exploratory) 22K19676 (Y. I.), Grant-in-Aid for Challenging Research (Pioneering) 23K17445 and Grant-in-Aid Scientific Research (B) 23K24624 (T. K.), Grant-in-Aid Scientific Research (C) 23K09770 (Y. K.), and Grant-in-Aid Scientific Research (C) 25K13658 (M. N.). Author Contribution Conceptualization: Y. I. and T. K.; Formal Analysis: F. G., M. I., and M. M.; Funding Acquisition: Y. K., M. N., and T. K.; Investigation: F. G., M. I. and Y. I.; Project Administration: T. K.; Validation: P. F. and S. P.; Writing - Original Draft Preparation: Y. I.; Writing - Review and Editing: T. K. Acknowledgements We sincerely thank Ms. Mariko Kawaguchi for her technical assistance. Data Availability The authors declare that all data are available in the article file, or available from the corresponding author (Toshikazu Kondo) upon reasonable request. References Michaud, K. et al. Diagnosis of myocardial infarction at autopsy: AECVP reappraisal in the light of the current clinical classification. Virchows Arch. 476 (2), 179–194 (2020). Minno, A. D. et al. 8-Hydroxy-2-Deoxyguanosine Levels and Cardiovascular Disease: A Systematic Review and Meta-Analysis of the Literature. Antioxid. Redox Signal. 24 (10), 548–555 (2016). Palmiere, C. et al. Cardiac troponins and NT-proBNP in the forensic setting: Overview of sampling site, postmortem interval, cardiopulmonary resuscitation, and review of the literature. Forensic Sci. Int. 282 , 211–218 (2018). Sabatasso, S. et al. Sensitivity and specificity of NT-proBNP to detect heart failure at post mortem examination. Int. J. Legal Med. 125 (6), 849–856 (2011). Cao, Z., Jia, Y. & Zhu, B. BNP and NT-proBNP as Diagnostic Biomarkers for Cardiac Dysfunction in Both Clinical and Forensic Medicine. Int. J. Mol. Sci. 20 (8), 1820 (2019). Kutlu, E. et al. Significance of postmortem biomarkers and multimarker strategy in sudden cardiac death. Leg. Med. (Tokyo) :61 :102212. (2023). Cao, Z. et al. Evaluation of Agonal Cardiac Function for Sudden Cardiac Death in Forensic Medicine with Postmortem Brain Natriuretic Peptide (BNP) and NT-proBNP: A Meta-analysis. J. Forensic Sci. 65 (3), 686–691 (2020). Kasai, H. Analysis of a form of oxidative DNA damage, 8-hydroxy-2'-deoxyguanosine, as a marker of cellular oxidative stress during carcinogenesis. Mutat. Res. 387 (3), 147–163 (1997). Kroese, L. J. & Scheffer, P. G. 8-hydroxy-2'-deoxyguanosine and cardiovascular disease: a systematic review. Curr. Atheroscler Rep. 16 (11), 452 (2014). Kono, Y. et al. Elevated levels of oxidative DNA damage in serum and myocardium of patients with heart failure. Circ. J. 70 (8), 1001–1005 (2006). Himmetoglu, S., Dincer, Y., Bozcali, E., Vural, V. A. & Akcay, T. Oxidative DNA damage and antioxidant defense after reperfusion in acute myocardial infarction. J. Investig Med. 57 (4), 595–599 (2009). Cordis, G. A., Maulik, G., Bagchi, D., Riedel, W. & Das, D. K. Detection of oxidative DNA damage to ischemic reperfused rat hearts by 8-hydroxydeoxyguanosine formation. J. Mol. Cell. Cardiol. 30 (10), 1939–1944 (1998). Morihira, M. et al. Ischemic preconditioning enhances scavenging activity of reactive oxygen species and diminishes transmural difference of infarct size. Am. J. Physiol. Heart Circ. Physiol. 290 (2), H577–H583 (2006). Minno, A. D. et al. 8-Hydroxy-2-deoxyguanosine levels and heart failure: A systematic review and meta-analysis of the literature. Nutr. Metab. Cardiovasc. Dis. 27 (3), 201–208 (2017). Watanabe, E. et al. Significance of 8-hydroxy-2'-deoxyguanosine levels in patients with idiopathic dilated cardiomyopathy. J. Card Fail. 12 (7), 527–532 (2006). Hiyamizu, S. et al. Forensic significance of intracardiac expressions of Nrf2 in acute myocardial ischemia. Sci. Rep. 14 (1), 4046 (2024). Itoh, K. et al. Keap1 regulates both cytoplasmic-nuclear shuttling and degradation of Nrf2 in response to electrophiles. Genes Cells . 8 (4), 379–391 (2003). Radwan, H., Selem, A. & Ghazal, K. Value of N-terminal pro brain natriuretic peptide in predicting prognosis and severity of coronary artery disease in acute coronary syndrome. J. Saudi Heart Assoc. 26 (4), 192–198 (2014). Salama, R., El-Moniem, A., El-Hefney, N. & Samor, T. N-terminal pro-BNP in acute coronary syndrome patients with ST elevation (STE-ACS) versus non ST elevation (NSTE-ACS). Int. J. Health Sci. (Qassim) . 5 (2 Suppl 1), 27–29 (2011). Krittayaphong, R. et al. Correlation Between NT-pro BNP levels and left ventricular wall stress, sphericity index and extent of myocardial damage: a magnetic resonance imaging study. J. Card Fail. 14 (8), 687–694 (2008). Weber, M. & Hamm, C. Role of B-type natriuretic peptide (BNP) and NT-proBNP in clinical routine. Heart 92 (6), 843–849 (2006). Riezebos, R. K., Ronner, E., Tijssen, J. G. P. & Laarman, G-J. NT-ProBNP serum levels reflect severity and extent of ischemia in patients admitted with non-ST-elevation acute coronary syndrome. Acute Card Care . 8 (1), 51–57 (2006). Sarak, T. & Karadeniz, M. The relationship between serum NT-proBNP levels and severity of coronary artery disease assessed by SYNTAX score in patients with acute myocardial infarction. Turk. J. Med. Sci. 49 (5), 1366–1373 (2019). Jering, K. S. et al. Prognostic Importance of NT-proBNP (N-Terminal Pro-B-Type Natriuretic Peptide) Following High-Risk Myocardial Infarction in the PARADISE-MI Trial. Circ. Heart Fail. 16 (5), e010259 (2023). Takasu, S., Matsumoto, S., Kanto, Y., Kodama, S. & Iwadate, K. Postmortem urine concentration of N-terminal pro-brain natriuretic peptide in relation to the cause of death. Forensic Sci. Int. 306 , 110079 (2020). Michaud, K. et al. Evaluation of postmortem measurement of NT-proBNP as a marker for cardiac function. Int. J. Legal Med. 122 (5), 415–420 (2008). Harish, G. et al. Glutathione metabolism is modulated by postmortem interval, gender difference and agonal state in postmortem human brains. Neurochem Int. 59 (7), 1029–1042 (2011). Silva, A. R. T. et al. Repair of oxidative DNA damage, cell-cycle regulation and neuronal death may influence the clinical manifestation of Alzheimer's disease. PLoS One . 9 (6), e99897 (2014). Tettamanti, C., Hervet, T., Grabherr, S. & Palmiere, C. Elevation of NT-proBNP and cardiac troponins in sepsis-related deaths: a forensic perspective. Int. J. Legal Med. 130 (4), 1035–1043 (2016). Fukuda, M. et al. The evaluation of oxidative DNA damage in children with brain damage using 8-hydroxydeoxyguanosine levels. Brain Dev. 30 (2), 131–136 (2008). Rosato, E. et al. Forensic Biochemical Markers to Evaluate the Agonal Period: A Literature Review. Molecules 26 (11), 3259 (2021). Additional Declarations No competing interests reported. Cite Share Download PDF Status: Published Journal Publication published 19 Feb, 2026 Read the published version in Scientific Reports → Version 1 posted Editorial decision: Revision requested 16 Oct, 2025 Reviews received at journal 19 Sep, 2025 Reviewers agreed at journal 12 Sep, 2025 Reviewers agreed at journal 12 Sep, 2025 Reviews received at journal 03 Sep, 2025 Reviewers agreed at journal 29 Aug, 2025 Reviewers invited by journal 27 Aug, 2025 Editor invited by journal 14 Aug, 2025 Editor assigned by journal 14 Aug, 2025 Submission checks completed at journal 13 Aug, 2025 First submitted to journal 12 Aug, 2025 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-7359354","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Article","associatedPublications":[],"authors":[{"id":507653144,"identity":"09eb8108-b1db-4820-8c6d-0b67a89b45b4","order_by":0,"name":"Yumi Kuninaka","email":"","orcid":"","institution":"Wakayama Medical University","correspondingAuthor":false,"prefix":"","firstName":"Yumi","middleName":"","lastName":"Kuninaka","suffix":""},{"id":507653145,"identity":"d0239b32-e692-4743-bb52-9c784543bdb9","order_by":1,"name":"Yuko Ishida","email":"","orcid":"","institution":"Wakayama Medical University","correspondingAuthor":false,"prefix":"","firstName":"Yuko","middleName":"","lastName":"Ishida","suffix":""},{"id":507653146,"identity":"35858c0b-ef71-4f18-9062-e910443c69ba","order_by":2,"name":"Federica Grimaldi","email":"","orcid":"","institution":"Wakayama Medical University","correspondingAuthor":false,"prefix":"","firstName":"Federica","middleName":"","lastName":"Grimaldi","suffix":""},{"id":507653147,"identity":"aade56cf-8e3d-499d-ab99-8a15d431ff0b","order_by":3,"name":"Marco Irmici","email":"","orcid":"","institution":"Wakayama Medical University","correspondingAuthor":false,"prefix":"","firstName":"Marco","middleName":"","lastName":"Irmici","suffix":""},{"id":507653148,"identity":"446f2c78-4559-4310-8514-1b1754c13186","order_by":4,"name":"Mizuho Nosaka","email":"","orcid":"","institution":"Wakayama Medical University","correspondingAuthor":false,"prefix":"","firstName":"Mizuho","middleName":"","lastName":"Nosaka","suffix":""},{"id":507653149,"identity":"2377c748-08cb-44ce-9079-2dd7d89dc0b5","order_by":5,"name":"Momoko Munekawa","email":"","orcid":"","institution":"Wakayama Medical University","correspondingAuthor":false,"prefix":"","firstName":"Momoko","middleName":"","lastName":"Munekawa","suffix":""},{"id":507653150,"identity":"8b5cfe45-c634-4516-8a91-e56b82beea31","order_by":6,"name":"Susi Pelotti","email":"","orcid":"","institution":"University of Bologna","correspondingAuthor":false,"prefix":"","firstName":"Susi","middleName":"","lastName":"Pelotti","suffix":""},{"id":507653151,"identity":"2562b1e5-c196-44e1-8877-d6a9cd6fc80a","order_by":7,"name":"Paolo Fais","email":"","orcid":"","institution":"University of Bologna","correspondingAuthor":false,"prefix":"","firstName":"Paolo","middleName":"","lastName":"Fais","suffix":""},{"id":507653152,"identity":"bdf4f9db-8789-4c4b-8dfd-cadd8fc0506d","order_by":8,"name":"Toshikazu Kondo","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAABC0lEQVRIiWNgGAWjYBACCQi2YWDgQQgagAhm/FoS0kjXchhTC04g2d578MbHH+cT+3sOP5P4mWPHoNvevIHhRw0DuzkOLdI855ItZyTcTpxxts1MsndbMoPZmWMFjD3HGJgtG7BrkZPIMZPmSbid23CewUyCdxtz/bYbOQYMvA0MzAYH8Go5lzv/PPs3yb/b6hnM7r8xYPyLR4s0RMuB3A1ne8ykebcdZjC7wWPAjM8WyZ4zxpYz0pLrN545U2wtu+040C9pBYdljkng9IvE8R7DGx9s7IzlzqRvvPl2WzWD2fHDGx++qbFJxhViyIBFAsYCOkkiGX/sQADzB2SeHTFaRsEoGAWjYEQAAFZsWc9ouE/LAAAAAElFTkSuQmCC","orcid":"","institution":"Wakayama Medical University","correspondingAuthor":true,"prefix":"","firstName":"Toshikazu","middleName":"","lastName":"Kondo","suffix":""}],"badges":[],"createdAt":"2025-08-12 22:38:16","currentVersionCode":1,"declarations":"","doi":"10.21203/rs.3.rs-7359354/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-7359354/v1","draftVersion":[],"editorialEvents":[{"content":"https://doi.org/10.1038/s41598-026-38797-w","type":"published","date":"2026-02-19T15:59:32+00:00"}],"editorialNote":"","failedWorkflow":false,"files":[{"id":90543258,"identity":"f263f939-2ea4-4e20-a1e6-a6aef7522f8d","added_by":"auto","created_at":"2025-09-04 00:10:06","extension":"jpg","order_by":1,"title":"Figure 1","display":"","copyAsset":false,"role":"figure","size":2569746,"visible":true,"origin":"","legend":"\u003cp\u003eHistopathological analysis of the human hearts (HE staining). Representative results from non-AIHD and AIHD groups were shown. (A) Drowing case 1, (B) Drowing case 2, (C) Hanging case 1, (D) AIHD case 1, (E) AIHD case 2, (F) AIHD case 3. Sbars, 50 μm.\u003c/p\u003e","description":"","filename":"image1.jpg","url":"https://assets-eu.researchsquare.com/files/rs-7359354/v1/3f9975c68c1f50311988f50f.jpg"},{"id":90544318,"identity":"3e95f296-6ae6-49ff-8982-2506c47abfbe","added_by":"auto","created_at":"2025-09-04 00:18:06","extension":"jpg","order_by":2,"title":"Figure 2","display":"","copyAsset":false,"role":"figure","size":196085,"visible":true,"origin":"","legend":"\u003cp\u003eExpression of 8-OHdG in the human heart. (A) Immunohistochemical analysis using anti-8-OHdG. Representative results from hearts of non-AIHD and AIHD groups are shown. (B) Morphometrical analysis was performed to measure the number of 8-OHdG\u003csup\u003e+\u003c/sup\u003e cells in each group. Mean and standard error of the number of 8-OHdG\u003csup\u003e+\u003c/sup\u003e cells per high power field (hpf) in each group. **\u003cem\u003eP\u003c/em\u003e \u0026lt; 0.01 indicates a statistically significant difference.\u003c/p\u003e","description":"","filename":"image2.jpg","url":"https://assets-eu.researchsquare.com/files/rs-7359354/v1/fd0a45b0825fca7d1b9070b6.jpg"},{"id":90543251,"identity":"ce43182a-9c6f-49f4-9ad7-a288877ffc6b","added_by":"auto","created_at":"2025-09-04 00:10:06","extension":"jpg","order_by":3,"title":"Figure 3","display":"","copyAsset":false,"role":"figure","size":85723,"visible":true,"origin":"","legend":"\u003cp\u003eThe blood NT-proBNP levels of non-AIHD and AIHD groups. Bars indicate the mean blood NT-proBNP levels in the non-AIHD and AIHD groups, respectively. **\u003cem\u003eP\u003c/em\u003e\u0026lt; 0.01 indicates a statistically significant difference.\u003c/p\u003e","description":"","filename":"image3.jpg","url":"https://assets-eu.researchsquare.com/files/rs-7359354/v1/8696511ba3f585fac398aa68.jpg"},{"id":90543254,"identity":"1560dab3-4004-46a8-9f44-1439d751d8cd","added_by":"auto","created_at":"2025-09-04 00:10:06","extension":"jpg","order_by":4,"title":"Figure 4","display":"","copyAsset":false,"role":"figure","size":456487,"visible":true,"origin":"","legend":"\u003cp\u003eCorrelation between contraction bands, age, sex, PMI, and NT-proBNP levels and the occurrence of the occurrence of 8-OHdG\u003csup\u003e+\u003c/sup\u003e cells in all cases (non-AIHD and AIHD cases). Pearson's product-moment correlation coefficients were described. (A) Contraction bands, (B) Age, (C) Sex, (D) PMI, (E) NT-proBNP.\u003c/p\u003e","description":"","filename":"image4.jpg","url":"https://assets-eu.researchsquare.com/files/rs-7359354/v1/81e2347412062fb3f4401b29.jpg"},{"id":90544321,"identity":"45772fd7-5ee2-4367-8b78-c2f9e4b0c815","added_by":"auto","created_at":"2025-09-04 00:18:07","extension":"jpg","order_by":5,"title":"Figure 5","display":"","copyAsset":false,"role":"figure","size":194106,"visible":true,"origin":"","legend":"\u003cp\u003eCorrelation between contraction bands and the occurrence of 8-OHdG\u003csup\u003e+\u003c/sup\u003e cells and between NT-proBNP levels and the occurrence of 8-OHdG\u003csup\u003e+\u003c/sup\u003e cells in AIHD cases only. Pearson's product-moment correlation coefficients were described. (A) Contraction bands, (B) NT-proBNP levels.\u003c/p\u003e","description":"","filename":"image5.jpg","url":"https://assets-eu.researchsquare.com/files/rs-7359354/v1/83be3ff29173188521f66a60.jpg"},{"id":103252081,"identity":"55eb7336-1075-4b2b-a0d6-cb1d77ccae5d","added_by":"auto","created_at":"2026-02-23 16:12:54","extension":"pdf","order_by":0,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":4195045,"visible":true,"origin":"","legend":"","description":"","filename":"manuscript.pdf","url":"https://assets-eu.researchsquare.com/files/rs-7359354/v1/9cda528f-0f2a-48a7-8a74-265f76a3aa12.pdf"}],"financialInterests":"No competing interests reported.","formattedTitle":"8-OHdG and NT-proBNP as complementary biomarkers in the postmortem diagnosis of acute ischemic heart disease","fulltext":[{"header":"Introduction","content":"\u003cp\u003eAcute ischemic heart disease (AIHD) is the principal cause of sudden cardiac death worldwide and presents substantial diagnostic challenges in both clinical and forensic practices. Classical histopathological markers of myocardial ischemia such as contraction band necrosis and wavy fibers provide useful postmortem evidence; however, they may be inconclusive in early-stage ischemic injury or decomposed bodies (1). Hence, identifying reliable molecular biomarkers is critical for enhancing diagnostic accuracy in forensic pathology.\u003c/p\u003e\u003cp\u003eOxidative stress plays a central role in ischemic cardiac injury, and results in mitochondrial dysfunction, lipid peroxidation, and DNA damage. One a notable oxidative DNA lesion, 8-hydroxy-2\u0026prime;-deoxyguanosine (8-OHdG), is a robust marker of oxidative DNA damage and has been linked to cardiovascular disease (CVD). Meta-analyses have confirmed the elevation of systemic 8OHdG levels in patients with myocardial infarction and atherosclerosis compared to those in healthy controls (2). In human myocardial infarction cases, increased serum and tissue 8OHdG have been correlated with apoptotic signaling, implicating it in acute ischemic processes. However, studies specifically analyzing myocardial 8OHdG in forensic autopsy samples remain limited, and postmortem stability remains under investigation.\u003c/p\u003e\u003cp\u003eN-terminal pro-brain natriuretic peptide (NT-proBNP), a neurohormone released in response to ventricular wall stress, is a valuable biomarker in both clinical and forensic cardiology. Elevated NTproBNP levels are well-documented in heart failure and myocardial infarction, and its measurement in postmortem samples, especially pericardial and femoral blood, has demonstrated promise in diagnosing cardiac death (3). However, NTproBNP levels can also be influenced by renal dysfunction, hypertension, hemolysis, bacterial degradation, sampling sites, and agonal events (4\u0026ndash;7).\u003c/p\u003e\u003cp\u003eIn the present study, we quantitatively evaluated myocardial 8OHdG expression and postmortem blood NTproBNP levels in AIHD forensic autopsy cases compared with those in nonAIHD controls. We further analyzed their associations with histopathological indicators, postmortem intervals (PMI), and demographic factors to clarify their diagnostic value and limitations. By assessing both oxidative DNA damage and neurohormonal activation, we aimed to deepen our understanding of the molecular events underlying sudden cardiac death from AIHD and to enhance the forensic diagnostic framework.\u003c/p\u003e"},{"header":"Results","content":"\u003cdiv id=\"Sec3\" class=\"Section2\"\u003e\u003ch2\u003eHistopathological analysis in autopsy samples\u003c/h2\u003e\u003cp\u003eHistopathologically, HE staining showed that there was no specific histological sign of myocardial infarction in both non-AIHD and AIHD groups (Fig.\u0026nbsp;\u003cspan refid=\"Fig1\" class=\"InternalRef\"\u003e1\u003c/span\u003e). Mild or moderate congestion was almost always found in the heart of both groups. In some of both cases, eosinophilic alteration and contraction band necrosis of myocardial cells were also observed. These observations implied that there was no significant difference in histopathological alterations between the AIHD and non-AIHD groups.\u003c/p\u003e\u003cp\u003e\u003c/p\u003e\u003c/div\u003e\n\u003ch3\u003eEnhanced intracardiac nuclear 8-OHdG expression in AIHD group\u003c/h3\u003e\n\u003cp\u003eImmunohistochemical analysis revealed intranuclear expression of 8-OHdG in cardimomyocytes in both groups. Subsequently, semi-quantitative analyse demonstrated that a more intense and diffuses expression of 8-OHdG in the nuclei of cardiomyocytes within the AIHD group than in the non-AIHD group (Fig.\u0026nbsp;\u003cspan refid=\"Fig2\" class=\"InternalRef\"\u003e2\u003c/span\u003eA). Actually, the number of 8-OHdG\u003csup\u003e+\u003c/sup\u003e cells was significantly higher in AIHD group, compared with non-AIHD groups (Fig.\u0026nbsp;\u003cspan refid=\"Fig2\" class=\"InternalRef\"\u003e2\u003c/span\u003eB, P\u0026thinsp;=\u0026thinsp;4.96\u003csup\u003e\u0026minus;\u0026thinsp;10\u003c/sup\u003e).\u003c/p\u003e\u003cp\u003e\u003c/p\u003e\n\u003ch3\u003eIncreased blood NT-proBNP level in AIHD group\u003c/h3\u003e\n\u003cp\u003eWe measured blood NT-proBNP levels in non-AIHD and AIHD groups. A comparison of the blood NT-proBNP levels in the AIHD and non-AIHD groups showed that the former group showed the significantly higher level of blood NT-proBNP than latte one (Fig.\u0026nbsp;\u003cspan refid=\"Fig3\" class=\"InternalRef\"\u003e3\u003c/span\u003e; \u003cem\u003eP\u003c/em\u003e\u0026thinsp;=\u0026thinsp;0.0074; non-AIHD: 896.1\u0026thinsp;\u0026plusmn;\u0026thinsp;190.1 pg/mL, AIHD: 2389.1\u0026thinsp;\u0026plusmn;\u0026thinsp;499.6 pg/mL).\u003c/p\u003e\u003cp\u003e\u003c/p\u003e\u003cp\u003e\u003cb\u003eNo correlation between the 8-OHdG\u003c/b\u003e\u003csup\u003e\u003cb\u003e+\u003c/b\u003e\u003c/sup\u003e \u003cb\u003ecell number and contraction bands, age, sex, post mortem intervals (PMI), or NT-proBNP\u003c/b\u003e\u003c/p\u003e\u003cp\u003eNo significant differences were observed between 8-OHdG protein expression and the presence or absence of contractions (Fig.\u0026nbsp;\u003cspan refid=\"Fig4\" class=\"InternalRef\"\u003e4\u003c/span\u003eA), age (Fig.\u0026nbsp;\u003cspan refid=\"Fig4\" class=\"InternalRef\"\u003e4\u003c/span\u003eB), sex (Fig.\u0026nbsp;\u003cspan refid=\"Fig4\" class=\"InternalRef\"\u003e4\u003c/span\u003eC), PMI (Fig.\u0026nbsp;\u003cspan refid=\"Fig4\" class=\"InternalRef\"\u003e4\u003c/span\u003eD), or NT-proBNP levels (Fig.\u0026nbsp;\u003cspan refid=\"Fig4\" class=\"InternalRef\"\u003e4\u003c/span\u003eE). Furthermore, when the analysis was restricted to the AIHD group, no significant differences were observed between 8-OHdG protein expression and the presence or absence of contractions (Fig.\u0026nbsp;\u003cspan refid=\"Fig5\" class=\"InternalRef\"\u003e5\u003c/span\u003eA) or NT-proBNP levels (Fig.\u0026nbsp;\u003cspan refid=\"Fig5\" class=\"InternalRef\"\u003e5\u003c/span\u003eB).\u003c/p\u003e\u003cp\u003e\u003c/p\u003e\u003cp\u003e\u003c/p\u003e"},{"header":"Discussion","content":"\u003cp\u003eOur immunohistochemical analyses revealed markedly increased and diffusely distributed nuclear expression of 8-OHdG in the cardiomyocytes of the AIHD group, compared to that in the non-AIHD group. Kasai showed 8-OHdG was a well-established marker of oxidative DNA damage and served as an indicator of cumulative oxidative stress at the cellular level [8]. Nuclear localization of 8-OHdG in myocardial tissue supports the hypothesis that oxidative injury to the cardiomyocyte genomes is a key event in the pathogenesis of AIHD [9\u0026ndash;13]. In line with previous studies, these observations demonstrated the elevation of 8-OHdG levels in myocardial infarction, heart failure, and sudden cardiac death, implicating oxidative DNA damage as a potential mechanism for cardiomyocyte dysfunction and death [9, 10, 12, 14, 15]. Furthermore, our previous study showed a significant increase in the number of Nrf2\u003csup\u003e+\u003c/sup\u003e cells in the AIHD group, indicating the up-regulation of antioxidant defense mechanisms in response to increased oxidative stress. Nrf2 is a redox-sensitive transcription factor that activates the expression of cytoprotective genes and is involved in myocardial response to oxidative damage [16, 17].\u003c/p\u003e\u003cp\u003eNT-proBNP is a cardiac neurohormone released in response to ventricular wall stress and is a widely accepted biomarker of heart failure and acute cardiac injury [18\u0026ndash;21], and our present results also demonstrated significantly higher blood NT-proBNP levels in the AIHD group than in the non-AIHD one. Elevated NT-proBNP levels have also been observed in forensic autopsy cases of sudden cardiac death, highlighting its diagnostic utility even postmortem [22\u0026ndash;24]. Despite the simultaneous increase in 8-OHdG and NT-proBNP levels in the AIHD group, no correlation was observed between these two markers. This lack of correlation suggests that while both markers reflect different aspects of cardiac injury, such as oxidative DNA damage versus hemodynamic stress, they may operate through largely independent pathophysiological pathways in the acute phase of ischemic cardiac injury. (3, 25). The absence of a correlation between 8-OHdG and contraction band necrosis, sex, age, postmortem interval (PMI), and NT-proBNP levels supports the notion that 8-OHdG expression is primarily driven by acute oxidative stress rather than by secondary systemic factors or postmortem changes. This highlights its potential as a relatively stable marker of oxidative myocardial injury in forensic pathology (12, 25). Moreover, since NT-proBNP can remain detectable postmortem and is resistant to hemolysis and degradation [5, 26], the combined assessment of 8-OHdG and NT-proBNP could offer a complementary approach for evaluating acute cardiac pathology in forensic settings. While 8-OHdG reflects intracellular molecular damage, NT-proBNP serves as a surrogate for systemic cardiac stress response. Future studies should focus on combined diagnostic accuracy, especially in cases with equivocal findings on gross and histological examination (3, 6).\u003c/p\u003e\u003cp\u003eSome limitations should be considered when interpreting the findings of this study, particularly from a forensic pathology standpoint: although no significant correlation was observed between PMI and 8-OHdG expression, the effect of autolytic changes, ischemia-reperfusion delay, and tissue degradation over time cannot be entirely excluded (12). Oxidative DNA lesions, such as 8-OHdG, may be influenced by both agonal processes and postmortem oxidative events, potentially confounding interpretations in cases of prolonged PMI (27, 28). The absence of detailed clinical information regarding cardiac function, comorbidities, or medication use before death limits our ability to correlate biochemical findings with clinical status. For example, elevated NT-proBNP levels may be affected by chronic conditions such as renal insufficiency or hypertension, which could not be fully evaluated postmortem (26, 29). Agonal events (e.g., hypoxia, catecholamine surge, and seizures) prior to death may independently enhance oxidative stress and neurohormonal activation, thereby elevating both 8-OHdG and NT-proBNP levels, independent of AIHD. Without detailed circumstances of death or physiological monitoring, such influences cannot be ruled out (7, 30, 31). Although NT-proBNP is relatively stable postmortem, potential variations due to hemolysis, bacterial degradation, or redistribution from tissues may still occur. Additionally, the differences in blood sampling sites (central vs. peripheral) were not standardized and may have affected the measured concentrations (3, 4, 26).\u003c/p\u003e\u003cp\u003eIn summary, our findings underscore the potential forensic utility of combining 8-OHdG and NT-proBNP levels as complementary biomarkers for assessing acute ischemic cardiac injury. While each reflects distinct pathological processes, oxidative DNA damage and cardiac wall stress, their simultaneous evaluation may enhance diagnostic accuracy, particularly in cases with ambiguous morphological evidence. Despite the inherent limitations of postmortem analysis, these markers offer promising avenues for improving the postmortem diagnosis of AIHD and warrant further validation in larger, clinically characterized cohorts.\u003c/p\u003e"},{"header":"Methods","content":"\u003cdiv id=\"Sec8\" class=\"Section2\"\u003e\u003ch2\u003eAntibody\u003c/h2\u003e\u003cp\u003eIn this study, following monoclonal antibody (mAbs) were used for immunohistochemical analyses: mouse anti-human 8-OHdG mAb (clone N45.1; MOG-100P; Japan Institute for the Control of Aging, Shizuoka, Japan).\u003c/p\u003e\u003c/div\u003e\n\u003ch3\u003eMeasurement of blood NT-proBNP\u003c/h3\u003e\n\u003cp\u003eNT-proBNP levels were measured using an electrochemiluminescence immunoassay (ECLIA) using blood samples obtained from forensic autopsy cases (Medic Co., Ltd., Shiga, Japan).\u003c/p\u003e\n\u003ch3\u003eHuman heart tissue samples\u003c/h3\u003e\n\u003cp\u003eA total of 67 human forensic autopsy cases were included in this study (43 males; 24 females) with a PMI of less than 72 h, selected based on autopsy documents. The individual ages ranged from 29 to 100 years (mean age, 65.1\u0026thinsp;\u0026plusmn;\u0026thinsp;2.2). In each case, the cause of death was carefully determined through a comprehensive examination, including complete autopsy, histology, toxicology, and diatom tests. The cases were divided into two groups: 33 AIHD cases with advanced sclerosis and/or stenosis in the coronary artery and 34 others, including drowning (17 cases), fire death (4 cases), hanging (3 cases), traumatic shock (3 cases), asphyxia (2 cases), severe head injury (2 cases), drug and poisoning (2 cases), and peritonitis (one case). Detailed profiles of all cases (sex, age, and PMI) are shown in Table\u0026nbsp;\u003cspan refid=\"Tab1\" class=\"InternalRef\"\u003e1\u003c/span\u003e. The following study groups were selected: AIHD, 33 cases; non-AIHD, 34 cases. The AIHD group comprised sudden cardiac death with gross and/or histopathological signs of myocardial infarction. In all cases of AIHD, the cause of death was acute myocardial infarction, Inclusion criteria for this group were histopathological signs (HE staining) of early ischemia, such as contraction band necrosis, cardiomyocyte coagulation necrosis, polymorphonuclear leukocyte infiltration, and/or wavy fibers. Cases showing putrefaction, carbonization, traumatic injury to the heart, and cases after percutaneous coronary revascularization procedures and/or coronary artery bypass grafting were excluded. The control group (non-AIHD group), included 34 cases with no clinical history or macroscopic and microscopic findings of cardiac pathology from various causes of deaths, as described in Table\u0026nbsp;\u003cspan refid=\"Tab1\" class=\"InternalRef\"\u003e1\u003c/span\u003e.\u003c/p\u003e\u003cp\u003e\u003cstrong\u003eTable 1.\u003c/strong\u003e Forensic autopsy cases\u003c/p\u003e\n\u003cp\u003e\u003cimg src=\"data:image/png;base64,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\"\u003e\u003c/p\u003e\u003cdiv id=\"Sec11\" class=\"Section2\"\u003e\u003ch2\u003eHistopathological analyses\u003c/h2\u003e\u003cp\u003eThe heart samples were fixed in 4% formaldehyde buffered with PBS and embedded in paraffin. Sections were stained with hematoxylin-eosin (HE) for histological analysis. Immunohistochemical analysis was performed using an anti-8-OHdG mAb. The deparaffinized sections were immersed in 0.3% H\u003csub\u003e2\u003c/sub\u003eO\u003csub\u003e2\u003c/sub\u003e in methanol for 30 min to eliminate endogenous peroxidase activity. The sections were further incubated with PBS containing 1% normal goat serum and 1% BSA to reduce nonspecific reactions. Subsequently, the sections were incubated with primary antibodies at 4\u0026deg;C overnight. After incubation with Envision\u0026thinsp;+\u0026thinsp;System-HRP Labelled Polymer (Dako Denmark A/S, Glostrup, Denmark), the immune complexes were visualized using 3,3'-Diaminobenzidine (DAB) solution (FUJIFILM Wako, Osaka, Japan) according to the manufacturer\u0026rsquo;s instructions. As a negative control, the sections were incubated with normal mouse IgG instead of primary antibodies, and no positive signal was detected, indicating the specificity of the primary antibody.\u003c/p\u003e\u003c/div\u003e\u003cdiv id=\"Sec12\" class=\"Section2\"\u003e\u003ch2\u003eMorphometrical analysis\u003c/h2\u003e\u003cp\u003eMorphometry was performed for semiquantitative evaluation, as previously reported (16). For 8-OHdG, five high-power microscopic fields (\u0026times; 400, 0.2 \u0026times; 0.2 mm each) were randomly chosen in each section. The number of 8-OHdG\u003csup\u003e+\u003c/sup\u003e cells was counted, and the average number was evaluated for each heart specimen.\u003c/p\u003e\u003c/div\u003e\u003cdiv id=\"Sec13\" class=\"Section2\"\u003e\u003ch2\u003eStatistical analysis\u003c/h2\u003e\u003cp\u003eThe AIHD and control groups were compared using a two-sided unpaired Student\u0026rsquo;s \u003cem\u003et\u003c/em\u003e test to identify significant differences (\u003cem\u003eP\u003c/em\u003e\u0026thinsp;\u0026lt;\u0026thinsp;0.01 considered significant). Pearson's product-moment correlation coefficient was used for statistical analysis of each correlation. All statistical analyses were performed using Statcel3 software under the supervision of a medical statistician as previously described (16).\u003c/p\u003e\u003c/div\u003e"},{"header":"Declarations","content":"\u003cp\u003e\u003ch2\u003eEthical approval\u003c/h2\u003e\u003cp\u003e This study was approved by the Research Ethics Committee of Wakayama Medical University (No. 3176). All the procedures were performed in accordance with the principles of the Declaration of Helsinki. Moreover, this study was conducted using past autopsy records, and informed consent was not obtained from bereaved family members for the use of these records. Therefore, we conducted this study in accordance with the \"Ethical Guidelines for Medical Research Involving Human Subjects (enacted by the Ministry of Health, Labor, and Welfare in Japan), Section 12\u0026ndash;1 (2) (a) (c).\u0026rdquo; This was a de-identified study using archived tissue obtained from judicial autopsy cases, and information on the implementation of the study was posted on our website (\u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003ehttps://www.wakayama-med.ac.jp/dept/igakubu/160420/index.html\u003c/span\u003e\u003cspan address=\"https://www.wakayama-med.ac.jp/dept/igakubu/160420/index.html\" targettype=\"URL\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e). If they were requested to refuse the use of the samples for research, they were excluded from the study. In addition, the review board of the Research Ethics Committee of Wakayama Medical University waived the need for written informed consent from relatives of the individuals studied in accordance with national legislation and institutional requirements.\u003c/p\u003e\u003c/p\u003e\u003cp\u003e\u003ch2\u003eConflict of interests\u003c/h2\u003e\u003cp\u003eThe authors declare that they have no conflict of interests.\u003c/p\u003e\u003c/p\u003e\u003ch2\u003eFunding\u003c/h2\u003e\u003cp\u003eThis work was supported by Japan Society for the Promotion of Science, Grant-in-Aid for Challenging Research (Pioneering) 25K21796 and (Exploratory) 22K19676 (Y. I.), Grant-in-Aid for Challenging Research (Pioneering) 23K17445 and Grant-in-Aid Scientific Research (B) 23K24624 (T. K.), Grant-in-Aid Scientific Research (C) 23K09770 (Y. K.), and Grant-in-Aid Scientific Research (C) 25K13658 (M. N.).\u003c/p\u003e\u003ch2\u003eAuthor Contribution\u003c/h2\u003e\u003cp\u003eConceptualization: Y. I. and T. K.; Formal Analysis: F. G., M. I., and M. M.; Funding Acquisition: Y. K., M. N., and T. K.; Investigation: F. G., M. I. and Y. I.; Project Administration: T. K.; Validation: P. F. and S. P.; Writing - Original Draft Preparation: Y. I.; Writing - Review and Editing: T. K.\u003c/p\u003e\u003ch2\u003eAcknowledgements\u003c/h2\u003e\u003cp\u003eWe sincerely thank Ms. Mariko Kawaguchi for her technical assistance.\u003c/p\u003e\u003ch2\u003eData Availability\u003c/h2\u003e\u003cp\u003eThe authors declare that all data are available in the article file, or available from the corresponding author (Toshikazu Kondo) upon reasonable request.\u003c/p\u003e"},{"header":"References","content":"\u003col\u003e\u003cli\u003e\u003cspan\u003eMichaud, K. et al. Diagnosis of myocardial infarction at autopsy: AECVP reappraisal in the light of the current clinical classification. \u003cem\u003eVirchows Arch.\u003c/em\u003e \u003cb\u003e476\u003c/b\u003e (2), 179\u0026ndash;194 (2020).\u003c/span\u003e\u003c/li\u003e\u003cli\u003e\u003cspan\u003eMinno, A. D. et al. 8-Hydroxy-2-Deoxyguanosine Levels and Cardiovascular Disease: A Systematic Review and Meta-Analysis of the Literature. \u003cem\u003eAntioxid. Redox Signal.\u003c/em\u003e \u003cb\u003e24\u003c/b\u003e (10), 548\u0026ndash;555 (2016).\u003c/span\u003e\u003c/li\u003e\u003cli\u003e\u003cspan\u003ePalmiere, C. et al. Cardiac troponins and NT-proBNP in the forensic setting: Overview of sampling site, postmortem interval, cardiopulmonary resuscitation, and review of the literature. \u003cem\u003eForensic Sci. Int.\u003c/em\u003e \u003cb\u003e282\u003c/b\u003e, 211\u0026ndash;218 (2018).\u003c/span\u003e\u003c/li\u003e\u003cli\u003e\u003cspan\u003eSabatasso, S. et al. Sensitivity and specificity of NT-proBNP to detect heart failure at post mortem examination. \u003cem\u003eInt. J. Legal Med.\u003c/em\u003e \u003cb\u003e125\u003c/b\u003e (6), 849\u0026ndash;856 (2011).\u003c/span\u003e\u003c/li\u003e\u003cli\u003e\u003cspan\u003eCao, Z., Jia, Y. \u0026amp; Zhu, B. BNP and NT-proBNP as Diagnostic Biomarkers for Cardiac Dysfunction in Both Clinical and Forensic Medicine. \u003cem\u003eInt. J. Mol. Sci.\u003c/em\u003e \u003cb\u003e20\u003c/b\u003e (8), 1820 (2019).\u003c/span\u003e\u003c/li\u003e\u003cli\u003e\u003cspan\u003eKutlu, E. et al. Significance of postmortem biomarkers and multimarker strategy in sudden cardiac death. \u003cem\u003eLeg. Med. (Tokyo)\u003c/em\u003e :61 :102212. (2023).\u003c/span\u003e\u003c/li\u003e\u003cli\u003e\u003cspan\u003eCao, Z. et al. Evaluation of Agonal Cardiac Function for Sudden Cardiac Death in Forensic Medicine with Postmortem Brain Natriuretic Peptide (BNP) and NT-proBNP: A Meta-analysis. \u003cem\u003eJ. Forensic Sci.\u003c/em\u003e \u003cb\u003e65\u003c/b\u003e (3), 686\u0026ndash;691 (2020).\u003c/span\u003e\u003c/li\u003e\u003cli\u003e\u003cspan\u003eKasai, H. Analysis of a form of oxidative DNA damage, 8-hydroxy-2'-deoxyguanosine, as a marker of cellular oxidative stress during carcinogenesis. \u003cem\u003eMutat. Res.\u003c/em\u003e \u003cb\u003e387\u003c/b\u003e (3), 147\u0026ndash;163 (1997).\u003c/span\u003e\u003c/li\u003e\u003cli\u003e\u003cspan\u003eKroese, L. J. \u0026amp; Scheffer, P. G. 8-hydroxy-2'-deoxyguanosine and cardiovascular disease: a systematic review. \u003cem\u003eCurr. Atheroscler Rep.\u003c/em\u003e \u003cb\u003e16\u003c/b\u003e (11), 452 (2014).\u003c/span\u003e\u003c/li\u003e\u003cli\u003e\u003cspan\u003eKono, Y. et al. Elevated levels of oxidative DNA damage in serum and myocardium of patients with heart failure. \u003cem\u003eCirc. J.\u003c/em\u003e \u003cb\u003e70\u003c/b\u003e (8), 1001\u0026ndash;1005 (2006).\u003c/span\u003e\u003c/li\u003e\u003cli\u003e\u003cspan\u003eHimmetoglu, S., Dincer, Y., Bozcali, E., Vural, V. A. \u0026amp; Akcay, T. Oxidative DNA damage and antioxidant defense after reperfusion in acute myocardial infarction. \u003cem\u003eJ. Investig Med.\u003c/em\u003e \u003cb\u003e57\u003c/b\u003e (4), 595\u0026ndash;599 (2009).\u003c/span\u003e\u003c/li\u003e\u003cli\u003e\u003cspan\u003eCordis, G. A., Maulik, G., Bagchi, D., Riedel, W. \u0026amp; Das, D. K. Detection of oxidative DNA damage to ischemic reperfused rat hearts by 8-hydroxydeoxyguanosine formation. \u003cem\u003eJ. Mol. Cell. Cardiol.\u003c/em\u003e \u003cb\u003e30\u003c/b\u003e (10), 1939\u0026ndash;1944 (1998).\u003c/span\u003e\u003c/li\u003e\u003cli\u003e\u003cspan\u003eMorihira, M. et al. Ischemic preconditioning enhances scavenging activity of reactive oxygen species and diminishes transmural difference of infarct size. \u003cem\u003eAm. J. Physiol. Heart Circ. Physiol.\u003c/em\u003e \u003cb\u003e290\u003c/b\u003e (2), H577\u0026ndash;H583 (2006).\u003c/span\u003e\u003c/li\u003e\u003cli\u003e\u003cspan\u003eMinno, A. D. et al. 8-Hydroxy-2-deoxyguanosine levels and heart failure: A systematic review and meta-analysis of the literature. \u003cem\u003eNutr. Metab. Cardiovasc. Dis.\u003c/em\u003e \u003cb\u003e27\u003c/b\u003e (3), 201\u0026ndash;208 (2017).\u003c/span\u003e\u003c/li\u003e\u003cli\u003e\u003cspan\u003eWatanabe, E. et al. Significance of 8-hydroxy-2'-deoxyguanosine levels in patients with idiopathic dilated cardiomyopathy. \u003cem\u003eJ. Card Fail.\u003c/em\u003e \u003cb\u003e12\u003c/b\u003e (7), 527\u0026ndash;532 (2006).\u003c/span\u003e\u003c/li\u003e\u003cli\u003e\u003cspan\u003eHiyamizu, S. et al. Forensic significance of intracardiac expressions of Nrf2 in acute myocardial ischemia. \u003cem\u003eSci. Rep.\u003c/em\u003e \u003cb\u003e14\u003c/b\u003e (1), 4046 (2024).\u003c/span\u003e\u003c/li\u003e\u003cli\u003e\u003cspan\u003eItoh, K. et al. Keap1 regulates both cytoplasmic-nuclear shuttling and degradation of Nrf2 in response to electrophiles. \u003cem\u003eGenes Cells\u003c/em\u003e. \u003cb\u003e8\u003c/b\u003e (4), 379\u0026ndash;391 (2003).\u003c/span\u003e\u003c/li\u003e\u003cli\u003e\u003cspan\u003eRadwan, H., Selem, A. \u0026amp; Ghazal, K. Value of N-terminal pro brain natriuretic peptide in predicting prognosis and severity of coronary artery disease in acute coronary syndrome. \u003cem\u003eJ. Saudi Heart Assoc.\u003c/em\u003e \u003cb\u003e26\u003c/b\u003e (4), 192\u0026ndash;198 (2014).\u003c/span\u003e\u003c/li\u003e\u003cli\u003e\u003cspan\u003eSalama, R., El-Moniem, A., El-Hefney, N. \u0026amp; Samor, T. N-terminal pro-BNP in acute coronary syndrome patients with ST elevation (STE-ACS) versus non ST elevation (NSTE-ACS). \u003cem\u003eInt. J. Health Sci. (Qassim)\u003c/em\u003e. \u003cb\u003e5\u003c/b\u003e (2 Suppl 1), 27\u0026ndash;29 (2011).\u003c/span\u003e\u003c/li\u003e\u003cli\u003e\u003cspan\u003eKrittayaphong, R. et al. Correlation Between NT-pro BNP levels and left ventricular wall stress, sphericity index and extent of myocardial damage: a magnetic resonance imaging study. \u003cem\u003eJ. Card Fail.\u003c/em\u003e \u003cb\u003e14\u003c/b\u003e (8), 687\u0026ndash;694 (2008).\u003c/span\u003e\u003c/li\u003e\u003cli\u003e\u003cspan\u003eWeber, M. \u0026amp; Hamm, C. Role of B-type natriuretic peptide (BNP) and NT-proBNP in clinical routine. \u003cem\u003eHeart\u003c/em\u003e \u003cb\u003e92\u003c/b\u003e (6), 843\u0026ndash;849 (2006).\u003c/span\u003e\u003c/li\u003e\u003cli\u003e\u003cspan\u003eRiezebos, R. K., Ronner, E., Tijssen, J. G. P. \u0026amp; Laarman, G-J. NT-ProBNP serum levels reflect severity and extent of ischemia in patients admitted with non-ST-elevation acute coronary syndrome. \u003cem\u003eAcute Card Care\u003c/em\u003e. \u003cb\u003e8\u003c/b\u003e (1), 51\u0026ndash;57 (2006).\u003c/span\u003e\u003c/li\u003e\u003cli\u003e\u003cspan\u003eSarak, T. \u0026amp; Karadeniz, M. The relationship between serum NT-proBNP levels and severity of coronary artery disease assessed by SYNTAX score in patients with acute myocardial infarction. \u003cem\u003eTurk. J. Med. Sci.\u003c/em\u003e \u003cb\u003e49\u003c/b\u003e (5), 1366\u0026ndash;1373 (2019).\u003c/span\u003e\u003c/li\u003e\u003cli\u003e\u003cspan\u003eJering, K. S. et al. Prognostic Importance of NT-proBNP (N-Terminal Pro-B-Type Natriuretic Peptide) Following High-Risk Myocardial Infarction in the PARADISE-MI Trial. \u003cem\u003eCirc. Heart Fail.\u003c/em\u003e \u003cb\u003e16\u003c/b\u003e (5), e010259 (2023).\u003c/span\u003e\u003c/li\u003e\u003cli\u003e\u003cspan\u003eTakasu, S., Matsumoto, S., Kanto, Y., Kodama, S. \u0026amp; Iwadate, K. Postmortem urine concentration of N-terminal pro-brain natriuretic peptide in relation to the cause of death. \u003cem\u003eForensic Sci. Int.\u003c/em\u003e \u003cb\u003e306\u003c/b\u003e, 110079 (2020).\u003c/span\u003e\u003c/li\u003e\u003cli\u003e\u003cspan\u003eMichaud, K. et al. Evaluation of postmortem measurement of NT-proBNP as a marker for cardiac function. \u003cem\u003eInt. J. Legal Med.\u003c/em\u003e \u003cb\u003e122\u003c/b\u003e (5), 415\u0026ndash;420 (2008).\u003c/span\u003e\u003c/li\u003e\u003cli\u003e\u003cspan\u003eHarish, G. et al. Glutathione metabolism is modulated by postmortem interval, gender difference and agonal state in postmortem human brains. \u003cem\u003eNeurochem Int.\u003c/em\u003e \u003cb\u003e59\u003c/b\u003e (7), 1029\u0026ndash;1042 (2011).\u003c/span\u003e\u003c/li\u003e\u003cli\u003e\u003cspan\u003eSilva, A. R. T. et al. Repair of oxidative DNA damage, cell-cycle regulation and neuronal death may influence the clinical manifestation of Alzheimer's disease. \u003cem\u003ePLoS One\u003c/em\u003e. \u003cb\u003e9\u003c/b\u003e (6), e99897 (2014).\u003c/span\u003e\u003c/li\u003e\u003cli\u003e\u003cspan\u003eTettamanti, C., Hervet, T., Grabherr, S. \u0026amp; Palmiere, C. Elevation of NT-proBNP and cardiac troponins in sepsis-related deaths: a forensic perspective. \u003cem\u003eInt. J. Legal Med.\u003c/em\u003e \u003cb\u003e130\u003c/b\u003e (4), 1035\u0026ndash;1043 (2016).\u003c/span\u003e\u003c/li\u003e\u003cli\u003e\u003cspan\u003eFukuda, M. et al. The evaluation of oxidative DNA damage in children with brain damage using 8-hydroxydeoxyguanosine levels. \u003cem\u003eBrain Dev.\u003c/em\u003e \u003cb\u003e30\u003c/b\u003e (2), 131\u0026ndash;136 (2008).\u003c/span\u003e\u003c/li\u003e\u003cli\u003e\u003cspan\u003eRosato, E. et al. Forensic Biochemical Markers to Evaluate the Agonal Period: A Literature Review. \u003cem\u003eMolecules\u003c/em\u003e \u003cb\u003e26\u003c/b\u003e (11), 3259 (2021).\u003c/span\u003e\u003c/li\u003e\u003c/ol\u003e"}],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":true,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":false,"highlight":"","institution":"","isAcceptedByJournal":true,"isAuthorSuppliedPdf":false,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":false,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"scientific-reports","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":false,"externalIdentity":"scirep","sideBox":"Learn more about [Scientific Reports](http://www.nature.com/srep/)","snPcode":"","submissionUrl":"","title":"Scientific Reports","twitterHandle":"","acdcEnabled":true,"dfaEnabled":true,"editorialSystem":"stoa","reportingPortfolio":"Scientific Reports","inReviewEnabled":true,"inReviewRevisionsEnabled":true},"keywords":"Forensic pathology, 8-OHdG, Acute ischemic heart diseases, Postmortem diagnosis, NT-proBNP","lastPublishedDoi":"10.21203/rs.3.rs-7359354/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-7359354/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003eAcute ischemic heart disease (AIHD) is a major cause of sudden cardiac death, yet postmortem diagnosis is difficult, particularly in early-phase ischemic injury or decomposed bodies. We investigated the diagnostic value of two molecular biomarkers\u0026mdash;8-hydroxy-2\u0026prime;-deoxyguanosine (8-OHdG), indicating oxidative DNA damage, and N-terminal pro-brain natriuretic peptide (NT-proBNP), reflecting cardiac wall stress\u0026mdash;in 67 human forensic autopsy cases (33 AIHD, 34 non-AIHD). Immunohistochemistry revealed significantly greater and more diffuse nuclear 8-OHdG expression in AIHD cardiomyocytes compared with controls. Postmortem blood NT-proBNP levels were also significantly higher in AIHD cases (2389.1\u0026thinsp;\u0026plusmn;\u0026thinsp;499.6 pg/mL) than in non-AIHD cases (896.1\u0026thinsp;\u0026plusmn;\u0026thinsp;190.1 pg/mL). No significant correlations were found between 8-OHdG expression and NT-proBNP levels, contraction band necrosis, postmortem interval, age, or sex, suggesting that these markers reflect distinct pathological processes\u0026mdash;oxidative injury and hemodynamic stress. Both markers demonstrated postmortem stability, and their combined evaluation improved diagnostic confidence in cases with inconclusive histology. This dual-biomarker approach may enhance the accuracy of AIHD diagnosis in forensic practice and provide complementary insights into the molecular mechanisms underlying sudden cardiac death.\u003c/p\u003e","manuscriptTitle":"8-OHdG and NT-proBNP as complementary biomarkers in the postmortem diagnosis of acute ischemic heart disease","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2025-09-04 00:10:01","doi":"10.21203/rs.3.rs-7359354/v1","editorialEvents":[{"type":"communityComments","content":0},{"type":"decision","content":"Revision requested","date":"2025-10-16T04:13:59+00:00","index":"","fulltext":""},{"type":"editorInvitedReview","content":"","date":"2025-09-19T11:26:18+00:00","index":"hide","fulltext":""},{"type":"reviewerAgreed","content":"302263520484644967673383047025027946560","date":"2025-09-12T11:23:02+00:00","index":"hide","fulltext":""},{"type":"reviewerAgreed","content":"268269492182001208689081623611188522875","date":"2025-09-12T10:40:25+00:00","index":"hide","fulltext":""},{"type":"editorInvitedReview","content":"","date":"2025-09-03T05:03:32+00:00","index":"hide","fulltext":""},{"type":"reviewerAgreed","content":"267901111025894668600611067603188017817","date":"2025-08-30T00:10:30+00:00","index":"hide","fulltext":""},{"type":"reviewersInvited","content":"","date":"2025-08-27T10:19:15+00:00","index":"","fulltext":""},{"type":"editorInvited","content":"","date":"2025-08-14T13:43:31+00:00","index":"","fulltext":""},{"type":"editorAssigned","content":"","date":"2025-08-14T07:19:42+00:00","index":"","fulltext":""},{"type":"checksComplete","content":"","date":"2025-08-13T15:03:30+00:00","index":"","fulltext":""},{"type":"submitted","content":"Scientific Reports","date":"2025-08-12T22:35:28+00:00","index":"","fulltext":""}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"scientific-reports","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":false,"externalIdentity":"scirep","sideBox":"Learn more about [Scientific Reports](http://www.nature.com/srep/)","snPcode":"","submissionUrl":"","title":"Scientific Reports","twitterHandle":"","acdcEnabled":true,"dfaEnabled":true,"editorialSystem":"stoa","reportingPortfolio":"Scientific Reports","inReviewEnabled":true,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"d6094cff-b504-4a4a-bdd7-d4376844be0e","owner":[],"postedDate":"September 4th, 2025","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"published-in-journal","subjectAreas":[{"id":53926541,"name":"Health sciences/Biomarkers"},{"id":53926542,"name":"Health sciences/Cardiology"},{"id":53926543,"name":"Health sciences/Diseases"},{"id":53926544,"name":"Health sciences/Medical research"}],"tags":[],"updatedAt":"2026-02-23T16:08:24+00:00","versionOfRecord":{"articleIdentity":"rs-7359354","link":"https://doi.org/10.1038/s41598-026-38797-w","journal":{"identity":"scientific-reports","isVorOnly":false,"title":"Scientific Reports"},"publishedOn":"2026-02-19 15:59:32","publishedOnDateReadable":"February 19th, 2026"},"versionCreatedAt":"2025-09-04 00:10:01","video":"","vorDoi":"10.1038/s41598-026-38797-w","vorDoiUrl":"https://doi.org/10.1038/s41598-026-38797-w","workflowStages":[]},"version":"v1","identity":"rs-7359354","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-7359354","identity":"rs-7359354","version":["v1"]},"buildId":"8U1c8b4HqxoKbykW_rLl7","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}