Identify a novel cuproptosis-related prognostic signature and build its ceRNA net and candidate  medicine active ingredients in Pancreatic cancer

Pancreatic cancer (PAAD) is a common malignancy that is difficult to diagnose and treat.Regulation of cell death plays an important role in tumorigenesis and is an important target of almost all therapeutic strategies. Various current studies have proved that various types of cell death patterns are closely related to the elimination of cancer.And here's the latest research is ”cuprotosis”.Cuproptosis is a novel form of programmed cell death, and its mechanism in tumours remains unclear.In the current study, we found that LIPT1、LIAS、DLD、DLAT、PDHA1 and PDHB in 10 differentially expressed CRGs were central genes.GO and KEGG enrichment results showed that these 10 CRGs were mainly enriched in Protein digestion and absorption、Pancreatic secretion、Human papillomavirus infection and Focal adhesion.We constructed a prognostic model based on the DLAT gene and showed good predictive potential.Clinical correlation analysis showed that DLAT was correlated with pathological stage and T stage of PAAD patients.Finally, we constructed a ceRNA network that may play a key role in the progression of PAAD and screened 12 compound active components that may regulate DLAT.This was verified by molecular docking.To sum up, we analyzed the potential value of DLAT gene in PAAD, constructed ceRNA network, and conducted targeted regulation of the active ingredients through comprehensive bioinformatics combined with experimental verification strategies.This work not only provides new insights into the treatment of PAAD, but also provides a basis for the development of new immunotherapeutic drugs targeting cuprotosis.