Paleoclimate variations in Western Amazon based on Lago Airo (Brazil) diatoms from the last 13,300 years cal BP

Paleoclimate variations in Western Amazon based on Lago Airo (Brazil) diatoms from the last 13,300 years cal BP | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Paleoclimate variations in Western Amazon based on Lago Airo (Brazil) diatoms from the last 13,300 years cal BP Letícia Rizzetti Patrocínio, João Cláudio Cerqueira Viana, Doriedson Ferreira Gomes, and 8 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-7687264/v1 This work is licensed under a CC BY 4.0 License Status: Under Review Version 1 posted 12 You are reading this latest preprint version Abstract The study of paleolimnological and paleoclimatic variations in the Amazon from the last 13,300 cal yr BP is essential for interpreting the climatic and environmental history of this region, as it is one of the largest and most diverse biomes on the planet. Diatom assembly (density and relative abundance), sedimentological, bulk, and isotopic organic geochemistry analyses were obtained from a 146 cm-long core collected in a marginal lake nowadays disconnected from Rio Negro (Lago Airo; 00º19’37.225’’S, 66º08’33.266’’W). Seven organic matter samples were dated by using AMS radiocarbon determination. The 73 species found mostly comprised acidophilic species. We discerned four main phases: Phase IV, from ca. 13,400 cal yr BP to ca 11,800 cal yr BP: diatoms density was relatively low (i.e., ranging from 88 to 37.5 x 10³ valves/g) with mainly periphytic taxa. Sediments are comprised of coarser grains (sand > 99%). Geochemical data shows a TOC average of 2.2%, the C/N ratio is higher (average = 52.1), and chlorophyll derivatives are lower (average = 5.6 SPDU). 15N values show a source related to nitrogen atmospheric fixation metabolism, probably due to the extremely oligotrophic nature of the system. Our results suggest that Phase IV represented the Lago Airo as a lotic-like ecosystem with connections with the Rio Negro, characterized by high energy and thereby low diatom sedimentation. Phase III: 11800 to 8800 cal yr BP: The diatom community kept characteristics of low density with the domain of Eunotia spp (E. hirudo, E. bilunaris, and E. floweri). This phase exhibits a significant increase in clay content of around an order of magnitude, rising from 0.256% in phase IV to 2.05% in phase III. Although the absolute values are still low, they still constitute a sandy-textured sediment. TOC values increased compared to the previous phase, reaching 2.39%, with an increase in the C/N ratio denoting the transport of detrital organic matter from the basins. Phase II: 8800 to 4100 cal yr BP: The diatom community keep characteristics of phase III. This phase exhibits a decrease in clay content to 0.299% with a progressive increase in TOC, compared to the previous phase, reaching 3.47%. This increase in C/N ratio indicates the transport of detrital organic matter from the basins. On the onset of phase I, the Rio Negro became disconnected from Lago Airo, which is suggested by the predominance of silt, associated with low hydrodynamics, and an increase in diatom valve density. Phase I: 4100 to actual: This phase shows an increased diatom valves density (between 30.9 x 104 and 14.1 x 106), composed mainly by planktonic taxa. Silt became dominant (average = 59.1; clay = 10.2) simultaneously with. Higher silt contribution with a pronounced decrease in C/N ratios (average = 34.9) is indicative of declining influence of the Rio Negro over Lago Airo and an increase in autochthonous production, represented by the increase in TOC, changing from 3.47 in phase II to 25.0%, and chlorophyll derivatives (average = 12.7 SPDU). Our results suggest an increase in the mean water level in Lago Airo (characterized by higher valves/g, planktonic taxa dominance - Aulacoseira spp. - A. distans and A. granulata, mainly, and δ15N values increase), which was probably the result of higher precipitation regimes in the South American Monsoon System. Pleistocene Holocene Paleolimnology Eunotia spp Aulacoseira spp geochemistry Rio Negro Figures Figure 1 Figure 2 Figure 3 Figure 4 Figure 5 Figure 6 Figure 7 1. Introduction The Amazon region has substantial participation on heat distribution and humidity release from the Tropics, influencing in climate and precipitation rates from global to local scales (Werth and Avissar 2002 ). The main climate influence of the Amazon is the presence of moisture from the Amazon Forest from eastern Brazil (e.g. South American Summer Monsoon - SASM) and the displacement of the Inter-Tropical Convergence Zone - ITCZ (Cruz et al. 2009 ). In respect of distinct climatic variabilities at different time scales, wet and dry conditions at the Amazon basin are related throughout the Holocene, and these periods are controversial (Silva et al. 2018 ). The ITCZ is a band of clouds induced by low-level convergence of Tradewinds over equatorial oceans, that promotes a minimum in atmospheric pressure, a low mixed-layer depth, a maximum of Sea Surface Temperature (SST), and deep convective rainfall (Garreaud et al. 2008 ). The Amazon River basin and Amazon Forest contribute to climate dynamics on Tropical South America being affected by complex dynamics teleconnections (Potter et al. 2004 ), and astronomical forcing (e.g. insolation changes explained by Milankovitch cycles) (Silva Dias et al. 2009 ). The Rio Negro (Amazon River basin) is the largest black water river in the world, with a basin area of around 700,000 km2 passing through Colombia, Venezuela, and Brazil (Latrubesse and Franzinelli, 2005 ). According to these authors its formation is dated back to the Late Pleistocene and Holocene, associated with activity during the last glacial in the Amazon Basin. However, paleoecological studies in remote areas like the upper part of Rio Negro are scarce (Rodríguez-Zorro et al. 2017 ). The analysis of three sediment records in three lakes of the Morro dos Seis Lagos stream in the upper Rio Negro showed no evidence of changes in vegetation physiognomy during the late Pleistocene and Holocene (Colinvaux et al. 1996 , Bush et al. 2004 ), rather than significant evidence of changes in lake levels (Bush et al. 2004 , Cordeiro et al. 2011 ). Changes in atmospheric patterns between the end of glaciation and the beginning of the warmer conditions of the Holocene were observed through changes in mercury fluxes in the Lagoa da Pata (Barbosa et al. 2004 ). The aim of this study was to infer paleolimnological dynamics and paleoclimate conditions at western Amazon, based on Lago Airo (Rio Negro basin) sediment core and major studies in the area. This research was based on a multiproxy approach that included geochemistry, multiple sediment variables analyses and diatom assemblage. Our results assist in understanding paleolimnological and climate variations insights throughout the Holocene and Pleistocene/Holocene transition of the Amazon basin, from registers of a lake located at extreme northwestern Brazil. 2. Site Description Lago Airo is a floodplain lake in Rio Negro basin (0°19’37.225” S, 66°8’33.266” W), Municipality of São Gabriel da Cachoeira city, Amazonas State (Fig. 1 ). Rio Negro is the largest tributary of Amazon River, considered the fifth largest river in the world (Franzinelli and Igreja 2002 ). The Negro River the most important black water river and the second largest tributary of the Amazon River drains an area of 696,810 km 2 , with a discharge of about 28,400 m − 3 s − 1 and provides an average of 8 Mt. yr − 1 of suspended sediment flux. Lago Airo has approximately three meters of depth in the collecting date, maximum width of 300 m, pH around 3.5, and nowadays has no connection with Rio Negro. Located on equatorial region, in the Amazon Forest biome, with a hot and humid climate. The annual rainfall average on São Gabriel da Cachoeira city area is 2,853 mm (Ribeiro et al. 1996 ). The vegetation around the lake is Campinarana , which is composed by 12 m arboreum strata distributed continuously with scarce open fields. The sunlight reaches the soil. It is a vegetation which occurs on hydromorphic Podzolic soils, with hydromorphic quartz sands on the alluvial plain of the Negro River. The campinaranas occur on flat and flooded areas around the lake, and present quite varied physiognomy as observed in the region with campestral and forest formations. Vegetation is characterized by soil rich in organic matter, and slow decomposition due to low pH values and large aluminum concentration (Martins and Matthes1978). 3. Material and Methods 3.1. Core Sampling and Radiocarbon chronology. Sediments from Lago Airo were collected in 2015 using a vibro-coring system equipped with aluminum tubes measuring 7.6 cm in diameter. The sampling platform consisted of two rubber boats, which facilitated the retrieval of the AIRO 12/01 core, measuring 146 cm in length (0°19'37.225" S, 66°8'33.266" W). The description of the sedimentary profile was carried out as soon as the core was opened, and its lithology was described with the help of a Munsell Colour Chart. The sediment core was sliced into 1.0 cm sections which were stored in plastic bags. Seven calibrated 14 C dates of bulk organic matter based the age-depth model. Measurements of 14 C activity were made by Acceleratory Mass Spectrometry (AMS) at the Radiocarbon Dating Laboratory Beta Analytic, located in Miami, Florida, Laboratoire de Mesure du Carbone 14 (Table 1 ). The program Calib 7.1 (Stuiver et. al. 2018 ) was used for calibration due to the natural variations of 14C (available at: http://calib.org/calib/ ), and dates were converted to cal BP. To model the age-depth relationship, the R package “Bacon” (Blaauw and Christen, 2011) was used, which adopts a Bayesian approach to establish a robust chronological sequence. This model integrates available radiometric dates and, through the IntCal 13 calibration curve, converts these dates into calibrated ages (cal yr BP), providing an accurate temporal reconstruction of the observed sedimentary variations along the profile. 3.2. Grain-size Distributions A laser particle analyzer (CILAS Particle Analyzer® 1064) was used to measure the grain-size distribution of the mineral fraction (particles between 0.04 and 500 µm) after the organic matter and carbonate destruction with H 2 O 2 and HCl, respectively, and it is dispersing in a Na 4 P 2 O 7 solution and sonifcation. The program GRADISTAT VERSION 4.0, was used to calculate the granulometric fraction (Blott 2000 ). 3.3. Bulk and isotopic organic geochemistry The organic geochemical measurements made included Total Organic Carbon (TOC), Total Nitrogen (TN), δ 13 C (‰) and δ 15 N (‰). Samples were analyzed for TOC and stable isotopic (δ 13 C and δ 15 N) content to CHN analyzer (PDZ Europa ANCA-GSL) connected to an Isotope Ratio Mass Spectrometer (IRMS) PDZ Europa 20–20 (Sercon Ltd., Cheshire, UK) at UC Davis Stable Isotope Facility (SIF), Department of Plant Sciences, CA, USA. Samples were treated with 0.5 N HCl to remove carbonate prior to isotopic measurements. It was analyzed lithology, granulometry, total organic carbon (TOC), C:N ratio, δ 15 N and δ 13 C. The analysis of chlorophyll derivatives in sediment samples was performed according to the method of Sanger and Gorham ( 1972 ), adapted by Cordeiro et al. ( 1997 ), (Chlorophyll derivatives were extracted with 90% acetone and measured at 667 nm in Perkin Elmer spectrometer). The results were expressed in arbitrary units as absorbance per gram organic matter, where one unit (SPDU) is equal to an absorbance of 1.0 in a 10 cm cell, when dissolved in 100 ml of solvent. 3.4. Diatom assemblages 3.4.1. Samples oxidation and slide preparation Sediment samples oxidation followed the standard method of Battarbee et al. ( 2001 ). About 1 g of humid sediment and 30 ml of H 2 O 2 at 37% were add to a beaker. This solution remained for 24h in hot plate (50°C ± 5) until all organic matter was removed. Samples were washed in distilled water until complete dilution of H 2 O 2 . Slides were prepared by dropping 1 ml of diatom suspension on a coverslip. The diatoms were allowed to settle and water to evaporate in a hot plate (50°C ± 5). When dry, slides were mounted using a resin Naphrax® (index = 1.74). 3.4.2. Identification and Counting Diatoms were counted (500 valves per sample) in each 1 (one) cm depth intervals from 0 to 34 cm (except 5–6 cm and 29–30 cm due to lack of samples). After that, analyses were carried out each 2 cm intervals, totalizing 86 samples of sediment from Lago Airo. From 34 cm to 145 cm it was set a pattern of 4 transects per slide due to very few amount of diatoms in slides. Diatoms in each sample were identified to the lowest possible taxonomic level along random transects using an Olympus CX31 light microscope at 1000x magnification. Valves were counted if more than one-third of their original structure was intact, including cases where the valve center was present or when specific morphological features characteristic of the species were observed, to minimize overestimation (Battarbee et al., 2001 ). Identification of species was conducted using mainly the following references keys: Patrick and Reimer ( 1975 ), Lange-Bertalot ( 1966 , 2007 ), Metzeltin and Lange-Bertalot ( 1998 ), DeNicola ( 2000 ) and Ferrari et al. ( 2007 ). 3.4.3. Diatom Analysis The species’ relative abundance (%) and species’ density (valves/g) represented the diatom assemblage composition. Absolute abundance value of each species per slides represented the taxa relative abundances. The cut-off value for the species selected as the most representative within the community was 1% of relative abundance at least in 3 depths of the sediment samples. These species’ relative abundance was classified as benthic or planktonic according to autecological live characters. The diatom density per layer sampled was calculated from the total of valves counted per slide corrected by samples bulk density. The ratios of planktonic to benthic diatoms were calculated using the formula: P:B ratio = planktonic taxa / benthic taxa. A broken-stick model was used (Bennett 1996 ) to determine the number of diatom zones. 4. Results Through cluster analyses that incorporate the total diatom assemblage distribution, four primary phases have been established. Accordingly, the subsequent parameter description will encompass these subdivisions (Fig. 2 ). 4.1. Lithology, Chronology and Bulk Density The Airo 12/01 sediment core has a length of 1.46 meters. According to the lithological description provided in Table 3, the presence of three main units and five sub-units can be observed. In lithological unit III, which spans from 146 to 91 cm, three sub-units can be identified. The lithological description indicates the occurrence of sandier sediment within this unit. Sub-units IIIc and IIIa exhibited a brown color pattern (referred to as "brown" according to the "Munsell Soil Color Chart"). Lithological unit II consists of two sub-units (IIa and IIb) and spans the interval from 90 to 30 cm. Like lithological unit III, the presence of sandy sediment was noted in unit II. The color pattern observed during the lithological description ranged from dark reddish-brown in sub-unit IIb to very dark gray in sub-unit IIa. The first lithological unit represents the uppermost 29 centimeters of the sediment core. A noticeable change in grain size occurs between this unit and unit II, where finer sandy sediment is observed in unit I. The color pattern observed in this lithological unit was black, as per the Munsell Soil Color Chart. Table 1 General description of the lithological units of Airo 12/01 core. Lithological Units Sub-units Section (cm) Sedimentological characterisation Colour Standard Colour Code I 29 − 0 3000-Actual Silt Black 5YR 2.5/1 II IIa 52 − 29 6910 − 3000 Sand with plant fragments Very Dark Grey 5YR 3.1 IIb 90 − 52 11630 − 6910 Sand Dark Reddish Brown 5YR 3.2 III IIIa 101 − 90 12440 − 11630 Sand Brown 10YR 4.2 IIIb 112 − 101 12970 − 12440 Sand with plant fragments Dark Grayish Brown 10YR 3.2 IIIc 146 − 112 13360 − 12970 Sand Brown 10YR 4.2 Three radiocarbon dates on total organic matter and four datations from leaf samples were obtained by Accelerator Mass Spectrometry (AMS) in the Airo 12/01 core. Table 2 Description of radiocarbon ages on AIRO 12/01 core by AMS Method Depth interval (cm) Datation code Measured sample Conventional 14 C age (years BP) 2-sigma intercept age (cal yr BP) Age range 2-sigma 15 cm Beta 388870¹ Bulk organic matter on Sediment 1170 ± 30 1065 986–1179 28 cm SacA 50900² Bulk organic matter on sediment 1575 ± 30 1410 1399–1537 50 cm Beta 388871¹ Plant material 4930 ± 30 5650 5600–5719 70 cm Beta 388872¹ Plant material 8550 ± 30 9520 9493–9547 78 cm 18OS/0403³ Bulk organic matter on sediment 8420 ± 30 9490 9334–9519 116 cm Beta 388873¹ Plant material 11430 ± 30 13200 13255– 13300 143 cm Beta 388874¹ Plant material 11270 ± 30 13130 13058–13212 ¹Radiocarbon Dating Laboratory Beta Analytic; ²Laboratoire de Mesure du Carbone 14; and ³ International Chemical Analysis Inc. The radiocarbon ages from the Airo 12/01 core indicate sedimentation for the last 13,300 years cal BP (Table 2 , Fig. 3 ), corresponding to the Holocene, including the Pleistocene/Holocene transition. Figure 3 : Lithological profile, calibrated radiocarbon ages, sedimentation rate, and stratigraphic zones from Lago Airo. The lithology column shows sediment textures and colors with Munsell color notations. Radiocarbon ages (cal yr BP) are plotted against depth (cm) alongside the calibrated age model (solid red line) with confidence intervals (dashed gray lines). Sedimentation rates (cm/yr) are displayed on the right. Four stratigraphic zones (I–IV) are indicated based on changes in sedimentological characteristics and age-depth relationships. 4.2. Grain-size distribution An average percentage of sand-size particles was 79.3 ± 30.4% cm with 17.4 ± 24.4% of silt and just 3.3 ± 12.9% of clay. Considering the coefficient of variation among particle size classes, it is evident that the clay class exhibits the highest variability among the distributions, With a variation coefficient of 393%. Following this, the silt class shows a variability of 140%, and lastly, the sand class displays the least variability at 38.3%. Phase IV: 13,400 to 11,800 cal yr BP, 146 cm to 93 cm. In this phase, the sediment was predominantly composed of coarser grains, with sand constituting over 99% of the composition. The distribution of sand displayed low variability (variation coefficient less than 1%). The influx of sand intoLago Airo demonstrated uniformity throughout the designated period, suggesting a phase characterized by low variability and high energy, likely associated with a substantial intensification in the hydrological cycle of the Rio Negro. This period marks the transition between the Pleistocene and Holocene epochs and is globally recognized for its cold conditions, particularly the Younger Dryas, characterized by a rapid and drastic cooling of the climate, especially in the Northern Hemisphere (Rasmussen et al. 2006 ). Phase III: 11,800 to 8,800 cal yr BP, 92 to 68 cm . This phase shows a significant increase in clays and silt content in sediment and a great variability of sand. The transition from a period characterized by significant transport competence related to extreme climatic events shifts to a phase of substantial variability, as evidenced by a coefficient of variation that was previously less than 1% and has now increased to nearly 30% in terms of sand variability. This aspect indicates a period of intense changes in the lacustrine environment, alternating between extreme events and phases of substantial stability in the lacustrine system, accompanied by significant clay deposition, which was 0.285% in phase 4, increasing to a value of 2% in this phase. In Phase II: 8,800-4,100 cal yr BP, 67 to 38 cm. The sand sediments show an increase compared to the preceding phase and present a sediment rich in sand with average values of 97.9 ± 4.65%. Phase II is characterized by average sand values like Phase IV; however, it exhibits greater variability compared to phase IV, with a variation coefficient of 4.75% against 0.18% in Phase IV. Thus, during the mid-Holocene, a climate shift towards drier conditions in relation to precedent phase is observed, marked by extreme events of sandy sedimentation. Phase I: 4,100 to actual. Silt became dominant. At the onset of phase I, the Rio Negro became disconnected from Lago Airo, which is suggested by the predominance of silt, associated with low hydrodynamics, and an increase in diatom valve density. 4.3. Bulk and stable isotopic organic geochemistry The total organic carbon (TOC) content, with an average of 7.89 ± 11.7% for the entire profile, varied from 0.4% at 13,130 cal yr BP (Phase IV) to 41.9% at 780 cal yr BP (Phase 1). The TOC values exhibit increasing mean values across the phases, with concentrations of 1.41% in Phase IV, 2.39% in Phase III, and 3.47% in Phase II. However, a substantial increase is evident in Phase I, with mean values reaching 28%, indicating the consolidation of a lentic environment. The C/N ratio was greater than 20 throughout Airo 12/01 core with an average value of 47.8 ± 12.9 indicating a huge influence of terrestrial organic matter, with a strong degradation component. The C/N ratio varying between 4 and 10 (~ 8) commonly suggests an algae source, whereas C/N ratios greater than 20 are attributed to an origin of macrophytic margin vegetation or terrestrial plant source (Meyers 2003 ). A trend towards an increase in the C/N ratio is observed from Phases IV to II, ranging between 49.3 ± 9.58 during Phase IV, 52.6 ± 7.08 during Phase III, and 58.1 ± 5.70 during Phase II, likely indicating a period when autochthonous influence was reduced because of a regional dry phase (Nascimento et al. 2021 ). An abrupt decrease in the C/N ratio values is observed during Phase I, with an average of 35.5 ± 7.67. This denotes a significant increase in autochthonous influence, as evidenced by high values of sedimentary chlorophyll. In Phase IV (ca. 13,400 to 11,800 cal yr BP), geochemical data show a TOC average of 2.2%, high C/N ratios (average = 52.1), and low chlorophyll derivatives (average = 5.6 SPDU). δ15N values suggest a source related to atmospheric nitrogen fixation, likely due to the extremely oligotrophic nature of the system. These results suggest that Phase IV represented Lago Airo as a lotic-like ecosystem with strong connections to the Rio Negro. The δ15N values approaching 0‰ support the presence of predominantly terrestrial organic material. The loss of nitrogen induces that this organic matter is poor in Nitrogen favoring the biological process of nitrogen fixation (references) attested by δ15N values near 0‰. Negative values, particularly evident in Phase IV, suggest significant decomposition processes of the organic material, likely due to prolonged exposure in the podzolized soils prevalent in the Rio Negro basin. In Phase III (11,800 to 8,800 cal yr BP) , exhibiting substantial variability. T here was a significant increase in clay content, rising from 0.256% in Phase IV to 2.05%, though the sediments remained sandy-textured overall. TOC values increased slightly to 2.39%, and higher C/N ratios indicated the transport of detrital organic matter from the surrounding basin. Phase II (8,800 to 4,100 cal yr BP) there was a decrease in clay content (to 0.299%) alongside a progressive increase in TOC, reaching 3.47%. During this phase Chlorophyll derivatives values around 5,501 to 20.2 SPDU, showed lake-like conditions as well as the information given by C/N (Fig. 3 ). Higher C/N ratios continued to reflect detrital organic matter input. This phase marks the onset of the disconnection between Lago Airo and the Rio Negro and aligns with broader mid-Holocene dry conditions, with decreasing lake levels observed across Amazonia. Lago Airo’s ecosystem showed evidence of reduced fluvial connectivity and higher external organic input, consistent with high C/N ratios, and reflects progressive ecological isolation. Phase I (4,100 cal yr BP to present) Sediments transitioned to being predominantly silt (average = 59.1%) with an increase in clay (average = 10.2%). This phase also saw a pronounced increase in autochthonous production, as evidenced by rising TOC values (up to 25.0%) and chlorophyll derivatives (average = 12.7 SPDU). These changes suggest an increase in mean water level, likely driven by higher precipitation associated with the South American Monsoon System. The shift to finer sediments, coupled with increased diatom density and autochthonous production, highlights the establishment of a lentic system. This phase represents the wettest conditions in Amazonia over the past millennia, favoring ecosystem stabilization, higher productivity, and organic matter accumulation in Lago Airo. 4.4. Diatoms Assemblage It was identified 73 taxa. The 10 most frequent were Eunotia hirudo Metzeltin and Lange-Bertalot (mean 20.6 ± 21.4%), Aulacoseira distans (Ehrenberg) Simonsen (12.3 ± 14.3%), Eunotia didyma Grunow var. didyma (7.6 ± 12.6%), Aulacoseira sp. 3 (6.4 ± 12.1%), Aulacoseira cf. calypsi Tremarin, Torgan et Ludwig (3.4% ± 11.1%), Eunotia ventriosa Patrick (3.2%), Eunotia bilunaris (Ehenberg) Schaarschmidt (3.1 ± 4.7%), Eunotia gibbosa Grunow in Van Heurck (2.9% ± 4.4%), Aulacoseira granulata (Ehrenberg ) Simonsen (2.8 ± 3.7%), Eunotia floweri Metzeltin and Lange-Bertalot (2.7 ± 6.2%). Figure 4 shows diatom diagram. The diatoms density attained a peak (37,542 valves/g) around 10k cal. years BP (79 cm). Eunotia hirudo, Eunotia bilunaris , and Eunotia floweri were highly abundant during phases PIV, PIII, and PII. Phase II was marked with the disappearance of Aulacoseira cf. calypsi from records and a small decrease of Eunotia hirudo and Eunotia bilunaris . Phase I recorded the highest diatom densities, with average of 5.698,183 valves/g. Planktonic species dominated this phase with Aulacoseira distans and A. granulata , while A. cf. calypsi reappeared on record and Eunotia floweri abundance decreased. Phase IV, from ca. 13,360 (145 cm) to 11,800 cal yr BP (92 cm). Diatoms valves density was relatively low (i.e., average = 1,684 valves/g ± 1,800; CV = 107%; minimum of 88 and maximum of 7,337) with mainly periphytic taxa. Sediments was dominated by coarser sediments (sand represents 99,7%). Geochemical data shows a TOC average of 1.4% ± 0,8; CV = 56,1%; minimum of 0.40% and maximum of 3.54%. C/N ratio presents high values (average = 50.3 ± 10.1; CV = 20.1%; minimum of 26.1 and maximum of 71.6., Chlorophyll derivatives are presents low values with average of 2.93 ± 1.6; CV = 54,3%; minimum of 0,76 SPDU and maximum of 6.12 SPDU. 15 N values show a source related to a nitrogen atmospheric fixation metabolism, probably due to the extremely oligotrophic nature of the system. Our results suggest that Phase IV represented the Lago Airo as a lotic-like ecosystem with connections with the Rio Negro, characterized by high energy and thereby low diatom sedimentation. Phase III from ca. 11,800 (145 cm) – 8,800 cal yr BP (68 cm). Diatoms valves density was relatively low (i.e., average = 5,589 valves/g ± 9,455; CV = 107%; minimum of 0 and maximum of 37,543 valves/g) with mainly periphytic taxa. This phase was dominated by coarse sediments (sand represents 93,3%) and showed a progressive increase in TOC compared to the previous phase, reaching an average value of 2.39%, with a variation coefficient around 52.4%; minimum of 0,7 and maximum of 5.3%. In this phase was observed an increase in the C/N ratio denoted the increase transport of detrital organic matter from the basins. The C/N ratio presents high values of 54.6 ± 7.1 and a variation coefficient with low variability, around 13,5%; a minimum of 33.1 and a maximum of 66.6. The increase in the C/N ratio denotes the transport of detrital organic matter from the basins. Chlorophyll derivatives present low values with an average of 5.86 ± 2.00 SPDU; CV = 26,6%; minimum of 2.14 SPDU and maximum of 11.9 SPDU. Phase II: 8,800 (67 cm) to 4,100 (38 cm) cal yr BP . Diatoms valves density decrease in relation to the precedent phase (i.e., average = 1,454 valves/g ± 1,383; CV = 95%; minimum of 0 and maximum of 4,742 valves/g) with mainly periphytic taxa. Sediments was also dominated by coarse sediments (sand represent 97,9%). Geochemical data shows a TOC average of 3.47% ± 1.7%; CV = 49.7%; minimum of 1,36 and maximum of 5,3. The C/N ratio presents high values (average = 58,1 ± 5,7; CV = 9,81%; minimum of 48,6 and maximum of 70.8), and chlorophyll derivatives presents low values (average = 5,86 ± 3.74 SPDU; CV = 63,9%; minimum of 2,1 SPDU and maximum of 11,9 SPDU). Phase I: From ca. 4,100 cal yr BP (37 cm) to actual. Here we saw a change in diatom structure, density, and composition, marking a transition from a lotic system to a lentic one. Diatom density increased (i.e., average = 4.69 x10 6 valves/g ± 4.15; CV = 88%; minimum of 2.01 x10 3 valves/g and maximum of 14.1 x10 6 valves/g). These diatom communities were primarily composed of planktonic taxa. After approximately 4,100 cal yr BP there was a shift towards silt dominance in the sediment composition, with an average of 65.7 ± 30.4% (coefficient of variation = 46.3%), ranging from a minimum of 0.6% to a maximum of 93.0%, followed by clay = 11,1 ± 6,72%; CV = 60,1%; minimum of 0 and maximum of 20,3). The heightened contribution of silt and a noticeable decrease in C/N ratios, averaging 34.9 ± 7.7 (coefficient of variation = 21.9%), suggest a diminishing influence of the Rio Negro on Lago Airo and an increase in autochthonous production. This is evidenced by the rise in total organic carbon (TOC) content, which increased from 3.4% in Phase II to 25.0%. A substantial increase in chlorophyll derivative reaching was simultaneous reinforces the formation of a lacustrine environment with reduced influence from the Rio Negro, as we can see on diatom data. The planktonic-benthic ratio considered the mean relative abundance of the most abundant and significant species. All planktonic species were Aulacoseira spp. and the benthics were Eunotia spp. The P:B ratio also maintained the characteristic of two main phases formed by phases IV, III and II which is characterized by diatom low density and greater representation of Eunotia spp as opposed to phase I, which presents a high density of diatoms and the predominance of Aulacoseira spp. A direct relationship can be observed among the alterations in the structure ofLago Airo diatom community, as represented by the first factor in the principal component analysis and their abundance, which is assessed based on cell concentration in the sediment. Factor 1 serves as a principal component considering all species, indicating a transition from a benthic to a planktonic community. These changes in the diatom community precede modifications in diatom biomass, primarily resulting from the establishment of a more productive lentic environment. The abrupt shift between basal phases PIV, PIII and PII and phase 1 appears to be primarily preceded by a transformation in community structure, particularly evident from 4100 cal yr BP. Substantial shifts in diatom biomass become noticeable from 3660 cal yr BP onward. 5. Discussion 5.1. Paleolimnological interpretations Lago Airo records showed consequences of climate change on the Rio Negro basin and reveals a clear separation between the earlier high-energy, lotic system and the more recent low-energy, lentic conditions (Figs. 1 and 2 supplementary materials), reflecting high amplitude of hydrological and climatic changes in Lago Airo. The paleoclimate evidence is discussed for the Younger Dryas, Late Holocene and Early-Mid Holocene. The Airo 12/01 core first phase coincides with the Bølling-Allerød (15 − 13 ka yrs) end, a warm period of the high latitude North Atlantic region. Its formation coincides with the significant increase in the level of Lagoa da Pata recorded on the LPT V core mainly after 14,500 cal yr BP (Cordeiro et al. 2011 ). Since about 14,000 to 4,000 cal yr BP , the Rio Negro has behaved as a progradational system, infilling in downstream series a sequence of structurally controlled sedimentary basins or ‘compartments,’ creating alluvial floodplains and associated anabranching channel systems (Latrubesse and Franzinelli 2005 ). The Lago Airo seems to be part of this dynamic. About 750 km northeast of Lago Airo, in a region now dominated by cerrado vegetation (Brazilian classification), an integrative study of sediment cores from three lakes (Beltran et al. 2023 ) indicated savannah type ecosystems that have remained stable over the entire Holocene period, with minimal changes in vegetation. Lake sediment records indicate sensitivity to local water table fluctuations. During Phase IV (13,360–11,800 cal yr BP) , Lago Airo functioned as a lotic-like ecosystem connected to the Rio Negro. This phase was characterized by high-energy conditions and low diatom sedimentation. The low nutrient levels and high C/N ratio indicate an environment with limited organic matter input and reduced primary production, likely due to the high-energy, flowing water dynamics of the system. Around 13,400 cal yr BP the lake exhibited higher sediment deposition which may be explained by the contemporaneous high precipitation during and just after the last deglaciation. In the upper Rio Negro River at Lagoa da Pata, after 15,500 cal yr BP until 10,000 cal yr BP occurred a substantial increase in lake level attested by increase phytoplanktonic organic matter (Cordeiro et al. 2011 ). During Phase III (11,800–8,800 cal yr BP) , the sand percentage decreased, while higher proportions of silt and clay were observed (Fig. 4). These grain size variations reflect changes in the system's hydraulic energy, with reduced sand deposition indicating a decline in fluvial influence. This phase also shows a progressive increase in TOC and a high C/N ratio, suggesting enhanced transport of detrital organic matter from the surrounding basins. While the environment remained lotic, it exhibited signs of increased organic input and possibly higher productivity compared to Phase IV. In Acarabixi Lake (Rodríguez-Zorro et al. 2017 ) during the early Holocene, a highly dynamic floodplain lake in which forest vegetation occurred with direct influence by a channel of the Rio Negro from 10,840 to 8240 cal yr BP. According to Rodríguez-Zorro et al. ( 2017 ), Rio Negro C/N ratios are particularly high considering the record obtained in Acarabixi, due to the influence of detritic organic matter and humic acids, highly deficient in nitrogen. The high C/N ratios found on Lago Airo reveal also a great influence of Rio Negro over the lake at that specific time. Similarly, high C/N ratio values were found on Acarabixi lake records, situated nearby (Rodríguez-Zorro et al. 2017 ). In Roraima, North hemisphere Equatorial zone with Cerrado vegetation, Lake Caracaranã was formed around 11,500 cal yr BP (Simões Filho 2000 , Cordeiro et al. 2014 , Beltran et al. 2023 ) with evidence of dry periods around 10,000-7800 cal yr BP (Simões Filho 2000 , Cordeiro et al. 2014 , Beltran et al. 2023 ). This phase was characterized by relatively low diatom density (valves/g) with mainly epiphytic taxa specifically Eunotia spp. ( E. hirudo, E. bilunaris , and E. flowerii ). This genus occurs on acid, oligotrophic/distrophic and freshwater environments (Round et al. 1990 , Spaulding et al. 2021 ), which explains its high occurrence in Lago Airo. The presence of Aulacoseira distans and A. granulata indicates that these species have ecological flexibility. While they are primarily limnophilic and prefer still water environments, their occurrence in flowing water suggests they can adapt to dynamic hydrological conditions (Moro and Fürstenberger, 1997 ) Phase II (8,800–4,100 cal yr BP) is characterized by continued high-energy conditions, with coarse sediments predominating. The high C/N ratios and elevated TOC levels suggest a substantial contribution of detrital organic matter, indicating increased external organic input but limited primary production. This phase corresponds to the mid-Holocene dry period (Mayle and Power, 2008 ), during which many Amazonian sites experienced a decline in lake levels (Cordeiro et al. 1997 , 2008) and a reduction in forest biomass (Bush et al. 2007 , Behling et al. 2001 , Absy et al. 1991 , Fontes et al. 2017 ). In Lago Airo it is noted that at 8,000 cal yr BP there is an abrupt decrease in the density of diatom valves with subsequent variability and a substantial decrease until approximately 3,700 cal yr BP. A study carried out by Bush and Colinvaux ( 1988 ) on lake sediment in Ecuador (Lago Ayauch, Ecuador (500 m) showed horizons of laminated sediment and weathered gyttja (lake sediment rich in organic matter), showing a reduction in the water level in the period 4,200-3,150 years B.P., confirmed by the absence of open water diatoms and the abundance of sponge spicules. The resumption of gyttja deposition around 3,150 years B.P. indicated that the lake level, and presumably rainfall, had begun to increase At phase I (ca 4,100 cal yr BP to present) the Rio Negro disconnects from Lago Airo, as suggested by the predominance of silt and clay, indicating a transition between a predominantly erosive environment (lotic-like condition) to a lower energy environment, producing a lentic ecosystem on Lago Airo. There is also a significant decrease on C/N ratio, which indicates a diminishing of the river influence over the lake. Analysis of Corg/Ntotal atomic ratio, δ 13 C and δ 15 N of organic matter can allow distinguishing between algae and vascular plant contributions to sediment organic matter (Meyers and Ishiwatari 1993 , Meyers 2003 ). The higher δ 15 N rates during phase II additionally with the low values of C/N ratio, suggest the development of lacustrine productivity in agreement with the rising of silt and clay grain size on records. It was also recorded the highest diatom density during this phase, with planktonic species overcoming, such as Aulacoseira distans and A. granulata , whereas Aulacoseira cf. calypsi reappeared on record. Aulacoseira is the most representative genera in Lago Airo diatoms composition. This phase marks a transition from a lotic to a lentic system. The increase in diatom density, shift to finer sediments, and higher TOC content reflect a more stable, low-energy environment with higher primary production. The lower C/N ratio indicates increased autochthonous production, suggesting diminished influence from the Rio Negro and the development of a lacustrine ecosystem. Aulacoseira spp. are a good indicator of turbulent waters, because its heavy frustules require high water movement to remain on the photic zone (Wolin and Stone 2010 ). The dominance of Aulacoseira spp. indicated a lentic system on phase I after lateral displacement of the Rio Negro main channel or decrease of Rio Negro water level forming a shallow lake on Lago Airo region. This phase is considered the wettest in Amazonia principally after 3,000 cal yr BP, when the climate becomes progressively more humid and probably with less intranual hydric variability. This condition favoring the installation of a slower environment of greater productivity and potency of organic matter accumulation. Analogous to Lago Airo area, the Acarabixí lake (00º20' S; 64º29' O) located at a fluvial plain of the Rio Negro (AM) demonstrated lateral displacement of the main channels of the river throughout time (Rodriguez -Zorro et al. 2017). During the Late Holocene, according to Helama et al. ( 2017 ) the ‘Dark Ages Cold Period’ (DACP) is probably the climate anomaly most frequently discussed of the first millennium. Also, the Medieval Climate Anomaly (MCA, ca. 950–1200 AD ) and the Little Ice Age (LIA, ca. 1400–1700 AC ) are important climate events that occurred during the Late Holocene (Mann et al. 2009 ). The MCA is considered the more recent warm interval from the pre-industrial period and had a great influence on US and Europe climate. Nevertheless, the causes of this climate anomaly are uncertain (Mann 2002 ). On the opposite, the most recent and longer episode of glacier advance and temperature drop throughout the Holocene is registered at the Little Ice Age with a global scale effect (Chambers et al. 2014 ). A climate simulation model confirmed the global scale effects of both climate events (Mann et al. 2009 ). Here we discuss these climatic events at the Rio Negro Basin (and Amazon Basin overall) and the possible forcings behind these paleoclimate periods. 5.1.1. The Younger Dryas The Younger Dryas was an abrupt and global climate event that occurred in ca. 12.9–11.7 ka (Cohen et al. 2018 ). Meanwhile, during the same period, there is evidence of the Atlantic Meridional Overturning (AMOC) weakening (MacManus et al. 2004). AMOC reduction would have been responsible for cooling in the Northern Hemisphere and warming in the Southern Hemisphere by moving the ITCZ to South (Carlson 2010 ). In the Southern Hemisphere, the temperature in Antarctica showed warming at the Early Holocene period (between 11,000- and 9,000-years BP) (Masson et al. 2000 ). Cariaco basin, on Venezuela, showed a shift from a warm and wet to a cold and dry condition at YD (Lea et al. 2003 ) and authors believe that there was a southward shift of the ITCZ during the period. Concurrent, Lake Titicaca indicated maximum water level (Baker et al. 2001 ). The results obtained in Lago Airo showed evidence that during YD climatic conditions in the region were more humid. The lowest average TOC (1.34%) as recorded. As seen previously, the low TOC concentration is related to high hydrodynamic energy, which may represent a strong fluvial influence at the lake. 5.1.2. Early (11,700- 8,200 Years BP) to Mid Holocene (8,200–4,200 Years BP) The period of the early-mid-Holocene represented an intermediary climate condition in records all over the Amazon region (De Freitas et al. 2001 , Moreira et al. 2013a , Cordeiro et al. 2008, 2014 ). Chemical analysis of Lake Valencia, Venezuela (Lewis and Webezahn 1981 ) showed that after the last glacial maximum the lake was ephemeral until 10,500 years B.P. From then on there was a decrease in salinity associated with a wet period in 8,000 years B.P. From 8,000 years B.P. to 7,500 years B.P. salinity increased suggesting a period of intensified dry conditions. Clayey clastic material was deposited between 7,400 and 6,000 years B.P. with low levels of sedimentary chlorophyll suggesting a return to dry conditions. Pollen analysis in the same lake showed changes in vegetation after the last glacial period, with forest-semideciduous vegetation appearing between 9,800 and 8,300 years B.P. (Leyden 1985 ). Between 8,300 and 5,200 years B.P. there was an expansion in savannah vegetation. In Lake Acarabixi, middle Rio Negro basin, a gap of sedimentation was observed between 8,200–1,500 cal yrs B.P. after a substantial decrease in carbon rates that coincides with the dry phase of the Holocene in Carajás and low water events in Roraima and Humaitá (Cordeiro et al. 2008). Between 8,700 and 5,800 cal yr BP, it was observed dry events in Ecuador, western Amazon (Weng et al. 2002 ). Concurrently, the highest frequency of forest fires in the Amazon region were recorded for ca. 8,000–4,000 cal yr BP (Cordeiro et al. 2014 ). A middle Holocene dry event was also documented in the Peruvian Amazon that appears to have lasted from ca. 7,200 cal yr BP until ca. 3,300 cal yr BP (Bush et al. 2007 ). Climate simulations detected an intensification of seasonal winds at this period, which may have caused the diminishing of wind convergence upon the Amazonian region and this condition might explain the precipitation rates’ drop in this region during this time (Melo and Marengo 2008 ). On early-mid-Holocene, the Lago Airo area showed low diatoms density, however at some periods presenting peaks of planktonic diatoms. These peaks on ca. 10,930 cal yr BP and 9,100 could represent a formation of a lentic system, intervals in which the Rio Negro disconnected of Lago Airo area, at a period when the Rio Negro connection with Lago Airo Area was strong. The records of the early-mid-Holocene on Lago Airo area represented a mainly lotic phase inferred by coarse grains results. 5.1.3. Late Holocene (4,200 Years BP to present). During the late Holocene, the Amazon region was more humid than early mid-Holocene (Moreira et. al. 2013a ). We interpreted the possible isolation of Lago Airo area from the Rio Negro as an indicative of a wetter condition, where highly dense vegetation might have isolated the connection with the river and formed a lake as is currently observed. This suggestion can be reinforced by the diminished C/N ratio and sand concentration. With lake isolation, the organic matter was less diluted, so the TOC presents high values during this period. A wet condition in the Late Holocene was also registered at several regions at the Amazon rainforest (Fig. 8 ): Mayle et al. ( 2000 ) reported that humid evergreen rainforests on the southern margin of Amazonia expanded due to increased precipitation after 2790 cal yr BP. Similarly, increased lacustrine productivity was observed in the Carajás region (~ 2800–1300 cal yr BP) at 800 m elevation outside the Amazon Basin's floodplain (Cordeiro et al., 2008), indicated by higher chlorophyll derivatives and TOC accumulation rates. Comparable trends were reported in the eastern Colombian Amazon (Behling and Hooghiemstra, 2000 ), and central Amazon (Behling et al., 2001 ). Some authors indicate that this condition was linked with the northward shift of ITCZ position (Behling and Hooghimiestra 2000, Silva Dias et al. 2009 ). In Calado Lake, 80 km from the confluence of the Amazonas River and the Rio Negro, Behling et al. ( 2001 ) found longer periods of high water in 4,070 cal yr BP. In the Llanos Orientales, Behling and Hooghiemstra ( 2000 ) found wetter climatic conditions after ca. 3,500-year BP, when according to them, grassland savanna decreased. In Lago Airo area a true-lake formation was inferred since the last ca. 2000 years due to more frequent isolation events of Lago Airo from the Rio Negro. In Lake Maracá in eastern Amazonia Sedimentation resumed (~ 3600–2700 cal yr BP) with reduced Amazon River inflow. Full hydrological connection with the river was established by 1880 cal yr BP, increasing sediment inputs from the Andes, altering mineral composition, organic matter sources, and soil pH, reflecting rising water levels (Moreira et al., 2013b ). Geochemical analyses of Preto Lake sediments revealed Solimões River hydrological changes during the late Holocene. From 3600–400 cal yr BP, high river inflow maintained lake stage, contributing smectite-rich sediments and phytoplankton-derived organic matter. Over the last 400 cal yr, reduced river inflow and increased C3-plant runoff marked the lake's semi-isolation, likely caused by sediment accretion forming a natural levee (Moreira et al. 2020 ). The ‘Dark Ages Cold Period’ (DACP) is the most frequently discussed climate anomaly of the first millennium, apparently, it occurred around 1,540 cal BP until 1,175 cal BP. (410 AD and 775 AD). This event has been considered as cold, reported by studies around the Northern Hemisphere (Helama et al. 2017 ). The same authors indicate that DACP might have proceeded because of a combination of factors, particularly volcanic and reduced solar activity. Over the DACP beginning, the diatoms density records (valves/g) increased like never seen before on Lago Airo and the P:B diatoms ratio resulted on planktonic diatoms dominancy that indicates open water on a lentic system. These results showed that a true lentic system was in formation under this climatic event, that started with a lotic-like system at the beginning (ca. 4,200–3,600 years BP) of the Late Holocene, being influenced by the Rio Negro floodplain. After 1,000 cal yr BP in an isolate lake from the river influence at Carajás region (Cordeiro et al. 2008a ) as well as in the floodplain areas of the Amazon River (Moreira et al. 2004), the last 1000 years cal BP were the wettest period on record. This pattern is already identified at low latitudes in the Northern Hemisphere in Roraima Savannas (Beltran et al. 2023 ) that also experienced wetter conditions and established the current lake level and climate after 1000 years. In the Amazonas River lowlands, Lake Grande do Curuai and Maracá Lake showed the higher rate of sedimentation recorded since 2,700 cal yr BP due to the discharge of the Amazon River, caused by changes in ITCZ frequency southwards (Moreira-Turcq et al. 2014 ). According to the planktonic diatoms’ dominancy interpretation, at DACP Lago Airo water-level started to increase to nowadays’ levels, which corroborates with the argument of the shift of ITCZ frequency southwards. It seems that during the Little Ice Age (ca. 300 years BP) a strong SASM probably reinforced the South Atlantic Subtropical Anticyclone, limiting the southward shift of the ITCZ and bringing drier conditions to northeast Brazil, a mechanism already suggested for the early and middle Holocene (Cruz et al. 2009 ) and for the late Holocene (Novello et al. 2012 ). P:B diatoms ratios on Lago Airo indicated that LIA had less wet conditions than DACP and MCA (See Fig. 8 ). In Fig. 8 we presented a plot of humid and dry periods from the end of Pleistocene to Holocene on South America latitudinal geographic coordinates 4ºN to 8ºS according to several authors. 6. Conclusion The integration of diatom assemblages, sedimentological data, and geochemical indicators reveals a clear trajectory of environmental change in Lago Airo over the past 13,300 years, shedding light on paleohydrological variability in the Amazonian Rio Negro basin. This provides valuable insights into the climatic variability from the end of the Pleistocene to the Holocene in the Amazon basin. The integration of the data highlights the lake's ecological dynamics. Over the past 13,300 years, Lago Airo has undergone significant environmental changes driven by hydrological and sedimentary dynamics. During Phase IV (ca. 13,400–11,800 cal yr BP), the lake exhibited high-energy lotic conditions, dominated by oligotrophic and periphytic species such as Eunotia hirudo and E. bilunaris , with coarse sediments and low diatom density reflecting strong fluvial influence and low nutrient availability. In Phase III (11,800–8,800 cal yr BP), the system transitioned toward more stable conditions, marked by increased clay content, slightly higher TOC values, and continued dominance of periphytic diatoms. These changes indicate reduced fluvial influence, moderate detrital input, and a gradual shift toward greater sedimentation stability and slightly higher productivity. Phase II (8,800–4,100 cal yr BP) saw the emergence of planktonic species like Aulacoseira spp., signaling a transition to more lentic conditions with diminished Rio Negro influence. This phase aligns with mid-Holocene dry conditions, lower lake levels, and increased organic input with terrestrial source. By Phase I, 4,100 cal yr BP to present, the lake had fully transitioned to a lentic environment, characterized by finer sediments, higher diatom density, and dominance of planktonic species. This phase reflects eutrophic conditions, increased productivity, and the wettest climatic period in Amazonia over the past millennia, promoting ecosystem stabilization and organic matter accumulation. This hydrological variability indicates a dry early-mid-Holocene climate with extreme events at the Medium Rio negro Basin compared to a wetter and more stable late Holocene, which would be attributed to shifts in the position of the ITCZ and South American Monsoon System. Around 4,000 years ago, under a wetter climate and more regular rainfall, Lago Airo transitioned to a lentic environment as a result of this new hydroclimatic regime, establishing more eutrophic conditions characterized by finer sediments, higher diatom density, and increased productivity. This contrasts with the Middle Holocene, when the Rio Negro likely transported coarser sediments during extreme climatic events. Our study showed lower levels of water along with less influence of Rio Negro water’s on Lago Airo from 13,400 years to 4,100 years BP (Phases IV, III, and II, which could be inferred by fewer diatom valves/g, increasing percentages of silt, high C/N ratio and low values of δ15N. Whereas from 4,000 years BP to recently (Phase 1, the Rio Negro disconnected from Lago Airo and an increase in water level occurred, characterized by higher valves/g, planktonic taxa dominance, and δ 15 N values increase. Declarations Author Contribution LRP, JCCV, DFG, DF, and RCC: wrote the main manuscript textPM-T, BT, LSM, and RCC: conceptualization JDADC, GSM, LRP, JCCV, DFG, DF, AB: Data curation , Formal analysis Acknowledgement We are grateful for the support of the Bilateral Scientific Cooperation Project IRD-França/CNPq, Laboratório Mixto Internacional PALEOTRACES and the "INSU-EC2CO Dynamique du carbone et les changements climatiques dans le Bassin Amazonien. The authors acknowledge research support from the “Conselho Nacional de Pesquisa” (CNPq) process: 3479873/2013-5, 308222/2015-6 and “Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro” (FAPERJ) process: E-26/111.625/2011. Also, for the financial aid of the Project "RED0026/2014 (Fapesb) - Reconstrução Paleoambiental da Baía de Camamu: Implicações para a Gestão Ecossistema" and for the support of the EcoPaleo lab (Biology Institute, UFBA, BR). We are thankful for the assistance of Dr. Abdelfettah Sifeddine (IRD, France), and Fundação Oswaldo Cruz - Fiocruz (Salvador/BA, BR). This study was financed in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - Brasil (CAPES) - Finance Code 001. We also acknowledge the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq, Brazil) for a Research Productivity Fellowship (PQ) References Absy, ML, Cleff, A, Fournier, M, Martin, L, Servant, M, Sifeddine, A, Ferreira DA Silva,M, Soubies, F, Suguio, K, Turcq, B, Van Der Hammen, TH, (1991) Mise en évidence dequatre phases d'ouverture de la forêt dense dans le sud-est de l'Amazonie au coursdes 60000 dernières années. P Première comparaison avec d'autres régions tropicales.C.R. Acad. 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03:02:24","extension":"xml","order_by":24,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":189267,"visible":true,"origin":"","legend":"","description":"","filename":"28a231555add49ca95a8e17c33cf0c1f1structuring.xml","url":"https://assets-eu.researchsquare.com/files/rs-7687264/v1/e454fea1d64ee7c42cfb94fc.xml"},{"id":93641979,"identity":"be097de6-7630-428e-ae35-6835c1c15161","added_by":"auto","created_at":"2025-10-16 03:02:24","extension":"html","order_by":25,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":198792,"visible":true,"origin":"","legend":"","description":"","filename":"earlyproof.html","url":"https://assets-eu.researchsquare.com/files/rs-7687264/v1/0f1bb8864a3ab65336253d05.html"},{"id":93642494,"identity":"ffc2d206-481d-42e5-acad-c33e337c74aa","added_by":"auto","created_at":"2025-10-16 03:10:23","extension":"png","order_by":1,"title":"Figure 1","display":"","copyAsset":false,"role":"figure","size":5713,"visible":true,"origin":"","legend":"\u003cp\u003eA. Satellite view showing the location of Lago Airo in relation to the Rio Negro, with the lake situated within the floodplain area. The white rectangle highlights the position of the lake. B. Close-up satellite image of Lago Airo and the surrounding floodplain landscape with campinarana vegetation in brown and ombrofilous vegetation ion green. The lake is shown in proximity to the Rio Negro, emphasizing its hydrological connection. C. Map of Brazil highlighting the state of Amazonas (shaded in gray), where Lago Airo is located. D. Hydrological map of the Rio Negro basin near Lago Airo, depicting the floodplain system (blue) and vegetative cover (green). The star symbol marks the location of Lago Airo within the basin.\u003c/p\u003e","description":"","filename":"placeholderimage.png","url":"https://assets-eu.researchsquare.com/files/rs-7687264/v1/45a0ac0dc54da00b028be1ab.png"},{"id":93642751,"identity":"79284cfb-6d5e-4e3f-8700-618b3547156a","added_by":"auto","created_at":"2025-10-16 03:18:23","extension":"png","order_by":2,"title":"Figure 2","display":"","copyAsset":false,"role":"figure","size":71843,"visible":true,"origin":"","legend":"\u003cp\u003eCluster analysis (paired group, Euclidean distance) using the distribution of diatom flora. The green arrows indicate the sections where lithological contacts occur, as described in table 1. Some lithological units are associated with changes in the diamond assemblage, while others do not show significant ecological changes that could not incorporate changes in sedimentary facies.\u003c/p\u003e","description":"","filename":"2.png","url":"https://assets-eu.researchsquare.com/files/rs-7687264/v1/c8df638fb9bfaa09676ccca2.png"},{"id":93641948,"identity":"af302f86-8bb9-47e1-8f6f-4424c64ce4f6","added_by":"auto","created_at":"2025-10-16 03:02:23","extension":"png","order_by":3,"title":"Figure 3","display":"","copyAsset":false,"role":"figure","size":263129,"visible":true,"origin":"","legend":"\u003cp\u003eLithological profile, calibrated radiocarbon ages, sedimentation \u0026nbsp;rate, and stratigraphic zones from Lago Airo. The lithology column shows sediment textures and colors with Munsell color notations. Radiocarbon ages (cal yr BP) are plotted against depth (cm) alongside the calibrated age model (solid red line) with confidence intervals (dashed gray lines). Sedimentation \u0026nbsp;rates (cm/yr) are displayed on the right. Four stratigraphic zones (I–IV) are indicated based on changes in sedimentological characteristics and age-depth relationships.\u003c/p\u003e","description":"","filename":"3.png","url":"https://assets-eu.researchsquare.com/files/rs-7687264/v1/80121e81225f8e4e978e35b6.png"},{"id":93642496,"identity":"e6f4946c-d466-41d0-a65a-9874777fd8d4","added_by":"auto","created_at":"2025-10-16 03:10:23","extension":"png","order_by":4,"title":"Figure 4","display":"","copyAsset":false,"role":"figure","size":153380,"visible":true,"origin":"","legend":"\u003cp\u003eDescription of the Airo 12/01 core, grain size distribution, Total Organic Carbon (TOC), C/N, δ\u003csup\u003e13\u003c/sup\u003eC and δ\u003csup\u003e15\u003c/sup\u003eN. Phases are represented with horizontal white and gray markings.\u003c/p\u003e","description":"","filename":"4.png","url":"https://assets-eu.researchsquare.com/files/rs-7687264/v1/961677c65e5297a36615d6c8.png"},{"id":93641952,"identity":"e8547b52-c427-4e5a-b1ed-4a5e560a2f04","added_by":"auto","created_at":"2025-10-16 03:02:23","extension":"png","order_by":5,"title":"Figure 5","display":"","copyAsset":false,"role":"figure","size":185874,"visible":true,"origin":"","legend":"\u003cp\u003ePlot of relative abundances (%) of main diatom species along the Airo 12/01 core and phases limits.\u003c/p\u003e","description":"","filename":"5.png","url":"https://assets-eu.researchsquare.com/files/rs-7687264/v1/8d2a4034f90a452f7f29111d.png"},{"id":93642752,"identity":"9cd19c31-da7a-4203-ab41-38a0913d5aad","added_by":"auto","created_at":"2025-10-16 03:18:23","extension":"png","order_by":6,"title":"Figure 6","display":"","copyAsset":false,"role":"figure","size":148095,"visible":true,"origin":"","legend":"\u003cp\u003ePlot of diatom density (valves/g), Planktonic (“P”) and Benthic (“B”) diatoms and P:B anomaly from the Airo 12/01 core (\u0026lt;5.0) evidencing the following climate phenomena YD, DACP, MCA and LIA. Dates of the Pleistocene and Holocene are according the International Chronostratigraphy (Cohen et al., 2018).\u003c/p\u003e","description":"","filename":"6.png","url":"https://assets-eu.researchsquare.com/files/rs-7687264/v1/5761db37135ad9191ce588e1.png"},{"id":93642497,"identity":"488515c9-0b51-4867-a538-0181babbe893","added_by":"auto","created_at":"2025-10-16 03:10:23","extension":"png","order_by":7,"title":"Figure 7","display":"","copyAsset":false,"role":"figure","size":121521,"visible":true,"origin":"","legend":"\u003cp\u003ePlot of diatom density (valves/g), and factor 1 from component principal analyses from the Airo 12/01 core. Factor 1 is controlled in order of importance by: \u003cem\u003eEunotia sp3, Aulacoseira\u003c/em\u003e \u003cem\u003e\u0026nbsp;ambigua, Aulocoseira distans, Aulocoseira granulate\u003c/em\u003e.\u003c/p\u003e","description":"","filename":"7.png","url":"https://assets-eu.researchsquare.com/files/rs-7687264/v1/4e4bd9b9b525a8662d0a940d.png"},{"id":93682553,"identity":"562bd20e-6a20-4ff7-93c3-ade49e9a92d9","added_by":"auto","created_at":"2025-10-16 12:34:26","extension":"pdf","order_by":0,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":2170065,"visible":true,"origin":"","legend":"","description":"","filename":"manuscript.pdf","url":"https://assets-eu.researchsquare.com/files/rs-7687264/v1/593757a4-a646-4135-a223-bccedf014af7.pdf"},{"id":93643570,"identity":"a2e82b76-a5c8-4b20-8f4b-cd1e373b1bb0","added_by":"auto","created_at":"2025-10-16 03:34:23","extension":"docx","order_by":0,"title":"","display":"","copyAsset":false,"role":"supplement","size":377177,"visible":true,"origin":"","legend":"","description":"","filename":"SupplementaryMaterialLagoAiro.docx","url":"https://assets-eu.researchsquare.com/files/rs-7687264/v1/f5bb407e2a83c84ec3e4e5bf.docx"}],"financialInterests":"No competing interests reported.","formattedTitle":"Paleoclimate variations in Western Amazon based on Lago Airo (Brazil) diatoms from the last 13,300 years cal BP","fulltext":[{"header":"1. Introduction","content":"\u003cp\u003eThe Amazon region has substantial participation on heat distribution and humidity release from the Tropics, influencing in climate and precipitation rates from global to local scales (Werth and Avissar \u003cspan citationid=\"CR77\" class=\"CitationRef\"\u003e2002\u003c/span\u003e). The main climate influence of the Amazon is the presence of moisture from the Amazon Forest from eastern Brazil (e.g. South American Summer Monsoon - SASM) and the displacement of the Inter-Tropical Convergence Zone - ITCZ (Cruz et al. \u003cspan citationid=\"CR26\" class=\"CitationRef\"\u003e2009\u003c/span\u003e). In respect of distinct climatic variabilities at different time scales, wet and dry conditions at the Amazon basin are related throughout the Holocene, and these periods are controversial (Silva et al. \u003cspan citationid=\"CR71\" class=\"CitationRef\"\u003e2018\u003c/span\u003e).\u003c/p\u003e\u003cp\u003eThe ITCZ is a band of clouds induced by low-level convergence of Tradewinds over equatorial oceans, that promotes a minimum in atmospheric pressure, a low mixed-layer depth, a maximum of Sea Surface Temperature (SST), and deep convective rainfall (Garreaud et al. \u003cspan citationid=\"CR33\" class=\"CitationRef\"\u003e2008\u003c/span\u003e). The Amazon River basin and Amazon Forest contribute to climate dynamics on Tropical South America being affected by complex dynamics teleconnections (Potter et al. \u003cspan citationid=\"CR63\" class=\"CitationRef\"\u003e2004\u003c/span\u003e), and astronomical forcing (e.g. insolation changes explained by Milankovitch cycles) (Silva Dias et al. \u003cspan citationid=\"CR70\" class=\"CitationRef\"\u003e2009\u003c/span\u003e).\u003c/p\u003e\u003cp\u003eThe Rio Negro (Amazon River basin) is the largest black water river in the world, with a basin area of around 700,000 km2 passing through Colombia, Venezuela, and Brazil (Latrubesse and Franzinelli, \u003cspan citationid=\"CR39\" class=\"CitationRef\"\u003e2005\u003c/span\u003e). According to these authors its formation is dated back to the Late Pleistocene and Holocene, associated with activity during the last glacial in the Amazon Basin. However, paleoecological studies in remote areas like the upper part of Rio Negro are scarce (Rodr\u0026iacute;guez-Zorro et al. \u003cspan citationid=\"CR66\" class=\"CitationRef\"\u003e2017\u003c/span\u003e). The analysis of three sediment records in three lakes of the Morro dos Seis Lagos stream in the upper Rio Negro showed no evidence of changes in vegetation physiognomy during the late Pleistocene and Holocene (Colinvaux et al. \u003cspan citationid=\"CR20\" class=\"CitationRef\"\u003e1996\u003c/span\u003e, Bush et al. \u003cspan citationid=\"CR16\" class=\"CitationRef\"\u003e2004\u003c/span\u003e), rather than significant evidence of changes in lake levels (Bush et al. \u003cspan citationid=\"CR16\" class=\"CitationRef\"\u003e2004\u003c/span\u003e, Cordeiro et al. \u003cspan citationid=\"CR25\" class=\"CitationRef\"\u003e2011\u003c/span\u003e). Changes in atmospheric patterns between the end of glaciation and the beginning of the warmer conditions of the Holocene were observed through changes in mercury fluxes in the Lagoa da Pata (Barbosa et al. \u003cspan citationid=\"CR5\" class=\"CitationRef\"\u003e2004\u003c/span\u003e).\u003c/p\u003e\u003cp\u003eThe aim of this study was to infer paleolimnological dynamics and paleoclimate conditions at western Amazon, based on Lago Airo (Rio Negro basin) sediment core and major studies in the area. This research was based on a multiproxy approach that included geochemistry, multiple sediment variables analyses and diatom assemblage. Our results assist in understanding paleolimnological and climate variations insights throughout the Holocene and Pleistocene/Holocene transition of the Amazon basin, from registers of a lake located at extreme northwestern Brazil.\u003c/p\u003e"},{"header":"2. Site Description","content":"\u003cp\u003eLago Airo is a floodplain lake in Rio Negro basin (0\u0026deg;19\u0026rsquo;37.225\u0026rdquo; S, 66\u0026deg;8\u0026rsquo;33.266\u0026rdquo; W), Municipality of S\u0026atilde;o Gabriel da Cachoeira city, Amazonas State (Fig.\u0026nbsp;\u003cspan refid=\"Fig2\" class=\"InternalRef\"\u003e1\u003c/span\u003e). Rio Negro is the largest tributary of Amazon River, considered the fifth largest river in the world (Franzinelli and Igreja \u003cspan citationid=\"CR31\" class=\"CitationRef\"\u003e2002\u003c/span\u003e). The Negro River the most important black water river and the second largest tributary of the Amazon River drains an area of 696,810 km\u003csup\u003e2\u003c/sup\u003e, with a discharge of about 28,400 m\u003csup\u003e\u0026minus;\u0026thinsp;3\u003c/sup\u003es\u003csup\u003e\u0026minus;\u0026thinsp;1\u003c/sup\u003e and provides an average of 8 Mt. yr\u003csup\u003e\u0026minus;\u0026thinsp;1\u003c/sup\u003e of suspended sediment flux.\u003c/p\u003e\u003cp\u003e\u003c/p\u003e\u003cp\u003eLago Airo has approximately three meters of depth in the collecting date, maximum width of 300 m, pH around 3.5, and nowadays has no connection with Rio Negro. Located on equatorial region, in the Amazon Forest biome, with a hot and humid climate. The annual rainfall average on S\u0026atilde;o Gabriel da Cachoeira city area is 2,853 mm (Ribeiro et al. \u003cspan citationid=\"CR65\" class=\"CitationRef\"\u003e1996\u003c/span\u003e).\u003c/p\u003e\u003cp\u003eThe vegetation around the lake is \u003cem\u003eCampinarana\u003c/em\u003e, which is composed by 12 m \u003cem\u003earboreum strata\u003c/em\u003e distributed continuously with scarce open fields. The sunlight reaches the soil. It is a vegetation which occurs on hydromorphic Podzolic soils, with hydromorphic quartz sands on the alluvial plain of the Negro River. The \u003cem\u003ecampinaranas\u003c/em\u003e occur on flat and flooded areas around the lake, and present quite varied physiognomy as observed in the region with campestral and forest formations. Vegetation is characterized by soil rich in organic matter, and slow decomposition due to low pH values and large aluminum concentration (Martins and Matthes1978).\u003c/p\u003e\u003cp\u003e\u003c/p\u003e"},{"header":"3. Material and Methods","content":"\u003cdiv id=\"Sec4\" class=\"Section2\"\u003e\u003ch2\u003e3.1. Core Sampling and Radiocarbon chronology.\u003c/h2\u003e\u003cp\u003eSediments from Lago Airo were collected in 2015 using a vibro-coring system equipped with aluminum tubes measuring 7.6 cm in diameter. The sampling platform consisted of two rubber boats, which facilitated the retrieval of the AIRO 12/01 core, measuring 146 cm in length (0\u0026deg;19'37.225\" S, 66\u0026deg;8'33.266\" W). The description of the sedimentary profile was carried out as soon as the core was opened, and its lithology was described with the help of a Munsell Colour Chart. The sediment core was sliced into 1.0 cm sections which were stored in plastic bags. Seven calibrated \u003csup\u003e14\u003c/sup\u003eC dates of bulk organic matter based the age-depth model. Measurements of \u003csup\u003e14\u003c/sup\u003eC activity were made by Acceleratory Mass Spectrometry (AMS) at the Radiocarbon Dating Laboratory Beta Analytic, located in Miami, Florida, Laboratoire de Mesure du Carbone 14 (Table\u0026nbsp;\u003cspan refid=\"Tab1\" class=\"InternalRef\"\u003e1\u003c/span\u003e). The program Calib 7.1 (Stuiver et. al. \u003cspan citationid=\"CR75\" class=\"CitationRef\"\u003e2018\u003c/span\u003e) was used for calibration due to the natural variations of 14C (available at: \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003ehttp://calib.org/calib/\u003c/span\u003e\u003cspan address=\"http://calib.org/calib/\" targettype=\"URL\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e), and dates were converted to cal BP. To model the age-depth relationship, the R package \u0026ldquo;Bacon\u0026rdquo; (Blaauw and Christen, 2011) was used, which adopts a Bayesian approach to establish a robust chronological sequence. This model integrates available radiometric dates and, through the IntCal 13 calibration curve, converts these dates into calibrated ages (cal yr BP), providing an accurate temporal reconstruction of the observed sedimentary variations along the profile.\u003c/p\u003e\u003c/div\u003e\u003cdiv id=\"Sec5\" class=\"Section2\"\u003e\u003ch2\u003e3.2. Grain-size Distributions\u003c/h2\u003e\u003cp\u003eA laser particle analyzer (CILAS Particle Analyzer\u0026reg; 1064) was used to measure the grain-size distribution of the mineral fraction (particles between 0.04 and 500 \u0026micro;m) after the organic matter and carbonate destruction with H\u003csub\u003e2\u003c/sub\u003eO\u003csub\u003e2\u003c/sub\u003e and HCl, respectively, and it is dispersing in a Na\u003csub\u003e4\u003c/sub\u003eP\u003csub\u003e2\u003c/sub\u003eO\u003csub\u003e7\u003c/sub\u003e solution and sonifcation. The program GRADISTAT VERSION 4.0, was used to calculate the granulometric fraction (Blott \u003cspan citationid=\"CR13\" class=\"CitationRef\"\u003e2000\u003c/span\u003e).\u003c/p\u003e\u003c/div\u003e\u003cdiv id=\"Sec6\" class=\"Section2\"\u003e\u003ch2\u003e3.3. Bulk and isotopic organic geochemistry\u003c/h2\u003e\u003cp\u003eThe organic geochemical measurements made included Total Organic Carbon (TOC), Total Nitrogen (TN), δ\u003csup\u003e13\u003c/sup\u003eC (\u0026permil;) and δ\u003csup\u003e15\u003c/sup\u003eN (\u0026permil;). Samples were analyzed for TOC and stable isotopic (δ\u003csup\u003e13\u003c/sup\u003eC and δ\u003csup\u003e15\u003c/sup\u003eN) content to CHN analyzer (PDZ Europa ANCA-GSL) connected to an Isotope Ratio Mass Spectrometer (IRMS) PDZ Europa 20\u0026ndash;20 (Sercon Ltd., Cheshire, UK) at UC Davis Stable Isotope Facility (SIF), Department of Plant Sciences, CA, USA. Samples were treated with 0.5 N HCl to remove carbonate prior to isotopic measurements. It was analyzed lithology, granulometry, total organic carbon (TOC), C:N ratio, δ\u003csup\u003e15\u003c/sup\u003eN and δ\u003csup\u003e13\u003c/sup\u003eC.\u003c/p\u003e\u003cp\u003eThe analysis of chlorophyll derivatives in sediment samples was performed according to the method of Sanger and Gorham (\u003cspan citationid=\"CR68\" class=\"CitationRef\"\u003e1972\u003c/span\u003e), adapted by Cordeiro et al. (\u003cspan citationid=\"CR21\" class=\"CitationRef\"\u003e1997\u003c/span\u003e), (Chlorophyll derivatives were extracted with 90% acetone and measured at 667 nm in Perkin Elmer spectrometer). The results were expressed in arbitrary units as absorbance per gram organic matter, where one unit (SPDU) is equal to an absorbance of 1.0 in a 10 cm cell, when dissolved in 100 ml of solvent.\u003c/p\u003e\u003c/div\u003e\u003cdiv id=\"Sec7\" class=\"Section2\"\u003e\u003ch2\u003e3.4. Diatom assemblages\u003c/h2\u003e\u003cdiv id=\"Sec8\" class=\"Section3\"\u003e\u003ch2\u003e3.4.1. Samples oxidation and slide preparation\u003c/h2\u003e\u003cp\u003eSediment samples oxidation followed the standard method of Battarbee et al. (\u003cspan citationid=\"CR7\" class=\"CitationRef\"\u003e2001\u003c/span\u003e). About 1 g of humid sediment and 30 ml of H\u003csub\u003e2\u003c/sub\u003eO\u003csub\u003e2\u003c/sub\u003e at 37% were add to a beaker. This solution remained for 24h in hot plate (50\u0026deg;C\u0026thinsp;\u0026plusmn;\u0026thinsp;5) until all organic matter was removed. Samples were washed in distilled water until complete dilution of H\u003csub\u003e2\u003c/sub\u003eO\u003csub\u003e2\u003c/sub\u003e. Slides were prepared by dropping 1 ml of diatom suspension on a coverslip. The diatoms were allowed to settle and water to evaporate in a hot plate (50\u0026deg;C\u0026thinsp;\u0026plusmn;\u0026thinsp;5). When dry, slides were mounted using a resin Naphrax\u0026reg; (index\u0026thinsp;=\u0026thinsp;1.74).\u003c/p\u003e\u003c/div\u003e\u003cdiv id=\"Sec9\" class=\"Section3\"\u003e\u003ch2\u003e3.4.2. Identification and Counting\u003c/h2\u003e\u003cp\u003eDiatoms were counted (500 valves per sample) in each 1 (one) cm depth intervals from 0 to 34 cm (except 5\u0026ndash;6 cm and 29\u0026ndash;30 cm due to lack of samples). After that, analyses were carried out each 2 cm intervals, totalizing 86 samples of sediment from Lago Airo. From 34 cm to 145 cm it was set a pattern of 4 transects per slide due to very few amount of diatoms in slides.\u003c/p\u003e\u003cp\u003eDiatoms in each sample were identified to the lowest possible taxonomic level along random transects using an Olympus CX31 light microscope at 1000x magnification. Valves were counted if more than one-third of their original structure was intact, including cases where the valve center was present or when specific morphological features characteristic of the species were observed, to minimize overestimation (Battarbee et al., \u003cspan citationid=\"CR7\" class=\"CitationRef\"\u003e2001\u003c/span\u003e).\u003c/p\u003e\u003cp\u003eIdentification of species was conducted using mainly the following references keys: Patrick and Reimer (\u003cspan citationid=\"CR62\" class=\"CitationRef\"\u003e1975\u003c/span\u003e), Lange-Bertalot (\u003cspan citationid=\"CR37\" class=\"CitationRef\"\u003e1966\u003c/span\u003e, \u003cspan citationid=\"CR38\" class=\"CitationRef\"\u003e2007\u003c/span\u003e), Metzeltin and Lange-Bertalot (\u003cspan citationid=\"CR51\" class=\"CitationRef\"\u003e1998\u003c/span\u003e), DeNicola (\u003cspan citationid=\"CR28\" class=\"CitationRef\"\u003e2000\u003c/span\u003e) and Ferrari et al. (\u003cspan citationid=\"CR30\" class=\"CitationRef\"\u003e2007\u003c/span\u003e).\u003c/p\u003e\u003c/div\u003e\u003cdiv id=\"Sec10\" class=\"Section3\"\u003e\u003ch2\u003e3.4.3. Diatom Analysis\u003c/h2\u003e\u003cp\u003eThe species\u0026rsquo; relative abundance (%) and species\u0026rsquo; density (valves/g) represented the diatom assemblage composition. Absolute abundance value of each species per slides represented the taxa relative abundances. The cut-off value for the species selected as the most representative within the community was 1% of relative abundance at least in 3 depths of the sediment samples. These species\u0026rsquo; relative abundance was classified as benthic or planktonic according to autecological live characters. The diatom density per layer sampled was calculated from the total of valves counted per slide corrected by samples bulk density. The ratios of planktonic to benthic diatoms were calculated using the formula: P:B ratio\u0026thinsp;=\u0026thinsp;planktonic taxa / benthic taxa. A broken-stick model was used (Bennett \u003cspan citationid=\"CR11\" class=\"CitationRef\"\u003e1996\u003c/span\u003e) to determine the number of diatom zones.\u003c/p\u003e\u003c/div\u003e\u003c/div\u003e"},{"header":"4. Results","content":"\u003cp\u003eThrough cluster analyses that incorporate the total diatom assemblage distribution, four primary phases have been established. Accordingly, the subsequent parameter description will encompass these subdivisions (Fig.\u0026nbsp;\u003cspan refid=\"Fig4\" class=\"InternalRef\"\u003e2\u003c/span\u003e).\u003c/p\u003e\u003cp\u003e\u003c/p\u003e\u003cp\u003e\u003c/p\u003e\u003cdiv id=\"Sec12\" class=\"Section2\"\u003e\u003ch2\u003e4.1. Lithology, Chronology and Bulk Density\u003c/h2\u003e\u003cp\u003eThe Airo 12/01 sediment core has a length of 1.46 meters. According to the lithological description provided in Table\u0026nbsp;3, the presence of three main units and five sub-units can be observed. In lithological unit III, which spans from 146 to 91 cm, three sub-units can be identified. The lithological description indicates the occurrence of sandier sediment within this unit. Sub-units IIIc and IIIa exhibited a brown color pattern (referred to as \"brown\" according to the \"Munsell Soil Color Chart\").\u003c/p\u003e\u003cp\u003eLithological unit II consists of two sub-units (IIa and IIb) and spans the interval from 90 to 30 cm. Like lithological unit III, the presence of sandy sediment was noted in unit II. The color pattern observed during the lithological description ranged from dark reddish-brown in sub-unit IIb to very dark gray in sub-unit IIa.\u003c/p\u003e\u003cp\u003eThe first lithological unit represents the uppermost 29 centimeters of the sediment core. A noticeable change in grain size occurs between this unit and unit II, where finer sandy sediment is observed in unit I. The color pattern observed in this lithological unit was black, as per the Munsell Soil Color Chart.\u003c/p\u003e\u003cp\u003e\u003cdiv class=\"gridtable\"\u003e\u003ctable float=\"Yes\" id=\"Tab1\" border=\"1\"\u003e\u003ccaption language=\"En\"\u003e\u003cdiv class=\"CaptionNumber\"\u003eTable 1\u003c/div\u003e\u003cdiv class=\"CaptionContent\"\u003e\u003cp\u003eGeneral description of the lithological units of Airo 12/01 core.\u003c/p\u003e\u003c/div\u003e\u003c/caption\u003e\u003ccolgroup cols=\"6\"\u003e\u003cdiv align=\"left\" class=\"colspec\" colname=\"c1\" colnum=\"1\"\u003e\u003c/div\u003e\u003cdiv align=\"left\" class=\"colspec\" colname=\"c2\" colnum=\"2\"\u003e\u003c/div\u003e\u003cdiv align=\"left\" class=\"colspec\" colname=\"c3\" colnum=\"3\"\u003e\u003c/div\u003e\u003cdiv align=\"left\" class=\"colspec\" colname=\"c4\" colnum=\"4\"\u003e\u003c/div\u003e\u003cdiv align=\"left\" class=\"colspec\" colname=\"c5\" colnum=\"5\"\u003e\u003c/div\u003e\u003cdiv align=\"left\" class=\"colspec\" colname=\"c6\" colnum=\"6\"\u003e\u003c/div\u003e\u003cthead\u003e\u003ctr\u003e\u003cth align=\"left\" colname=\"c1\"\u003e\u003cp\u003eLithological Units\u003c/p\u003e\u003c/th\u003e\u003cth align=\"left\" colname=\"c2\"\u003e\u003cp\u003eSub-units\u003c/p\u003e\u003c/th\u003e\u003cth align=\"left\" colname=\"c3\"\u003e\u003cp\u003eSection (cm)\u003c/p\u003e\u003c/th\u003e\u003cth align=\"left\" colname=\"c4\"\u003e\u003cp\u003eSedimentological characterisation\u003c/p\u003e\u003c/th\u003e\u003cth align=\"left\" colname=\"c5\"\u003e\u003cp\u003eColour Standard\u003c/p\u003e\u003c/th\u003e\u003cth align=\"left\" colname=\"c6\"\u003e\u003cp\u003eColour Code\u003c/p\u003e\u003c/th\u003e\u003c/tr\u003e\u003c/thead\u003e\u003ctbody\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003eI\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c2\"\u003e\u0026nbsp;\u003c/td\u003e\u003ctd align=\"left\" colname=\"c3\"\u003e\u003cp\u003e29\u0026thinsp;\u0026minus;\u0026thinsp;0\u003c/p\u003e\u003cp\u003e3000-Actual\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c4\"\u003e\u003cp\u003eSilt\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c5\"\u003e\u003cp\u003eBlack\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c6\"\u003e\u003cp\u003e5YR 2.5/1\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\" morerows=\"1\" rowspan=\"2\"\u003e\u003cp\u003eII\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c2\"\u003e\u003cp\u003eIIa\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c3\"\u003e\u003cp\u003e52\u0026thinsp;\u0026minus;\u0026thinsp;29\u003c/p\u003e\u003cp\u003e6910\u0026thinsp;\u0026minus;\u0026thinsp;3000\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c4\"\u003e\u003cp\u003eSand with plant fragments\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c5\"\u003e\u003cp\u003eVery Dark Grey\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c6\"\u003e\u003cp\u003e5YR 3.1\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c2\"\u003e\u003cp\u003eIIb\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c3\"\u003e\u003cp\u003e90\u0026thinsp;\u0026minus;\u0026thinsp;52\u003c/p\u003e\u003cp\u003e11630\u0026thinsp;\u0026minus;\u0026thinsp;6910\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c4\"\u003e\u003cp\u003eSand\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c5\"\u003e\u003cp\u003eDark Reddish Brown\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c6\"\u003e\u003cp\u003e5YR 3.2\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\" morerows=\"2\" rowspan=\"3\"\u003e\u003cp\u003eIII\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c2\"\u003e\u003cp\u003eIIIa\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c3\"\u003e\u003cp\u003e101\u0026thinsp;\u0026minus;\u0026thinsp;90\u003c/p\u003e\u003cp\u003e12440\u0026thinsp;\u0026minus;\u0026thinsp;11630\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c4\"\u003e\u003cp\u003eSand\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c5\"\u003e\u003cp\u003eBrown\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c6\"\u003e\u003cp\u003e10YR 4.2\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c2\"\u003e\u003cp\u003eIIIb\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c3\"\u003e\u003cp\u003e112\u0026thinsp;\u0026minus;\u0026thinsp;101\u003c/p\u003e\u003cp\u003e12970\u0026thinsp;\u0026minus;\u0026thinsp;12440\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c4\"\u003e\u003cp\u003eSand with plant fragments\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c5\"\u003e\u003cp\u003eDark Grayish Brown\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c6\"\u003e\u003cp\u003e10YR 3.2\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c2\"\u003e\u003cp\u003eIIIc\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c3\"\u003e\u003cp\u003e146\u0026thinsp;\u0026minus;\u0026thinsp;112\u003c/p\u003e\u003cp\u003e13360\u0026thinsp;\u0026minus;\u0026thinsp;12970\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c4\"\u003e\u003cp\u003eSand\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c5\"\u003e\u003cp\u003eBrown\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c6\"\u003e\u003cp\u003e10YR 4.2\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003c/tbody\u003e\u003c/colgroup\u003e\u003c/table\u003e\u003c/div\u003e\u003c/p\u003e\u003cp\u003eThree radiocarbon dates on total organic matter and four datations from leaf samples were obtained by Accelerator Mass Spectrometry (AMS) in the Airo 12/01 core.\u003c/p\u003e\u003cp\u003e\u003cdiv class=\"gridtable\"\u003e\u003ctable float=\"Yes\" id=\"Tab2\" border=\"1\"\u003e\u003ccaption language=\"En\"\u003e\u003cdiv class=\"CaptionNumber\"\u003eTable 2\u003c/div\u003e\u003cdiv class=\"CaptionContent\"\u003e\u003cp\u003eDescription of radiocarbon ages on AIRO 12/01 core by AMS Method\u003c/p\u003e\u003c/div\u003e\u003c/caption\u003e\u003ccolgroup cols=\"6\"\u003e\u003cdiv align=\"left\" class=\"colspec\" colname=\"c1\" colnum=\"1\"\u003e\u003c/div\u003e\u003cdiv align=\"left\" class=\"colspec\" colname=\"c2\" colnum=\"2\"\u003e\u003c/div\u003e\u003cdiv align=\"left\" class=\"colspec\" colname=\"c3\" colnum=\"3\"\u003e\u003c/div\u003e\u003cdiv align=\"char\" char=\"\u0026plusmn;\" class=\"colspec\" colname=\"c4\" colnum=\"4\"\u003e\u003c/div\u003e\u003cdiv align=\"char\" char=\".\" class=\"colspec\" colname=\"c5\" colnum=\"5\"\u003e\u003c/div\u003e\u003cdiv align=\"left\" class=\"colspec\" colname=\"c6\" colnum=\"6\"\u003e\u003c/div\u003e\u003cthead\u003e\u003ctr\u003e\u003cth align=\"left\" colname=\"c1\"\u003e\u003cp\u003eDepth\u003c/p\u003e\u003cp\u003einterval (cm)\u003c/p\u003e\u003c/th\u003e\u003cth align=\"left\" colname=\"c2\"\u003e\u003cp\u003eDatation code\u003c/p\u003e\u003c/th\u003e\u003cth align=\"left\" colname=\"c3\"\u003e\u003cp\u003eMeasured sample\u003c/p\u003e\u003c/th\u003e\u003cth align=\"left\" colname=\"c4\"\u003e\u003cp\u003eConventional \u003csup\u003e14\u003c/sup\u003eC age\u003c/p\u003e\u003cp\u003e(years BP)\u003c/p\u003e\u003c/th\u003e\u003cth align=\"left\" colname=\"c5\"\u003e\u003cp\u003e2-sigma intercept age\u003c/p\u003e\u003cp\u003e(cal yr BP)\u003c/p\u003e\u003c/th\u003e\u003cth align=\"left\" colname=\"c6\"\u003e\u003cp\u003eAge range 2-sigma\u003c/p\u003e\u003c/th\u003e\u003c/tr\u003e\u003c/thead\u003e\u003ctbody\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003e15 cm\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c2\"\u003e\u003cp\u003eBeta 388870\u0026sup1;\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c3\"\u003e\u003cp\u003eBulk organic matter on Sediment\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\"\u0026plusmn;\" colname=\"c4\"\u003e\u003cp\u003e1170\u0026thinsp;\u0026plusmn;\u0026thinsp;30\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e\u003cp\u003e1065\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c6\"\u003e\u003cp\u003e986\u0026ndash;1179\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003e28 cm\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c2\"\u003e\u003cp\u003eSacA 50900\u0026sup2;\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c3\"\u003e\u003cp\u003eBulk organic matter on sediment\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\"\u0026plusmn;\" colname=\"c4\"\u003e\u003cp\u003e1575\u0026thinsp;\u0026plusmn;\u0026thinsp;30\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e\u003cp\u003e1410\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c6\"\u003e\u003cp\u003e1399\u0026ndash;1537\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003e50 cm\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c2\"\u003e\u003cp\u003eBeta 388871\u0026sup1;\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c3\"\u003e\u003cp\u003ePlant material\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\"\u0026plusmn;\" colname=\"c4\"\u003e\u003cp\u003e4930\u0026thinsp;\u0026plusmn;\u0026thinsp;30\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e\u003cp\u003e5650\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c6\"\u003e\u003cp\u003e5600\u0026ndash;5719\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003e70 cm\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c2\"\u003e\u003cp\u003eBeta 388872\u0026sup1;\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c3\"\u003e\u003cp\u003ePlant material\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\"\u0026plusmn;\" colname=\"c4\"\u003e\u003cp\u003e8550\u0026thinsp;\u0026plusmn;\u0026thinsp;30\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e\u003cp\u003e9520\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c6\"\u003e\u003cp\u003e9493\u0026ndash;9547\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003e78 cm\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c2\"\u003e\u003cp\u003e18OS/0403\u0026sup3;\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c3\"\u003e\u003cp\u003eBulk organic matter on sediment\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\"\u0026plusmn;\" colname=\"c4\"\u003e\u003cp\u003e8420\u0026thinsp;\u0026plusmn;\u0026thinsp;30\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e\u003cp\u003e9490\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c6\"\u003e\u003cp\u003e9334\u0026ndash;9519\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003e116 cm\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c2\"\u003e\u003cp\u003eBeta 388873\u0026sup1;\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c3\"\u003e\u003cp\u003ePlant material\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\"\u0026plusmn;\" colname=\"c4\"\u003e\u003cp\u003e11430\u0026thinsp;\u0026plusmn;\u0026thinsp;30\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e\u003cp\u003e13200\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c6\"\u003e\u003cp\u003e13255\u0026ndash; 13300\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003e143 cm\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c2\"\u003e\u003cp\u003eBeta 388874\u0026sup1;\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c3\"\u003e\u003cp\u003ePlant material\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\"\u0026plusmn;\" colname=\"c4\"\u003e\u003cp\u003e11270\u0026thinsp;\u0026plusmn;\u0026thinsp;30\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e\u003cp\u003e13130\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c6\"\u003e\u003cp\u003e13058\u0026ndash;13212\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003c/tbody\u003e\u003c/colgroup\u003e\u003c/table\u003e\u003c/div\u003e\u003c/p\u003e\u003cp\u003e\u0026sup1;Radiocarbon Dating Laboratory Beta Analytic; \u0026sup2;Laboratoire de Mesure du Carbone 14; and \u0026sup3; International Chemical Analysis Inc.\u003c/p\u003e\u003cp\u003eThe radiocarbon ages from the Airo 12/01 core indicate sedimentation for the last 13,300 years cal BP (Table\u0026nbsp;\u003cspan refid=\"Tab2\" class=\"InternalRef\"\u003e2\u003c/span\u003e, Fig.\u0026nbsp;\u003cspan refid=\"Fig5\" class=\"InternalRef\"\u003e3\u003c/span\u003e), corresponding to the Holocene, including the Pleistocene/Holocene transition.\u003c/p\u003e\u003cp\u003e\u003c/p\u003e\u003cp\u003eFigure \u003cspan refid=\"Fig5\" class=\"InternalRef\"\u003e3\u003c/span\u003e: Lithological profile, calibrated radiocarbon ages, sedimentation rate, and stratigraphic zones from Lago Airo. The lithology column shows sediment textures and colors with Munsell color notations. Radiocarbon ages (cal yr BP) are plotted against depth (cm) alongside the calibrated age model (solid red line) with confidence intervals (dashed gray lines). Sedimentation rates (cm/yr) are displayed on the right. Four stratigraphic zones (I\u0026ndash;IV) are indicated based on changes in sedimentological characteristics and age-depth relationships.\u003c/p\u003e\u003c/div\u003e\u003cdiv id=\"Sec13\" class=\"Section2\"\u003e\u003ch2\u003e4.2. Grain-size distribution\u003c/h2\u003e\u003cp\u003eAn average percentage of sand-size particles was 79.3\u0026thinsp;\u0026plusmn;\u0026thinsp;30.4% cm with 17.4\u0026thinsp;\u0026plusmn;\u0026thinsp;24.4% of silt and just 3.3\u0026thinsp;\u0026plusmn;\u0026thinsp;12.9% of clay. Considering the coefficient of variation among particle size classes, it is evident that the clay class exhibits the highest variability among the distributions, With a variation coefficient of 393%. Following this, the silt class shows a variability of 140%, and lastly, the sand class displays the least variability at 38.3%.\u003c/p\u003e\u003cp\u003e\u003cb\u003ePhase IV: 13,400 to 11,800 cal yr BP, 146 cm to 93 cm.\u003c/b\u003e In this phase, the sediment was predominantly composed of coarser grains, with sand constituting over 99% of the composition. The distribution of sand displayed low variability (variation coefficient less than 1%). The influx of sand intoLago Airo demonstrated uniformity throughout the designated period, suggesting a phase characterized by low variability and high energy, likely associated with a substantial intensification in the hydrological cycle of the Rio Negro. This period marks the transition between the Pleistocene and Holocene epochs and is globally recognized for its cold conditions, particularly the Younger Dryas, characterized by a rapid and drastic cooling of the climate, especially in the Northern Hemisphere (Rasmussen et al. \u003cspan citationid=\"CR64\" class=\"CitationRef\"\u003e2006\u003c/span\u003e). \u003cb\u003ePhase III: 11,800 to 8,800 cal yr BP, 92 to 68 cm\u003c/b\u003e. This phase shows a significant increase in clays and silt content in sediment and a great variability of sand. The transition from a period characterized by significant transport competence related to extreme climatic events shifts to a phase of substantial variability, as evidenced by a coefficient of variation that was previously less than 1% and has now increased to nearly 30% in terms of sand variability. This aspect indicates a period of intense changes in the lacustrine environment, alternating between extreme events and phases of substantial stability in the lacustrine system, accompanied by significant clay deposition, which was 0.285% in phase 4, increasing to a value of 2% in this phase. In \u003cb\u003ePhase II: 8,800-4,100 cal yr BP, 67 to 38 cm.\u003c/b\u003e The sand sediments show an increase compared to the preceding phase and present a sediment rich in sand with average values of 97.9\u0026thinsp;\u0026plusmn;\u0026thinsp;4.65%. Phase II is characterized by average sand values like Phase IV; however, it exhibits greater variability compared to phase IV, with a variation coefficient of 4.75% against 0.18% in Phase IV. Thus, during the mid-Holocene, a climate shift towards drier conditions in relation to precedent phase is observed, marked by extreme events of sandy sedimentation. \u003cb\u003ePhase I: 4,100 to actual.\u003c/b\u003e Silt became dominant. At the onset of phase I, the Rio Negro became disconnected from Lago Airo, which is suggested by the predominance of silt, associated with low hydrodynamics, and an increase in diatom valve density.\u003c/p\u003e\u003c/div\u003e\u003cdiv id=\"Sec14\" class=\"Section2\"\u003e\u003ch2\u003e4.3. Bulk and stable isotopic organic geochemistry\u003c/h2\u003e\u003cp\u003eThe total organic carbon (TOC) content, with an average of 7.89\u0026thinsp;\u0026plusmn;\u0026thinsp;11.7% for the entire profile, varied from 0.4% at 13,130 cal yr BP (Phase IV) to 41.9% at 780 cal yr BP (Phase 1). The TOC values exhibit increasing mean values across the phases, with concentrations of 1.41% in Phase IV, 2.39% in Phase III, and 3.47% in Phase II. However, a substantial increase is evident in Phase I, with mean values reaching 28%, indicating the consolidation of a lentic environment.\u003c/p\u003e\u003cp\u003eThe C/N ratio was greater than 20 throughout Airo 12/01 core with an average value of 47.8\u0026thinsp;\u0026plusmn;\u0026thinsp;12.9 indicating a huge influence of terrestrial organic matter, with a strong degradation component. The C/N ratio varying between 4 and 10 (~\u0026thinsp;8) commonly suggests an algae source, whereas C/N ratios greater than 20 are attributed to an origin of macrophytic margin vegetation or terrestrial plant source (Meyers \u003cspan citationid=\"CR52\" class=\"CitationRef\"\u003e2003\u003c/span\u003e).\u003c/p\u003e\u003cp\u003eA trend towards an increase in the C/N ratio is observed from Phases IV to II, ranging between 49.3\u0026thinsp;\u0026plusmn;\u0026thinsp;9.58 during Phase IV, 52.6\u0026thinsp;\u0026plusmn;\u0026thinsp;7.08 during Phase III, and 58.1\u0026thinsp;\u0026plusmn;\u0026thinsp;5.70 during Phase II, likely indicating a period when autochthonous influence was reduced because of a regional dry phase (Nascimento et al. \u003cspan citationid=\"CR60\" class=\"CitationRef\"\u003e2021\u003c/span\u003e). An abrupt decrease in the C/N ratio values is observed during Phase I, with an average of 35.5\u0026thinsp;\u0026plusmn;\u0026thinsp;7.67. This denotes a significant increase in autochthonous influence, as evidenced by high values of sedimentary chlorophyll.\u003c/p\u003e\u003cp\u003e\u003cb\u003eIn Phase IV (ca. 13,400 to 11,800 cal yr BP), geochemical\u003c/b\u003e data show a TOC average of 2.2%, high C/N ratios (average\u0026thinsp;=\u0026thinsp;52.1), and low chlorophyll derivatives (average\u0026thinsp;=\u0026thinsp;5.6 SPDU). δ15N values suggest a source related to atmospheric nitrogen fixation, likely due to the extremely oligotrophic nature of the system. These results suggest that Phase IV represented Lago Airo as a lotic-like ecosystem with strong connections to the Rio Negro. The δ15N values approaching 0\u0026permil; support the presence of predominantly terrestrial organic material. The loss of nitrogen induces that this organic matter is poor in Nitrogen favoring the biological process of nitrogen fixation (references) attested by δ15N values near 0\u0026permil;. Negative values, particularly evident in Phase IV, suggest significant decomposition processes of the organic material, likely due to prolonged exposure in the podzolized soils prevalent in the Rio Negro basin. \u003cb\u003eIn Phase III (11,800 to 8,800 cal yr BP)\u003c/b\u003e, exhibiting substantial variability. \u003cb\u003eT\u003c/b\u003ehere was a significant increase in clay content, rising from 0.256% in Phase IV to 2.05%, though the sediments remained sandy-textured overall. TOC values increased slightly to 2.39%, and higher C/N ratios indicated the transport of detrital organic matter from the surrounding basin. \u003cb\u003ePhase II (8,800 to 4,100 cal yr BP)\u003c/b\u003e there was a decrease in clay content (to 0.299%) alongside a progressive increase in TOC, reaching 3.47%. During this phase Chlorophyll derivatives values around 5,501 to 20.2 SPDU, showed lake-like conditions as well as the information given by C/N (Fig.\u0026nbsp;\u003cspan refid=\"Fig5\" class=\"InternalRef\"\u003e3\u003c/span\u003e). Higher C/N ratios continued to reflect detrital organic matter input. This phase marks the onset of the disconnection between Lago Airo and the Rio Negro and aligns with broader mid-Holocene dry conditions, with decreasing lake levels observed across Amazonia. Lago Airo\u0026rsquo;s ecosystem showed evidence of reduced fluvial connectivity and higher external organic input, consistent with high C/N ratios, and reflects progressive ecological isolation. \u003cb\u003ePhase I (4,100 cal yr BP to present)\u003c/b\u003e Sediments transitioned to being predominantly silt (average\u0026thinsp;=\u0026thinsp;59.1%) with an increase in clay (average\u0026thinsp;=\u0026thinsp;10.2%). This phase also saw a pronounced increase in autochthonous production, as evidenced by rising TOC values (up to 25.0%) and chlorophyll derivatives (average\u0026thinsp;=\u0026thinsp;12.7 SPDU). These changes suggest an increase in mean water level, likely driven by higher precipitation associated with the South American Monsoon System. The shift to finer sediments, coupled with increased diatom density and autochthonous production, highlights the establishment of a lentic system. This phase represents the wettest conditions in Amazonia over the past millennia, favoring ecosystem stabilization, higher productivity, and organic matter accumulation in Lago Airo.\u003c/p\u003e\u003cp\u003e\u003c/p\u003e\u003c/div\u003e\u003cdiv id=\"Sec15\" class=\"Section2\"\u003e\u003ch2\u003e4.4. Diatoms Assemblage\u003c/h2\u003e\u003cp\u003eIt was identified 73 taxa. The 10 most frequent were \u003cem\u003eEunotia hirudo\u003c/em\u003e Metzeltin and Lange-Bertalot (mean 20.6\u0026thinsp;\u0026plusmn;\u0026thinsp;21.4%), \u003cem\u003eAulacoseira distans\u003c/em\u003e (Ehrenberg) Simonsen (12.3\u0026thinsp;\u0026plusmn;\u0026thinsp;14.3%), \u003cem\u003eEunotia didyma\u003c/em\u003e Grunow \u003cem\u003evar. didyma\u003c/em\u003e (7.6\u0026thinsp;\u0026plusmn;\u0026thinsp;12.6%), \u003cem\u003eAulacoseira\u003c/em\u003e sp. 3 (6.4\u0026thinsp;\u0026plusmn;\u0026thinsp;12.1%), \u003cem\u003eAulacoseira\u003c/em\u003e cf. \u003cem\u003ecalypsi\u003c/em\u003e Tremarin, Torgan et Ludwig (3.4% \u0026plusmn; 11.1%), \u003cem\u003eEunotia ventriosa\u003c/em\u003e Patrick (3.2%), \u003cem\u003eEunotia bilunaris\u003c/em\u003e (Ehenberg) Schaarschmidt (3.1\u0026thinsp;\u0026plusmn;\u0026thinsp;4.7%), \u003cem\u003eEunotia gibbosa\u003c/em\u003e Grunow in Van Heurck (2.9% \u0026plusmn; 4.4%), \u003cem\u003eAulacoseira granulata\u003c/em\u003e (Ehrenberg\u003cem\u003e)\u003c/em\u003e Simonsen (2.8\u0026thinsp;\u0026plusmn;\u0026thinsp;3.7%), \u003cem\u003eEunotia floweri\u003c/em\u003e Metzeltin and Lange-Bertalot (2.7\u0026thinsp;\u0026plusmn;\u0026thinsp;6.2%). Figure\u0026nbsp;4 shows diatom diagram.\u003c/p\u003e\u003cp\u003eThe diatoms density attained a peak (37,542 valves/g) around 10k cal. years BP (79 cm). \u003cem\u003eEunotia hirudo, Eunotia bilunaris\u003c/em\u003e, and \u003cem\u003eEunotia floweri\u003c/em\u003e were highly abundant during phases PIV, PIII, and PII. Phase II was marked with the disappearance of \u003cem\u003eAulacoseira\u003c/em\u003e cf. \u003cem\u003ecalypsi\u003c/em\u003e from records and a small decrease of \u003cem\u003eEunotia hirudo\u003c/em\u003e and \u003cem\u003eEunotia bilunaris\u003c/em\u003e. Phase I recorded the highest diatom densities, with average of 5.698,183 valves/g. Planktonic species dominated this phase with \u003cem\u003eAulacoseira distans\u003c/em\u003e and \u003cem\u003eA. granulata\u003c/em\u003e, while \u003cem\u003eA.\u003c/em\u003e cf. \u003cem\u003ecalypsi\u003c/em\u003e reappeared on record and \u003cem\u003eEunotia floweri\u003c/em\u003e abundance decreased.\u003c/p\u003e\u003cp\u003e\u003cb\u003ePhase IV, from ca. 13,360 (145 cm) to 11,800 cal yr BP (92 cm).\u003c/b\u003e Diatoms valves density was relatively low (i.e., average\u0026thinsp;=\u0026thinsp;1,684 valves/g\u0026thinsp;\u003cb\u003e\u0026plusmn;\u003c/b\u003e\u0026thinsp;1,800; CV\u0026thinsp;=\u0026thinsp;107%; minimum of 88 and maximum of 7,337) with mainly periphytic taxa. Sediments was dominated by coarser sediments (sand represents 99,7%). Geochemical data shows a TOC average of 1.4% \u003cb\u003e\u0026plusmn;\u003c/b\u003e 0,8; CV\u0026thinsp;=\u0026thinsp;56,1%; minimum of 0.40% and maximum of 3.54%. C/N ratio presents high values (average\u0026thinsp;=\u0026thinsp;50.3\u0026thinsp;\u003cb\u003e\u0026plusmn;\u003c/b\u003e\u0026thinsp;10.1; CV\u0026thinsp;=\u0026thinsp;20.1%; minimum of 26.1 and maximum of 71.6., Chlorophyll derivatives are presents low values with average of 2.93\u0026thinsp;\u003cb\u003e\u0026plusmn;\u003c/b\u003e\u0026thinsp;1.6; CV\u0026thinsp;=\u0026thinsp;54,3%; minimum of 0,76 SPDU and maximum of 6.12 SPDU. \u003csup\u003e15\u003c/sup\u003eN values show a source related to a nitrogen atmospheric fixation metabolism, probably due to the extremely oligotrophic nature of the system. Our results suggest that Phase IV represented the Lago Airo as a lotic-like ecosystem with connections with the Rio Negro, characterized by high energy and thereby low diatom sedimentation.\u003c/p\u003e\u003cp\u003e\u003cb\u003ePhase III from ca. 11,800 (145 cm) \u0026ndash; 8,800 cal yr BP (68 cm).\u003c/b\u003e Diatoms valves density was relatively low (i.e., average\u0026thinsp;=\u0026thinsp;5,589 valves/g\u0026thinsp;\u003cb\u003e\u0026plusmn;\u003c/b\u003e\u0026thinsp;9,455; CV\u0026thinsp;=\u0026thinsp;107%; minimum of 0 and maximum of 37,543 valves/g) with mainly periphytic taxa. This phase was dominated by coarse sediments (sand represents 93,3%) and showed a progressive increase in TOC compared to the previous phase, reaching an average value of 2.39%, with a variation coefficient around 52.4%; minimum of 0,7 and maximum of 5.3%. In this phase was observed an increase in the C/N ratio denoted the increase transport of detrital organic matter from the basins. The C/N ratio presents high values of 54.6\u0026thinsp;\u003cb\u003e\u0026plusmn;\u003c/b\u003e\u0026thinsp;7.1 and \u003cb\u003ea\u003c/b\u003e variation coefficient with low variability, around 13,5%; a minimum of 33.1 and a maximum of 66.6. The increase in the C/N ratio denotes the transport of detrital organic matter from the basins. Chlorophyll derivatives present low values with an average of 5.86\u0026thinsp;\u003cb\u003e\u0026plusmn;\u003c/b\u003e\u0026thinsp;2.00 SPDU; CV\u0026thinsp;=\u0026thinsp;26,6%; minimum of 2.14 SPDU and maximum of 11.9 SPDU.\u003c/p\u003e\u003cp\u003e\u003cb\u003ePhase II: 8,800 (67 cm) to 4,100 (38 cm) cal yr BP\u003c/b\u003e. Diatoms valves density decrease in relation to the precedent phase (i.e., average\u0026thinsp;=\u0026thinsp;1,454 valves/g\u0026thinsp;\u003cb\u003e\u0026plusmn;\u003c/b\u003e\u0026thinsp;1,383; CV\u0026thinsp;=\u0026thinsp;95%; minimum of 0 and maximum of 4,742 valves/g) with mainly periphytic taxa. Sediments was also dominated by coarse sediments (sand represent 97,9%). Geochemical data shows a TOC average of 3.47% \u003cb\u003e\u0026plusmn;\u003c/b\u003e 1.7%; CV\u0026thinsp;=\u0026thinsp;49.7%; minimum of 1,36 and maximum of 5,3. The C/N ratio presents high values (average\u0026thinsp;=\u0026thinsp;58,1\u0026thinsp;\u003cb\u003e\u0026plusmn;\u003c/b\u003e\u0026thinsp;5,7; CV\u0026thinsp;=\u0026thinsp;9,81%; minimum of 48,6 and maximum of 70.8), and chlorophyll derivatives presents low values (average\u0026thinsp;=\u0026thinsp;5,86\u0026thinsp;\u003cb\u003e\u0026plusmn;\u003c/b\u003e\u0026thinsp;3.74 SPDU; CV\u0026thinsp;=\u0026thinsp;63,9%; minimum of 2,1 SPDU and maximum of 11,9 SPDU).\u003c/p\u003e\u003cp\u003e\u003cb\u003ePhase I: From ca. 4,100 cal yr BP (37 cm) to actual.\u003c/b\u003e Here we saw a change in diatom structure, density, and composition, marking a transition from a lotic system to a lentic one. Diatom density increased (i.e., average\u0026thinsp;=\u0026thinsp;4.69 x10\u003csup\u003e6\u003c/sup\u003e valves/g\u0026thinsp;\u003cb\u003e\u0026plusmn;\u003c/b\u003e\u0026thinsp;4.15; CV\u0026thinsp;=\u0026thinsp;88%; minimum of 2.01 x10\u003csup\u003e3\u003c/sup\u003e valves/g and maximum of 14.1 x10\u003csup\u003e6\u003c/sup\u003e valves/g). These diatom communities were primarily composed of planktonic taxa. After approximately 4,100 cal yr BP there was a shift towards silt dominance in the sediment composition, with an average of 65.7\u0026thinsp;\u0026plusmn;\u0026thinsp;30.4% (coefficient of variation\u0026thinsp;=\u0026thinsp;46.3%), ranging from a minimum of 0.6% to a maximum of 93.0%, followed by clay\u0026thinsp;=\u0026thinsp;11,1\u0026thinsp;\u0026plusmn;\u0026thinsp;6,72%; CV\u0026thinsp;=\u0026thinsp;60,1%; minimum of 0 and maximum of 20,3). The heightened contribution of silt and a noticeable decrease in C/N ratios, averaging 34.9\u0026thinsp;\u0026plusmn;\u0026thinsp;7.7 (coefficient of variation\u0026thinsp;=\u0026thinsp;21.9%), suggest a diminishing influence of the Rio Negro on Lago Airo and an increase in autochthonous production. This is evidenced by the rise in total organic carbon (TOC) content, which increased from 3.4% in Phase II to 25.0%. A substantial increase in chlorophyll derivative reaching was simultaneous reinforces the formation of a lacustrine environment with reduced influence from the Rio Negro, as we can see on diatom data.\u003c/p\u003e\u003cp\u003e\u003c/p\u003e\u003cp\u003eThe planktonic-benthic ratio considered the mean relative abundance of the most abundant and significant species. All planktonic species were \u003cem\u003eAulacoseira\u003c/em\u003e spp. and the benthics were \u003cem\u003eEunotia\u003c/em\u003e spp. The P:B ratio also maintained the characteristic of two main phases formed by phases IV, III and II which is characterized by diatom low density and greater representation of \u003cem\u003eEunotia\u003c/em\u003e spp as opposed to phase I, which presents a high density of diatoms and the predominance of \u003cem\u003eAulacoseira\u003c/em\u003e spp.\u003c/p\u003e\u003cp\u003e\u003c/p\u003e\u003cp\u003eA direct relationship can be observed among the alterations in the structure ofLago Airo diatom community, as represented by the first factor in the principal component analysis and their abundance, which is assessed based on cell concentration in the sediment. Factor 1 serves as a principal component considering all species, indicating a transition from a benthic to a planktonic community. These changes in the diatom community precede modifications in diatom biomass, primarily resulting from the establishment of a more productive lentic environment. The abrupt shift between basal phases PIV, PIII and PII and phase 1 appears to be primarily preceded by a transformation in community structure, particularly evident from 4100 cal yr BP. Substantial shifts in diatom biomass become noticeable from 3660 cal yr BP onward.\u003c/p\u003e\u003cp\u003e\u003c/p\u003e\u003c/div\u003e"},{"header":"5. Discussion","content":"\u003cdiv id=\"Sec17\" class=\"Section2\"\u003e\u003ch2\u003e5.1. Paleolimnological interpretations\u003c/h2\u003e\u003cp\u003eLago Airo records showed consequences of climate change on the Rio Negro basin and reveals a clear separation between the earlier high-energy, lotic system and the more recent low-energy, lentic conditions (Figs.\u0026nbsp;\u003cspan refid=\"Fig2\" class=\"InternalRef\"\u003e1\u003c/span\u003e and \u003cspan refid=\"Fig4\" class=\"InternalRef\"\u003e2\u003c/span\u003e supplementary materials), reflecting high amplitude of hydrological and climatic changes in Lago Airo.\u003c/p\u003e\u003cp\u003eThe paleoclimate evidence is discussed for the Younger Dryas, Late Holocene and Early-Mid Holocene. The Airo 12/01 core first phase coincides with the B\u0026oslash;lling-Aller\u0026oslash;d (15\u0026thinsp;\u0026minus;\u0026thinsp;13 ka yrs) end, a warm period of the high latitude North Atlantic region. Its formation coincides with the significant increase in the level of Lagoa da Pata recorded on the LPT V core mainly after 14,500 cal yr BP (Cordeiro et al. \u003cspan citationid=\"CR25\" class=\"CitationRef\"\u003e2011\u003c/span\u003e).\u003c/p\u003e\u003cp\u003e\u003cb\u003eSince about 14,000 to 4,000 cal yr BP\u003c/b\u003e, the Rio Negro has behaved as a progradational system, infilling in downstream series a sequence of structurally controlled sedimentary basins or \u0026lsquo;compartments,\u0026rsquo; creating alluvial floodplains and associated anabranching channel systems (Latrubesse and Franzinelli \u003cspan citationid=\"CR39\" class=\"CitationRef\"\u003e2005\u003c/span\u003e). The Lago Airo seems to be part of this dynamic. About 750 km northeast of Lago Airo, in a region now dominated by cerrado vegetation (Brazilian classification), an integrative study of sediment cores from three lakes (Beltran et al. \u003cspan citationid=\"CR12\" class=\"CitationRef\"\u003e2023\u003c/span\u003e) indicated savannah type ecosystems that have remained stable over the entire Holocene period, with minimal changes in vegetation. Lake sediment records indicate sensitivity to local water table fluctuations.\u003c/p\u003e\u003cp\u003e\u003cb\u003eDuring Phase IV (13,360\u0026ndash;11,800 cal yr BP)\u003c/b\u003e, Lago Airo functioned as a lotic-like ecosystem connected to the Rio Negro. This phase was characterized by high-energy conditions and low diatom sedimentation. The low nutrient levels and high C/N ratio indicate an environment with limited organic matter input and reduced primary production, likely due to the high-energy, flowing water dynamics of the system. Around 13,400 cal yr BP the lake exhibited higher sediment deposition which may be explained by the contemporaneous high precipitation during and just after the last deglaciation. In the upper Rio Negro River at Lagoa da Pata, after 15,500 cal yr BP until 10,000 cal yr BP occurred a substantial increase in lake level attested by increase phytoplanktonic organic matter (Cordeiro et al. \u003cspan citationid=\"CR25\" class=\"CitationRef\"\u003e2011\u003c/span\u003e).\u003c/p\u003e\u003cp\u003e\u003cb\u003eDuring Phase III (11,800\u0026ndash;8,800 cal yr BP)\u003c/b\u003e, the sand percentage decreased, while higher proportions of silt and clay were observed (Fig.\u0026nbsp;4). These grain size variations reflect changes in the system's hydraulic energy, with reduced sand deposition indicating a decline in fluvial influence. This phase also shows a progressive increase in TOC and a high C/N ratio, suggesting enhanced transport of detrital organic matter from the surrounding basins. While the environment remained lotic, it exhibited signs of increased organic input and possibly higher productivity compared to Phase IV. In Acarabixi Lake (Rodr\u0026iacute;guez-Zorro et al. \u003cspan citationid=\"CR66\" class=\"CitationRef\"\u003e2017\u003c/span\u003e) during the early Holocene, a highly dynamic floodplain lake in which forest vegetation occurred with direct influence by a channel of the Rio Negro from 10,840 to 8240 cal yr BP. According to Rodr\u0026iacute;guez-Zorro et al. (\u003cspan citationid=\"CR66\" class=\"CitationRef\"\u003e2017\u003c/span\u003e), Rio Negro C/N ratios are particularly high considering the record obtained in Acarabixi, due to the influence of detritic organic matter and humic acids, highly deficient in nitrogen. The high C/N ratios found on Lago Airo reveal also a great influence of Rio Negro over the lake at that specific time. Similarly, high C/N ratio values were found on Acarabixi lake records, situated nearby (Rodr\u0026iacute;guez-Zorro et al. \u003cspan citationid=\"CR66\" class=\"CitationRef\"\u003e2017\u003c/span\u003e). In Roraima, North hemisphere Equatorial zone with Cerrado vegetation, Lake Caracaran\u0026atilde; was formed around 11,500 cal yr BP (Sim\u0026otilde;es Filho \u003cspan citationid=\"CR72\" class=\"CitationRef\"\u003e2000\u003c/span\u003e, Cordeiro et al. \u003cspan citationid=\"CR24\" class=\"CitationRef\"\u003e2014\u003c/span\u003e, Beltran et al. \u003cspan citationid=\"CR12\" class=\"CitationRef\"\u003e2023\u003c/span\u003e) with evidence of dry periods around 10,000-7800 cal yr BP (Sim\u0026otilde;es Filho \u003cspan citationid=\"CR72\" class=\"CitationRef\"\u003e2000\u003c/span\u003e, Cordeiro et al. \u003cspan citationid=\"CR24\" class=\"CitationRef\"\u003e2014\u003c/span\u003e, Beltran et al. \u003cspan citationid=\"CR12\" class=\"CitationRef\"\u003e2023\u003c/span\u003e). This phase was characterized by relatively low diatom density (valves/g) with mainly epiphytic taxa specifically \u003cem\u003eEunotia\u003c/em\u003e spp. (\u003cem\u003eE. hirudo, E. bilunaris\u003c/em\u003e, and \u003cem\u003eE. flowerii\u003c/em\u003e). This genus occurs on acid, oligotrophic/distrophic and freshwater environments (Round et al. \u003cspan citationid=\"CR67\" class=\"CitationRef\"\u003e1990\u003c/span\u003e, Spaulding et al. \u003cspan citationid=\"CR74\" class=\"CitationRef\"\u003e2021\u003c/span\u003e), which explains its high occurrence in Lago Airo. The presence of \u003cem\u003eAulacoseira distans\u003c/em\u003e and \u003cem\u003eA. granulata\u003c/em\u003e indicates that these species have ecological flexibility. While they are primarily limnophilic and prefer still water environments, their occurrence in flowing water suggests they can adapt to dynamic hydrological conditions (Moro and F\u0026uuml;rstenberger, \u003cspan citationid=\"CR59\" class=\"CitationRef\"\u003e1997\u003c/span\u003e)\u003c/p\u003e\u003cp\u003e\u003cb\u003ePhase II (8,800\u0026ndash;4,100 cal yr BP)\u003c/b\u003e is characterized by continued high-energy conditions, with coarse sediments predominating. The high C/N ratios and elevated TOC levels suggest a substantial contribution of detrital organic matter, indicating increased external organic input but limited primary production. This phase corresponds to the mid-Holocene dry period (Mayle and Power, \u003cspan citationid=\"CR48\" class=\"CitationRef\"\u003e2008\u003c/span\u003e), during which many Amazonian sites experienced a decline in lake levels (Cordeiro et al. \u003cspan citationid=\"CR21\" class=\"CitationRef\"\u003e1997\u003c/span\u003e, 2008) and a reduction in forest biomass (Bush et al. \u003cspan citationid=\"CR15\" class=\"CitationRef\"\u003e2007\u003c/span\u003e, Behling et al. \u003cspan citationid=\"CR10\" class=\"CitationRef\"\u003e2001\u003c/span\u003e, Absy et al. \u003cspan citationid=\"CR1\" class=\"CitationRef\"\u003e1991\u003c/span\u003e, Fontes et al. \u003cspan citationid=\"CR32\" class=\"CitationRef\"\u003e2017\u003c/span\u003e). In Lago Airo it is noted that at 8,000 cal yr BP there is an abrupt decrease in the density of diatom valves with subsequent variability and a substantial decrease until approximately 3,700 cal yr BP. A study carried out by Bush and Colinvaux (\u003cspan citationid=\"CR14\" class=\"CitationRef\"\u003e1988\u003c/span\u003e) on lake sediment in Ecuador (Lago Ayauch, Ecuador (500 m) showed horizons of laminated sediment and weathered gyttja (lake sediment rich in organic matter), showing a reduction in the water level in the period 4,200-3,150 years B.P., confirmed by the absence of open water diatoms and the abundance of sponge spicules. The resumption of gyttja deposition around 3,150 years B.P. indicated that the lake level, and presumably rainfall, had begun to increase\u003c/p\u003e\u003cp\u003e\u003cb\u003eAt phase I (ca 4,100 cal yr BP to present)\u003c/b\u003e the Rio Negro disconnects from Lago Airo, as suggested by the predominance of silt and clay, indicating a transition between a predominantly erosive environment (lotic-like condition) to a lower energy environment, producing a lentic ecosystem on Lago Airo. There is also a significant decrease on C/N ratio, which indicates a diminishing of the river influence over the lake. Analysis of Corg/Ntotal atomic ratio, δ\u003csup\u003e13\u003c/sup\u003eC and δ\u003csup\u003e15\u003c/sup\u003eN of organic matter can allow distinguishing between algae and vascular plant contributions to sediment organic matter (Meyers and Ishiwatari \u003cspan citationid=\"CR53\" class=\"CitationRef\"\u003e1993\u003c/span\u003e, Meyers \u003cspan citationid=\"CR52\" class=\"CitationRef\"\u003e2003\u003c/span\u003e). The higher δ\u003csup\u003e15\u003c/sup\u003eN rates during phase II additionally with the low values of C/N ratio, suggest the development of lacustrine productivity in agreement with the rising of silt and clay grain size on records. It was also recorded the highest diatom density during this phase, with planktonic species overcoming, such as \u003cem\u003eAulacoseira distans\u003c/em\u003e and \u003cem\u003eA. granulata\u003c/em\u003e, whereas \u003cem\u003eAulacoseira cf. calypsi\u003c/em\u003e reappeared on record. \u003cem\u003eAulacoseira\u003c/em\u003e is the most representative genera in Lago Airo diatoms composition.\u003c/p\u003e\u003cp\u003eThis phase marks a transition from a lotic to a lentic system. The increase in diatom density, shift to finer sediments, and higher TOC content reflect a more stable, low-energy environment with higher primary production. The lower C/N ratio indicates increased autochthonous production, suggesting diminished influence from the Rio Negro and the development of a lacustrine ecosystem.\u003c/p\u003e\u003cp\u003e\u003cem\u003eAulacoseira\u003c/em\u003e spp. are a good indicator of turbulent waters, because its heavy frustules require high water movement to remain on the photic zone (Wolin and Stone \u003cspan citationid=\"CR78\" class=\"CitationRef\"\u003e2010\u003c/span\u003e). The dominance of \u003cem\u003eAulacoseira\u003c/em\u003e spp. indicated a lentic system on phase I after lateral displacement of the Rio Negro main channel or decrease of Rio Negro water level forming a shallow lake on Lago Airo region. This phase is considered the wettest in Amazonia principally after 3,000 cal yr BP, when the climate becomes progressively more humid and probably with less intranual hydric variability. This condition favoring the installation of a slower environment of greater productivity and potency of organic matter accumulation. Analogous to Lago Airo area, the Acarabix\u0026iacute; lake (00\u0026ordm;20' S; 64\u0026ordm;29' O) located at a fluvial plain of the Rio Negro (AM) demonstrated lateral displacement of the main channels of the river throughout time (Rodriguez -Zorro et al. 2017).\u003c/p\u003e\u003cp\u003eDuring the Late Holocene, according to Helama et al. (\u003cspan citationid=\"CR34\" class=\"CitationRef\"\u003e2017\u003c/span\u003e) the \u0026lsquo;Dark Ages Cold Period\u0026rsquo; (DACP) is probably the climate anomaly most frequently discussed of the first millennium. Also, the Medieval Climate Anomaly (MCA, ca. 950\u0026ndash;1200 \u003cem\u003eAD\u003c/em\u003e) and the Little Ice Age (LIA, ca. 1400\u0026ndash;1700 \u003cem\u003eAC\u003c/em\u003e) are important climate events that occurred during the Late Holocene (Mann et al. \u003cspan citationid=\"CR43\" class=\"CitationRef\"\u003e2009\u003c/span\u003e). The MCA is considered the more recent warm interval from the pre-industrial period and had a great influence on US and Europe climate. Nevertheless, the causes of this climate anomaly are uncertain (Mann \u003cspan citationid=\"CR44\" class=\"CitationRef\"\u003e2002\u003c/span\u003e). On the opposite, the most recent and longer episode of glacier advance and temperature drop throughout the Holocene is registered at the Little Ice Age with a global scale effect (Chambers et al. \u003cspan citationid=\"CR18\" class=\"CitationRef\"\u003e2014\u003c/span\u003e). A climate simulation model confirmed the global scale effects of both climate events (Mann et al. \u003cspan citationid=\"CR43\" class=\"CitationRef\"\u003e2009\u003c/span\u003e). Here we discuss these climatic events at the Rio Negro Basin (and Amazon Basin overall) and the possible forcings behind these paleoclimate periods.\u003c/p\u003e\u003cdiv id=\"Sec18\" class=\"Section3\"\u003e\u003ch2\u003e5.1.1. The Younger Dryas\u003c/h2\u003e\u003cp\u003eThe Younger Dryas was an abrupt and global climate event that occurred in ca. 12.9\u0026ndash;11.7 ka (Cohen et al. \u003cspan citationid=\"CR19\" class=\"CitationRef\"\u003e2018\u003c/span\u003e). Meanwhile, during the same period, there is evidence of the Atlantic Meridional Overturning (AMOC) weakening (MacManus et al. 2004). AMOC reduction would have been responsible for cooling in the Northern Hemisphere and warming in the Southern Hemisphere by moving the ITCZ to South (Carlson \u003cspan citationid=\"CR17\" class=\"CitationRef\"\u003e2010\u003c/span\u003e). In the Southern Hemisphere, the temperature in Antarctica showed warming at the Early Holocene period (between 11,000- and 9,000-years BP) (Masson et al. \u003cspan citationid=\"CR46\" class=\"CitationRef\"\u003e2000\u003c/span\u003e).\u003c/p\u003e\u003cp\u003eCariaco basin, on Venezuela, showed a shift from a warm and wet to a cold and dry condition at YD (Lea et al. \u003cspan citationid=\"CR40\" class=\"CitationRef\"\u003e2003\u003c/span\u003e) and authors believe that there was a southward shift of the ITCZ during the period. Concurrent, Lake Titicaca indicated maximum water level (Baker et al. \u003cspan citationid=\"CR4\" class=\"CitationRef\"\u003e2001\u003c/span\u003e). The results obtained in Lago Airo showed evidence that during YD climatic conditions in the region were more humid. The lowest average TOC (1.34%) as recorded. As seen previously, the low TOC concentration is related to high hydrodynamic energy, which may represent a strong fluvial influence at the lake.\u003c/p\u003e\u003c/div\u003e\u003cdiv id=\"Sec19\" class=\"Section3\"\u003e\u003ch2\u003e5.1.2. Early (11,700- 8,200 Years BP) to Mid Holocene (8,200\u0026ndash;4,200 Years BP)\u003c/h2\u003e\u003cp\u003eThe period of the early-mid-Holocene represented an intermediary climate condition in records all over the Amazon region (De Freitas et al. \u003cspan citationid=\"CR27\" class=\"CitationRef\"\u003e2001\u003c/span\u003e, Moreira et al. \u003cspan citationid=\"CR55\" class=\"CitationRef\"\u003e2013a\u003c/span\u003e, Cordeiro et al. 2008, \u003cspan citationid=\"CR24\" class=\"CitationRef\"\u003e2014\u003c/span\u003e). Chemical analysis of Lake Valencia, Venezuela (Lewis and Webezahn \u003cspan citationid=\"CR42\" class=\"CitationRef\"\u003e1981\u003c/span\u003e) showed that after the last glacial maximum the lake was ephemeral until 10,500 years B.P. From then on there was a decrease in salinity associated with a wet period in 8,000 years B.P. From 8,000 years B.P. to 7,500 years B.P. salinity increased suggesting a period of intensified dry conditions. Clayey clastic material was deposited between 7,400 and 6,000 years B.P. with low levels of sedimentary chlorophyll suggesting a return to dry conditions. Pollen analysis in the same lake showed changes in vegetation after the last glacial period, with forest-semideciduous vegetation appearing between 9,800 and 8,300 years B.P. (Leyden \u003cspan citationid=\"CR41\" class=\"CitationRef\"\u003e1985\u003c/span\u003e). Between 8,300 and 5,200 years B.P. there was an expansion in savannah vegetation. In Lake Acarabixi, middle Rio Negro basin, a gap of sedimentation was observed between 8,200\u0026ndash;1,500 cal yrs B.P. after a substantial decrease in carbon rates that coincides with the dry phase of the Holocene in Caraj\u0026aacute;s and low water events in Roraima and Humait\u0026aacute; (Cordeiro et al. 2008).\u003c/p\u003e\u003cp\u003eBetween 8,700 and 5,800 cal yr BP, it was observed dry events in Ecuador, western Amazon (Weng et al. \u003cspan citationid=\"CR76\" class=\"CitationRef\"\u003e2002\u003c/span\u003e). Concurrently, the highest frequency of forest fires in the Amazon region were recorded for ca. 8,000\u0026ndash;4,000 cal yr BP (Cordeiro et al. \u003cspan citationid=\"CR24\" class=\"CitationRef\"\u003e2014\u003c/span\u003e). A middle Holocene dry event was also documented in the Peruvian Amazon that appears to have lasted from ca. 7,200 cal yr BP until ca. 3,300 cal yr BP (Bush et al. \u003cspan citationid=\"CR15\" class=\"CitationRef\"\u003e2007\u003c/span\u003e). Climate simulations detected an intensification of seasonal winds at this period, which may have caused the diminishing of wind convergence upon the Amazonian region and this condition might explain the precipitation rates\u0026rsquo; drop in this region during this time (Melo and Marengo \u003cspan citationid=\"CR50\" class=\"CitationRef\"\u003e2008\u003c/span\u003e).\u003c/p\u003e\u003cp\u003eOn early-mid-Holocene, the Lago Airo area showed low diatoms density, however at some periods presenting peaks of planktonic diatoms. These peaks on ca. 10,930 cal yr BP and 9,100 could represent a formation of a lentic system, intervals in which the Rio Negro disconnected of Lago Airo area, at a period when the Rio Negro connection with Lago Airo Area was strong. The records of the early-mid-Holocene on Lago Airo area represented a mainly lotic phase inferred by coarse grains results.\u003c/p\u003e\u003c/div\u003e\u003cdiv id=\"Sec20\" class=\"Section3\"\u003e\u003ch2\u003e5.1.3. Late Holocene (4,200 Years BP to present).\u003c/h2\u003e\u003cp\u003eDuring the late Holocene, the Amazon region was more humid than early mid-Holocene (Moreira et. al. \u003cspan citationid=\"CR55\" class=\"CitationRef\"\u003e2013a\u003c/span\u003e). We interpreted the possible isolation of Lago Airo area from the Rio Negro as an indicative of a wetter condition, where highly dense vegetation might have isolated the connection with the river and formed a lake as is currently observed. This suggestion can be reinforced by the diminished C/N ratio and sand concentration. With lake isolation, the organic matter was less diluted, so the TOC presents high values during this period.\u003c/p\u003e\u003cp\u003eA wet condition in the Late Holocene was also registered at several regions at the Amazon rainforest (Fig.\u0026nbsp;\u003cspan refid=\"Fig9\" class=\"InternalRef\"\u003e8\u003c/span\u003e): Mayle et al. (\u003cspan citationid=\"CR47\" class=\"CitationRef\"\u003e2000\u003c/span\u003e) reported that humid evergreen rainforests on the southern margin of Amazonia expanded due to increased precipitation after 2790 cal yr BP. Similarly, increased lacustrine productivity was observed in the Caraj\u0026aacute;s region (~\u0026thinsp;2800\u0026ndash;1300 cal yr BP) at 800 m elevation outside the Amazon Basin's floodplain (Cordeiro et al., 2008), indicated by higher chlorophyll derivatives and TOC accumulation rates. Comparable trends were reported in the eastern Colombian Amazon (Behling and Hooghiemstra, \u003cspan citationid=\"CR9\" class=\"CitationRef\"\u003e2000\u003c/span\u003e), and central Amazon (Behling et al., \u003cspan citationid=\"CR10\" class=\"CitationRef\"\u003e2001\u003c/span\u003e). Some authors indicate that this condition was linked with the northward shift of ITCZ position (Behling and Hooghimiestra 2000, Silva Dias et al. \u003cspan citationid=\"CR70\" class=\"CitationRef\"\u003e2009\u003c/span\u003e).\u003c/p\u003e\u003cp\u003eIn Calado Lake, 80 km from the confluence of the Amazonas River and the Rio Negro, Behling et al. (\u003cspan citationid=\"CR10\" class=\"CitationRef\"\u003e2001\u003c/span\u003e) found longer periods of high water in 4,070 cal yr BP. In the Llanos Orientales, Behling and Hooghiemstra (\u003cspan citationid=\"CR9\" class=\"CitationRef\"\u003e2000\u003c/span\u003e) found wetter climatic conditions after ca. 3,500-year BP, when according to them, grassland savanna decreased. In Lago Airo area a true-lake formation was inferred since the last ca. 2000 years due to more frequent isolation events of Lago Airo from the Rio Negro. In Lake Marac\u0026aacute; in eastern Amazonia Sedimentation resumed (~\u0026thinsp;3600\u0026ndash;2700 cal yr BP) with reduced Amazon River inflow. Full hydrological connection with the river was established by 1880 cal yr BP, increasing sediment inputs from the Andes, altering mineral composition, organic matter sources, and soil pH, reflecting rising water levels (Moreira et al., \u003cspan citationid=\"CR56\" class=\"CitationRef\"\u003e2013b\u003c/span\u003e). Geochemical analyses of Preto Lake sediments revealed Solim\u0026otilde;es River hydrological changes during the late Holocene. From 3600\u0026ndash;400 cal yr BP, high river inflow maintained lake stage, contributing smectite-rich sediments and phytoplankton-derived organic matter. Over the last 400 cal yr, reduced river inflow and increased C3-plant runoff marked the lake's semi-isolation, likely caused by sediment accretion forming a natural levee (Moreira et al. \u003cspan citationid=\"CR54\" class=\"CitationRef\"\u003e2020\u003c/span\u003e).\u003c/p\u003e\u003cp\u003eThe \u0026lsquo;Dark Ages Cold Period\u0026rsquo; (DACP) is the most frequently discussed climate anomaly of the first millennium, apparently, it occurred around 1,540 cal BP until 1,175 cal BP. (410 AD and 775 AD). This event has been considered as cold, reported by studies around the Northern Hemisphere (Helama et al. \u003cspan citationid=\"CR34\" class=\"CitationRef\"\u003e2017\u003c/span\u003e). The same authors indicate that DACP might have proceeded because of a combination of factors, particularly volcanic and reduced solar activity.\u003c/p\u003e\u003cp\u003eOver the DACP beginning, the diatoms density records (valves/g) increased like never seen before on Lago Airo and the P:B diatoms ratio resulted on planktonic diatoms dominancy that indicates open water on a lentic system. These results showed that a true lentic system was in formation under this climatic event, that started with a lotic-like system at the beginning (ca. 4,200\u0026ndash;3,600 years BP) of the Late Holocene, being influenced by the Rio Negro floodplain.\u003c/p\u003e\u003cp\u003eAfter 1,000 cal yr BP in an isolate lake from the river influence at Caraj\u0026aacute;s region (Cordeiro et al. \u003cspan citationid=\"CR22\" class=\"CitationRef\"\u003e2008a\u003c/span\u003e) as well as in the floodplain areas of the Amazon River (Moreira et al. 2004), the last 1000 years cal BP were the wettest period on record. This pattern is already identified at low latitudes in the Northern Hemisphere in Roraima Savannas (Beltran et al. \u003cspan citationid=\"CR12\" class=\"CitationRef\"\u003e2023\u003c/span\u003e) that also experienced wetter conditions and established the current lake level and climate after 1000 years.\u003c/p\u003e\u003cp\u003eIn the Amazonas River lowlands, Lake Grande do Curuai and Marac\u0026aacute; Lake showed the higher rate of sedimentation recorded since 2,700 cal yr BP due to the discharge of the Amazon River, caused by changes in ITCZ frequency southwards (Moreira-Turcq et al. \u003cspan citationid=\"CR58\" class=\"CitationRef\"\u003e2014\u003c/span\u003e). According to the planktonic diatoms\u0026rsquo; dominancy interpretation, at DACP Lago Airo water-level started to increase to nowadays\u0026rsquo; levels, which corroborates with the argument of the shift of ITCZ frequency southwards.\u003c/p\u003e\u003cp\u003eIt seems that during the Little Ice Age (ca. 300 years BP) a strong SASM probably reinforced the South Atlantic Subtropical Anticyclone, limiting the southward shift of the ITCZ and bringing drier conditions to northeast Brazil, a mechanism already suggested for the early and middle Holocene (Cruz et al. \u003cspan citationid=\"CR26\" class=\"CitationRef\"\u003e2009\u003c/span\u003e) and for the late Holocene (Novello et al. \u003cspan citationid=\"CR61\" class=\"CitationRef\"\u003e2012\u003c/span\u003e). P:B diatoms ratios on Lago Airo indicated that LIA had less wet conditions than DACP and MCA (See Fig.\u0026nbsp;\u003cspan refid=\"Fig9\" class=\"InternalRef\"\u003e8\u003c/span\u003e). In Fig.\u0026nbsp;\u003cspan refid=\"Fig9\" class=\"InternalRef\"\u003e8\u003c/span\u003e we presented a plot of humid and dry periods from the end of Pleistocene to Holocene on South America latitudinal geographic coordinates 4\u0026ordm;N to 8\u0026ordm;S according to several authors.\u003c/p\u003e\u003cp\u003e\u003c/p\u003e\u003c/div\u003e\u003c/div\u003e"},{"header":"6. Conclusion","content":"\u003cp\u003eThe integration of diatom assemblages, sedimentological data, and geochemical indicators reveals a clear trajectory of environmental change in Lago Airo over the past 13,300 years, shedding light on paleohydrological variability in the Amazonian Rio Negro basin. This provides valuable insights into the climatic variability from the end of the Pleistocene to the Holocene in the Amazon basin. The integration of the data highlights the lake's ecological dynamics.\u003c/p\u003e\u003cp\u003eOver the past 13,300 years, Lago Airo has undergone significant environmental changes driven by hydrological and sedimentary dynamics. During Phase IV (ca. 13,400\u0026ndash;11,800 cal yr BP), the lake exhibited high-energy lotic conditions, dominated by oligotrophic and periphytic species such as \u003cem\u003eEunotia hirudo\u003c/em\u003e and \u003cem\u003eE. bilunaris\u003c/em\u003e, with coarse sediments and low diatom density reflecting strong fluvial influence and low nutrient availability. In Phase III (11,800\u0026ndash;8,800 cal yr BP), the system transitioned toward more stable conditions, marked by increased clay content, slightly higher TOC values, and continued dominance of periphytic diatoms. These changes indicate reduced fluvial influence, moderate detrital input, and a gradual shift toward greater sedimentation stability and slightly higher productivity. Phase II (8,800\u0026ndash;4,100 cal yr BP) saw the emergence of planktonic species like \u003cem\u003eAulacoseira\u003c/em\u003e spp., signaling a transition to more lentic conditions with diminished Rio Negro influence. This phase aligns with mid-Holocene dry conditions, lower lake levels, and increased organic input with terrestrial source. By Phase I, 4,100 cal yr BP to present, the lake had fully transitioned to a lentic environment, characterized by finer sediments, higher diatom density, and dominance of planktonic species. This phase reflects eutrophic conditions, increased productivity, and the wettest climatic period in Amazonia over the past millennia, promoting ecosystem stabilization and organic matter accumulation.\u003c/p\u003e\u003cp\u003eThis hydrological variability indicates a dry early-mid-Holocene climate with extreme events at the Medium Rio negro Basin compared to a wetter and more stable late Holocene, which would be attributed to shifts in the position of the ITCZ and South American Monsoon System.\u003c/p\u003e\u003cp\u003eAround 4,000 years ago, under a wetter climate and more regular rainfall, Lago Airo transitioned to a lentic environment as a result of this new hydroclimatic regime, establishing more eutrophic conditions characterized by finer sediments, higher diatom density, and increased productivity. This contrasts with the Middle Holocene, when the Rio Negro likely transported coarser sediments during extreme climatic events.\u003c/p\u003e\u003cp\u003eOur study showed lower levels of water along with less influence of Rio Negro water\u0026rsquo;s on Lago Airo from 13,400 years to 4,100 years BP (Phases IV, III, and II, which could be inferred by fewer diatom valves/g, increasing percentages of silt, high C/N ratio and low values of δ15N. Whereas from 4,000 years BP to recently (Phase 1, the Rio Negro disconnected from Lago Airo and an increase in water level occurred, characterized by higher valves/g, planktonic taxa dominance, and δ\u003csup\u003e15\u003c/sup\u003eN values increase.\u003c/p\u003e"},{"header":"Declarations","content":"\u003ch2\u003eAuthor Contribution\u003c/h2\u003e\u003cp\u003eLRP, JCCV, DFG, DF, and RCC: wrote the main manuscript textPM-T, BT, LSM, and RCC: conceptualization JDADC, GSM, LRP, JCCV, DFG, DF, AB: Data curation , Formal analysis\u003c/p\u003e\u003ch2\u003eAcknowledgement\u003c/h2\u003e\u003cp\u003eWe are grateful for the support of the Bilateral Scientific Cooperation Project IRD-Fran\u0026ccedil;a/CNPq, Laborat\u0026oacute;rio Mixto Internacional PALEOTRACES and the \"INSU-EC2CO Dynamique du carbone et les changements climatiques dans le Bassin Amazonien. The authors acknowledge research support from the \u0026ldquo;Conselho Nacional de Pesquisa\u0026rdquo; (CNPq) process: 3479873/2013-5, 308222/2015-6 and \u0026ldquo;Funda\u0026ccedil;\u0026atilde;o de Amparo \u0026agrave; Pesquisa do Estado do Rio de Janeiro\u0026rdquo; (FAPERJ) process: E-26/111.625/2011. Also, for the financial aid of the Project \"RED0026/2014 (Fapesb) - Reconstru\u0026ccedil;\u0026atilde;o Paleoambiental da Ba\u0026iacute;a de Camamu: Implica\u0026ccedil;\u0026otilde;es para a Gest\u0026atilde;o Ecossistema\" and for the support of the EcoPaleo lab (Biology Institute, UFBA, BR). We are thankful for the assistance of Dr. Abdelfettah Sifeddine (IRD, France), and Funda\u0026ccedil;\u0026atilde;o Oswaldo Cruz - Fiocruz (Salvador/BA, BR). This study was financed in part by the Coordena\u0026ccedil;\u0026atilde;o de Aperfei\u0026ccedil;oamento de Pessoal de N\u0026iacute;vel Superior - Brasil (CAPES) - Finance Code 001. We also acknowledge the Conselho Nacional de Desenvolvimento Cient\u0026iacute;fico e Tecnol\u0026oacute;gico (CNPq, Brazil) for a Research Productivity Fellowship (PQ)\u003c/p\u003e"},{"header":"References","content":"\u003col\u003e\n\u003cli\u003eAbsy, ML, Cleff, A, Fournier, M, Martin, L, Servant, M, Sifeddine, A, Ferreira DA Silva,M, Soubies, F, Suguio, K, Turcq, B, Van Der Hammen, TH, (1991) Mise en \u0026eacute;vidence dequatre phases d\u0026apos;ouverture de la for\u0026ecirc;t dense dans le sud-est de l\u0026apos;Amazonie au coursdes 60000 derni\u0026egrave;res ann\u0026eacute;es. P Premi\u0026egrave;re comparaison avec d\u0026apos;autres r\u0026eacute;gions tropicales.C.R. Acad. Sci. 312, 673\u0026ndash;678.\u003c/li\u003e\n\u003cli\u003eAlizadeh K, Matthias I, Rodr\u0026iacute;guez-Zorro PA, et al (2017) Forest-savanna boundary shift on the plateau of Serra Sul dos Caraj\u0026aacute;s (southeastern Amazonia) since the mid-Holocene; driving forces and limiting factors. 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Quat Res 1\u0026ndash;15\u003c/li\u003e\n\u003cli\u003eRound FE, Crawford RM, Mann DG (1990) The Diatoms: biology and morphology of the genera. Cambridge University Press, New York. pp 747\u003c/li\u003e\n\u003cli\u003eSanger JE, Gorham E (1972) Stratigraphy of fossil pigments as guide to the postglacial history of Kirchner marsh, Minesota. Limnology and Oceanography 17: 840-854\u003c/li\u003e\n\u003cli\u003eSifeddine, A.; Martin, L.; Turcq, B.; Volkmer-Ribeiro, C.; Soubi\u0026egrave;s, F.; Cordeiro, R. C.; Suguiu, K. (2001). Variations of the Amazonian rainforest environment: a sedimentological record covering 30,000 years. Palaeogeography, Palaeoclimatology, Palaeoecology 168: 221-235.\u003c/li\u003e\n\u003cli\u003eSilva Dias PL, Turcq B, Silva Dias MAF, Braconnot P, Jorgetti T (2009) Mid-Holocene Climate of Tropical South America: A Model-Data Approach. In: Vimeux F., Sylvestre F., Khodri M. (eds) Past Climate Variability in South America and Surrounding Regions. Developments in Paleoenvironmental Research, vol 14. Springer, Dordrecht.\u003c/li\u003e\n\u003cli\u003eSilva M, Cohen M, Rossetti D, Pessenda L (2018). Did Sea-Level Changes Affect the Brazilian Amazon Forest during the Holocene? Radiocarbon 60: 91-112\u003c/li\u003e\n\u003cli\u003eSim\u0026otilde;es Filho, F.F.L., 2000. Sedimenta\u0026ccedil;\u0026atilde;o lacustre e implica\u0026ccedil;\u0026otilde;es paleoambientais na regi\u0026atilde;o de contato floresta-savana de Roraima durante o Holoceno. (Ph.D. thesis) Universidade Federal Fluminense, Niter\u0026oacute;i, Brazil.\u003c/li\u003e\n\u003cli\u003eSoubies F (1980) Existence d\u0026apos;une phase s\u0026egrave;che en Amazonic Br\u0026eacute;silien ne dat\u0026eacute;e para la presenze de charbons dans les sols (6000-3000 anos B.P.). Cah. O. R. S.T. O. Pi., S\u0026eacute;r G\u0026eacute;ol: 11:133-146.\u003c/li\u003e\n\u003cli\u003eSpaulding, S.A.; Potapova, M.G.; Bishop, I.W.; Lee, S.S.; Gasperak, T.S.; Jovanoska, E.; Furey, P.C.; Edlund, M.B. 2021. Diatoms.org: supporting taxonomists, connecting communities. \u003cem\u003eDiatom Research\u003c/em\u003e,\u003cem\u003e \u003c/em\u003e36: 4, 291-304, DOI: 10.1080/0269249X.2021.2006790.\u003c/li\u003e\n\u003cli\u003eStuiver M, Reimer PJ, Reimer RW (2018) CALIB 7.1 [WWW program] at http://calib.org, accessed 2018-6-20.\u003c/li\u003e\n\u003cli\u003eWeng C, Bush MB, Athens JS (2002) Two histories of climate change and hydrarch succession in Ecuadorian Amazonia. Review of Palynology and Paleobotany 120: 73- 90\u003c/li\u003e\n\u003cli\u003eWerth D, Avissar R (2002) The local and global effects of Amazon deforestation, J Geophys Res Atmospheres, 107(D20), LBA-55\u003c/li\u003e\n\u003cli\u003eWolin JA, Stone JR (2010) Diatoms as indicators of water level change in freshwater lakes In:Smol JP, Eugene FS (eds) The diatoms: applications for the environmental and earth science. Cambridge University Press, United Kingdom\u003c/li\u003e\n\u003c/ol\u003e"}],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":true,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":false,"highlight":"","institution":"","isAcceptedByJournal":true,"isAuthorSuppliedPdf":false,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":false,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"journal-of-paleolimnology","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":false,"externalIdentity":"jopl","sideBox":"Learn more about [Journal of Paleolimnology](http://link.springer.com/journal/10933)","snPcode":"10933","submissionUrl":"https://submission.nature.com/new-submission/10933/3","title":"Journal of Paleolimnology","twitterHandle":"","acdcEnabled":true,"dfaEnabled":true,"editorialSystem":"em","reportingPortfolio":"Springer Hybrid","inReviewEnabled":true,"inReviewRevisionsEnabled":false},"keywords":"Pleistocene, Holocene, Paleolimnology, Eunotia spp, Aulacoseira spp, geochemistry, Rio Negro","lastPublishedDoi":"10.21203/rs.3.rs-7687264/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-7687264/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003eThe study of paleolimnological and paleoclimatic variations in the Amazon from the last 13,300 cal yr BP is essential for interpreting the climatic and environmental history of this region, as it is one of the largest and most diverse biomes on the planet. Diatom assembly (density and relative abundance), sedimentological, bulk, and isotopic organic geochemistry analyses were obtained from a 146 cm-long core collected in a marginal lake nowadays disconnected from Rio Negro (Lago Airo; 00\u0026ordm;19\u0026rsquo;37.225\u0026rsquo;\u0026rsquo;S, 66\u0026ordm;08\u0026rsquo;33.266\u0026rsquo;\u0026rsquo;W). Seven organic matter samples were dated by using AMS radiocarbon determination. The 73 species found mostly comprised acidophilic species. We discerned four main phases: Phase IV, from ca. 13,400 cal yr BP to ca 11,800 cal yr BP: diatoms density was relatively low (i.e., ranging from 88 to 37.5 x 10\u0026sup3; valves/g) with mainly periphytic taxa. Sediments are comprised of coarser grains (sand\u0026thinsp;\u0026gt;\u0026thinsp;99%). Geochemical data shows a TOC average of 2.2%, the C/N ratio is higher (average\u0026thinsp;=\u0026thinsp;52.1), and chlorophyll derivatives are lower (average\u0026thinsp;=\u0026thinsp;5.6 SPDU). 15N values show a source related to nitrogen atmospheric fixation metabolism, probably due to the extremely oligotrophic nature of the system. Our results suggest that Phase IV represented the Lago Airo as a lotic-like ecosystem with connections with the Rio Negro, characterized by high energy and thereby low diatom sedimentation. Phase III: 11800 to 8800 cal yr BP: The diatom community kept characteristics of low density with the domain of Eunotia spp (E. hirudo, E. bilunaris, and E. floweri). This phase exhibits a significant increase in clay content of around an order of magnitude, rising from 0.256% in phase IV to 2.05% in phase III. Although the absolute values are still low, they still constitute a sandy-textured sediment. TOC values increased compared to the previous phase, reaching 2.39%, with an increase in the C/N ratio denoting the transport of detrital organic matter from the basins. Phase II: 8800 to 4100 cal yr BP: The diatom community keep characteristics of phase III. This phase exhibits a decrease in clay content to 0.299% with a progressive increase in TOC, compared to the previous phase, reaching 3.47%. This increase in C/N ratio indicates the transport of detrital organic matter from the basins. On the onset of phase I, the Rio Negro became disconnected from Lago Airo, which is suggested by the predominance of silt, associated with low hydrodynamics, and an increase in diatom valve density. Phase I: 4100 to actual: This phase shows an increased diatom valves density (between 30.9 x 104 and 14.1 x 106), composed mainly by planktonic taxa. Silt became dominant (average\u0026thinsp;=\u0026thinsp;59.1; clay\u0026thinsp;=\u0026thinsp;10.2) simultaneously with. Higher silt contribution with a pronounced decrease in C/N ratios (average\u0026thinsp;=\u0026thinsp;34.9) is indicative of declining influence of the Rio Negro over Lago Airo and an increase in autochthonous production, represented by the increase in TOC, changing from 3.47 in phase II to 25.0%, and chlorophyll derivatives (average\u0026thinsp;=\u0026thinsp;12.7 SPDU). Our results suggest an increase in the mean water level in Lago Airo (characterized by higher valves/g, planktonic taxa dominance - Aulacoseira spp. - A. distans and A. granulata, mainly, and δ15N values increase), which was probably the result of higher precipitation regimes in the South American Monsoon System.\u003c/p\u003e","manuscriptTitle":"Paleoclimate variations in Western Amazon based on Lago Airo (Brazil) diatoms from the last 13,300 years cal BP","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2025-10-16 03:02:18","doi":"10.21203/rs.3.rs-7687264/v1","editorialEvents":[{"type":"communityComments","content":0},{"type":"decision","content":"Revision requested","date":"2025-10-29T15:38:34+00:00","index":"","fulltext":""},{"type":"editorInvitedReview","content":"","date":"2025-10-29T12:47:31+00:00","index":"hide","fulltext":""},{"type":"editorInvitedReview","content":"","date":"2025-10-27T10:27:59+00:00","index":"hide","fulltext":""},{"type":"editorInvitedReview","content":"","date":"2025-10-09T02:45:46+00:00","index":"hide","fulltext":""},{"type":"reviewerAgreed","content":"335704560740216014311187325372854952870","date":"2025-10-06T13:49:32+00:00","index":"hide","fulltext":""},{"type":"reviewerAgreed","content":"268204082741923248907599172496029890892","date":"2025-10-03T14:59:57+00:00","index":"hide","fulltext":""},{"type":"reviewerAgreed","content":"122209391769004469468041862846116322782","date":"2025-10-03T13:31:57+00:00","index":"hide","fulltext":""},{"type":"reviewerAgreed","content":"7050513015483048220732622248400983602","date":"2025-10-02T02:09:19+00:00","index":"hide","fulltext":""},{"type":"reviewersInvited","content":"","date":"2025-10-01T18:23:24+00:00","index":"","fulltext":""},{"type":"editorAssigned","content":"","date":"2025-09-24T16:50:09+00:00","index":"","fulltext":""},{"type":"checksComplete","content":"","date":"2025-09-24T16:49:28+00:00","index":"","fulltext":""},{"type":"submitted","content":"Journal of Paleolimnology","date":"2025-09-22T17:57:25+00:00","index":"","fulltext":""}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"journal-of-paleolimnology","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":false,"externalIdentity":"jopl","sideBox":"Learn more about [Journal of Paleolimnology](http://link.springer.com/journal/10933)","snPcode":"10933","submissionUrl":"https://submission.nature.com/new-submission/10933/3","title":"Journal of Paleolimnology","twitterHandle":"","acdcEnabled":true,"dfaEnabled":true,"editorialSystem":"em","reportingPortfolio":"Springer Hybrid","inReviewEnabled":true,"inReviewRevisionsEnabled":false}}],"origin":"","ownerIdentity":"5df8f6a8-4487-4ee2-bf4b-2296ee956568","owner":[],"postedDate":"October 16th, 2025","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"under-review","subjectAreas":[],"tags":[],"updatedAt":"2026-05-20T15:24:23+00:00","versionOfRecord":[],"versionCreatedAt":"2025-10-16 03:02:18","video":"","vorDoi":"","vorDoiUrl":"","workflowStages":[]},"version":"v1","identity":"rs-7687264","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-7687264","identity":"rs-7687264","version":["v1"]},"buildId":"8U1c8b4HqxoKbykW_rLl7","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}