Efficient targeted mutagenesis by endogenous promoter controlled tRNA-gRNA array of CRISPR/Cas9 in potato and application in Solanum etuberosum

Abstract Here, we developed an endogenous promoter controlled polycistronic tRNA-gRNA (PTG) array of CRISPR/Cas9 in potato for efficient multiplex mutagenesis. The editing efficiency was significantly improved in potato combined with short-term heat treatment. In addition, our use of CRISPR/Cas9 to engineer mutations in wild solanum species demonstrates a high editing efficiency of the developed PTG/Cas9 in Solanum etuberosum, which contributes to develop Solanum etuberosum as a new model for functional genetics research in solanum.