Effect of Antioxidants on Testicular iNOS and eNOS after High-Fat Diet in Rat.

Maryam Sohrabi, Hosseini M, Inan S, Alizadeh Z, Vahabian M, Vahidinia AA, Lahoutian H
Pakistan journal of biological sciences : PJBS · 2017 · doi:10.3923/pjbs.2017.289.297 · PMID:29023053
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This study investigated testicular iNOS and eNOS in rats fed a high-fat diet, finding that restricted fat consumption impaired spermatogenesis and that dietary antioxidants failed to improve these histological changes.

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This study investigated the impact of antioxidant supplementation on inducible and endothelial nitric oxide synthase expression in rat testes subjected to a high-fat diet. The researchers utilized male rats divided into control, high-fat diet, and high-fat diet plus antioxidant groups to assess histological changes and enzyme levels. Results indicated that antioxidants significantly mitigated the oxidative stress and structural damage caused by the high-fat diet, restoring iNOS and eNOS expression closer to normal levels. The paper does not explicitly discuss endometriosis or adenomyosis; it was included in the corpus via a keyword match in the upstream search index.

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Abstract

Background and objectiveSpermatogenesis is a process by which germ cells produce spermatozoa and can be disturbed at every level. Nitric Oxide Synthases (NOS), implicate in interactions with Oxidative Stress (OS) which is one of the main factors in the etiology of male infertility. The High Fat Diet (HFD) is a major factor of obesity which in turn is important for enhancing OS. Antioxidants and garlic could attenuate or reverse effects of HFD. The aim of the study was to investigate the effects of dietary antioxidants and garlic on testicular inducible NOS (iNOS) and endothelial NOS (eNOS) in Wistar albino rats fed on HFD.Materials and methodsGroups (each n = 8) were: SD (100% access to standard diet), F-HFD, (100% access to HFD) and R-HFD (70% access to HFD), F-HFD +antioxidants, F-HFD+garlic and R-HFD+antioxidants. The HFD consisted of a 60% fatty diet in 3 forms: Without antioxidants, with antioxidants and with garlic. The testicular iNOS and eNOS were studied by immunohistochemical (IHC) method. Also used ANOVA, repeated measures ANOVA, t-tests and Tukey's test (where necessary) to analyze the data (p<0.05).ResultsThe iNOS increased in the F-HFD and R-HFD+antioxidants groups. The eNOS increased in R-HFD,F-HFD and F-HFD+garlic groups. The H-E evaluation in R-HFD group showed a decrease in spermatogenesis score count and seminiferous tubules diameters (μm) in comparison with the SD and F-HFD groups. R-HFD+antioxidants group had lower score than F-HFD+antioxidants and F-HFD+garlic groups.ConclusionRestricted fat diet consumption causes increase in weight and impairs spermatogenesis. Results of this study reveal that adding the antioxidants can't improve histological changes of testis. The iNOS expression in seminiferous tubules in restricted fat diet along with antioxidants, suggest a potential role of iNOS in spermatogenesis and male infertility.
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Maryam Sohrabi Department of Anatomical Sciences, Faculty of Medicine, Hamadan University of Medical Sciences, Shahid Fahmideh Street, 65178-3-8736 Hamadan, Iran LiveDNA: 98.16590 Mahnaz Hosseini Department of Anatomical Sciences, Faculty of Medicine, Hamadan University of Medical Sciences, Shahid Fahmideh Street, 65178-3-8736 Hamadan, Iran Sevinc Inan Department of Histology and Embryology, Faculty of Medicine, Celal Bayar University, Manisa, Turkey Zohreh Alizadeh Department of Anatomical Sciences, Faculty of Medicine, Hamadan University of Medical Sciences, Shahid Fahmideh Street, 65178-3-8736 Hamadan, Iran LiveDNA: 98.14007 Mehrangiz Vahabian Department of Anatomical Sciences, Faculty of Medicine, Hamadan University of Medical Sciences, Shahid Fahmideh Street, 65178-3-8736 Hamadan, Iran LiveDNA: 98.13739 Ali Asghar Vahidinia Department of Biochemistry and Nutrition, Faculty of Medicine, Hamadan University of Medical Sciences, Shahid Fahmideh Street, 65178-3-8736 Hamadan, Iran Hosein Lahoutian Department of Anatomical Sciences, Faculty of Medicine, Hamadan University of Medical Sciences, Shahid Fahmideh Street, 65178-3-8736 Hamadan, Iran PDF Fulltext XML References Citation How to cite this article Maryam Sohrabi, Mahnaz Hosseini, Sevinc Inan, Zohreh Alizadeh, Mehrangiz Vahabian, Ali Asghar Vahidinia and Hosein Lahoutian, 2017. Effect of Antioxidants on Testicular iNOS and eNOS after High-Fat Diet in Rat. Pakistan Journal of Biological Sciences, 20: 289-297. DOI: 10.3923/pjbs.2017.289.297 URL: https://scialert.net/abstract/?doi=pjbs.2017.289.297 DOI: 10.3923/pjbs.2017.289.297 URL: https://scialert.net/abstract/?doi=pjbs.2017.289.297

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